JAL-1632 removed check for aligned sequences for Tree calculation
[jalview.git] / src / jalview / gui / AlignFrame.java
index df2a742..bed434d 100644 (file)
@@ -35,6 +35,7 @@ import jalview.api.FeatureSettingsControllerI;
 import jalview.api.SplitContainerI;
 import jalview.api.ViewStyleI;
 import jalview.api.analysis.ScoreModelI;
+import jalview.api.analysis.SimilarityParamsI;
 import jalview.bin.Cache;
 import jalview.bin.Jalview;
 import jalview.commands.CommandI;
@@ -59,6 +60,7 @@ import jalview.datamodel.SeqCigar;
 import jalview.datamodel.Sequence;
 import jalview.datamodel.SequenceGroup;
 import jalview.datamodel.SequenceI;
+import jalview.gui.ColourMenuHelper.ColourChangeListener;
 import jalview.gui.ViewSelectionMenu.ViewSetProvider;
 import jalview.io.AlignmentProperties;
 import jalview.io.AnnotationFile;
@@ -66,7 +68,9 @@ import jalview.io.BioJsHTMLOutput;
 import jalview.io.DataSourceType;
 import jalview.io.FileFormat;
 import jalview.io.FileFormatI;
+import jalview.io.FileFormats;
 import jalview.io.FileLoader;
+import jalview.io.FileParse;
 import jalview.io.FormatAdapter;
 import jalview.io.HtmlSvgOutput;
 import jalview.io.IdentifyFile;
@@ -75,28 +79,16 @@ import jalview.io.JalviewFileChooser;
 import jalview.io.JalviewFileView;
 import jalview.io.JnetAnnotationMaker;
 import jalview.io.NewickFile;
+import jalview.io.ScoreMatrixFile;
 import jalview.io.TCoffeeScoreFile;
 import jalview.jbgui.GAlignFrame;
-import jalview.schemes.Blosum62ColourScheme;
-import jalview.schemes.BuriedColourScheme;
-import jalview.schemes.ClustalxColourScheme;
 import jalview.schemes.ColourSchemeI;
-import jalview.schemes.ColourSchemeProperty;
-import jalview.schemes.HelixColourScheme;
-import jalview.schemes.HydrophobicColourScheme;
-import jalview.schemes.NucleotideColourScheme;
-import jalview.schemes.PIDColourScheme;
-import jalview.schemes.PurinePyrimidineColourScheme;
-import jalview.schemes.RNAHelicesColourChooser;
-import jalview.schemes.ResidueProperties;
-import jalview.schemes.StrandColourScheme;
+import jalview.schemes.ColourSchemes;
+import jalview.schemes.ResidueColourScheme;
 import jalview.schemes.TCoffeeColourScheme;
-import jalview.schemes.TaylorColourScheme;
-import jalview.schemes.TurnColourScheme;
-import jalview.schemes.UserColourScheme;
-import jalview.schemes.ZappoColourScheme;
 import jalview.util.MessageManager;
 import jalview.viewmodel.AlignmentViewport;
+import jalview.viewmodel.ViewportRanges;
 import jalview.ws.DBRefFetcher;
 import jalview.ws.DBRefFetcher.FetchFinishedListenerI;
 import jalview.ws.jws1.Discoverer;
@@ -125,12 +117,13 @@ import java.awt.event.ItemEvent;
 import java.awt.event.ItemListener;
 import java.awt.event.KeyAdapter;
 import java.awt.event.KeyEvent;
-import java.awt.event.MouseAdapter;
 import java.awt.event.MouseEvent;
 import java.awt.print.PageFormat;
 import java.awt.print.PrinterJob;
 import java.beans.PropertyChangeEvent;
 import java.io.File;
+import java.io.FileWriter;
+import java.io.PrintWriter;
 import java.net.URL;
 import java.util.ArrayList;
 import java.util.Arrays;
@@ -146,7 +139,6 @@ import javax.swing.JInternalFrame;
 import javax.swing.JLayeredPane;
 import javax.swing.JMenu;
 import javax.swing.JMenuItem;
-import javax.swing.JRadioButtonMenuItem;
 import javax.swing.JScrollPane;
 import javax.swing.SwingUtilities;
 
@@ -157,7 +149,7 @@ import javax.swing.SwingUtilities;
  * @version $Revision$
  */
 public class AlignFrame extends GAlignFrame implements DropTargetListener,
-        IProgressIndicator, AlignViewControllerGuiI
+        IProgressIndicator, AlignViewControllerGuiI, ColourChangeListener
 {
 
   public static final int DEFAULT_WIDTH = 700;
@@ -171,6 +163,8 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
 
   AlignViewport viewport;
 
+  ViewportRanges vpRanges;
+
   public AlignViewControllerI avc;
 
   List<AlignmentPanel> alignPanels = new ArrayList<AlignmentPanel>();
@@ -342,11 +336,12 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
       progressBar = new ProgressBar(this.statusPanel, this.statusBar);
     }
 
