--- /dev/null
+package jalview.analysis;
+
+import static org.testng.Assert.assertEquals;
+import static org.testng.Assert.assertFalse;
+import static org.testng.Assert.assertNull;
+import static org.testng.Assert.assertSame;
+
+import java.util.Iterator;
+
+import org.testng.annotations.Test;
+
+public class GeneticCodesTest
+{
+ @Test(groups = "Functional")
+ public void testGetCodeTable()
+ {
+ GeneticCodes codes = GeneticCodes.getInstance();
+ assertEquals(codes.getStandardCodeTable().getName(), "Standard");
+ assertEquals(codes.getStandardCodeTable().getId(), "1");
+ assertSame(codes.getStandardCodeTable(), codes.getCodeTable("1"));
+ assertEquals(codes.getCodeTable("2").getName(),
+ "Vertebrate Mitochondrial");
+ assertEquals(codes.getCodeTable("11").getName(),
+ "Bacterial, Archaeal and Plant Plastid");
+ assertEquals(codes.getCodeTable("31").getName(),
+ "Blastocrithidia Nuclear");
+ }
+
+ @Test(groups = "Functional")
+ public void testGetCodeTables()
+ {
+ GeneticCodes codes = GeneticCodes.getInstance();
+ Iterator<GeneticCodeI> tableIterator = codes.getCodeTables().iterator();
+ String[] ids = new String[] { "1", "2", "3", "4", "5", "6", "9", "10",
+ "11", "12", "13", "14", "16", "21", "22", "23", "24", "25", "26",
+ "27", "28", "29", "30", "31" };
+ for (int i = 0; i < ids.length; i++)
+ {
+ assertEquals(tableIterator.next().getId(), ids[i]);
+ }
+ assertFalse(tableIterator.hasNext());
+ }
+
+ @Test(groups = "Functional")
+ public void testTranslate()
+ {
+ GeneticCodes codes = GeneticCodes.getInstance();
+
+ GeneticCodeI gc = codes.getCodeTable("1");
+ assertNull(gc.translate("XYZ"));
+ assertEquals(gc.translate("AGA"), "R");
+
+ gc = codes.getCodeTable("2");
+ assertEquals(gc.translate("AGA"), "*"); // variant
+ assertEquals(gc.translate("ttc"), "F"); // non-variant
+
+ // table 11 has no variant translations - should serve the standard values
+ gc = codes.getCodeTable("11");
+ assertEquals(gc.translate("ttc"), "F");
+
+ gc = codes.getCodeTable("31");
+ assertEquals(gc.translate("TGA"), "W"); // variant
+ assertEquals(gc.translate("tag"), "E"); // variant
+ assertEquals(gc.translate("AGC"), "S"); // non-variant
+ }
+
+ /**
+ * Test 'standard' codon translations (no ambiguity codes)
+ */
+ @Test(groups = { "Functional" })
+ public void testTranslate_standardTable()
+ {
+ GeneticCodeI st = GeneticCodes.getInstance().getStandardCodeTable();
+ assertEquals("F", st.translate("TTT"));
+ assertEquals("F", st.translate("TTC"));
+ assertEquals("L", st.translate("TTA"));
+ assertEquals("L", st.translate("TTG"));
+ assertEquals("L", st.translate("CTT"));
+ assertEquals("L", st.translate("CTC"));
+ assertEquals("L", st.translate("CTA"));
+ assertEquals("L", st.translate("CTG"));
+ assertEquals("I", st.translate("ATT"));
+ assertEquals("I", st.translate("ATC"));
+ assertEquals("I", st.translate("ATA"));
+ assertEquals("M", st.translate("ATG"));
+ assertEquals("V", st.translate("GTT"));
+ assertEquals("V", st.translate("GTC"));
+ assertEquals("V", st.translate("GTA"));
+ assertEquals("V", st.translate("GTG"));
+ assertEquals("S", st.translate("TCT"));
+ assertEquals("S", st.translate("TCC"));
+ assertEquals("S", st.translate("TCA"));
+ assertEquals("S", st.translate("TCG"));
+ assertEquals("P", st.translate("CCT"));
+ assertEquals("P", st.translate("CCC"));
+ assertEquals("P", st.translate("CCA"));
+ assertEquals("P", st.translate("CCG"));
+ assertEquals("T", st.translate("ACT"));
+ assertEquals("T", st.translate("ACC"));
