forester/java/src/org/forester/surfacing/SurfacingUtil.java

   1 // $Id:
   2 //
   3 // FORESTER -- software libraries and applications
   4 // for evolutionary biology research and applications.
   5 //
   6 // Copyright (C) 2008-2009 Christian M. Zmasek
   7 // Copyright (C) 2008-2009 Burnham Institute for Medical Research
   8 // All rights reserved
   9 //
  10 // This library is free software; you can redistribute it and/or
  11 // modify it under the terms of the GNU Lesser General Public
  12 // License as published by the Free Software Foundation; either
  13 // version 2.1 of the License, or (at your option) any later version.
  14 //
  15 // This library is distributed in the hope that it will be useful,
  16 // but WITHOUT ANY WARRANTY; without even the implied warranty of
  17 // MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
  18 // Lesser General Public License for more details.
  19 //
  20 // You should have received a copy of the GNU Lesser General Public
  21 // License along with this library; if not, write to the Free Software
  22 // Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA
  23 //
  24 // Contact: phylosoft @ gmail . com
  25 // WWW: www.phylosoft.org/forester
  26
  27 package org.forester.surfacing;
  28
  29 import java.io.BufferedWriter;
  30 import java.io.File;
  31 import java.io.FileWriter;
  32 import java.io.IOException;
  33 import java.io.Writer;
  34 import java.text.DecimalFormat;
  35 import java.text.NumberFormat;
  36 import java.util.ArrayList;
  37 import java.util.Arrays;
  38 import java.util.Collections;
  39 import java.util.Comparator;
  40 import java.util.HashMap;
  41 import java.util.HashSet;
  42 import java.util.List;
  43 import java.util.Map;
  44 import java.util.Map.Entry;
  45 import java.util.PriorityQueue;
  46 import java.util.Set;
  47 import java.util.SortedMap;
  48 import java.util.SortedSet;
  49 import java.util.TreeMap;
  50 import java.util.TreeSet;
  51 import java.util.regex.Matcher;
  52 import java.util.regex.Pattern;
  53
  54 import org.forester.application.surfacing;
  55 import org.forester.evoinference.distance.NeighborJoining;
  56 import org.forester.evoinference.matrix.character.BasicCharacterStateMatrix;
  57 import org.forester.evoinference.matrix.character.CharacterStateMatrix;
  58 import org.forester.evoinference.matrix.character.CharacterStateMatrix.BinaryStates;
  59 import org.forester.evoinference.matrix.character.CharacterStateMatrix.Format;
  60 import org.forester.evoinference.matrix.character.CharacterStateMatrix.GainLossStates;
  61 import org.forester.evoinference.matrix.distance.BasicSymmetricalDistanceMatrix;
  62 import org.forester.evoinference.matrix.distance.DistanceMatrix;
  63 import org.forester.go.GoId;
  64 import org.forester.go.GoNameSpace;
  65 import org.forester.go.GoTerm;
  66 import org.forester.go.PfamToGoMapping;
  67 import org.forester.io.parsers.nexus.NexusConstants;
  68 import org.forester.io.writers.PhylogenyWriter;
  69 import org.forester.msa.MsaCompactor.SORT_BY;
  70 import org.forester.phylogeny.Phylogeny;
  71 import org.forester.phylogeny.PhylogenyMethods;
  72 import org.forester.phylogeny.PhylogenyNode;
  73 import org.forester.phylogeny.PhylogenyNodeI.NH_CONVERSION_SUPPORT_VALUE_STYLE;
  74 import org.forester.phylogeny.data.BinaryCharacters;
  75 import org.forester.phylogeny.data.Confidence;
  76 import org.forester.phylogeny.iterators.PhylogenyNodeIterator;
  77 import org.forester.protein.BasicDomain;
  78 import org.forester.protein.BasicProtein;
  79 import org.forester.protein.BinaryDomainCombination;
  80 import org.forester.protein.Domain;
  81 import org.forester.protein.DomainId;
  82 import org.forester.protein.Protein;
  83 import org.forester.species.Species;
  84 import org.forester.surfacing.DomainSimilarityCalculator.Detailedness;
  85 import org.forester.surfacing.DomainSimilarityCalculator.GoAnnotationOutput;
  86 import org.forester.surfacing.GenomeWideCombinableDomains.GenomeWideCombinableDomainsSortOrder;
  87 import org.forester.util.AsciiHistogram;
  88 import org.forester.util.BasicDescriptiveStatistics;
  89 import org.forester.util.BasicTable;
  90 import org.forester.util.BasicTableParser;
  91 import org.forester.util.DescriptiveStatistics;
  92 import org.forester.util.ForesterUtil;
  93
  94 public final class SurfacingUtil {
  95
  96     private final static NumberFormat       FORMATTER                        = new DecimalFormat( "0.0E0" );
  97     private final static NumberFormat       FORMATTER_3                      = new DecimalFormat( "0.000" );
  98     private static final Comparator<Domain> ASCENDING_CONFIDENCE_VALUE_ORDER = new Comparator<Domain>() {
  99
 100                                                                                  @Override
 101                                                                                  public int compare( final Domain d1,
 102                                                                                                      final Domain d2 ) {
 103                                                                                      if ( d1.getPerSequenceEvalue() < d2
 104                                                                                              .getPerSequenceEvalue() ) {
 105                                                                                          return -1;
 106                                                                                      }
 107                                                                                      else if ( d1
 108                                                                                              .getPerSequenceEvalue() > d2
 109                                                                                              .getPerSequenceEvalue() ) {
 110                                                                                          return 1;
 111                                                                                      }
 112                                                                                      else {
 113                                                                                          return d1.compareTo( d2 );
 114                                                                                      }
 115                                                                                  }
 116                                                                              };
 117     public final static Pattern             PATTERN_SP_STYLE_TAXONOMY        = Pattern.compile( "^[A-Z0-9]{3,5}$" );
 118     private static final boolean            USE_LAST                         = true;
 119
 120     private SurfacingUtil() {
 121         // Hidden constructor.
 122     }
 123
 124     public static void addAllBinaryDomainCombinationToSet( final GenomeWideCombinableDomains genome,
 125                                                            final SortedSet<BinaryDomainCombination> binary_domain_combinations ) {
 126         final SortedMap<DomainId, CombinableDomains> all_cd = genome.getAllCombinableDomainsIds();
 127         for( final DomainId domain_id : all_cd.keySet() ) {
 128             binary_domain_combinations.addAll( all_cd.get( domain_id ).toBinaryDomainCombinations() );
 129         }
 130     }
 131
 132     public static void addAllDomainIdsToSet( final GenomeWideCombinableDomains genome,
 133                                              final SortedSet<DomainId> domain_ids ) {
 134         final SortedSet<DomainId> domains = genome.getAllDomainIds();
 135         for( final DomainId domain : domains ) {
 136             domain_ids.add( domain );
 137         }
 138     }
 139
 140     public static void addHtmlHead( final Writer w, final String title ) throws IOException {
 141         w.write( SurfacingConstants.NL );
 142         w.write( "<head>" );
 143         w.write( "<title>" );
 144         w.write( title );
 145         w.write( "</title>" );
 146         w.write( SurfacingConstants.NL );
 147         w.write( "<style>" );
 148         w.write( SurfacingConstants.NL );
 149         w.write( "a:visited { color : #6633FF; text-decoration : none; }" );
 150         w.write( SurfacingConstants.NL );
 151         w.write( "a:link { color : #6633FF; text-decoration : none; }" );
 152         w.write( SurfacingConstants.NL );
 153         w.write( "a:active { color : #99FF00; text-decoration : none; }" );
 154         w.write( SurfacingConstants.NL );
 155         w.write( "a:hover { color : #FFFFFF; background-color : #99FF00; text-decoration : none; }" );
 156         w.write( SurfacingConstants.NL );
 157         w.write( "td { text-align: left; vertical-align: top; font-family: Verdana, Arial, Helvetica; font-size: 8pt}" );
 158         w.write( SurfacingConstants.NL );
 159         w.write( "h1 { color : #0000FF; font-family: Verdana, Arial, Helvetica; font-size: 18pt; font-weight: bold }" );
 160         w.write( SurfacingConstants.NL );
 161         w.write( "h2 { color : #0000FF; font-family: Verdana, Arial, Helvetica; font-size: 16pt; font-weight: bold }" );
 162         w.write( SurfacingConstants.NL );
 163         w.write( "</style>" );
 164         w.write( SurfacingConstants.NL );
 165         w.write( "</head>" );
 166         w.write( SurfacingConstants.NL );
 167     }
 168
 169     public static DescriptiveStatistics calculateDescriptiveStatisticsForMeanValues( final Set<DomainSimilarity> similarities ) {
 170         final DescriptiveStatistics stats = new BasicDescriptiveStatistics();
 171         for( final DomainSimilarity similarity : similarities ) {
 172             stats.addValue( similarity.getMeanSimilarityScore() );
 173         }
 174         return stats;
 175     }
 176
 177     private static void calculateIndependentDomainCombinationGains( final Phylogeny local_phylogeny_l,
 178                                                                     final String outfilename_for_counts,
 179                                                                     final String outfilename_for_dc,
 180                                                                     final String outfilename_for_dc_for_go_mapping,
 181                                                                     final String outfilename_for_dc_for_go_mapping_unique,
 182                                                                     final String outfilename_for_rank_counts,
 183                                                                     final String outfilename_for_ancestor_species_counts,
 184                                                                     final String outfilename_for_protein_stats,
 185                                                                     final Map<String, DescriptiveStatistics> protein_length_stats_by_dc,
 186                                                                     final Map<String, DescriptiveStatistics> domain_number_stats_by_dc,
 187                                                                     final Map<String, DescriptiveStatistics> domain_length_stats_by_domain ) {
 188         try {
 189             //
 190             //            if ( protein_length_stats_by_dc != null ) {
 191             //                for( final Entry<?, DescriptiveStatistics> entry : protein_length_stats_by_dc.entrySet() ) {
 192             //                    System.out.print( entry.getKey().toString() );
 193             //                    System.out.print( ": " );
 194             //                    double[] a = entry.getValue().getDataAsDoubleArray();
 195             //                    for( int i = 0; i < a.length; i++ ) {
 196             //                        System.out.print( a[ i ] + " " );
 197             //                    }
 198             //                    System.out.println();
 199             //                }
 200             //            }
 201             //            if ( domain_number_stats_by_dc != null ) {
 202             //                for( final Entry<?, DescriptiveStatistics> entry : domain_number_stats_by_dc.entrySet() ) {
 203             //                    System.out.print( entry.getKey().toString() );
 204             //                    System.out.print( ": " );
 205             //                    double[] a = entry.getValue().getDataAsDoubleArray();
 206             //                    for( int i = 0; i < a.length; i++ ) {
 207             //                        System.out.print( a[ i ] + " " );
 208             //                    }
 209             //                    System.out.println();
 210             //                }
 211             //            }
 212             //
 213             final BufferedWriter out_counts = new BufferedWriter( new FileWriter( outfilename_for_counts ) );
 214             final BufferedWriter out_dc = new BufferedWriter( new FileWriter( outfilename_for_dc ) );
 215             final BufferedWriter out_dc_for_go_mapping = new BufferedWriter( new FileWriter( outfilename_for_dc_for_go_mapping ) );
 216             final BufferedWriter out_dc_for_go_mapping_unique = new BufferedWriter( new FileWriter( outfilename_for_dc_for_go_mapping_unique ) );
 217             final SortedMap<String, Integer> dc_gain_counts = new TreeMap<String, Integer>();
 218             for( final PhylogenyNodeIterator it = local_phylogeny_l.iteratorPostorder(); it.hasNext(); ) {
 219                 final PhylogenyNode n = it.next();
 220                 final Set<String> gained_dc = n.getNodeData().getBinaryCharacters().getGainedCharacters();
 221                 for( final String dc : gained_dc ) {
 222                     if ( dc_gain_counts.containsKey( dc ) ) {
 223                         dc_gain_counts.put( dc, dc_gain_counts.get( dc ) + 1 );
 224                     }
 225                     else {
 226                         dc_gain_counts.put( dc, 1 );
 227                     }
 228                 }
 229             }
 230             final SortedMap<Integer, Integer> histogram = new TreeMap<Integer, Integer>();
 231             final SortedMap<Integer, StringBuilder> domain_lists = new TreeMap<Integer, StringBuilder>();
 232             final SortedMap<Integer, DescriptiveStatistics> dc_reapp_counts_to_protein_length_stats = new TreeMap<Integer, DescriptiveStatistics>();
 233             final SortedMap<Integer, DescriptiveStatistics> dc_reapp_counts_to_domain_number_stats = new TreeMap<Integer, DescriptiveStatistics>();
 234             final SortedMap<Integer, DescriptiveStatistics> dc_reapp_counts_to_domain_lengths_stats = new TreeMap<Integer, DescriptiveStatistics>();
 235             final SortedMap<Integer, PriorityQueue<String>> domain_lists_go = new TreeMap<Integer, PriorityQueue<String>>();
 236             final SortedMap<Integer, SortedSet<String>> domain_lists_go_unique = new TreeMap<Integer, SortedSet<String>>();
 237             final Set<String> dcs = dc_gain_counts.keySet();
 238             final SortedSet<String> more_than_once = new TreeSet<String>();
 239             final DescriptiveStatistics gained_once_lengths_stats = new BasicDescriptiveStatistics();
 240             final DescriptiveStatistics gained_once_domain_count_stats = new BasicDescriptiveStatistics();
 241             final DescriptiveStatistics gained_multiple_times_lengths_stats = new BasicDescriptiveStatistics();
 242             final DescriptiveStatistics gained_multiple_times_domain_count_stats = new BasicDescriptiveStatistics();
 243             long gained_multiple_times_domain_length_sum = 0;
 244             long gained_once_domain_length_sum = 0;
 245             long gained_multiple_times_domain_length_count = 0;
 246             long gained_once_domain_length_count = 0;
 247             for( final String dc : dcs ) {
 248                 final int count = dc_gain_counts.get( dc );
 249                 if ( histogram.containsKey( count ) ) {
 250                     histogram.put( count, histogram.get( count ) + 1 );
 251                     domain_lists.get( count ).append( ", " + dc );
 252                     domain_lists_go.get( count ).addAll( splitDomainCombination( dc ) );
 253                     domain_lists_go_unique.get( count ).addAll( splitDomainCombination( dc ) );
 254                 }
 255                 else {
 256                     histogram.put( count, 1 );
 257                     domain_lists.put( count, new StringBuilder( dc ) );
 258                     final PriorityQueue<String> q = new PriorityQueue<String>();
 259                     q.addAll( splitDomainCombination( dc ) );
 260                     domain_lists_go.put( count, q );
 261                     final SortedSet<String> set = new TreeSet<String>();
 262                     set.addAll( splitDomainCombination( dc ) );
 263                     domain_lists_go_unique.put( count, set );
 264                 }
 265                 if ( protein_length_stats_by_dc != null ) {
 266                     if ( !dc_reapp_counts_to_protein_length_stats.containsKey( count ) ) {
 267                         dc_reapp_counts_to_protein_length_stats.put( count, new BasicDescriptiveStatistics() );
 268                     }
 269                     dc_reapp_counts_to_protein_length_stats.get( count ).addValue( protein_length_stats_by_dc.get( dc )
 270                             .arithmeticMean() );
 271                 }
 272                 if ( domain_number_stats_by_dc != null ) {
 273                     if ( !dc_reapp_counts_to_domain_number_stats.containsKey( count ) ) {
 274                         dc_reapp_counts_to_domain_number_stats.put( count, new BasicDescriptiveStatistics() );
 275                     }
 276                     dc_reapp_counts_to_domain_number_stats.get( count ).addValue( domain_number_stats_by_dc.get( dc )
 277                             .arithmeticMean() );
 278                 }
 279                 if ( domain_length_stats_by_domain != null ) {
 280                     if ( !dc_reapp_counts_to_domain_lengths_stats.containsKey( count ) ) {
 281                         dc_reapp_counts_to_domain_lengths_stats.put( count, new BasicDescriptiveStatistics() );
 282                     }
 283                     final String[] ds = dc.split( "=" );
 284                     dc_reapp_counts_to_domain_lengths_stats.get( count ).addValue( domain_length_stats_by_domain
 285                             .get( ds[ 0 ] ).arithmeticMean() );
 286                     dc_reapp_counts_to_domain_lengths_stats.get( count ).addValue( domain_length_stats_by_domain
 287                             .get( ds[ 1 ] ).arithmeticMean() );
 288                 }
 289                 if ( count > 1 ) {
 290                     more_than_once.add( dc );
 291                     if ( protein_length_stats_by_dc != null ) {
 292                         final DescriptiveStatistics s = protein_length_stats_by_dc.get( dc );
 293                         for( final double element : s.getData() ) {
 294                             gained_multiple_times_lengths_stats.addValue( element );
 295                         }
 296                     }
 297                     if ( domain_number_stats_by_dc != null ) {
 298                         final DescriptiveStatistics s = domain_number_stats_by_dc.get( dc );
 299                         for( final double element : s.getData() ) {
 300                             gained_multiple_times_domain_count_stats.addValue( element );
 301                         }
 302                     }
 303                     if ( domain_length_stats_by_domain != null ) {
 304                         final String[] ds = dc.split( "=" );
 305                         final DescriptiveStatistics s0 = domain_length_stats_by_domain.get( ds[ 0 ] );
 306                         final DescriptiveStatistics s1 = domain_length_stats_by_domain.get( ds[ 1 ] );
 307                         for( final double element : s0.getData() ) {
 308                             gained_multiple_times_domain_length_sum += element;
 309                             ++gained_multiple_times_domain_length_count;
 310                         }
 311                         for( final double element : s1.getData() ) {
 312                             gained_multiple_times_domain_length_sum += element;
 313                             ++gained_multiple_times_domain_length_count;
 314                         }
 315                     }
 316                 }
 317                 else {
 318                     if ( protein_length_stats_by_dc != null ) {
 319                         final DescriptiveStatistics s = protein_length_stats_by_dc.get( dc );
 320                         for( final double element : s.getData() ) {
 321                             gained_once_lengths_stats.addValue( element );
 322                         }
 323                     }
 324                     if ( domain_number_stats_by_dc != null ) {
 325                         final DescriptiveStatistics s = domain_number_stats_by_dc.get( dc );
 326                         for( final double element : s.getData() ) {
 327                             gained_once_domain_count_stats.addValue( element );
 328                         }
 329                     }
 330                     if ( domain_length_stats_by_domain != null ) {
 331                         final String[] ds = dc.split( "=" );
 332                         final DescriptiveStatistics s0 = domain_length_stats_by_domain.get( ds[ 0 ] );
 333                         final DescriptiveStatistics s1 = domain_length_stats_by_domain.get( ds[ 1 ] );
 334                         for( final double element : s0.getData() ) {