+    vpRanges = viewport.getRanges();
     avc = new jalview.controller.AlignViewController(this, viewport,
             alignPanel);
     if (viewport.getAlignmentConservationAnnotation() == null)
     {
-      BLOSUM62Colour.setEnabled(false);
+      // BLOSUM62Colour.setEnabled(false);
       conservationMenuItem.setEnabled(false);
       modifyConservation.setEnabled(false);
       // PIDColour.setEnabled(false);
@@ -366,19 +361,28 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
       sortPairwiseMenuItem_actionPerformed(null);
     }
 
-    if (Desktop.desktop != null)
-    {
-      this.setDropTarget(new java.awt.dnd.DropTarget(this, this));
-      addServiceListeners();
-      setGUINucleotide(viewport.getAlignment().isNucleotide());
-    }
-
     this.alignPanel.av
             .setShowAutocalculatedAbove(isShowAutoCalculatedAbove());
 
     setMenusFromViewport(viewport);
     buildSortByAnnotationScoresMenu();
-    buildTreeMenu();
+    calculateTree.addActionListener(new ActionListener()
+    {
+
+      @Override
+      public void actionPerformed(ActionEvent e)
+      {
+        openTreePcaDialog();
+      }
+    });
+    buildColourMenu();
+
+    if (Desktop.desktop != null)
+    {
+      this.setDropTarget(new java.awt.dnd.DropTarget(this, this));
+      addServiceListeners();
+      setGUINucleotide();
+    }
 
     if (viewport.getWrapAlignment())
     {
@@ -650,8 +654,10 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
                   new String[] { (viewport.cursorMode ? "on" : "off") }));
           if (viewport.cursorMode)
           {
-            alignPanel.getSeqPanel().seqCanvas.cursorX = viewport.startRes;
-            alignPanel.getSeqPanel().seqCanvas.cursorY = viewport.startSeq;
+            alignPanel.getSeqPanel().seqCanvas.cursorX = vpRanges
+                    .getStartRes();
+            alignPanel.getSeqPanel().seqCanvas.cursorY = vpRanges
+                    .getStartSeq();
           }
           alignPanel.getSeqPanel().seqCanvas.repaint();
           break;
@@ -689,8 +695,8 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
           }
           else
           {
-            alignPanel.setScrollValues(viewport.startRes, viewport.startSeq
-                    - viewport.endSeq + viewport.startSeq);
+            alignPanel.setScrollValues(vpRanges.getStartRes(),
+                    2 * vpRanges.getStartSeq() - vpRanges.getEndSeq());
           }
           break;
         case KeyEvent.VK_PAGE_DOWN:
@@ -700,8 +706,8 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
           }
           else
           {
-            alignPanel.setScrollValues(viewport.startRes, viewport.startSeq
-                    + viewport.endSeq - viewport.startSeq);
+            alignPanel.setScrollValues(vpRanges.getStartRes(),
+                    vpRanges.getEndSeq());
           }
           break;
         }
@@ -845,27 +851,23 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
   /**
    * Configure menu items that vary according to whether the alignment is
    * nucleotide or protein
-   * 
-   * @param nucleotide
    */
-  public void setGUINucleotide(boolean nucleotide)
+  public void setGUINucleotide()
   {
+    AlignmentI al = getViewport().getAlignment();
+    boolean nucleotide = al.isNucleotide();
+
     showTranslation.setVisible(nucleotide);
     showReverse.setVisible(nucleotide);
     showReverseComplement.setVisible(nucleotide);
     conservationMenuItem.setEnabled(!nucleotide);
-    modifyConservation.setEnabled(!nucleotide);
+    modifyConservation.setEnabled(!nucleotide
+            && conservationMenuItem.isSelected());
     showGroupConservation.setEnabled(!nucleotide);
-    rnahelicesColour.setEnabled(nucleotide);
-    nucleotideColour.setEnabled(nucleotide);
-    purinePyrimidineColour.setEnabled(nucleotide);
-    RNAInteractionColour.setEnabled(nucleotide);
+
     showComplementMenuItem.setText(nucleotide ? MessageManager
             .getString("label.protein") : MessageManager
             .getString("label.nucleotide"));
-    setColourSelected(jalview.bin.Cache.getDefault(
-            nucleotide ? Preferences.DEFAULT_COLOUR_NUC
-                    : Preferences.DEFAULT_COLOUR_PROT, "None"));
   }
 
   /**
@@ -891,7 +893,9 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     padGapsMenuitem.setSelected(av.isPadGaps());
     colourTextMenuItem.setSelected(av.isShowColourText());
     abovePIDThreshold.setSelected(av.getAbovePIDThreshold());
+    modifyPID.setEnabled(abovePIDThreshold.isSelected());
     conservationMenuItem.setSelected(av.getConservationSelected());
+    modifyConservation.setEnabled(conservationMenuItem.isSelected());
     seqLimits.setSelected(av.getShowJVSuffix());
     idRightAlign.setSelected(av.isRightAlignIds());
     centreColumnLabelsMenuItem.setState(av.isCentreColumnLabels());
@@ -917,8 +921,8 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     showSequenceLogo.setSelected(av.isShowSequenceLogo());
     normaliseSequenceLogo.setSelected(av.isNormaliseSequenceLogo());
 
