JAL-4389 Adjust preferences menu to work with any WSDiscoverer
[jalview.git] / src / jalview / gui / Preferences.java
index 827c9a0..67b45e3 100755 (executable)
@@ -27,16 +27,21 @@ import java.awt.Dimension;
 import java.awt.Font;
 import java.awt.event.ActionEvent;
 import java.awt.event.ActionListener;
+import java.awt.event.FocusAdapter;
+import java.awt.event.FocusEvent;
 import java.awt.event.MouseEvent;
 import java.io.File;
 import java.util.ArrayList;
 import java.util.List;
+import java.util.concurrent.CompletableFuture;
 
 import javax.help.HelpSetException;
 import javax.swing.JComboBox;
 import javax.swing.JFileChooser;
 import javax.swing.JInternalFrame;
 import javax.swing.JPanel;
+import javax.swing.JTabbedPane;
+import javax.swing.JTextField;
 import javax.swing.ListSelectionModel;
 import javax.swing.RowFilter;
 import javax.swing.RowSorter;
@@ -50,9 +55,14 @@ import javax.swing.table.TableColumn;
 import javax.swing.table.TableModel;
 import javax.swing.table.TableRowSorter;
 
+import jalview.hmmer.HmmerCommand;
+import jalview.util.FileUtils;
 import ext.edu.ucsf.rbvi.strucviz2.StructureManager;
 import jalview.analysis.AnnotationSorter.SequenceAnnotationOrder;
+import jalview.bin.ApplicationSingletonProvider.ApplicationSingletonI;
 import jalview.bin.Cache;
+import jalview.bin.Console;
+import jalview.bin.MemorySetting;
 import jalview.ext.pymol.PymolManager;
 import jalview.gui.Help.HelpId;
 import jalview.gui.StructureViewer.ViewerType;
@@ -74,6 +84,10 @@ import jalview.util.MessageManager;
 import jalview.util.Platform;
 import jalview.util.UrlConstants;
 import jalview.ws.sifts.SiftsSettings;
+import jalview.ws2.client.api.WebServiceDiscovererI;
+import jalview.ws2.client.ebi.JobDispatcherWSDiscoverer;
+import jalview.ws2.client.jpred4.JPred4WSDiscoverer;
+import jalview.ws2.client.slivka.SlivkaWSDiscoverer;
 
 /**
  * DOCUMENT ME!
@@ -81,47 +95,154 @@ import jalview.ws.sifts.SiftsSettings;
  * @author $author$
  * @version $Revision$
  */
-public class Preferences extends GPreferences
+public class Preferences extends GPreferences implements ApplicationSingletonI
 {
-  public static final String ENABLE_SPLIT_FRAME = "ENABLE_SPLIT_FRAME";
+  // suggested list delimiter character
+  public static final String COMMA = ",";
 
-  public static final String SCALE_PROTEIN_TO_CDNA = "SCALE_PROTEIN_TO_CDNA";
+  public static final String HMMSEARCH_SEQCOUNT = "HMMSEARCH_SEQCOUNT";
 
-  public static final String DEFAULT_COLOUR = "DEFAULT_COLOUR";
+  public static final String HMMINFO_GLOBAL_BACKGROUND = "HMMINFO_GLOBAL_BACKGROUND";
 
-  public static final String DEFAULT_COLOUR_PROT = "DEFAULT_COLOUR_PROT";
-
-  public static final String DEFAULT_COLOUR_NUC = "DEFAULT_COLOUR_NUC";
+  public static final String HMMALIGN_TRIM_TERMINI = "HMMALIGN_TRIM_TERMINI";
+  
+  public static final String ADD_SS_ANN = "ADD_SS_ANN";
 
   public static final String ADD_TEMPFACT_ANN = "ADD_TEMPFACT_ANN";
 
-  public static final String ADD_SS_ANN = "ADD_SS_ANN";
+  public static final String ALLOW_UNPUBLISHED_PDB_QUERYING = "ALLOW_UNPUBLISHED_PDB_QUERYING";
 
-  public static final String USE_RNAVIEW = "USE_RNAVIEW";
+  public static final String ANNOTATIONCOLOUR_MAX = "ANNOTATIONCOLOUR_MAX";
 
-  public static final String STRUCT_FROM_PDB = "STRUCT_FROM_PDB";
+  public static final String ANNOTATIONCOLOUR_MIN = "ANNOTATIONCOLOUR_MIN";
 
-  public static final String STRUCTURE_DISPLAY = "STRUCTURE_DISPLAY";
+  public static final String ANTI_ALIAS = "ANTI_ALIAS";
+
+  public static final String AUTO_CALC_CONSENSUS = "AUTO_CALC_CONSENSUS";
+
+  public static final String AUTOASSOCIATE_PDBANDSEQS = "AUTOASSOCIATE_PDBANDSEQS";
+
+  public static final String BLOSUM62_PCA_FOR_NUCLEOTIDE = "BLOSUM62_PCA_FOR_NUCLEOTIDE";
+
+  public static final String CENTRE_COLUMN_LABELS = "CENTRE_COLUMN_LABELS";
 
   public static final String CHIMERA_PATH = "CHIMERA_PATH";
 
   public static final String CHIMERAX_PATH = "CHIMERAX_PATH";
 
+  public static final String DBREFFETCH_USEPICR = "DBREFFETCH_USEPICR";
+
+  public static final String DEFAULT_COLOUR = "DEFAULT_COLOUR";
+
+  public static final String DEFAULT_COLOUR_NUC = "DEFAULT_COLOUR_NUC";
+  public static final String DEFAULT_COLOUR_PROT = "DEFAULT_COLOUR_PROT";
+
+  public static final String ENABLE_SPLIT_FRAME = "ENABLE_SPLIT_FRAME";
+
+  public static final String FIGURE_AUTOIDWIDTH = "FIGURE_AUTOIDWIDTH";
+
+  public static final String FIGURE_FIXEDIDWIDTH = "FIGURE_FIXEDIDWIDTH";
+
+  public static final String FOLLOW_SELECTIONS = "FOLLOW_SELECTIONS";
+
+  public static final String FONT_NAME = "FONT_NAME";
+
+  public static final String FONT_SIZE = "FONT_SIZE";
+
+  public static final String FONT_STYLE = "FONT_STYLE";
+  
+  public static final String HMMER_PATH = "HMMER_PATH";
+
+  public static final String CYGWIN_PATH = "CYGWIN_PATH";
+
+  public static final String HMMSEARCH_DBS = "HMMSEARCH_DBS";
+
+  public static final String GAP_COLOUR = "GAP_COLOUR";
+
+  public static final String GAP_SYMBOL = "GAP_SYMBOL";
+
+  public static final String HIDDEN_COLOUR = "HIDDEN_COLOUR";
+
+  public static final String HIDE_INTRONS = "HIDE_INTRONS";
+
+  public static final String ID_ITALICS = "ID_ITALICS";
+
+  public static final String ID_ORG_HOSTURL = "ID_ORG_HOSTURL";
+
+  public static final String MAP_WITH_SIFTS = "MAP_WITH_SIFTS";
+
+  public static final String NOQUESTIONNAIRES = "NOQUESTIONNAIRES";
+
+  public static final String NORMALISE_CONSENSUS_LOGO = "NORMALISE_CONSENSUS_LOGO";
+
+  public static final String NORMALISE_LOGO = "NORMALISE_LOGO";
+
+  public static final String PAD_GAPS = "PAD_GAPS";
+
+  public static final String PDB_DOWNLOAD_FORMAT = "PDB_DOWNLOAD_FORMAT";
   public static final String PYMOL_PATH = "PYMOL_PATH";
 