 335                             gained_once_domain_length_sum += element;
 336                             ++gained_once_domain_length_count;
 337                         }
 338                         for( final double element : s1.getData() ) {
 339                             gained_once_domain_length_sum += element;
 340                             ++gained_once_domain_length_count;
 341                         }
 342                     }
 343                 }
 344             }
 345             final Set<Integer> histogram_keys = histogram.keySet();
 346             for( final Integer histogram_key : histogram_keys ) {
 347                 final int count = histogram.get( histogram_key );
 348                 final StringBuilder dc = domain_lists.get( histogram_key );
 349                 out_counts.write( histogram_key + "\t" + count + ForesterUtil.LINE_SEPARATOR );
 350                 out_dc.write( histogram_key + "\t" + dc + ForesterUtil.LINE_SEPARATOR );
 351                 out_dc_for_go_mapping.write( "#" + histogram_key + ForesterUtil.LINE_SEPARATOR );
 352                 final Object[] sorted = domain_lists_go.get( histogram_key ).toArray();
 353                 Arrays.sort( sorted );
 354                 for( final Object domain : sorted ) {
 355                     out_dc_for_go_mapping.write( domain + ForesterUtil.LINE_SEPARATOR );
 356                 }
 357                 out_dc_for_go_mapping_unique.write( "#" + histogram_key + ForesterUtil.LINE_SEPARATOR );
 358                 for( final String domain : domain_lists_go_unique.get( histogram_key ) ) {
 359                     out_dc_for_go_mapping_unique.write( domain + ForesterUtil.LINE_SEPARATOR );
 360                 }
 361             }
 362             out_counts.close();
 363             out_dc.close();
 364             out_dc_for_go_mapping.close();
 365             out_dc_for_go_mapping_unique.close();
 366             final SortedMap<String, Integer> lca_rank_counts = new TreeMap<String, Integer>();
 367             final SortedMap<String, Integer> lca_ancestor_species_counts = new TreeMap<String, Integer>();
 368             for( final String dc : more_than_once ) {
 369                 final List<PhylogenyNode> nodes = new ArrayList<PhylogenyNode>();
 370                 for( final PhylogenyNodeIterator it = local_phylogeny_l.iteratorExternalForward(); it.hasNext(); ) {
 371                     final PhylogenyNode n = it.next();
 372                     if ( n.getNodeData().getBinaryCharacters().getGainedCharacters().contains( dc ) ) {
 373                         nodes.add( n );
 374                     }
 375                 }
 376                 for( int i = 0; i < nodes.size() - 1; ++i ) {
 377                     for( int j = i + 1; j < nodes.size(); ++j ) {
 378                         final PhylogenyNode lca = PhylogenyMethods.obtainLCA( nodes.get( i ),
 379                                                                                             nodes.get( j ) );
 380                         String rank = "unknown";
 381                         if ( lca.getNodeData().isHasTaxonomy()
 382                                 && !ForesterUtil.isEmpty( lca.getNodeData().getTaxonomy().getRank() ) ) {
 383                             rank = lca.getNodeData().getTaxonomy().getRank();
 384                         }
 385                         addToCountMap( lca_rank_counts, rank );
 386                         String lca_species;
 387                         if ( lca.getNodeData().isHasTaxonomy()
 388                                 && !ForesterUtil.isEmpty( lca.getNodeData().getTaxonomy().getScientificName() ) ) {
 389                             lca_species = lca.getNodeData().getTaxonomy().getScientificName();
 390                         }
 391                         else if ( lca.getNodeData().isHasTaxonomy()
 392                                 && !ForesterUtil.isEmpty( lca.getNodeData().getTaxonomy().getCommonName() ) ) {
 393                             lca_species = lca.getNodeData().getTaxonomy().getCommonName();
 394                         }
 395                         else {
 396                             lca_species = lca.getName();
 397                         }
 398                         addToCountMap( lca_ancestor_species_counts, lca_species );
 399                     }
 400                 }
 401             }
 402             final BufferedWriter out_for_rank_counts = new BufferedWriter( new FileWriter( outfilename_for_rank_counts ) );
 403             final BufferedWriter out_for_ancestor_species_counts = new BufferedWriter( new FileWriter( outfilename_for_ancestor_species_counts ) );
 404             ForesterUtil.map2writer( out_for_rank_counts, lca_rank_counts, "\t", ForesterUtil.LINE_SEPARATOR );
 405             ForesterUtil.map2writer( out_for_ancestor_species_counts,
 406                                      lca_ancestor_species_counts,
 407                                      "\t",
 408                                      ForesterUtil.LINE_SEPARATOR );
 409             out_for_rank_counts.close();
 410             out_for_ancestor_species_counts.close();
 411             if ( !ForesterUtil.isEmpty( outfilename_for_protein_stats )
 412                     && ( ( domain_length_stats_by_domain != null ) || ( protein_length_stats_by_dc != null ) || ( domain_number_stats_by_dc != null ) ) ) {
 413                 final BufferedWriter w = new BufferedWriter( new FileWriter( outfilename_for_protein_stats ) );
 414                 w.write( "Domain Lengths: " );
 415                 w.write( "\n" );
 416                 if ( domain_length_stats_by_domain != null ) {
 417                     for( final Entry<Integer, DescriptiveStatistics> entry : dc_reapp_counts_to_domain_lengths_stats
 418                             .entrySet() ) {
 419                         w.write( entry.getKey().toString() );
 420                         w.write( "\t" + entry.getValue().arithmeticMean() );
 421                         w.write( "\t" + entry.getValue().median() );
 422                         w.write( "\n" );
 423                     }
 424                 }
 425                 w.flush();
 426                 w.write( "\n" );
 427                 w.write( "\n" );
 428                 w.write( "Protein Lengths: " );
 429                 w.write( "\n" );
 430                 if ( protein_length_stats_by_dc != null ) {
 431                     for( final Entry<Integer, DescriptiveStatistics> entry : dc_reapp_counts_to_protein_length_stats
 432                             .entrySet() ) {
 433                         w.write( entry.getKey().toString() );
 434                         w.write( "\t" + entry.getValue().arithmeticMean() );
 435                         w.write( "\t" + entry.getValue().median() );
 436                         w.write( "\n" );
 437                     }
 438                 }
 439                 w.flush();
 440                 w.write( "\n" );
 441                 w.write( "\n" );
 442                 w.write( "Number of domains: " );
 443                 w.write( "\n" );
 444                 if ( domain_number_stats_by_dc != null ) {
 445                     for( final Entry<Integer, DescriptiveStatistics> entry : dc_reapp_counts_to_domain_number_stats
 446                             .entrySet() ) {
 447                         w.write( entry.getKey().toString() );
 448                         w.write( "\t" + entry.getValue().arithmeticMean() );
 449                         w.write( "\t" + entry.getValue().median() );
 450                         w.write( "\n" );
 451                     }
 452                 }
 453                 w.flush();
 454                 w.write( "\n" );
 455                 w.write( "\n" );
 456                 w.write( "Gained once, domain lengths:" );
 457                 w.write( "\n" );
 458                 w.write( "N: " + gained_once_domain_length_count );
 459                 w.write( "\n" );
 460                 w.write( "Avg: " + ( ( double ) gained_once_domain_length_sum / gained_once_domain_length_count ) );
 461                 w.write( "\n" );
 462                 w.write( "\n" );
 463                 w.write( "Gained multiple times, domain lengths:" );
 464                 w.write( "\n" );
 465                 w.write( "N: " + gained_multiple_times_domain_length_count );
 466                 w.write( "\n" );
 467                 w.write( "Avg: "
 468                         + ( ( double ) gained_multiple_times_domain_length_sum / gained_multiple_times_domain_length_count ) );
 469                 w.write( "\n" );
 470                 w.write( "\n" );
 471                 w.write( "\n" );
 472                 w.write( "\n" );
 473                 w.write( "Gained once, protein lengths:" );
 474                 w.write( "\n" );
 475                 w.write( gained_once_lengths_stats.toString() );
 476                 w.write( "\n" );
 477                 w.write( "\n" );
 478                 w.write( "Gained once, domain counts:" );
 479                 w.write( "\n" );
 480                 w.write( gained_once_domain_count_stats.toString() );
 481                 w.write( "\n" );
 482                 w.write( "\n" );
 483                 w.write( "Gained multiple times, protein lengths:" );
 484                 w.write( "\n" );
 485                 w.write( gained_multiple_times_lengths_stats.toString() );
 486                 w.write( "\n" );
 487                 w.write( "\n" );
 488                 w.write( "Gained multiple times, domain counts:" );
 489                 w.write( "\n" );
 490                 w.write( gained_multiple_times_domain_count_stats.toString() );
 491                 w.flush();
 492                 w.close();
 493             }
 494         }
 495         catch ( final IOException e ) {
 496             ForesterUtil.printWarningMessage( surfacing.PRG_NAME, "Failure to write: " + e );
 497         }
 498         ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote independent domain combination gains fitch counts to ["
 499                 + outfilename_for_counts + "]" );
 500         ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote independent domain combination gains fitch lists to ["
 501                 + outfilename_for_dc + "]" );
 502         ForesterUtil.programMessage( surfacing.PRG_NAME,
 503                                      "Wrote independent domain combination gains fitch lists to (for GO mapping) ["
 504                                              + outfilename_for_dc_for_go_mapping + "]" );
 505         ForesterUtil.programMessage( surfacing.PRG_NAME,
 506                                      "Wrote independent domain combination gains fitch lists to (for GO mapping, unique) ["
 507                                              + outfilename_for_dc_for_go_mapping_unique + "]" );
 508     }
 509
 510     private final static void addToCountMap( final Map<String, Integer> map, final String s ) {
 511         if ( map.containsKey( s ) ) {
 512             map.put( s, map.get( s ) + 1 );
 513         }
 514         else {
 515             map.put( s, 1 );
 516         }
 517     }
 518
 519     public static int calculateOverlap( final Domain domain, final List<Boolean> covered_positions ) {
 520         int overlap_count = 0;
 521         for( int i = domain.getFrom(); i <= domain.getTo(); ++i ) {
 522             if ( ( i < covered_positions.size() ) && ( covered_positions.get( i ) == true ) ) {
 523                 ++overlap_count;
 524             }
 525         }
 526         return overlap_count;
 527     }
 528
 529     public static void checkForOutputFileWriteability( final File outfile ) {
 530         final String error = ForesterUtil.isWritableFile( outfile );
 531         if ( !ForesterUtil.isEmpty( error ) ) {
 532             ForesterUtil.fatalError( surfacing.PRG_NAME, error );
 533         }
 534     }
 535
 536     private static SortedSet<String> collectAllDomainsChangedOnSubtree( final PhylogenyNode subtree_root,
 537                                                                         final boolean get_gains ) {
 538         final SortedSet<String> domains = new TreeSet<String>();
 539         for( final PhylogenyNode descendant : PhylogenyMethods.getAllDescendants( subtree_root ) ) {
 540             final BinaryCharacters chars = descendant.getNodeData().getBinaryCharacters();
 541             if ( get_gains ) {
 542                 domains.addAll( chars.getGainedCharacters() );
 543             }
 544             else {
 545                 domains.addAll( chars.getLostCharacters() );
 546             }
 547         }
 548         return domains;
 549     }
 550
 551     public static void collectChangedDomainCombinationsFromBinaryStatesMatrixAsListToFile( final CharacterStateMatrix<CharacterStateMatrix.GainLossStates> matrix,
 552                                                                                            final BinaryDomainCombination.DomainCombinationType dc_type,
 553                                                                                            final List<BinaryDomainCombination> all_binary_domains_combination_gained,
 554                                                                                            final boolean get_gains ) {
 555         final SortedSet<String> sorted_ids = new TreeSet<String>();
 556         for( int i = 0; i < matrix.getNumberOfIdentifiers(); ++i ) {
 557             sorted_ids.add( matrix.getIdentifier( i ) );
 558         }
 559         for( final String id : sorted_ids ) {
 560             for( int c = 0; c < matrix.getNumberOfCharacters(); ++c ) {
 561                 if ( ( get_gains && ( matrix.getState( id, c ) == CharacterStateMatrix.GainLossStates.GAIN ) )
 562                         || ( !get_gains && ( matrix.getState( id, c ) == CharacterStateMatrix.GainLossStates.LOSS ) ) ) {
 563                     if ( dc_type == BinaryDomainCombination.DomainCombinationType.DIRECTED_ADJACTANT ) {
 564                         all_binary_domains_combination_gained.add( AdjactantDirectedBinaryDomainCombination
 565                                 .createInstance( matrix.getCharacter( c ) ) );
 566                     }
 567                     else if ( dc_type == BinaryDomainCombination.DomainCombinationType.DIRECTED ) {
 568                         all_binary_domains_combination_gained.add( DirectedBinaryDomainCombination
 569                                 .createInstance( matrix.getCharacter( c ) ) );
 570                     }
 571                     else {
 572                         all_binary_domains_combination_gained.add( BasicBinaryDomainCombination.createInstance( matrix
 573                                 .getCharacter( c ) ) );
 574                     }
 575                 }
 576             }
 577         }
 578     }
 579
 580     private static File createBaseDirForPerNodeDomainFiles( final String base_dir,
 581                                                             final boolean domain_combinations,
 582                                                             final CharacterStateMatrix.GainLossStates state,
 583                                                             final String outfile ) {
 584         File per_node_go_mapped_domain_gain_loss_files_base_dir = new File( new File( outfile ).getParent()
 585                 + ForesterUtil.FILE_SEPARATOR + base_dir );
 586         if ( !per_node_go_mapped_domain_gain_loss_files_base_dir.exists() ) {
 587             per_node_go_mapped_domain_gain_loss_files_base_dir.mkdir();
 588         }
 589         if ( domain_combinations ) {
 590             per_node_go_mapped_domain_gain_loss_files_base_dir = new File( per_node_go_mapped_domain_gain_loss_files_base_dir
 591                     + ForesterUtil.FILE_SEPARATOR + "DC" );
 592         }
 593         else {
 594             per_node_go_mapped_domain_gain_loss_files_base_dir = new File( per_node_go_mapped_domain_gain_loss_files_base_dir
 595                     + ForesterUtil.FILE_SEPARATOR + "DOMAINS" );
 596         }
 597         if ( !per_node_go_mapped_domain_gain_loss_files_base_dir.exists() ) {
 598             per_node_go_mapped_domain_gain_loss_files_base_dir.mkdir();
 599         }
 600         if ( state == GainLossStates.GAIN ) {
 601             per_node_go_mapped_domain_gain_loss_files_base_dir = new File( per_node_go_mapped_domain_gain_loss_files_base_dir
 602                     + ForesterUtil.FILE_SEPARATOR + "GAINS" );
 603         }
 604         else if ( state == GainLossStates.LOSS ) {
 605             per_node_go_mapped_domain_gain_loss_files_base_dir = new File( per_node_go_mapped_domain_gain_loss_files_base_dir
 606                     + ForesterUtil.FILE_SEPARATOR + "LOSSES" );
 607         }
 608         else {
 609             per_node_go_mapped_domain_gain_loss_files_base_dir = new File( per_node_go_mapped_domain_gain_loss_files_base_dir
 610                     + ForesterUtil.FILE_SEPARATOR + "PRESENT" );
 611         }
 612         if ( !per_node_go_mapped_domain_gain_loss_files_base_dir.exists() ) {
 613             per_node_go_mapped_domain_gain_loss_files_base_dir.mkdir();
 614         }
 615         return per_node_go_mapped_domain_gain_loss_files_base_dir;
 616     }
 617
 618     public static Map<DomainId, List<GoId>> createDomainIdToGoIdMap( final List<PfamToGoMapping> pfam_to_go_mappings ) {
 619         final Map<DomainId, List<GoId>> domain_id_to_go_ids_map = new HashMap<DomainId, List<GoId>>( pfam_to_go_mappings
 620                 .size() );
 621         for( final PfamToGoMapping pfam_to_go : pfam_to_go_mappings ) {
 622             if ( !domain_id_to_go_ids_map.containsKey( pfam_to_go.getKey() ) ) {
 623                 domain_id_to_go_ids_map.put( pfam_to_go.getKey(), new ArrayList<GoId>() );
 624             }
 625             domain_id_to_go_ids_map.get( pfam_to_go.getKey() ).add( pfam_to_go.getValue() );
 626         }
 627         return domain_id_to_go_ids_map;
 628     }
 629
 630     public static Map<DomainId, Set<String>> createDomainIdToSecondaryFeaturesMap( final File secondary_features_map_file )
 631             throws IOException {
 632         final BasicTable<String> primary_table = BasicTableParser.parse( secondary_features_map_file, "\t" );
 633         final Map<DomainId, Set<String>> map = new TreeMap<DomainId, Set<String>>();
 634         for( int r = 0; r < primary_table.getNumberOfRows(); ++r ) {
 635             final DomainId domain_id = new DomainId( primary_table.getValue( 0, r ) );
 636             if ( !map.containsKey( domain_id ) ) {
 637                 map.put( domain_id, new HashSet<String>() );
 638             }
 639             map.get( domain_id ).add( primary_table.getValue( 1, r ) );
 640         }
 641         return map;
 642     }
 643
 644     public static Phylogeny createNjTreeBasedOnMatrixToFile( final File nj_tree_outfile, final DistanceMatrix distance ) {
 645         checkForOutputFileWriteability( nj_tree_outfile );
 646         final NeighborJoining nj = NeighborJoining.createInstance();
 647         final Phylogeny phylogeny = nj.execute( ( BasicSymmetricalDistanceMatrix ) distance );
 648         phylogeny.setName( nj_tree_outfile.getName() );
 649         writePhylogenyToFile( phylogeny, nj_tree_outfile.toString() );
 650         return phylogeny;
 651     }
 652
 653     private static SortedSet<BinaryDomainCombination> createSetOfAllBinaryDomainCombinationsPerGenome( final GenomeWideCombinableDomains gwcd ) {
 654         final SortedMap<DomainId, CombinableDomains> cds = gwcd.getAllCombinableDomainsIds();
 655         final SortedSet<BinaryDomainCombination> binary_combinations = new TreeSet<BinaryDomainCombination>();
 656         for( final DomainId domain_id : cds.keySet() ) {
 657             final CombinableDomains cd = cds.get( domain_id );
 658             binary_combinations.addAll( cd.toBinaryDomainCombinations() );
 659         }
 660         return binary_combinations;
 661     }
 662
 663     public static void decoratePrintableDomainSimilarities( final SortedSet<DomainSimilarity> domain_similarities,
 664                                                             final Detailedness detailedness,
 665                                                             final GoAnnotationOutput go_annotation_output,
 666                                                             final Map<GoId, GoTerm> go_id_to_term_map,
 667                                                             final GoNameSpace go_namespace_limit ) {
 668         if ( ( go_namespace_limit != null ) && ( ( go_id_to_term_map == null ) || go_id_to_term_map.isEmpty() ) ) {
 669             throw new IllegalArgumentException( "attempt to use a GO namespace limit without a GO id to term map" );
 670         }
 671         for( final DomainSimilarity domain_similarity : domain_similarities ) {
 672             if ( domain_similarity instanceof PrintableDomainSimilarity ) {
 673                 final PrintableDomainSimilarity printable_domain_similarity = ( PrintableDomainSimilarity ) domain_similarity;
 674                 printable_domain_similarity.setDetailedness( detailedness );
 675                 printable_domain_similarity.setGoAnnotationOutput( go_annotation_output );
 676                 printable_domain_similarity.setGoIdToTermMap( go_id_to_term_map );
 677                 printable_domain_similarity.setGoNamespaceLimit( go_namespace_limit );
 678             }
 679         }
 680     }
 681
 682     public static void executeDomainLengthAnalysis( final String[][] input_file_properties,
 683                                                     final int number_of_genomes,
