/* * Jalview - A Sequence Alignment Editor and Viewer (Version 2.7) * Copyright (C) 2011 J Procter, AM Waterhouse, J Engelhardt, LM Lui, G Barton, M Clamp, S Searle * * This file is part of Jalview. * * Jalview is free software: you can redistribute it and/or * modify it under the terms of the GNU General Public License * as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. * * Jalview is distributed in the hope that it will be useful, but * WITHOUT ANY WARRANTY; without even the implied warranty * of MERCHANTABILITY or FITNESS FOR A PARTICULAR * PURPOSE. See the GNU General Public License for more details. * * You should have received a copy of the GNU General Public License along with Jalview. If not, see . */ /* Author: Lauren Michelle Lui * Methods are based on RALEE methods http://personalpages.manchester.ac.uk/staff/sam.griffiths-jones/software/ralee/ * */ package jalview.analysis; import java.util.ArrayList; import java.util.Hashtable; import java.util.Vector; import jalview.datamodel.SequenceFeature; public class Rna { static Hashtable pairHash = new Hashtable(); /** * Based off of RALEE code ralee-get-base-pairs. Keeps track of open bracket * positions in "stack" vector. When a close bracket is reached, pair this * with the last element in the "stack" vector and store in "pairs" vector. * Remove last element in the "stack" vector. Continue in this manner until * the whole string is processed. * * @param line * Secondary structure line of an RNA Stockholm file * @return Array of SequenceFeature; type = RNA helix, begin is open base * pair, end is close base pair */ public static SequenceFeature[] GetBasePairs(String line) { Vector stack = new Vector(); Vector pairs = new Vector(); int i = 0; while (i < line.length()) { char base = line.charAt(i); if ((base == '<') || (base == '(') || (base == '{') || (base == '[')) { stack.addElement(i); } else if ((base == '>') || (base == ')') || (base == '}') || (base == ']')) { Object temp = stack.lastElement(); stack.remove(stack.size() - 1); pairs.addElement(temp); pairs.addElement(i); } i++; } int numpairs = pairs.size() / 2; SequenceFeature[] outPairs = new SequenceFeature[numpairs]; // Convert pairs to array for (int p = 0; p < pairs.size(); p += 2) { int begin = Integer.parseInt(pairs.elementAt(p).toString()); int end = Integer.parseInt(pairs.elementAt(p + 1).toString()); outPairs[p / 2] = new SequenceFeature("RNA helix", "", "", begin, end, ""); //pairHash.put(begin, end); } return outPairs; } /** * Function to get the end position corresponding to a given start position * @param indice - start position of a base pair * @return - end position of a base pair */ /*makes no sense at the moment :( public int findEnd(int indice){ //TODO: Probably extend this to find the start to a given end? //could be done by putting everything twice to the hash ArrayList pair = new ArrayList(); return pairHash.get(indice); }*/ /** * Figures out which helix each position belongs to and stores the helix * number in the 'featureGroup' member of a SequenceFeature Based off of RALEE * code ralee-helix-map. * * @param pairs * Array of SequenceFeature (output from Rna.GetBasePairs) */ public static void HelixMap(SequenceFeature[] pairs) { int helix = 0; // Number of helices/current helix int lastopen = 0; // Position of last open bracket reviewed int lastclose = 9999999; // Position of last close bracket reviewed int i = pairs.length; // Number of pairs int open; // Position of an open bracket under review int close; // Position of a close bracket under review int j; // Counter Hashtable helices = new Hashtable(); // Keep track of helix number for each // position // Go through each base pair and assign positions a helix for (i = 0; i < pairs.length; i++) { open = pairs[i].getBegin(); close = pairs[i].getEnd(); // System.out.println("open " + open + " close " + close); // System.out.println("lastclose " + lastclose + " lastopen " + lastopen); // we're moving from right to left based on closing pair /* * catch things like <<..>>..<<..>> | */ if (open > lastclose) { helix++; } /* * catch things like <<..<<..>>..<<..>>>> | */ j = pairs.length - 1; while (j >= 0) { int popen = pairs[j].getBegin(); // System.out.println("j " + j + " popen " + popen + " lastopen " // +lastopen + " open " + open); if ((popen < lastopen) && (popen > open)) { if (helices.containsValue(popen) && (((Integer) helices.get(popen)) == helix)) { continue; } else { helix++; break; } } j -= 1; } // Put positions and helix information into the hashtable helices.put(open, helix); helices.put(close, helix); // Record helix as featuregroup pairs[i].setFeatureGroup(Integer.toString(helix)); lastopen = open; lastclose = close; } } }