3 * Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
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11 * of the License, or (at your option) any later version.
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14 * WITHOUT ANY WARRANTY; without even the implied warranty
15 * of MERCHANTABILITY or FITNESS FOR A PARTICULAR
16 * PURPOSE. See the GNU General Public License for more details.
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20 * The Jalview Authors are detailed in the 'AUTHORS' file.
23 <title>Alignment Window Menus</title>
27 <!-- NOTE: THIS PAGE COLLECTS TOGETHER THE INDIVIDUAL ALIGNMENT WINDOW MENU PAGES - DON"T EDIT INDIVIDUAL ENTRIES HERE! -->
29 <strong>Alignment Window Menus</strong>
32 <li><strong>File</strong>
34 <li><strong>Fetch Sequence</strong><br> <em>Shows
35 a dialog window in which you can retrieve known ids from
36 UniProt, EMBL, EMBLCDS, PFAM, Rfam, or PDB database using
37 Web Services provided by the European Bioinformatics
38 Institute. See <a href="../features/seqfetch.html">Sequence
41 <li><strong>Add Sequences</strong><em><br> Add
42 sequences to the visible alignment from file, URL, or cut
43 & paste window </em></li>
44 <li><strong>Reload</strong><em><br> Reloads the
45 alignment from the original file, if available.<br> <strong>Warning:
46 This will delete any edits, analyses and colourings
47 applied since the alignment was last saved, and cannot be
48 undone.</strong> </em></li>
49 <li><strong>Save (Control S)</strong><em><br>
50 Saves the alignment to the file it was loaded from (if
51 available), in the same format, updating the original in
53 <li><strong>Save As (Control Shift S)<br>
54 </strong><em>Save the alignment to local file. A file selection
55 window will open, use the "Files of type:"
56 selection box to determine which <a href="../io/index.html">alignment
57 format</a> to save as.
59 <li><strong>Output to Textbox<br>
60 </strong><em>The alignment will be displayed in plain text in a new
61 window, which you can "Copy and Paste" using the
62 pull down menu, or your standard operating system copy and
63 paste keys. The output window also has a <strong>"New
64 Window"</strong> button to import the (possibly edited) text
65 as a new alignment.<br> Select the format of the text
66 by selecting one of the following menu items.
69 <li><strong>FASTA</strong></li>
70 <li><strong>MSF</strong></li>
71 <li><strong>CLUSTAL</strong></li>
72 <li><strong>BLC</strong></li>
73 <li><strong>PIR</strong></li>
74 <li><strong>PFAM</strong></li>
75 <li><strong>PileUp</strong></li>
76 <li><strong>AMSA</strong></li>
77 <li><strong>STH</strong></li>
78 <li><strong>Phylip</strong></li>
79 <li><strong>JSON</strong></li>
81 <li><strong>Page Setup ...</strong><br> <em>Open
82 the printing system's Page Setup dialog box, to control page
83 size, layout and orientation.</em></li>
84 <li><strong>Print (Control P)<br>
85 </strong><em>Jalview will print the alignment using the current
86 fonts and colours of your alignment. If the alignment has
87 annotations visible, these will be printed below the
88 alignment. If the alignment is wrapped the number of
89 residues per line of your alignment will depend on the paper
90 width or your alignment window width, whichever is the
92 <li><strong>Export Image</strong> <em><br>
93 Creates an alignment graphic with the current view's
94 annotation, alignment background colours and group colours.