 684                                                     final DomainLengthsTable domain_lengths_table,
 685                                                     final File outfile ) throws IOException {
 686         final DecimalFormat df = new DecimalFormat( "#.00" );
 687         checkForOutputFileWriteability( outfile );
 688         final BufferedWriter out = new BufferedWriter( new FileWriter( outfile ) );
 689         out.write( "MEAN BASED STATISTICS PER SPECIES" );
 690         out.write( ForesterUtil.LINE_SEPARATOR );
 691         out.write( domain_lengths_table.createMeanBasedStatisticsPerSpeciesTable().toString() );
 692         out.write( ForesterUtil.LINE_SEPARATOR );
 693         out.write( ForesterUtil.LINE_SEPARATOR );
 694         final List<DomainLengths> domain_lengths_list = domain_lengths_table.getDomainLengthsList();
 695         out.write( "OUTLIER SPECIES PER DOMAIN (Z>=1.5)" );
 696         out.write( ForesterUtil.LINE_SEPARATOR );
 697         for( final DomainLengths domain_lengths : domain_lengths_list ) {
 698             final List<Species> species_list = domain_lengths.getMeanBasedOutlierSpecies( 1.5 );
 699             if ( species_list.size() > 0 ) {
 700                 out.write( domain_lengths.getDomainId() + "\t" );
 701                 for( final Species species : species_list ) {
 702                     out.write( species + "\t" );
 703                 }
 704                 out.write( ForesterUtil.LINE_SEPARATOR );
 705                 // DescriptiveStatistics stats_for_domain = domain_lengths
 706                 //         .calculateMeanBasedStatistics();
 707                 //AsciiHistogram histo = new AsciiHistogram( stats_for_domain );
 708                 //System.out.println( histo.toStringBuffer( 40, '=', 60, 4 ).toString() );
 709             }
 710         }
 711         out.write( ForesterUtil.LINE_SEPARATOR );
 712         out.write( ForesterUtil.LINE_SEPARATOR );
 713         out.write( "OUTLIER SPECIES (Z 1.0)" );
 714         out.write( ForesterUtil.LINE_SEPARATOR );
 715         final DescriptiveStatistics stats_for_all_species = domain_lengths_table
 716                 .calculateMeanBasedStatisticsForAllSpecies();
 717         out.write( stats_for_all_species.asSummary() );
 718         out.write( ForesterUtil.LINE_SEPARATOR );
 719         final AsciiHistogram histo = new AsciiHistogram( stats_for_all_species );
 720         out.write( histo.toStringBuffer( 40, '=', 60, 4 ).toString() );
 721         out.write( ForesterUtil.LINE_SEPARATOR );
 722         final double population_sd = stats_for_all_species.sampleStandardDeviation();
 723         final double population_mean = stats_for_all_species.arithmeticMean();
 724         for( final Species species : domain_lengths_table.getSpecies() ) {
 725             final double x = domain_lengths_table.calculateMeanBasedStatisticsForSpecies( species ).arithmeticMean();
 726             final double z = ( x - population_mean ) / population_sd;
 727             out.write( species + "\t" + z );
 728             out.write( ForesterUtil.LINE_SEPARATOR );
 729         }
 730         out.write( ForesterUtil.LINE_SEPARATOR );
 731         for( final Species species : domain_lengths_table.getSpecies() ) {
 732             final DescriptiveStatistics stats_for_species = domain_lengths_table
 733                     .calculateMeanBasedStatisticsForSpecies( species );
 734             final double x = stats_for_species.arithmeticMean();
 735             final double z = ( x - population_mean ) / population_sd;
 736             if ( ( z <= -1.0 ) || ( z >= 1.0 ) ) {
 737                 out.write( species + "\t" + df.format( z ) + "\t" + stats_for_species.asSummary() );
 738                 out.write( ForesterUtil.LINE_SEPARATOR );
 739             }
 740         }
 741         out.close();
 742         //        final List<HistogramData> histogram_datas = new ArrayList<HistogramData>();
 743         //        for( int i = 0; i < number_of_genomes; ++i ) {
 744         //            final Species species = new BasicSpecies( input_file_properties[ i ][ 0 ] );
 745         //            histogram_datas
 746         //                    .add( new HistogramData( species.toString(), domain_lengths_table
 747         //                            .calculateMeanBasedStatisticsForSpecies( species )
 748         //                            .getDataAsDoubleArray(), 5, 600, null, 60 ) );
 749         //        }
 750         //        final HistogramsFrame hf = new HistogramsFrame( histogram_datas );
 751         //        hf.setVisible( true );
 752         System.gc();
 753     }
 754
 755     /**
 756      *
 757      * @param all_binary_domains_combination_lost_fitch
 758      * @param consider_directedness_and_adjacency_for_bin_combinations
 759      * @param all_binary_domains_combination_gained if null ignored, otherwise this is to list all binary domain combinations
 760      * which were gained under unweighted (Fitch) parsimony.
 761      */
 762     public static void executeParsimonyAnalysis( final long random_number_seed_for_fitch_parsimony,
 763                                                  final boolean radomize_fitch_parsimony,
 764                                                  final String outfile_name,
 765                                                  final DomainParsimonyCalculator domain_parsimony,
 766                                                  final Phylogeny phylogeny,
 767                                                  final Map<DomainId, List<GoId>> domain_id_to_go_ids_map,
 768                                                  final Map<GoId, GoTerm> go_id_to_term_map,
 769                                                  final GoNameSpace go_namespace_limit,
 770                                                  final String parameters_str,
 771                                                  final Map<DomainId, Set<String>>[] domain_id_to_secondary_features_maps,
 772                                                  final SortedSet<DomainId> positive_filter,
 773                                                  final boolean output_binary_domain_combinations_for_graphs,
 774                                                  final List<BinaryDomainCombination> all_binary_domains_combination_gained_fitch,
 775                                                  final List<BinaryDomainCombination> all_binary_domains_combination_lost_fitch,
 776                                                  final BinaryDomainCombination.DomainCombinationType dc_type,
 777                                                  final Map<String, DescriptiveStatistics> protein_length_stats_by_dc,
 778                                                  final Map<String, DescriptiveStatistics> domain_number_stats_by_dc,
 779                                                  final Map<String, DescriptiveStatistics> domain_length_stats_by_domain ) {
 780         final String sep = ForesterUtil.LINE_SEPARATOR + "###################" + ForesterUtil.LINE_SEPARATOR;
 781         final String date_time = ForesterUtil.getCurrentDateTime();
 782         final SortedSet<String> all_pfams_encountered = new TreeSet<String>();
 783         final SortedSet<String> all_pfams_gained_as_domains = new TreeSet<String>();
 784         final SortedSet<String> all_pfams_lost_as_domains = new TreeSet<String>();
 785         final SortedSet<String> all_pfams_gained_as_dom_combinations = new TreeSet<String>();
 786         final SortedSet<String> all_pfams_lost_as_dom_combinations = new TreeSet<String>();
 787         writeToNexus( outfile_name, domain_parsimony, phylogeny );
 788         // DOLLO DOMAINS
 789         // -------------
 790         Phylogeny local_phylogeny_l = phylogeny.copy();
 791         if ( ( positive_filter != null ) && ( positive_filter.size() > 0 ) ) {
 792             domain_parsimony.executeDolloParsimonyOnDomainPresence( positive_filter );
 793         }
 794         else {
 795             domain_parsimony.executeDolloParsimonyOnDomainPresence();
 796         }
 797         SurfacingUtil.writeMatrixToFile( domain_parsimony.getGainLossMatrix(), outfile_name
 798                 + surfacing.PARSIMONY_OUTPUT_GL_SUFFIX_DOLLO_DOMAINS, Format.FORESTER );
 799         SurfacingUtil.writeMatrixToFile( domain_parsimony.getGainLossCountsMatrix(), outfile_name
 800                 + surfacing.PARSIMONY_OUTPUT_GL_COUNTS_SUFFIX_DOLLO_DOMAINS, Format.FORESTER );
 801         SurfacingUtil.writeBinaryStatesMatrixAsListToFile( domain_parsimony.getGainLossMatrix(),
 802                                                            CharacterStateMatrix.GainLossStates.GAIN,
 803                                                            outfile_name + surfacing.PARSIMONY_OUTPUT_DOLLO_GAINS_D,
 804                                                            sep,
 805                                                            ForesterUtil.LINE_SEPARATOR,
 806                                                            null );
 807         SurfacingUtil.writeBinaryStatesMatrixAsListToFile( domain_parsimony.getGainLossMatrix(),
 808                                                            CharacterStateMatrix.GainLossStates.LOSS,
 809                                                            outfile_name + surfacing.PARSIMONY_OUTPUT_DOLLO_LOSSES_D,
 810                                                            sep,
 811                                                            ForesterUtil.LINE_SEPARATOR,
 812                                                            null );
 813         SurfacingUtil.writeBinaryStatesMatrixAsListToFile( domain_parsimony.getGainLossMatrix(), null, outfile_name
 814                 + surfacing.PARSIMONY_OUTPUT_DOLLO_PRESENT_D, sep, ForesterUtil.LINE_SEPARATOR, null );
 815         //HTML:
 816         writeBinaryStatesMatrixToList( domain_id_to_go_ids_map,
 817                                        go_id_to_term_map,
 818                                        go_namespace_limit,
 819                                        false,
 820                                        domain_parsimony.getGainLossMatrix(),
 821                                        CharacterStateMatrix.GainLossStates.GAIN,
 822                                        outfile_name + surfacing.PARSIMONY_OUTPUT_DOLLO_GAINS_HTML_D,
 823                                        sep,
 824                                        ForesterUtil.LINE_SEPARATOR,
 825                                        "Dollo Parsimony | Gains | Domains",
 826                                        "+",
 827                                        domain_id_to_secondary_features_maps,
 828                                        all_pfams_encountered,
 829                                        all_pfams_gained_as_domains,
 830                                        "_dollo_gains_d" );
 831         writeBinaryStatesMatrixToList( domain_id_to_go_ids_map,
 832                                        go_id_to_term_map,
 833                                        go_namespace_limit,
 834                                        false,
 835                                        domain_parsimony.getGainLossMatrix(),
 836                                        CharacterStateMatrix.GainLossStates.LOSS,
 837                                        outfile_name + surfacing.PARSIMONY_OUTPUT_DOLLO_LOSSES_HTML_D,
 838                                        sep,
 839                                        ForesterUtil.LINE_SEPARATOR,
 840                                        "Dollo Parsimony | Losses | Domains",
 841                                        "-",
 842                                        domain_id_to_secondary_features_maps,
 843                                        all_pfams_encountered,
 844                                        all_pfams_lost_as_domains,
 845                                        "_dollo_losses_d" );
 846         writeBinaryStatesMatrixToList( domain_id_to_go_ids_map,
 847                                        go_id_to_term_map,
 848                                        go_namespace_limit,
 849                                        false,
 850                                        domain_parsimony.getGainLossMatrix(),
 851                                        null,
 852                                        outfile_name + surfacing.PARSIMONY_OUTPUT_DOLLO_PRESENT_HTML_D,
 853                                        sep,
 854                                        ForesterUtil.LINE_SEPARATOR,
 855                                        "Dollo Parsimony | Present | Domains",
 856                                        "",
 857                                        domain_id_to_secondary_features_maps,
 858                                        all_pfams_encountered,
 859                                        null,
 860                                        "_dollo_present_d" );
 861         preparePhylogeny( local_phylogeny_l,
 862                           domain_parsimony,
 863                           date_time,
 864                           "Dollo parsimony on domain presence/absence",
 865                           "dollo_on_domains_" + outfile_name,
 866                           parameters_str );
 867         SurfacingUtil.writePhylogenyToFile( local_phylogeny_l, outfile_name
 868                 + surfacing.DOMAINS_PARSIMONY_TREE_OUTPUT_SUFFIX_DOLLO );
 869         try {
 870             writeAllDomainsChangedOnAllSubtrees( local_phylogeny_l, true, outfile_name, "_dollo_all_gains_d" );
 871             writeAllDomainsChangedOnAllSubtrees( local_phylogeny_l, false, outfile_name, "_dollo_all_losses_d" );
 872         }
 873         catch ( final IOException e ) {
 874             e.printStackTrace();
 875             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getLocalizedMessage() );
 876         }
 877         if ( domain_parsimony.calculateNumberOfBinaryDomainCombination() > 0 ) {
 878             // FITCH DOMAIN COMBINATIONS
 879             // -------------------------
 880             local_phylogeny_l = phylogeny.copy();
 881             String randomization = "no";
 882             if ( radomize_fitch_parsimony ) {
 883                 domain_parsimony.executeFitchParsimonyOnBinaryDomainCombintion( random_number_seed_for_fitch_parsimony );
 884                 randomization = "yes, seed = " + random_number_seed_for_fitch_parsimony;
 885             }
 886             else {
 887                 domain_parsimony.executeFitchParsimonyOnBinaryDomainCombintion( USE_LAST );
 888             }
 889             SurfacingUtil.writeMatrixToFile( domain_parsimony.getGainLossMatrix(), outfile_name
 890                     + surfacing.PARSIMONY_OUTPUT_GL_SUFFIX_FITCH_BINARY_COMBINATIONS, Format.FORESTER );
 891             SurfacingUtil.writeMatrixToFile( domain_parsimony.getGainLossCountsMatrix(), outfile_name
 892                     + surfacing.PARSIMONY_OUTPUT_GL_COUNTS_SUFFIX_FITCH_BINARY_COMBINATIONS, Format.FORESTER );
 893             SurfacingUtil
 894                     .writeBinaryStatesMatrixAsListToFile( domain_parsimony.getGainLossMatrix(),
 895                                                           CharacterStateMatrix.GainLossStates.GAIN,
 896                                                           outfile_name + surfacing.PARSIMONY_OUTPUT_FITCH_GAINS_BC,
 897                                                           sep,
 898                                                           ForesterUtil.LINE_SEPARATOR,
 899                                                           null );
 900             SurfacingUtil.writeBinaryStatesMatrixAsListToFile( domain_parsimony.getGainLossMatrix(),
 901                                                                CharacterStateMatrix.GainLossStates.LOSS,
 902                                                                outfile_name
 903                                                                        + surfacing.PARSIMONY_OUTPUT_FITCH_LOSSES_BC,
 904                                                                sep,
 905                                                                ForesterUtil.LINE_SEPARATOR,
 906                                                                null );
 907             SurfacingUtil.writeBinaryStatesMatrixAsListToFile( domain_parsimony.getGainLossMatrix(), null, outfile_name
 908                     + surfacing.PARSIMONY_OUTPUT_FITCH_PRESENT_BC, sep, ForesterUtil.LINE_SEPARATOR, null );
 909             if ( all_binary_domains_combination_gained_fitch != null ) {
 910                 collectChangedDomainCombinationsFromBinaryStatesMatrixAsListToFile( domain_parsimony.getGainLossMatrix(),
 911                                                                                     dc_type,
 912                                                                                     all_binary_domains_combination_gained_fitch,
 913                                                                                     true );
 914             }
 915             if ( all_binary_domains_combination_lost_fitch != null ) {
 916                 collectChangedDomainCombinationsFromBinaryStatesMatrixAsListToFile( domain_parsimony.getGainLossMatrix(),
 917                                                                                     dc_type,
 918                                                                                     all_binary_domains_combination_lost_fitch,
 919                                                                                     false );
 920             }
 921             if ( output_binary_domain_combinations_for_graphs ) {
 922                 SurfacingUtil
 923                         .writeBinaryStatesMatrixAsListToFileForBinaryCombinationsForGraphAnalysis( domain_parsimony
 924                                                                                                            .getGainLossMatrix(),
 925                                                                                                    null,
 926                                                                                                    outfile_name
 927                                                                                                            + surfacing.PARSIMONY_OUTPUT_FITCH_PRESENT_BC_OUTPUTFILE_SUFFIX_FOR_GRAPH_ANALYSIS,
 928                                                                                                    sep,
 929                                                                                                    ForesterUtil.LINE_SEPARATOR,
 930                                                                                                    BinaryDomainCombination.OutputFormat.DOT );
 931             }
 932             // HTML:
 933             writeBinaryStatesMatrixToList( domain_id_to_go_ids_map,
 934                                            go_id_to_term_map,
 935                                            go_namespace_limit,
 936                                            true,
 937                                            domain_parsimony.getGainLossMatrix(),
 938                                            CharacterStateMatrix.GainLossStates.GAIN,
 939                                            outfile_name + surfacing.PARSIMONY_OUTPUT_FITCH_GAINS_HTML_BC,
 940                                            sep,
 941                                            ForesterUtil.LINE_SEPARATOR,
 942                                            "Fitch Parsimony | Gains | Domain Combinations",
 943                                            "+",
 944                                            null,
 945                                            all_pfams_encountered,
 946                                            all_pfams_gained_as_dom_combinations,
 947                                            "_fitch_gains_dc" );
 948             writeBinaryStatesMatrixToList( domain_id_to_go_ids_map,
 949                                            go_id_to_term_map,
 950                                            go_namespace_limit,
 951                                            true,
 952                                            domain_parsimony.getGainLossMatrix(),
 953                                            CharacterStateMatrix.GainLossStates.LOSS,
 954                                            outfile_name + surfacing.PARSIMONY_OUTPUT_FITCH_LOSSES_HTML_BC,
 955                                            sep,
 956                                            ForesterUtil.LINE_SEPARATOR,
 957                                            "Fitch Parsimony | Losses | Domain Combinations",
 958                                            "-",
 959                                            null,
 960                                            all_pfams_encountered,
 961                                            all_pfams_lost_as_dom_combinations,
 962                                            "_fitch_losses_dc" );
 963             writeBinaryStatesMatrixToList( domain_id_to_go_ids_map,
 964                                            go_id_to_term_map,
 965                                            go_namespace_limit,
 966                                            true,
 967                                            domain_parsimony.getGainLossMatrix(),
 968                                            null,
 969                                            outfile_name + surfacing.PARSIMONY_OUTPUT_FITCH_PRESENT_HTML_BC,
 970                                            sep,
 971                                            ForesterUtil.LINE_SEPARATOR,
 972                                            "Fitch Parsimony | Present | Domain Combinations",
