import jalview.datamodel.Sequence;
import jalview.datamodel.SequenceFeature;
import jalview.datamodel.SequenceI;
-import jalview.io.gff.SequenceOntology;
-import jalview.schemes.ResidueProperties;
import jalview.util.DBRefUtils;
-import jalview.util.MapList;
-import jalview.util.MappingUtils;
-import jalview.util.StringUtils;
import jalview.ws.SequenceFetcher;
import jalview.ws.seqfetcher.ASequenceFetcher;
import java.util.ArrayList;
-import java.util.Collections;
-import java.util.LinkedHashMap;
import java.util.List;
-import java.util.Map.Entry;
import java.util.Vector;
/**
*/
public class CrossRef
{
+ /*
+ * A sub-class that ignores Parent attribute when comparing sequence
+ * features. This avoids 'duplicate' CDS features that only
+ * differ in their parent Transcript ids.
+ */
+ class MySequenceFeature extends SequenceFeature
+ {
+ private SequenceFeature feat;
+
+ MySequenceFeature(SequenceFeature sf)
+ {
+ this.feat = sf;
+ }
+
+ @Override
+ public boolean equals(Object o)
+ {
+ return feat.equals(o, true);
+ }
+ }
+
/**
* Select just the DNA or protein references for a protein or dna sequence
*
{
dss = dss.getDatasetSequence();
}
- DBRefEntry[] rfs = findXDbRefs(dna, dss.getDBRef());
+ DBRefEntry[] rfs = findXDbRefs(dna, dss.getDBRefs());
if (rfs != null)
{
for (DBRefEntry ref : rfs)
if (dataset != null)
{
// search for references to this sequence's direct references.
- DBRefEntry[] lrfs = CrossRef.findXDbRefs(!dna, seq.getDBRef());
+ DBRefEntry[] lrfs = CrossRef.findXDbRefs(!dna, seq.getDBRefs());
List<SequenceI> rseqs = new ArrayList<SequenceI>();
CrossRef.searchDatasetXrefs(seq, !dna, lrfs, dataset, rseqs,
null); // don't need to specify codon frame for mapping here
for (SequenceI rs : rseqs)
{
- DBRefEntry[] xrs = findXDbRefs(dna, rs.getDBRef());
+ DBRefEntry[] xrs = findXDbRefs(dna, rs.getDBRefs());
if (xrs != null)
{
for (DBRefEntry ref : xrs)
Vector cseqs = new Vector();
for (int s = 0; s < seqs.length; s++)
{
- DBRefEntry[] cdna = findXDbRefs(true, seqs[s].getDBRef());
+ DBRefEntry[] cdna = findXDbRefs(true, seqs[s].getDBRefs());
for (int c = 0; c < cdna.length; c++)
{
if (cdna[c].getSource().equals(DBRefSource.EMBLCDS))
/**
*
- * @param dna
- * @param seqs
- * @return
- */
- public static Alignment findXrefSequences(SequenceI[] seqs, boolean dna,
- String source)
- {
- return findXrefSequences(seqs, dna, source, null);
- }
-
- /**
- *
* @param seqs
* sequences whose xrefs are being retrieved
* @param dna
* true if sequences are nucleotide
* @param source
- * @param dataset
- * alignment to search for product sequences.
+ * @param al
+ * alignment to search for cross-referenced sequences (and possibly
+ * add to)
+ * @param addedPeers
+ * a list of sequences to add to if 'peers' to the original sequences
+ * are found e.g. alternative protein products for a protein's gene
* @return products (as dataset sequences)
*/
public static Alignment findXrefSequences(SequenceI[] seqs, boolean dna,
- String source, AlignmentI dataset)
+ String source, AlignmentI al, List<SequenceI> addedPeers)
{
+ AlignmentI dataset = al.getDataset() == null ? al : al.getDataset();
List<SequenceI> rseqs = new ArrayList<SequenceI>();
AlignedCodonFrame cf = new AlignedCodonFrame();
for (SequenceI seq : seqs)
dss = dss.getDatasetSequence();
}
boolean found = false;
- DBRefEntry[] xrfs = CrossRef.findXDbRefs(dna, dss.getDBRef());
+ DBRefEntry[] xrfs = CrossRef.findXDbRefs(dna, dss.getDBRefs());
if ((xrfs == null || xrfs.length == 0) && dataset != null)
{
System.out.println("Attempting to find ds Xrefs refs.");
// FIXME should be dss not seq here?
- DBRefEntry[] lrfs = CrossRef.findXDbRefs(!dna, seq.getDBRef());
+ DBRefEntry[] lrfs = CrossRef.findXDbRefs(!dna, seq.getDBRefs());
// less ambiguous would be a 'find primary dbRefEntry' method.