95 If the alignment is <a href="../features/wrap.html">wrapped</a>,
96 the output will also be wrapped and will have the same
97 visible residue width as the open alignment. </em>
100 </strong><em>Create a <a href="../io/export.html">web page</a>
104 </strong><em>Create an <a href="../io/export.html">Encapsulated
105 Postscript</a> file from your alignment.
108 </strong><em>Create a <a href="../io/export.html">Portable
109 Network Graphics</a> file from your alignment.
112 </strong><em>Create a <a href="../io/export.html">Scalable
113 Vector Graphics</a> file from your alignment for embedding
116 <li><strong>BioJS<br>
117 </strong><em>Create a <a href="../io/export.html">BioJS MSA
118 Viewer HTML </a> file from your alignment.
121 <li><strong>Export Features</strong><em><br> All
122 features visible on the alignment can be saved to file or
123 displayed in a textbox in either Jalview or GFF format</em></li>
124 <li><strong>Export Annotations</strong><em><br>
125 All annotations visible on the alignment can be saved to
126 file or displayed in a textbox in Jalview annotations
128 <li><strong>Load Associated Tree<br>
129 </strong><em>Jalview can <a href="../calculations/treeviewer.html">view
130 trees</a> stored in the Newick file format, and associate them
131 with the alignment. Note: the ids of the tree file and your
132 alignment MUST be the same.
134 <li><strong>Load Features / Annotations<br>
135 </strong><em>Load files describing precalculated <a
136 href="../features/featuresFormat.html">sequence
137 features</a> or <a href="../features/annotationsFormat.html">alignment
140 <li><strong>Close (Control W)</strong><br> <em>Close
141 the alignment window. Make sure you have saved your
142 alignment before you close - either from the Desktop's <strong>Save
143 Project</strong> File menu option, or by using the <strong>Save
147 <li><strong>Edit</strong>
149 <li><strong>Undo (Control Z)</strong><em><br>
150 This will undo any edits you make to the alignment. This
151 applies to insertion or deletion of gaps, cutting residues
152 or sequences from the alignment or pasting sequences to the
153 current alignment or sorting the alignment. <strong>NOTE:</strong>
154 It DOES NOT undo colour changes, adjustments to group sizes,
155 or changes to the annotation panel. </em></li>
156 <li><strong>Redo (Control Y)<br>
157 </strong><em>Any actions which you undo can be redone using redo. </em></li>
158 <li><strong>Cut (Control X)<br>
159 </strong><em>This will make a copy of the currently selected
160 residues before removing them from your alignment. Click on
161 a sequence name if you wish to select a whole sequence. <br>
162 Use <CTRL> and X (<APPLE> and X on MacOSX) to
165 <li><strong>Copy (Control C)</strong><br> <em>Copies
166 the currently selected residues to the system clipboard -
167 you can also do this by pressing <CTRL> and C
168 (<APPLE> and C on MacOSX). <br> If you try to
169 paste the clipboard contents to a text editor, you will see
170 the format of the copied residues FASTA.
172 <li><strong>Paste </strong>
174 <li><strong>To New Alignment (Control Shift V)<br>
175 </strong><em>A new alignment window will be created from
176 sequences previously copied or cut to the system
177 clipboard. <br> Use <CTRL> and <SHIFT>
178 and V(<APPLE> and <SHIFT;> and and V on
181 <li><strong>Add To This Alignment (Control V)<br>
182 </strong><em>Copied sequences from another alignment window can
183 be added to the current Jalview alignment. </em></li>
185 <li><strong>Delete (Backspace)<br>
186 </strong><em>This will delete the currently selected residues
187 without copying them to the clipboard. Like the other edit
188 operations, this can be undone with <strong>Undo</strong>.
190 <li><strong>Remove Left (Control L)<br>
191 </strong><em>If the alignment has marked columns, the alignment will
192 be trimmed to the left of the leftmost marked column. To
193 mark a column, mouse click the scale bar above the
194 alignment. Click again to unmark a column, or select
195 "Deselect All" to deselect all columns.</em></li>
196 <li><strong>Remove Right (Control R)<br>
197 </strong><em>If the alignment has marked columns, the alignment will
198 be trimmed to the right of the rightmost marked column. To
199 mark a column, mouse click the scale bar above the
200 alignment. Click again to unmark a column, or select
201 "Deselect All" to deselect all columns.</em></li>
202 <li><strong>Remove Empty Columns (Control E)<br>
203 </strong><em>All columns which only contain gap characters
204 ("-", ".") will be deleted.<br> You
205 may set the default gap character in <a
206 href="../features/preferences.html">preferences</a>.