-    setColourSelected(ColourSchemeProperty.getColourName(av
-            .getGlobalColourScheme()));
+    ColourMenuHelper.setColourSelected(colourMenu,
+            av.getGlobalColourScheme());
 
     showSeqFeatures.setSelected(av.isShowSequenceFeatures());
     hiddenMarkers.setState(av.getShowHiddenMarkers());
@@ -928,9 +932,6 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     autoCalculate.setSelected(av.autoCalculateConsensus);
     sortByTree.setSelected(av.sortByTree);
     listenToViewSelections.setSelected(av.followSelection);
-    rnahelicesColour.setEnabled(av.getAlignment().hasRNAStructure());
-    rnahelicesColour
-            .setSelected(av.getGlobalColourScheme() instanceof jalview.schemes.RNAHelicesColour);
 
     showProducts.setEnabled(canShowProducts());
     setGroovyEnabled(Desktop.getGroovyConsole() != null);
@@ -1115,7 +1116,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
   public void saveAs_actionPerformed(ActionEvent e)
   {
     String format = currentFileFormat == null ? null : currentFileFormat
-            .toString();
+            .getName();
     JalviewFileChooser chooser = JalviewFileChooser.forWrite(
             Cache.getProperty("LAST_DIRECTORY"), format);
 
@@ -1149,8 +1150,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
 
       fileName = chooser.getSelectedFile().getPath();
 
-      Cache.setProperty("DEFAULT_FILE_FORMAT",
-              currentFileFormat.toString());
+      Cache.setProperty("DEFAULT_FILE_FORMAT", currentFileFormat.getName());
 
       Cache.setProperty("LAST_DIRECTORY", fileName);
       saveAlignment(fileName, currentFileFormat);
@@ -1180,17 +1180,6 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     }
     else
     {
-      // if (!jalview.io.AppletFormatAdapter.isValidFormat(format, true))
-      // {
-      // warningMessage("Cannot save file " + fileName + " using format "
-      // + format, "Alignment output format not supported");
-      // if (!Jalview.isHeadlessMode())
-      // {
-      // saveAs_actionPerformed(null);
-      // }
-      // return false;
-      // }
-
       AlignmentExportData exportData = getAlignmentForExport(format,
               viewport, null);
       if (exportData.getSettings().isCancelled())
@@ -1215,15 +1204,14 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
       {
         try
         {
-          java.io.PrintWriter out = new java.io.PrintWriter(
-                  new java.io.FileWriter(file));
+          PrintWriter out = new PrintWriter(new FileWriter(file));
 
           out.print(output);
           out.close();
           this.setTitle(file);
           statusBar.setText(MessageManager.formatMessage(
                   "label.successfully_saved_to_file_in_format",
-                  new Object[] { fileName, format }));
+                  new Object[] { fileName, format.getName() }));
         } catch (Exception ex)
         {
           success = false;
@@ -1268,8 +1256,8 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
   @Override
   protected void outputText_actionPerformed(ActionEvent e)
   {
-
-    FileFormatI fileFormat = FileFormat.forName(e.getActionCommand());
+    FileFormatI fileFormat = FileFormats.getInstance().forName(
+            e.getActionCommand());
     AlignmentExportData exportData = getAlignmentForExport(fileFormat,
             viewport, null);
     if (exportData.getSettings().isCancelled())
@@ -1282,8 +1270,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     {
       FileFormatI format = fileFormat;
       cap.setText(new FormatAdapter(alignPanel, exportData.getSettings())
-              .formatSequences(format,
-                      exportData.getAlignment(),
+              .formatSequences(format, exportData.getAlignment(),
                       exportData.getOmitHidden(),
                       exportData.getStartEndPostions(),
                       viewport.getColumnSelection()));
@@ -1874,8 +1861,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     }
 
     String output = new FormatAdapter().formatSequences(FileFormat.Fasta,
-            seqs,
-            omitHidden, null);
+            seqs, omitHidden, null);
 
     StringSelection ss = new StringSelection(output);
 
@@ -2171,7 +2157,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
       {
 
         // propagate alignment changed.
-        viewport.setEndSeq(alignment.getHeight());
+        vpRanges.setEndSeq(alignment.getHeight());
         if (annotationAdded)
         {
           // Duplicate sequence annotation in all views.
@@ -2575,7 +2561,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
       {
         trimRegion = new TrimRegionCommand("Remove Left", true, seqs,
                 column, viewport.getAlignment());
-        viewport.setStartRes(0);
+        vpRanges.setStartRes(0);
       }
       else
       {
@@ -2642,13 +2628,13 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     // This is to maintain viewport position on first residue
     // of first sequence
     SequenceI seq = viewport.getAlignment().getSequenceAt(0);
-    int startRes = seq.findPosition(viewport.startRes);
+    int startRes = seq.findPosition(vpRanges.getStartRes());
     // ShiftList shifts;
     // viewport.getAlignment().removeGaps(shifts=new ShiftList());
     // edit.alColumnChanges=shifts.getInverse();
     // if (viewport.hasHiddenColumns)
     // viewport.getColumnSelection().compensateForEdits(shifts);
-    viewport.setStartRes(seq.findIndex(startRes) - 1);
+    vpRanges.setStartRes(seq.findIndex(startRes) - 1);
     viewport.firePropertyChange("alignment", null, viewport.getAlignment()
             .getSequences());
 
