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-<head>
-<title>Reference Sequences</title>
-</head>
-<body>
- <p>
- <strong>Reference Sequence Alignment Views</strong>
- </p>
- <p>Many alignment analysis tasks concern a query, or reference
- sequence. For instance, when searching for sequences from other
- organisms that are similar to a newly sequenced gene, or when
- searching for structurally similar sequences for use in homology
- modelling.</p>
- <p>
- <strong>What happens when a reference sequence is defined ?</strong>
- </p>
- <p>The reference sequence for an alignment is indicated by its ID
- being shown in bold. In addition:</p>
- <ul>
- <li><strong>Reference sequence numbering</strong>. Instead of
- column numbers, the alignment ruler shows the reference sequence
- positions at each column. At each tick mark, either the reference
- sequence symbol and position is given, or the column number when a
- gap is present at that position in the reference sequence.</li>
- <li><strong>Format→Show unconserved</strong> highlights
- mutations with respect to the reference sequence, rather than the
- alignment's consensus sequence.</li>
- </ul>
- <p>
- <strong>Defining the reference sequence</strong>
- </p>
- <p>Each alignment view can have its own reference sequence.</p>
- <ul>
- <li><strong>Manually assigning a reference sequence</strong><br />
- A sequence can be marked as the reference sequence by
- right-clicking on its ID to open the popup menu, and selecting the
- "<strong>(Sequence ID)→Mark as Reference</strong>" entry.</li>
- <li><strong>Defining a reference when importing
- annotation</strong><br />Jalview automatically assigns a reference
- sequence when importing analysis results, such as those returned
- from <a href="../webServices/jnet.html">JPred</a> . A reference
- sequence can also be assigned via the <a
- href="../features/annotationsFormat.html#refsandviews">SET_REF</a>
- command in an alignment annotation file.</li>
- </ul>
- <p>
- <em>Reference sequence based alignment visualisation was
- introduced in Jalview 2.9, and support for storage and retrieval
- of reference sequence views in 2.10.</em>
- </p>
-</html>