temp push
[jalview.git] / src / jalview / bin / Jalview.java
index c1354c7..0ec9ee0 100755 (executable)
  */
 package jalview.bin;
 
+import jalview.api.AlignCalcWorkerI;
+import jalview.api.AlignFrameI;
+import jalview.api.AlignViewportI;
+import jalview.api.JalviewApp;
+import jalview.api.StructureSelectionManagerProvider;
+import jalview.bin.ApplicationSingletonProvider.ApplicationSingletonI;
+import jalview.datamodel.ColumnSelection;
+import jalview.datamodel.HiddenColumns;
+import jalview.datamodel.PDBEntry;
+import jalview.datamodel.SequenceGroup;
+import jalview.datamodel.SequenceI;
 import jalview.ext.so.SequenceOntology;
 import jalview.gui.AlignFrame;
+import jalview.gui.AlignViewport;
+import jalview.gui.AlignmentPanel;
+import jalview.gui.CalculationChooser;
 import jalview.gui.Desktop;
+import jalview.gui.Preferences;
 import jalview.gui.PromptUserConfig;
+import jalview.gui.StructureViewer;
 import jalview.io.AppletFormatAdapter;
 import jalview.io.BioJsHTMLOutput;
 import jalview.io.DataSourceType;
 import jalview.io.FileFormat;
 import jalview.io.FileFormatException;
 import jalview.io.FileFormatI;
+import jalview.io.FileFormats;
 import jalview.io.FileLoader;
 import jalview.io.HtmlSvgOutput;
 import jalview.io.IdentifyFile;
 import jalview.io.NewickFile;
 import jalview.io.gff.SequenceOntologyFactory;
+import jalview.javascript.JSFunctionExec;
+import jalview.javascript.MouseOverStructureListener;
+import jalview.renderer.seqfeatures.FeatureRenderer;
 import jalview.schemes.ColourSchemeI;
 import jalview.schemes.ColourSchemeProperty;
+import jalview.structure.SelectionSource;
+import jalview.structure.VamsasSource;
 import jalview.util.MessageManager;
 import jalview.util.Platform;
 import jalview.ws.jws2.Jws2Discoverer;
 
+import java.applet.AppletContext;
 import java.io.BufferedReader;
 import java.io.File;
 import java.io.FileOutputStream;
@@ -58,16 +81,17 @@ import java.security.PermissionCollection;
 import java.security.Permissions;
 import java.security.Policy;
 import java.util.HashMap;
+import java.util.Hashtable;
 import java.util.Map;
 import java.util.Vector;
 
 import javax.swing.LookAndFeel;
+import javax.swing.SwingUtilities;
 import javax.swing.UIManager;
 
-import com.threerings.getdown.util.LaunchUtil;
-
 import groovy.lang.Binding;
 import groovy.util.GroovyScriptEngine;
+import netscape.javascript.JSObject;
 
 /**
  * Main class for Jalview Application <br>
@@ -84,751 +108,2069 @@ import groovy.util.GroovyScriptEngine;
  * @author $author$
  * @version $Revision$
  */
-public class Jalview
+public class Jalview implements ApplicationSingletonI, JalviewJSApi
 {
-       /*
-        * singleton instance of this class
-        */
-       private static Jalview instance;
-
-       private Desktop desktop;
-
-       public static AlignFrame currentAlignFrame;
-
-       static {
-               // grab all the rights we can the JVM
-               Policy.setPolicy(new Policy() {
-                       @Override
-                       public PermissionCollection getPermissions(CodeSource codesource) {
-                               Permissions perms = new Permissions();
-                               perms.add(new AllPermission());
-                               return (perms);
-                       }
-
-                       @Override
-                       public void refresh() {
-                       }
-               });
-       }
-
-       /**
-        * keep track of feature fetching tasks.
-        * 
-        * @author JimP
-        * 
-        */
-       class FeatureFetcher {
-               /*
-                * TODO: generalise to track all jalview events to orchestrate batch processing
-                * events.
-                */
-
-               private int queued = 0;
-
-               private int running = 0;
-
-               public FeatureFetcher() {
-
-               }
-
-               public void addFetcher(final AlignFrame af, final Vector<String> dasSources) {
-                       final long id = System.currentTimeMillis();
-                       queued++;
-                       final FeatureFetcher us = this;
-                       new Thread(new Runnable() {
-
-                               @Override
-                               public void run() {
-                                       synchronized (us) {
-                                               queued--;
-                                               running++;
-                                       }
-
-                                       af.setProgressBar(MessageManager.getString("status.das_features_being_retrived"), id);
-                                       af.featureSettings_actionPerformed(null);
-                                       af.setProgressBar(null, id);
-                                       synchronized (us) {
-                                               running--;
-                                       }
-                               }
-                       }).start();
-               }
-
-               public synchronized boolean allFinished() {
-                       return queued == 0 && running == 0;
-               }
-
-       }
-
-       public static Jalview getInstance() {
-               return instance;
-       }
-
-       /**
-        * main class for Jalview application
-        * 
-        * @param args open <em>filename</em>
-        */
-       public static void main(String[] args) {
-               instance = new Jalview();
-               instance.doMain(args);
-       }
-
-       /**
-        * @param args
-        */
-       void doMain(String[] args) {
-               System.setSecurityManager(null);
-               System.out.println("Java version: " + System.getProperty("java.version"));
-               System.out.println(System.getProperty("os.arch") + " " + System.getProperty("os.name") + " "
-                               + System.getProperty("os.version"));
-               // report Jalview version
-               Cache.loadBuildProperties(true);
-
-               ArgsParser aparser = new ArgsParser(args);
-               boolean headless = false;
-
-               if (aparser.contains("help") || aparser.contains("h")) {
-                       showUsage();
-                       System.exit(0);
-               }
-               if (aparser.contains("nodisplay") || aparser.contains("nogui") || aparser.contains("headless")) {
-                       System.setProperty("java.awt.headless", "true");
-                       headless = true;
-               }
-               String usrPropsFile = aparser.getValue("props");
-               Cache.loadProperties(usrPropsFile); // must do this before
-               if (usrPropsFile != null) {
-                       System.out.println("CMD [-props " + usrPropsFile + "] executed successfully!");
-               }
-
-               // anything else!
-
-               final String jabawsUrl = aparser.getValue("jabaws");
-               if (jabawsUrl != null) {
-                       try {
-                               Jws2Discoverer.getDiscoverer().setPreferredUrl(jabawsUrl);
-                               System.out.println("CMD [-jabaws " + jabawsUrl + "] executed successfully!");
-                       } catch (MalformedURLException e) {
-                               System.err.println("Invalid jabaws parameter: " + jabawsUrl + " ignored");
-                       }
-               }
-
-               String defs = aparser.getValue("setprop");
-               while (defs != null) {
-                       int p = defs.indexOf('=');
-                       if (p == -1) {
-                               System.err.println("Ignoring invalid setprop argument : " + defs);
-                       } else {
-                               System.out.println("Executing setprop argument: " + defs);
-                               // DISABLED FOR SECURITY REASONS
-                               // TODO: add a property to allow properties to be overriden by cli args
-                               // Cache.setProperty(defs.substring(0,p), defs.substring(p+1));
-                       }
-                       defs = aparser.getValue("setprop");
-               }
-               if (System.getProperty("java.awt.headless") != null && System.getProperty("java.awt.headless").equals("true")) {
-                       headless = true;
-               }
-               System.setProperty("http.agent", "Jalview Desktop/" + Cache.getDefault("VERSION", "Unknown"));
-               try {
-                       Cache.initLogger();
-               } catch (NoClassDefFoundError error) {
-                       error.printStackTrace();
-                       System.out.println("\nEssential logging libraries not found."
-                                       + "\nUse: java -classpath \"$PATH_TO_LIB$/*:$PATH_TO_CLASSES$\" jalview.bin.Jalview");
-                       System.exit(0);
-               }
-
-               desktop = null;
-
-               try {
-                       UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName());
-               } catch (Exception ex) {
-                       System.err.println("Unexpected Look and Feel Exception");
-                       ex.printStackTrace();
-               }
-               if (Platform.isAMac()) {
-
-                       LookAndFeel lookAndFeel = ch.randelshofer.quaqua.QuaquaManager.getLookAndFeel();
-                       System.setProperty("com.apple.mrj.application.apple.menu.about.name", "Jalview");
-                       System.setProperty("apple.laf.useScreenMenuBar", "true");
-                       if (lookAndFeel != null) {
-                               try {
-                                       UIManager.setLookAndFeel(lookAndFeel);
-                               } catch (Throwable e) {
-                                       System.err.println("Failed to set QuaQua look and feel: " + e.toString());
-                               }
-                       }
-                       if (lookAndFeel == null || !(lookAndFeel.getClass().isAssignableFrom(UIManager.getLookAndFeel().getClass()))
-                                       || !UIManager.getLookAndFeel().getClass().toString().toLowerCase().contains("quaqua")) {
-                               try {
-                                       System.err.println(
-                                                       "Quaqua LaF not available on this plaform. Using VAqua(4).\nSee https://issues.jalview.org/browse/JAL-2976");
-                                       UIManager.setLookAndFeel("org.violetlib.aqua.AquaLookAndFeel");
-                               } catch (Throwable e) {
-                                       System.err.println("Failed to reset look and feel: " + e.toString());
-                               }
-                       }
-               }
-
-               /*
-                * configure 'full' SO model if preferences say to, else use the default (SO
-                * Lite)
-                */
-               if (Cache.getDefault("USE_FULL_SO", false)) {
-                       SequenceOntologyFactory.setInstance(new SequenceOntology());
-               }
-
-               if (!headless) {
-                       desktop = new Desktop();
-                       desktop.setInBatchMode(true); // indicate we are starting up
-
-                       try {
-                               JalviewTaskbar.setTaskbar(this);
-                       } catch (Exception e) {
-                               System.out.println("Cannot set Taskbar");
-                               // e.printStackTrace();
-                       } catch (Throwable t) {
-                               System.out.println("Cannot set Taskbar");
-                               // t.printStackTrace();
-                       }
-
-                       desktop.setVisible(true);
-                       desktop.startServiceDiscovery();
-                       if (!aparser.contains("nousagestats")) {
-                               startUsageStats(desktop);
-                       } else {
-                               System.err.println("CMD [-nousagestats] executed successfully!");
-                       }
-
-                       if (!aparser.contains("noquestionnaire")) {
-                               String url = aparser.getValue("questionnaire");
-                               if (url != null) {
-                                       // Start the desktop questionnaire prompter with the specified
-                                       // questionnaire
-                                       Cache.log.debug("Starting questionnaire url at " + url);
-                                       desktop.checkForQuestionnaire(url);
-                                       System.out.println("CMD questionnaire[-" + url + "] executed successfully!");
-                               } else {
-                                       if (Cache.getProperty("NOQUESTIONNAIRES") == null) {
-                                               // Start the desktop questionnaire prompter with the specified
-                                               // questionnaire
-                                               // String defurl =
-                                               // "http://anaplog.compbio.dundee.ac.uk/cgi-bin/questionnaire.pl";
-                                               // //
-                                               String defurl = "http://www.jalview.org/cgi-bin/questionnaire.pl";
-                                               Cache.log.debug("Starting questionnaire with default url: " + defurl);
-                                               desktop.checkForQuestionnaire(defurl);
-                                       }
-                               }
-                       } else {
-                               System.err.println("CMD [-noquestionnaire] executed successfully!");
-                       }
-
-                       if (!aparser.contains("nonews")) {
-                               desktop.checkForNews();
-                       }
-
-                       BioJsHTMLOutput.updateBioJS();
-               }
-
-               // Move any new getdown-launcher-new.jar into place over old
-               // getdown-launcher.jar
-               String appdirString = System.getProperty("getdownappdir");
-               if (appdirString != null && appdirString.length() > 0) {
-                       final File appdir = new File(appdirString);
-                       new Thread() {
-                               @Override
-                               public void run() {
-                                       LaunchUtil.upgradeGetdown(new File(appdir, "getdown-launcher-old.jar"),
-                                                       new File(appdir, "getdown-launcher.jar"), new File(appdir, "getdown-launcher-new.jar"));
-                               }
-                       }.start();
-               }
-
-               String file = null, data = null;
-               FileFormatI format = null;
-               DataSourceType protocol = null;
-               FileLoader fileLoader = new FileLoader(!headless);
-
-               String groovyscript = null; // script to execute after all loading is
-               // completed one way or another
-               // extract groovy argument and execute if necessary
-               groovyscript = aparser.getValue("groovy", true);
-               file = aparser.getValue("open", true);
-
-               if (file == null && desktop == null) {
-                       System.out.println("No files to open!");
-                       System.exit(1);
-               }
-               String vamsasImport = aparser.getValue("vdoc");
-               String vamsasSession = aparser.getValue("vsess");
-               if (vamsasImport != null || vamsasSession != null) {
-                       if (desktop == null || headless) {
-                               System.out.println("Headless vamsas sessions not yet supported. Sorry.");
-                               System.exit(1);
-                       }
-                       // if we have a file, start a new session and import it.