-  public static final String SORT_ANNOTATIONS = "SORT_ANNOTATIONS";
+  public static final String QUESTIONNAIRE = "QUESTIONNAIRE";
+
+  public static final String RELAXEDSEQIDMATCHING = "RELAXEDSEQIDMATCHING";
+
+  public static final String RIGHT_ALIGN_IDS = "RIGHT_ALIGN_IDS";
+
+  public static final String SCALE_PROTEIN_TO_CDNA = "SCALE_PROTEIN_TO_CDNA";
+
+  public static final String SHOW_ANNOTATIONS = "SHOW_ANNOTATIONS";
 
   public static final String SHOW_AUTOCALC_ABOVE = "SHOW_AUTOCALC_ABOVE";
 
+  public static final String SHOW_CONSENSUS = "SHOW_CONSENSUS";
+
+  public static final String SHOW_CONSENSUS_HISTOGRAM = "SHOW_CONSENSUS_HISTOGRAM";
+
+  public static final String SHOW_CONSENSUS_LOGO = "SHOW_CONSENSUS_LOGO";
+
+  public static final String SHOW_CONSERVATION = "SHOW_CONSERVATION";
+
+  public static final String SHOW_DBREFS_TOOLTIP = "SHOW_DBREFS_TOOLTIP";
+
+  public static final String SHOW_GROUP_CONSENSUS = "SHOW_GROUP_CONSENSUS";
+
+  public static final String SHOW_GROUP_CONSERVATION = "SHOW_GROUP_CONSERVATION";
+
+  public static final String SHOW_JVSUFFIX = "SHOW_JVSUFFIX";
+
+  public static final String SHOW_NPFEATS_TOOLTIP = "SHOW_NPFEATS_TOOLTIP";
   public static final String SHOW_OCCUPANCY = "SHOW_OCCUPANCY";
 
   public static final String SHOW_OV_HIDDEN_AT_START = "SHOW_OV_HIDDEN_AT_START";
 
+  public static final String SHOW_OVERVIEW = "SHOW_OVERVIEW";
+
+  public static final String SHOW_QUALITY = "SHOW_QUALITY";
+
+  public static final String SHOW_UNCONSERVED = "SHOW_UNCONSERVED";
+
+  public static final String SORT_ALIGNMENT = "SORT_ALIGNMENT";
+
+  public static final String SORT_ANNOTATIONS = "SORT_ANNOTATIONS";
+
+  public static final String SORT_BY_TREE = "SORT_BY_TREE";
+
+  public static final String STRUCT_FROM_PDB = "STRUCT_FROM_PDB";
+
+  public static final String STRUCTURE_DISPLAY = "STRUCTURE_DISPLAY";
+
+  public static final String STRUCTURE_DIMENSIONS = "STRUCTURE_DIMENSIONS";
+
+  public static final String UNIPROT_DOMAIN = "UNIPROT_DOMAIN";
+
+  public static final String USE_FULL_SO = "USE_FULL_SO";
   public static final String USE_LEGACY_GAP = "USE_LEGACY_GAP";
 
-  public static final String GAP_COLOUR = "GAP_COLOUR";
+  public static final String USE_RNAVIEW = "USE_RNAVIEW";
 
-  public static final String HIDDEN_COLOUR = "HIDDEN_COLOUR";
+  public static final String USER_DEFINED_COLOURS = "USER_DEFINED_COLOURS";
+
+  public static final String WRAP_ALIGNMENT = "WRAP_ALIGNMENT";
 
   private static final int MIN_FONT_SIZE = 1;
 
@@ -129,6 +250,8 @@ public class Preferences extends GPreferences
 
   private String previousProxyType;
 
+  private static Preferences INSTANCE = null; // add "final"
+
   /**
    * Holds name and link separated with | character. Sequence ID must be
    * $SEQUENCE_ID$ or $SEQUENCE_ID=/.possible | chars ./=$
@@ -139,10 +262,10 @@ public class Preferences extends GPreferences
 
   /**
    * Holds name and link separated with | character. Sequence IDS and Sequences
-   * must be $SEQUENCEIDS$ or $SEQUENCEIDS=/.possible | chars ./=$ and
-   * $SEQUENCES$ or $SEQUENCES=/.possible | chars ./=$ and separation character
-   * for first and second token specified after a pipe character at end |,|.
-   * (TODO: proper escape for using | to separate ids or sequences
+   * must be $SEQUENCEIDS$ or $SEQUENCEIDS=/.possible | chars ./=$ and $SEQUENCES$
+   * or $SEQUENCES=/.possible | chars ./=$ and separation character for first and
+   * second token specified after a pipe character at end |,|. (TODO: proper
+   * escape for using | to separate ids or sequences
    */
 
   public static List<String> groupURLLinks;
@@ -188,10 +311,56 @@ public class Preferences extends GPreferences
   private OptionsParam textOpt = new OptionsParam(
           MessageManager.getString("action.text"), "Text");
 
+  // get singleton Preferences instance
+  public static Preferences getInstance()
+  {
+    if (INSTANCE == null || INSTANCE.frame == null
+            || INSTANCE.frame.isClosed())
+    {
+      INSTANCE = new Preferences();
+    }
+    return INSTANCE;
+
+    /*
+     * Replace code with the following for Jalvew-JS
+    Preferences INSTANCE = ApplicationSingletonProvider.getInstance(Preferences.class);
+    if (INSTANCE == null || INSTANCE.frame == null
+            || INSTANCE.frame.isClosed())
+    {
+      ApplicationSingletonProvider.remove(Preferences.class);
+      INSTANCE = ApplicationSingletonProvider.getInstance(Preferences.class);
+    }
+    return INSTANCE;
+    */
+  }
+
+  public static void openPreferences()
+  {
+    openPreferences(null, null);
+  }
+
+  public static void openPreferences(TabRef selectTab, String message)
+  {
+    Preferences p = getInstance();
+    if (selectTab != null)
+      p.selectTab(selectTab, message);
+    p.frame.show();
+    p.frame.moveToFront();
+    p.frame.grabFocus();
+  }
+
+  public void selectTab(TabRef selectTab, String message)
+  {
+    this.selectTab(selectTab);
+    if (message != null)
+      this.setMessage(message);
+    this.frame.show();
+  }
+
   /**
    * Creates a new Preferences object.
    */
-  public Preferences()
+  private Preferences()
   {
     super();
     frame = new JInternalFrame();
@@ -206,6 +375,14 @@ public class Preferences extends GPreferences
       wsPrefs = new WsPreferences();
       wsTab.add(wsPrefs, BorderLayout.CENTER);
     }
+
+    if (Cache.getDefault("SHOW_SLIVKA_SERVICES", true))
+      addWebServiceTab(tabbedPane, SlivkaWSDiscoverer.getInstance());
+    if (Cache.getDefault("SHOW_EBI_SERVICES", true))
+      addWebServiceTab(tabbedPane, JobDispatcherWSDiscoverer.getInstance());
+    if (Cache.getDefault("SHOW_JPRED4_SERVICES", true))
+      addWebServiceTab(tabbedPane, JPred4WSDiscoverer.getInstance());
+
     int width = 500, height = 450;
     if (Platform.isAMacAndNotJS())
     {
@@ -218,6 +395,63 @@ public class Preferences extends GPreferences
     frame.setMinimumSize(new Dimension(width, height));
 