@@ -2681,12 +2667,12 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     // This is to maintain viewport position on first residue
     // of first sequence
     SequenceI seq = viewport.getAlignment().getSequenceAt(0);
-    int startRes = seq.findPosition(viewport.startRes);
+    int startRes = seq.findPosition(vpRanges.getStartRes());
 
     addHistoryItem(new RemoveGapsCommand("Remove Gaps", seqs, start, end,
             viewport.getAlignment()));
 
-    viewport.setStartRes(seq.findIndex(startRes) - 1);
+    vpRanges.setStartRes(seq.findIndex(startRes) - 1);
 
     viewport.firePropertyChange("alignment", null, viewport.getAlignment()
             .getSequences());
@@ -2774,6 +2760,14 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
      */
     newap.av.replaceMappings(viewport.getAlignment());
 
+    /*
+     * start up cDNA consensus (if applicable) now mappings are in place
+     */
+    if (newap.av.initComplementConsensus())
+    {
+      newap.refresh(true); // adjust layout of annotations
+    }
+
     newap.av.viewName = getNewViewName(viewTitle);
 
     addAlignmentPanel(newap, true);
@@ -3300,146 +3294,19 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
   }
 
   @Override
-  public void textColour_actionPerformed(ActionEvent e)
+  public void textColour_actionPerformed()
   {
     new TextColourChooser().chooseColour(alignPanel, null);
   }
 
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  protected void noColourmenuItem_actionPerformed(ActionEvent e)
-  {
-    changeColour(null);
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void clustalColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new ClustalxColourScheme(viewport.getAlignment(),
-            viewport.getHiddenRepSequences()));
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void zappoColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new ZappoColourScheme());
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void taylorColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new TaylorColourScheme());
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void hydrophobicityColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new HydrophobicColourScheme());
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void helixColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new HelixColourScheme());
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void strandColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new StrandColourScheme());
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void turnColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new TurnColourScheme());
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void buriedColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new BuriedColourScheme());
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void nucleotideColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new NucleotideColourScheme());
-  }
-
-  @Override
-  public void purinePyrimidineColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new PurinePyrimidineColourScheme());
-  }
-
   /*
-   * public void covariationColour_actionPerformed(ActionEvent e) {
+   * public void covariationColour_actionPerformed() {
    * changeColour(new
    * CovariationColourScheme(viewport.getAlignment().getAlignmentAnnotation
    * ()[0])); }
    */
   @Override
-  public void annotationColour_actionPerformed(ActionEvent e)
+  public void annotationColour_actionPerformed()
   {
     new AnnotationColourChooser(viewport, alignPanel);
   }
@@ -3450,56 +3317,56 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     new AnnotationColumnChooser(viewport, alignPanel);
   }
 
+  /**
+   * Action on the user checking or unchecking the option to apply the selected
+   * colour scheme to all groups. If unchecked, groups may have their own
+   * independent colour schemes.
+   * 
+   * @param selected
+   */
   @Override
-  public void rnahelicesColour_actionPerformed(ActionEvent e)
+  public void applyToAllGroups_actionPerformed(boolean selected)
   {
-    new RNAHelicesColourChooser(viewport, alignPanel);
+    viewport.setColourAppliesToAllGroups(selected);
   }
 
   /**
-   * DOCUMENT ME!
+   * Action on user selecting a colour from the colour menu
    * 
-   * @param e
-   *          DOCUMENT ME!
+   * @param name
+   *          the name (not the menu item label!) of the colour scheme
    */
   @Override
-  protected void applyToAllGroups_actionPerformed(ActionEvent e)
+  public void changeColour_actionPerformed(String name)
   {
-    viewport.setColourAppliesToAllGroups(applyToAllGroups.isSelected());
+    /*
+     * 'User Defined' opens a panel to configure or load a
+     * user-defined colour scheme
+     */
+    if (ResidueColourScheme.USER_DEFINED.equals(name))
+    {
+      new UserDefinedColours(alignPanel);
+      return;
+    }
+
+    /*
+     * otherwise set the chosen colour scheme (or null for 'None')
+     */
+    ColourSchemeI cs = ColourSchemes.getInstance().getColourScheme(name,
+            viewport.getAlignment(), viewport.getHiddenRepSequences());
+    changeColour(cs);
   }
 
   /**
-   * DOCUMENT ME!
+   * Actions on setting or changing the alignment colour scheme
    * 
    * @param cs
-   *          DOCUMENT ME!
    */
   @Override
   public void changeColour(ColourSchemeI cs)
   {
     // TODO: pull up to controller method
-
-    if (cs != null)
-    {
-      // Make sure viewport is up to date w.r.t. any sliders
-      if (viewport.getAbovePIDThreshold())
-      {
-        int threshold = SliderPanel.setPIDSliderSource(alignPanel, cs,
-                "Background");
-        viewport.setThreshold(threshold);
-      }
-
-      if (viewport.getConservationSelected())
-      {
-        cs.setConservationInc(SliderPanel.setConservationSlider(alignPanel,
-                cs, "Background"));
-      }
-      if (cs instanceof TCoffeeColourScheme)
-      {
-        tcoffeeColour.setEnabled(true);
-        tcoffeeColour.setSelected(true);
-      }
-    }
+    ColourMenuHelper.setColourSelected(colourMenu, cs);
 
     viewport.setGlobalColourScheme(cs);
 
