+ <li><strong>File</strong> \r
+ <ul>\r
+ <li><strong>Fetch Sequence</strong><br>\r
+ <em>Shows a dialog window in which you can select known ids from Uniprot, \r
+ EMBL, EMBLCDS or PDB database using Web Services provided by the European \r
+ Bioinformatics Institute. See <a href="features/seqfetch.html">Sequence \r
+ Fetcher</a></em>.</li>\r
+ <li><strong>Save As<br>\r
+ </strong><em>Save the alignment to local file. A file selection window \r
+ will open, use the "Files of type:" selection box to determine \r
+ which <a href="../io/index.html">alignment format</a> to save as.</em></li>\r
+ <li><strong>Export</strong> <em><br>\r
+ Creates an alignment graphic with the current annotation, alignment background \r
+ colours and group colours. If the alignment is <a\r
+ href="../features/wrap.html">wrapped</a>, the output will also be wrapped \r
+ and will have the same visible residue width as the open alignment. </em> \r
+ <ul>\r
+ <li><strong>HTML<br>\r
+ </strong><em>Create a <a href="../io/index.html#export">web page</a> \r
+ from your alignment.</em></li>\r
+ <li><strong>EPS<br>\r
+ </strong><em>Create an <a href="../io/index.html#export">Encapsulated \r
+ Postscript</a> file from your alignment.</em></li>\r
+ <li><strong>PNG<br>\r
+ </strong><em>Create a <a href="../io/index.html#export">Portable Network \r
+ Graphics</a> file from your alignment.</em></li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Output to Textbox<br>\r
+ </strong><em>The alignment will be displayed in plain text in a new window \r
+ which you can "Copy and Paste" using the pull down menu, or \r
+ your standard operating system copy and paste keys. <br>\r
+ Select the format of the text by selecting one of the following menu items.</em> \r
+ <ul>\r
+ <li><strong>FASTA</strong> <em></em></li>\r
+ <li><strong>MSF</strong></li>\r
+ <li><strong>CLUSTAL</strong></li>\r
+ <li><strong>BLC</strong></li>\r
+ <li><strong>PIR</strong></li>\r
+ <li><strong>PFAM</strong></li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Print<br>\r
+ </strong><em>Jalview will print the alignment using the current fonts \r
+ and colours of your alignment. If the alignment has annotations visible, \r
+ these will be printed below the alignment. If the alignment is wrapped \r
+ the number of residues per line of your alignment will depend on the paper \r
+ width or your alignment window width, whichever is the smaller. </em></li>\r
+ <li><strong>Load Associated Tree<br>\r
+ </strong><em>Jalview can <a\r
+ href="../calculations/treeviewer.html">view trees</a> stored in the Newick \r
+ file format, and associate them with the alignment. Note: the ids of the \r
+ tree file and your alignment MUST be the same.</em></li>\r
+ <li><strong>Close<br>\r
+ </strong><em>Close the alignment window. Make sure you have saved your \r
+ alignment before you close - either as a Jalview project or by using the \r
+ <strong>Save As</strong> menu.<br>\r
+ </em></li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Edit</strong> \r
+ <ul>\r
+ <li><strong>Undo</strong><em><br>\r
+ This will undo any edits you make to the alignment. This applies to insertion \r
+ or deletion of gaps, cutting residues or sequences from the alignment \r
+ or pasting sequences to the current alignment or sorting the alignment. \r
+ <strong>NOTE:</strong> It DOES NOT undo colour changes, adjustments to \r
+ group sizes, or changes to the annotation panel. </em></li>\r
+ <li><strong>Redo<br>\r
+ </strong><em>Any actions which you undo can be redone using redo. </em></li>\r
+ <li><strong>Cut<br>\r
+ </strong><em>This will make a copy of the currently selected residues \r
+ before removing them from your alignment. Click on a sequence name if \r
+ you wish to select a whole sequence. <br>\r
+ Use <CTRL> and X (<APPLE> and X on MacOSX) to cut.</em></li>\r
+ <li><strong>Copy</strong><br>\r
+ <em>Copies the currently selected residues to the system clipboard - you \r
+ can also do this by pressing <CTRL> and C (<APPLE> and C on \r
+ MacOSX). <br>\r
+ If you try to paste the clipboard contents to a text editor, you will \r
+ see the format of the copied residues FASTA.</em></li>\r
+ <li><strong>Paste </strong> \r
+ <ul>\r
+ <li><strong>To New Alignment<br>\r
