*/
public class AlignmentUtils
{
-
private static final int CODON_LENGTH = 3;
private static final String SEQUENCE_VARIANT = "sequence_variant:";
- private static final String ID = "ID";
+ /*
+ * the 'id' attribute is provided for variant features fetched from
+ * Ensembl using its REST service with JSON format
+ */
+ public static final String VARIANT_ID = "id";
/**
* A data model to hold the 'normal' base value at a position, and an optional
{
String lastCodon = String.valueOf(cdnaSeqChars,
cdnaLength - CODON_LENGTH, CODON_LENGTH).toUpperCase();
- for (String stop : ResidueProperties.STOP)
+ for (String stop : ResidueProperties.STOP_CODONS)
{
if (lastCodon.equals(stop))
{
* allow * in protein to match untranslatable in dna
*/
final char aaRes = aaSeqChars[aaPos];
- if ((translated == null || "STOP".equals(translated)) && aaRes == '*')
+ if ((translated == null || ResidueProperties.STOP.equals(translated))
+ && aaRes == '*')
{
continue;
}
if (dnaPos == cdnaSeqChars.length - CODON_LENGTH)
{
String codon = String.valueOf(cdnaSeqChars, dnaPos, CODON_LENGTH);
- if ("STOP".equals(ResidueProperties.codonTranslate(codon)))
+ if (ResidueProperties.STOP
+ .equals(ResidueProperties.codonTranslate(codon)))
{
return true;
}
return;
}
- MapList newMap = targetToFrom.traverse(fromLoci.getMap());
+ MapList newMap = targetToFrom.traverse(fromLoci.getMapping());
if (newMap != null)
{
* @param seqMappings
* the set of mappings involving dnaSeq
* @param aMapping
- * an initial candidate from seqMappings
+ * a transcript-to-peptide mapping
* @return
*/
static SequenceI findCdsForProtein(List<AlignedCodonFrame> mappings,
if (mappedFromLength == dnaLength
|| mappedFromLength == dnaLength - CODON_LENGTH)
{
- return seqDss;
+ /*
+ * if sequence has CDS features, this is a transcript with no UTR
+ * - do not take this as the CDS sequence! (JAL-2789)
+ */
+ if (seqDss.getFeatures().getFeaturesByOntology(SequenceOntologyI.CDS)
+ .isEmpty())
+ {
+ return seqDss;
+ }
}
/*
{
/*
* found a 3:1 mapping to the protein product which covers
- * the whole dna sequence i.e. is from CDS; finally check it
- * is from the dna start sequence
+ * the whole dna sequence i.e. is from CDS; finally check the CDS
+ * is mapped from the given dna start sequence
*/
SequenceI cdsSeq = map.getFromSeq();
+ // todo this test is weak if seqMappings contains multiple mappings;
+ // we get away with it if transcript:cds relationship is 1:1
List<AlignedCodonFrame> dnaToCdsMaps = MappingUtils
.findMappingsForSequence(cdsSeq, seqMappings);
if (!dnaToCdsMaps.isEmpty())
protected static List<DBRefEntry> propagateDBRefsToCDS(SequenceI cdsSeq,
SequenceI contig, SequenceI proteinProduct, Mapping mapping)
{
+
// gather direct refs from contig congruent with mapping
List<DBRefEntry> direct = new ArrayList<>();
HashSet<String> directSources = new HashSet<>();
+
if (contig.getDBRefs() != null)
{
for (DBRefEntry dbr : contig.getDBRefs())
int mappedDnaLength = MappingUtils.getLength(ranges);
/*
- * if not a whole number of codons, something is wrong,
- * abort mapping
+ * if not a whole number of codons, truncate mapping
*/
- if (mappedDnaLength % CODON_LENGTH > 0)
+ int codonRemainder = mappedDnaLength % CODON_LENGTH;
+ if (codonRemainder > 0)
{
- return null;
+ mappedDnaLength -= codonRemainder;
+ MappingUtils.removeEndPositions(codonRemainder, ranges);
}
int proteinLength = proteinSeq.getLength();
{
for (String base : alleles.split(","))
{
- if (!base1.equals(base))
+ if (!base1.equalsIgnoreCase(base))
{
- String codon = base + base2 + base3;
+ String codon = base.toUpperCase() + base2.toLowerCase()
+ + base3.toLowerCase();
+ String canonical = base1.toUpperCase() + base2.toLowerCase()
+ + base3.toLowerCase();
if (addPeptideVariant(peptide, peptidePos, residue, var,
- codon))
+ codon, canonical))
{
count++;
}
{
for (String base : alleles.split(","))
{
- if (!base2.equals(base))
+ if (!base2.equalsIgnoreCase(base))
{
- String codon = base1 + base + base3;
+ String codon = base1.toLowerCase() + base.toUpperCase()
+ + base3.toLowerCase();
+ String canonical = base1.toLowerCase() + base2.toUpperCase()
+ + base3.toLowerCase();
if (addPeptideVariant(peptide, peptidePos, residue, var,
- codon))
+ codon, canonical))
{
count++;
}
{
for (String base : alleles.split(","))
{
- if (!base3.equals(base))
+ if (!base3.equalsIgnoreCase(base))
{
- String codon = base1 + base2 + base;
+ String codon = base1.toLowerCase() + base2.toLowerCase()
+ + base.toUpperCase();
+ String canonical = base1.toLowerCase() + base2.toLowerCase()
+ + base3.toUpperCase();
if (addPeptideVariant(peptide, peptidePos, residue, var,
- codon))
+ codon, canonical))
{
count++;
}
}
/**
- * Helper method that adds a peptide variant feature, provided the given codon
- * translates to a value different to the current residue (is a non-synonymous
- * variant). ID and clinical_significance attributes of the dna variant (if
- * present) are copied to the new feature.
+ * Helper method that adds a peptide variant feature. ID and
+ * clinical_significance attributes of the dna variant (if present) are copied
+ * to the new feature.
*
* @param peptide
* @param peptidePos
* @param residue
* @param var
* @param codon
+ * the variant codon e.g. aCg
+ * @param canonical
+ * the 'normal' codon e.g. aTg
* @return true if a feature was added, else false
*/
static boolean addPeptideVariant(SequenceI peptide, int peptidePos,
- String residue, DnaVariant var, String codon)
+ String residue, DnaVariant var, String codon, String canonical)
{
/*
* get peptide translation of codon e.g. GAT -> D
{
return false;
}
- String desc = codon;
+ String desc = canonical + "/" + codon;
String featureType = "";
if (trans.equals(residue))
{
featureType = SequenceOntologyI.SYNONYMOUS_VARIANT;
}
+ else if (ResidueProperties.STOP.equals(trans))
+ {
+ featureType = SequenceOntologyI.STOP_GAINED;
+ }
else
{
String residue3Char = StringUtils
peptidePos, var.getSource());
StringBuilder attributes = new StringBuilder(32);
- String id = (String) var.variant.getValue(ID);
+ String id = (String) var.variant.getValue(VARIANT_ID);
if (id != null)
{
if (id.startsWith(SEQUENCE_VARIANT))
{
id = id.substring(SEQUENCE_VARIANT.length());
}
- sf.setValue(ID, id);
- attributes.append(ID).append("=").append(id);
+ sf.setValue(VARIANT_ID, id);
+ attributes.append(VARIANT_ID).append("=").append(id);
// TODO handle other species variants JAL-2064
StringBuilder link = new StringBuilder(32);
try
git://source.jalview.org / jalview.git / blobdiff