@@ -3507,190 +3374,70 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
   }
 
   /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
+   * Show the PID threshold slider panel
    */
   @Override
-  protected void modifyPID_actionPerformed(ActionEvent e)
+  protected void modifyPID_actionPerformed()
   {
-    if (viewport.getAbovePIDThreshold()
-            && viewport.getGlobalColourScheme() != null)
-    {
-      SliderPanel.setPIDSliderSource(alignPanel,
-              viewport.getGlobalColourScheme(), "Background");
-      SliderPanel.showPIDSlider();
-    }
+    SliderPanel.setPIDSliderSource(alignPanel,
+            viewport.getResidueShading(), alignPanel.getViewName());
+    SliderPanel.showPIDSlider();
   }
 
   /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
+   * Show the Conservation slider panel
    */
   @Override
-  protected void modifyConservation_actionPerformed(ActionEvent e)
+  protected void modifyConservation_actionPerformed()
   {
-    if (viewport.getConservationSelected()
-            && viewport.getGlobalColourScheme() != null)
-    {
-      SliderPanel.setConservationSlider(alignPanel,
-              viewport.getGlobalColourScheme(), "Background");
-      SliderPanel.showConservationSlider();
-    }
+    SliderPanel.setConservationSlider(alignPanel,
+            viewport.getResidueShading(), alignPanel.getViewName());
+    SliderPanel.showConservationSlider();
   }
 
   /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
+   * Action on selecting or deselecting (Colour) By Conservation
    */
   @Override
-  protected void conservationMenuItem_actionPerformed(ActionEvent e)
+  public void conservationMenuItem_actionPerformed(boolean selected)
   {
-    viewport.setConservationSelected(conservationMenuItem.isSelected());
-
-    viewport.setAbovePIDThreshold(false);
-    abovePIDThreshold.setSelected(false);
+    modifyConservation.setEnabled(selected);
+    viewport.setConservationSelected(selected);
+    viewport.getResidueShading().setConservationApplied(selected);
 
     changeColour(viewport.getGlobalColourScheme());
-
-    modifyConservation_actionPerformed(null);
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void abovePIDThreshold_actionPerformed(ActionEvent e)
-  {
-    viewport.setAbovePIDThreshold(abovePIDThreshold.isSelected());
-
-    conservationMenuItem.setSelected(false);
-    viewport.setConservationSelected(false);
-
-    changeColour(viewport.getGlobalColourScheme());
-
-    modifyPID_actionPerformed(null);
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void userDefinedColour_actionPerformed(ActionEvent e)
-  {
-    if (e.getActionCommand().equals(
-            MessageManager.getString("action.user_defined")))
+    if (selected)
     {
-      new UserDefinedColours(alignPanel, null);
+      modifyConservation_actionPerformed();
     }
     else
     {
-      UserColourScheme udc = (UserColourScheme) UserDefinedColours
-              .getUserColourSchemes().get(e.getActionCommand());
-
-      changeColour(udc);
+      SliderPanel.hideConservationSlider();
     }
   }
 
-  public void updateUserColourMenu()
+  /**
+   * Action on selecting or deselecting (Colour) Above PID Threshold
+   */
+  @Override
+  public void abovePIDThreshold_actionPerformed(boolean selected)
   {
+    modifyPID.setEnabled(selected);
+    viewport.setAbovePIDThreshold(selected);
+    if (!selected)
+    {
+      viewport.getResidueShading().setThreshold(0,
+              viewport.isIgnoreGapsConsensus());
+    }
 