+ </strong><em>A new alignment window will be created from sequences \r
+ previously copied or cut to the system clipboard. <br>\r
+ Use <CTRL> and V(<APPLE> and V on MacOSX) to paste.</em></li>\r
+ <li><strong>Add To This Alignment<br>\r
+ </strong><em>Copied sequences from another alignment window can be \r
+ added to the current Jalview alignment. </em></li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Delete<br>\r
+ </strong><em>This will delete the currently selected residues without \r
+ copying them to the clipboard. Like the other edit operations, this can \r
+ be undone with <strong>Undo</strong>.</em></li>\r
+ <li><strong>Select All<br>\r
+ </strong><em>Selects all the sequences and residues in the alignment. \r
+ <br>\r
+ Use <CTRL> and A (<APPLE> and A on a MacOSX) to select all.</em></li>\r
+ <li><strong>Deselect All<br>\r
+ </strong><em>Removes the current selection box (red dashed box) from the \r
+ alignment window. All selected sequences, residues and marked columns \r
+ will be deselected. </em><em> <br>\r
+ Use <ESCAPE> to deselect all.</em></li>\r
+ <li><strong>Invert Selection<br>\r
+ </strong><em>Any sequence ids currently not selected will replace the \r
+ current selection. </em></li>\r
+ <li><strong>Undefine Groups<br>\r
+ </strong><em>The alignment will be reset with no defined groups.<br>\r
+ <strong>WARNING</strong>: This cannot be undone.</em></li>\r
+ <li><strong>Remove Left<br>\r
+ </strong><em>If the alignment has marked columns, the alignment will be \r
+ trimmed to the left of the leftmost marked column. To mark a column, mouse \r
+ click the scale bar above the alignment. Click again to unmark a column, \r
+ or select "Deselect All" to deselect all columns.</em></li>\r
+ <li><strong>Remove Right<br>\r
+ </strong><em>If the alignment has marked columns, the alignment will be \r
+ trimmed to the left of the leftmost marked column. To mark a column, mouse \r
+ click the scale bar above the alignment. Click again to unmark a column, \r
+ or select "Deselect All" to deselect all columns.</em></li>\r
+ <li><strong>Remove Empty Columns<br>\r
+ </strong><em>All columns which only contain gap characters ("-", \r
+ ".") will be deleted.<br>\r
+ You may set the default gap character in <a href="../features/preferences.html">preferences</a>. \r
+ </em></li>\r
+ <li><strong>Remove All Gaps</strong><br>\r
+ <em>Gap characters ("-", ".") will be deleted from \r
+ the selected area of the alignment. If no selection is made, ALL the gaps \r
+ in the alignment will be removed.<br>\r
+ You may set the default gap character in <a href="../features/preferences.html">preferences</a>. \r
+ </em> </li>\r
+ <li><strong>Remove Redundancy<br>\r
+ </strong><em>Selecting this option brings up a window asking you to select \r
+ a threshold. If the percentage identity between any two sequences (under \r
+ the current alignment) exceeds this value then one of the sequences (the \r
+ shorter) is discarded. Press the "Apply" button to remove redundant \r
+ sequences. The "Undo" button will undo the last redundancy deletion.</em></li>\r
+ <li><strong>Pad Gaps<br>\r
+ </strong><em>Adds gaps to the end of all the sequences so they are all \r
+ the same length. This is useful for making a tree using unaligned sequences.<br>\r
+ You may set the default gap character in <a href="../features/preferences.html">preferences</a>. \r
+ </em><br>\r
+ </li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Search</strong> \r
+ <ul>\r
+ <li><strong>Find<br>\r
+ </strong><em>Select this to <a href="../features/search.html">search</a> \r
+ for residues, sequence name or residue position within the alignment. \r
+ <br>\r
+ </em></li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>View</strong> \r
+ <ul>\r
+ <li><strong>Font<br>\r
+ </strong><em>Change the font of the display from the "Choose Font" \r
+ dialog box, which is shown when this item is selected. </em></li>\r
+ <li><strong>Wrap<br>\r
+ </strong><em>When ticked, the alignment display is "<a href="../features/wrap.html">wrapped</a>" \r
+ to the width of the alignment window. This is useful if your alignment \r
+ has only a few sequences to view its full width at once.<br>\r
+ Options are available to show the residue numbering at the start and/or \r
