3 * Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
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23 <title>Nucleic Acid Support</title>
24 <style type="text/css">
27 font-family: "Courier New", Courier, mono;
36 <strong>Nucleic Acid Support</strong>
39 <em>Colour Schemes</em>
41 <p>Jalview has color schemes for nucleic acid based sequences,
42 ability to fetch sequences from RFAM and RNA secondary structure
45 Information on the <a href="../colourSchemes/nucleotide.html">Nucleotide
46 colour scheme</a> and <a href="../colourSchemes/purinepyrimidine.html">
47 Purine/Pyrimidine colour scheme</a> are available under the Colour
48 Menu. See <a href="../colourSchemes/index.html">Colour Schemes</a>.
53 Jalview supports annotation of RNA sequences with secondary structure
54 information. You can interactively
55 <a href="../features/annotation.html#rna">create and edit RNA
56 secondary structure annotation rows</a>, or import data in the following
59 <li><em>RFAM</em> - Sequences can be <a
60 href="../features/seqfetch.html">fetched</a> from the RFAM
61 database by accession number or ID.</li>
62 <li><em>Stockholm files</em> - WUSS (or VIENNA) dot-bracket
63 notation found in the secondary structure annotation line will be
64 imported as sequence or alignment associated secondary structure
66 <li><em>Clustal files</em> - certain RNA alignment programs,
67 such as <a href="http://rna.informatik.uni-freiburg.de/LocARNA">LocaRNA</a>
68 output consensus RNA secondary structure lines in the line
69 normally reserved for the Clustal consensus line in a clustal
71 <li><em>RNAML</em> Jalview can import RNAML files containing
72 sequences and extended secondary structure annotation derived from
76 <strong>RNA Secondary Structure Visualization and Analysis</strong><br />
77 If a sequence or RNA alignment has secondary structure information,
78 the alignment will have a secondary structure line shown below it,
79 and a number of additional options become available:
81 <li><a href="../colourSchemes/rnahelicesColouring.html">RNA
82 Helix colouring</a> - highlights columns of alignment involved in
83 particular RNA helices, Uses the first displayed secondary
84 structure annotation.</li>
85 <li><a href="../calculations/structureconsensus.html">Base
86 Pair Conservation Analysis</a> - shown as a histogram and base-pair
87 logo below the alignment. Uses the first displayed secondary
88 structure annotation row.</li>
89 <li><a href="../features/varna.html">2D Structure
90 Visualization in VARNA</a> - allows linked viewing of the consensus
91 or an individual sequence's structure. Accessed via the Sequence
93 <li><strong>per sequence secondary structure
94 annotation</strong><br /> Sequence associated secondary structure
95 annotation imported via stockholm, PDB files, or other sources can
96 be shown on the alignment with the <strong>Colours→By
97 Annotation</strong> dialog box. Colours are assigned according to RNA
98 helix topology number (number of distinct nested helices).
99 Alignments can also be sorted by RNA helix secondary structure
100 topology number, <em>via</em> the <strong>Calculations→Sort→By
101 Annotation→Secondary Structure</strong> option (only present when
102 per-sequence secondary structure is available).</li>
105 <strong>Pseudo-knots</strong><br /> Jalview 2.8.2 introduced
106 limited support for working with structures including pseudoknots.
107 Where possible, extended WUSS symbols (e.g. different types of
108 parentheses, or upper and lower case letters) are preserved when
109 parsing RNA structure annotation and will be shaded differently when
110 displayed in the structure.<br /> Extended WUSS annotation is also
111 employed to distinguish different base pair interactions obtained
116 <strong>Limitations when working with RNA in Jalview</strong><br />
117 Currently, Jalview is not able to export RNA secondary structure
118 annotation in any format other than Jalview annotation </br> <em>Jalview's
119 RNA handling capabilities were introduced in v2.8</em>
121 <p align="center"> </p>