 973                                            "",
 974                                            null,
 975                                            all_pfams_encountered,
 976                                            null,
 977                                            "_fitch_present_dc" );
 978             writeAllEncounteredPfamsToFile( domain_id_to_go_ids_map,
 979                                             go_id_to_term_map,
 980                                             outfile_name,
 981                                             all_pfams_encountered );
 982             writePfamsToFile( outfile_name + surfacing.ALL_PFAMS_GAINED_AS_DOMAINS_SUFFIX, all_pfams_gained_as_domains );
 983             writePfamsToFile( outfile_name + surfacing.ALL_PFAMS_LOST_AS_DOMAINS_SUFFIX, all_pfams_lost_as_domains );
 984             writePfamsToFile( outfile_name + surfacing.ALL_PFAMS_GAINED_AS_DC_SUFFIX,
 985                               all_pfams_gained_as_dom_combinations );
 986             writePfamsToFile( outfile_name + surfacing.ALL_PFAMS_LOST_AS_DC_SUFFIX, all_pfams_lost_as_dom_combinations );
 987             preparePhylogeny( local_phylogeny_l,
 988                               domain_parsimony,
 989                               date_time,
 990                               "Fitch parsimony on binary domain combination presence/absence randomization: "
 991                                       + randomization,
 992                               "fitch_on_binary_domain_combinations_" + outfile_name,
 993                               parameters_str );
 994             SurfacingUtil.writePhylogenyToFile( local_phylogeny_l, outfile_name
 995                     + surfacing.BINARY_DOMAIN_COMBINATIONS_PARSIMONY_TREE_OUTPUT_SUFFIX_FITCH );
 996             calculateIndependentDomainCombinationGains( local_phylogeny_l,
 997                                                         outfile_name
 998                                                                 + surfacing.INDEPENDENT_DC_GAINS_FITCH_PARS_COUNTS_OUTPUT_SUFFIX,
 999                                                         outfile_name
1000                                                                 + surfacing.INDEPENDENT_DC_GAINS_FITCH_PARS_DC_OUTPUT_SUFFIX,
1001                                                         outfile_name
1002                                                                 + surfacing.INDEPENDENT_DC_GAINS_FITCH_PARS_DC_FOR_GO_MAPPING_OUTPUT_SUFFIX,
1003                                                         outfile_name
1004                                                                 + surfacing.INDEPENDENT_DC_GAINS_FITCH_PARS_DC_FOR_GO_MAPPING_OUTPUT_UNIQUE_SUFFIX,
1005                                                         outfile_name + "_indep_dc_gains_fitch_lca_ranks.txt",
1006                                                         outfile_name + "_indep_dc_gains_fitch_lca_taxonomies.txt",
1007                                                         outfile_name + "_indep_dc_gains_fitch_protein_statistics.txt",
1008                                                         protein_length_stats_by_dc,
1009                                                         domain_number_stats_by_dc,
1010                                                         domain_length_stats_by_domain );
1011         }
1012     }
1013
1014     public static void executeParsimonyAnalysisForSecondaryFeatures( final String outfile_name,
1015                                                                      final DomainParsimonyCalculator secondary_features_parsimony,
1016                                                                      final Phylogeny phylogeny,
1017                                                                      final String parameters_str,
1018                                                                      final Map<Species, MappingResults> mapping_results_map ) {
1019         final String sep = ForesterUtil.LINE_SEPARATOR + "###################" + ForesterUtil.LINE_SEPARATOR;
1020         final String date_time = ForesterUtil.getCurrentDateTime();
1021         System.out.println();
1022         writeToNexus( outfile_name + surfacing.NEXUS_SECONDARY_FEATURES,
1023                       secondary_features_parsimony.createMatrixOfSecondaryFeaturePresenceOrAbsence( null ),
1024                       phylogeny );
1025         Phylogeny local_phylogeny_copy = phylogeny.copy();
1026         secondary_features_parsimony.executeDolloParsimonyOnSecondaryFeatures( mapping_results_map );
1027         SurfacingUtil.writeMatrixToFile( secondary_features_parsimony.getGainLossMatrix(), outfile_name
1028                 + surfacing.PARSIMONY_OUTPUT_GL_SUFFIX_DOLLO_SECONDARY_FEATURES, Format.FORESTER );
1029         SurfacingUtil.writeMatrixToFile( secondary_features_parsimony.getGainLossCountsMatrix(), outfile_name
1030                 + surfacing.PARSIMONY_OUTPUT_GL_COUNTS_SUFFIX_DOLLO_SECONDARY_FEATURES, Format.FORESTER );
1031         SurfacingUtil
1032                 .writeBinaryStatesMatrixAsListToFile( secondary_features_parsimony.getGainLossMatrix(),
1033                                                       CharacterStateMatrix.GainLossStates.GAIN,
1034                                                       outfile_name
1035                                                               + surfacing.PARSIMONY_OUTPUT_DOLLO_GAINS_SECONDARY_FEATURES,
1036                                                       sep,
1037                                                       ForesterUtil.LINE_SEPARATOR,
1038                                                       null );
1039         SurfacingUtil
1040                 .writeBinaryStatesMatrixAsListToFile( secondary_features_parsimony.getGainLossMatrix(),
1041                                                       CharacterStateMatrix.GainLossStates.LOSS,
1042                                                       outfile_name
1043                                                               + surfacing.PARSIMONY_OUTPUT_DOLLO_LOSSES_SECONDARY_FEATURES,
1044                                                       sep,
1045                                                       ForesterUtil.LINE_SEPARATOR,
1046                                                       null );
1047         SurfacingUtil
1048                 .writeBinaryStatesMatrixAsListToFile( secondary_features_parsimony.getGainLossMatrix(),
1049                                                       null,
1050                                                       outfile_name
1051                                                               + surfacing.PARSIMONY_OUTPUT_DOLLO_PRESENT_SECONDARY_FEATURES,
1052                                                       sep,
1053                                                       ForesterUtil.LINE_SEPARATOR,
1054                                                       null );
1055         preparePhylogeny( local_phylogeny_copy,
1056                           secondary_features_parsimony,
1057                           date_time,
1058                           "Dollo parsimony on secondary feature presence/absence",
1059                           "dollo_on_secondary_features_" + outfile_name,
1060                           parameters_str );
1061         SurfacingUtil.writePhylogenyToFile( local_phylogeny_copy, outfile_name
1062                 + surfacing.SECONDARY_FEATURES_PARSIMONY_TREE_OUTPUT_SUFFIX_DOLLO );
1063         // FITCH DOMAIN COMBINATIONS
1064         // -------------------------
1065         local_phylogeny_copy = phylogeny.copy();
1066         final String randomization = "no";
1067         secondary_features_parsimony.executeFitchParsimonyOnBinaryDomainCombintionOnSecondaryFeatures( USE_LAST );
1068         preparePhylogeny( local_phylogeny_copy,
1069                           secondary_features_parsimony,
1070                           date_time,
1071                           "Fitch parsimony on secondary binary domain combination presence/absence randomization: "
1072                                   + randomization,
1073                           "fitch_on_binary_domain_combinations_" + outfile_name,
1074                           parameters_str );
1075         SurfacingUtil.writePhylogenyToFile( local_phylogeny_copy, outfile_name
1076                 + surfacing.BINARY_DOMAIN_COMBINATIONS_PARSIMONY_TREE_OUTPUT_SUFFIX_FITCH_MAPPED );
1077         calculateIndependentDomainCombinationGains( local_phylogeny_copy, outfile_name
1078                 + surfacing.INDEPENDENT_DC_GAINS_FITCH_PARS_COUNTS_MAPPED_OUTPUT_SUFFIX, outfile_name
1079                 + surfacing.INDEPENDENT_DC_GAINS_FITCH_PARS_DC_MAPPED_OUTPUT_SUFFIX, outfile_name
1080                 + surfacing.INDEPENDENT_DC_GAINS_FITCH_PARS_DC_FOR_GO_MAPPING_MAPPED_OUTPUT_SUFFIX, outfile_name
1081                 + surfacing.INDEPENDENT_DC_GAINS_FITCH_PARS_DC_FOR_GO_MAPPING_MAPPED_OUTPUT_UNIQUE_SUFFIX, outfile_name
1082                 + "_MAPPED_indep_dc_gains_fitch_lca_ranks.txt", outfile_name
1083                 + "_MAPPED_indep_dc_gains_fitch_lca_taxonomies.txt", null, null, null, null );
1084     }
1085
1086     public static void doit( final List<Protein> proteins,
1087                              final List<DomainId> query_domain_ids_nc_order,
1088                              final Writer out,
1089                              final String separator,
1090                              final String limit_to_species,
1091                              final Map<String, List<Integer>> average_protein_lengths_by_dc ) throws IOException {
1092         for( final Protein protein : proteins ) {
1093             if ( ForesterUtil.isEmpty( limit_to_species )
1094                     || protein.getSpecies().getSpeciesId().equalsIgnoreCase( limit_to_species ) ) {
1095                 if ( protein.contains( query_domain_ids_nc_order, true ) ) {
1096                     out.write( protein.getSpecies().getSpeciesId() );
1097                     out.write( separator );
1098                     out.write( protein.getProteinId().getId() );
1099                     out.write( separator );
1100                     out.write( "[" );
1101                     final Set<DomainId> visited_domain_ids = new HashSet<DomainId>();
1102                     boolean first = true;
1103                     for( final Domain domain : protein.getProteinDomains() ) {
1104                         if ( !visited_domain_ids.contains( domain.getDomainId() ) ) {
1105                             visited_domain_ids.add( domain.getDomainId() );
1106                             if ( first ) {
1107                                 first = false;
1108                             }
1109                             else {
1110                                 out.write( " " );
1111                             }
1112                             out.write( domain.getDomainId().getId() );
1113                             out.write( " {" );
1114                             out.write( "" + domain.getTotalCount() );
1115                             out.write( "}" );
1116                         }
1117                     }
1118                     out.write( "]" );
1119                     out.write( separator );
1120                     if ( !( ForesterUtil.isEmpty( protein.getDescription() ) || protein.getDescription()
1121                             .equals( SurfacingConstants.NONE ) ) ) {
1122                         out.write( protein.getDescription() );
1123                     }
1124                     out.write( separator );
1125                     if ( !( ForesterUtil.isEmpty( protein.getAccession() ) || protein.getAccession()
1126                             .equals( SurfacingConstants.NONE ) ) ) {
1127                         out.write( protein.getAccession() );
1128                     }
1129                     out.write( SurfacingConstants.NL );
1130                 }
1131             }
1132         }
1133         out.flush();
1134     }
1135
1136     public static void extractProteinNames( final List<Protein> proteins,
1137                                             final List<DomainId> query_domain_ids_nc_order,
1138                                             final Writer out,
1139                                             final String separator,
1140                                             final String limit_to_species ) throws IOException {
1141         for( final Protein protein : proteins ) {
1142             if ( ForesterUtil.isEmpty( limit_to_species )
1143                     || protein.getSpecies().getSpeciesId().equalsIgnoreCase( limit_to_species ) ) {
1144                 if ( protein.contains( query_domain_ids_nc_order, true ) ) {
1145                     out.write( protein.getSpecies().getSpeciesId() );
1146                     out.write( separator );
1147                     out.write( protein.getProteinId().getId() );
1148                     out.write( separator );
1149                     out.write( "[" );
1150                     final Set<DomainId> visited_domain_ids = new HashSet<DomainId>();
1151                     boolean first = true;
1152                     for( final Domain domain : protein.getProteinDomains() ) {
1153                         if ( !visited_domain_ids.contains( domain.getDomainId() ) ) {
1154                             visited_domain_ids.add( domain.getDomainId() );
1155                             if ( first ) {
1156                                 first = false;
1157                             }
1158                             else {
1159                                 out.write( " " );
1160                             }
1161                             out.write( domain.getDomainId().getId() );
1162                             out.write( " {" );
1163                             out.write( "" + domain.getTotalCount() );
1164                             out.write( "}" );
1165                         }
1166                     }
1167                     out.write( "]" );
1168                     out.write( separator );
1169                     if ( !( ForesterUtil.isEmpty( protein.getDescription() ) || protein.getDescription()
1170                             .equals( SurfacingConstants.NONE ) ) ) {
1171                         out.write( protein.getDescription() );
1172                     }
1173                     out.write( separator );
1174                     if ( !( ForesterUtil.isEmpty( protein.getAccession() ) || protein.getAccession()
1175                             .equals( SurfacingConstants.NONE ) ) ) {
1176                         out.write( protein.getAccession() );
1177                     }
1178                     out.write( SurfacingConstants.NL );
1179                 }
1180             }
1181         }
1182         out.flush();
1183     }
1184
1185     public static void extractProteinNames( final SortedMap<Species, List<Protein>> protein_lists_per_species,
1186                                             final DomainId domain_id,
1187                                             final Writer out,
1188                                             final String separator,
1189                                             final String limit_to_species,
1190                                             final double domain_e_cutoff ) throws IOException {
1191         for( final Species species : protein_lists_per_species.keySet() ) {
1192             for( final Protein protein : protein_lists_per_species.get( species ) ) {
1193                 if ( ForesterUtil.isEmpty( limit_to_species )
1194                         || protein.getSpecies().getSpeciesId().equalsIgnoreCase( limit_to_species ) ) {
1195                     final List<Domain> domains = protein.getProteinDomains( domain_id );
1196                     if ( domains.size() > 0 ) {
1197                         out.write( protein.getSpecies().getSpeciesId() );
1198                         out.write( separator );
1199                         out.write( protein.getProteinId().getId() );
1200                         out.write( separator );
1201                         out.write( domain_id.toString() );
1202                         out.write( separator );
1203                         for( final Domain domain : domains ) {
1204                             if ( domain_e_cutoff < 0 || domain.getPerDomainEvalue() <= domain_e_cutoff ) {
1205                                  out.write( "/" );
1206                                  out.write( domain.getFrom() + "-" + domain.getTo() );
1207                             }
1208                         }
1209                         out.write( "/" );
1210                         out.write( separator );
1211
1212                         final List<Domain> domain_list = new ArrayList<Domain>();
1213
1214                         for( final Domain domain : protein.getProteinDomains() ) {
1215                             if ( domain_e_cutoff < 0 || domain.getPerDomainEvalue() <= domain_e_cutoff ) {
1216                                 domain_list.add( domain );
1217                             }
1218                         }
1219
1220                         Domain domain_ary[] = new Domain[ domain_list.size() ];
1221
1222                         for( int i = 0; i < domain_list.size(); ++i ) {
1223                             domain_ary[ i ] = domain_list.get( i );
1224                         }
1225
1226                         Arrays.sort( domain_ary, new DomainComparator( true ) );
1227
1228                         out.write( "{" );
1229                         boolean first = true;
1230
1231                         for( final Domain domain : domain_ary ) {
1232                             if ( first ) {
1233                                 first = false;
1234                             }
1235                             else {
1236                                 out.write( "," );
1237                             }
1238                             out.write( domain.getDomainId().toString() );
1239                             out.write( ":" + domain.getFrom() +  "-" + domain.getTo() );
1240                             out.write( ":" + domain.getPerDomainEvalue() );
1241                         }
1242                         out.write( "}" );
1243                         if ( !( ForesterUtil.isEmpty( protein.getDescription() ) || protein.getDescription()
1244                                 .equals( SurfacingConstants.NONE ) ) ) {
1245                             out.write( protein.getDescription() );
1246                         }
1247                         out.write( separator );
1248                         if ( !( ForesterUtil.isEmpty( protein.getAccession() ) || protein.getAccession()
1249                                 .equals( SurfacingConstants.NONE ) ) ) {
1250                             out.write( protein.getAccession() );
1251                         }
1252                         out.write( SurfacingConstants.NL );
1253                     }
1254                 }
1255             }
1256         }
1257         out.flush();
1258     }
1259
1260     public static SortedSet<DomainId> getAllDomainIds( final List<GenomeWideCombinableDomains> gwcd_list ) {
1261         final SortedSet<DomainId> all_domains_ids = new TreeSet<DomainId>();
1262         for( final GenomeWideCombinableDomains gwcd : gwcd_list ) {
1263             final Set<DomainId> all_domains = gwcd.getAllDomainIds();
1264             //    for( final Domain domain : all_domains ) {
1265             all_domains_ids.addAll( all_domains );
1266             //    }
1267         }
1268         return all_domains_ids;
1269     }
1270
1271     public static SortedMap<String, Integer> getDomainCounts( final List<Protein> protein_domain_collections ) {
1272         final SortedMap<String, Integer> map = new TreeMap<String, Integer>();
1273         for( final Protein protein_domain_collection : protein_domain_collections ) {
1274             for( final Object name : protein_domain_collection.getProteinDomains() ) {
1275                 final BasicDomain protein_domain = ( BasicDomain ) name;
1276                 final String id = protein_domain.getDomainId().getId();
1277                 if ( map.containsKey( id ) ) {
1278                     map.put( id, map.get( id ) + 1 );
1279                 }
1280                 else {
1281                     map.put( id, 1 );
1282                 }
1283             }
1284         }
1285         return map;
1286     }
1287
1288     public static int getNumberOfNodesLackingName( final Phylogeny p, final StringBuilder names ) {
1289         final PhylogenyNodeIterator it = p.iteratorPostorder();
1290         int c = 0;
1291         while ( it.hasNext() ) {
1292             final PhylogenyNode n = it.next();
1293             if ( ForesterUtil.isEmpty( n.getName() )
1294                     && ( !n.getNodeData().isHasTaxonomy() || ForesterUtil.isEmpty( n.getNodeData().getTaxonomy()
1295                             .getScientificName() ) )
1296                     && ( !n.getNodeData().isHasTaxonomy() || ForesterUtil.isEmpty( n.getNodeData().getTaxonomy()
1297                             .getCommonName() ) ) ) {
1298                 if ( n.getParent() != null ) {
1299                     names.append( " " );
1300                     names.append( n.getParent().getName() );
1301                 }
1302                 final List l = n.getAllExternalDescendants();
1303                 for( final Object object : l ) {
1304                     System.out.println( l.toString() );
1305                 }
1306                 ++c;
1307             }
1308         }
1309         return c;
1310     }
1311
1312     /**
1313      * Returns true is Domain domain falls in an uninterrupted stretch of
1314      * covered positions.