208 <li><strong>Remove All Gaps (Control Shift E)</strong><br>
209 <em>Gap characters ("-", ".") will be
210 deleted from the selected area of the alignment. If no
211 selection is made, ALL the gaps in the alignment will be
212 removed.<br> You may set the default gap character in <a
213 href="../features/preferences.html">preferences</a>.
215 <li><strong>Remove Redundancy (Control D)<br>
216 </strong><em>Selecting this option brings up a window asking you to
217 select a threshold. If the percentage identity between any
218 two sequences (under the current alignment) exceeds this
219 value then one of the sequences (the shorter) is discarded.
220 Press the "Apply" button to remove redundant
221 sequences. The "Undo" button will undo the last
222 redundancy deletion.</em></li>
223 <li><strong>Pad Gaps<br>
224 </strong><em>When selected, the alignment will be kept at minimal
225 width (so there are no empty columns before or after the
226 first or last aligned residue) and all sequences will be
227 padded with gap characters before and after their
228 terminating residues.<br> This switch is useful when
229 making a tree using unaligned sequences and when working
230 with alignment analysis programs which require 'properly
231 aligned sequences' to be all the same length.<br> You
232 may set the default for <strong>Pad Gaps</strong> in the <a
233 href="../features/preferences.html">preferences</a>.
236 <li><strong>Select</strong>
238 <li><a href="../features/search.html"><strong>Find...
239 (Control F)</strong></a><br> <em>Opens the Find dialog box to
240 search for residues, sequence name or residue position
241 within the alignment and create new sequence features from
243 <li><strong>Select All (Control A)</strong><strong><br>
244 </strong><em>Selects all the sequences and residues in the
245 alignment. <br> Use <CTRL> and A (<APPLE>
246 and A on a MacOSX) to select all.
248 <li><strong>Deselect All (Escape)<br>
249 </strong><em>Removes the current selection box (red dashed box) from
250 the alignment window. All selected sequences, residues and
251 marked columns will be deselected. </em><em> <br> Use
252 <ESCAPE> to deselect all.
254 <li><strong>Invert Sequence Selection (Control I)<br>
255 </strong><em>Any sequence ids currently not selected will replace
256 the current selection. </em></li>
257 <li><strong>Invert Column Selection (Control Alt
259 </strong><em>Any columns currently not selected will replace the
260 current column selection. </em></li>
261 <li><strong>Create Group (Control G)<br></strong> <em>Create
262 a group containing the currently selected sequences.</em></li>
263 <li><strong>Remove Group (Shift Control G)<br></strong>
264 <em>Ungroup the currently selected sequence group.</em></li>
265 <li><strong>Make Groups for selection<br /></strong> <em>The
266 currently selected groups of the alignment will be
267 subdivided according to the contents of the currently
268 selected region. <br />Use this to subdivide an alignment
269 based on the different combinations of residues at marked
272 <li><strong>Undefine Groups (Control U)<br>
273 </strong><em>The alignment will be reset with no defined groups.<br>
274 <strong>WARNING</strong>: This cannot be undone.
