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+<HEAD>
+<TITLE> New Step 2 Commands</TITLE>
+</HEAD>
+<BODY><P>
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+<HR> <P>
+<H2><A NAME=SECTION00010200000000000000> New Step 2 Commands</A></H2>
+<P>
+COLOUR_TEXT_REGION x y x1 y1 colour
+<P>
+Sets the colour for TEXT command output. Similar syntax to COLOUR_REGION, FONT_REGIONetc.
+<P>
+COLOUR_LINE_REGION x y x1 y1 colour
+<P>
+Set the colour for LINEs in a region.
+<P>
+CALCONS x y x1 y1
+<P>
+Calculate conservation values according to Zvelebil <em>et al.</em> for the designated
+region. (See Livingstone &Barton 1993 for details and further refs)
+<P>
+MASK CONSERVATION cutoff
+<P>
+If CONSCAL has been used, then mask residues according to the conservation cutoff.
+<P>
+e.g. MASK CONSERVATION 10 would mask all identities, MASK CONSERVATION 6 would
+mask reasonably conserved positions. See examples for more on this command.
+<P>
+HELIX x1 y x2
+<P>
+Draw a helix from x1 to x2 of sequence y.
+<P>
+STRAND x1 y x2
+<P>
+Draw a strand from x1 to x2 of sequence y.
+<P>
+COIL x1 y x2
+<P>
+Draw a coil (horizontal line) from x1 to x2 of sequence y.
+<P>
+RELATIVE_TO<seqnum> <startnum>
+<P>
+Set reference numbers to work relative to sequence number <seqnum>. This
+means that in all subsequent commands, ALSCRIPT will translate your x
+values into absolute position values in the alignment. This is <em>extremely</em> useful since you can annotate your alignment using your
+favourite sequences as a reference point. You no longer have to
+translate every x position into the alignment position.
+<P>
+<startnum> is optional. If present, it specifies what the first
+residue in the displayed sequence is. For example, you may be showing
+residues 200-500 of a sequence, so <startnum> would be 200 rather than
+the default of 1. <em>Warning - this is a very new feature and
+bounds checking is not fully enabled for it</em>.
+<P>
+You can use RELATIVE_TOseveral times in the command file to annotate
+different sequences. RELATIVE_TO0 resets to the ``normal''
+alignment numbering.
+<P>
+<HR>
+
+</BODY>
+<P><ADDRESS>
+gjb@bioch.ox.ac.uk
+</ADDRESS>
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