1315      *
1316      * @param domain
1317      * @param covered_positions
1318      * @return
1319      */
1320     public static boolean isEngulfed( final Domain domain, final List<Boolean> covered_positions ) {
1321         for( int i = domain.getFrom(); i <= domain.getTo(); ++i ) {
1322             if ( ( i >= covered_positions.size() ) || ( covered_positions.get( i ) != true ) ) {
1323                 return false;
1324             }
1325         }
1326         return true;
1327     }
1328
1329     public static void preparePhylogeny( final Phylogeny p,
1330                                          final DomainParsimonyCalculator domain_parsimony,
1331                                          final String date_time,
1332                                          final String method,
1333                                          final String name,
1334                                          final String parameters_str ) {
1335         domain_parsimony.decoratePhylogenyWithDomains( p );
1336         final StringBuilder desc = new StringBuilder();
1337         desc.append( "[Method: " + method + "] [Date: " + date_time + "] " );
1338         desc.append( "[Cost: " + domain_parsimony.getCost() + "] " );
1339         desc.append( "[Gains: " + domain_parsimony.getTotalGains() + "] " );
1340         desc.append( "[Losses: " + domain_parsimony.getTotalLosses() + "] " );
1341         desc.append( "[Unchanged: " + domain_parsimony.getTotalUnchanged() + "] " );
1342         desc.append( "[Parameters: " + parameters_str + "]" );
1343         p.setName( name );
1344         p.setDescription( desc.toString() );
1345         p.setConfidence( new Confidence( domain_parsimony.getCost(), "parsimony" ) );
1346         p.setRerootable( false );
1347         p.setRooted( true );
1348     }
1349
1350     /*
1351      * species | protein id | n-terminal domain | c-terminal domain | n-terminal domain per domain E-value | c-terminal domain per domain E-value
1352      *
1353      *
1354      */
1355     static public StringBuffer proteinToDomainCombinations( final Protein protein,
1356                                                             final String protein_id,
1357                                                             final String separator ) {
1358         final StringBuffer sb = new StringBuffer();
1359         if ( protein.getSpecies() == null ) {
1360             throw new IllegalArgumentException( "species must not be null" );
1361         }
1362         if ( ForesterUtil.isEmpty( protein.getSpecies().getSpeciesId() ) ) {
1363             throw new IllegalArgumentException( "species id must not be empty" );
1364         }
1365         final List<Domain> domains = protein.getProteinDomains();
1366         if ( domains.size() > 1 ) {
1367             final Map<String, Integer> counts = new HashMap<String, Integer>();
1368             for( final Domain domain : domains ) {
1369                 final String id = domain.getDomainId().getId();
1370                 if ( counts.containsKey( id ) ) {
1371                     counts.put( id, counts.get( id ) + 1 );
1372                 }
1373                 else {
1374                     counts.put( id, 1 );
1375                 }
1376             }
1377             final Set<String> dcs = new HashSet<String>();
1378             for( int i = 1; i < domains.size(); ++i ) {
1379                 for( int j = 0; j < i; ++j ) {
1380                     Domain domain_n = domains.get( i );
1381                     Domain domain_c = domains.get( j );
1382                     if ( domain_n.getFrom() > domain_c.getFrom() ) {
1383                         domain_n = domains.get( j );
1384                         domain_c = domains.get( i );
1385                     }
1386                     final String dc = domain_n.getDomainId().getId() + domain_c.getDomainId().getId();
1387                     if ( !dcs.contains( dc ) ) {
1388                         dcs.add( dc );
1389                         sb.append( protein.getSpecies() );
1390                         sb.append( separator );
1391                         sb.append( protein_id );
1392                         sb.append( separator );
1393                         sb.append( domain_n.getDomainId().getId() );
1394                         sb.append( separator );
1395                         sb.append( domain_c.getDomainId().getId() );
1396                         sb.append( separator );
1397                         sb.append( domain_n.getPerDomainEvalue() );
1398                         sb.append( separator );
1399                         sb.append( domain_c.getPerDomainEvalue() );
1400                         sb.append( separator );
1401                         sb.append( counts.get( domain_n.getDomainId().getId() ) );
1402                         sb.append( separator );
1403                         sb.append( counts.get( domain_c.getDomainId().getId() ) );
1404                         sb.append( ForesterUtil.LINE_SEPARATOR );
1405                     }
1406                 }
1407             }
1408         }
1409         else if ( domains.size() == 1 ) {
1410             sb.append( protein.getSpecies() );
1411             sb.append( separator );
1412             sb.append( protein_id );
1413             sb.append( separator );
1414             sb.append( domains.get( 0 ).getDomainId().getId() );
1415             sb.append( separator );
1416             sb.append( separator );
1417             sb.append( domains.get( 0 ).getPerDomainEvalue() );
1418             sb.append( separator );
1419             sb.append( separator );
1420             sb.append( 1 );
1421             sb.append( separator );
1422             sb.append( ForesterUtil.LINE_SEPARATOR );
1423         }
1424         else {
1425             sb.append( protein.getSpecies() );
1426             sb.append( separator );
1427             sb.append( protein_id );
1428             sb.append( separator );
1429             sb.append( separator );
1430             sb.append( separator );
1431             sb.append( separator );
1432             sb.append( separator );
1433             sb.append( separator );
1434             sb.append( ForesterUtil.LINE_SEPARATOR );
1435         }
1436         return sb;
1437     }
1438
1439     /**
1440      *
1441      * Example regarding engulfment: ------------0.1 ----------0.2 --0.3 =>
1442      * domain with 0.3 is ignored
1443      *
1444      * -----------0.1 ----------0.2 --0.3 => domain with 0.3 is ignored
1445      *
1446      *
1447      * ------------0.1 ----------0.3 --0.2 => domains with 0.3 and 0.2 are _not_
1448      * ignored
1449      *
1450      * @param max_allowed_overlap
1451      *            maximal allowed overlap (inclusive) to be still considered not
1452      *            overlapping (zero or negative value to allow any overlap)
1453      * @param remove_engulfed_domains
1454      *            to remove domains which are completely engulfed by coverage of
1455      *            domains with better support
1456      * @param protein
1457      * @return
1458      */
1459     public static Protein removeOverlappingDomains( final int max_allowed_overlap,
1460                                                     final boolean remove_engulfed_domains,
1461                                                     final Protein protein ) {
1462         final Protein pruned_protein = new BasicProtein( protein.getProteinId().getId(), protein.getSpecies()
1463                 .getSpeciesId(), protein.getLength() );
1464         final List<Domain> sorted = SurfacingUtil.sortDomainsWithAscendingConfidenceValues( protein );
1465         final List<Boolean> covered_positions = new ArrayList<Boolean>();
1466         for( final Domain domain : sorted ) {
1467             if ( ( ( max_allowed_overlap < 0 ) || ( SurfacingUtil.calculateOverlap( domain, covered_positions ) <= max_allowed_overlap ) )
1468                     && ( !remove_engulfed_domains || !isEngulfed( domain, covered_positions ) ) ) {
1469                 final int covered_positions_size = covered_positions.size();
1470                 for( int i = covered_positions_size; i < domain.getFrom(); ++i ) {
1471                     covered_positions.add( false );
1472                 }
1473                 final int new_covered_positions_size = covered_positions.size();
1474                 for( int i = domain.getFrom(); i <= domain.getTo(); ++i ) {
1475                     if ( i < new_covered_positions_size ) {
1476                         covered_positions.set( i, true );
1477                     }
1478                     else {
1479                         covered_positions.add( true );
1480                     }
1481                 }
1482                 pruned_protein.addProteinDomain( domain );
1483             }
1484         }
1485         return pruned_protein;
1486     }
1487
1488     public static List<Domain> sortDomainsWithAscendingConfidenceValues( final Protein protein ) {
1489         final List<Domain> domains = new ArrayList<Domain>();
1490         for( final Domain d : protein.getProteinDomains() ) {
1491             domains.add( d );
1492         }
1493         Collections.sort( domains, SurfacingUtil.ASCENDING_CONFIDENCE_VALUE_ORDER );
1494         return domains;
1495     }
1496
1497     private static List<String> splitDomainCombination( final String dc ) {
1498         final String[] s = dc.split( "=" );
1499         if ( s.length != 2 ) {
1500             ForesterUtil.printErrorMessage( surfacing.PRG_NAME, "Stringyfied domain combination has illegal format: "
1501                     + dc );
1502             System.exit( -1 );
1503         }
1504         final List<String> l = new ArrayList<String>( 2 );
1505         l.add( s[ 0 ] );
1506         l.add( s[ 1 ] );
1507         return l;
1508     }
1509
1510     public static void writeAllDomainsChangedOnAllSubtrees( final Phylogeny p,
1511                                                             final boolean get_gains,
1512                                                             final String outdir,
1513                                                             final String suffix_for_filename ) throws IOException {
1514         CharacterStateMatrix.GainLossStates state = CharacterStateMatrix.GainLossStates.GAIN;
1515         if ( !get_gains ) {
1516             state = CharacterStateMatrix.GainLossStates.LOSS;
1517         }
1518         final File base_dir = createBaseDirForPerNodeDomainFiles( surfacing.BASE_DIRECTORY_PER_SUBTREE_DOMAIN_GAIN_LOSS_FILES,
1519                                                                   false,
1520                                                                   state,
1521                                                                   outdir );
1522         for( final PhylogenyNodeIterator it = p.iteratorPostorder(); it.hasNext(); ) {
1523             final PhylogenyNode node = it.next();
1524             if ( !node.isExternal() ) {
1525                 final SortedSet<String> domains = collectAllDomainsChangedOnSubtree( node, get_gains );
1526                 if ( domains.size() > 0 ) {
1527                     final Writer writer = ForesterUtil.createBufferedWriter( base_dir + ForesterUtil.FILE_SEPARATOR
1528                             + node.getName() + suffix_for_filename );
1529                     for( final String domain : domains ) {
1530                         writer.write( domain );
1531                         writer.write( ForesterUtil.LINE_SEPARATOR );
1532                     }
1533                     writer.close();
1534                 }
1535             }
1536         }
1537     }
1538
1539     private static void writeAllEncounteredPfamsToFile( final Map<DomainId, List<GoId>> domain_id_to_go_ids_map,
1540                                                         final Map<GoId, GoTerm> go_id_to_term_map,
1541                                                         final String outfile_name,
1542                                                         final SortedSet<String> all_pfams_encountered ) {
1543         final File all_pfams_encountered_file = new File( outfile_name + surfacing.ALL_PFAMS_ENCOUNTERED_SUFFIX );
1544         final File all_pfams_encountered_with_go_annotation_file = new File( outfile_name
1545                 + surfacing.ALL_PFAMS_ENCOUNTERED_WITH_GO_ANNOTATION_SUFFIX );
1546         final File encountered_pfams_summary_file = new File( outfile_name + surfacing.ENCOUNTERED_PFAMS_SUMMARY_SUFFIX );
1547         int biological_process_counter = 0;
1548         int cellular_component_counter = 0;
1549         int molecular_function_counter = 0;
1550         int pfams_with_mappings_counter = 0;
1551         int pfams_without_mappings_counter = 0;
1552         int pfams_without_mappings_to_bp_or_mf_counter = 0;
1553         int pfams_with_mappings_to_bp_or_mf_counter = 0;
1554         try {
1555             final Writer all_pfams_encountered_writer = new BufferedWriter( new FileWriter( all_pfams_encountered_file ) );
1556             final Writer all_pfams_encountered_with_go_annotation_writer = new BufferedWriter( new FileWriter( all_pfams_encountered_with_go_annotation_file ) );
1557             final Writer summary_writer = new BufferedWriter( new FileWriter( encountered_pfams_summary_file ) );
1558             summary_writer.write( "# Pfam to GO mapping summary" );
1559             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1560             summary_writer.write( "# Actual summary is at the end of this file." );
1561             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1562             summary_writer.write( "# Encountered Pfams without a GO mapping:" );
1563             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1564             for( final String pfam : all_pfams_encountered ) {
1565                 all_pfams_encountered_writer.write( pfam );
1566                 all_pfams_encountered_writer.write( ForesterUtil.LINE_SEPARATOR );
1567                 final DomainId domain_id = new DomainId( pfam );
1568                 if ( domain_id_to_go_ids_map.containsKey( domain_id ) ) {
1569                     ++pfams_with_mappings_counter;
1570                     all_pfams_encountered_with_go_annotation_writer.write( pfam );
1571                     all_pfams_encountered_with_go_annotation_writer.write( ForesterUtil.LINE_SEPARATOR );
1572                     final List<GoId> go_ids = domain_id_to_go_ids_map.get( domain_id );
1573                     boolean maps_to_bp = false;
1574                     boolean maps_to_cc = false;
1575                     boolean maps_to_mf = false;
1576                     for( final GoId go_id : go_ids ) {
1577                         final GoTerm go_term = go_id_to_term_map.get( go_id );
1578                         if ( go_term.getGoNameSpace().isBiologicalProcess() ) {
1579                             maps_to_bp = true;
1580                         }
1581                         else if ( go_term.getGoNameSpace().isCellularComponent() ) {
1582                             maps_to_cc = true;
1583                         }
1584                         else if ( go_term.getGoNameSpace().isMolecularFunction() ) {
1585                             maps_to_mf = true;
1586                         }
1587                     }
1588                     if ( maps_to_bp ) {
1589                         ++biological_process_counter;
1590                     }
1591                     if ( maps_to_cc ) {
1592                         ++cellular_component_counter;
1593                     }
1594                     if ( maps_to_mf ) {
1595                         ++molecular_function_counter;
1596                     }
1597                     if ( maps_to_bp || maps_to_mf ) {
1598                         ++pfams_with_mappings_to_bp_or_mf_counter;
1599                     }
1600                     else {
1601                         ++pfams_without_mappings_to_bp_or_mf_counter;
1602                     }
1603                 }
1604                 else {
1605                     ++pfams_without_mappings_to_bp_or_mf_counter;
1606                     ++pfams_without_mappings_counter;
1607                     summary_writer.write( pfam );
1608                     summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1609                 }
1610             }
1611             all_pfams_encountered_writer.close();
1612             all_pfams_encountered_with_go_annotation_writer.close();
1613             ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote all [" + all_pfams_encountered.size()
1614                     + "] encountered Pfams to: \"" + all_pfams_encountered_file + "\"" );
1615             ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote all [" + pfams_with_mappings_counter
1616                     + "] encountered Pfams with GO mappings to: \"" + all_pfams_encountered_with_go_annotation_file
1617                     + "\"" );
1618             ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote summary (including all ["
1619                     + pfams_without_mappings_counter + "] encountered Pfams without GO mappings) to: \""
1620                     + encountered_pfams_summary_file + "\"" );
1621             ForesterUtil.programMessage( surfacing.PRG_NAME, "Sum of Pfams encountered                : "
1622                     + all_pfams_encountered.size() );
1623             ForesterUtil.programMessage( surfacing.PRG_NAME, "Pfams without a mapping                 : "
1624                     + pfams_without_mappings_counter + " ["
1625                     + ( 100 * pfams_without_mappings_counter / all_pfams_encountered.size() ) + "%]" );
1626             ForesterUtil.programMessage( surfacing.PRG_NAME, "Pfams without mapping to proc. or func. : "
1627                     + pfams_without_mappings_to_bp_or_mf_counter + " ["
1628                     + ( 100 * pfams_without_mappings_to_bp_or_mf_counter / all_pfams_encountered.size() ) + "%]" );
1629             ForesterUtil.programMessage( surfacing.PRG_NAME,
1630                                          "Pfams with a mapping                    : " + pfams_with_mappings_counter
1631                                                  + " ["
1632                                                  + ( 100 * pfams_with_mappings_counter / all_pfams_encountered.size() )
1633                                                  + "%]" );
1634             ForesterUtil.programMessage( surfacing.PRG_NAME, "Pfams with a mapping to proc. or func.  : "
1635                     + pfams_with_mappings_to_bp_or_mf_counter + " ["
1636                     + ( 100 * pfams_with_mappings_to_bp_or_mf_counter / all_pfams_encountered.size() ) + "%]" );
1637             ForesterUtil.programMessage( surfacing.PRG_NAME,
1638                                          "Pfams with mapping to biological process: " + biological_process_counter
1639                                                  + " ["