// filter for desired source xref here
found = CrossRef.searchDatasetXrefs(dss, !dna, lrfs, dataset,
// map should be from protein seq to its coding dna
cf.addMap(rsq, dss, xref.getMap().getMap().getInverse());
}
-
- /*
- * compute peptide variants from dna variants
- */
- rsq.createDatasetSequence();
- computeProteinVariants(seq, rsq, xref.getMap().getMap());
}
found = true;
}
+ seq.getName());
e.printStackTrace();
}
+
if (retrieved != null)
{
+ List<SequenceFeature> copiedFeatures = new ArrayList<SequenceFeature>();
+ CrossRef me = new CrossRef();
for (int rs = 0; rs < retrieved.length; rs++)
{
// TODO: examine each sequence for 'redundancy'
- DBRefEntry[] dbr = retrieved[rs].getDBRef();
+ DBRefEntry[] dbr = retrieved[rs].getDBRefs();
if (dbr != null && dbr.length > 0)
{
for (int di = 0; di < dbr.length; di++)
int sf = map.getMap().getToLowest();
int st = map.getMap().getToHighest();
SequenceI mappedrg = ms.getSubSequence(sf, st);
- SequenceI loc = dss.getSubSequence(sf, st);
+ // SequenceI loc = dss.getSubSequence(sf, st);
if (mappedrg.getLength() > 0
- && mappedrg.getSequenceAsString().equals(
- loc.getSequenceAsString()))
+ && ms.getSequenceAsString().equals(
+ dss.getSequenceAsString()))
+ // && mappedrg.getSequenceAsString().equals(
+ // loc.getSequenceAsString()))
{
- System.err
- .println("Mapping updated for retrieved crossreference");
+ String msg = "Mapping updated from "
+ + ms.getName()
+ + " to retrieved crossreference "
+ + dss.getName();
+ System.out.println(msg);
// method to update all refs of existing To on
// retrieved sequence with dss and merge any props
// on To onto dss.
map.setTo(dss);
+ /*
+ * copy sequence features as well, avoiding
+ * duplication (e.g. from 2 transcripts)
+ */
+ SequenceFeature[] sfs = ms
+ .getSequenceFeatures();
+ if (sfs != null)
+ {
+ for (SequenceFeature feat : sfs)
+ {
+ /*
+ * we override SequenceFeature.equals here (but
+ * not elsewhere) to ignore Parent attribute
+ * TODO not quite working yet!
+ */
+ if (!copiedFeatures
+ .contains(me.new MySequenceFeature(
+ feat)))
+ {
+ dss.addSequenceFeature(feat);
+ copiedFeatures.add(feat);
+ }
+ }
+ }
+ cf.addMap(retrieved[rs].getDatasetSequence(),
+ dss, map.getMap());
+ }
+ else
+ {
+ addedPeers.add(map.getTo());
+ cf.addMap(retrieved[rs].getDatasetSequence(),
+ map.getTo(), map.getMap());
}
} catch (Exception e)
{
Alignment ral = null;
if (rseqs.size() > 0)
{
- SequenceI[] rsqs = new SequenceI[rseqs.size()];
- rseqs.toArray(rsqs);
- ral = new Alignment(rsqs);
+ ral = new Alignment(rseqs.toArray(new SequenceI[rseqs.size()]));
if (cf != null && !cf.isEmpty())
{
ral.addCodonFrame(cf);
}
// look for direct or indirect references in common
- DBRefEntry[] poss = nxt.getDBRef(), cands = null;
+ DBRefEntry[] poss = nxt.getDBRefs(), cands = null;
if (direct)
{
cands = jalview.util.DBRefUtils.searchRefs(poss, xrf);
}
/**
- * Computes variants in peptide product generated by variants in dna, and adds
- * them as sequence_variant features on the protein sequence. Returns the
- * number of variant features added.