277 href="../features/columnFilterByAnnotation.html">Select/Hide
278 Columns by Annotation</a></strong> <br /> <em>Select or Hide
279 columns in the alignment according to secondary structure,
280 labels and values shown in alignment annotation rows. </em></li>
281 <li><strong>Select Highlighted Columns</strong> <br /> <em>Selects
282 the columns currently highlighted as a result of a find, mouse
283 over, or selection event from a linked structure viewer or other
284 application. Modifiers will work on some platforms: ALT will add
285 all but the highlighted set to the column selection, and CTRL
286 (or META) will toggle the selection. </em></li>
288 <li><strong>View</strong>
290 <li><strong>New View (Control T)</strong><em><br>
291 Creates a new view from the current alignment view. </em></li>
292 <li><strong>Expand Views (X)</strong><em><br>
293 Display each view associated with the alignment in its own
294 alignment window, allowing several views to be displayed
295 simultaneously. </em></li>
296 <li><strong>Gather Views (G)</strong><em><br>
297 Each view associated with the alignment will be displayed
298 within its own tab on the current alignment window. </em></li>
299 <li><strong>Show→(all Columns / Sequences /
300 Sequences and Columns)</strong><em><br> All hidden Columns
301 / Sequences / Sequences and Columns will be revealed. </em></li>
302 <li><strong>Hide→(all Columns / Sequences /
303 Selected Region / All but Selected Region )</strong><em><br>
304 Hides the all the currently selected Columns / Sequences /
305 Region or everything but the selected Region.</em></li>
306 <li><strong>Automatic Scrolling<br>
307 </strong><em>When selected, the view will automatically scroll to
308 display the highlighted sequence position corresponding to
309 the position under the mouse pointer in a linked alignment
310 or structure view.</em></li>
311 <li><strong>Show Sequence Features</strong><br> <em>Show
312 or hide sequence features on this alignment.</em></li>
314 href="../features/featuresettings.html">Sequence
315 Feature Settings...</a> </strong><em><br> <em>Opens the
316 Sequence Feature Settings dialog box to control the colour
317 and display of sequence features on the alignment, and
318 configure and retrieve features from DAS annotation
320 <li><strong>Sequence ID Tooltip</strong><em>
321 (application only) <br>This submenu's options allow the
322 inclusion or exclusion of non-positional sequence features
323 or database cross references from the tooltip shown when the
324 mouse hovers over the sequence ID panel.
326 <li><strong>Alignment Properties...<br />
327 </strong><em>Displays some simple statistics computed for the
328 current alignment view and any named properties defined on
329 the whole alignment.</em></li>
330 <li><strong><a href="../features/overview.html">Overview
331 Window</a><br> </strong><em>A scaled version of the alignment
332 will be displayed in a small window. A red box will indicate
333 the currently visible area of the alignment. Move the
334 visible region using the mouse. </em></li>
336 <li><strong>Annotations</strong><em> (Since Jalview 2.8.2)</em>
338 <li><strong>Show Annotations<br>
339 </strong><em>If this is selected the "Annotation Panel"
340 will be displayed below the alignment. The default setting
341 is to display the conservation calculation, quality
342 calculation and consensus values as bar charts. </em></li>
343 <li><strong>Show Alignment Related</strong><em><br>
344 Show all annotations that are for the alignment as a whole
345 (for example, Consensus, or secondary structure prediction
346 from alignment).</em></li>
347 <li><strong>Hide Alignment Related</strong><em><br>
348 Hide all annotations that are for the alignment as a whole.</em></li>
349 <li><strong>Show Sequence Related</strong><em><br>
350 Show all annotations that are for individual sequences.</em></li>
351 <li><strong>Hide Sequence Related</strong><em><br>
352 Hide all annotations that are for individual sequences.</em></li>
353 <li><em>You can also selectively show or hide
354 annotations from the <a href="./popupMenu.html">Popup</a> or
355 <a href="../features/annotation.html">Annotation</a> menus.