-                       boolean inSession = false;
-                       if (vamsasImport != null) {
-                               try {
-                                       DataSourceType viprotocol = AppletFormatAdapter.checkProtocol(vamsasImport);
-                                       if (viprotocol == DataSourceType.FILE) {
-                                               inSession = desktop.vamsasImport(new File(vamsasImport));
-                                       } else if (viprotocol == DataSourceType.URL) {
-                                               inSession = desktop.vamsasImport(new URL(vamsasImport));
-                                       }
-
-                               } catch (Exception e) {
-                                       System.err.println("Exeption when importing " + vamsasImport + " as a vamsas document.");
-                                       e.printStackTrace();
-                               }
-                               if (!inSession) {
-                                       System.err.println("Failed to import " + vamsasImport + " as a vamsas document.");
-                               } else {
-                                       System.out.println("Imported Successfully into new session "
-                                                       + desktop.getVamsasApplication().getCurrentSession());
-                               }
-                       }
-                       if (vamsasSession != null) {
-                               if (vamsasImport != null) {
-                                       // close the newly imported session and import the Jalview specific
-                                       // remnants into the new session later on.
-                                       desktop.vamsasStop_actionPerformed(null);
-                               }
-                               // now join the new session
-                               try {
-                                       if (desktop.joinVamsasSession(vamsasSession)) {
-                                               System.out.println("Successfully joined vamsas session " + vamsasSession);
-                                       } else {
-                                               System.err.println("WARNING: Failed to join vamsas session " + vamsasSession);
-                                       }
-                               } catch (Exception e) {
-                                       System.err.println("ERROR: Failed to join vamsas session " + vamsasSession);
-                                       e.printStackTrace();
-                               }
-                               if (vamsasImport != null) {
-                                       // the Jalview specific remnants can now be imported into the new
-                                       // session at the user's leisure.
-                                       Cache.log.info("Skipping Push for import of data into existing vamsas session."); // TODO:
-                                       // enable
-                                       // this
-                                       // when
-                                       // debugged
-                                       // desktop.getVamsasApplication().push_update();
-                               }
-                       }
-               }
-               long progress = -1;
-               // Finally, deal with the remaining input data.
-               if (file != null) {
-                       if (!headless) {
-                               desktop.setProgressBar(MessageManager.getString("status.processing_commandline_args"),
-                                               progress = System.currentTimeMillis());
-                       }
-                       System.out.println("CMD [-open " + file + "] executed successfully!");
-
-                       if (!file.startsWith("http://")) {
-                               if (!(new File(file)).exists()) {
-                                       System.out.println("Can't find " + file);
-                                       if (headless) {
-                                               System.exit(1);
-                                       }
-                               }
-                       }
-
-                       protocol = AppletFormatAdapter.checkProtocol(file);
-
-                       try {
-                               format = new IdentifyFile().identify(file, protocol);
-                       } catch (FileFormatException e1) {
-                               // TODO ?
-                       }
-
-                       AlignFrame af = fileLoader.LoadFileWaitTillLoaded(file, protocol, format);
-                       if (af == null) {
-                               System.out.println("error");
-                       } else {
-                               setCurrentAlignFrame(af);
-                               data = aparser.getValue("colour", true);
-                               if (data != null) {
-                                       data.replaceAll("%20", " ");
-
-                                       ColourSchemeI cs = ColourSchemeProperty.getColourScheme(af.getViewport(),
-                                                       af.getViewport().getAlignment(), data);
-
-                                       if (cs != null) {
-                                               System.out.println("CMD [-color " + data + "] executed successfully!");
-                                       }
-                                       af.changeColour(cs);
-                               }
-
-                               // Must maintain ability to use the groups flag
-                               data = aparser.getValue("groups", true);
-                               if (data != null) {
-                                       af.parseFeaturesFile(data, AppletFormatAdapter.checkProtocol(data));
-                                       // System.out.println("Added " + data);
-                                       System.out.println("CMD groups[-" + data + "]  executed successfully!");
-                               }
-                               data = aparser.getValue("features", true);
-                               if (data != null) {
-                                       af.parseFeaturesFile(data, AppletFormatAdapter.checkProtocol(data));
-                                       // System.out.println("Added " + data);
-                                       System.out.println("CMD [-features " + data + "]  executed successfully!");
-                               }
-
-                               data = aparser.getValue("annotations", true);
-                               if (data != null) {
-                                       af.loadJalviewDataFile(data, null, null, null);
-                                       // System.out.println("Added " + data);
-                                       System.out.println("CMD [-annotations " + data + "] executed successfully!");
-                               }
-                               // set or clear the sortbytree flag.
-                               if (aparser.contains("sortbytree")) {
-                                       af.getViewport().setSortByTree(true);
-                                       if (af.getViewport().getSortByTree()) {
-                                               System.out.println("CMD [-sortbytree] executed successfully!");
-                                       }
-                               }
-                               if (aparser.contains("no-annotation")) {
-                                       af.getViewport().setShowAnnotation(false);
-                                       if (!af.getViewport().isShowAnnotation()) {
-                                               System.out.println("CMD no-annotation executed successfully!");
-                                       }
-                               }
-                               if (aparser.contains("nosortbytree")) {
-                                       af.getViewport().setSortByTree(false);
-                                       if (!af.getViewport().getSortByTree()) {
-                                               System.out.println("CMD [-nosortbytree] executed successfully!");
-                                       }
-                               }
-                               data = aparser.getValue("tree", true);
-                               if (data != null) {
-                                       try {
-                                               System.out.println("CMD [-tree " + data + "] executed successfully!");
-                                               NewickFile nf = new NewickFile(data, AppletFormatAdapter.checkProtocol(data));
-                                               af.getViewport().setCurrentTree(af.showNewickTree(nf, data).getTree());
-                                       } catch (IOException ex) {
-                                               System.err.println("Couldn't add tree " + data);
-                                               ex.printStackTrace(System.err);
-                                       }
-                               }
-                               // TODO - load PDB structure(s) to alignment JAL-629
-                               // (associate with identical sequence in alignment, or a specified
-                               // sequence)
-                               if (groovyscript != null) {
-                                       // Execute the groovy script after we've done all the rendering stuff
-                                       // and before any images or figures are generated.
-                                       System.out.println("Executing script " + groovyscript);
-                                       executeGroovyScript(groovyscript, af);
-                                       System.out.println("CMD groovy[" + groovyscript + "] executed successfully!");
-                                       groovyscript = null;
-                               }
-                               String imageName = "unnamed.png";
-                               while (aparser.getSize() > 1) {
-                                       String outputFormat = aparser.nextValue();
-                                       file = aparser.nextValue();
-
-                                       if (outputFormat.equalsIgnoreCase("png")) {
-                                               af.createPNG(new File(file));
-                                               imageName = (new File(file)).getName();
-                                               System.out.println("Creating PNG image: " + file);
-                                               continue;
-                                       } else if (outputFormat.equalsIgnoreCase("svg")) {
-                                               File imageFile = new File(file);
-                                               imageName = imageFile.getName();
-                                               af.createSVG(imageFile);
-                                               System.out.println("Creating SVG image: " + file);
-                                               continue;
-                                       } else if (outputFormat.equalsIgnoreCase("html")) {
-                                               File imageFile = new File(file);
-                                               imageName = imageFile.getName();
-                                               HtmlSvgOutput htmlSVG = new HtmlSvgOutput(af.alignPanel);
-                                               htmlSVG.exportHTML(file);
-
-                                               System.out.println("Creating HTML image: " + file);
-                                               continue;
-                                       } else if (outputFormat.equalsIgnoreCase("biojsmsa")) {
-                                               if (file == null) {
-                                                       System.err.println("The output html file must not be null");
-                                                       return;
-                                               }
-                                               try {
-                                                       BioJsHTMLOutput.refreshVersionInfo(BioJsHTMLOutput.BJS_TEMPLATES_LOCAL_DIRECTORY);
-                                               } catch (URISyntaxException e) {
-                                                       e.printStackTrace();
-                                               }
-                                               BioJsHTMLOutput bjs = new BioJsHTMLOutput(af.alignPanel);
-                                               bjs.exportHTML(file);
-                                               System.out.println("Creating BioJS MSA Viwer HTML file: " + file);
-                                               continue;
-                                       } else if (outputFormat.equalsIgnoreCase("imgMap")) {
-                                               af.createImageMap(new File(file), imageName);
-                                               System.out.println("Creating image map: " + file);
-                                               continue;
-                                       } else if (outputFormat.equalsIgnoreCase("eps")) {
-                                               File outputFile = new File(file);
-                                               System.out.println("Creating EPS file: " + outputFile.getAbsolutePath());
-                                               af.createEPS(outputFile);
-                                               continue;
-                                       }
-
-                                       if (af.saveAlignment(file, format)) {
-                                               System.out.println("Written alignment in " + format + " format to " + file);
-                                       } else {
-                                               System.out.println("Error writing file " + file + " in " + format + " format!!");
-                                       }
-
-                               }
-
-                               while (aparser.getSize() > 0) {
-                                       System.out.println("Unknown arg: " + aparser.nextValue());
-                               }
-                       }
-               }
-               AlignFrame startUpAlframe = null;
-               // We'll only open the default file if the desktop is visible.