- *
- * @param dnaSeq
- * @param peptide
- * @param dnaToProtein
- */
- protected static int computeProteinVariants(SequenceI dnaSeq,
- SequenceI peptide, MapList dnaToProtein)
- {
- /*
- * map from peptide position to all variant features of the codon for it
- * LinkedHashMap ensures we add the peptide features in sequence order
- */
- LinkedHashMap<Integer, String[][]> variants = new LinkedHashMap<Integer, String[][]>();
- SequenceOntology so = SequenceOntology.getInstance();
-
- SequenceFeature[] dnaFeatures = dnaSeq.getSequenceFeatures();
- if (dnaFeatures == null)
- {
- return 0;
- }
-
- int[] lastCodon = null;
- int lastPeptidePostion = 0;
-
- /*
- * build a map of codon variations for peptides
- */
- for (SequenceFeature sf : dnaFeatures)
- {
- int dnaCol = sf.getBegin();
- if (dnaCol != sf.getEnd())
- {
- // not handling multi-locus variant features
- continue;
- }
- if (so.isSequenceVariant(sf.getType()))
- {
- int[] mapsTo = dnaToProtein.locateInTo(dnaCol, dnaCol);
- if (mapsTo == null)
- {
- // feature doesn't lie within coding region
- continue;
- }
- int peptidePosition = mapsTo[0];
- String[][] codonVariants = variants.get(peptidePosition);
- if (codonVariants == null)
- {
- codonVariants = new String[3][];
- variants.put(peptidePosition, codonVariants);
- }
-
- /*
- * extract dna variants to a string array
- */
- String alls = (String) sf.getValue("alleles");
- if (alls == null)
- {
- continue;
- }
- String[] alleles = alls.split(",");
-
- /*
- * get this peptides codon positions e.g. [3, 4, 5] or [4, 7, 10]
- */
- int[] codon = peptidePosition == lastPeptidePostion ? lastCodon
- : MappingUtils.flattenRanges(dnaToProtein.locateInFrom(
- peptidePosition, peptidePosition));
- lastPeptidePostion = peptidePosition;
- lastCodon = codon;
-
- /*
- * save nucleotide (and this variant) for each codon position
- */
- for (int codonPos = 0; codonPos < 3; codonPos++)
- {
- String nucleotide = String.valueOf(dnaSeq
- .getCharAt(codon[codonPos] - 1));
- if (codon[codonPos] == dnaCol)
- {
- /*
- * record current dna base and its alleles
- */
- String[] dnaVariants = new String[alleles.length + 1];
- dnaVariants[0] = nucleotide;
- System.arraycopy(alleles, 0, dnaVariants, 1, alleles.length);
- codonVariants[codonPos] = dnaVariants;
- }
- else if (codonVariants[codonPos] == null)
- {
- /*
- * record current dna base only
- * (at least until we find any variation and overwrite it)
- */
- codonVariants[codonPos] = new String[] { nucleotide };
- }
- }
- }
- }
-
- /*
- * scan codon variations, compute peptide variants and add to peptide sequence
- */
- int count = 0;
- for (Entry<Integer, String[][]> variant : variants.entrySet())
- {
- int peptidePos = variant.getKey();
- String[][] codonVariants = variant.getValue();
- String residue = String.valueOf(peptide.getCharAt(peptidePos - 1)); // 0-based
- List<String> peptideVariants = computePeptideVariants(codonVariants,
- residue);
- if (!peptideVariants.isEmpty())
- {
- Collections.sort(peptideVariants);
- String desc = StringUtils.listToDelimitedString(peptideVariants,
- ", ");
- SequenceFeature sf = new SequenceFeature(
- SequenceOntology.SEQUENCE_VARIANT, desc, peptidePos,
- peptidePos, Float.NaN, null);
- peptide.getDatasetSequence().addSequenceFeature(sf);
- count++;
- }
- }
- return count;
- }
-
- /**
- * Returns a non-redundant list of all peptide translations generated by the
- * given dna variants, excluding the current residue value
- *
- * @param codonVariants
- * an array of base values for codon positions 1, 2, 3
- * @param residue
- * the current residue translation
- * @return
- */
- protected static List<String> computePeptideVariants(
- String[][] codonVariants, String residue)
- {
- List<String> result = new ArrayList<String>();
- for (String base1 : codonVariants[0])
- {
- for (String base2 : codonVariants[1])
- {
- for (String base3 : codonVariants[2])
- {
- String codon = base1 + base2 + base3;
- // TODO: report frameshift/insertion/deletion
- // and multiple-base variants?!
- String peptide = codon.contains("-") ? "-" : ResidueProperties
- .codonTranslate(codon);
- if (peptide != null && !result.contains(peptide)
- && !peptide.equals(residue))
- {
- result.add(peptide);
- }
- }
- }
- }
- return result;
- }
-
- /**
- * Computes a list of all peptide variants given dna variants
- *
- * @param dnaSeq
- * the coding dna sequence
- * @param codonVariants
- * variant features for each codon position (null if no variant)
- * @param residue
- * the canonical protein translation
- * @return
- */
- protected static List<String> computePeptideVariants(SequenceI dnaSeq,
- SequenceFeature[] codonVariants, String residue)
- {
- List<String> result = new ArrayList<String>();
- int[][] dnaVariants = new int[3][];
- for (int i = 0; i < 3; i++)
- {
-
- }
- // TODO Auto-generated method stub
- return null;
- }
-
- /**
* precalculate different products that can be found for seqs in dataset and
* return them.
*
git://source.jalview.org / jalview.git / blobdiff