*/
package jalview.analysis;
-import static jalview.io.gff.GffConstants.CLINICAL_SIGNIFICANCE;
+import java.util.ArrayList;
+import java.util.Arrays;
+import java.util.Collection;
+import java.util.Collections;
+import java.util.HashMap;
+import java.util.HashSet;
+import java.util.Iterator;
+import java.util.LinkedHashMap;
+import java.util.List;
+import java.util.Map;
+import java.util.Map.Entry;
+import java.util.NoSuchElementException;
+import java.util.Set;
+import java.util.SortedMap;
+import java.util.TreeMap;
+import jalview.bin.Cache;
import jalview.commands.RemoveGapColCommand;
import jalview.datamodel.AlignedCodon;
import jalview.datamodel.AlignedCodonFrame;
import jalview.datamodel.SequenceGroup;
import jalview.datamodel.SequenceI;
import jalview.datamodel.features.SequenceFeatures;
-import jalview.io.gff.Gff3Helper;
import jalview.io.gff.SequenceOntologyI;
import jalview.schemes.ResidueProperties;
import jalview.util.Comparison;
import jalview.util.IntRangeComparator;
import jalview.util.MapList;
import jalview.util.MappingUtils;
-import jalview.util.StringUtils;
-
-import java.io.UnsupportedEncodingException;
-import java.net.URLEncoder;
-import java.util.ArrayList;
-import java.util.Arrays;
-import java.util.Collection;
-import java.util.Collections;
-import java.util.HashMap;
-import java.util.HashSet;
-import java.util.Iterator;
-import java.util.LinkedHashMap;
-import java.util.List;
-import java.util.Map;
-import java.util.Map.Entry;
-import java.util.NoSuchElementException;
-import java.util.Set;
-import java.util.SortedMap;
-import java.util.TreeMap;
/**
* grab bag of useful alignment manipulation operations Expect these to be
.findMappingsForSequence(cdsSeq, mappings);
for (AlignedCodonFrame mapping : dnaMappings)
{
- SequenceI peptide = mapping.findAlignedSequence(cdsSeq, protein);
+ List<SequenceToSequenceMapping> foundMap=new ArrayList<>();
+ SequenceI peptide = mapping.findAlignedSequence(cdsSeq, protein,foundMap);
if (peptide != null)
{
final int peptideLength = peptide.getLength();
- Mapping map = mapping.getMappingBetween(cdsSeq, peptide);
+ Mapping map = foundMap.get(0).getMapping();
if (map != null)
{
MapList mapList = map.getMap();
cdsSeqs.add(cdsSeq);
- if (!dataset.getSequences().contains(cdsSeqDss))
- {
- // check if this sequence is a newly created one
- // so needs adding to the dataset
- dataset.addSequence(cdsSeqDss);
- }
-
/*
- * add a mapping from CDS to the (unchanged) mapped to range
+ * build the mapping from CDS to protein
*/
List<int[]> cdsRange = Collections
.singletonList(new int[]
MapList cdsToProteinMap = new MapList(cdsRange,
mapList.getToRanges(), mapList.getFromRatio(),
mapList.getToRatio());
- AlignedCodonFrame cdsToProteinMapping = new AlignedCodonFrame();
- cdsToProteinMapping.addMap(cdsSeqDss, proteinProduct,
- cdsToProteinMap);
- /*
- * guard against duplicating the mapping if repeating this action
- */
- if (!mappings.contains(cdsToProteinMapping))
+ if (!dataset.getSequences().contains(cdsSeqDss))
{
- mappings.add(cdsToProteinMapping);
+ /*
+ * if this sequence is a newly created one, add it to the dataset
+ * and made a CDS to protein mapping (if sequence already exists,
+ * CDS-to-protein mapping _is_ the transcript-to-protein mapping)
+ */
+ dataset.addSequence(cdsSeqDss);
+ AlignedCodonFrame cdsToProteinMapping = new AlignedCodonFrame();
+ cdsToProteinMapping.addMap(cdsSeqDss, proteinProduct,
+ cdsToProteinMap);
+
+ /*
+ * guard against duplicating the mapping if repeating this action
+ */
+ if (!mappings.contains(cdsToProteinMapping))
+ {
+ mappings.add(cdsToProteinMapping);
+ }
}
propagateDBRefsToCDS(cdsSeqDss, dnaSeq.getDatasetSequence(),
SequenceI newSeq = null;
- final MapList maplist = mapping.getMap();
- if (maplist.isContiguous() && maplist.isFromForwardStrand())
- {
- /*
- * just a subsequence, keep same dataset sequence
- */
- int start = maplist.getFromLowest();
- int end = maplist.getFromHighest();
- newSeq = seq.getSubSequence(start - 1, end);
- newSeq.setName(seqId);
- }
- else
- {
- /*
- * construct by splicing mapped from ranges
- */
- char[] seqChars = seq.getSequence();
- List<int[]> fromRanges = maplist.getFromRanges();
- int cdsWidth = MappingUtils.getLength(fromRanges);
- char[] newSeqChars = new char[cdsWidth];
+ /*
+ * construct CDS sequence by splicing mapped from ranges
+ */
+ char[] seqChars = seq.getSequence();
+ List<int[]> fromRanges = mapping.getMap().getFromRanges();