-               // And the user
-               // ////////////////////
-
-               if (!headless && file == null && vamsasImport == null
-                               && jalview.bin.Cache.getDefault("SHOW_STARTUP_FILE", true)) {
-                       file = jalview.bin.Cache.getDefault("STARTUP_FILE",
-                                       jalview.bin.Cache.getDefault("www.jalview.org", "http://www.jalview.org")
-                                                       + "/examples/exampleFile_2_7.jar");
-                       if (file.equals("http://www.jalview.org/examples/exampleFile_2_3.jar")) {
-                               // hardwire upgrade of the startup file
-                               file.replace("_2_3.jar", "_2_7.jar");
-                               // and remove the stale setting
-                               jalview.bin.Cache.removeProperty("STARTUP_FILE");
-                       }
-
-                       protocol = DataSourceType.FILE;
-
-                       if (file.indexOf("http:") > -1) {
-                               protocol = DataSourceType.URL;
-                       }
-
-                       if (file.endsWith(".jar")) {
-                               format = FileFormat.Jalview;
-                       } else {
-                               try {
-                                       format = new IdentifyFile().identify(file, protocol);
-                               } catch (FileFormatException e) {
-                                       // TODO what?
-                               }
-                       }
-
-                       startUpAlframe = fileLoader.LoadFileWaitTillLoaded(file, protocol, format);
-                       // extract groovy arguments before anything else.
-               }
-
-               // Once all other stuff is done, execute any groovy scripts (in order)
-               if (groovyscript != null) {
-                       if (Cache.groovyJarsPresent()) {
-                               System.out.println("Executing script " + groovyscript);
-                               executeGroovyScript(groovyscript, startUpAlframe);
-                       } else {
-                               System.err.println("Sorry. Groovy Support is not available, so ignoring the provided groovy script "
-                                               + groovyscript);
-                       }
-               }
-               // and finally, turn off batch mode indicator - if the desktop still exists
-               if (desktop != null) {
-                       if (progress != -1) {
-                               desktop.setProgressBar(null, progress);
-                       }
-                       desktop.setInBatchMode(false);
-               }
-       }
-
-       private static void showUsage() {
-               System.out.println("Usage: jalview -open [FILE] [OUTPUT_FORMAT] [OUTPUT_FILE]\n\n"
-                               + "-nodisplay\tRun Jalview without User Interface.\n"
-                               + "-props FILE\tUse the given Jalview properties file instead of users default.\n"
-                               + "-colour COLOURSCHEME\tThe colourscheme to be applied to the alignment\n"
-                               + "-annotations FILE\tAdd precalculated annotations to the alignment.\n"
-                               + "-tree FILE\tLoad the given newick format tree file onto the alignment\n"
-                               + "-features FILE\tUse the given file to mark features on the alignment.\n"
-                               + "-fasta FILE\tCreate alignment file FILE in Fasta format.\n"
-                               + "-clustal FILE\tCreate alignment file FILE in Clustal format.\n"
-                               + "-pfam FILE\tCreate alignment file FILE in PFAM format.\n"
-                               + "-msf FILE\tCreate alignment file FILE in MSF format.\n"
-                               + "-pileup FILE\tCreate alignment file FILE in Pileup format\n"
-                               + "-pir FILE\tCreate alignment file FILE in PIR format.\n"
-                               + "-blc FILE\tCreate alignment file FILE in BLC format.\n"
-                               + "-json FILE\tCreate alignment file FILE in JSON format.\n"
-                               + "-jalview FILE\tCreate alignment file FILE in Jalview format.\n"
-                               + "-png FILE\tCreate PNG image FILE from alignment.\n"
-                               + "-svg FILE\tCreate SVG image FILE from alignment.\n"
-                               + "-html FILE\tCreate HTML file from alignment.\n"
-                               + "-biojsMSA FILE\tCreate BioJS MSA Viewer HTML file from alignment.\n"
-                               + "-imgMap FILE\tCreate HTML file FILE with image map of PNG image.\n"
-                               + "-eps FILE\tCreate EPS file FILE from alignment.\n"
-                               + "-questionnaire URL\tQueries the given URL for information about any Jalview user questionnaires.\n"
-                               + "-noquestionnaire\tTurn off questionnaire check.\n" + "-nonews\tTurn off check for Jalview news.\n"
-                               + "-nousagestats\tTurn off google analytics tracking for this session.\n"
-                               + "-sortbytree OR -nosortbytree\tEnable or disable sorting of the given alignment by the given tree\n"
-                               // +
-                               // "-setprop PROPERTY=VALUE\tSet the given Jalview property,
-                               // after all other properties files have been read\n\t
-                               // (quote the 'PROPERTY=VALUE' pair to ensure spaces are
-                               // passed in correctly)"
-                               + "-jabaws URL\tSpecify URL for Jabaws services (e.g. for a local installation).\n"
-                               + "-fetchfrom nickname\tQuery nickname for features for the alignments and display them.\n"
-                               // +
-                               // "-vdoc vamsas-document\tImport vamsas document into new
-                               // session or join existing session with same URN\n"
-                               // + "-vses vamsas-session\tJoin session with given URN\n"
-                               + "-groovy FILE\tExecute groovy script in FILE, after all other arguments have been processed (if FILE is the text 'STDIN' then the file will be read from STDIN)\n"
-                               + "\n~Read documentation in Application or visit http://www.jalview.org for description of Features and Annotations file~\n\n");
-       }
-
-       private static void startUsageStats(final Desktop desktop) {
-               /**
-                * start a User Config prompt asking if we can log usage statistics.
-                */
-               PromptUserConfig prompter = new PromptUserConfig(Desktop.desktop, "USAGESTATS", "Jalview Usage Statistics",
-                               "Do you want to help make Jalview better by enabling "
-                                               + "the collection of usage statistics with Google Analytics ?"
-                                               + "\n\n(you can enable or disable usage tracking in the preferences)",
-                               new Runnable() {
-                                       @Override
-                                       public void run() {
-                                               Cache.log.debug("Initialising googletracker for usage stats.");
-                                               Cache.initGoogleTracker();
-                                               Cache.log.debug("Tracking enabled.");
-                                       }
-                               }, new Runnable() {
-                                       @Override
-                                       public void run() {
-                                               Cache.log.debug("Not enabling Google Tracking.");
-                                       }
-                               }, null, true);
-               desktop.addDialogThread(prompter);
-       }
-
-       /**
-        * Locate the given string as a file and pass it to the groovy interpreter.
-        * 
-        * @param groovyscript   the script to execute
-        * @param jalviewContext the Jalview Desktop object passed in to the groovy
-        *                       binding as the 'Jalview' object.
-        */
-       private void executeGroovyScript(String groovyscript, AlignFrame af) {
-               /**
-                * for scripts contained in files
-                */
-               File tfile = null;
-               /**
-                * script's URI
-                */
-               URL sfile = null;
-               if (groovyscript.trim().equals("STDIN")) {
-                       // read from stdin into a tempfile and execute it
-                       try {
-                               tfile = File.createTempFile("jalview", "groovy");
-                               PrintWriter outfile = new PrintWriter(new OutputStreamWriter(new FileOutputStream(tfile)));
-                               BufferedReader br = new BufferedReader(new InputStreamReader(System.in));
-                               String line = null;
-                               while ((line = br.readLine()) != null) {
-                                       outfile.write(line + "\n");
-                               }
-                               br.close();
-                               outfile.flush();
-                               outfile.close();
-
-                       } catch (Exception ex) {
-                               System.err.println("Failed to read from STDIN into tempfile "
-                                               + ((tfile == null) ? "(tempfile wasn't created)" : tfile.toString()));
-                               ex.printStackTrace();
-                               return;
-                       }
-                       try {
-                               sfile = tfile.toURI().toURL();
-                       } catch (Exception x) {
-                               System.err.println(
-                                               "Unexpected Malformed URL Exception for temporary file created from STDIN: " + tfile.toURI());
-                               x.printStackTrace();
-                               return;
-                       }
-               } else {
-                       try {
-                               sfile = new URI(groovyscript).toURL();
-                       } catch (Exception x) {
-                               tfile = new File(groovyscript);
-                               if (!tfile.exists()) {
-                                       System.err.println("File '" + groovyscript + "' does not exist.");
-                                       return;
-                               }
-                               if (!tfile.canRead()) {
-                                       System.err.println("File '" + groovyscript + "' cannot be read.");
-                                       return;
-                               }
-                               if (tfile.length() < 1) {
-                                       System.err.println("File '" + groovyscript + "' is empty.");
-                                       return;
-                               }
-                               try {
-                                       sfile = tfile.getAbsoluteFile().toURI().toURL();
-                               } catch (Exception ex) {
-                                       System.err.println("Failed to create a file URL for " + tfile.getAbsoluteFile());
-                                       return;
-                               }
-                       }
-               }
-               try {
-                       Map<String, java.lang.Object> vbinding = new HashMap<>();
-                       vbinding.put("Jalview", this);
-                       if (af != null) {
-                               vbinding.put("currentAlFrame", af);
-                       }
-                       Binding gbinding = new Binding(vbinding);
-                       GroovyScriptEngine gse = new GroovyScriptEngine(new URL[] { sfile });
-                       gse.run(sfile.toString(), gbinding);
-                       if ("STDIN".equals(groovyscript)) {
-                               // delete temp file that we made -
-                               // only if it was successfully executed
-                               tfile.delete();
-                       }
-               } catch (Exception e) {
-                       System.err.println("Exception Whilst trying to execute file " + sfile + " as a groovy script.");
-                       e.printStackTrace(System.err);
-
-               }
-       }
-
-       public static boolean isHeadlessMode() {
-               String isheadless = System.getProperty("java.awt.headless");
-               if (isheadless != null && isheadless.equalsIgnoreCase("true")) {
-                       return true;
-               }
-               return false;
-       }
-
-       public AlignFrame[] getAlignFrames() {
-               return desktop == null ? new AlignFrame[] { getCurrentAlignFrame() } : Desktop.getAlignFrames();
-
-       }
-
-       /**
-        * Quit method delegates to Desktop.quit - unless running in headless mode when
-        * it just ends the JVM
-        */
-       public void quit() {
-               if (desktop != null) {
-                       desktop.quit();
-               } else {
-                       System.exit(0);
-               }
-       }
-
-       public static AlignFrame getCurrentAlignFrame() {
-               return Jalview.currentAlignFrame;
-       }
-
-       public static void setCurrentAlignFrame(AlignFrame currentAlignFrame) {
-               Jalview.currentAlignFrame = currentAlignFrame;
-       }
-}
+
+  public static Jalview getInstance()
+  {
+    return (Jalview) ApplicationSingletonProvider
+            .getInstance(Jalview.class);
+  }
+
+  private Jalview()
+  {
+  }
+
+  static
+  {
+    Platform.getURLCommandArguments();
+  }
+
+  private boolean headless;
+
+  public static boolean isHeadlessMode()
+  {
+    return getInstance().headless;
+  }
+
+  private Desktop desktop;
+
+  private AlignFrame currentAlignFrame;
+
+  public boolean isJavaAppletTag;
+
+  public String appletResourcePath;
+
+  JalviewAppLoader appLoader;
+
+  protected JSFunctionExec jsFunctionExec;
+
+  private boolean noCalculation, noMenuBar, noStatus;
+
+  private boolean noAnnotation;
+
+  public static final String TERMINATOR_LINE = "Jalview argument parsing complete.";
+
+  public boolean getStartCalculations()
+  {
+    return !noCalculation;
+  }
+
+  public boolean getAllowMenuBar()
+  {
+    return !noMenuBar;
+  }
+
+  public boolean getShowStatus()
+  {
+    return !noStatus;
+  }
+
+  public boolean getShowAnnotation()
+  {
+    return !noAnnotation;
+  }
+
+  public static AlignFrame getCurrentAlignFrame()
+  {
+    return getInstance().currentAlignFrame;
+  }
+
+  public static void setCurrentAlignFrame(AlignFrame currentAlignFrame)
+  {
+    getInstance().currentAlignFrame = currentAlignFrame;
+  }
+
+  static
+  {
+    if (!Platform.isJS())
+    /**
+     * Java only
+     * 
+     * @j2sIgnore
+     */
+    {
+      // grab all the rights we can for the JVM
+      Policy.setPolicy(new Policy()
+      {
+        @Override
+        public PermissionCollection getPermissions(CodeSource codesource)
+        {
+          Permissions perms = new Permissions();
+          perms.add(new AllPermission());
+          return (perms);
+        }
+
+        @Override
+        public void refresh()
+        {
+        }
+      });
+    }
+  }
+
+  /**
+   * keep track of feature fetching tasks.
