package jalview.io;
import jalview.datamodel.CigarParser;
+import jalview.datamodel.Range;
import jalview.datamodel.Sequence;
import jalview.datamodel.SequenceFeature;
import jalview.datamodel.SequenceI;
import java.io.File;
import java.io.IOException;
+import java.net.URL;
import java.util.ArrayList;
import java.util.List;
+import java.util.Map;
import java.util.PrimitiveIterator.OfInt;
import java.util.SortedMap;
import htsjdk.samtools.SAMRecord;
import htsjdk.samtools.SAMRecordIterator;
import htsjdk.samtools.SAMSequenceRecord;
+import htsjdk.samtools.SamInputResource;
import htsjdk.samtools.SamReader;
import htsjdk.samtools.SamReaderFactory;
import htsjdk.samtools.ValidationStringency;
public BamFile(FileParse source) throws IOException
{
super(true, source);
+ parseSuffix();
final SamReaderFactory factory = SamReaderFactory.makeDefault()
.enable(SamReaderFactory.Option.INCLUDE_SOURCE_IN_RECORDS,
SamReaderFactory.Option.VALIDATE_CRC_CHECKSUMS)
.validationStringency(ValidationStringency.SILENT);
// File-based bam
- fileReader = factory.open(source.inFile);
+ if (source.getDataSourceType() == DataSourceType.FILE)
+ {
+ fileReader = factory.open(source.inFile);
+ }
+ else
+ {
+ // locate index ?
+ String index = source.getDataName() + ".bai";
+ fileReader = factory.open(SamInputResource.of(source.getDataName())
+ .index(new URL(index)));
+ }
}
@Override
return null;
}
+ private StringBuilder insertRefSeq(
+ SortedMap<Integer, Integer> insertions, Range xtent)
+ {
+ StringBuilder refseq = new StringBuilder();
+ int inserted = 0;
+ for (int p = xtent.start; p < xtent.end; p++)
+ {
+ refseq.append('N');
+ }
+ for (Map.Entry<Integer, Integer> insert : insertions.entrySet())
+ {
+ int inspos = insert.getKey() - xtent.start + inserted;
+ if (inspos < 0)
+ {
+ System.out.println("Ignoring -ve insert position " + insert.getKey()
+ + " of " + insert.getValue() +
+ " (alpos: " + inspos + ")");
+ inspos = 0;
+ // continue;
+ }
+ for (int i = 0, j = insert.getValue(); i < j; i++)
+ {
+ inserted++;
+ refseq.insert(inspos, '-');
+ }
+ }
+ return refseq;
+ }
+
+ private void padRef(SequenceI ref, CigarParser cig)
+ { // pad to column
+ int padding = cig.firstAlColumn - ref.findIndex(cig.firstRposCol);
+ System.out.println("Padding " + padding + " to move refseq position "
+ + cig.firstRposCol + " to " + cig.firstAlColumn + "col.");
+
+ ref.insertCharAt(0, padding, '-');
+ }
+
@Override
public void parse()
{
SAMRecordIterator it = fileReader.query(chromosome, start, end,
false);
CigarParser parser = new CigarParser('-');
- SortedMap<Integer, Integer> insertions = parser.getInsertions(it);
+ Range[] xtent = new Range[] { new Range(start, end) };
+ SortedMap<Integer, Integer> insertions[] = parser.getInsertions(it,
+ xtent);
it.close();
+ SequenceI refSeq = new Sequence("chr:" + chromosome,
+ insertRefSeq(insertions[0], xtent[0])
+ .toString());
+
+ refSeq.setStart(xtent[0].start);
+ refSeq.setEnd(xtent[0].end);
+ SequenceI revRefSeq = new Sequence("rev:chr:" + chromosome,
+ insertRefSeq(insertions[1], xtent[0])
+ .toString());
+ revRefSeq.setStart(xtent[0].start);
+ revRefSeq.setEnd(xtent[0].end);
+
+ // Hack to move the seqs along
+ padRef(refSeq, parser);
+ padRef(revRefSeq, parser);
+
it = fileReader.query(chromosome, start, end, false);
+
+ ArrayList<SequenceI> fwd = new ArrayList(), rev = new ArrayList();
while (it.hasNext())
{
SAMRecord rec = it.next();
}
// make dataset sequence: start at 1, end at read length
- SequenceI seq = new Sequence(rec.getReadName(),
+ SequenceI seq = new Sequence(
+ "" + (rec.getReadNegativeStrandFlag() ? "rev:" : "")
+ + rec.getReadName(),
rec.getReadString().toLowerCase());
seq.setStart(1);
seq.setEnd(rec.getReadLength());
(float) q.next() - ' ', "bamfile"));
p++;
}
- String newRead = parser.parseCigarToSequence(rec, insertions,
+ String newRead = parser.parseCigarToSequence(rec,
+ insertions[rec.getReadNegativeStrandFlag() ? 1 : 0],
alignmentStart, seq);
// make alignment sequences
// set start relative to soft clip; assume end is set by Sequence code
alsq.setStart(rec.getStart() - rec.getUnclippedStart() + 1);
- seqs.add(alsq);
+ if (rec.getReadNegativeStrandFlag())
+ {
+ rev.add(alsq);
+ }
+ else
+ {
+ fwd.add(alsq);
+ }
+ }
+ // Add forward
+ if (fwd.size() > 0)
+ {
+ seqs.add(refSeq); // FIXME needs to be optional, and properly padded
+ seqs.addAll(fwd);
+ fwd.clear();
+ }
+ // and reverse strand reads.
+ if (rev.size() > 0)
+ {
+ seqs.add(revRefSeq); // FIXME needs to be optional and properly padded
+ seqs.addAll(rev);
+ rev.clear();
}
}
}
chromosome = chr;
start = s;
end = e;
+ suffix = chromosome + ":" + start + "-" + end;
+ }
+
+ public boolean parseSuffix()
+ {
+ if (suffix == null)
+ {
+ return false;
+ }
+ int csep = suffix.indexOf(":");
+ int rsep = suffix.indexOf("-", csep);
+ if (csep < 0 || rsep < 0 || suffix.length() - rsep <= 1)
+ {
+ return false;
+ }
+ String chr, p1, p2;
+ chr = suffix.substring(0, csep);
+ p1 = suffix.substring(csep + 1, rsep);
+ p2 = suffix.substring(rsep + 1);
+ int cstart, cend;
+ try
+ {
+ cstart = Integer.parseInt(p1);
+ cend = Integer.parseInt(p2);
+ } catch (Exception e)
+ {
+ warningMessage = (warningMessage == null ? "" : warningMessage + "\n")
+ + "Couldn't parse range from " + suffix;
+ return false;
+ }
+ chromosome = chr;
+ start = cstart;
+ end = cend;
+ return true;
}
}
git://source.jalview.org / jalview.git / blobdiff