3 <head><title>The Alignment Annotations File</title></head>
\r
6 <p><strong>The Alignment Annotations File</strong></p>
\r
7 <p>Alignment annotations can be imported onto an alignment since
\r
8 version 2.08 of Jalview, via an annotations file. It is a simple
\r
9 ASCII text file consisting of tab
\r
10 delimited records similar to the <a
\r
11 href="featuresFormat.html">Sequence Features File</a>, and
\r
12 introduced primarily for use with the Jalview applet.</p>
\r
13 <p>Alignment annotations files are imported into Jalview in the
\r
16 <li>from the command line<strong><pre>
\r
17 -annotations <<em>Annotations filename</em>></pre></strong></li>
\r
18 <li>Dragging an annotations file onto an alignment window</li>
\r
19 <li>Via the "Load Features / Annotations" entry in the
\r
20 <strong>File</strong> menu of an alignment window.</li>
\r
23 <p><strong>Annotations File Format</strong></p>
\r
24 <p>The File consists of lines containing an instruction followed by
\r
25 tab delimited fields, and any lines starting with "#" are
\r
26 ignored. The first non-commented out line of a valid Annotations file must begin with :<strong><pre>JALVIEW_ANNOTATION</pre></strong></p>
\r
27 <p>A row of annotation is added with a line like
\r
28 <strong><pre><em>GRAPH_TYPE</em>	<em>Label</em>	<em>Values</em></pre></strong>
\r
30 <li><em>GRAPH_TYPE</em><br>This is can be either of "BAR_GRAPH",
\r
31 "LINE_GRAPH" or "NO_GRAPH", defining the type of
\r
32 annotation row that is being described.
\r
34 <li><em>Values</em><br>This field is a series
\r
35 of symbols and/or quantities separated by the pipe symbol "|",
\r
36 mapped onto the columns of the alignment.<br>Some types of annotation
\r
37 can display both text, symbols and quantities at each column, and these are
\r
38 separated with commas within the pipe-delimited Value field. Jalview
\r
39 will display the content if it's values are appropriate for the type
\r
40 of the annotation being defined:<ul>
\r
41 <li>BAR_GRAPH<br><em>text
\r
42 character</em>,<em>number</em></li>
\r
43 <li>LINE_GRAPH<br><em>number</em></li>
\r
44 <li>NO_GRAPH<br><em>text label</em>,<em>H or E for helix or strand
\r
45 symbol</em></li></ul>
\r
47 <p>You can associate an annotation with a sequence by preceding its
\r
48 definition with the line:
\r
49 <strong><pre>SEQUENCE_REF	<em>seq_name</em>	<em>[startIndex]</em></pre></strong>
\r
50 All Annotations defined after a SEQUENCE_REF command will then be
\r
51 associated with that sequence, and the first field in the Value
\r
52 field list will (optionally) be
\r
53 placed at the <em>startIndex</em>'th column.</p><p>Sequence associations
\r
54 are turned off for subsequent annotation definitions by:
\r
55 <strong><pre>SEQUENCE_REF	ALIGNMENT</pre></strong></p>
\r
56 <p><em>LINE_GRAPH</em> type annotations can be given a colour,
\r
57 combined onto the same vertical axis, and have abscissa (lines of
\r
58 constant value - <em>so they are horizontal</em>) at particular
\r
59 values using the following lines (respectively):<!--</p>
\r
60 <p><font size="3" face="Courier New, Courier, mono">-->
\r
61 <strong><pre>COLOUR	graph name	colour
\r
62 COMBINE	graph 1 name	graph 2 name
\r
63 GRAPHLINE	graph name	value	label	colour</pre></strong></p>
\r
64 <p>An example Annotation file is given below:</p>
\r
65 <p><font size="3" face="Courier New, Courier, mono">#Comment lines follow the hash symbol<br>
\r
66 JALVIEW_ANNOTATION<br>
\r
67 SEQUENCE_REF FER1_MESCR 5<br>
\r
68 BAR_GRAPH	Bar Graph 1	||-100,-|-200,-|-300,-|-400,-|200,+|300,+|150,+<br>
\r
69 LINE_GRAPH	Green Values	1.1|2.2|1.3|3.4|0.7|1.4|3.3|2.2|2.1|-1.1|3.2<br>
\r
70 LINE_GRAPH	Red Values	2.1|3.2|1.3|-1.4|5.5|1.4|1.3|4.2|-1.1|1.1|3.2<br>
\r
71 BAR_GRAPH	Bar Graph	2 1,.|2,*|3,:|4,.|5,*|4,:|3,.|2|1|1|2|3|4|5|4<br>
\r
72 NO_GRAPH	Icons 	||||E,Sheet1|E|E||||H,Sheet 2|H|H|H||||||<br>
\r
73 NO_GRAPH	Purple Letters	m|y|p|r|o|t|e|i|n</font></p>
\r
74 <p><font size="3" face="Courier New, Courier, mono">COLOUR	Bar Graph 2	blue<br>
\r
75 COLOUR	Red Values	255,0,0<br>
\r
76 COLOUR	Green Values	green<br>
\r
77 COLOUR	Purple Letters	151,52,228<br>
\r
78 COMBINE	Green Values	Red Values</font></p>
\r
79 <p><font size="3" face="Courier New, Courier, mono">GRAPHLINE	Red Values	2.6	threshold	black
\r