     /*
+     * Set HMMER tab defaults
+     */
+    hmmrTrimTermini.setSelected(Cache.getDefault(HMMALIGN_TRIM_TERMINI, false));
+    if (Cache.getDefault(HMMINFO_GLOBAL_BACKGROUND, false))
+    {
+      hmmerBackgroundUniprot.setSelected(true);
+    }
+    else
+    {
+      hmmerBackgroundAlignment.setSelected(true);
+    }
+    hmmerSequenceCount
+            .setText(Cache.getProperty(HMMSEARCH_SEQCOUNT));
+    hmmerPath.setText(Cache.getProperty(HMMER_PATH));
+    hmmerPath.addActionListener(new ActionListener()
+    {
+      @Override
+      public void actionPerformed(ActionEvent e)
+      {
+        validateHmmerPath();
+      }
+    });
+    hmmerPath.addFocusListener(new FocusAdapter()
+    {
+      @Override
+      public void focusLost(FocusEvent e)
+      {
+        validateHmmerPath();
+      }
+    });
+    if (cygwinPath != null)
+    {
+      String path = Cache.getProperty(CYGWIN_PATH);
+      if (path == null)
+      {
+        path = FileUtils.getPathTo("bash");
+      }
+      cygwinPath.setText(path);
+      cygwinPath.addActionListener(new ActionListener()
+      {
+        @Override
+        public void actionPerformed(ActionEvent e)
+        {
+          validateCygwinPath();
+        }
+      });
+      cygwinPath.addFocusListener(new FocusAdapter()
+      {
+        @Override
+        public void focusLost(FocusEvent e)
+        {
+          validateCygwinPath();
+        }
+      });
+    }
+
+    /*
      * Set Visual tab defaults
      */
     seqLimit.setSelected(Cache.getDefault("SHOW_JVSUFFIX", true));
@@ -240,6 +474,9 @@ public class Preferences extends GPreferences
             Cache.getDefault("SHOW_CONSENSUS_HISTOGRAM", true));
     showConsensLogo
             .setSelected(Cache.getDefault("SHOW_CONSENSUS_LOGO", false));
+    showInformationHistogram.setSelected(
+            Cache.getDefault("SHOW_INFORMATION_HISTOGRAM", true));
+    showHMMLogo.setSelected(Cache.getDefault("SHOW_HMM_LOGO", false));
     showNpTooltip
             .setSelected(Cache.getDefault("SHOW_NPFEATS_TOOLTIP", true));
     showDbRefTooltip
@@ -302,8 +539,8 @@ public class Preferences extends GPreferences
     startupCheckbox
             .setSelected(Cache.getDefault("SHOW_STARTUP_FILE", true));
     startupFileTextfield.setText(Cache.getDefault("STARTUP_FILE",
-            Cache.getDefault("www.jalview.org", "http://www.jalview.org")
-                    + "/examples/exampleFile_2_3.jar"));
+            Cache.getDefault("www.jalview.org", "https://www.jalview.org")
+                    + "/examples/exampleFile_2_7.jvp"));
 
     /*
      * Set Colours tab defaults
@@ -330,12 +567,10 @@ public class Preferences extends GPreferences
     /*
      * Set overview panel defaults
      */
-    gapColour.setBackground(
-            Cache.getDefaultColour(GAP_COLOUR,
-                    jalview.renderer.OverviewResColourFinder.OVERVIEW_DEFAULT_GAP));
-    hiddenColour.setBackground(
-            Cache.getDefaultColour(HIDDEN_COLOUR,
-                    jalview.renderer.OverviewResColourFinder.OVERVIEW_DEFAULT_HIDDEN));
+    gapColour.setBackground(Cache.getDefaultColour(GAP_COLOUR,
+            jalview.renderer.OverviewResColourFinder.OVERVIEW_DEFAULT_GAP));
+    hiddenColour.setBackground(Cache.getDefaultColour(HIDDEN_COLOUR,
+            jalview.renderer.OverviewResColourFinder.OVERVIEW_DEFAULT_HIDDEN));
     useLegacyGap.setSelected(Cache.getDefault(USE_LEGACY_GAP, false));
     gapLabel.setEnabled(!useLegacyGap.isSelected());
     gapColour.setEnabled(!useLegacyGap.isSelected());
@@ -345,20 +580,24 @@ public class Preferences extends GPreferences
     /*
      * Set Structure tab defaults
      */
-    final boolean structSelected = Cache.getDefault(STRUCT_FROM_PDB, false);
+    final boolean structSelected = Cache.getDefault(STRUCT_FROM_PDB, true);
     structFromPdb.setSelected(structSelected);
-    useRnaView.setSelected(Cache.getDefault(USE_RNAVIEW, false));
-    useRnaView.setEnabled(structSelected);
-    addSecondaryStructure.setSelected(Cache.getDefault(ADD_SS_ANN, false));
+    addSecondaryStructure.setSelected(Cache.getDefault(ADD_SS_ANN, true));
     addSecondaryStructure.setEnabled(structSelected);
-    addTempFactor.setSelected(Cache.getDefault(ADD_TEMPFACT_ANN, false));
+    addTempFactor.setSelected(Cache.getDefault(ADD_TEMPFACT_ANN, true));
     addTempFactor.setEnabled(structSelected);
 