-    Component[] menuItems = colourMenu.getMenuComponents();
-    int iSize = menuItems.length;
-    for (int i = 0; i < iSize; i++)
+    changeColour(viewport.getGlobalColourScheme());
+    if (selected)
     {
-      if (menuItems[i].getName() != null
-              && menuItems[i].getName().equals("USER_DEFINED"))
-      {
-        colourMenu.remove(menuItems[i]);
-        iSize--;
-      }
+      modifyPID_actionPerformed();
     }
-    if (jalview.gui.UserDefinedColours.getUserColourSchemes() != null)
+    else
     {
-      java.util.Enumeration userColours = jalview.gui.UserDefinedColours
-              .getUserColourSchemes().keys();
-
-      while (userColours.hasMoreElements())
-      {
-        final JRadioButtonMenuItem radioItem = new JRadioButtonMenuItem(
-                userColours.nextElement().toString());
-        radioItem.setName("USER_DEFINED");
-        radioItem.addMouseListener(new MouseAdapter()
-        {
-          @Override
-          public void mousePressed(MouseEvent evt)
-          {
-            if (evt.isPopupTrigger()) // Mac
-            {
-              offerRemoval(radioItem);
-            }
-          }
-
-          @Override
-          public void mouseReleased(MouseEvent evt)
-          {
-            if (evt.isPopupTrigger()) // Windows
-            {
-              offerRemoval(radioItem);
-            }
-          }
-
-          /**
-           * @param radioItem
-           */
-          void offerRemoval(final JRadioButtonMenuItem radioItem)
-          {
-            radioItem.removeActionListener(radioItem.getActionListeners()[0]);
-
-            int option = JvOptionPane.showInternalConfirmDialog(
-                    jalview.gui.Desktop.desktop, MessageManager
-                            .getString("label.remove_from_default_list"),
-                    MessageManager
-                            .getString("label.remove_user_defined_colour"),
-                    JvOptionPane.YES_NO_OPTION);
-            if (option == JvOptionPane.YES_OPTION)
-            {
-              jalview.gui.UserDefinedColours
-                      .removeColourFromDefaults(radioItem.getText());
-              colourMenu.remove(radioItem);
-            }
-            else
-            {
-              radioItem.addActionListener(new ActionListener()
-              {
-                @Override
-                public void actionPerformed(ActionEvent evt)
-                {
-                  userDefinedColour_actionPerformed(evt);
-                }
-              });
-            }
-          }
-        });
-        radioItem.addActionListener(new ActionListener()
-        {
-          @Override
-          public void actionPerformed(ActionEvent evt)
-          {
-            userDefinedColour_actionPerformed(evt);
-          }
-        });
-
-        colourMenu.insert(radioItem, 15);
-        colours.add(radioItem);
-      }
+      SliderPanel.hidePIDSlider();
     }
   }
 
@@ -3701,35 +3448,11 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
    *          DOCUMENT ME!
    */
   @Override
-  public void PIDColour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new PIDColourScheme());
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void BLOSUM62Colour_actionPerformed(ActionEvent e)
-  {
-    changeColour(new Blosum62ColourScheme());
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
   public void sortPairwiseMenuItem_actionPerformed(ActionEvent e)
   {
     SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
     AlignmentSorter.sortByPID(viewport.getAlignment(), viewport
-            .getAlignment().getSequenceAt(0), null);
+            .getAlignment().getSequenceAt(0));
     addHistoryItem(new OrderCommand("Pairwise Sort", oldOrder,
             viewport.getAlignment()));
     alignPanel.paintAlignment(true);
@@ -3876,65 +3599,18 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
   }
 
   /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void averageDistanceTreeMenuItem_actionPerformed(ActionEvent e)
-  {
-    newTreePanel("AV", "PID", "Average distance tree using PID");
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  public void neighbourTreeMenuItem_actionPerformed(ActionEvent e)
-  {
-    newTreePanel("NJ", "PID", "Neighbour joining tree using PID");
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  protected void njTreeBlosumMenuItem_actionPerformed(ActionEvent e)
-  {
-    newTreePanel("NJ", "BL", "Neighbour joining tree using BLOSUM62");
-  }
-
-  /**
-   * DOCUMENT ME!
-   * 
-   * @param e
-   *          DOCUMENT ME!
-   */
-  @Override
-  protected void avTreeBlosumMenuItem_actionPerformed(ActionEvent e)
-  {
-    newTreePanel("AV", "BL", "Average distance tree using BLOSUM62");
-  }
-
-  /**
-   * DOCUMENT ME!
+   * Constructs a tree panel and adds it to the desktop
    * 
    * @param type
-   *          DOCUMENT ME!
-   * @param pwType
-   *          DOCUMENT ME!
-   * @param title
-   *          DOCUMENT ME!
+   *          tree type (NJ or AV)
+   * @param sm
+   *          distance or similarity score model used to compute the tree
+   * @param options
+   *          parameters for the distance or similarity calculation
    */
-  void newTreePanel(String type, String pwType, String title)
+  void newTreePanel(String type, ScoreModelI sm, SimilarityParamsI options)
   {
+    String frameTitle = "";
     TreePanel tp;
 
     if (viewport.getSelectionGroup() != null
@@ -3973,44 +3649,30 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
         }
       }
 
-      title = title + " on region";
-      tp = new TreePanel(alignPanel, type, pwType);
+      tp = new TreePanel(alignPanel, type, sm, options);
+      frameTitle = tp.getPanelTitle() + " on region";
     }
     else
     {
-      // are the visible sequences aligned?
-      if (!viewport.getAlignment().isAligned(false))
-      {
-        JvOptionPane
-                .showMessageDialog(
-                        Desktop.desktop,
-                        MessageManager
-                                .getString("label.sequences_must_be_aligned_before_creating_tree"),
-                        MessageManager
-                                .getString("label.sequences_not_aligned"),
-                        JvOptionPane.WARNING_MESSAGE);
-
-        return;
-      }
-
       if (viewport.getAlignment().getHeight() < 2)
       {
         return;
       }
 
-      tp = new TreePanel(alignPanel, type, pwType);
+      tp = new TreePanel(alignPanel, type, sm, options);
+      frameTitle = tp.getPanelTitle();
     }
 