+ assertEquals("T", st.translate("ACA"));
+ assertEquals("T", st.translate("ACG"));
+ assertEquals("A", st.translate("GCT"));
+ assertEquals("A", st.translate("GCC"));
+ assertEquals("A", st.translate("GCA"));
+ assertEquals("A", st.translate("GCG"));
+ assertEquals("Y", st.translate("TAT"));
+ assertEquals("Y", st.translate("TAC"));
+ assertEquals("*", st.translate("TAA"));
+ assertEquals("*", st.translate("TAG"));
+ assertEquals("H", st.translate("CAT"));
+ assertEquals("H", st.translate("CAC"));
+ assertEquals("Q", st.translate("CAA"));
+ assertEquals("Q", st.translate("CAG"));
+ assertEquals("N", st.translate("AAT"));
+ assertEquals("N", st.translate("AAC"));
+ assertEquals("K", st.translate("AAA"));
+ assertEquals("K", st.translate("AAG"));
+ assertEquals("D", st.translate("GAT"));
+ assertEquals("D", st.translate("GAC"));
+ assertEquals("E", st.translate("GAA"));
+ assertEquals("E", st.translate("GAG"));
+ assertEquals("C", st.translate("TGT"));
+ assertEquals("C", st.translate("TGC"));
+ assertEquals("*", st.translate("TGA"));
+ assertEquals("W", st.translate("TGG"));
+ assertEquals("R", st.translate("CGT"));
+ assertEquals("R", st.translate("CGC"));
+ assertEquals("R", st.translate("CGA"));
+ assertEquals("R", st.translate("CGG"));
+ assertEquals("S", st.translate("AGT"));
+ assertEquals("S", st.translate("AGC"));
+ assertEquals("R", st.translate("AGA"));
+ assertEquals("R", st.translate("AGG"));
+ assertEquals("G", st.translate("GGT"));
+ assertEquals("G", st.translate("GGC"));
+ assertEquals("G", st.translate("GGA"));
+ assertEquals("G", st.translate("GGG"));
+ }
+
+ /**
+ * Test a sample of codon translations involving ambiguity codes. Should
+ * return a protein value where the ambiguity does not affect the translation.
+ */
+ @Test(groups = { "Functional" })
+ public void testTranslate_standardTableAmbiguityCodes()
+ {
+ GeneticCodeI st = GeneticCodes.getInstance().getStandardCodeTable();
+ // Y is C or T
+ assertEquals("C", st.translate("TGY"));
+ // Phenylalanine first base variation
+ assertEquals("L", st.translate("YTA"));
+
+ // W is A or T
+ assertEquals("L", st.translate("CTW"));
+ assertNull(st.translate("TTW"));
+
+ // S is G or C
+ assertEquals("G", st.translate("GGS"));
+ assertNull(st.translate("ATS"));
+
+ // K is T or G
+ assertEquals("S", st.translate("TCK"));
+ assertNull(st.translate("ATK"));
+
+ // M is C or A
+ assertEquals("T", st.translate("ACM"));
+ // Arginine first base variation
+ assertEquals("R", st.translate("MGA"));
+ assertEquals("R", st.translate("MGG"));
+ assertNull(st.translate("TAM"));
+
+ // D is A, G or T
+ assertEquals("P", st.translate("CCD"));
+ assertNull(st.translate("AAD"));
+
+ // V is A, C or G
+ assertEquals("V", st.translate("GTV"));
+ assertNull(st.translate("TTV"));
+
+ // H is A, C or T
+ assertEquals("A", st.translate("GCH"));
+ assertEquals("I", st.translate("ATH"));
+ assertNull(st.translate("AGH"));
+
+ // B is C, G or T
+ assertEquals("P", st.translate("CCB"));
+ assertNull(st.translate("TAB"));
+
+ // R is A or G
+ // additional tests for JAL-1685 (resolved)
+ assertEquals("L", st.translate("CTR"));
+ assertEquals("V", st.translate("GTR"));
+ assertEquals("S", st.translate("TCR"));
+ assertEquals("P", st.translate("CCR"));
+ assertEquals("T", st.translate("ACR"));
+ assertEquals("A", st.translate("GCR"));
+ assertEquals("R", st.translate("CGR"));
+ assertEquals("G", st.translate("GGR"));
+ assertEquals("R", st.translate("AGR"));
