Merge branch 'JAL-1956_featureStyles' into features/JAL-653_JAL-1766_htslib_refseqsupport
[jalview.git] / test / jalview / analysis / AlignmentUtilsTests.java
index 2beacfe..a82a881 100644 (file)
@@ -36,6 +36,7 @@ import jalview.datamodel.Mapping;
 import jalview.datamodel.SearchResults;
 import jalview.datamodel.SearchResults.Match;
 import jalview.datamodel.Sequence;
+import jalview.datamodel.SequenceFeature;
 import jalview.datamodel.SequenceI;
 import jalview.io.AppletFormatAdapter;
 import jalview.io.FormatAdapter;
@@ -45,10 +46,8 @@ import jalview.util.MappingUtils;
 import java.io.IOException;
 import java.util.ArrayList;
 import java.util.Arrays;
-import java.util.Collections;
 import java.util.HashSet;
 import java.util.Iterator;
-import java.util.LinkedHashSet;
 import java.util.List;
 import java.util.Map;
 import java.util.Set;
@@ -281,7 +280,7 @@ public class AlignmentUtilsTests
    * @throws IOException
    */
   @Test(groups = { "Functional" })
-  public void testMapProteinToCdna_noXrefs() throws IOException
+  public void testMapProteinAlignmentToCdna_noXrefs() throws IOException
   {
     List<SequenceI> protseqs = new ArrayList<SequenceI>();
     protseqs.add(new Sequence("UNIPROT|V12345", "EIQ"));
@@ -298,7 +297,7 @@ public class AlignmentUtilsTests
     AlignmentI cdna = new Alignment(dnaseqs.toArray(new SequenceI[4]));
     cdna.setDataset(null);
 
-    assertTrue(AlignmentUtils.mapProteinToCdna(protein, cdna));
+    assertTrue(AlignmentUtils.mapProteinAlignmentToCdna(protein, cdna));
 
     // 3 mappings made, each from 1 to 1 sequence
     assertEquals(3, protein.getCodonFrames().size());
@@ -372,8 +371,8 @@ public class AlignmentUtilsTests
      * region). The leading gap, and the gaps between codons, are subsumed by
      * the protein alignment gap.
      */
-    checkAlignSequenceAs("-G-GG--AA-A-", "-A-L-", true, true, map,
-            "---G-GG---AA-A-");
+    checkAlignSequenceAs("-G-GG--AA-A---", "-A-L-", true, true, map,
+            "---G-GG---AA-A---");
 
     /*
      * Include only unmapped gaps in dna when realigning (outside the exon
@@ -381,7 +380,7 @@ public class AlignmentUtilsTests
      * the protein alignment gap.
      */
     checkAlignSequenceAs("-G-GG--AA-A-", "-A-L-", false, true, map,
-            "---GGG---AAA-");
+            "---GGG---AAA---");
   }
 
   /**
@@ -439,7 +438,6 @@ public class AlignmentUtilsTests
   @Test(groups = { "Functional" })
   public void testAlignSequenceAs_withMapping_withUnmappedProtein()
   {
-
     /*
      * Exons at codon 2 (AAA) and 4 (TTT) mapped to A and P
      */
@@ -447,38 +445,39 @@ public class AlignmentUtilsTests
         1, 1, 3, 3 }, 3, 1);
 
     /*
-     * Expect alignment does nothing (aborts realignment). Change this test
-     * first if different behaviour wanted.
+     * -L- 'aligns' ccc------
      */
-    checkAlignSequenceAs("GGGAAACCCTTTGGG", "-A-L-P-", false, false, map,
-            "GGGAAACCCTTTGGG");
+    checkAlignSequenceAs("gggAAAcccTTTggg", "-A-L-P-", false, false, map,
+            "gggAAAccc------TTTggg");
   }
 
