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23 <title>Colouring by Conservation</title>
27 <strong>Colouring by Conservation</strong>
30 This is an approach to alignment colouring which highlights regions
31 of an alignment where physicochemical properties are conserved. It
32 is based on the one used in the AMAS method of multiple sequence
33 alignment analysis (Livingstone C.D. and Barton G.J. (1993), Protein
34 Sequence Alignments: A Strategy for the Hierarchical Analysis of
35 Residue Conservation.CABIOS Vol. 9 No. 6 (745-756)). See the <a
36 href="../calculations/conservation.html">conservation
37 calculation</a> help page for a more thorough explanation of the
40 <p>For an already coloured alignment, the conservation index at
41 each alignment position is used to modify the shading intensity of
42 the colour at that position. This means that the most conserved
43 columns in each group have the most intense colours, and the least
44 conserved are the palest. The slider controls the contrast between
47 Conservation can be calculated over all sequences in an alignment,
48 or just within specific groups (such as those defined by <a
49 href="../calculations/tree.html">phylogenetic tree
50 partitioning</a>). The option 'apply to all groups' controls whether
51 the contrast slider value will be applied to the indices for the
52 currently selected group, or all groups defined over the alignment.