+   * 
+   * @author JimP
+   * 
+   */
+  class FeatureFetcher
+  {
+    /*
+     * TODO: generalise to track all jalview events to orchestrate batch
+     * processing events.
+     */
+
+    private int queued = 0;
+
+    private int running = 0;
+
+    public FeatureFetcher()
+    {
+
+    }
+
+    public void addFetcher(final AlignFrame af,
+            final Vector<String> dasSources)
+    {
+      final long id = System.currentTimeMillis();
+      queued++;
+      final FeatureFetcher us = this;
+      new Thread(new Runnable()
+      {
+
+        @Override
+        public void run()
+        {
+          synchronized (us)
+          {
+            queued--;
+            running++;
+          }
+
+          af.setProgressBar(MessageManager
+                  .getString("status.das_features_being_retrived"), id);
+          af.featureSettings_actionPerformed(null);
+          af.setProgressBar(null, id);
+          synchronized (us)
+          {
+            running--;
+          }
+        }
+      }).start();
+    }
+
+    public synchronized boolean allFinished()
+    {
+      return queued == 0 && running == 0;
+    }
+
+  }
+
+  /**
+   * main class for Jalview application
+   * 
+   * @param args
+   *          open <em>filename</em>
+   */
+  public static void main(String[] args)
+  {
+    // Platform.startJavaLogging();
+    getInstance().doMain(args);
+  }
+
+
+  @SuppressWarnings("unused")
+  /**
+   * @param args
+   */
+  void doMain(String[] args)
+  {
+
+    boolean isJS = Platform.isJS();
+    if (isJS)
+    {
+      Platform.setAppClass(this);
+    }
+    else
+    {
+      System.setSecurityManager(null);
+    }
+
+    System.out
+            .println("Java version: " + System.getProperty("java.version"));
+    System.out.println(System.getProperty("os.arch") + " "
+            + System.getProperty("os.name") + " "
+            + System.getProperty("os.version"));
+
+    ArgsParser aparser = new ArgsParser(args);
+
+    String usrPropsFile = aparser.getValue(ArgsParser.PROPS);
+    Cache.loadProperties(usrPropsFile);
+
+    if (aparser.contains(ArgsParser.NODISPLAY)
+            || aparser.contains(ArgsParser.NOGUI)
+            || aparser.contains(ArgsParser.HEADLESS)
+            || "true".equals(System.getProperty("java.awt.headless")))
+    {
+      headless = true;
+      setSynchronous(true);
+    }
+
+    if (isJS)
+    {
+      isJavaAppletTag = aparser.isApplet();
+      if (isJavaAppletTag)
+      {
+        Preferences.setAppletDefaults();
+        Cache.loadProperties(usrPropsFile); // again, because we
+        // might be changing defaults here?
+      }
+      System.out.println(
+              "<Applet> found: " + aparser.getValue("Info.j2sAppletID"));
+      appletResourcePath = aparser.getValue("Info.resourcePath");
+    }
+    else
+    /**
+     * Java only
+     * 
+     * @j2sIgnore
+     */
+    {
+      if (usrPropsFile != null)
+      {
+        System.out.println(
+                "CMD [-props " + usrPropsFile + "] executed successfully!");
+      }
+
+      if (aparser.contains("help") || aparser.contains("h"))
+      {
+        showUsage();
+        System.exit(0);
+      }
+
+      // anything else!
+
+      final String jabawsUrl = aparser.getValue(ArgsParser.JABAWS);
+      if (jabawsUrl != null)
+      {
+        try
+        {
+          Jws2Discoverer.getInstance().setPreferredUrl(jabawsUrl);
+          System.out.println(
+                  "CMD [-jabaws " + jabawsUrl + "] executed successfully!");
+        } catch (MalformedURLException e)
+        {
+          System.err.println(
+                  "Invalid jabaws parameter: " + jabawsUrl + " ignored");
+        }
+      }
+
+    }
+    // check for property setting
+    String defs = aparser.getValue(ArgsParser.SETPROP);
+    while (defs != null)
+    {
+      int p = defs.indexOf('=');
+      if (p == -1)
+      {
+        System.err.println("Ignoring invalid setprop argument : " + defs);
+      }
+      else
+      {
+        System.out.println("Executing setprop argument: " + defs);
+        if (isJS)
+        {
+          Cache.setProperty(defs.substring(0, p), defs.substring(p + 1));
+        }
+      }
+      defs = aparser.getValue(ArgsParser.SETPROP);
+    }
+    System.setProperty("http.agent",
+            "Jalview Desktop/" + Cache.getDefault("VERSION", "Unknown"));
+    try
+    {
+      Cache.initLogger();
+    } catch (NoClassDefFoundError error)
+    {
+      error.printStackTrace();
+      System.out.println("\nEssential logging libraries not found."
+              + "\nUse: java -classpath \"$PATH_TO_LIB$/*:$PATH_TO_CLASSES$\" jalview.bin.Jalview");
+      System.exit(0);
+    }
+
+    desktop = null;
+
+    try
+    {
+      if (!isJS && Platform.isWin())
+      {
+        UIManager.setLookAndFeel(
+                headless ? "javax.swing.plaf.metal.MetalLookAndFeel"
+                        : UIManager.getSystemLookAndFeelClassName());
+        // UIManager.setLookAndFeel(UIManager.getSystemLookAndFeelClassName());
+      }
+    } catch (Exception ex)
+    {
+      System.err.println("Unexpected Look and Feel Exception");
+      ex.printStackTrace();
+    }
+    if (Platform.isAMacAndNotJS())
+    {
+
+      LookAndFeel lookAndFeel = ch.randelshofer.quaqua.QuaquaManager
+              .getLookAndFeel();
+      System.setProperty("com.apple.mrj.application.apple.menu.about.name",
+              "Jalview");
+      System.setProperty("apple.laf.useScreenMenuBar", "true");
+      if (lookAndFeel != null)
+      {
+        try
+        {
+          UIManager.setLookAndFeel(lookAndFeel);
+        } catch (Throwable e)
+        {
+          System.err.println(
+                  "Failed to set QuaQua look and feel: " + e.toString());
+        }
+      }
+      if (lookAndFeel == null
+              || !(lookAndFeel.getClass().isAssignableFrom(
+                      UIManager.getLookAndFeel().getClass()))
+              || !UIManager.getLookAndFeel().getClass().toString()
+                      .toLowerCase().contains("quaqua"))
+      {
+        try
+        {
+          System.err.println(
+                  "Quaqua LaF not available on this plaform. Using VAqua(4).\nSee https://issues.jalview.org/browse/JAL-2976");
+          UIManager.setLookAndFeel("org.violetlib.aqua.AquaLookAndFeel");
+        } catch (Throwable e)
+        {
+          System.err.println(
+                  "Failed to reset look and feel: " + e.toString());
+        }
+      }
+    }
+
+    /*
+     * configure 'full' SO model if preferences say to, 
+     * else use the default (SO Lite)
+     */
+    if (Cache.getDefault(Preferences.USE_FULL_SO, false))
+    {
+      SequenceOntologyFactory.setSequenceOntology(new SequenceOntology());
+    }
+
+    if (headless)
+    {
+      // If this is not tested, then
+
+      if (aparser.contains(ArgsParser.NOUSAGESTATS))
+      {
+        System.err.println("CMD [-nousagestats] executed successfully!");
+      }
+      if (aparser.contains(ArgsParser.NOQUESTIONNAIRE))
+      {
+        System.err.println("CMD [-noquestionnaire] executed successfully!");
+      }
+
+    }
+    else
+    {
+      desktop = Desktop.getInstance();
+      desktop.setInBatchMode(true); // indicate we are starting up
+      desktop.setVisible(true);
+
+      if (!isJS)
+      /**
+       * Java only
+       * 
+       * @j2sIgnore
+       */
+      {
+        desktop.startServiceDiscovery();
+        if (!aparser.contains(ArgsParser.NOUSAGESTATS))
+        {
+          startUsageStats(desktop);
+        }
+        else
+        {
+          System.err.println("CMD [-nousagestats] executed successfully!");
+        }
+
+        if (!aparser.contains(ArgsParser.NOQUESTIONNAIRE))
+        {
+          String url = aparser.getValue(ArgsParser.QUESTIONNAIRE);
+          if (url != null)
+          {
+            // Start the desktop questionnaire prompter with the specified
+            // questionnaire
+            Cache.log.debug("Starting questionnaire url at " + url);
+            desktop.checkForQuestionnaire(url);
+            System.out.println("CMD questionnaire[-" + url
+                    + "] executed successfully!");
+          }
+          else
+          {
+            if (Cache.getProperty(Preferences.NOQUESTIONNAIRES) == null)
+            {
+              // Start the desktop questionnaire prompter with the specified
+              // questionnaire
+              // String defurl =
+              // "http://anaplog.compbio.dundee.ac.uk/cgi-bin/questionnaire.pl";
+              // //
+              String defurl = "http://www.jalview.org/cgi-bin/questionnaire.pl";
+              Cache.log.debug(
+                      "Starting questionnaire with default url: " + defurl);
+              desktop.checkForQuestionnaire(defurl);
+            }
+          }
+        }
+        else
+        {
+          System.err
+                  .println("CMD [-noquestionnaire] executed successfully!");
+        }
+
+        if (!aparser.contains(ArgsParser.NONEWS))
+        {
+          desktop.checkForNews();
+        }
+
+        BioJsHTMLOutput.updateBioJS();
+      }
+    }
+
+    parseArguments(aparser, true);
+    System.err.println(TERMINATOR_LINE);
+  }
+
+  /**
+   * Allow an outside entity to initiate the second half of argument parsing
+   * (only).