357 <li><strong>Sort by Sequence</strong><em><br>Sort
358 sequence-specific annotations by sequence order in the
359 alignment (and within that, by label).</em></li>
360 <li><strong>Sort by Label</strong><em><br>Sort
361 sequence-specific annotations by label (and within that, by
362 sequence order). If neither sort order is selected, no
363 sorting is applied, allowing you to make a manual ordering
364 of the annotations.</em></li>
365 <li><strong>Autocalculated Annotation<br>
366 </strong><em>Settings for the display of autocalculated annotation.</em>
368 <li><strong>Show first<br></strong><em> Show
369 autocalculated annotations above sequence-specific
370 annotations. Note this also applies to other annotations
371 for the alignment, for example secondary structure
372 prediction from alignment.</em></li>
373 <li><strong>Show last<br></strong><em> Show
374 autocalculated / alignment annotations below
375 sequence-specific annotations.</em></li>
376 <li><strong>Apply to all groups<br>
377 </strong><em> When ticked, any modification to the current
378 settings will be applied to all autocalculated
379 annotation.</em></li>
380 <li><strong>Show Consensus Histogram<br>
381 </strong><em> Enable or disable the display of the histogram
382 above the consensus sequence.</em></li>
383 <li><strong>Show Consensus Logo<br>
384 </strong><em> Enable or disable the display of the Consensus
385 Logo above the consensus sequence.</em></li>
386 <li><strong>Normalise Consensus Logo<br>
387 </strong><em>When enabled, scales all logo stacks to the same
388 height, making it easier to compare symbol diversity in
389 highly variable regions.</em></li>
390 <li><strong>Group Conservation<br>
391 </strong><em> When ticked, display a conservation row for all
392 groups (only available for protein alignments).</em></li>
393 <li><strong>Group Consensus<br>
394 </strong><em> When ticked, display a consensus row for all
398 <li><strong>Alignment Window Format Menu</strong>
400 <li><strong>Font...<br>
401 </strong><em>Opens the "Choose Font" dialog box, in order
402 to change the font of the display and enable or disable
403 'smooth fonts' (anti-aliasing) for faster alignment
404 rendering. </em></li>
406 </strong><em>When ticked, the alignment display is "<a
407 href="../features/wrap.html">wrapped</a>" to
408 the width of the alignment window. This is useful if your
409 alignment has only a few sequences to view its full width at
411 </em><br> Additional options for display of sequence numbering
412 and scales are also visible in wrapped layout mode:<br>
414 <li><strong>Scale Above</strong><br> <em>
415 Show the alignment column position scale.</em></li>
416 <li><strong>Scale Left</strong><br> <em> Show
417 the sequence position for the first aligned residue in
418 each row in the left column of the alignment.</em></li>
419 <li><strong>Scale Right</strong><br> <em>
420 Show the sequence position for the last aligned residue
421 in each row in the right-most column of the alignment.</em></li>
422 <li><strong>Show Sequence Limits<br>
423 </strong><em>If this box is selected the sequence name will have
424 the start and end position of the sequence appended to
425 the name, in the format NAME/START-END</em></li>
426 <li><strong>Right Align Sequence ID<br>
427 </strong><em>If this box is selected then the sequence names
428 displayed in the sequence label area will be aligned
429 against the left-hand edge of the alignment display,
430 rather than the left-hand edge of the alignment window.
432 <li><strong>Show Hidden Markers<br>
433 </strong><em>When this box is selected, positions in the
434 alignment where rows and columns are hidden will be
435 marked by blue arrows. </li>
436 <li><strong>Boxes</strong><em><br> If this is
437 selected the background of a residue will be coloured
438 using the selected background colour. Useful if used in
439 conjunction with "Colour Text." </em></li>
441 </strong><em>If this is selected the residues will be displayed
442 using the standard 1 character amino acid alphabet.</em></li>
443 <li><strong>Colour Text<br>
444 </strong><em>If this is selected the residues will be coloured
445 according to the background colour associated with that
446 residue. The colour is slightly darker than background
447 so the amino acid symbol remains visible. </em></li>
448 <li><strong>Show Gaps<br>
449 </strong><em>When this is selected, gap characters will be
450 displayed as "." or "-". If
451 unselected, then gap characters will appear as blank
452 spaces. <br> You may set the default gap character
453 in <a href="../features/preferences.html">preferences</a>.
455 <li><strong>Centre Annotation Labels<br>
456 </strong><em>Select this to center labels along an annotation
457 row relative to their associated column (default is off,
458 i.e. left-justified).</em></li>
459 <li><strong>Show Unconserved<br>
460 </strong><em>When this is selected, all consensus sequence
461 symbols will be rendered as a '.', highlighting
462 mutations in highly conserved alignments. </em></li>
470 <li><strong>Colour</strong>
472 <li><strong>Apply Colour To All Groups<br>
473 </strong><em>If this is selected, any changes made to the background
474 colour will be applied to all currently defined groups.<br>
477 href="../colourSchemes/textcolour.html">Colour
478 Text...</a> </strong><em><br> Opens the Colour Text dialog box
479 to set a different text colour for light and dark background,
480 and the intensity threshold for transition between them. </em></li>
481 <li>Colour Scheme options: <strong>None, ClustalX,
482 Blosum62 Score, Percentage Identity, Zappo, Taylor,
483 Hydrophobicity, Helix Propensity, Strand Propensity, Turn
484 Propensity, Buried Index, Nucleotide, Purine/Pyrimidine, User
486 </strong> <em>See <a href="../colourSchemes/index.html">colours</a>
487 for a description of all colour schemes.