+ end of an alignment as well as showing the alignment position above each \r
+ sequence row. <br>\r
+ <strong>NOTE</strong>: When in wrapped alignment view, the alignment cannot \r
+ be edited or have regions selected on it. </em></li>\r
+ <li><strong>Show Full Sequence ID<br>\r
+ </strong><em>If this box is selected the sequence name will have the start \r
+ and end position of the sequence appended to the name, in the format NAME/START-END</em></li>\r
+ <li><strong>Boxes</strong><em><br>\r
+ If this is selected the background of a residue will be coloured using \r
+ the selected background colour. Useful if used in conjunction with "Colour \r
+ Text." </em></li>\r
+ <li><strong>Text<br>\r
+ </strong><em>If this is selected the residues will be displayed using \r
+ the standard 1 character amino acid alphabet.</em></li>\r
+ <li><strong>Colour Text<br>\r
+ </strong><em>If this is selected the residues will be coloured according \r
+ to the background colour associated with that residue. The colour is slightly \r
+ darker than background so the amino acid symbol remains visible. </em></li>\r
+ <li><strong>Show Gaps<br>\r
+ </strong><em>When this is selected, gap characters will be displayed as \r
+ "." or "-". If unselected, then gap characters will \r
+ appear as blank spaces. <br>\r
+ You may set the default gap character in <a href="../features/preferences.html">preferences</a>.</em></li>\r
+ <li><strong>Show Annotations<br>\r
+ </strong><em>If this is selected the "Annotation Panel" will \r
+ be displayed below the alignment. The default setting is to display the \r
+ conservation calculation, quality calculation and consensus values as \r
+ bar charts. </em></li>\r
+ <li><strong>Sequence Features<br>\r
+ </strong><em>If the sequence names are Swissprot entries Jalview will \r
+ use the names to retrieve <a href="../features/seqfeatures.html">sequence \r
+ features</a> from the EBI. Features which are 1 residue in length are \r
+ coloured red, sequences longer than 1 residue are coloured blue. Move \r
+ the mouse over a coloured feature to display the details of the feature. \r
+ <br>\r
+ Note: The retrieved information will update the sequence start and end \r
+ labels if they are incorrect. </em></li>\r
+ <li><strong>Seqence Settings </strong><br>\r
+ <em>If features have been added to the alignment then the priority of \r
+ rendering the features can be altered so that overlapping features can \r
+ be displayed or hidden. See <a href="features/seqfeatures.html">Sequence \r
+ Features</a>.</em></li>\r
+ <li><strong><a href="../features/overview.html">Overview Window</a><br>\r
+ </strong><em>A scaled version of the alignment will be displayed in a \r
+ small window. A red box will indicate the currently visible area of the \r
+ alignment. Move the visible region using the mouse. </em><strong> </strong></li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Colour</strong> \r
+ <ul>\r
+ <li><strong>Apply Colour To All Groups<br>\r
+ </strong><em>If this is selected, any changes made to the background colour \r
+ will be applied to all currently defined groups.</em></li>\r
+ <li>Colour Scheme options: <strong>None, ClustalX, Blosum62 Score, Percentage \r
+ Identity, Zappo, Taylor, Hydrophobicity, Helix Propensity, Strand Propensity, \r
+ Turn Propensity, Buried Index, Nucleotide, User Defined<br>\r
+ </strong> <em>See <a href="../colourSchemes/index.html">colours</a> for \r
+ a description of all colour schemes.</em></li>\r
+ <li><strong>By Conservation<br>\r
+ </strong><em>See <a href="../colourSchemes/conservation.html">Colouring \r
+ by Conservation</a>.</em></li>\r
+ <li><strong>Modify Conservation Threshold<br>\r
+ </strong><em>Use this to display the conservation threshold slider window. \r
+ Useful if the window has been closed, or if the 'by conservation' option \r
+ appears to be doing nothing!</em></li>\r
+ <li><strong>Above Identity Threshold<br>\r
+ </strong><em>See <a href="../colourSchemes/abovePID.html">Above Percentage \r
+ Identity</a></em><strong>.</strong></li>\r
+ <li><strong>Modify Identity Threshold<br>\r
+ </strong><em>Use this to set the threshold value for colouring above Identity. \r
+ Useful if the window has been closed. <br>\r
+ </em></li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Calculate</strong> \r