+   * 
+   * @param args
+   * @return null is good
+   */
+  @Override
+  public Object parseArguments(String[] args)
+  {
+
+    try
+    {
+      ArgsParser aparser = new ArgsParser(args);
+      return parseArguments(aparser, false);
+    } catch (Throwable t)
+    {
+      return t;
+    }
+  }
+
+  /**
+   * 
+   * @param aparser
+   * @param isStartup
+   * @return
+   */
+  private Object parseArguments(ArgsParser aparser, boolean isStartup)
+  {
+    boolean isJS = Platform.isJS();
+
+    Desktop desktop = (headless ? null : Desktop.getInstance());
+    // script to execute after all loading is
+    // completed one way or another
+    // extract groovy argument and execute if necessary
+    String groovyscript = (isJS ? null
+            : aparser.getValue(ArgsParser.GROOVY, true));
+    String file = aparser.getValue(ArgsParser.OPEN, true);
+    // BH this here to allow split frame; not working as of 5/17/2019
+    String file2 = aparser.getValue(ArgsParser.OPEN2, true);
+    String fileFormat = (isJavaAppletTag
+            ? aparser.getAppletValue("format", null)
+            : null);
+    FileFormatI format = null;
+    DataSourceType protocol = null;
+
+    if (file == null && desktop == null)
+    {
+      System.out.println("No files to open!");
+      System.exit(1);
+    }
+    boolean haveImport = false;// checkStartVamas(aparser);
+    // Finally, deal with the remaining input data.
+    long progress = -1;
+    if (file == null && isJavaAppletTag)
+    {
+      // Maybe the sequences are added as parameters
+      StringBuffer data = new StringBuffer("PASTE");
+      int i = 1;
+      while ((file = aparser.getAppletValue("sequence" + i, null)) != null)
+      {
+        data.append(file.toString() + "\n");
+        i++;
+      }
+      if (data.length() > 5)
+      {
+        file = data.toString();
+      }
+    }
+
+    String data;
+
+    if (file != null)
+    {
+
+      if (!headless)
+      {
+        desktop.setProgressBar(
+                MessageManager
+                        .getString("status.processing_commandline_args"),
+                progress = System.currentTimeMillis());
+      }
+
+      if (!isJS)
+      /**
+       * ignore in JavaScript -- can't just check file existence - could load
+       * it?
+       * 
+       * @j2sIgnore
+       */
+      {
+        if (!file.startsWith("http://") && !file.startsWith("https://"))
+        // BH 2019 added https check for Java
+        {
+          if (!(new File(file)).exists())
+          {
+            System.out.println("Can't find " + file);
+            if (headless)
+            {
+              System.exit(1);
+            }
+          }
+        }
+      }
+
+      protocol = AppletFormatAdapter.checkProtocol(file);
+
+      try
+      {
+        format = (isJavaAppletTag && fileFormat != null
+                ? FileFormats.getInstance().forName(fileFormat)
+                : null);
+        if (format == null)
+        {
+          format = new IdentifyFile().identify(file, protocol);
+        }
+      } catch (FileFormatException e1)
+      {
+        // TODO ?
+      }
+
+      if (aparser.contains(ArgsParser.NOMENUBAR))
+      {
+        noMenuBar = true;
+        System.out.println("CMD [nomenu] executed successfully!");
+      }
+
+      if (aparser.contains(ArgsParser.NOSTATUS))
+      {
+        noStatus = true;
+        System.out.println("CMD [nostatus] executed successfully!");
+      }
+
+      if (aparser.contains(ArgsParser.NOANNOTATION)
+              || aparser.contains(ArgsParser.NOANNOTATION2))
+      {
+        noAnnotation = true;
+        System.out.println("CMD no-annotation executed successfully!");
+      }
+      if (aparser.contains(ArgsParser.NOCALCULATION))
+      {
+        noCalculation = true;
+        System.out.println("CMD [nocalculation] executed successfully!");
+      }
+
+      AlignFrame af = new FileLoader(!headless).LoadFileWaitTillLoaded(file,
+              protocol, format);
+      if (af == null)
+      {
+        System.out.println("error");
+      }
+      else
+      {
+        System.out
+                .println("CMD [-open " + file + "] executed successfully!");
+        if (file2 != null)
+        {
+          protocol = AppletFormatAdapter.checkProtocol(file2);
+          try
+          {
+            format = new IdentifyFile().identify(file2, protocol);
+          } catch (FileFormatException e1)
+          {
+            // TODO ?
+          }
+          AlignFrame af2 = new FileLoader(!headless)
+                  .LoadFileWaitTillLoaded(file2, protocol, format);
+          if (af2 == null)
+          {
+            System.out.println("error");
+          }
+          else
+          {
+            AlignViewport.openLinkedAlignmentAs(af,
+                    af.getViewport().getAlignment(),
+                    af2.getViewport().getAlignment(), "",
+                    AlignViewport.SPLIT_FRAME);
+            System.out.println(
+                    "CMD [-open2 " + file2 + "] executed successfully!");
+          }
+        }
+
+        setCurrentAlignFrame(af);
+
+        // TODO: file2 How to implement file2 for the applet spit screen?
+
+        data = aparser.getValue(ArgsParser.COLOUR, true);
+        if (data != null)
+        {
+          data.replaceAll("%20", " ");
+
+          ColourSchemeI cs = ColourSchemeProperty.getColourScheme(
+                  af.getViewport(), af.getViewport().getAlignment(), data);
+
+          if (cs != null)
+          {
+            System.out.println(
+                    "CMD [-color " + data + "] executed successfully!");
+          }
+          af.changeColour(cs);
+        }
+
+        // Must maintain ability to use the groups flag
+        data = aparser.getValue(ArgsParser.GROUPS, true);
+        if (data != null)
+        {
+          af.parseFeaturesFile(data,
+                  AppletFormatAdapter.checkProtocol(data));
+          // System.out.println("Added " + data);
+          System.out.println(
+                  "CMD groups[-" + data + "]  executed successfully!");
+        }
+        data = aparser.getValue(ArgsParser.FEATURES, true);
+        if (data != null)
+        {
+          af.parseFeaturesFile(data,
+                  AppletFormatAdapter.checkProtocol(data));
+          // System.out.println("Added " + data);
+          System.out.println(
+                  "CMD [-features " + data + "]  executed successfully!");
+        }
+
+        data = aparser.getValue(ArgsParser.ANNOTATIONS, true);
+        if (data != null)
+        {
+          af.loadJalviewDataFile(data, null, null, null);
+          // System.out.println("Added " + data);
+          System.out.println(
+                  "CMD [-annotations " + data + "] executed successfully!");
+        }
+
+        if (aparser.contains(ArgsParser.SHOWOVERVIEW))
+        {
+          af.overviewMenuItem_actionPerformed(null);
+          System.out.println("CMD [showoverview] executed successfully!");
+        }
+
+        // set or clear the sortbytree flag.
+        if (aparser.contains(ArgsParser.SORTBYTREE))
+        {
+          af.getViewport().setSortByTree(true);
+          if (af.getViewport().getSortByTree())
+          {
+            System.out.println("CMD [-sortbytree] executed successfully!");
+          }
+        }
+
+        boolean doUpdateAnnotation = false;
+
+        /**
+         * we do this earlier in JalviewJS because of a complication with
+         * SHOWOVERVIEW
+         * 
+         * For now, just fixing this in JalviewJS.
+         *
+         * 
+         * @j2sIgnore
+         * 
+         */
+        {
+          if (aparser.contains(ArgsParser.NOANNOTATION)
+                  || aparser.contains(ArgsParser.NOANNOTATION2))
+          {
+            af.getViewport().setShowAnnotation(false);
+            if (!af.getViewport().isShowAnnotation())
+            {
+              doUpdateAnnotation = true;
+              System.out
+                      .println("CMD no-annotation executed successfully!");
+            }
+          }
+        }
+        if (aparser.contains(ArgsParser.NOSORTBYTREE))
+        {
+          af.getViewport().setSortByTree(false);
+          if (!af.getViewport().getSortByTree())
+          {
+            doUpdateAnnotation = true;
+            System.out
+                    .println("CMD [-nosortbytree] executed successfully!");
+          }
+        }
+        if (doUpdateAnnotation)
+        { // BH 2019.07.24
+          af.setMenusForViewport();
+          af.alignPanel.updateLayout();
+        }
+        data = aparser.getValue(ArgsParser.TREE, true);
+        if (data != null)
+        {
+          try
+          {
+            System.out.println(
+                    "CMD [-tree " + data + "] executed successfully!");
+            NewickFile nf = new NewickFile(data,
+                    AppletFormatAdapter.checkProtocol(data));
+            af.getViewport()
+                    .setCurrentTree(af.showNewickTree(nf, data).getTree());
+          } catch (IOException ex)
+          {
+            System.err.println("Couldn't add tree " + data);
+            ex.printStackTrace(System.err);
+          }
+        }
+        // TODO - load PDB structure(s) to alignment JAL-629
+        // (associate with identical sequence in alignment, or a specified
+        // sequence)
+        if (isJavaAppletTag)
+        {
+          loadAppletParams(aparser, af);
+        }
+        else if (!isJS)
+        /**
+         * Java only
+         * 
+         * @j2sIgnore
+         */
+        {
+          if (groovyscript != null)
+          {
+            // Execute the groovy script after we've done all the rendering
+            // stuff
+            // and before any images or figures are generated.
+            System.out.println("Executing script " + groovyscript);
+            executeGroovyScript(groovyscript, af);
+            System.out.println("CMD groovy[" + groovyscript
+                    + "] executed successfully!");
+            groovyscript = null;
+          }
+        }
+        createOutputFiles(aparser, af, format);
+        while (aparser.getSize() > 0)
+        {
+          System.out.println("Unknown arg: " + aparser.nextValue());
+        }
+      }
+    }
+    AlignFrame startUpAlframe = null;
+    // We'll only open the default file if the desktop is visible.
+    // And the user
+    // ////////////////////
+
+    if (!isJS && !headless && file == null && !haveImport
+            && jalview.bin.Cache.getDefault("SHOW_STARTUP_FILE", true))
+    /**
+     * Java only
+     * 
+     * @j2sIgnore
+     */
+    {
+      file = jalview.bin.Cache.getDefault("STARTUP_FILE",
+              jalview.bin.Cache.getDefault("www.jalview.org",
+                      "http://www.jalview.org")
+                      + "/examples/exampleFile_2_7.jar");
+      if (file.equals(
+              "http://www.jalview.org/examples/exampleFile_2_3.jar"))
+      {
+        // hardwire upgrade of the startup file
+        file.replace("_2_3.jar", "_2_7.jar");
+        // and remove the stale setting
+        jalview.bin.Cache.removeProperty("STARTUP_FILE");
+      }
+
+      protocol = DataSourceType.FILE;
+
+      if (file.indexOf("http:") > -1)
+      {
+        protocol = DataSourceType.URL;
+      }
+
+      if (file.endsWith(".jar"))
+      {
+        format = FileFormat.Jalview;
+      }
+      else
+      {
+        try
+        {
+          format = new IdentifyFile().identify(file, protocol);
+        } catch (FileFormatException e)
+        {
+          // TODO what?