     /*
      * set choice of structure viewer, and path if saved as a preference;
      * default to Jmol (first choice) if an unexpected value is found
      */
-    String viewerType = Cache.getDefault(STRUCTURE_DISPLAY, ViewerType.JMOL.name());
+    String viewerType = ViewerType.JMOL.name();
+    if (!Platform.isJS())
+    {
+      Cache.getDefault(STRUCTURE_DISPLAY,
+            ViewerType.JMOL.name());
+    }
+    // TODO - disable external viewers for JS
     structViewer.setSelectedItem(viewerType);
     String viewerPath = "";
     ViewerType type = null;
@@ -381,7 +620,7 @@ public class Preferences extends GPreferences
       }
     } catch (IllegalArgumentException e)
     {
-      Cache.log.error("Unknown structure viewer type: " + viewerType
+      Console.error("Unknown structure viewer type: " + viewerType
               + ", defaulting to Jmol");
       type = ViewerType.JMOL;
     }
@@ -394,10 +633,28 @@ public class Preferences extends GPreferences
       {
         if (validateViewerPath())
         {
-          Cache.setProperty(structViewer.getSelectedItem()
-                  .equals(ViewerType.CHIMERAX.name())
-                  ? CHIMERAX_PATH
-                  : CHIMERA_PATH, structureViewerPath.getText());
+          String path = structureViewerPath.getText();
+          try
+          {
+            ViewerType type = ViewerType.valueOf(viewerType);
+            switch (type)
+            {
+            case JMOL:
+              break;
+            case CHIMERA:
+              Cache.setProperty(CHIMERA_PATH, path);
+              break;
+            case CHIMERAX:
+              Cache.setProperty(CHIMERAX_PATH, path);
+              break;
+            case PYMOL:
+              Cache.setProperty(PYMOL_PATH, path);
+              break;
+            }
+          } catch (IllegalArgumentException x)
+          {
+            Console.error("Failed to set path - unknown viewer type", x);
+          }
         }
       }
     });
@@ -552,9 +809,9 @@ public class Preferences extends GPreferences
       proxyType.setSelected(customProxy.getModel(), true);
       break;
     default:
-      String message = "Incorrect PROXY_TYPE - should be 'none' (clear proxy properties), 'false' (system settings), 'true' (custom settings): "
-              + proxyTypeString;
-      Cache.log.warn(message);
+      Console.warn(
+              "Incorrect PROXY_TYPE - should be 'none' (clear proxy properties), 'false' (system settings), 'true' (custom settings): "
+                      + proxyTypeString);
     }
     proxyServerHttpTB.setText(Cache.getDefault("PROXY_SERVER", ""));
     proxyPortHttpTB.setText(Cache.getDefault("PROXY_PORT", ""));
@@ -563,9 +820,11 @@ public class Preferences extends GPreferences
     proxyAuth.setSelected(Cache.getDefault("PROXY_AUTH", false));
     proxyAuthUsernameTB
             .setText(Cache.getDefault("PROXY_AUTH_USERNAME", ""));
-    proxyAuthPasswordTB
-            .setText(Cache.getDefault("PROXY_AUTH_PASSWORD", ""));
+    // we are not storing or retrieving proxy password from .jalview_properties
+    proxyAuthPasswordPB.setText(Cache.proxyAuthPassword == null ? ""
+            : new String(Cache.proxyAuthPassword));
     setCustomProxyEnabled();
+    applyProxyButtonEnabled(false);
 
     defaultBrowser.setText(Cache.getDefault("DEFAULT_BROWSER", ""));
 
@@ -609,6 +868,7 @@ public class Preferences extends GPreferences
 
     annotations_actionPerformed(null); // update the display of the annotation
                                        // settings
+
     
     
     /*
@@ -617,6 +877,16 @@ public class Preferences extends GPreferences
     loadLastSavedBackupsOptions();
   }
 
+  
+  private void addWebServiceTab(JTabbedPane tabs, WebServiceDiscovererI discoverer)
+  {
+    String name = discoverer.getName();
+    JPanel tab = new JPanel();
+    tab.setLayout(new BorderLayout());
+    tab.add(new WS2PreferencesPanel(discoverer), BorderLayout.CENTER);
+    tabs.add(tab, name + " services");
+  }
+  
   /**
    * A helper method that sets the items and initial selection in a character
    * rendering option list (Prompt each time/Lineart/Text)
@@ -630,6 +900,7 @@ public class Preferences extends GPreferences
     comboBox.addItem(promptEachTimeOpt);
     comboBox.addItem(lineArtOpt);
     comboBox.addItem(textOpt);
+
     
     /*
      * JalviewJS doesn't support Lineart so force it to Text
@@ -664,68 +935,77 @@ public class Preferences extends GPreferences
       return;
     }
 
+    /* 
+     * Set proxy settings first (to be before web services refresh)
+     */
+    saveProxySettings();
+
     /*
      * Save Visual settings
      */
-    Cache.applicationProperties.setProperty("SHOW_JVSUFFIX",
+    Cache.setPropertyNoSave("SHOW_JVSUFFIX",
             Boolean.toString(seqLimit.isSelected()));
-    Cache.applicationProperties.setProperty("RIGHT_ALIGN_IDS",
+    Cache.setPropertyNoSave("RIGHT_ALIGN_IDS",
             Boolean.toString(rightAlign.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_FULLSCREEN",
+    Cache.setPropertyNoSave("SHOW_FULLSCREEN",
             Boolean.toString(fullScreen.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_OVERVIEW",
+    Cache.setPropertyNoSave("SHOW_OVERVIEW",
             Boolean.toString(openoverv.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_ANNOTATIONS",
+    Cache.setPropertyNoSave("SHOW_ANNOTATIONS",
             Boolean.toString(annotations.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_CONSERVATION",
+    Cache.setPropertyNoSave("SHOW_CONSERVATION",
             Boolean.toString(conservation.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_QUALITY",
+    Cache.setPropertyNoSave("SHOW_QUALITY",
             Boolean.toString(quality.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_IDENTITY",
+    Cache.setPropertyNoSave("SHOW_IDENTITY",
             Boolean.toString(identity.isSelected()));
 
-    Cache.applicationProperties.setProperty("GAP_SYMBOL",
+    Cache.setPropertyNoSave("GAP_SYMBOL",
             gapSymbolCB.getSelectedItem().toString());
 
-    Cache.applicationProperties.setProperty("FONT_NAME",
+    Cache.setPropertyNoSave("FONT_NAME",
             fontNameCB.getSelectedItem().toString());
-    Cache.applicationProperties.setProperty("FONT_STYLE",
+    Cache.setPropertyNoSave("FONT_STYLE",
             fontStyleCB.getSelectedItem().toString());
-    Cache.applicationProperties.setProperty("FONT_SIZE",
+    Cache.setPropertyNoSave("FONT_SIZE",
             fontSizeCB.getSelectedItem().toString());
 
-    Cache.applicationProperties.setProperty("ID_ITALICS",
+    Cache.setPropertyNoSave("ID_ITALICS",
             Boolean.toString(idItalics.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_UNCONSERVED",
+    Cache.setPropertyNoSave("SHOW_UNCONSERVED",
             Boolean.toString(showUnconserved.isSelected()));
-    Cache.applicationProperties.setProperty(SHOW_OCCUPANCY,
+    Cache.setPropertyNoSave(SHOW_OCCUPANCY,
             Boolean.toString(showOccupancy.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_GROUP_CONSENSUS",
+    Cache.setPropertyNoSave("SHOW_GROUP_CONSENSUS",
             Boolean.toString(showGroupConsensus.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_GROUP_CONSERVATION",
+    Cache.setPropertyNoSave("SHOW_GROUP_CONSERVATION",
             Boolean.toString(showGroupConservation.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_CONSENSUS_HISTOGRAM",
+    Cache.setPropertyNoSave("SHOW_CONSENSUS_HISTOGRAM",
             Boolean.toString(showConsensHistogram.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_CONSENSUS_LOGO",
+    Cache.setPropertyNoSave("SHOW_CONSENSUS_LOGO",
             Boolean.toString(showConsensLogo.isSelected()));
-    Cache.applicationProperties.setProperty("ANTI_ALIAS",
+    Cache.setPropertyNoSave("SHOW_INFORMATION_HISTOGRAM",
+            Boolean.toString(showConsensHistogram.isSelected()));
+    Cache.setPropertyNoSave("SHOW_HMM_LOGO",
+            Boolean.toString(showHMMLogo.isSelected()));
+    Cache.setPropertyNoSave("ANTI_ALIAS",
             Boolean.toString(smoothFont.isSelected()));
-    Cache.applicationProperties.setProperty(SCALE_PROTEIN_TO_CDNA,
+    Cache.setPropertyNoSave(SCALE_PROTEIN_TO_CDNA,
             Boolean.toString(scaleProteinToCdna.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_NPFEATS_TOOLTIP",
+    Cache.setPropertyNoSave("SHOW_NPFEATS_TOOLTIP",
             Boolean.toString(showNpTooltip.isSelected()));
-    Cache.applicationProperties.setProperty("SHOW_DBREFS_TOOLTIP",
+    Cache.setPropertyNoSave("SHOW_DBREFS_TOOLTIP",
             Boolean.toString(showDbRefTooltip.isSelected()));
 