1640                                                  + ( 100 * biological_process_counter / all_pfams_encountered.size() )
1641                                                  + "%]" );
1642             ForesterUtil.programMessage( surfacing.PRG_NAME,
1643                                          "Pfams with mapping to molecular function: " + molecular_function_counter
1644                                                  + " ["
1645                                                  + ( 100 * molecular_function_counter / all_pfams_encountered.size() )
1646                                                  + "%]" );
1647             ForesterUtil.programMessage( surfacing.PRG_NAME,
1648                                          "Pfams with mapping to cellular component: " + cellular_component_counter
1649                                                  + " ["
1650                                                  + ( 100 * cellular_component_counter / all_pfams_encountered.size() )
1651                                                  + "%]" );
1652             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1653             summary_writer.write( "# Sum of Pfams encountered                : " + all_pfams_encountered.size() );
1654             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1655             summary_writer.write( "# Pfams without a mapping                 : " + pfams_without_mappings_counter
1656                     + " [" + ( 100 * pfams_without_mappings_counter / all_pfams_encountered.size() ) + "%]" );
1657             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1658             summary_writer.write( "# Pfams without mapping to proc. or func. : "
1659                     + pfams_without_mappings_to_bp_or_mf_counter + " ["
1660                     + ( 100 * pfams_without_mappings_to_bp_or_mf_counter / all_pfams_encountered.size() ) + "%]" );
1661             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1662             summary_writer.write( "# Pfams with a mapping                    : " + pfams_with_mappings_counter + " ["
1663                     + ( 100 * pfams_with_mappings_counter / all_pfams_encountered.size() ) + "%]" );
1664             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1665             summary_writer.write( "# Pfams with a mapping to proc. or func.  : "
1666                     + pfams_with_mappings_to_bp_or_mf_counter + " ["
1667                     + ( 100 * pfams_with_mappings_to_bp_or_mf_counter / all_pfams_encountered.size() ) + "%]" );
1668             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1669             summary_writer.write( "# Pfams with mapping to biological process: " + biological_process_counter + " ["
1670                     + ( 100 * biological_process_counter / all_pfams_encountered.size() ) + "%]" );
1671             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1672             summary_writer.write( "# Pfams with mapping to molecular function: " + molecular_function_counter + " ["
1673                     + ( 100 * molecular_function_counter / all_pfams_encountered.size() ) + "%]" );
1674             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1675             summary_writer.write( "# Pfams with mapping to cellular component: " + cellular_component_counter + " ["
1676                     + ( 100 * cellular_component_counter / all_pfams_encountered.size() ) + "%]" );
1677             summary_writer.write( ForesterUtil.LINE_SEPARATOR );
1678             summary_writer.close();
1679         }
1680         catch ( final IOException e ) {
1681             ForesterUtil.printWarningMessage( surfacing.PRG_NAME, "Failure to write: " + e );
1682         }
1683     }
1684
1685     public static void writeBinaryDomainCombinationsFileForGraphAnalysis( final String[][] input_file_properties,
1686                                                                           final File output_dir,
1687                                                                           final GenomeWideCombinableDomains gwcd,
1688                                                                           final int i,
1689                                                                           final GenomeWideCombinableDomainsSortOrder dc_sort_order ) {
1690         File dc_outfile_dot = new File( input_file_properties[ i ][ 0 ]
1691                 + surfacing.DOMAIN_COMBINITONS_OUTPUTFILE_SUFFIX_FOR_GRAPH_ANALYSIS );
1692         if ( output_dir != null ) {
1693             dc_outfile_dot = new File( output_dir + ForesterUtil.FILE_SEPARATOR + dc_outfile_dot );
1694         }
1695         checkForOutputFileWriteability( dc_outfile_dot );
1696         final SortedSet<BinaryDomainCombination> binary_combinations = createSetOfAllBinaryDomainCombinationsPerGenome( gwcd );
1697         try {
1698             final BufferedWriter out_dot = new BufferedWriter( new FileWriter( dc_outfile_dot ) );
1699             for( final BinaryDomainCombination bdc : binary_combinations ) {
1700                 out_dot.write( bdc.toGraphDescribingLanguage( BinaryDomainCombination.OutputFormat.DOT, null, null )
1701                         .toString() );
1702                 out_dot.write( SurfacingConstants.NL );
1703             }
1704             out_dot.close();
1705         }
1706         catch ( final IOException e ) {
1707             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getMessage() );
1708         }
1709         ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote binary domain combination for \""
1710                 + input_file_properties[ i ][ 0 ] + "\" (" + input_file_properties[ i ][ 1 ] + ", "
1711                 + input_file_properties[ i ][ 2 ] + ") to: \"" + dc_outfile_dot + "\"" );
1712     }
1713
1714     public static void writeBinaryStatesMatrixAsListToFile( final CharacterStateMatrix<CharacterStateMatrix.GainLossStates> matrix,
1715                                                             final CharacterStateMatrix.GainLossStates state,
1716                                                             final String filename,
1717                                                             final String indentifier_characters_separator,
1718                                                             final String character_separator,
1719                                                             final Map<String, String> descriptions ) {
1720         final File outfile = new File( filename );
1721         checkForOutputFileWriteability( outfile );
1722         final SortedSet<String> sorted_ids = new TreeSet<String>();
1723         for( int i = 0; i < matrix.getNumberOfIdentifiers(); ++i ) {
1724             sorted_ids.add( matrix.getIdentifier( i ) );
1725         }
1726         try {
1727             final BufferedWriter out = new BufferedWriter( new FileWriter( outfile ) );
1728             for( final String id : sorted_ids ) {
1729                 out.write( indentifier_characters_separator );
1730                 out.write( "#" + id );
1731                 out.write( indentifier_characters_separator );
1732                 for( int c = 0; c < matrix.getNumberOfCharacters(); ++c ) {
1733                     // Not nice:
1734                     // using null to indicate either UNCHANGED_PRESENT or GAIN.
1735                     if ( ( matrix.getState( id, c ) == state )
1736                             || ( ( state == null ) && ( ( matrix.getState( id, c ) == CharacterStateMatrix.GainLossStates.GAIN ) || ( matrix
1737                                     .getState( id, c ) == CharacterStateMatrix.GainLossStates.UNCHANGED_PRESENT ) ) ) ) {
1738                         out.write( matrix.getCharacter( c ) );
1739                         if ( ( descriptions != null ) && !descriptions.isEmpty()
1740                                 && descriptions.containsKey( matrix.getCharacter( c ) ) ) {
1741                             out.write( "\t" );
1742                             out.write( descriptions.get( matrix.getCharacter( c ) ) );
1743                         }
1744                         out.write( character_separator );
1745                     }
1746                 }
1747             }
1748             out.flush();
1749             out.close();
1750         }
1751         catch ( final IOException e ) {
1752             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getMessage() );
1753         }
1754         ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote characters list: \"" + filename + "\"" );
1755     }
1756
1757     public static void writeBinaryStatesMatrixAsListToFileForBinaryCombinationsForGraphAnalysis( final CharacterStateMatrix<CharacterStateMatrix.GainLossStates> matrix,
1758                                                                                                  final CharacterStateMatrix.GainLossStates state,
1759                                                                                                  final String filename,
1760                                                                                                  final String indentifier_characters_separator,
1761                                                                                                  final String character_separator,
1762                                                                                                  final BinaryDomainCombination.OutputFormat bc_output_format ) {
1763         final File outfile = new File( filename );
1764         checkForOutputFileWriteability( outfile );
1765         final SortedSet<String> sorted_ids = new TreeSet<String>();
1766         for( int i = 0; i < matrix.getNumberOfIdentifiers(); ++i ) {
1767             sorted_ids.add( matrix.getIdentifier( i ) );
1768         }
1769         try {
1770             final BufferedWriter out = new BufferedWriter( new FileWriter( outfile ) );
1771             for( final String id : sorted_ids ) {
1772                 out.write( indentifier_characters_separator );
1773                 out.write( "#" + id );
1774                 out.write( indentifier_characters_separator );
1775                 for( int c = 0; c < matrix.getNumberOfCharacters(); ++c ) {
1776                     // Not nice:
1777                     // using null to indicate either UNCHANGED_PRESENT or GAIN.
1778                     if ( ( matrix.getState( id, c ) == state )
1779                             || ( ( state == null ) && ( ( matrix.getState( id, c ) == CharacterStateMatrix.GainLossStates.GAIN ) || ( matrix
1780                                     .getState( id, c ) == CharacterStateMatrix.GainLossStates.UNCHANGED_PRESENT ) ) ) ) {
1781                         BinaryDomainCombination bdc = null;
1782                         try {
1783                             bdc = BasicBinaryDomainCombination.createInstance( matrix.getCharacter( c ) );
1784                         }
1785                         catch ( final Exception e ) {
1786                             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getLocalizedMessage() );
1787                         }
1788                         out.write( bdc.toGraphDescribingLanguage( bc_output_format, null, null ).toString() );
1789                         out.write( character_separator );
1790                     }
1791                 }
1792             }
1793             out.flush();
1794             out.close();
1795         }
1796         catch ( final IOException e ) {
1797             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getMessage() );
1798         }
1799         ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote characters list: \"" + filename + "\"" );
1800     }
1801
1802     public static void writeBinaryStatesMatrixToList( final Map<DomainId, List<GoId>> domain_id_to_go_ids_map,
1803                                                       final Map<GoId, GoTerm> go_id_to_term_map,
1804                                                       final GoNameSpace go_namespace_limit,
1805                                                       final boolean domain_combinations,
1806                                                       final CharacterStateMatrix<CharacterStateMatrix.GainLossStates> matrix,
1807                                                       final CharacterStateMatrix.GainLossStates state,
1808                                                       final String filename,
1809                                                       final String indentifier_characters_separator,
1810                                                       final String character_separator,
1811                                                       final String title_for_html,
1812                                                       final String prefix_for_html,
1813                                                       final Map<DomainId, Set<String>>[] domain_id_to_secondary_features_maps,
1814                                                       final SortedSet<String> all_pfams_encountered,
1815                                                       final SortedSet<String> pfams_gained_or_lost,
1816                                                       final String suffix_for_per_node_events_file ) {
1817         if ( ( go_namespace_limit != null ) && ( ( go_id_to_term_map == null ) || ( go_id_to_term_map.size() < 1 ) ) ) {
1818             throw new IllegalArgumentException( "attempt to use GO namespace limit without a GO-id to term map" );
1819         }
1820         else if ( ( ( domain_id_to_go_ids_map == null ) || ( domain_id_to_go_ids_map.size() < 1 ) ) ) {
1821             throw new IllegalArgumentException( "attempt to output detailed HTML without a Pfam to GO map" );
1822         }
1823         else if ( ( ( go_id_to_term_map == null ) || ( go_id_to_term_map.size() < 1 ) ) ) {
1824             throw new IllegalArgumentException( "attempt to output detailed HTML without a GO-id to term map" );
1825         }
1826         final File outfile = new File( filename );
1827         checkForOutputFileWriteability( outfile );
1828         final SortedSet<String> sorted_ids = new TreeSet<String>();
1829         for( int i = 0; i < matrix.getNumberOfIdentifiers(); ++i ) {
1830             sorted_ids.add( matrix.getIdentifier( i ) );
1831         }
1832         try {
1833             final Writer out = new BufferedWriter( new FileWriter( outfile ) );
1834             final File per_node_go_mapped_domain_gain_loss_files_base_dir = createBaseDirForPerNodeDomainFiles( surfacing.BASE_DIRECTORY_PER_NODE_DOMAIN_GAIN_LOSS_FILES,
1835                                                                                                                 domain_combinations,
1836                                                                                                                 state,
1837                                                                                                                 filename );
1838             Writer per_node_go_mapped_domain_gain_loss_outfile_writer = null;
1839             File per_node_go_mapped_domain_gain_loss_outfile = null;
1840             int per_node_counter = 0;
1841             out.write( "<html>" );
1842             out.write( SurfacingConstants.NL );
1843             addHtmlHead( out, title_for_html );
1844             out.write( SurfacingConstants.NL );
1845             out.write( "<body>" );
1846             out.write( SurfacingConstants.NL );
1847             out.write( "<h1>" );
1848             out.write( SurfacingConstants.NL );
1849             out.write( title_for_html );
1850             out.write( SurfacingConstants.NL );
1851             out.write( "</h1>" );
1852             out.write( SurfacingConstants.NL );
1853             out.write( "<table>" );
1854             out.write( SurfacingConstants.NL );
1855             for( final String id : sorted_ids ) {
1856                 final Matcher matcher = PATTERN_SP_STYLE_TAXONOMY.matcher( id );
1857                 if ( matcher.matches() ) {
1858                     continue;
1859                 }
1860                 out.write( "<tr>" );
1861                 out.write( "<td>" );
1862                 out.write( "<a href=\"#" + id + "\">" + id + "</a>" );
1863                 out.write( "</td>" );
1864                 out.write( "</tr>" );
1865                 out.write( SurfacingConstants.NL );
1866             }
1867             out.write( "</table>" );
1868             out.write( SurfacingConstants.NL );
1869             for( final String id : sorted_ids ) {
1870                 final Matcher matcher = PATTERN_SP_STYLE_TAXONOMY.matcher( id );
1871                 if ( matcher.matches() ) {
1872                     continue;
1873                 }
1874                 out.write( SurfacingConstants.NL );
1875                 out.write( "<h2>" );
1876                 out.write( "<a name=\"" + id + "\">" + id + "</a>" );
1877                 writeTaxonomyLinks( out, id );
1878                 out.write( "</h2>" );
1879                 out.write( SurfacingConstants.NL );
1880                 out.write( "<table>" );
1881                 out.write( SurfacingConstants.NL );
1882                 out.write( "<tr>" );
1883                 out.write( "<td><b>" );
1884                 out.write( "Pfam domain(s)" );
1885                 out.write( "</b></td><td><b>" );
1886                 out.write( "GO term acc" );
1887                 out.write( "</b></td><td><b>" );
1888                 out.write( "GO term" );
1889                 out.write( "</b></td><td><b>" );
1890                 out.write( "GO namespace" );
1891                 out.write( "</b></td>" );
1892                 out.write( "</tr>" );
1893                 out.write( SurfacingConstants.NL );
1894                 out.write( "</tr>" );
1895                 out.write( SurfacingConstants.NL );
1896                 per_node_counter = 0;
1897                 if ( matrix.getNumberOfCharacters() > 0 ) {
1898                     per_node_go_mapped_domain_gain_loss_outfile = new File( per_node_go_mapped_domain_gain_loss_files_base_dir
1899                             + ForesterUtil.FILE_SEPARATOR + id + suffix_for_per_node_events_file );
1900                     SurfacingUtil.checkForOutputFileWriteability( per_node_go_mapped_domain_gain_loss_outfile );
1901                     per_node_go_mapped_domain_gain_loss_outfile_writer = ForesterUtil
1902                             .createBufferedWriter( per_node_go_mapped_domain_gain_loss_outfile );
1903                 }
1904                 else {
1905                     per_node_go_mapped_domain_gain_loss_outfile = null;
1906                     per_node_go_mapped_domain_gain_loss_outfile_writer = null;
1907                 }
1908                 for( int c = 0; c < matrix.getNumberOfCharacters(); ++c ) {
1909                     // Not nice:
1910                     // using null to indicate either UNCHANGED_PRESENT or GAIN.
