# are to be used:
# the substring between the first two double underscores is a
# unique identifier and needs to match the identifiers
-# in '% <parameter-type> <unique-id>=<value>' statements
+# in '% <parameter-type> <unique-id>=<value>' statements
# Example:
# alignment name : 'x__bcl2__e1'
-# parameter statments: '% RSL bcl2=60'
+# parameter statments: '% RSL bcl2=60'
$ PROBCONS=/home/czmasek/SOFTWARE/PROBCONS/probcons_v1_12/probcons
$ DIALIGN_TX=/home/czmasek/SOFTWARE/DIALIGNTX/DIALIGN-TX_1.0.2/source/dialign-tx
$ DIALIGN_CONF=/home/czmasek/SOFTWARE/DIALIGNTX/DIALIGN-TX_1.0.2/conf
% RSL Hormone_recep=60
-%
+%
% RSL Y_phosphatase=100
% RSL Y_phosphatase2=75
% RSL Y_phosphatase3=50
> KALIGN $ > $_kalign
-> MSA_PRO -o=p -n=10 -rr=0.5 -c -rsl=%[RSL]% $_kalign $_kalign_05_%[RSL]%.aln
+> MSA_PRO -o=p -n=10 -d -rr=0.5 -c -rsl=%[RSL]% $_kalign $_kalign_05_%[RSL]%.aln
> PHYLO_PL %[PHYLO_OPT]% $_kalign_05_%[RSL]%.aln $_kalign_05_%[RSL]% %[TMP_DIR]%
--
+-
+
+> KALIGN $ > $_kalign_
+> MSA_PRO -o=p -n=10 -d -rr=0.9 -c -rsl=%[RSL]% $_kalign_ $_kalign_09_%[RSL]%.aln
+> PHYLO_PL %[PHYLO_OPT]% $_kalign_09_%[RSL]%.aln $_kalign_09_%[RSL]% %[TMP_DIR]%
+-
+
> HMMALIGN --amino --trim --outformat Pfam -o $_hmmalign %[HMM]% $ > /dev/null
-> MSA_PRO -o=p -n=10 -rr=0.5 -c -rsl=%[RSL]% $_hmmalign $_hmmalign_05_%[RSL]%.aln
+> MSA_PRO -o=p -n=10 -d -rr=0.5 -c -rsl=%[RSL]% $_hmmalign $_hmmalign_05_%[RSL]%.aln
> PHYLO_PL %[PHYLO_OPT]% $_hmmalign_05_%[RSL]%.aln $_hmmalign_05_%[RSL]% %[TMP_DIR]%
--
+-
+
+> HMMALIGN --amino --trim --outformat Pfam -o $_hmmalign_ %[HMM]% $ > /dev/null
+> MSA_PRO -o=p -n=10 -d -rr=0.9 -c -rsl=%[RSL]% $_hmmalign_ $_hmmalign_09_%[RSL]%.aln
+> PHYLO_PL %[PHYLO_OPT]% $_hmmalign_09_%[RSL]%.aln $_hmmalign_09_%[RSL]% %[TMP_DIR]%
+-
+
> MAFFT --maxiterate 1000 --localpair $ > $_mafft
-> MSA_PRO -o=p -n=10 -rr=0.5 -c -rsl=%[RSL]% $_mafft $_mafft_05_%[RSL]%.aln
+> MSA_PRO -o=p -n=10 -d -rr=0.5 -c -rsl=%[RSL]% $_mafft $_mafft_05_%[RSL]%.aln
> PHYLO_PL %[PHYLO_OPT]% $_mafft_05_%[RSL]%.aln $_mafft_05_%[RSL]% %[TMP_DIR]%
-
+> MAFFT --maxiterate 1000 --localpair $ > $_mafft_
+> MSA_PRO -o=p -n=10 -d -rr=0.9 -c -rsl=%[RSL]% $_mafft_ $_mafft_09_%[RSL]%.aln
+> PHYLO_PL %[PHYLO_OPT]% $_mafft_09_%[RSL]%.aln $_mafft_09_%[RSL]% %[TMP_DIR]%
