import jalview.io.gff.SequenceOntologyFactory;
import jalview.io.gff.SequenceOntologyI;
+import java.util.Arrays;
import java.util.List;
import com.stevesoft.pat.Regex;
+/**
+ * A client to fetch CDNA sequence from Ensembl (i.e. that part of the genomic
+ * sequence that is transcribed to RNA, but not necessarily translated to
+ * protein)
+ *
+ * @author gmcarstairs
+ *
+ */
public class EnsemblCdna extends EnsemblSeqProxy
{
+ private static final List<String> CROSS_REFERENCES = Arrays
+ .asList(new String[] { "Uniprot/SWISSPROT", "Uniprot/SPTREMBL" });
+
+ /*
+ * accepts ENST or ENSTG with 11 digits
+ * or ENSMUST or similar for other species
+ * or CCDSnnnnn.nn with at least 3 digits
+ */
+ private static final Regex ACCESSION_REGEX = new Regex(
+ "(ENS([A-Z]{3}|)[TG][0-9]{11}$)" + "|" + "(CCDS[0-9.]{3,}$)");
+
/*
- * fetch exon features on genomic sequence (to identify the cdnaregions)
+ * fetch exon features on genomic sequence (to identify the cdna regions)
* and cds and variation features (to retain)
*/
private static final EnsemblFeatureType[] FEATURES_TO_FETCH = {
EnsemblFeatureType.exon, EnsemblFeatureType.cds,
EnsemblFeatureType.variation };
+ /**
+ * Default constructor (to use rest.ensembl.org)
+ */
public EnsemblCdna()
{
super();
}
+ /**
+ * Constructor given the target domain to fetch data from
+ *
+ * @param d
+ */
+ public EnsemblCdna(String d)
+ {
+ super(d);
+ }
+
@Override
public String getDbName()
{
@Override
public Regex getAccessionValidator()
{
- return new Regex("((ENST|ENSG|CCDS)[0-9.]{3,})");
+ return ACCESSION_REGEX;
}
@Override
@Override
protected List<String> getCrossReferenceDatabases()
{
- return super.getCrossReferenceDatabases();
+ return CROSS_REFERENCES;
// 30/01/16 also found Vega_transcript, OTTT, ENS_LRG_transcript, UCSC,
// HGNC_trans_name, RefSeq_mRNA, RefSeq_mRNA_predicted
}