   /**
    * Helper method that performs and verifies the method under test.
    * 
-   * @param dnaSeq
-   * @param proteinSeq
+   * @param alignee
+   *          the sequence to be realigned
+   * @param alignModel
+   *          the sequence whose alignment is to be copied
    * @param preserveMappedGaps
    * @param preserveUnmappedGaps
    * @param map
    * @param expected
    */
-  protected void checkAlignSequenceAs(final String dnaSeq,
-          final String proteinSeq, final boolean preserveMappedGaps,
+  protected void checkAlignSequenceAs(final String alignee,
+          final String alignModel, final boolean preserveMappedGaps,
           final boolean preserveUnmappedGaps, MapList map,
           final String expected)
   {
-    SequenceI dna = new Sequence("Seq1", dnaSeq);
-    dna.createDatasetSequence();
-    SequenceI protein = new Sequence("Seq1", proteinSeq);
-    protein.createDatasetSequence();
+    SequenceI alignMe = new Sequence("Seq1", alignee);
+    alignMe.createDatasetSequence();
+    SequenceI alignFrom = new Sequence("Seq2", alignModel);
+    alignFrom.createDatasetSequence();
     AlignedCodonFrame acf = new AlignedCodonFrame();
-    acf.addMap(dna.getDatasetSequence(), protein.getDatasetSequence(), map);
+    acf.addMap(alignMe.getDatasetSequence(), alignFrom.getDatasetSequence(), map);
 
-    AlignmentUtils.alignSequenceAs(dna, protein, acf, "---", '-',
+    AlignmentUtils.alignSequenceAs(alignMe, alignFrom, acf, "---", '-',
             preserveMappedGaps, preserveUnmappedGaps);
-    assertEquals(expected, dna.getSequenceAsString());
+    assertEquals(expected, alignMe.getSequenceAsString());
   }
 
   /**
@@ -550,7 +549,9 @@ public class AlignmentUtilsTests
     acf.addMap(dna1.getDatasetSequence(), prot1.getDatasetSequence(), map);
     acf.addMap(dna2.getDatasetSequence(), prot2.getDatasetSequence(), map);
     acf.addMap(dna3.getDatasetSequence(), prot3.getDatasetSequence(), map);
-    protein.setCodonFrames(Collections.singleton(acf));
+    ArrayList<AlignedCodonFrame> acfs = new ArrayList<AlignedCodonFrame>();
+    acfs.add(acf);
+    protein.setCodonFrames(acfs);
 
     /*
      * Translated codon order is [1,2,3] [1,3,4] [4,5,6] [4,5,7] [5,6,7] [7,8,9]
@@ -617,7 +618,7 @@ public class AlignmentUtilsTests
    * @throws IOException
    */
   @Test(groups = { "Functional" })
-  public void testMapProteinToCdna_withStartAndStopCodons()
+  public void testMapProteinAlignmentToCdna_withStartAndStopCodons()
           throws IOException
   {
     List<SequenceI> protseqs = new ArrayList<SequenceI>();
@@ -638,7 +639,7 @@ public class AlignmentUtilsTests
     AlignmentI cdna = new Alignment(dnaseqs.toArray(new SequenceI[4]));
     cdna.setDataset(null);
 
-    assertTrue(AlignmentUtils.mapProteinToCdna(protein, cdna));
+    assertTrue(AlignmentUtils.mapProteinAlignmentToCdna(protein, cdna));
 
     // 3 mappings made, each from 1 to 1 sequence
     assertEquals(3, protein.getCodonFrames().size());
@@ -710,7 +711,7 @@ public class AlignmentUtilsTests
    * @throws IOException
    */
   @Test(groups = { "Functional" })
-  public void testMapProteinToCdna_withXrefs() throws IOException
+  public void testMapProteinAlignmentToCdna_withXrefs() throws IOException
   {
     List<SequenceI> protseqs = new ArrayList<SequenceI>();
     protseqs.add(new Sequence("UNIPROT|V12345", "EIQ"));
@@ -739,7 +740,7 @@ public class AlignmentUtilsTests
     // A11111 should be mapped to V12347
     // A55555 is spare and has no xref so is not mapped
 
-    assertTrue(AlignmentUtils.mapProteinToCdna(protein, cdna));
+    assertTrue(AlignmentUtils.mapProteinAlignmentToCdna(protein, cdna));
 