+-
+
+
> MUSCLE -maxiters 1000 -maxtrees 100 -in $ -out $_muscle
-> MSA_PRO -o=p -n=10 -rr=0.5 -c -rsl=%[RSL]% $_muscle $_muscle_05_%[RSL]%.aln
-> PHYLO_PL %[PHYLO_OPT]% $_muscle_05_%[RSL]%.aln $_muscle_05_%[RSL]% %[TMP_DIR]%
+> MSA_PRO -o=p -n=10 -d -rr=0.5 -c -rsl=%[RSL]% $_muscle $_muscle_05_%[RSL]%.aln
+> PHYLO_PL %[PHYLO_OPT]% $_muscle_05_%[RSL]%.aln $_muscle_05_%[RSL]% %[TMP_DIR]%
+-
+
+> MUSCLE -maxiters 1000 -maxtrees 100 -in $ -out $_muscle_
+> MSA_PRO -o=p -n=10 -d -rr=0.9 -c -rsl=%[RSL]% $_muscle_ $_muscle_09_%[RSL]%.aln
+> PHYLO_PL %[PHYLO_OPT]% $_muscle_09_%[RSL]%.aln $_muscle_09_%[RSL]% %[TMP_DIR]%
-
+
> CLUSTALO --full --full-iter --iter=5 -i $ -o $_clustalo
-> MSA_PRO -o=p -n=10 -rr=0.5 -c -rsl=%[RSL]% $_clustalo $_clustalo_05_%[RSL]%.aln
+> MSA_PRO -o=p -n=10 -d -rr=0.5 -c -rsl=%[RSL]% $_clustalo $_clustalo_05_%[RSL]%.aln
> PHYLO_PL %[PHYLO_OPT]% $_clustalo_05_%[RSL]%.aln $_clustalo_05_%[RSL]% %[TMP_DIR]%
-
+> CLUSTALO --full --full-iter --iter=5 -i $ -o $_clustalo_
+> MSA_PRO -o=p -n=10 -d -rr=0.9 -c -rsl=%[RSL]% $_clustalo_ $_clustalo_09_%[RSL]%.aln
+> PHYLO_PL %[PHYLO_OPT]% $_clustalo_09_%[RSL]%.aln $_clustalo_09_%[RSL]% %[TMP_DIR]%
+-
+
+
> PROBCONS $ > $_probcons
-> MSA_PRO -o=p -n=10 -rem_red -rr=0.5 -c -rsl=%[RSL]% $_probcons $_probcons_05_%[RSL]%.aln
+> MSA_PRO -o=p -n=10 -d -rem_red -rr=0.5 -c -rsl=%[RSL]% $_probcons $_probcons_05_%[RSL]%.aln
> PHYLO_PL %[PHYLO_OPT]% $_probcons_05_%[RSL]%.aln $_probcons_05_%[RSL]% %[TMP_DIR]%
--
+-
+
+> PROBCONS $ > $_probcons_
+> MSA_PRO -o=p -n=10 -d -rem_red -rr=0.9 -c -rsl=%[RSL]% $_probcons_ $_probcons_09_%[RSL]%.aln
+> PHYLO_PL %[PHYLO_OPT]% $_probcons_09_%[RSL]%.aln $_probcons_09_%[RSL]% %[TMP_DIR]%
+-
+
> DIALIGN_TX DIALIGN_CONF $ $_dialigntx
-> MSA_PRO -o=p -n=10 -rem_red -rr=0.5 -c -rsl=%[RSL]% $_dialigntx $_dialigntx_05_%[RSL]%.aln
+> MSA_PRO -o=p -n=10 -d -rem_red -rr=0.5 -c -rsl=%[RSL]% $_dialigntx $_dialigntx_05_%[RSL]%.aln
> PHYLO_PL %[PHYLO_OPT]% $_dialigntx_05_%[RSL]%.aln $_dialigntx_05_%[RSL]% %[TMP_DIR]%
-
+> DIALIGN_TX DIALIGN_CONF $ $_dialigntx_
+> MSA_PRO -o=p -n=10 -d -rem_red -rr=0.9 -c -rsl=%[RSL]% $_dialigntx_ $_dialigntx_09_%[RSL]%.aln
+> PHYLO_PL %[PHYLO_OPT]% $_dialigntx_09_%[RSL]%.aln $_dialigntx_09_%[RSL]% %[TMP_DIR]%
+-
+
=end