489 <li><strong>By Conservation<br>
490 </strong><em>See <a href="../colourSchemes/conservation.html">Colouring
493 <li><strong>Modify Conservation Threshold<br>
494 </strong><em>Use this to display the conservation threshold slider
495 window. Useful if the window has been closed, or if the 'by
496 conservation' option appears to be doing nothing!</em><br></li>
497 <li><strong>Above Identity Threshold<br>
498 </strong><em>See <a href="../colourSchemes/abovePID.html">Above
499 Percentage Identity</a>
502 <li><strong>Modify Identity Threshold<br>
503 </strong><em>Use this to set the threshold value for colouring above
504 Identity. Useful if the window has been closed.<br>
506 <li><strong>By Annotation</strong><br> <em>Colours
507 the alignment on a per-column value from a specified
509 href="../colourSchemes/annotationColouring.html">Annotation
512 <li><strong>By RNA Helices</strong><br> <em>Colours
513 the helices of an RNA alignment loaded from a Stockholm file.
514 See <a href="../colourSchemes/rnahelicesColouring.html">RNA
515 Helices Colouring</a>.
518 <li><strong>Calculate</strong>
520 <li><strong>Sort </strong>
522 <li><strong>by ID</strong><em><br> This will
523 sort the sequences according to sequence name. If the sort
524 is repeated, the order of the sorted sequences will be
526 <li><strong>by Length</strong><em><br> This
527 will sort the sequences according to their length
528 (excluding gap characters). If the sort is repeated, the
529 order of the sorted sequences will be inverted. </em></li>
530 <li><strong>by Group</strong><strong><br> </strong><em>This
531 will sort the sequences according to sequence name. If the
532 sort is repeated, the order of the sorted sequences will
533 be inverted. </em><strong></strong></li>
534 <li><strong>by Pairwise Identity<br>
535 </strong><em>This will sort the selected sequences by their
536 percentage identity to the consensus sequence. The most
537 similar sequence is put at the top. </em></li>
538 <li><em>The <a href="../calculations/sorting.html">Sort
539 menu</a> will have some additional options if you have just
540 done a multiple alignment calculation, or opened a tree
544 <li><strong>Calculate Tree </strong> <br> <em>Functions
545 for calculating trees on the alignment or the currently
546 selected region. See <a href="../calculations/tree.html">calculating
550 <li><strong>Neighbour Joining Using PAM250 </strong></li>
551 <li><strong>Neighbour Joining Using Sequence
552 Feature Similarity</strong></li>
553 <li><strong>Neighbour Joining Using Blosum62 </strong></li>
554 <li><strong>Neighbour Joining Using % Identity</strong></li>
555 <li><strong>Average Distance Using PAM250 </strong></li>
556 <li><strong>Average Distance Using Sequence
557 Feature Similarity</strong></li>
558 <li><strong>Average Distance Using Blosum62</strong></li>
559 <li><strong>Average Distance Using % Identity</strong></li>
560 </ul> <strong>Note: Since Version 2.8.1, a number of
561 additional similarity measures for tree calculation are
562 provided in this menu.</strong></li>
563 <li><strong>Pairwise Alignments</strong><br> <em>Applies
564 Smith and Waterman algorithm to selected sequences. See <a
565 href="../calculations/pairwise.html">pairwise
568 <li><strong>Principal Component Analysis</strong><br> <em>Shows
569 a spatial clustering of the sequences based on similarity
570 scores calculated with the alignment. See <a
571 href="../calculations/pca.html">Principal
572 Component Analysis</a>.
574 <li><strong>Extract Scores ... (optional)</strong><br> <em>This
575 option is only visible if Jalview detects one or more
576 white-space separated values in the description line of the
577 alignment sequences.<br> When selected, these numbers are
578 parsed into sequence associated annotation which can then be
579 used to sort the alignment via the Sort by→Score menu.