+        }
+      }
+
+      startUpAlframe = new FileLoader(!headless)
+              .LoadFileWaitTillLoaded(file, protocol, format);
+      // extract groovy arguments before anything else.
+    }
+
+    // Once all other stuff is done, execute any groovy scripts (in order)
+    if (groovyscript != null)
+    {
+      if (Cache.groovyJarsPresent())
+      {
+        System.out.println("Executing script " + groovyscript);
+        executeGroovyScript(groovyscript, startUpAlframe);
+      }
+      else
+      {
+        System.err.println(
+                "Sorry. Groovy Support is not available, so ignoring the provided groovy script "
+                        + groovyscript);
+      }
+    }
+    // and finally, turn off batch mode indicator - if the desktop still exists
+    if (desktop != null)
+    {
+      if (progress != -1)
+      {
+        desktop.setProgressBar(null, progress);
+      }
+      desktop.setInBatchMode(false);
+    }
+
+    return null;
+  }
+
+  /**
+   * Writes an output file for each format (if any) specified in the
+   * command-line arguments. Supported formats are currently
+   * <ul>
+   * <li>png</li>
+   * <li>svg</li>
+   * <li>html</li>
+   * <li>biojsmsa</li>
+   * <li>imgMap</li>
+   * <li>eps</li>
+   * </ul>
+   * A format parameter should be followed by a parameter specifying the output
+   * file name. {@code imgMap} parameters should follow those for the
+   * corresponding alignment image output.
+   * 
+   * @param aparser
+   * @param af
+   * @param format
+   */
+  private void createOutputFiles(ArgsParser aparser, AlignFrame af,
+          FileFormatI format)
+  {
+    String imageName = "unnamed.png";
+    while (aparser.getSize() > 1)
+    {
+      String outputFormat = aparser.nextValue();
+      String file = aparser.nextValue();
+      // System.out.println("format " + outputFormat);
+
+      if (outputFormat.equalsIgnoreCase("png"))
+      {
+        af.createPNG(new File(file));
+        imageName = (new File(file)).getName();
+        System.out.println("Creating PNG image: " + file);
+        continue;
+      }
+      else if (outputFormat.equalsIgnoreCase("svg"))
+      {
+        File imageFile = new File(file);
+        imageName = imageFile.getName();
+        af.createSVG(imageFile);
+        System.out.println("Creating SVG image: " + file);
+        continue;
+      }
+      else if (outputFormat.equalsIgnoreCase("html"))
+      {
+        File imageFile = new File(file);
+        imageName = imageFile.getName();
+        HtmlSvgOutput htmlSVG = new HtmlSvgOutput(af.alignPanel);
+        htmlSVG.exportHTML(file);
+
+        System.out.println("Creating HTML image: " + file);
+        continue;
+      }
+      else if (outputFormat.equalsIgnoreCase("biojsmsa"))
+      {
+        if (file == null)
+        {
+          System.err.println("The output html file must not be null");
+          return;
+        }
+        try
+        {
+          BioJsHTMLOutput.refreshVersionInfo(
+                  BioJsHTMLOutput.BJS_TEMPLATES_LOCAL_DIRECTORY);
+        } catch (URISyntaxException e)
+        {
+          e.printStackTrace();
+        }
+        BioJsHTMLOutput bjs = new BioJsHTMLOutput(af.alignPanel);
+        bjs.exportHTML(file);
+        System.out.println("Creating BioJS MSA Viwer HTML file: " + file);
+        continue;
+      }
+      else if (outputFormat.equalsIgnoreCase("imgMap"))
+      {
+        af.createImageMap(new File(file), imageName);
+        System.out.println("Creating image map: " + file);
+        continue;
+      }
+      else if (outputFormat.equalsIgnoreCase("eps"))
+      {
+        File outputFile = new File(file);
+        System.out.println(
+                "Creating EPS file: " + outputFile.getAbsolutePath());
+        af.createEPS(outputFile);
+        continue;
+      }
+
+      af.saveAlignment(file, format);
+      if (af.isSaveAlignmentSuccessful())
+      {
+        System.out.println(
+                "Written alignment in " + format + " format to " + file);
+      }
+      else
+      {
+        System.out.println("Error writing file " + file + " in " + format
+                + " format!!");
+      }
+
+    }
+  }
+
+  private static void showUsage()
+  {
+    System.out.println(
+            "Usage: jalview -open [FILE] [OUTPUT_FORMAT] [OUTPUT_FILE]\n\n"
+                    + "-nodisplay\tRun Jalview without User Interface.\n"
+                    + "-props FILE\tUse the given Jalview properties file instead of users default.\n"
+                    + "-colour COLOURSCHEME\tThe colourscheme to be applied to the alignment\n"
+                    + "-annotations FILE\tAdd precalculated annotations to the alignment.\n"
+                    + "-tree FILE\tLoad the given newick format tree file onto the alignment\n"
+                    + "-features FILE\tUse the given file to mark features on the alignment.\n"
+                    + "-fasta FILE\tCreate alignment file FILE in Fasta format.\n"
+                    + "-clustal FILE\tCreate alignment file FILE in Clustal format.\n"
+                    + "-pfam FILE\tCreate alignment file FILE in PFAM format.\n"
+                    + "-msf FILE\tCreate alignment file FILE in MSF format.\n"
+                    + "-pileup FILE\tCreate alignment file FILE in Pileup format\n"
+                    + "-pir FILE\tCreate alignment file FILE in PIR format.\n"
+                    + "-blc FILE\tCreate alignment file FILE in BLC format.\n"
+                    + "-json FILE\tCreate alignment file FILE in JSON format.\n"
+                    + "-jalview FILE\tCreate alignment file FILE in Jalview format.\n"
+                    + "-png FILE\tCreate PNG image FILE from alignment.\n"
+                    + "-svg FILE\tCreate SVG image FILE from alignment.\n"
+                    + "-html FILE\tCreate HTML file from alignment.\n"
+                    + "-biojsMSA FILE\tCreate BioJS MSA Viewer HTML file from alignment.\n"
+                    + "-imgMap FILE\tCreate HTML file FILE with image map of PNG image.\n"
+                    + "-eps FILE\tCreate EPS file FILE from alignment.\n"
+                    + "-questionnaire URL\tQueries the given URL for information about any Jalview user questionnaires.\n"
+                    + "-noquestionnaire\tTurn off questionnaire check.\n"
+                    + "-nonews\tTurn off check for Jalview news.\n"
+                    + "-nousagestats\tTurn off google analytics tracking for this session.\n"
+                    + "-sortbytree OR -nosortbytree\tEnable or disable sorting of the given alignment by the given tree\n"
+                    // +
+                    // "-setprop PROPERTY=VALUE\tSet the given Jalview property,
+                    // after all other properties files have been read\n\t
+                    // (quote the 'PROPERTY=VALUE' pair to ensure spaces are
+                    // passed in correctly)"
+                    + "-jabaws URL\tSpecify URL for Jabaws services (e.g. for a local installation).\n"
+                    + "-fetchfrom nickname\tQuery nickname for features for the alignments and display them.\n"
+                    // +
+                    // "-vdoc vamsas-document\tImport vamsas document into new
+                    // session or join existing session with same URN\n"
+                    // + "-vses vamsas-session\tJoin session with given URN\n"
+                    + "-groovy FILE\tExecute groovy script in FILE, after all other arguments have been processed (if FILE is the text 'STDIN' then the file will be read from STDIN)\n"
+                    + "\n~Read documentation in Application or visit http://www.jalview.org for description of Features and Annotations file~\n\n");
+  }
+
+  private static void startUsageStats(final Desktop desktop)
+  {
+    /**
+     * start a User Config prompt asking if we can log usage statistics.
+     */
+    PromptUserConfig prompter = new PromptUserConfig(
+            Desktop.getDesktopPane(), "USAGESTATS",
+            "Jalview Usage Statistics",
+            "Do you want to help make Jalview better by enabling "
+                    + "the collection of usage statistics with Google Analytics ?"
+                    + "\n\n(you can enable or disable usage tracking in the preferences)",
+            new Runnable()
+            {
+              @Override
+              public void run()
+              {
+                Cache.log.debug(
+                        "Initialising googletracker for usage stats.");
+                Cache.initGoogleTracker();
+                Cache.log.debug("Tracking enabled.");
+              }
+            }, new Runnable()
+            {
+              @Override
+              public void run()
+              {
+                Cache.log.debug("Not enabling Google Tracking.");
+              }
+            }, null, true);
+    desktop.addDialogThread(prompter);
+  }
+
+  /**
+   * Locate the given string as a file and pass it to the groovy interpreter.
+   * 
+   * @param groovyscript
+   *          the script to execute
+   * @param jalviewContext
+   *          the Jalview Desktop object passed in to the groovy binding as the
+   *          'Jalview' object.
+   */
+  private void executeGroovyScript(String groovyscript, AlignFrame af)
+  {
+    /**
+     * for scripts contained in files
+     */
+    File tfile = null;
+    /**
+     * script's URI
+     */
+    URL sfile = null;
+    if (groovyscript.trim().equals("STDIN"))
+    {
+      // read from stdin into a tempfile and execute it
+      try
+      {
+        tfile = File.createTempFile("jalview", "groovy");
+        PrintWriter outfile = new PrintWriter(
+                new OutputStreamWriter(new FileOutputStream(tfile)));
+        BufferedReader br = new BufferedReader(
+                new InputStreamReader(System.in));
+        String line = null;
+        while ((line = br.readLine()) != null)
+        {
+          outfile.write(line + "\n");
+        }
+        br.close();
+        outfile.flush();
+        outfile.close();
+
+      } catch (Exception ex)
+      {
+        System.err.println("Failed to read from STDIN into tempfile "
+                + ((tfile == null) ? "(tempfile wasn't created)"
+                        : tfile.toString()));
+        ex.printStackTrace();
+        return;
+      }
+      try
+      {
+        sfile = tfile.toURI().toURL();
+      } catch (Exception x)
+      {
+        System.err.println(
+                "Unexpected Malformed URL Exception for temporary file created from STDIN: "
+                        + tfile.toURI());
+        x.printStackTrace();
+        return;
+      }
+    }
+    else
+    {
+      try
+      {
+        sfile = new URI(groovyscript).toURL();
+      } catch (Exception x)
+      {
+        tfile = new File(groovyscript);
+        if (!tfile.exists())
+        {
+          System.err.println("File '" + groovyscript + "' does not exist.");
+          return;
+        }
+        if (!tfile.canRead())
+        {
+          System.err.println("File '" + groovyscript + "' cannot be read.");
+          return;
+        }
+        if (tfile.length() < 1)
+        {
+          System.err.println("File '" + groovyscript + "' is empty.");
+          return;
+        }
+        try
+        {
+          sfile = tfile.getAbsoluteFile().toURI().toURL();
+        } catch (Exception ex)
+        {
+          System.err.println("Failed to create a file URL for "
+                  + tfile.getAbsoluteFile());
+          return;
+        }
+      }
+    }
+    try
+    {
+      Map<String, Object> vbinding = new HashMap<>();
+      vbinding.put("Jalview", this);
+      if (af != null)
+      {
+        vbinding.put("currentAlFrame", af);
+      }
+      Binding gbinding = new Binding(vbinding);
+      GroovyScriptEngine gse = new GroovyScriptEngine(new URL[] { sfile });
+      gse.run(sfile.toString(), gbinding);
+      if ("STDIN".equals(groovyscript))
+      {
+        // delete temp file that we made -
+        // only if it was successfully executed
+        tfile.delete();
+      }
+    } catch (Exception e)
+    {
+      System.err.println("Exception Whilst trying to execute file " + sfile
+              + " as a groovy script.");
+      e.printStackTrace(System.err);
+
+    }
+  }
+
+  public AlignFrame[] getAlignFrames()
+  {
+    return desktop == null ? new AlignFrame[] { getCurrentAlignFrame() }
+            : Desktop.getAlignFrames();
+
+  }
+
+  /**
+   * Quit method delegates to Desktop.quit - unless running in headless mode
+   * when it just ends the JVM
+   */
+  public void quit()
+  {
+    if (jsFunctionExec != null)
+    {
+      jsFunctionExec.tidyUp();
+      jsFunctionExec = null;
+    }
+
+    if (desktop != null)
+    {
+      desktop.quit();
+    }
+    else
+    {
+      System.exit(0);
+    }
+  }
+
+  /**
+   * Handle all JalviewLite applet parameters
+   * 
+   * @param aparser
+   * @param af
+   */
+  private void loadAppletParams(ArgsParser aparser, AlignFrame af)
+  {
+    JalviewApp app = new JalviewApp()
+    {
+
+      // TODO BH 2019
+      //
+      // These are methods that are in JalviewLite that various classes call
+      // but are not in JalviewLiteJsApi. Or, even if they are, other classes
+      // call
+      // them to JalviewLite directly. Some may not be necessary, but they have
+      // to
+      // be at least mentioned here, or the classes calling them should
+      // reference
+      // JalviewLite itself.