     // 4 protein mappings made for 3 proteins, 2 to V12345, 1 each to V12346/7
     assertEquals(3, protein.getCodonFrames().size());
@@ -786,7 +787,8 @@ public class AlignmentUtilsTests
    * @throws IOException
    */
   @Test(groups = { "Functional" })
-  public void testMapProteinToCdna_prioritiseXrefs() throws IOException
+  public void testMapProteinAlignmentToCdna_prioritiseXrefs()
+          throws IOException
   {
     List<SequenceI> protseqs = new ArrayList<SequenceI>();
     protseqs.add(new Sequence("UNIPROT|V12345", "EIQ"));
@@ -806,7 +808,7 @@ public class AlignmentUtilsTests
     // A11111 should then be mapped to the unmapped V12346
     dnaseqs.get(1).addDBRef(new DBRefEntry("UNIPROT", "1", "V12345"));
 
-    assertTrue(AlignmentUtils.mapProteinToCdna(protein, cdna));
+    assertTrue(AlignmentUtils.mapProteinAlignmentToCdna(protein, cdna));
 
     // 2 protein mappings made
     assertEquals(2, protein.getCodonFrames().size());
@@ -987,7 +989,7 @@ public class AlignmentUtilsTests
     assertTrue(AlignmentUtils.haveCrossRef(seq2, seq1));
 
     // now the other way round
-    seq1.setDBRef(null);
+    seq1.setDBRefs(null);
     seq2.addDBRef(new DBRefEntry("EMBL", "1", "A12345"));
     assertTrue(AlignmentUtils.haveCrossRef(seq1, seq2));
     assertTrue(AlignmentUtils.haveCrossRef(seq2, seq1));
@@ -999,10 +1001,10 @@ public class AlignmentUtilsTests
   }
 
   /**
-   * Test the method that extracts the exon-only part of a dna alignment.
+   * Test the method that extracts the cds-only part of a dna alignment.
    */
   @Test(groups = { "Functional" })
-  public void testMakeExonAlignment()
+  public void testMakeCdsAlignment()
   {
     SequenceI dna1 = new Sequence("dna1", "aaaGGGcccTTTaaa");
     SequenceI dna2 = new Sequence("dna2", "GGGcccTTTaaaCCC");
@@ -1013,7 +1015,7 @@ public class AlignmentUtilsTests
     pep1.createDatasetSequence();
     pep2.createDatasetSequence();
 
-    Set<AlignedCodonFrame> mappings = new HashSet<AlignedCodonFrame>();
+    List<AlignedCodonFrame> mappings = new ArrayList<AlignedCodonFrame>();
     MapList map = new MapList(new int[] { 4, 6, 10, 12 },
             new int[] { 1, 2 }, 3, 1);
     AlignedCodonFrame acf = new AlignedCodonFrame();
@@ -1025,11 +1027,11 @@ public class AlignmentUtilsTests
     acf.addMap(dna2.getDatasetSequence(), pep2.getDatasetSequence(), map);
     mappings.add(acf);
 
-    AlignmentI exons = AlignmentUtils.makeExonAlignment(new SequenceI[] {
+    AlignmentI cds = AlignmentUtils.makeCdsAlignment(new SequenceI[] {
         dna1, dna2 }, mappings);
-    assertEquals(2, exons.getSequences().size());
-    assertEquals("GGGTTT", exons.getSequenceAt(0).getSequenceAsString());
-    assertEquals("GGGTTTCCC", exons.getSequenceAt(1).getSequenceAsString());
+    assertEquals(2, cds.getSequences().size());
+    assertEquals("GGGTTT", cds.getSequenceAt(0).getSequenceAsString());
+    assertEquals("GGGTTTCCC", cds.getSequenceAt(1).getSequenceAsString());
 
