2 <head><title>Sequence Features</title></head>
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4 <p><strong>Sequence Features</strong></p>
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5 <p>Jalview can colour parts of a sequence based on the presence of
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6 sequence features - which may be retrieved from database records (such
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7 as Uniprot), the result of <a href="search.html">sequence motif
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8 searches</a> or simply read from a <a
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9 href="featuresFormat.html">sequence features file</a>.</p>
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10 <p><strong>Sequence Feature Groups</strong></p>
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11 <p>Since Jalview 2.08, sequence features assigned to a sequence can be
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12 organised into groups, which may indicate that the features were all retrieved
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13 from the same database (such as Uniprot features), or generated by the
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14 same analysis process (as might be specified in a <a
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15 href="featuresFormat.html">sequence features file</a>).</p>
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16 <p><strong>Sequence Feature Inheritance</strong></p>
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17 <p>Since Jalview 2.08, sequence features are <em>global</em> to a set of
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18 sequences appearing (independently or together) in many different
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19 alignments. Practically, this means features loaded onto one alignment
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20 can be viewed in any alignments involving the same sequences. The same
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21 sequence appears in different alignments when a new alignment is
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22 generated by submitting an existing set of sequences to one of the
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23 alignment or prediction web services, and when sequences are copied
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24 and pasted into other alignment windows.</p>
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25 <p><strong>View→Show Sequence Features</strong></p>
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26 <p>Toggle the display of sequence features in this alignment. If
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27 feature retrieval has not already been carried out, then Jalview will
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28 automatically try to fetch sequence features (as described below).</p>
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29 <p><strong>View→Sequence Feature Settings...</strong>
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30 <p>Once sequence features have been loaded, their display can be fully
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31 controlled using the alignment window's <a
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32 href="featuresettings.html">Sequence Feature Settings</a> dialog
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33 box. Feature colour schemes and display parameters are unique to a
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34 particular alignment, so it is possible to colour the same sequence
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35 features differently in different alignment views.</p>
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36 <p><strong>View→Fetch Sequence Features</strong></p>
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37 <p>When this option is selected, sequence features extracted from the
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38 <a href="http://www.ebi.uniprot.org/index.html">Uniprot (http://www.ebi.unprot.org/index.html)</a>
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39 record for each sequence are displayed on the alignment.</p>
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40 <p>Jalview will attempt to retrieve sequence features from Uniprot files using
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41 the EBI dbFetch web service using the given sequence names (or
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42 Uniprot ID, if available). A 100% match with
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43 the Uniprot record is required for Uniprot features to be view on a sequence.</p>
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44 <p>More information about the feature is given in a tooltip, which is viewed by
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45 moving the mouse pointer over a sequence feature. The description associated
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46 with the feature will then be displayed in a small label near the pointer.</p>
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47 <p><strong>The Sequence Identification Process</strong>
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50 <p>The first step in the procedure for matching uniprot IDs to
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51 sequences is to use the ID (name) of
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52 each sequence to retrieve Uniprot records directly.</p>
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54 If a uniprot record (or set of records) is found for a sequence,
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55 then the sequence is aligned to the one in the Uniprot record
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56 to determine the correct start and end residue positions (which are
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57 displayed when the 'Show Full Sequence ID' option is set).
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60 <p>If the alignment reveals differences between the sequence in the
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61 alignment and the one in the record, then Jalview will assume that
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62 the aligned sequence is not the one in the uniprot record.
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67 In some cases, the ID used to retrieve Uniprot records may be out of
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68 date and you will be notified of that a 100% match between the
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69 sequence and a Uniprot record was identified, but the sequence name
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70 must be manually changed (by right clicking on the sequence ID and selecting
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71 <strong>Sequence→Edit Name</strong>), before Jalview will show its sequence
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74 <li>remember to save your alignment if you have updated any of the sequence
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77 <p>Precalculated Sequence Features may be added to an alignment from the command
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78 line, drag and drop, or from the "File->Load Features / Annotations"
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79 menu item. See the <a href="featuresFormat.html">Features File Format</a> for
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