+
+      private boolean alignPDBStructures; // From JalviewLite; not implemented
+
+      private Hashtable<String, Hashtable<String, String[]>> jsmessages;
+
+      private Hashtable<String, int[]> jshashes;
+
+      @Override
+      public String getParameter(String name)
+      {
+        return aparser.getAppletValue(name, null);
+      }
+
+      @Override
+      public boolean getDefaultParameter(String name, boolean def)
+      {
+        String stn;
+        return ((stn = getParameter(name)) == null ? def
+                : "true".equalsIgnoreCase(stn));
+      }
+
+      /**
+       * Get the applet-like document base even though this is an application.
+       */
+      @Override
+      public URL getDocumentBase()
+      {
+        return Platform.getDocumentBase();
+      }
+
+      /**
+       * Get the applet-like code base even though this is an application.
+       */
+      @Override
+      public URL getCodeBase()
+      {
+        return Platform.getCodeBase();
+      }
+
+      @Override
+      public AlignViewportI getViewport()
+      {
+        return af.getViewport();
+      }
+
+      /**
+       * features
+       * 
+       */
+      @Override
+      public boolean parseFeaturesFile(String filename,
+              DataSourceType protocol)
+      {
+        return af.parseFeaturesFile(filename, protocol);
+      }
+
+      /**
+       * scorefile
+       * 
+       */
+      @Override
+      public boolean loadScoreFile(String sScoreFile) throws IOException
+      {
+        af.loadJalviewDataFile(sScoreFile, null, null, null);
+        return true;
+      }
+
+      /**
+       * annotations, jpredfile, jnetfile
+       * 
+       */
+      @Override
+      public void updateForAnnotations()
+      {
+        af.updateForAnnotations();
+      }
+
+      @Override
+      public void loadTree(NewickFile fin, String treeFile)
+              throws IOException
+      {
+        // n/a -- already done by standard Jalview command line processing
+      }
+
+      @Override
+      public void setAlignPdbStructures(boolean defaultParameter)
+      {
+        alignPDBStructures = true;
+      }
+
+      @Override
+      public void newStructureView(PDBEntry pdb, SequenceI[] seqs,
+              String[] chains, DataSourceType protocol)
+      {
+        StructureViewer.launchStructureViewer(af.alignPanel, pdb, seqs);
+      }
+
+      @Override
+      public void setFeatureGroupState(String[] groups, boolean state)
+      {
+        af.setFeatureGroupState(groups, state);
+      }
+
+      @Override
+      public void alignedStructureView(PDBEntry[] pdb, SequenceI[][] seqs,
+              String[][] chains, String[] protocols)
+      {
+        System.err.println(
+                "Jalview applet interface alignedStructureView not implemented");
+      }
+
+      @Override
+      public void newFeatureSettings()
+      {
+        System.err.println(
+                "Jalview applet interface newFeatureSettings not implemented");
+      }
+
+      private Vector<Runnable> jsExecQueue;
+
+      @Override
+      public Vector<Runnable> getJsExecQueue(JSFunctionExec exec)
+      {
+        jsFunctionExec = exec;
+        return (jsExecQueue == null ? (jsExecQueue = new Vector<>())
+                : jsExecQueue);
+      }
+
+      @Override
+      public AppletContext getAppletContext()
+      {
+        // TODO Auto-generated method stub
+        return null;
+      }
+
+      @Override
+      public boolean isJsfallbackEnabled()
+      {
+        // TODO Auto-generated method stub
+        return false;
+      }
+
+      @Override
+      public JSObject getJSObject()
+      {
+        // TODO Auto-generated method stub
+        return null;
+      }
+
+      @Override
+      public StructureSelectionManagerProvider getStructureSelectionManagerProvider()
+      {
+        // TODO Q: what exactly is this? BH
+        return null;
+      }
+
+      @Override
+      public void updateColoursFromMouseOver(Object source,
+              MouseOverStructureListener mouseOverStructureListener)
+      {
+        // TODO Auto-generated method stub
+
+      }
+
+      @Override
+      public Object[] getSelectionForListener(SequenceGroup seqsel,
+              ColumnSelection colsel, HiddenColumns hidden,
+              SelectionSource source, Object alignFrame)
+      {
+        return appLoader.getSelectionForListener(getCurrentAlignFrame(),
+                seqsel, colsel, hidden, source, alignFrame);
+      }
+
+      @Override
+      public String arrayToSeparatorList(String[] array)
+      {
+        return appLoader.arrayToSeparatorList(array);
+      }
+
+      @Override
+      public Hashtable<String, int[]> getJSHashes()
+      {
+        return (jshashes == null ? (jshashes = new Hashtable<>())
+                : jshashes);
+      }
+
+      @Override
+      public Hashtable<String, Hashtable<String, String[]>> getJSMessages()
+      {
+        return (jsmessages == null ? (jsmessages = new Hashtable<>())
+                : jsmessages);
+      }
+
+      @Override
+      public Object getFrameForSource(VamsasSource source)
+      {
+        if (source != null)
+        {
+          AlignFrame af;
+          if (source instanceof jalview.gui.AlignViewport
+                  && source == (af = getCurrentAlignFrame()).getViewport())
+          {
+            // should be valid if it just generated an event!
+            return af;
+          }
+          // TODO: ensure that if '_af' is specified along with a handler
+          // function, then only events from that alignFrame are sent to that
+          // function
+        }
+        return null;
+      }
+
+      @Override
+      public FeatureRenderer getNewFeatureRenderer(AlignViewportI vp)
+      {
+        return new jalview.gui.FeatureRenderer((AlignmentPanel) vp);
+      }
+
+    };
+
+    appLoader = new JalviewAppLoader(true);
+    appLoader.load(app);
+  }
+
+  /**
+   * 
+   * @see jalview.bin.JalviewLiteJsApi#getSelectedSequences()
+   */
+  @Override
+  public String getSelectedSequences()
+  {
+    return getSelectedSequencesFrom(getCurrentAlignFrame());
+  }
+
+  /**
+   * 
+   * @see jalview.bin.JalviewLiteJsApi#getSelectedSequences(java.lang.String)
+   */
+  @Override
+  public String getSelectedSequences(String sep)
+  {
+    return getSelectedSequencesFrom(getCurrentAlignFrame(), sep);
+  }
+
+  /**
+   * 
+   * @see jalview.bin.JalviewLiteJsApi#getSelectedSequencesFrom(jalview.appletgui
+   *      .AlignFrame)
+   */
+  @Override
+  public String getSelectedSequencesFrom(AlignFrameI alf)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return getSelectedSequencesFrom(alf, null);
+  }
+
+  /**
+   * 
+   * @see jalview.bin.JalviewLiteJsApi#getSelectedSequencesFrom(jalview.appletgui
+   *      .AlignFrame, java.lang.String)
+   */
+  @Override
+  public String getSelectedSequencesFrom(AlignFrameI alf, String sep)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.getSelectedSequencesFrom(alf, sep);
+  }
+
+  /**
+   * 
+   * @see jalview.bin.JalviewLiteJsApi#getSelectedSequencesFrom(jalview.appletgui
+   *      .AlignFrame, java.lang.String)
+   */
+  @Override
+  public void highlight(String sequenceId, String position,
+          String alignedPosition)
+  {
+    highlightIn(null, sequenceId, position,
+            alignedPosition);
+  }
+
+  @Override
+  public void highlightIn(AlignFrameI alf, String sequenceId,
+          String position, String alignedPosition)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    appLoader.highlightIn(alf, sequenceId, position, alignedPosition);
+  }
+
+  @Override
+  public void select(String sequenceIds, String columns)
+  {
+    selectIn(getCurrentAlignFrame(), sequenceIds, columns, null);
+  }
+
+  @Override
+  public void select(String sequenceIds, String columns, String sep)
+  {
+    selectIn(null, sequenceIds, columns, sep);
+  }
+
+  @Override
+  public void selectIn(AlignFrameI alf, String sequenceIds, String columns)
+  {
+    selectIn(alf, sequenceIds, columns, null);
+  }
+
+  @Override
+  public void selectIn(AlignFrameI alf, String sequenceIds, String columns,
+          String sep)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    appLoader.selectIn(alf, sequenceIds, columns, sep);
+  }
+
+  @Override
+  public String getSelectedSequencesAsAlignment(String format,
+          String suffix)
+  {
+    return getSelectedSequencesAsAlignmentFrom(null,
+            format, suffix);
+  }
+
+  @Override
+  public String getSelectedSequencesAsAlignmentFrom(AlignFrameI alf,
+          String format, String sep)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.getSelectedSequencesAsAlignmentFrom(alf, format, sep);
+  }
+
+  @Override
+  public String getAlignmentOrder()
+  {
+    return getAlignmentFrom(getCurrentAlignFrame(), null);
+  }
+
+  @Override
+  public String getAlignmentOrderFrom(AlignFrameI alf)
+  {
+    return getAlignmentFrom(alf, null);
+  }
+
+  @Override
+  public String getAlignmentOrderFrom(AlignFrameI alf, String sep)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.getAlignmentOrderFrom(alf, sep);
+  }
+
+  @Override
+  public String orderBy(String order, String undoName)
+  {
+    return orderBy(order, undoName, null);
+  }
+
+  @Override
+  public String orderBy(String order, String undoName, String sep)
+  {
+    return orderAlignmentBy(getCurrentAlignFrame(), order, undoName, sep);
+  }
+
+  @Override
+  public String orderAlignmentBy(AlignFrameI alf, String order,
+          String undoName, String sep)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.orderAlignmentBy(alf, order, undoName, sep);
+  }
+
+  @Override
+  public String getAlignment(String format)
+  {
+    return getAlignmentFrom(null, format, null);
+  }
+
+  @Override