     /*
      * Verify updated mappings
@@ -1046,14 +1048,14 @@ public class AlignmentUtilsTests
     SearchResults sr = MappingUtils.buildSearchResults(pep1, 1, mappings);
     assertEquals(1, sr.getResults().size());
     Match m = sr.getResults().get(0);
-    assertEquals(exons.getSequenceAt(0).getDatasetSequence(),
+    assertEquals(cds.getSequenceAt(0).getDatasetSequence(),
             m.getSequence());
     assertEquals(1, m.getStart());
     assertEquals(3, m.getEnd());
     // map F to TTT
     sr = MappingUtils.buildSearchResults(pep1, 2, mappings);
     m = sr.getResults().get(0);
-    assertEquals(exons.getSequenceAt(0).getDatasetSequence(),
+    assertEquals(cds.getSequenceAt(0).getDatasetSequence(),
             m.getSequence());
     assertEquals(4, m.getStart());
     assertEquals(6, m.getEnd());
@@ -1068,34 +1070,34 @@ public class AlignmentUtilsTests
     sr = MappingUtils.buildSearchResults(pep2, 1, mappings);
     assertEquals(1, sr.getResults().size());
     m = sr.getResults().get(0);
-    assertEquals(exons.getSequenceAt(1).getDatasetSequence(),
+    assertEquals(cds.getSequenceAt(1).getDatasetSequence(),
             m.getSequence());
     assertEquals(1, m.getStart());
     assertEquals(3, m.getEnd());
     // map F to TTT
     sr = MappingUtils.buildSearchResults(pep2, 2, mappings);
     m = sr.getResults().get(0);
-    assertEquals(exons.getSequenceAt(1).getDatasetSequence(),
+    assertEquals(cds.getSequenceAt(1).getDatasetSequence(),
             m.getSequence());
     assertEquals(4, m.getStart());
     assertEquals(6, m.getEnd());
     // map P to CCC
     sr = MappingUtils.buildSearchResults(pep2, 3, mappings);
     m = sr.getResults().get(0);
-    assertEquals(exons.getSequenceAt(1).getDatasetSequence(),
+    assertEquals(cds.getSequenceAt(1).getDatasetSequence(),
             m.getSequence());
     assertEquals(7, m.getStart());
     assertEquals(9, m.getEnd());
   }
 
   /**
-   * Test the method that makes an exon-only sequence from a DNA sequence and
-   * its product mapping. Test includes the expected case that the DNA sequence
+   * Test the method that makes a cds-only sequence from a DNA sequence and its
+   * product mapping. Test includes the expected case that the DNA sequence
    * already has a protein product (Uniprot translation) which in turn has an
    * x-ref to the EMBLCDS record.
    */
   @Test(groups = { "Functional" })
-  public void testMakeExonSequences()
+  public void testMakeCdsSequences()
   {
     SequenceI dna1 = new Sequence("dna1", "aaaGGGcccTTTaaa");
     SequenceI pep1 = new Sequence("pep1", "GF");
@@ -1116,27 +1118,27 @@ public class AlignmentUtilsTests
     mappings.add(acf);
 
     AlignedCodonFrame newMapping = new AlignedCodonFrame();
-    List<SequenceI> exons = AlignmentUtils.makeExonSequences(dna1, acf,
+    List<SequenceI> cdsSeqs = AlignmentUtils.makeCdsSequences(dna1, acf,
             newMapping);
-    assertEquals(1, exons.size());
-    SequenceI exon = exons.get(0);
+    assertEquals(1, cdsSeqs.size());
+    SequenceI cdsSeq = cdsSeqs.get(0);
 
-    assertEquals("GGGTTT", exon.getSequenceAsString());
-    assertEquals("dna1|A12345", exon.getName());
-    assertEquals(1, exon.getDBRef().length);
-    DBRefEntry cdsRef = exon.getDBRef()[0];
+    assertEquals("GGGTTT", cdsSeq.getSequenceAsString());
+    assertEquals("dna1|A12345", cdsSeq.getName());
+    assertEquals(1, cdsSeq.getDBRefs().length);
+    DBRefEntry cdsRef = cdsSeq.getDBRefs()[0];
     assertEquals("EMBLCDS", cdsRef.getSource());
     assertEquals("2", cdsRef.getVersion());
     assertEquals("A12345", cdsRef.getAccessionId());
   }
 