+ <ul>\r
+ <li>Sort \r
+ <ul>\r
+ <li><strong>by ID</strong><em><br>\r
+ This will sort the sequences according to sequence name. If the sort \r
+ is repeated, the order of the sorted sequences will be inverted. </em></li>\r
+ <li><strong>by Group</strong><strong><br>\r
+ </strong><em>This will sort the sequences according to sequence name. \r
+ If the sort is repeated, the order of the sorted sequences will be \r
+ inverted. </em><strong></strong></li>\r
+ <li><strong>by Pairwise Identity<br>\r
+ </strong><em>This will sort the selected sequences by their percentage \r
+ identity to the consensus sequence. The most similar sequence is put \r
+ at the top. </em></li>\r
+ <li><em>The <a href="../calculations/sorting.html">Sort menu</a> will \r
+ have some additional options if you have just done a multiple alignment \r
+ calculation, or opened a tree viewer window.</em></li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Calculate Tree </strong> <br>\r
+ <em>Functions for calculating trees on the alignment or the currently \r
+ selected region. See <a\r
+ href="../calculations/tree.html">calculating trees</a>.</em> \r
+ <ul>\r
+ <li><strong>Average Distance Using % Identity</strong></li>\r
+ <li><strong>Neighbour Joining Using % Identity</strong></li>\r
+ <li><strong>Average Distance Using Blosum62</strong></li>\r
+ <li><strong>Neighbour Joining Using Blosum62</strong></li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Pairwise Alignments</strong><br>\r
+ <em>Applies Smith and Waterman algorithm to selected sequences. See <a href="../calculations/pairwise.html">pairwise \r
+ alignments</a>.</em></li>\r
+ <li><strong>Principal Component Analysis</strong><br>\r
+ <em>Shows a spatial clustering of the sequences based on the BLOSUM62 \r
+ scores in the alignment. See <a href="../calculations/pca.html">Principal \r
+ Component Analysis</a>.</em> </li>\r
+ <li><strong>Translate cDNA</strong><br>\r
+ <em>If you are viewing a cDNA alignment a very simple translation service \r
+ is available. The translation ignores all gaps in the cDNA sequences. \r
+ </em> <br>\r
+ </li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Web Service<br>\r
+ </strong> <em>Selecting one of the following menu items starts a remote service \r
+ on compute facilities at the University of Dundee. You need a continuous network \r
+ connection in order to use these services through Jalview. </em> \r
+ <ul>\r
+ <li><strong>Alignment </strong> \r
+ <ul>\r
+ <li><strong>ClustalW Multiple Sequence Alignment</strong><br>\r
+ <em> Submits all, or just the currently selected sequences for alignment \r
+ with clustal W.</em></li>\r
+ <li><strong>ClustalW Multiple Sequence Alignment Realign</strong><br>\r
+ <em> Submits the alignment or currently selected region for re-alignment \r
+ with clustal W. Use this if you have added some new sequences to an \r
+ existing alignment.</em></li>\r
+ <li><strong>Muscle Multiple Protein Sequence Alignment</strong><br>\r
+ <em> Submits all, or jut the currently selected sequences for alignment \r
+ using Muscle. Do not use this if you are working with nucleic acid \r
+ sequences.</em></li>\r
+ </ul>\r
+ </li>\r
+ <li><strong>Secondary Structure Prediction</strong> \r
+ <ul>\r
+ <li><strong>JPred Secondary Structure Prediction</strong><br>\r
+ <em>Secondary structure prediction by network consensus. The behaviour \r
+ of this calculation depends on the current selection: </em></li>\r
+ <li><em>If nothing is selected, and the displayed sequences appear to \r
+ be aligned, then a JNet prediction will be run for the first sequence \r
+ in the alignment, using the current alignment. Otherwise the first \r
+ sequence will be submitted for prediction. </em></li>\r
+ <li><em>If just one sequence (or a region on one sequence) has been \r
+ selected, it will be submitted to the automatic JNet prediction server \r
+ for homolog detection and prediction. </em></li>\r
+ <li><em>If a set of sequences are selected, and they appear to be aligned, \r
+ then the alignment will be used for a Jnet prediction on the <strong>first</strong> \r
+ sequence selected in the set (that is, the one that was first clicked \r
+ on). </em> </li>\r
+ </ul>\r
+ </li>\r
+ </ul>\r
+ </li>\r