1911                     if ( ( matrix.getState( id, c ) == state )
1912                             || ( ( state == null ) && ( ( matrix.getState( id, c ) == CharacterStateMatrix.GainLossStates.UNCHANGED_PRESENT ) || ( matrix
1913                                     .getState( id, c ) == CharacterStateMatrix.GainLossStates.GAIN ) ) ) ) {
1914                         final String character = matrix.getCharacter( c );
1915                         String domain_0 = "";
1916                         String domain_1 = "";
1917                         if ( character.indexOf( BinaryDomainCombination.SEPARATOR ) > 0 ) {
1918                             final String[] s = character.split( BinaryDomainCombination.SEPARATOR );
1919                             if ( s.length != 2 ) {
1920                                 throw new AssertionError( "this should not have happened: unexpected format for domain combination: ["
1921                                         + character + "]" );
1922                             }
1923                             domain_0 = s[ 0 ];
1924                             domain_1 = s[ 1 ];
1925                         }
1926                         else {
1927                             domain_0 = character;
1928                         }
1929                         writeDomainData( domain_id_to_go_ids_map,
1930                                          go_id_to_term_map,
1931                                          go_namespace_limit,
1932                                          out,
1933                                          domain_0,
1934                                          domain_1,
1935                                          prefix_for_html,
1936                                          character_separator,
1937                                          domain_id_to_secondary_features_maps,
1938                                          null );
1939                         all_pfams_encountered.add( domain_0 );
1940                         if ( pfams_gained_or_lost != null ) {
1941                             pfams_gained_or_lost.add( domain_0 );
1942                         }
1943                         if ( !ForesterUtil.isEmpty( domain_1 ) ) {
1944                             all_pfams_encountered.add( domain_1 );
1945                             if ( pfams_gained_or_lost != null ) {
1946                                 pfams_gained_or_lost.add( domain_1 );
1947                             }
1948                         }
1949                         if ( per_node_go_mapped_domain_gain_loss_outfile_writer != null ) {
1950                             writeDomainsToIndividualFilePerTreeNode( per_node_go_mapped_domain_gain_loss_outfile_writer,
1951                                                                      domain_0,
1952                                                                      domain_1 );
1953                             per_node_counter++;
1954                         }
1955                     }
1956                 }
1957                 if ( per_node_go_mapped_domain_gain_loss_outfile_writer != null ) {
1958                     per_node_go_mapped_domain_gain_loss_outfile_writer.close();
1959                     if ( per_node_counter < 1 ) {
1960                         per_node_go_mapped_domain_gain_loss_outfile.delete();
1961                     }
1962                     per_node_counter = 0;
1963                 }
1964                 out.write( "</table>" );
1965                 out.write( SurfacingConstants.NL );
1966                 out.write( "<hr>" );
1967                 out.write( SurfacingConstants.NL );
1968             } // for( final String id : sorted_ids ) {
1969             out.write( "</body>" );
1970             out.write( SurfacingConstants.NL );
1971             out.write( "</html>" );
1972             out.write( SurfacingConstants.NL );
1973             out.flush();
1974             out.close();
1975         }
1976         catch ( final IOException e ) {
1977             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getMessage() );
1978         }
1979         ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote characters detailed HTML list: \"" + filename + "\"" );
1980     }
1981
1982     public static void writeDomainCombinationsCountsFile( final String[][] input_file_properties,
1983                                                           final File output_dir,
1984                                                           final Writer per_genome_domain_promiscuity_statistics_writer,
1985                                                           final GenomeWideCombinableDomains gwcd,
1986                                                           final int i,
1987                                                           final GenomeWideCombinableDomains.GenomeWideCombinableDomainsSortOrder dc_sort_order ) {
1988         File dc_outfile = new File( input_file_properties[ i ][ 0 ]
1989                 + surfacing.DOMAIN_COMBINITON_COUNTS_OUTPUTFILE_SUFFIX );
1990         if ( output_dir != null ) {
1991             dc_outfile = new File( output_dir + ForesterUtil.FILE_SEPARATOR + dc_outfile );
1992         }
1993         checkForOutputFileWriteability( dc_outfile );
1994         try {
1995             final BufferedWriter out = new BufferedWriter( new FileWriter( dc_outfile ) );
1996             out.write( gwcd.toStringBuilder( dc_sort_order ).toString() );
1997             out.close();
1998         }
1999         catch ( final IOException e ) {
2000             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getMessage() );
2001         }
2002         final DescriptiveStatistics stats = gwcd.getPerGenomeDomainPromiscuityStatistics();
2003         try {
2004             per_genome_domain_promiscuity_statistics_writer.write( input_file_properties[ i ][ 0 ] + "\t" );
2005             per_genome_domain_promiscuity_statistics_writer.write( FORMATTER_3.format( stats.arithmeticMean() ) + "\t" );
2006             if ( stats.getN() < 2 ) {
2007                 per_genome_domain_promiscuity_statistics_writer.write( "n/a" + "\t" );
2008             }
2009             else {
2010                 per_genome_domain_promiscuity_statistics_writer.write( FORMATTER_3.format( stats
2011                         .sampleStandardDeviation() ) + "\t" );
2012             }
2013             per_genome_domain_promiscuity_statistics_writer.write( FORMATTER_3.format( stats.median() ) + "\t" );
2014             per_genome_domain_promiscuity_statistics_writer.write( ( int ) stats.getMin() + "\t" );
2015             per_genome_domain_promiscuity_statistics_writer.write( ( int ) stats.getMax() + "\t" );
2016             per_genome_domain_promiscuity_statistics_writer.write( stats.getN() + "\t" );
2017             final SortedSet<DomainId> mpds = gwcd.getMostPromiscuosDomain();
2018             for( final DomainId mpd : mpds ) {
2019                 per_genome_domain_promiscuity_statistics_writer.write( mpd.getId() + " " );
2020             }
2021             per_genome_domain_promiscuity_statistics_writer.write( ForesterUtil.LINE_SEPARATOR );
2022         }
2023         catch ( final IOException e ) {
2024             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getMessage() );
2025         }
2026         if ( input_file_properties[ i ].length == 3 ) {
2027             ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote domain combination counts for \""
2028                     + input_file_properties[ i ][ 0 ] + "\" (" + input_file_properties[ i ][ 1 ] + ", "
2029                     + input_file_properties[ i ][ 2 ] + ") to: \"" + dc_outfile + "\"" );
2030         }
2031         else {
2032             ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote domain combination counts for \""
2033                     + input_file_properties[ i ][ 0 ] + "\" (" + input_file_properties[ i ][ 1 ] + ") to: \""
2034                     + dc_outfile + "\"" );
2035         }
2036     }
2037
2038     private static void writeDomainData( final Map<DomainId, List<GoId>> domain_id_to_go_ids_map,
2039                                          final Map<GoId, GoTerm> go_id_to_term_map,
2040                                          final GoNameSpace go_namespace_limit,
2041                                          final Writer out,
2042                                          final String domain_0,
2043                                          final String domain_1,
2044                                          final String prefix_for_html,
2045                                          final String character_separator_for_non_html_output,
2046                                          final Map<DomainId, Set<String>>[] domain_id_to_secondary_features_maps,
2047                                          final Set<GoId> all_go_ids ) throws IOException {
2048         boolean any_go_annotation_present = false;
2049         boolean first_has_no_go = false;
2050         int domain_count = 2; // To distinguish between domains and binary domain combinations.
2051         if ( ForesterUtil.isEmpty( domain_1 ) ) {
2052             domain_count = 1;
2053         }
2054         // The following has a difficult to understand logic.
2055         for( int d = 0; d < domain_count; ++d ) {
2056             List<GoId> go_ids = null;
2057             boolean go_annotation_present = false;
2058             if ( d == 0 ) {
2059                 final DomainId domain_id = new DomainId( domain_0 );
2060                 if ( domain_id_to_go_ids_map.containsKey( domain_id ) ) {
2061                     go_annotation_present = true;
2062                     any_go_annotation_present = true;
2063                     go_ids = domain_id_to_go_ids_map.get( domain_id );
2064                 }
2065                 else {
2066                     first_has_no_go = true;
2067                 }
2068             }
2069             else {
2070                 final DomainId domain_id = new DomainId( domain_1 );
2071                 if ( domain_id_to_go_ids_map.containsKey( domain_id ) ) {
2072                     go_annotation_present = true;
2073                     any_go_annotation_present = true;
2074                     go_ids = domain_id_to_go_ids_map.get( domain_id );
2075                 }
2076             }
2077             if ( go_annotation_present ) {
2078                 boolean first = ( ( d == 0 ) || ( ( d == 1 ) && first_has_no_go ) );
2079                 for( final GoId go_id : go_ids ) {
2080                     out.write( "<tr>" );
2081                     if ( first ) {
2082                         first = false;
2083                         writeDomainIdsToHtml( out,
2084                                               domain_0,
2085                                               domain_1,
2086                                               prefix_for_html,
2087                                               domain_id_to_secondary_features_maps );
2088                     }
2089                     else {
2090                         out.write( "<td></td>" );
2091                     }
2092                     if ( !go_id_to_term_map.containsKey( go_id ) ) {
2093                         throw new IllegalArgumentException( "GO-id [" + go_id + "] not found in GO-id to GO-term map" );
2094                     }
2095                     final GoTerm go_term = go_id_to_term_map.get( go_id );
2096                     if ( ( go_namespace_limit == null ) || go_namespace_limit.equals( go_term.getGoNameSpace() ) ) {
2097                         // final String top = GoUtils.getPenultimateGoTerm( go_term, go_id_to_term_map ).getName();
2098                         final String go_id_str = go_id.getId();
2099                         out.write( "<td>" );
2100                         out.write( "<a href=\"" + SurfacingConstants.AMIGO_LINK + go_id_str
2101                                 + "\" target=\"amigo_window\">" + go_id_str + "</a>" );
2102                         out.write( "</td><td>" );
2103                         out.write( go_term.getName() );
2104                         if ( domain_count == 2 ) {
2105                             out.write( " (" + d + ")" );
2106                         }
2107                         out.write( "</td><td>" );
2108                         // out.write( top );
2109                         // out.write( "</td><td>" );
2110                         out.write( "[" );
2111                         out.write( go_term.getGoNameSpace().toShortString() );
2112                         out.write( "]" );
2113                         out.write( "</td>" );
2114                         if ( all_go_ids != null ) {
2115                             all_go_ids.add( go_id );
2116                         }
2117                     }
2118                     else {
2119                         out.write( "<td>" );
2120                         out.write( "</td><td>" );
2121                         out.write( "</td><td>" );
2122                         out.write( "</td><td>" );
2123                         out.write( "</td>" );
2124                     }
2125                     out.write( "</tr>" );
2126                     out.write( SurfacingConstants.NL );
2127                 }
2128             }
2129         } //  for( int d = 0; d < domain_count; ++d )
2130         if ( !any_go_annotation_present ) {
2131             out.write( "<tr>" );
2132             writeDomainIdsToHtml( out, domain_0, domain_1, prefix_for_html, domain_id_to_secondary_features_maps );
2133             out.write( "<td>" );
2134             out.write( "</td><td>" );
2135             out.write( "</td><td>" );
2136             out.write( "</td><td>" );
2137             out.write( "</td>" );
2138             out.write( "</tr>" );
2139             out.write( SurfacingConstants.NL );
2140         }
2141     }
2142
2143     private static void writeDomainIdsToHtml( final Writer out,
2144                                               final String domain_0,
2145                                               final String domain_1,
2146                                               final String prefix_for_detailed_html,
2147                                               final Map<DomainId, Set<String>>[] domain_id_to_secondary_features_maps )
2148             throws IOException {
2149         out.write( "<td>" );
2150         if ( !ForesterUtil.isEmpty( prefix_for_detailed_html ) ) {
2151             out.write( prefix_for_detailed_html );
2152             out.write( " " );
2153         }
2154         out.write( "<a href=\"" + SurfacingConstants.PFAM_FAMILY_ID_LINK + domain_0 + "\">" + domain_0 + "</a>" );
2155         out.write( "</td>" );
2156     }
2157
2158     public static DescriptiveStatistics writeDomainSimilaritiesToFile( final StringBuilder html_desc,
2159                                                                        final StringBuilder html_title,
2160                                                                        final Writer single_writer,
2161                                                                        Map<Character, Writer> split_writers,
2162                                                                        final SortedSet<DomainSimilarity> similarities,
2163                                                                        final boolean treat_as_binary,
2164                                                                        final List<Species> species_order,
2165                                                                        final PrintableDomainSimilarity.PRINT_OPTION print_option,
2166                                                                        final DomainSimilarity.DomainSimilaritySortField sort_field,
2167                                                                        final DomainSimilarity.DomainSimilarityScoring scoring,
2168                                                                        final boolean verbose ) throws IOException {
2169         final DescriptiveStatistics stats = new BasicDescriptiveStatistics();
2170         String histogram_title = null;
2171         switch ( sort_field ) {
2172             case ABS_MAX_COUNTS_DIFFERENCE:
2173                 if ( treat_as_binary ) {
2174                     histogram_title = "absolute counts difference:";
2175                 }
2176                 else {
2177                     histogram_title = "absolute (maximal) counts difference:";
2178                 }
2179                 break;
2180             case MAX_COUNTS_DIFFERENCE:
2181                 if ( treat_as_binary ) {
2182                     histogram_title = "counts difference:";
2183                 }
2184                 else {
2185                     histogram_title = "(maximal) counts difference:";
2186                 }
2187                 break;
2188             case DOMAIN_ID:
2189                 histogram_title = "score mean:";
2190                 break;
2191             case MIN:
2192                 histogram_title = "score minimum:";
2193                 break;
2194             case MAX:
2195                 histogram_title = "score maximum:";
2196                 break;
2197             case MAX_DIFFERENCE:
2198                 if ( treat_as_binary ) {
2199                     histogram_title = "difference:";
2200                 }
2201                 else {
2202                     histogram_title = "(maximal) difference:";
2203                 }
2204                 break;
2205             case MEAN:
2206                 histogram_title = "score mean:";
2207                 break;
2208             case SD:
2209                 histogram_title = "score standard deviation:";
2210                 break;
2211             case SPECIES_COUNT:
2212                 histogram_title = "species number:";
2213                 break;
2214             default:
2215                 throw new AssertionError( "Unknown sort field: " + sort_field );
2216         }
2217         for( final DomainSimilarity similarity : similarities ) {
2218             switch ( sort_field ) {
2219                 case ABS_MAX_COUNTS_DIFFERENCE:
2220                     stats.addValue( Math.abs( similarity.getMaximalDifferenceInCounts() ) );
2221                     break;
2222                 case MAX_COUNTS_DIFFERENCE:
2223                     stats.addValue( similarity.getMaximalDifferenceInCounts() );
2224                     break;
2225                 case DOMAIN_ID:
2226                     stats.addValue( similarity.getMeanSimilarityScore() );
2227                     break;
2228                 case MIN:
2229                     stats.addValue( similarity.getMinimalSimilarityScore() );
2230                     break;
2231                 case MAX:
2232                     stats.addValue( similarity.getMaximalSimilarityScore() );
2233                     break;
2234                 case MAX_DIFFERENCE:
2235                     stats.addValue( similarity.getMaximalDifference() );
2236                     break;
2237                 case MEAN:
2238                     stats.addValue( similarity.getMeanSimilarityScore() );
2239                     break;
2240                 case SD:
2241                     stats.addValue( similarity.getStandardDeviationOfSimilarityScore() );
2242                     break;
2243                 case SPECIES_COUNT:
2244                     stats.addValue( similarity.getSpecies().size() );
2245                     break;
2246                 default:
2247                     throw new AssertionError( "Unknown sort field: " + sort_field );
2248             }
2249         }
2250         //
2251         // final HistogramData[] hists = new HistogramData[ 1 ];
2252         //
2253         //
2254         // List<HistogramDataItem> data_items = new
2255         // ArrayList<HistogramDataItem>();
2256         // double[] values = stats.getDataAsDoubleArray();
2257         // for( int i = 0; i < values.length; i++ ) {
2258         // HistogramDataItem data_item = new BasicHistogramDataItem( "", values[
2259         // i ] );
2260         // data_items.add( data_item );
2261         // }
2262         //
2263         //