   /**
-   * Test the method that makes an exon-only alignment from a DNA sequence and
-   * its product mappings, for the case where there are multiple exon mappings
-   * to different protein products.
+   * Test the method that makes a cds-only alignment from a DNA sequence and its
+   * product mappings, for the case where there are multiple exon mappings to
+   * different protein products.
    */
   @Test(groups = { "Functional" })
-  public void testMakeExonAlignment_multipleProteins()
+  public void testMakeCdsAlignment_multipleProteins()
   {
     SequenceI dna1 = new Sequence("dna1", "aaaGGGcccTTTaaa");
     SequenceI pep1 = new Sequence("pep1", "GF"); // GGGTTT
@@ -1158,7 +1160,7 @@ public class AlignmentUtilsTests
      * convenience so results are in the input order. There is no assertion that
      * the generated exon sequences are in any particular order.
      */
-    Set<AlignedCodonFrame> mappings = new LinkedHashSet<AlignedCodonFrame>();
+    List<AlignedCodonFrame> mappings = new ArrayList<AlignedCodonFrame>();
     // map ...GGG...TTT to GF
     MapList map = new MapList(new int[] { 4, 6, 10, 12 },
             new int[] { 1, 2 }, 3, 1);
@@ -1182,82 +1184,82 @@ public class AlignmentUtilsTests
      * Create the Exon alignment; also replaces the dna-to-protein mappings with
      * exon-to-protein and exon-to-dna mappings
      */
-    AlignmentI exal = AlignmentUtils.makeExonAlignment(
+    AlignmentI exal = AlignmentUtils.makeCdsAlignment(
             new SequenceI[] { dna1 }, mappings);
 
     /*
-     * Verify we have 3 exon sequences, mapped to pep1/2/3 respectively
+     * Verify we have 3 cds sequences, mapped to pep1/2/3 respectively
      */
-    List<SequenceI> exons = exal.getSequences();
-    assertEquals(3, exons.size());
-
-    SequenceI exon = exons.get(0);
-    assertEquals("GGGTTT", exon.getSequenceAsString());
-    assertEquals("dna1|A12345", exon.getName());
-    assertEquals(1, exon.getDBRef().length);
-    DBRefEntry cdsRef = exon.getDBRef()[0];
+    List<SequenceI> cds = exal.getSequences();
+    assertEquals(3, cds.size());
+
+    SequenceI cdsSeq = cds.get(0);
+    assertEquals("GGGTTT", cdsSeq.getSequenceAsString());
+    assertEquals("dna1|A12345", cdsSeq.getName());
+    assertEquals(1, cdsSeq.getDBRefs().length);
+    DBRefEntry cdsRef = cdsSeq.getDBRefs()[0];
     assertEquals("EMBLCDS", cdsRef.getSource());
     assertEquals("2", cdsRef.getVersion());
     assertEquals("A12345", cdsRef.getAccessionId());
 
-    exon = exons.get(1);
-    assertEquals("aaaccc", exon.getSequenceAsString());
-    assertEquals("dna1|A12346", exon.getName());
-    assertEquals(1, exon.getDBRef().length);
-    cdsRef = exon.getDBRef()[0];
+    cdsSeq = cds.get(1);
+    assertEquals("aaaccc", cdsSeq.getSequenceAsString());
+    assertEquals("dna1|A12346", cdsSeq.getName());
+    assertEquals(1, cdsSeq.getDBRefs().length);
+    cdsRef = cdsSeq.getDBRefs()[0];
     assertEquals("EMBLCDS", cdsRef.getSource());
     assertEquals("3", cdsRef.getVersion());
     assertEquals("A12346", cdsRef.getAccessionId());
 
-    exon = exons.get(2);
-    assertEquals("aaaTTT", exon.getSequenceAsString());
-    assertEquals("dna1|A12347", exon.getName());
-    assertEquals(1, exon.getDBRef().length);
-    cdsRef = exon.getDBRef()[0];
+    cdsSeq = cds.get(2);
+    assertEquals("aaaTTT", cdsSeq.getSequenceAsString());
+    assertEquals("dna1|A12347", cdsSeq.getName());
+    assertEquals(1, cdsSeq.getDBRefs().length);
+    cdsRef = cdsSeq.getDBRefs()[0];
     assertEquals("EMBLCDS", cdsRef.getSource());
     assertEquals("4", cdsRef.getVersion());
     assertEquals("A12347", cdsRef.getAccessionId());
 
     /*
-     * Verify there are mappings from each exon sequence to its protein product
+     * Verify there are mappings from each cds sequence to its protein product
      * and also to its dna source
      */
     Iterator<AlignedCodonFrame> newMappingsIterator = mappings.iterator();
 