-    title += " from ";
+    frameTitle += " from ";
 
     if (viewport.viewName != null)
     {
-      title += viewport.viewName + " of ";
+      frameTitle += viewport.viewName + " of ";
     }
 
-    title += this.title;
+    frameTitle += this.title;
 
-    Desktop.addInternalFrame(tp, title, 600, 500);
+    Desktop.addInternalFrame(tp, frameTitle, 600, 500);
   }
 
   /**
@@ -4132,48 +3794,10 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
    * call. Listeners are added to remove the menu item when the treePanel is
    * closed, and adjust the tree leaf to sequence mapping when the alignment is
    * modified.
-   * 
-   * @param treePanel
-   *          Displayed tree window.
-   * @param title
-   *          SortBy menu item title.
    */
   @Override
-  public void buildTreeMenu()
+  public void buildTreeSortMenu()
   {
-    calculateTree.removeAll();
-    // build the calculate menu
-
-    for (final String type : new String[] { "NJ", "AV" })
-    {
-      String treecalcnm = MessageManager.getString("label.tree_calc_"
-              + type.toLowerCase());
-      for (final String pwtype : ResidueProperties.scoreMatrices.keySet())
-      {
-        JMenuItem tm = new JMenuItem();
-        ScoreModelI sm = ResidueProperties.scoreMatrices.get(pwtype);
-        if (sm.isDNA() == viewport.getAlignment().isNucleotide()
-                || sm.isProtein() == !viewport.getAlignment()
-                        .isNucleotide())
-        {
-          String smn = MessageManager.getStringOrReturn(
-                  "label.score_model_", sm.getName());
-          final String title = MessageManager.formatMessage(
-                  "label.treecalc_title", treecalcnm, smn);
-          tm.setText(title);//
-          tm.addActionListener(new java.awt.event.ActionListener()
-          {
-            @Override
-            public void actionPerformed(ActionEvent e)
-            {
-              newTreePanel(type, pwtype, title);
-            }
-          });
-          calculateTree.add(tm);
-        }
-
-      }
-    }
     sortByTreeMenu.removeAll();
 
     List<Component> comps = PaintRefresher.components.get(viewport
@@ -4324,13 +3948,13 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
 
     if (value == JalviewFileChooser.APPROVE_OPTION)
     {
-      String choice = chooser.getSelectedFile().getPath();
-      jalview.bin.Cache.setProperty("LAST_DIRECTORY", choice);
-      jalview.io.NewickFile fin = null;
+      String filePath = chooser.getSelectedFile().getPath();
+      Cache.setProperty("LAST_DIRECTORY", filePath);
+      NewickFile fin = null;
       try
       {
-        fin = new NewickFile(choice, DataSourceType.FILE);
-        viewport.setCurrentTree(ShowNewickTree(fin, choice).getTree());
+        fin = new NewickFile(filePath, DataSourceType.FILE);
+        viewport.setCurrentTree(showNewickTree(fin, filePath).getTree());
       } catch (Exception ex)
       {
         JvOptionPane
@@ -4352,31 +3976,19 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     }
   }
 
-  @Override
-  protected void tcoffeeColorScheme_actionPerformed(ActionEvent e)
-  {
-    changeColour(new TCoffeeColourScheme(alignPanel.getAlignment()));
-  }
-
-  public TreePanel ShowNewickTree(NewickFile nf, String title)
-  {
-    return ShowNewickTree(nf, title, 600, 500, 4, 5);
-  }
-
-  public TreePanel ShowNewickTree(NewickFile nf, String title,
-          AlignmentView input)
+  public TreePanel showNewickTree(NewickFile nf, String treeTitle)
   {
-    return ShowNewickTree(nf, title, input, 600, 500, 4, 5);
+    return showNewickTree(nf, treeTitle, 600, 500, 4, 5);
   }
 
-  public TreePanel ShowNewickTree(NewickFile nf, String title, int w,
+  public TreePanel showNewickTree(NewickFile nf, String treeTitle, int w,
           int h, int x, int y)
   {
-    return ShowNewickTree(nf, title, null, w, h, x, y);
+    return showNewickTree(nf, treeTitle, null, w, h, x, y);
   }
 
   /**
-   * Add a treeviewer for the tree extracted from a newick file object to the
+   * Add a treeviewer for the tree extracted from a Newick file object to the
    * current alignment view
    * 
    * @param nf
@@ -4395,7 +4007,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
    *          position
    * @return TreePanel handle
    */
-  public TreePanel ShowNewickTree(NewickFile nf, String title,
+  public TreePanel showNewickTree(NewickFile nf, String treeTitle,
           AlignmentView input, int w, int h, int x, int y)
   {
     TreePanel tp = null;
@@ -4406,7 +4018,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
 
       if (nf.getTree() != null)
       {
-        tp = new TreePanel(alignPanel, "FromFile", title, nf, input);
+        tp = new TreePanel(alignPanel, nf, treeTitle, input);
 
         tp.setSize(w, h);
 
@@ -4415,7 +4027,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
           tp.setLocation(x, y);
         }
 