-    Cache.applicationProperties.setProperty("WRAP_ALIGNMENT",
+    Cache.setPropertyNoSave("WRAP_ALIGNMENT",
             Boolean.toString(wrap.isSelected()));
 
-    Cache.applicationProperties.setProperty("STARTUP_FILE",
+    Cache.setPropertyNoSave("STARTUP_FILE",
             startupFileTextfield.getText());
-    Cache.applicationProperties.setProperty("SHOW_STARTUP_FILE",
+    Cache.setPropertyNoSave("SHOW_STARTUP_FILE",
             Boolean.toString(startupCheckbox.isSelected()));
 
-    Cache.applicationProperties.setProperty("SORT_ALIGNMENT",
+    Cache.setPropertyNoSave("SORT_ALIGNMENT",
             sortby.getSelectedItem().toString());
 
     // convert description of sort order to enum name for save
@@ -733,51 +1013,85 @@ public class Preferences extends GPreferences
             .forDescription(sortAnnBy.getSelectedItem().toString());
     if (annSortOrder != null)
     {
-      Cache.applicationProperties.setProperty(SORT_ANNOTATIONS,
+      Cache.setPropertyNoSave(SORT_ANNOTATIONS,
               annSortOrder.name());
     }
 
     final boolean showAutocalcFirst = sortAutocalc.getSelectedIndex() == 0;
-    Cache.applicationProperties.setProperty(SHOW_AUTOCALC_ABOVE,
+    Cache.setPropertyNoSave(SHOW_AUTOCALC_ABOVE,
             Boolean.valueOf(showAutocalcFirst).toString());
 
     /*
      * Save Colours settings
      */
-    Cache.applicationProperties.setProperty(DEFAULT_COLOUR_PROT,
+    Cache.setPropertyNoSave(DEFAULT_COLOUR_PROT,
             protColour.getSelectedItem().toString());
-    Cache.applicationProperties.setProperty(DEFAULT_COLOUR_NUC,
+    Cache.setPropertyNoSave(DEFAULT_COLOUR_NUC,
             nucColour.getSelectedItem().toString());
-    Cache.setColourProperty("ANNOTATIONCOLOUR_MIN",
+    Cache.setColourPropertyNoSave("ANNOTATIONCOLOUR_MIN",
             minColour.getBackground());
-    Cache.setColourProperty("ANNOTATIONCOLOUR_MAX",
+    Cache.setColourPropertyNoSave("ANNOTATIONCOLOUR_MAX",
             maxColour.getBackground());
 
     /*
+     * Save HMMER settings
+     */
+    Cache.setPropertyNoSave(HMMALIGN_TRIM_TERMINI,
+            Boolean.toString(hmmrTrimTermini.isSelected()));
+    Cache.setPropertyNoSave(HMMINFO_GLOBAL_BACKGROUND,
+            Boolean.toString(hmmerBackgroundUniprot.isSelected()));
+    Cache.setPropertyNoSave(HMMSEARCH_SEQCOUNT,
+            hmmerSequenceCount.getText());
+    Cache.setOrRemove(HMMER_PATH, hmmerPath.getText());
+    if (cygwinPath != null)
+    {
+      Cache.setOrRemove(CYGWIN_PATH, cygwinPath.getText());
+    }
+    AlignFrame[] frames = Desktop.getAlignFrames();
+    if (frames != null && frames.length > 0)
+    {
+      for (AlignFrame f : frames)
+      {
+        f.updateHMMERStatus();
+      }
+    }
+    
+    hmmrTrimTermini.setSelected(Cache.getDefault(HMMALIGN_TRIM_TERMINI, false));
+    if (Cache.getDefault(HMMINFO_GLOBAL_BACKGROUND, false))
+    {
+      hmmerBackgroundUniprot.setSelected(true);
+    }
+    else
+    {
+      hmmerBackgroundAlignment.setSelected(true);
+    }
+    hmmerSequenceCount
+            .setText(Cache.getProperty(HMMSEARCH_SEQCOUNT));
+    hmmerPath.setText(Cache.getProperty(HMMER_PATH));
+
+    /*
      * Save Overview settings
      */
-    Cache.setColourProperty(GAP_COLOUR, gapColour.getBackground());
-    Cache.setColourProperty(HIDDEN_COLOUR, hiddenColour.getBackground());
-    Cache.applicationProperties.setProperty(USE_LEGACY_GAP,
+    Cache.setColourPropertyNoSave(GAP_COLOUR, gapColour.getBackground());
+    Cache.setColourPropertyNoSave(HIDDEN_COLOUR, hiddenColour.getBackground());
+    Cache.setPropertyNoSave(USE_LEGACY_GAP,
             Boolean.toString(useLegacyGap.isSelected()));
-    Cache.applicationProperties.setProperty(SHOW_OV_HIDDEN_AT_START,
+    Cache.setPropertyNoSave(SHOW_OV_HIDDEN_AT_START,
             Boolean.toString(showHiddenAtStart.isSelected()));
 
     /*
      * Save Structure settings
      */
-    Cache.applicationProperties.setProperty(ADD_TEMPFACT_ANN,
+    Cache.setPropertyNoSave(ADD_TEMPFACT_ANN,
             Boolean.toString(addTempFactor.isSelected()));
-    Cache.applicationProperties.setProperty(ADD_SS_ANN,
+    Cache.setPropertyNoSave(ADD_SS_ANN,
             Boolean.toString(addSecondaryStructure.isSelected()));
-    Cache.applicationProperties.setProperty(USE_RNAVIEW,
-            Boolean.toString(useRnaView.isSelected()));
-    Cache.applicationProperties.setProperty(STRUCT_FROM_PDB,
+    Cache.setPropertyNoSave(STRUCT_FROM_PDB,
             Boolean.toString(structFromPdb.isSelected()));
+    if (!Platform.isJS()) {
     String viewer = structViewer.getSelectedItem().toString();
     String viewerPath = structureViewerPath.getText();
-    Cache.applicationProperties.setProperty(STRUCTURE_DISPLAY,
-            viewer);
+    Cache.setPropertyNoSave(STRUCTURE_DISPLAY, viewer);
     if (viewer.equals(ViewerType.CHIMERA.name()))
     {
       Cache.setOrRemove(CHIMERA_PATH, viewerPath);
@@ -790,23 +1104,25 @@ public class Preferences extends GPreferences
     {
       Cache.setOrRemove(PYMOL_PATH, viewerPath);
     }
-    Cache.applicationProperties.setProperty("MAP_WITH_SIFTS",
+    } // nojs
+    Cache.setPropertyNoSave("MAP_WITH_SIFTS",
             Boolean.toString(siftsMapping.isSelected()));
     SiftsSettings.setMapWithSifts(siftsMapping.isSelected());
 
