1 #summary generalized speciation duplication inference
3 = Generalized Speciation Duplication Inference =
7 To infer duplication events on a gene tree given a trusted species tree.
12 path/to/forester.jar org.forester.application.gsdi [-options] <gene tree in phyloXML format> <species tree> <outfile>
16 * -g: to allow stripping of gene tree nodes without a matching species in the species tree
18 * -m: use most parimonious duplication model for GSDI: assign nodes as speciations which would otherwise be assiged as potential duplications due tp polytomies in the species tree
20 * -q: to allow species tree in other formats than phyloXML (i.e. Newick, NHX, Nexus)
22 * -b: to use SDIse algorithm instead of GSDI algorithm (for binary species trees)
25 Must be in phyloXM format, with taxonomy and sequence data in appropriate fields.
27 ==== Species tree ====
28 Must be in phyloXML format unless option -q is used.
32 Besides the main output of a gene tree with duplications and speciations assigned to all of its internal nodes, this program also produces the following:
33 * a log file, ending in `"_gsdi_log.txt"`
34 * a species tree file which only contains external nodes with were needed for the reconciliation, ending in `"_species_tree_used.xml"`
35 * if the gene tree contains species with scientific species names such as "Pyrococcus horikoshii strain ATCC 700860" and if a mapping cannot be establish bases on these, GSDI will attempt to map by removing the "strain" (or "subspecies") information, these will be listed in a file ending in `"_gsdi_remapped.txt"`.
38 `gsdi -g -q gene_tree.xml tree_of_life.nwk out.xml`
44 Zmasek CM and Eddy SR "A simple algorithm to infer gene duplication and speciation events on a gene tree" [http://bioinformatics.oxfordjournals.org/content/17/9/821.abstract Bioinformatics, 17, 821-828]
50 Download forester.jar here: http://code.google.com/p/forester/downloads/list