-        Desktop.addInternalFrame(tp, title, w, h);
+        Desktop.addInternalFrame(tp, treeTitle, w, h);
       }
     } catch (Exception ex)
     {
@@ -4988,10 +4600,13 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
   }
 
   /**
-   * Attempt to load a "dropped" file or URL string: First by testing whether
-   * it's an Annotation file, then a JNet file, and finally a features file. If
-   * all are false then the user may have dropped an alignment file onto this
-   * AlignFrame.
+   * Attempt to load a "dropped" file or URL string, by testing in turn for
+   * <ul>
+   * <li>an Annotation file</li>
+   * <li>a JNet file</li>
+   * <li>a features file</li>
+   * <li>else try to interpret as an alignment file</li>
+   * </ul>
    * 
    * @param file
    *          either a filename or a URL string.
@@ -5023,8 +4638,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
           {
             if (tcf.annotateAlignment(viewport.getAlignment(), true))
             {
-              tcoffeeColour.setEnabled(true);
-              tcoffeeColour.setSelected(true);
+              buildColourMenu();
               changeColour(new TCoffeeColourScheme(viewport.getAlignment()));
               isAnnotation = true;
               statusBar
@@ -5066,10 +4680,20 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
           {
             format = new IdentifyFile().identify(file, sourceType);
           }
-          if (FileFormat.Jnet.equals(format))
+          if (FileFormat.ScoreMatrix == format)
+          {
+            ScoreMatrixFile sm = new ScoreMatrixFile(new FileParse(file,
+                    sourceType));
+            sm.parse();
+            // todo: i18n this message
+            statusBar
+                    .setText(MessageManager.formatMessage(
+                            "label.successfully_loaded_matrix",
+                            sm.getMatrixName()));
+          }
+          else if (FileFormat.Jnet.equals(format))
           {
-            JPredFile predictions = new JPredFile(
-                    file, sourceType);
+            JPredFile predictions = new JPredFile(file, sourceType);
             new JnetAnnotationMaker();
             JnetAnnotationMaker.add_annotation(predictions,
                     viewport.getAlignment(), 0, false);
@@ -5139,6 +4763,28 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     }
 
     /*
+     * 'focus' any colour slider that is open to the selected viewport
+     */
+    if (viewport.getConservationSelected())
+    {
+      SliderPanel.setConservationSlider(alignPanel,
+              viewport.getResidueShading(), alignPanel.getViewName());
+    }
+    else
+    {
+      SliderPanel.hideConservationSlider();
+    }
+    if (viewport.getAbovePIDThreshold())
+    {
+      SliderPanel.setPIDSliderSource(alignPanel,
+              viewport.getResidueShading(), alignPanel.getViewName());
+    }
+    else
+    {
+      SliderPanel.hidePIDSlider();
+    }
+
+    /*
      * If there is a frame linked to this one in a SplitPane, switch it to the
      * same view tab index. No infinite recursion of calls should happen, since
      * tabSelectionChanged() should not get invoked on setting the selected
@@ -5838,7 +5484,7 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
     }
     AlignmentI cdna = new Alignment(cdnaSeqs.toArray(new SequenceI[cdnaSeqs
             .size()]));
-    AlignFrame alignFrame = new AlignFrame(cdna, AlignFrame.DEFAULT_WIDTH,
+    GAlignFrame alignFrame = new AlignFrame(cdna, AlignFrame.DEFAULT_WIDTH,
             AlignFrame.DEFAULT_HEIGHT);
     cdna.alignAs(alignment);
     String newtitle = "cDNA " + MessageManager.getString("label.for") + " "
@@ -5954,6 +5600,44 @@ public class AlignFrame extends GAlignFrame implements DropTargetListener,
             true,
             (actionEvent.getModifiers() & (ActionEvent.META_MASK | ActionEvent.CTRL_MASK)) != 0);
   }
+
+  /**
+   * Rebuilds the Colour menu, including any user-defined colours which have
+   * been loaded either on startup or during the session
+   */
+  public void buildColourMenu()
+  {
+    colourMenu.removeAll();
+
+    colourMenu.add(applyToAllGroups);
+    colourMenu.add(textColour);
+    colourMenu.addSeparator();
+
+    ColourMenuHelper.addMenuItems(colourMenu, this,
+            viewport.getAlignment(), false);
+
+    colourMenu.addSeparator();
+    colourMenu.add(conservationMenuItem);
+    colourMenu.add(modifyConservation);
+    colourMenu.add(abovePIDThreshold);
+    colourMenu.add(modifyPID);
+    colourMenu.add(annotationColour);
+
+    ColourSchemeI colourScheme = viewport.getGlobalColourScheme();
+    ColourMenuHelper.setColourSelected(colourMenu, colourScheme);
+  }
+
+  /**
+   * Open a dialog (if not already open) that allows the user to select and
+   * calculate PCA or Tree analysis
+   */
+  protected void openTreePcaDialog()
+  {
+    if (alignPanel.getCalculationDialog() == null)
+    {
+      new CalculationChooser(AlignFrame.this);
+    }
+  }
 }
 
 class PrintThread extends Thread