     /*
      * Save Output settings
      */
-    Cache.applicationProperties.setProperty("EPS_RENDERING",
+    Cache.setPropertyNoSave("EPS_RENDERING",
             ((OptionsParam) epsRendering.getSelectedItem()).getCode());
-    Cache.applicationProperties.setProperty("HTML_RENDERING",
+    Cache.setPropertyNoSave("HTML_RENDERING",
             ((OptionsParam) htmlRendering.getSelectedItem()).getCode());
-    Cache.applicationProperties.setProperty("SVG_RENDERING",
+    Cache.setPropertyNoSave("SVG_RENDERING",
             ((OptionsParam) svgRendering.getSelectedItem()).getCode());
 
     /*
      * Save Connections settings
      */
+    // Proxy settings were already set first (to catch web services)
     Cache.setOrRemove("DEFAULT_BROWSER", defaultBrowser.getText());
 
     jalview.util.BrowserLauncher.resetBrowser();
@@ -815,43 +1131,28 @@ public class Preferences extends GPreferences
     String menuLinks = sequenceUrlLinks.writeUrlsAsString(true);
     if (menuLinks.isEmpty())
     {
-      Cache.applicationProperties.remove("SEQUENCE_LINKS");
+      Cache.removePropertyNoSave("SEQUENCE_LINKS");
     }
     else
     {
-      Cache.applicationProperties.setProperty("SEQUENCE_LINKS",
+      Cache.setPropertyNoSave("SEQUENCE_LINKS",
               menuLinks.toString());
     }
 
     String nonMenuLinks = sequenceUrlLinks.writeUrlsAsString(false);
     if (nonMenuLinks.isEmpty())
     {
-      Cache.applicationProperties.remove("STORED_LINKS");
+      Cache.removePropertyNoSave("STORED_LINKS");
     }
     else
     {
-      Cache.applicationProperties.setProperty("STORED_LINKS",
+      Cache.setPropertyNoSave("STORED_LINKS",
               nonMenuLinks.toString());
     }
 
-    Cache.applicationProperties.setProperty("DEFAULT_URL",
+    Cache.setPropertyNoSave("DEFAULT_URL",
             sequenceUrlLinks.getPrimaryUrlId());
 
-    // Proxy settings
-    Cache.applicationProperties.setProperty("USE_PROXY",
-            customProxy.isSelected() ? Cache.PROXYTYPE_CUSTOM
-                    : noProxy.isSelected() ? Cache.PROXYTYPE_NONE
-                            : Cache.PROXYTYPE_SYSTEM);
-    Cache.setOrRemove("PROXY_SERVER", proxyServerHttpTB.getText());
-    Cache.setOrRemove("PROXY_PORT", proxyPortHttpTB.getText());
-    Cache.setOrRemove("PROXY_SERVER_HTTPS", proxyServerHttpsTB.getText());
-    Cache.setOrRemove("PROXY_PORT_HTTPS", proxyPortHttpsTB.getText());
-    Cache.setOrRemove("PROXY_AUTH",
-            Boolean.toString(proxyAuth.isSelected()));
-    Cache.setOrRemove("PROXY_AUTH_USERNAME", proxyAuthUsernameTB.getText());
-    Cache.setOrRemove("PROXY_AUTH_PASSWORD", proxyAuthPasswordTB.getText());
-    setProxyFromSettings();
-
     Cache.setProperty("VERSION_CHECK",
             Boolean.toString(versioncheck.isSelected()));
     if (Cache.getProperty("USAGESTATS") != null || usagestats.isSelected())
@@ -874,40 +1175,40 @@ public class Preferences extends GPreferences
     /*
      * Save Output settings
      */
-    Cache.applicationProperties.setProperty("BLC_JVSUFFIX",
+    Cache.setPropertyNoSave("BLC_JVSUFFIX",
             Boolean.toString(blcjv.isSelected()));
-    Cache.applicationProperties.setProperty("CLUSTAL_JVSUFFIX",
+    Cache.setPropertyNoSave("CLUSTAL_JVSUFFIX",
             Boolean.toString(clustaljv.isSelected()));
-    Cache.applicationProperties.setProperty("FASTA_JVSUFFIX",
+    Cache.setPropertyNoSave("FASTA_JVSUFFIX",
             Boolean.toString(fastajv.isSelected()));
-    Cache.applicationProperties.setProperty("MSF_JVSUFFIX",
+    Cache.setPropertyNoSave("MSF_JVSUFFIX",
             Boolean.toString(msfjv.isSelected()));
-    Cache.applicationProperties.setProperty("PFAM_JVSUFFIX",
+    Cache.setPropertyNoSave("PFAM_JVSUFFIX",
             Boolean.toString(pfamjv.isSelected()));
-    Cache.applicationProperties.setProperty("PILEUP_JVSUFFIX",
+    Cache.setPropertyNoSave("PILEUP_JVSUFFIX",
             Boolean.toString(pileupjv.isSelected()));
-    Cache.applicationProperties.setProperty("PIR_JVSUFFIX",
+    Cache.setPropertyNoSave("PIR_JVSUFFIX",
             Boolean.toString(pirjv.isSelected()));
-    Cache.applicationProperties.setProperty("PIR_MODELLER",
+    Cache.setPropertyNoSave("PIR_MODELLER",
             Boolean.toString(modellerOutput.isSelected()));
-    Cache.applicationProperties.setProperty("EXPORT_EMBBED_BIOJSON",
+    Cache.setPropertyNoSave("EXPORT_EMBBED_BIOJSON",
             Boolean.toString(embbedBioJSON.isSelected()));
     jalview.io.PIRFile.useModellerOutput = modellerOutput.isSelected();
 
-    Cache.applicationProperties.setProperty("FIGURE_AUTOIDWIDTH",
+    Cache.setPropertyNoSave("FIGURE_AUTOIDWIDTH",
             Boolean.toString(autoIdWidth.isSelected()));
     userIdWidth_actionPerformed();
-    Cache.applicationProperties.setProperty("FIGURE_FIXEDIDWIDTH",
+    Cache.setPropertyNoSave("FIGURE_FIXEDIDWIDTH",
             userIdWidth.getText());
 
     /*
      * Save Editing settings
      */
-    Cache.applicationProperties.setProperty("AUTO_CALC_CONSENSUS",
+    Cache.setPropertyNoSave("AUTO_CALC_CONSENSUS",
             Boolean.toString(autoCalculateConsCheck.isSelected()));
-    Cache.applicationProperties.setProperty("SORT_BY_TREE",
+    Cache.setPropertyNoSave("SORT_BY_TREE",
             Boolean.toString(sortByTree.isSelected()));
-    Cache.applicationProperties.setProperty("PAD_GAPS",
+    Cache.setPropertyNoSave("PAD_GAPS",
             Boolean.toString(padGaps.isSelected()));
 
     if (!Platform.isJS())
@@ -918,28 +1219,41 @@ public class Preferences extends GPreferences
     /*
      * Save Backups settings
      */
-    Cache.applicationProperties.setProperty(BackupFiles.ENABLED,
+    Cache.setPropertyNoSave(BackupFiles.ENABLED,
             Boolean.toString(enableBackupFiles.isSelected()));
     int preset = getComboIntStringKey(backupfilesPresetsCombo);
-    Cache.applicationProperties.setProperty(BackupFiles.NS + "_PRESET", Integer.toString(preset));
+    Cache.setPropertyNoSave(BackupFiles.NS + "_PRESET", Integer.toString(preset));
 
     if (preset == BackupFilesPresetEntry.BACKUPFILESSCHEMECUSTOM)
     {
       BackupFilesPresetEntry customBFPE = getBackupfilesCurrentEntry();
       BackupFilesPresetEntry.backupfilesPresetEntriesValues.put(
               BackupFilesPresetEntry.BACKUPFILESSCHEMECUSTOM, customBFPE);
-      Cache.applicationProperties
-              .setProperty(BackupFilesPresetEntry.CUSTOMCONFIG,
+      Cache.setPropertyNoSave(BackupFilesPresetEntry.CUSTOMCONFIG,
                       customBFPE.toString());
     }
 
