The Alignment Annotations File
Alignment annotations can be imported onto an alignment since version 2.08 of Jalview, via an annotations file. It is a simple ASCII text file consisting of tab delimited records similar to the Sequence Features File, and introduced primarily for use with the Jalview applet.
Alignment annotations files are imported into Jalview in the
following ways:
-annotations <Annotations filename>
Annotations File Format
The File consists of lines containing an instruction followed by
tab delimited fields, and any lines starting with "#" are
ignored. The first non-commented out line of a valid Annotations file must begin with :JALVIEW_ANNOTATION
A row of annotation is added with a line like
GRAPH_TYPE Label Values
You can associate an annotation with a sequence by preceding its
definition with the line:
SEQUENCE_REF seq_name [startIndex]
All Annotations defined after a SEQUENCE_REF command will then be
associated with that sequence, and the first field in the Value
field list will (optionally) be
placed at the startIndex'th column.
Sequence associations
are turned off for subsequent annotation definitions by:
SEQUENCE_REF ALIGNMENT
LINE_GRAPH type annotations can be given a colour,
combined onto the same vertical axis, and have abscissa (lines of
constant value - so they are horizontal) at particular
values using the following lines (respectively):
COLOUR graph name colour
COMBINE graph 1 name graph 2 name
GRAPHLINE graph name value label colour
An example Annotation file is given below:
#Comment lines follow the hash symbol
JALVIEW_ANNOTATION
SEQUENCE_REF FER1_MESCR 5
BAR_GRAPH Bar Graph 1 ||-100,-|-200,-|-300,-|-400,-|200,+|300,+|150,+
LINE_GRAPH Green Values 1.1|2.2|1.3|3.4|0.7|1.4|3.3|2.2|2.1|-1.1|3.2
LINE_GRAPH Red Values 2.1|3.2|1.3|-1.4|5.5|1.4|1.3|4.2|-1.1|1.1|3.2
BAR_GRAPH Bar Graph 2 1,.|2,*|3,:|4,.|5,*|4,:|3,.|2|1|1|2|3|4|5|4
NO_GRAPH Icons ||||E,Sheet1|E|E||||H,Sheet 2|H|H|H||||||
NO_GRAPH Purple Letters m|y|p|r|o|t|e|i|n
COLOUR Bar Graph 2 blue
COLOUR Red Values 255,0,0
COLOUR Green Values green
COLOUR Purple Letters 151,52,228
COMBINE Green Values Red Values
GRAPHLINE Red Values 2.6 threshold black