+  public String getAlignmentFrom(AlignFrameI alf, String format)
+  {
+    return getAlignmentFrom(alf, format, null);
+  }
+
+  @Override
+  public String getAlignment(String format, String suffix)
+  {
+    return getAlignmentFrom(getCurrentAlignFrame(), format, suffix);
+  }
+
+  @Override
+  public String getAlignmentFrom(AlignFrameI alf, String format,
+          String suffix)
+  {
+    return appLoader.getAlignmentFrom(alf, format, suffix);
+  }
+
+  @Override
+  public void loadAnnotation(String annotation)
+  {
+    loadAnnotationFrom(getCurrentAlignFrame(), annotation);
+  }
+
+  @Override
+  public void loadAnnotationFrom(AlignFrameI alf, String annotation)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    appLoader.loadAnnotationFrom(alf, annotation);
+  }
+
+  @Override
+  public void loadFeatures(String features, boolean autoenabledisplay)
+  {
+    loadFeaturesFrom(currentAlignFrame, features, autoenabledisplay);
+  }
+
+  @Override
+  public boolean loadFeaturesFrom(AlignFrameI alf, String features,
+          boolean autoenabledisplay)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.loadFeaturesFrom(alf, features, autoenabledisplay);
+  }
+
+  @Override
+  public String getFeatures(String format)
+  {
+    return getFeaturesFrom(null, format);
+  }
+
+  @Override
+  public String getFeaturesFrom(AlignFrameI alf, String format)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.getFeaturesFrom(alf, format, true, false);
+  }
+
+  @Override
+  public String getAnnotation()
+  {
+    return getAnnotationFrom(null);
+  }
+
+  @Override
+  public String getAnnotationFrom(AlignFrameI alf)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.getAnnotationFrom(alf);
+  }
+
+  @Override
+  public AlignFrameI newView()
+  {
+    return newViewFrom(null, null);
+  }
+
+  @Override
+  public AlignFrameI newView(String name)
+  {
+    return newViewFrom(null, name);
+  }
+
+  @Override
+  public AlignFrameI newViewFrom(AlignFrameI alf)
+  {
+    return newViewFrom(alf, null);
+  }
+
+  @Override
+  public AlignFrameI newViewFrom(AlignFrameI alf, String name)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.newViewFrom(alf, name);
+  }
+
+  @Override
+  public AlignFrameI loadAlignment(String text, String title)
+  {
+    return appLoader.loadAlignment(text, AlignFrame.DEFAULT_WIDTH,
+            AlignFrame.DEFAULT_HEIGHT, title);
+  }
+
+  @Override
+  public boolean addPdbFile(AlignFrameI alFrame, String sequenceId,
+          String pdbEntryString, String pdbFile)
+  {
+    if (alFrame == null)
+    {
+      alFrame = getCurrentAlignFrame();
+    }
+    return appLoader.addPdbFile(alFrame, sequenceId, pdbEntryString,
+            pdbFile);
+  }
+
+  @Override
+  public void scrollViewToIn(AlignFrameI alf, String topRow,
+          String leftHandColumn)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    appLoader.scrollViewToIn(alf, topRow, leftHandColumn);
+  }
+
+  @Override
+  public void scrollViewToRowIn(AlignFrameI alf, String topRow)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    appLoader.scrollViewToRowIn(alf, topRow);
+  }
+
+  @Override
+  public void scrollViewToColumnIn(AlignFrameI alf, String leftHandColumn)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    appLoader.scrollViewToColumnIn(alf, leftHandColumn);
+  }
+
+  @Override
+  public String getFeatureGroups()
+  {
+    return getFeatureGroupsOn(null);
+  }
+
+  @Override
+  public String getFeatureGroupsOn(AlignFrameI alf)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.getFeatureGroupsOn(alf);
+  }
+
+  @Override
+  public String getFeatureGroupsOfState(boolean visible)
+  {
+    return getFeatureGroupsOfStateOn(null, visible);
+  }
+
+  @Override
+  public String getFeatureGroupsOfStateOn(AlignFrameI alf, boolean visible)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.getFeatureGroupsOfStateOn(alf, visible);
+  }
+
+  @Override
+  public void setFeatureGroupState(String groups, boolean state)
+  { // JalviewLite API
+    setFeatureGroupStateOn(null, groups, state);
+  }
+
+  @Override
+  public void setFeatureGroupStateOn(AlignFrameI alf, String groups,
+          boolean state)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    appLoader.setFeatureGroupStateOn(alf, groups, state);
+  }
+
+  @Override
+  public String getSeparator()
+  {
+    return appLoader.getSeparator();
+  }
+
+  @Override
+  public void setSeparator(String separator)
+  {
+    appLoader.setSeparator(separator);
+  }
+
+  @Override
+  public String getJsMessage(String messageclass, String viewId)
+  {
+    // see http://www.jalview.org/examples/jalviewLiteJs.html
+    return null;
+  }
+
+  /**
+   * Open a new Tree panel on the desktop statically. Params are standard (not
+   * set by Groovy). No dialog is opened.
+   * 
+   * @param af
+   * @param treeType
+   * @param modelName
+   * @return null, or the string "label.you_need_at_least_n_sequences" if number
+   *         of sequences selected is inappropriate
+   */
+  @Override
+  public Object openTreePanel(AlignFrame af, String treeType,
+          String modelName)
+  { // JalviewJS api
+    if (af == null)
+    {
+      af = getCurrentAlignFrame();
+    }
+    return CalculationChooser.openTreePanel(af, treeType, modelName, null);
+  }
+
+  /**
+   * public static method for JalviewJS API to open a PCAPanel without
+   * necessarily using a dialog.
+   * 
+   * @param af
+   * @param modelName
+   * @return the PCAPanel, or the string "label.you_need_at_least_n_sequences"
+   *         if number of sequences selected is inappropriate
+   */
+  @Override
+  public Object openPcaPanel(AlignFrame af, String modelName)
+  {
+    if (af == null)
+    {
+      af = getCurrentAlignFrame();
+    }
+    return CalculationChooser.openPcaPanel(af, modelName, null);
+  }
+
+  @Override
+  public String getSelectedSequencesAsAlignment(String format,
+          boolean suffix)
+  {
+    return getSelectedSequencesAsAlignmentFrom(null,
+            format, suffix);
+  }
+
+  @Override
+  public String getSelectedSequencesAsAlignmentFrom(AlignFrameI alf,
+          String format, boolean suffix)
+  {
+    if (alf == null)
+    {
+      alf = getCurrentAlignFrame();
+    }
+    return appLoader.getSelectedSequencesAsAlignmentFrom(alf, format,
+            "" + suffix);
+  }
+
+  @Override
+  public String arrayToSeparatorList(String[] array)
+  {
+    return appLoader.arrayToSeparatorList(array);
+  }
+
+  @Override
+  public String[] separatorListToArray(String list)
+  {
+    return appLoader.separatorListToArray(list);
+  }
+
+  //// probably not needed in JalviewJS -- From when Jmol and Jalview did not
+  //// have a direct connection?
+
+  @Override
+  public void setMouseoverListener(String listener)
+  {
+    // TODO Auto-generated method stub
+
+  }
+
+  @Override
+  public void setMouseoverListener(AlignFrameI af, String listener)
+  {
+    // TODO Auto-generated method stub
+
+  }
+
+  @Override
+  public void setSelectionListener(String listener)
+  {
+    // TODO Auto-generated method stub
+
+  }
+
+  @Override
+  public void setSelectionListener(AlignFrameI af, String listener)
+  {
+    // TODO Auto-generated method stub
+
+  }
+
+  @Override
+  public void setStructureListener(String listener, String modelSet)
+  {
+    // TODO Auto-generated method stub
+
+  }
+
+  @Override
+  public void removeJavascriptListener(AlignFrameI af, String listener)
+  {
+    // TODO Auto-generated method stub
+
+  }
+
+  @Override
+  public void mouseOverStructure(String pdbResNum, String chain,
+          String pdbfile)
+  {
+    // TODO Auto-generated method stub
+
+  }
+
+  @Override
+  public void showOverview()
+  {
+    currentAlignFrame.overviewMenuItem_actionPerformed(null);
+  }
+
+  public void notifyWorker(AlignCalcWorkerI worker, String status)
+  {
+    // System.out.println("Jalview worker " + worker.getClass().getSimpleName()
+    // + " " + status);
+  }
+
+  /**
+   * flag to allow selected Runnable and Thread processes to run synchronously
+   * 
+   * JAL-3563
+   * 
+   */
+  private static boolean isSynchronous = false;
+
+  /**
+   * Set Jalview to run selected processes synchronously in test and headless
+   * environments.
+   * 
+   * JAL-3563
+   * 
+   * @param b
+   * @author Bob Hanson
+   */
+  public static void setSynchronous(boolean b)
+  {
+    isSynchronous = b;
+  }
+
+  /**
+   * Allows optional synchronous running of a Runnable that would otherwise use
+   * SwingUtilities.invokeLater.
+   * 
+   * JAL-3563
+   * 
+   * @param t
+   * @author Bob Hanson
+   */
+  public static boolean isSynchronous()
+  {
+    return isSynchronous;
+  }
+
+  /**
+   * Allows optional synchronous running of a Runnable that would otherwise use
+   * SwingUtilities.invokeLater.
+   * 
+   * JAL-3563
+   * 
+   * @param t
+   * @author Bob Hanson
+   */
+  public static void execRunnable(Runnable r)
+  {
+    if (isSynchronous())
+    {
+      r.run();
+    }
+    else
+    {
+      SwingUtilities.invokeLater(r);
+    }
+  }
+
+  /**
+   * Allows optional synchronous running of a thread that would otherwise be run
+   * using start().
+   * 
+   * JAL-3563
+   * 
+   * @param t
+   * @author Bob Hanson
+   */
+  public static void execThread(Thread t)
+  {
+    if (isSynchronous())
+    {
+      t.run();
+    }
+    else
+    {
+      t.start();
+    }
+  }
+
+  /**
+   * Get the SwingJS applet ID and combine that with the frameType
+   * 
+   * @param frameType
+   *          "alignment", "desktop", etc., or null
+   * @return
+   */
+  public static String getAppID(String frameType)
+  {
+    String id = Cache.getProperty("Info.j2sAppletID");
+    if (id == null)
+    {
+      id = "jalview";
+    }
+    return id + (frameType == null ? "" : "-" + frameType);
+  }
+
+}
\ No newline at end of file