     BackupFilesPresetEntry savedBFPE = BackupFilesPresetEntry.backupfilesPresetEntriesValues
             .get(preset);
-    Cache.applicationProperties.setProperty(
+    Cache.setPropertyNoSave(
             BackupFilesPresetEntry.SAVEDCONFIG, savedBFPE.toString());
 
+    /*
+     * Save Memory Settings
+     */
+    Cache.setPropertyNoSave(
+            MemorySetting.CUSTOMISED_SETTINGS,
+            Boolean.toString(customiseMemorySetting.isSelected()));
+    Cache.setPropertyNoSave(MemorySetting.MEMORY_JVMMEMPC,
+            Integer.toString(jvmMemoryPercentSlider.getValue()));
+    Cache.setPropertyNoSave(MemorySetting.MEMORY_JVMMEMMAX,
+            jvmMemoryMaxTextField.getText());
+
+    /*
+     * save and close Preferences
+     */
     Cache.saveProperties();
-    Desktop.instance.doConfigureStructurePrefs();
+    Desktop.getInstance().doConfigureStructurePrefs();
     try
     {
       frame.setClosed(true);
@@ -948,40 +1262,67 @@ public class Preferences extends GPreferences
     }
   }
 
-  public void setProxyFromSettings()
+  public void saveProxySettings()
   {
-    String proxyType = Cache.getDefault("USE_PROXY",
-            Cache.PROXYTYPE_SYSTEM);
-    if (proxyType.equals(Cache.PROXYTYPE_NONE))
-    {
-      if (!previousProxyType.equals(proxyType))
-        Cache.log.info("Setting no proxy settings");
-      Cache.setProxyProperties(null, null, null, null, null, null, null,
-              null);
-    }
-    else if (proxyType.equals(Cache.PROXYTYPE_CUSTOM))
-    {
-      if (!previousProxyType.equals(proxyType))
-        Cache.log.info("Setting custom proxy settings");
-      boolean proxyAuthSet = Cache.getDefault("PROXY_AUTH", false);
-      Cache.setProxyProperties(Cache.getDefault("PROXY_SERVER", null),
-              Cache.getDefault("PROXY_PORT", null),
-              Cache.getDefault("PROXY_SERVER_HTTPS", null),
-              Cache.getDefault("PROXY_PORT_HTTPS", null),
-              proxyAuthSet ? Cache.getDefault("PROXY_AUTH_USERNAME", "")
-                      : null,
-              proxyAuthSet ? Cache.getDefault("PROXY_AUTH_PASSWORD", "")
-                      : null);
-    }
-    else // systemProxy should be selected and is sensible default anyway
-    {
-      if (!previousProxyType.equals(proxyType))
-        Cache.log.info("Setting system proxy settings");
-      Cache.resetProxyProperties();
+    String newProxyType = customProxy.isSelected() ? Cache.PROXYTYPE_CUSTOM
+            : noProxy.isSelected() ? Cache.PROXYTYPE_NONE
+                    : Cache.PROXYTYPE_SYSTEM;
+    Cache.setPropertyNoSave("USE_PROXY", newProxyType);
+    Cache.setOrRemove("PROXY_SERVER", proxyServerHttpTB.getText());
+    Cache.setOrRemove("PROXY_PORT", proxyPortHttpTB.getText());
+    Cache.setOrRemove("PROXY_SERVER_HTTPS", proxyServerHttpsTB.getText());
+    Cache.setOrRemove("PROXY_PORT_HTTPS", proxyPortHttpsTB.getText());
+    Cache.setOrRemove("PROXY_AUTH",
+            Boolean.toString(proxyAuth.isSelected()));
+    Cache.setOrRemove("PROXY_AUTH_USERNAME", proxyAuthUsernameTB.getText());
+    Cache.proxyAuthPassword = proxyAuthPasswordPB.getPassword();
+    Cache.setProxyPropertiesFromPreferences(previousProxyType);
+    if (newProxyType.equals(Cache.PROXYTYPE_CUSTOM)
+            || !newProxyType.equals(previousProxyType))
+    {
+      // force a re-lookup of ws if proxytype is custom or has changed
+      wsPrefs.refreshWs_actionPerformed(null);
     }
+    previousProxyType = newProxyType;
   }
 
-  /**
+  public static void setAppletDefaults()
+  {
+
+    // http://www.jalview.org/old/v2_8/examples/appletParameters.html
+
+    // showConservation true or false Default is true.
+    // showQuality true or false Default is true.
+    // showConsensus true or false Default is true.
+    // showFeatureSettings true or false Shows the feature settings window when
+    // startin
+    // showTreeBootstraps true or false (default is true) show or hide branch
+    // bootstraps
+    // showTreeDistances true or false (default is true) show or hide branch
+    // lengths
+    // showUnlinkedTreeNodes true or false (default is false) indicate if
+    // unassociated nodes should be highlighted in the tree view
+    // showUnconserved true of false (default is false) When true, only gaps and
+    // symbols different to the consensus sequence ions of the alignment
+    // showGroupConsensus true of false (default is false) When true, shows
+    // consensus annotation row for any groups on the alignment. (since 2.7)
+    // showGroupConservation true of false (default is false) When true, shows
+    // amino-acid property conservation annotation row for any groups on the
+    // showConsensusHistogram true of false (default is true) When true, shows
+    // the percentage occurence of the consensus symbol for each column as a
+    // showSequenceLogo true of false (default is false) When true, shows a
+    // sequence logo above the consensus sequence (overlaid above the Consensus
+
+    Cache.setPropertyNoSave(SHOW_CONSERVATION, "true");
+    Cache.setPropertyNoSave(SHOW_QUALITY, "false");
+    Cache.setPropertyNoSave(SHOW_CONSENSUS, "true");
+    Cache.setPropertyNoSave(SHOW_UNCONSERVED, "false");
+    Cache.setPropertyNoSave(SHOW_GROUP_CONSERVATION, "false");
+    Cache.setPropertyNoSave(SHOW_GROUP_CONSENSUS, "false");
+
+    // TODO -- just a start here
+  }
+ /**
    * Do any necessary validation before saving settings. Return focus to the
    * first tab which fails validation.
    * 
@@ -1025,7 +1366,7 @@ public class Preferences extends GPreferences
       FileFormatI format = chooser.getSelectedFormat();
       if (format != null)
       {
-        Cache.applicationProperties.setProperty("DEFAULT_FILE_FORMAT",
+        Cache.setPropertyNoSave("DEFAULT_FILE_FORMAT",
                 format.getName());
       }
       startupFileTextfield
@@ -1074,6 +1415,8 @@ public class Preferences extends GPreferences
             && (identity.isSelected() || showGroupConsensus.isSelected()));
     showConsensLogo.setEnabled(annotations.isSelected()
             && (identity.isSelected() || showGroupConsensus.isSelected()));
+    showInformationHistogram.setEnabled(annotations.isSelected());
+    showHMMLogo.setEnabled(annotations.isSelected());
   }
 