+ assertEquals("E", st.translate("GAR"));
+ assertEquals("K", st.translate("AAR"));
+ assertEquals("L", st.translate("TTR"));
+ assertEquals("Q", st.translate("CAR"));
+ assertEquals("*", st.translate("TAR"));
+ assertEquals("*", st.translate("TRA"));
+ // Arginine first and third base ambiguity
+ assertEquals("R", st.translate("MGR"));
+ assertNull(st.translate("ATR"));
+
+ // N is any base; 8 proteins accept any base in 3rd position
+ assertEquals("L", st.translate("CTN"));
+ assertEquals("V", st.translate("GTN"));
+ assertEquals("S", st.translate("TCN"));
+ assertEquals("P", st.translate("CCN"));
+ assertEquals("T", st.translate("ACN"));
+ assertEquals("A", st.translate("GCN"));
+ assertEquals("R", st.translate("CGN"));
+ assertEquals("G", st.translate("GGN"));
+ assertNull(st.translate("ATN"));
+ assertNull(st.translate("ANT"));
+ assertNull(st.translate("NAT"));
+ assertNull(st.translate("ANN"));
+ assertNull(st.translate("NNA"));
+ assertNull(st.translate("NNN"));
+
+ // some random stuff
+ assertNull(st.translate("YWB"));
+ assertNull(st.translate("VHD"));
+ assertNull(st.translate("WSK"));
+ }
+
+ /**
+ * Test a sample of codon translations involving ambiguity codes. Should
+ * return a protein value where the ambiguity does not affect the translation.
+ */
+ @Test(groups = { "Functional" })
+ public void testTranslate_nonStandardTableAmbiguityCodes()
+ {
+ GeneticCodeI standard = GeneticCodes.getInstance()
+ .getStandardCodeTable();
+
+ /*
+ * Vertebrate Mitochondrial (Table 2)
+ */
+ GeneticCodeI gc = GeneticCodes.getInstance().getCodeTable("2");
+ // AGR is AGA or AGG - R in standard code, * in table 2
+ assertEquals(gc.translate("AGR"), "*");
+ assertEquals(standard.translate("AGR"), "R");
+ // TGR is TGA or TGG - ambiguous in standard code, W in table 2
+ assertEquals(gc.translate("TGR"), "W");
+ assertNull(standard.translate("TGR"));
+
+ /*
+ * Yeast Mitochondrial (Table 3)
+ */
+ gc = GeneticCodes.getInstance().getCodeTable("3");
+ // CTN is L in standard code, T in table 3
+ assertEquals(gc.translate("ctn"), "T");
+ assertEquals(standard.translate("CTN"), "L");
+
+ /*
+ * Alternative Yeast Nuclear (Table 12)
+ */
+ gc = GeneticCodes.getInstance().getCodeTable("12");
+ // CTG is S; in the standard code CTN is L
+ assertEquals(gc.translate("CTG"), "S");
+ assertNull(gc.translate("CTK")); // K is G or T -> S or L
+ assertEquals(standard.translate("CTK"), "L");
+ assertEquals(gc.translate("CTH"), "L"); // H is anything other than G
+ assertEquals(standard.translate("CTH"), "L");
+ assertEquals(standard.translate("CTN"), "L");
+
+ /*
+ * Trematode Mitochondrial (Table 21)
+ */
+ gc = GeneticCodes.getInstance().getCodeTable("21");
+ // AAR is K in standard code, ambiguous in table 21 as AAA=N not K
+ assertNull(gc.translate("AAR"));
+ assertEquals(standard.translate("AAR"), "K");
+ }
+
+ @Test(groups = "Functional")
+ public void testTranslateCanonical()
+ {
+ GeneticCodes codes = GeneticCodes.getInstance();
+
+ GeneticCodeI gc = codes.getCodeTable("1");
+ assertNull(gc.translateCanonical("XYZ"));
+ assertEquals(gc.translateCanonical("AGA"), "R");
+ // translateCanonical should not resolve ambiguity codes
+ assertNull(gc.translateCanonical("TGY"));
+
+ gc = codes.getCodeTable("2");
+ assertNull(gc.translateCanonical("AGR"));
+ assertEquals(gc.translateCanonical("AGA"), "*"); // variant
+ assertEquals(gc.translateCanonical("ttc"), "F"); // non-variant
+ }
+}
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