+ int cdsWidth = MappingUtils.getLength(fromRanges);
+ char[] newSeqChars = new char[cdsWidth];
- int newPos = 0;
- for (int[] range : fromRanges)
+ int newPos = 0;
+ for (int[] range : fromRanges)
+ {
+ if (range[0] <= range[1])
{
- if (range[0] <= range[1])
+ // forward strand mapping - just copy the range
+ int length = range[1] - range[0] + 1;
+ System.arraycopy(seqChars, range[0] - 1, newSeqChars, newPos,
+ length);
+ newPos += length;
+ }
+ else
+ {
+ // reverse strand mapping - copy and complement one by one
+ for (int i = range[0]; i >= range[1]; i--)
{
- // forward strand mapping - just copy the range
- int length = range[1] - range[0] + 1;
- System.arraycopy(seqChars, range[0] - 1, newSeqChars, newPos,
- length);
- newPos += length;
- }
- else
- {
- // reverse strand mapping - copy and complement one by one
- for (int i = range[0]; i >= range[1]; i--)
- {
- newSeqChars[newPos++] = Dna.getComplement(seqChars[i - 1]);
- }
+ newSeqChars[newPos++] = Dna.getComplement(seqChars[i - 1]);
}
}
}
else
{
- System.err.println(
- "JAL-2154 regression: warning - found (and ignnored a duplicate CDS sequence):"
- + mtch.toString());
+ Cache.log.error(
+ "JAL-2154 regression: warning - found (and ignored) a duplicate CDS sequence:" + mtch.toString());
}
}
}
}
/**
- * Helper method that adds a peptide variant feature. ID and
- * clinical_significance attributes of the dna variant (if present) are copied
- * to the new feature.
- *
- * @param peptide
- * @param peptidePos
- * @param residue
- * @param var
- * @param codon
- * the variant codon e.g. aCg
- * @param canonical
- * the 'normal' codon e.g. aTg
- * @return true if a feature was added, else false
- */
- static boolean addPeptideVariant(SequenceI peptide, int peptidePos,
- String residue, DnaVariant var, String codon, String canonical)
- {
- /*
- * get peptide translation of codon e.g. GAT -> D
- * note that variants which are not single alleles,
- * e.g. multibase variants or HGMD_MUTATION etc
- * are currently ignored here
- */
- String trans = codon.contains("-") ? null
- : (codon.length() > CODON_LENGTH ? null
- : ResidueProperties.codonTranslate(codon));
- if (trans == null)
- {
- return false;
- }
- String desc = canonical + "/" + codon;
- String featureType = "";
- if (trans.equals(residue))
- {
- featureType = SequenceOntologyI.SYNONYMOUS_VARIANT;
- }
- else if (ResidueProperties.STOP.equals(trans))
- {
- featureType = SequenceOntologyI.STOP_GAINED;
- }
- else
- {
- String residue3Char = StringUtils
- .toSentenceCase(ResidueProperties.aa2Triplet.get(residue));
- String trans3Char = StringUtils
- .toSentenceCase(ResidueProperties.aa2Triplet.get(trans));
- desc = "p." + residue3Char + peptidePos + trans3Char;
- featureType = SequenceOntologyI.NONSYNONYMOUS_VARIANT;
- }
- SequenceFeature sf = new SequenceFeature(featureType, desc, peptidePos,
- peptidePos, var.getSource());
-
- StringBuilder attributes = new StringBuilder(32);
- String id = (String) var.variant.getValue(VARIANT_ID);
- if (id != null)
- {
- if (id.startsWith(SEQUENCE_VARIANT))
- {
- id = id.substring(SEQUENCE_VARIANT.length());
- }
- sf.setValue(VARIANT_ID, id);
- attributes.append(VARIANT_ID).append("=").append(id);
- // TODO handle other species variants JAL-2064
- StringBuilder link = new StringBuilder(32);
- try
- {
- link.append(desc).append(" ").append(id).append(
- "|http://www.ensembl.org/Homo_sapiens/Variation/Summary?v=")
- .append(URLEncoder.encode(id, "UTF-8"));
- sf.addLink(link.toString());
- } catch (UnsupportedEncodingException e)
- {
- // as if
- }
- }
- String clinSig = (String) var.variant.getValue(CLINICAL_SIGNIFICANCE);
- if (clinSig != null)
- {
- sf.setValue(CLINICAL_SIGNIFICANCE, clinSig);
- attributes.append(";").append(CLINICAL_SIGNIFICANCE).append("=")
- .append(clinSig);
- }
- peptide.addSequenceFeature(sf);
- if (attributes.length() > 0)
- {
- sf.setAttributes(attributes.toString());
- }
- return true;
- }
-
- /**
* Makes an alignment with a copy of the given sequences, adding in any
* non-redundant sequences which are mapped to by the cross-referenced
* sequences.
{
List<SequenceI> alignedSequences = alignedDatasets
.get(seq.getDatasetSequence());
+ if (alignedSequences.isEmpty())
+ {
+ /*
+ * defensive check - shouldn't happen! (JAL-3536)
+ */
+ continue;
+ }
SequenceI alignedSeq = alignedSequences.get(0);
/*
git://source.jalview.org / jalview.git / blobdiff