   @Override
@@ -1083,7 +1426,7 @@ public class Preferences extends GPreferences
     boolean valid = false;
     while (!valid)
     {
-      if (JvOptionPane.showInternalConfirmDialog(Desktop.desktop, link,
+      if (JvOptionPane.showInternalConfirmDialog(Desktop.getDesktopPane(), link,
               MessageManager.getString("label.new_sequence_url_link"),
               JvOptionPane.OK_CANCEL_OPTION, -1,
               null) == JvOptionPane.OK_OPTION)
@@ -1120,7 +1463,7 @@ public class Preferences extends GPreferences
     if (index == -1)
     {
       // button no longer enabled if row is not selected
-      Cache.log.debug("Edit with no row selected in linkUrlTable");
+      Console.debug("Edit with no row selected in linkUrlTable");
       return;
     }
 
@@ -1135,7 +1478,7 @@ public class Preferences extends GPreferences
     boolean valid = false;
     while (!valid)
     {
-      if (JvOptionPane.showInternalConfirmDialog(Desktop.desktop, link,
+      if (JvOptionPane.showInternalConfirmDialog(Desktop.getDesktopPane(), link,
               MessageManager.getString("label.edit_sequence_url_link"),
               JvOptionPane.OK_CANCEL_OPTION, -1,
               null) == JvOptionPane.OK_OPTION)
@@ -1172,7 +1515,7 @@ public class Preferences extends GPreferences
     if (index == -1)
     {
       // button no longer enabled if row is not selected
-      Cache.log.debug("Delete with no row selected in linkUrlTable");
+      Console.debug("Delete with no row selected in linkUrlTable");
       return;
     }
     else
@@ -1308,7 +1651,7 @@ public class Preferences extends GPreferences
     } catch (NumberFormatException x)
     {
       userIdWidth.setText("");
-      JvOptionPane.showInternalMessageDialog(Desktop.desktop,
+      JvOptionPane.showInternalMessageDialog(Desktop.getDesktopPane(),
               MessageManager
                       .getString("warn.user_defined_width_requirements"),
               MessageManager.getString("label.invalid_id_column_width"),
@@ -1324,8 +1667,8 @@ public class Preferences extends GPreferences
   }
 
   /**
-   * Returns true if structure viewer path is to a valid executable, else shows
-   * an error dialog. Does nothing if the path is empty, as is the case for Jmol
+   * Returns true if structure viewer path is to a valid executable, else shows an
+   * error dialog. Does nothing if the path is empty, as is the case for Jmol
    * (built in to Jalview) or when Jalview is left to try default paths.
    */
   private boolean validateViewerPath()
@@ -1335,7 +1678,7 @@ public class Preferences extends GPreferences
       File f = new File(structureViewerPath.getText());
       if (!f.canExecute())
       {
-        JvOptionPane.showInternalMessageDialog(Desktop.desktop,
+        JvOptionPane.showInternalMessageDialog(Desktop.getDesktopPane(),
                 MessageManager.getString("label.invalid_viewer_path"),
                 MessageManager.getString("label.invalid_viewer_path"),
                 JvOptionPane.ERROR_MESSAGE);
@@ -1344,10 +1687,61 @@ public class Preferences extends GPreferences
     }
     return true;
   }
+  
+  /**
+   * Returns true if the given text field contains a path to a folder that
+   * contains an executable with the given name, else false (after showing a
+   * warning dialog). The executable name will be tried with .exe appended if not
+   * found.
+   * 
+   * @param textField
+   * @param executable
+   */
+  protected boolean validateExecutablePath(JTextField textField, String executable)
+  {
+    String folder = textField.getText().trim();
+
+    if (FileUtils.getExecutable(executable, folder) != null)
+    {
+      return true;
+    }
+    if (folder.length() > 0)
+    {
+      JvOptionPane.showInternalMessageDialog(Desktop.getInstance(),
+              MessageManager.formatMessage("label.executable_not_found",
+                      executable),
+              MessageManager.getString("label.invalid_folder"),
+              JvOptionPane.ERROR_MESSAGE);
+    }
+    return false;
+  }
+
+  /**
+   * Checks if a file can be executed
+   * 
+   * @param path
+   *          the path to the file
+   * @return
+   */
+  public boolean canExecute(String path)
+  {
+    File file = new File(path);
+    if (!file.canExecute())
+    {
+      file = new File(path + ".exe");
+      {
+        if (!file.canExecute())
+        {
+          return false;
+        }
+      }
+    }
+    return true;
+  }
 
   /**
-   * If Chimera or ChimeraX or Pymol is selected, check it can be found on
-   * default or user-specified path, if not show a warning/help dialog
+   * If Chimera or ChimeraX or Pymol is selected, check it can be found on default
+   * or user-specified path, if not show a warning/help dialog
    */
   @Override
   protected void structureViewer_actionPerformed(String selectedItem)
@@ -1409,13 +1803,15 @@ public class Preferences extends GPreferences
     if (!found)
     {
       String[] options = { "OK", "Help" };
-      int showHelp = JvOptionPane.showInternalOptionDialog(Desktop.desktop,
+      int showHelp = JvOptionPane.showInternalOptionDialog(Desktop.getDesktopPane(),
               JvSwingUtils.wrapTooltip(true,
                       MessageManager.getString("label.viewer_missing")),
               "", JvOptionPane.YES_NO_OPTION, JvOptionPane.WARNING_MESSAGE,
               null, options, options[0]);
+
       if (showHelp == JvOptionPane.NO_OPTION)
       {
+        this.selectTab(Preferences.TabRef.STRUCTURE_TAB, null);
         try
         {
           Help.showHelpWindow(HelpId.StructureViewer);
@@ -1424,7 +1820,37 @@ public class Preferences extends GPreferences
           e.printStackTrace();
         }
       }
-    }
+      else if (showHelp == JvOptionPane.OK_OPTION)
+      {
+        this.selectTab(Preferences.TabRef.STRUCTURE_TAB, null);
+        CompletableFuture<Void> cf = CompletableFuture.runAsync(() -> {
+          try
+          {
+            for (int i = 0; i < 3; i++)
+            {
+              structureViewerPath.setBackground(Color.PINK);
+              Thread.sleep(500);
+              structureViewerPath.setBackground(Color.WHITE);
+              Thread.sleep(500);
+            }
+          } catch (InterruptedException e)
+          {
+          }
+        });
+      }
+     }
+  }
+
+  @Override
+  protected void validateHmmerPath()
+  {
+    validateExecutablePath(hmmerPath, HmmerCommand.HMMBUILD);
+  }
+
+  @Override
+  protected void validateCygwinPath()
+  {
+    validateExecutablePath(cygwinPath, "run");
   }
 
   public class OptionsParam