581 <li><strong>Autocalculate Consensus</strong><br> <em>For
582 large alignments it can be useful to deselect
583 "Autocalculate Consensus" when editing. This
584 prevents the sometimes lengthy calculations performed after
585 each sequence edit.</em> <br></li>
586 <li><strong>Sort With New Tree</strong><br> <em>When
587 enabled, Jalview will automatically sort the alignment when a
588 new tree is calculated or loaded onto it.</em> <br></li>
589 <li><strong>Show Flanking Regions</strong><br> <em>Opens
590 a new alignment window showing any additional sequence data
591 either side of the current alignment. Useful in conjunction
592 with 'Fetch Database References' when the 'Trim Retrieved
593 Sequences' option is disabled to retrieve full length
594 sequences for a set of aligned peptides. </em></li>
597 <li><strong>Web Service Menu</strong><br /> <em>This menu
598 is dynamic, and may contain user-defined web service entries in
599 addition to any of the following ones:</em>
601 <li><strong>Fetch DB References</strong><br> <em>This
602 submenu contains options for accessing any of the database
603 services that Jalview is aware of (e.g. DAS sequence servers
604 and the WSDBFetch service provided by the EBI) to verify
605 sequence start/end positions and retrieve all database cross
606 references and PDB ids associated with all or just the
607 selected sequences in the alignment.
609 <li>'Trim Retrieved Sequences' - when checked, Jalview
610 will discard any additional sequence data for accessions
611 associated with sequences in the alignment. <br> <strong>Note:
612 Disabling this could cause out of memory errors when
613 working with genomic sequence records !</strong><br> <strong>Added
614 in Jalview 2.8.1</strong>
616 <li>'Standard Databases' will check sequences against
617 the EBI databases plus any active DAS sequence sources<</li>
618 </ul> Other sub-menus allow you to pick a specific source to query
619 - sources are listed alphabetically according to their
623 <p>Selecting items from the following submenus will start a
624 remote service on compute facilities at the University of Dundee,
625 or elsewhere. You need a continuous network connection in order to
626 use these services through Jalview.</p>
628 <li><strong>Alignment</strong><br /> <em> Align the
629 currently selected sequences or all sequences in the
630 alignment, or re-align unaligned sequences to the aligned
631 sequences. Entries in this menu provide access to the various
632 alignment programs supported by <a
633 href="../webServices/JABAWS.html">JABAWS</a>. See the
634 <a href="../webServices/msaclient.html">Multiple Sequence
635 Alignment webservice client</a> entry for more information.
637 <li><strong>Secondary Structure Prediction</strong>
639 <li><strong>JPred Secondary Structure Prediction</strong><br>
640 <em>Secondary structure prediction by network
641 consensus. See the <a href="../webServices/jnet.html">Jpred</a>
642 client entry for more information. The behaviour of this
643 calculation depends on the current selection:
645 <li>If nothing is selected, and the displayed
646 sequences appear to be aligned, then a JPred prediction
647 will be run for the first sequence in the alignment,
648 using the current alignment. Otherwise the first
649 sequence will be submitted for prediction.</li>
650 <li>If just one sequence (or a region on one
651 sequence) has been selected, it will be submitted to
652 the automatic JPred prediction server for homolog
653 detection and prediction.</li>
654 <li>If a set of sequences are selected, and they
655 appear to be aligned, then the alignment will be used
656 for a JPred prediction on the <strong>first</strong>
657 sequence in the set (that is, the one that appears
658 first in the alignment window).
663 <li><strong>Analysis</strong><br />
665 <li><strong>Multi-Harmony</strong><br> <em>Performs
666 functional residue analysis on a protein family alignment
667 with sub-families defined on it. See the <a
668 href="../webServices/shmr.html">Multi-Harmony
669 service</a> entry for more information.