2264         // HistogramData hd0 = new HistogramData( "name",
2265         // data_items,
2266         // null, 20,
2267         // 40 );
2268         //
2269         //
2270         //
2271         //
2272         // hists[ 0 ] = hd0;
2273         //
2274         // final HistogramsFrame hf = new HistogramsFrame( hists );
2275         // hf.setVisible( true );
2276         //
2277         AsciiHistogram histo = null;
2278         if ( stats.getMin() < stats.getMin() ) {
2279             histo = new AsciiHistogram( stats, histogram_title );
2280         }
2281         if ( verbose ) {
2282             if ( histo != null ) {
2283                 System.out.println( histo.toStringBuffer( 20, '|', 40, 5 ) );
2284             }
2285             System.out.println();
2286             System.out.println( "N                   : " + stats.getN() );
2287             System.out.println( "Min                 : " + stats.getMin() );
2288             System.out.println( "Max                 : " + stats.getMax() );
2289             System.out.println( "Mean                : " + stats.arithmeticMean() );
2290             if ( stats.getN() > 1 ) {
2291                 System.out.println( "SD                  : " + stats.sampleStandardDeviation() );
2292             }
2293             else {
2294                 System.out.println( "SD                  : n/a" );
2295             }
2296             System.out.println( "Median              : " + stats.median() );
2297             if ( stats.getN() > 1 ) {
2298                 System.out.println( "Pearsonian skewness : " + stats.pearsonianSkewness() );
2299             }
2300             else {
2301                 System.out.println( "Pearsonian skewness : n/a" );
2302             }
2303         }
2304         if ( ( single_writer != null ) && ( ( split_writers == null ) || split_writers.isEmpty() ) ) {
2305             split_writers = new HashMap<Character, Writer>();
2306             split_writers.put( '_', single_writer );
2307         }
2308         switch ( print_option ) {
2309             case SIMPLE_TAB_DELIMITED:
2310                 break;
2311             case HTML:
2312                 for( final Character key : split_writers.keySet() ) {
2313                     final Writer w = split_writers.get( key );
2314                     w.write( "<html>" );
2315                     w.write( SurfacingConstants.NL );
2316                     if ( key != '_' ) {
2317                         addHtmlHead( w, "DCs (" + html_title + ") " + key.toString().toUpperCase() );
2318                     }
2319                     else {
2320                         addHtmlHead( w, "DCs (" + html_title + ")" );
2321                     }
2322                     w.write( SurfacingConstants.NL );
2323                     w.write( "<body>" );
2324                     w.write( SurfacingConstants.NL );
2325                     w.write( html_desc.toString() );
2326                     w.write( SurfacingConstants.NL );
2327                     w.write( "<hr>" );
2328                     w.write( "<br>" );
2329                     w.write( SurfacingConstants.NL );
2330                     w.write( "<tt><pre>" );
2331                     w.write( SurfacingConstants.NL );
2332                     if ( histo != null ) {
2333                         w.write( histo.toStringBuffer( 20, '|', 40, 5 ).toString() );
2334                         w.write( SurfacingConstants.NL );
2335                     }
2336                     w.write( "</pre></tt>" );
2337                     w.write( SurfacingConstants.NL );
2338                     w.write( "<table>" );
2339                     w.write( SurfacingConstants.NL );
2340                     w.write( "<tr><td>N: </td><td>" + stats.getN() + "</td></tr>" );
2341                     w.write( SurfacingConstants.NL );
2342                     w.write( "<tr><td>Min: </td><td>" + stats.getMin() + "</td></tr>" );
2343                     w.write( SurfacingConstants.NL );
2344                     w.write( "<tr><td>Max: </td><td>" + stats.getMax() + "</td></tr>" );
2345                     w.write( SurfacingConstants.NL );
2346                     w.write( "<tr><td>Mean: </td><td>" + stats.arithmeticMean() + "</td></tr>" );
2347                     w.write( SurfacingConstants.NL );
2348                     if ( stats.getN() > 1 ) {
2349                         w.write( "<tr><td>SD: </td><td>" + stats.sampleStandardDeviation() + "</td></tr>" );
2350                     }
2351                     else {
2352                         w.write( "<tr><td>SD: </td><td>n/a</td></tr>" );
2353                     }
2354                     w.write( SurfacingConstants.NL );
2355                     w.write( "<tr><td>Median: </td><td>" + stats.median() + "</td></tr>" );
2356                     w.write( SurfacingConstants.NL );
2357                     if ( stats.getN() > 1 ) {
2358                         w.write( "<tr><td>Pearsonian skewness: </td><td>" + stats.pearsonianSkewness() + "</td></tr>" );
2359                     }
2360                     else {
2361                         w.write( "<tr><td>Pearsonian skewness: </td><td>n/a</td></tr>" );
2362                     }
2363                     w.write( SurfacingConstants.NL );
2364                     w.write( "</table>" );
2365                     w.write( SurfacingConstants.NL );
2366                     w.write( "<br>" );
2367                     w.write( SurfacingConstants.NL );
2368                     w.write( "<hr>" );
2369                     w.write( SurfacingConstants.NL );
2370                     w.write( "<br>" );
2371                     w.write( SurfacingConstants.NL );
2372                     w.write( "<table>" );
2373                     w.write( SurfacingConstants.NL );
2374                 }
2375                 break;
2376         }
2377         for( final Writer w : split_writers.values() ) {
2378             w.write( SurfacingConstants.NL );
2379         }
2380         for( final DomainSimilarity similarity : similarities ) {
2381             if ( ( species_order != null ) && !species_order.isEmpty() ) {
2382                 ( ( PrintableDomainSimilarity ) similarity ).setSpeciesOrder( species_order );
2383             }
2384             if ( single_writer != null ) {
2385                 single_writer.write( similarity.toStringBuffer( print_option ).toString() );
2386             }
2387             else {
2388                 Writer local_writer = split_writers.get( ( similarity.getDomainId().getId().charAt( 0 ) + "" )
2389                         .toLowerCase().charAt( 0 ) );
2390                 if ( local_writer == null ) {
2391                     local_writer = split_writers.get( '0' );
2392                 }
2393                 local_writer.write( similarity.toStringBuffer( print_option ).toString() );
2394             }
2395             for( final Writer w : split_writers.values() ) {
2396                 w.write( SurfacingConstants.NL );
2397             }
2398         }
2399         switch ( print_option ) {
2400             case HTML:
2401                 for( final Writer w : split_writers.values() ) {
2402                     w.write( SurfacingConstants.NL );
2403                     w.write( "</table>" );
2404                     w.write( SurfacingConstants.NL );
2405                     w.write( "</font>" );
2406                     w.write( SurfacingConstants.NL );
2407                     w.write( "</body>" );
2408                     w.write( SurfacingConstants.NL );
2409                     w.write( "</html>" );
2410                     w.write( SurfacingConstants.NL );
2411                 }
2412                 break;
2413         }
2414         for( final Writer w : split_writers.values() ) {
2415             w.close();
2416         }
2417         return stats;
2418     }
2419
2420     private static void writeDomainsToIndividualFilePerTreeNode( final Writer individual_files_writer,
2421                                                                  final String domain_0,
2422                                                                  final String domain_1 ) throws IOException {
2423         individual_files_writer.write( domain_0 );
2424         individual_files_writer.write( ForesterUtil.LINE_SEPARATOR );
2425         if ( !ForesterUtil.isEmpty( domain_1 ) ) {
2426             individual_files_writer.write( domain_1 );
2427             individual_files_writer.write( ForesterUtil.LINE_SEPARATOR );
2428         }
2429     }
2430
2431     public static void writeMatrixToFile( final CharacterStateMatrix<?> matrix,
2432                                           final String filename,
2433                                           final Format format ) {
2434         final File outfile = new File( filename );
2435         checkForOutputFileWriteability( outfile );
2436         try {
2437             final BufferedWriter out = new BufferedWriter( new FileWriter( outfile ) );
2438             matrix.toWriter( out, format );
2439             out.flush();
2440             out.close();
2441         }
2442         catch ( final IOException e ) {
2443             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getMessage() );
2444         }
2445         ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote matrix: \"" + filename + "\"" );
2446     }
2447
2448     public static void writeMatrixToFile( final File matrix_outfile, final List<DistanceMatrix> matrices ) {
2449         checkForOutputFileWriteability( matrix_outfile );
2450         try {
2451             final BufferedWriter out = new BufferedWriter( new FileWriter( matrix_outfile ) );
2452             for( final DistanceMatrix distance_matrix : matrices ) {
2453                 out.write( distance_matrix.toStringBuffer( DistanceMatrix.Format.PHYLIP ).toString() );
2454                 out.write( ForesterUtil.LINE_SEPARATOR );
2455                 out.flush();
2456             }
2457             out.close();
2458         }
2459         catch ( final IOException e ) {
2460             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getMessage() );
2461         }
2462         ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote distance matrices to \"" + matrix_outfile + "\"" );
2463     }
2464
2465     private static void writePfamsToFile( final String outfile_name, final SortedSet<String> pfams ) {
2466         try {
2467             final Writer writer = new BufferedWriter( new FileWriter( new File( outfile_name ) ) );
2468             for( final String pfam : pfams ) {
2469                 writer.write( pfam );
2470                 writer.write( ForesterUtil.LINE_SEPARATOR );
2471             }
2472             writer.close();
2473             ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote " + pfams.size() + " pfams to [" + outfile_name
2474                     + "]" );
2475         }
2476         catch ( final IOException e ) {
2477             ForesterUtil.printWarningMessage( surfacing.PRG_NAME, "Failure to write: " + e );
2478         }
2479     }
2480
2481     public static void writePhylogenyToFile( final Phylogeny phylogeny, final String filename ) {
2482         final PhylogenyWriter writer = new PhylogenyWriter();
2483         try {
2484             writer.toPhyloXML( new File( filename ), phylogeny, 1 );
2485         }
2486         catch ( final IOException e ) {
2487             ForesterUtil.printWarningMessage( surfacing.PRG_NAME, "failed to write phylogeny to \"" + filename + "\": "
2488                     + e );
2489         }
2490         ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote phylogeny to \"" + filename + "\"" );
2491     }
2492
2493     public static void writeTaxonomyLinks( final Writer writer, final String species ) throws IOException {
2494         if ( ( species.length() > 1 ) && ( species.indexOf( '_' ) < 1 ) ) {
2495             final Matcher matcher = PATTERN_SP_STYLE_TAXONOMY.matcher( species );
2496             writer.write( " [" );
2497             if ( matcher.matches() ) {
2498                 writer.write( "<a href=\"" + SurfacingConstants.UNIPROT_LINK + species
2499                         + "\" target=\"taxonomy_window\">uniprot</a>" );
2500             }
2501             else {
2502                 writer.write( "<a href=\"" + SurfacingConstants.EOL_LINK + species
2503                         + "\" target=\"taxonomy_window\">eol</a>" );
2504                 writer.write( "|" );
2505                 writer.write( "<a href=\"" + SurfacingConstants.TOL_LINK + species
2506                         + "\" target=\"taxonomy_window\">tol</a>" );
2507             }
2508             writer.write( "]" );
2509         }
2510     }
2511
2512     private static void writeToNexus( final String outfile_name,
2513                                       final CharacterStateMatrix<BinaryStates> matrix,
2514                                       final Phylogeny phylogeny ) {
2515         if ( !( matrix instanceof BasicCharacterStateMatrix ) ) {
2516             throw new IllegalArgumentException( "can only write matrices of type [" + BasicCharacterStateMatrix.class
2517                     + "] to nexus" );
2518         }
2519         final BasicCharacterStateMatrix<BinaryStates> my_matrix = ( org.forester.evoinference.matrix.character.BasicCharacterStateMatrix<BinaryStates> ) matrix;
2520         final List<Phylogeny> phylogenies = new ArrayList<Phylogeny>( 1 );
2521         phylogenies.add( phylogeny );
2522         try {
2523             final BufferedWriter w = new BufferedWriter( new FileWriter( outfile_name ) );
2524             w.write( NexusConstants.NEXUS );
2525             w.write( ForesterUtil.LINE_SEPARATOR );
2526             my_matrix.writeNexusTaxaBlock( w );
2527             my_matrix.writeNexusBinaryChractersBlock( w );
2528             PhylogenyWriter.writeNexusTreesBlock( w, phylogenies, NH_CONVERSION_SUPPORT_VALUE_STYLE.NONE );
2529             w.flush();
2530             w.close();
2531             ForesterUtil.programMessage( surfacing.PRG_NAME, "Wrote Nexus file: \"" + outfile_name + "\"" );
2532         }
2533         catch ( final IOException e ) {
2534             ForesterUtil.fatalError( surfacing.PRG_NAME, e.getMessage() );
2535         }
2536     }
2537
2538     private static void writeToNexus( final String outfile_name,
2539                                       final DomainParsimonyCalculator domain_parsimony,
2540                                       final Phylogeny phylogeny ) {
2541         writeToNexus( outfile_name + surfacing.NEXUS_EXTERNAL_DOMAINS,
2542                       domain_parsimony.createMatrixOfDomainPresenceOrAbsence(),
2543                       phylogeny );
2544         writeToNexus( outfile_name + surfacing.NEXUS_EXTERNAL_DOMAIN_COMBINATIONS,
2545                       domain_parsimony.createMatrixOfBinaryDomainCombinationPresenceOrAbsence(),
2546                       phylogeny );
2547     }
2548
2549     public static void domainsPerProteinsStatistics( final String genome,
2550                                                      final List<Protein> protein_list,
2551                                                      final DescriptiveStatistics all_genomes_domains_per_potein_stats,
2552                                                      final SortedMap<Integer, Integer> all_genomes_domains_per_potein_histo,
2553                                                      final SortedSet<String> domains_which_are_always_single,
2554                                                      final SortedSet<String> domains_which_are_sometimes_single_sometimes_not,
2555                                                      final SortedSet<String> domains_which_never_single,
2556                                                      final Writer writer ) {
2557         final DescriptiveStatistics stats = new BasicDescriptiveStatistics();
2558         for( final Protein protein : protein_list ) {
2559             final int domains = protein.getNumberOfProteinDomains();
2560             //System.out.println( domains );
2561             stats.addValue( domains );
2562             all_genomes_domains_per_potein_stats.addValue( domains );
2563             if ( !all_genomes_domains_per_potein_histo.containsKey( domains ) ) {
2564                 all_genomes_domains_per_potein_histo.put( domains, 1 );
2565             }
2566             else {
2567                 all_genomes_domains_per_potein_histo.put( domains,
2568                                                           1 + all_genomes_domains_per_potein_histo.get( domains ) );
2569             }
2570             if ( domains == 1 ) {
2571                 final String domain = protein.getProteinDomain( 0 ).getDomainId().getId();
2572                 if ( !domains_which_are_sometimes_single_sometimes_not.contains( domain ) ) {
2573                     if ( domains_which_never_single.contains( domain ) ) {
2574                         domains_which_never_single.remove( domain );
2575                         domains_which_are_sometimes_single_sometimes_not.add( domain );
2576                     }
2577                     else {
2578                         domains_which_are_always_single.add( domain );
2579                     }
2580                 }
2581             }
2582             else if ( domains > 1 ) {
2583                 for( final Domain d : protein.getProteinDomains() ) {
2584                     final String domain = d.getDomainId().getId();
2585                     // System.out.println( domain );
2586                     if ( !domains_which_are_sometimes_single_sometimes_not.contains( domain ) ) {
2587                         if ( domains_which_are_always_single.contains( domain ) ) {
2588                             domains_which_are_always_single.remove( domain );
2589                             domains_which_are_sometimes_single_sometimes_not.add( domain );
2590                         }
2591                         else {
2592                             domains_which_never_single.add( domain );
2593                         }
2594                     }
2595                 }
2596             }
2597         }
2598         try {
2599             writer.write( genome );
2600             writer.write( "\t" );
2601             if ( stats.getN() >= 1 ) {
2602                 writer.write( stats.arithmeticMean() + "" );
2603                 writer.write( "\t" );
2604                 if ( stats.getN() >= 2 ) {
2605                     writer.write( stats.sampleStandardDeviation() + "" );
2606                 }
2607                 else {
2608                     writer.write( "" );
2609                 }
2610                 writer.write( "\t" );
2611                 writer.write( stats.median() + "" );
2612                 writer.write( "\t" );
2613                 writer.write( stats.getN() + "" );
2614                 writer.write( "\t" );
2615                 writer.write( stats.getMin() + "" );
2616                 writer.write( "\t" );
2617                 writer.write( stats.getMax() + "" );
2618             }
2619             else {
2620                 writer.write( "\t" );
2621                 writer.write( "\t" );
2622                 writer.write( "\t" );
2623                 writer.write( "0" );
2624                 writer.write( "\t" );
2625                 writer.write( "\t" );
2626             }
2627             writer.write( "\n" );
2628         }
2629         catch ( final IOException e ) {
2630             e.printStackTrace();
2631         }
2632     }
2633
2634     final static class DomainComparator implements Comparator<Domain> {
2635
2636         final private boolean _ascending;
2637
2638         public DomainComparator( final boolean ascending ) {
2639             _ascending = ascending;
2640         }
2641
2642         @Override
2643         public final int compare( final Domain d0, final Domain d1 ) {
2644
2645             if ( d0.getFrom() < d1.getFrom() ) {
2646                 return _ascending ? -1 : 1;
2647             }
2648             else if ( d0.getFrom() > d1.getFrom() ) {
2649                 return _ascending ? 1 : -1;
2650             }
2651             return 0;
2652
2653         }
2654
2655     }
2656 }
2657
2658
2659
2660
2661