/* * Jalview - A Sequence Alignment Editor and Viewer (Version 2.7) * Copyright (C) 2011 J Procter, AM Waterhouse, G Barton, M Clamp, S Searle * * This file is part of Jalview. * * Jalview is free software: you can redistribute it and/or * modify it under the terms of the GNU General Public License * as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. * * Jalview is distributed in the hope that it will be useful, but * WITHOUT ANY WARRANTY; without even the implied warranty * of MERCHANTABILITY or FITNESS FOR A PARTICULAR * PURPOSE. See the GNU General Public License for more details. * * You should have received a copy of the GNU General Public License along with Jalview. If not, see . */ package jalview.appletgui; import java.util.*; import java.awt.*; import java.awt.event.*; import jalview.analysis.*; import jalview.datamodel.*; public class PCAPanel extends EmbmenuFrame implements Runnable, ActionListener, ItemListener { PCA pca; int top; RotatableCanvas rc; AlignViewport av; SequenceI[] seqs; AlignmentView seqstrings; public PCAPanel(AlignViewport av) { try { jbInit(); } catch (Exception e) { e.printStackTrace(); } for (int i = 1; i < 8; i++) { xCombobox.addItem("dim " + i); yCombobox.addItem("dim " + i); zCombobox.addItem("dim " + i); } this.av = av; seqstrings = av.getAlignmentView(av.getSelectionGroup() != null); if (av.getSelectionGroup() == null) { seqs = av.alignment.getSequencesArray(); } else { seqs = av.getSelectionGroup().getSequencesInOrder(av.alignment); } SeqCigar sq[] = seqstrings.getSequences(); int length = sq[0].getWidth(); for (int i = 0; i < seqs.length; i++) { if (sq[i].getWidth() != length) { System.out .println("Sequences must be equal length for PCA analysis"); return; } } rc = new RotatableCanvas(av); embedMenuIfNeeded(rc); add(rc, BorderLayout.CENTER); jalview.bin.JalviewLite.addFrame(this, "Principal component analysis", 400, 400); Thread worker = new Thread(this); worker.start(); } /** * DOCUMENT ME! */ public void run() { pca = new PCA(seqstrings.getSequenceStrings(' ')); pca.run(); // Now find the component coordinates int ii = 0; while ((ii < seqs.length) && (seqs[ii] != null)) { ii++; } double[][] comps = new double[ii][ii]; for (int i = 0; i < ii; i++) { if (pca.getEigenvalue(i) > 1e-4) { comps[i] = pca.component(i); } } // //////////////// xCombobox.select(0); yCombobox.select(1); zCombobox.select(2); top = pca.getM().rows - 1; Vector points = new Vector(); float[][] scores = pca.getComponents(top - 1, top - 2, top - 3, 100); for (int i = 0; i < pca.getM().rows; i++) { SequencePoint sp = new SequencePoint(seqs[i], scores[i]); points.addElement(sp); } rc.setPoints(points, pca.getM().rows); rc.repaint(); seqs = null; this.repaint(); } void doDimensionChange() { if (top == 0) { return; } int dim1 = top - xCombobox.getSelectedIndex(); int dim2 = top - yCombobox.getSelectedIndex(); int dim3 = top - zCombobox.getSelectedIndex(); float[][] scores = pca.getComponents(dim1, dim2, dim3, 100); for (int i = 0; i < pca.getM().rows; i++) { ((SequencePoint) rc.points.elementAt(i)).coord = scores[i]; } rc.img = null; rc.rotmat.setIdentity(); rc.initAxes(); rc.paint(rc.getGraphics()); } public void actionPerformed(ActionEvent evt) { if (evt.getSource() == inputData) { showOriginalData(); } else { values_actionPerformed(); } } public void itemStateChanged(ItemEvent evt) { if (evt.getSource() == xCombobox) { xCombobox_actionPerformed(); } else if (evt.getSource() == yCombobox) { yCombobox_actionPerformed(); } else if (evt.getSource() == zCombobox) { zCombobox_actionPerformed(); } } protected void xCombobox_actionPerformed() { doDimensionChange(); } protected void yCombobox_actionPerformed() { doDimensionChange(); } protected void zCombobox_actionPerformed() { doDimensionChange(); } public void values_actionPerformed() { CutAndPasteTransfer cap = new CutAndPasteTransfer(false, null); Frame frame = new Frame(); frame.add(cap); jalview.bin.JalviewLite.addFrame(frame, "PCA details", 500, 500); cap.setText(pca.getDetails()); } void showOriginalData() { // decide if av alignment is sufficiently different to original data to // warrant a new window to be created // create new alignmnt window with hidden regions (unhiding hidden regions // yields unaligned seqs) // or create a selection box around columns in alignment view // test Alignment(SeqCigar[]) char gc = '-'; try { // we try to get the associated view's gap character // but this may fail if the view was closed... gc = av.getGapCharacter(); } catch (Exception ex) { } ; Object[] alAndColsel = seqstrings.getAlignmentAndColumnSelection(gc); if (alAndColsel != null && alAndColsel[0] != null) { Alignment al = new Alignment((SequenceI[]) alAndColsel[0]); AlignFrame af = new AlignFrame(al, av.applet, "Original Data for PCA", false); af.viewport.setHiddenColumns((ColumnSelection) alAndColsel[1]); } } public void labels_itemStateChanged(ItemEvent itemEvent) { rc.showLabels(labels.getState()); } Panel jPanel2 = new Panel(); Label jLabel1 = new Label(); Label jLabel2 = new Label(); Label jLabel3 = new Label(); protected Choice xCombobox = new Choice(); protected Choice yCombobox = new Choice(); protected Choice zCombobox = new Choice(); FlowLayout flowLayout1 = new FlowLayout(); BorderLayout borderLayout1 = new BorderLayout(); MenuBar menuBar1 = new MenuBar(); Menu menu1 = new Menu(); Menu menu2 = new Menu(); protected CheckboxMenuItem labels = new CheckboxMenuItem(); MenuItem values = new MenuItem(); MenuItem inputData = new MenuItem(); private void jbInit() throws Exception { this.setLayout(borderLayout1); jPanel2.setLayout(flowLayout1); jLabel1.setFont(new java.awt.Font("Verdana", 0, 12)); jLabel1.setText("x="); jLabel2.setFont(new java.awt.Font("Verdana", 0, 12)); jLabel2.setText("y="); jLabel3.setFont(new java.awt.Font("Verdana", 0, 12)); jLabel3.setText("z="); jPanel2.setBackground(Color.white); zCombobox.setFont(new java.awt.Font("Verdana", 0, 12)); zCombobox.addItemListener(this); yCombobox.setFont(new java.awt.Font("Verdana", 0, 12)); yCombobox.addItemListener(this); xCombobox.setFont(new java.awt.Font("Verdana", 0, 12)); xCombobox.addItemListener(this); this.setMenuBar(menuBar1); menu1.setLabel("File"); menu2.setLabel("View"); labels.setLabel("Labels"); labels.addItemListener(this); values.setLabel("Output Values..."); values.addActionListener(this); inputData.setLabel("Input Data..."); this.add(jPanel2, BorderLayout.SOUTH); jPanel2.add(jLabel1, null); jPanel2.add(xCombobox, null); jPanel2.add(jLabel2, null); jPanel2.add(yCombobox, null); jPanel2.add(jLabel3, null); jPanel2.add(zCombobox, null); menuBar1.add(menu1); menuBar1.add(menu2); menu2.add(labels); menu1.add(values); menu1.add(inputData); inputData.addActionListener(this); } }