     // mappings for dna1 - exon1 - pep1
-    AlignedCodonFrame exonMapping = newMappingsIterator.next();
-    List<Mapping> dnaMappings = exonMapping.getMappingsForSequence(dna1);
+    AlignedCodonFrame cdsMapping = newMappingsIterator.next();
+    List<Mapping> dnaMappings = cdsMapping.getMappingsForSequence(dna1);
     assertEquals(1, dnaMappings.size());
-    assertSame(exons.get(0).getDatasetSequence(), dnaMappings.get(0)
+    assertSame(cds.get(0).getDatasetSequence(), dnaMappings.get(0)
             .getTo());
     assertEquals("G(1) in CDS should map to G(4) in DNA", 4, dnaMappings
             .get(0).getMap().getToPosition(1));
-    List<Mapping> peptideMappings = exonMapping
+    List<Mapping> peptideMappings = cdsMapping
             .getMappingsForSequence(pep1);
     assertEquals(1, peptideMappings.size());
     assertSame(pep1.getDatasetSequence(), peptideMappings.get(0).getTo());
 
-    // mappings for dna1 - exon2 - pep2
-    exonMapping = newMappingsIterator.next();
-    dnaMappings = exonMapping.getMappingsForSequence(dna1);
+    // mappings for dna1 - cds2 - pep2
+    cdsMapping = newMappingsIterator.next();
+    dnaMappings = cdsMapping.getMappingsForSequence(dna1);
     assertEquals(1, dnaMappings.size());
-    assertSame(exons.get(1).getDatasetSequence(), dnaMappings.get(0)
+    assertSame(cds.get(1).getDatasetSequence(), dnaMappings.get(0)
             .getTo());
     assertEquals("c(4) in CDS should map to c(7) in DNA", 7, dnaMappings
             .get(0).getMap().getToPosition(4));
-    peptideMappings = exonMapping.getMappingsForSequence(pep2);
+    peptideMappings = cdsMapping.getMappingsForSequence(pep2);
     assertEquals(1, peptideMappings.size());
     assertSame(pep2.getDatasetSequence(), peptideMappings.get(0).getTo());
 
-    // mappings for dna1 - exon3 - pep3
-    exonMapping = newMappingsIterator.next();
-    dnaMappings = exonMapping.getMappingsForSequence(dna1);
+    // mappings for dna1 - cds3 - pep3
+    cdsMapping = newMappingsIterator.next();
+    dnaMappings = cdsMapping.getMappingsForSequence(dna1);
     assertEquals(1, dnaMappings.size());
-    assertSame(exons.get(2).getDatasetSequence(), dnaMappings.get(0)
+    assertSame(cds.get(2).getDatasetSequence(), dnaMappings.get(0)
             .getTo());
     assertEquals("T(4) in CDS should map to T(10) in DNA", 10, dnaMappings
             .get(0).getMap().getToPosition(4));
-    peptideMappings = exonMapping.getMappingsForSequence(pep3);
+    peptideMappings = cdsMapping.getMappingsForSequence(pep3);
     assertEquals(1, peptideMappings.size());
     assertSame(pep3.getDatasetSequence(), peptideMappings.get(0).getTo());
   }
@@ -1279,4 +1281,213 @@ public class AlignmentUtilsTests
     assertTrue(AlignmentUtils.isMappable(al1, al2));
     assertTrue(AlignmentUtils.isMappable(al2, al1));
   }
+
+  /**
+   * Test creating a mapping when the sequences involved do not start at residue
+   * 1
+   * 
+   * @throws IOException
+   */
+  @Test(groups = { "Functional" })
+  public void testMapProteinSequenceToCdna_forSubsequence()
+          throws IOException
+  {
+    SequenceI prot = new Sequence("UNIPROT|V12345", "E-I--Q", 10, 12);
+    prot.createDatasetSequence();
+
+    SequenceI dna = new Sequence("EMBL|A33333", "GAA--AT-C-CAG", 40, 48);
+    dna.createDatasetSequence();
+
+    MapList map = AlignmentUtils.mapProteinSequenceToCdna(prot, dna);
+    assertEquals(10, map.getToLowest());
+    assertEquals(12, map.getToHighest());
+    assertEquals(40, map.getFromLowest());
+    assertEquals(48, map.getFromHighest());
+  }
+
+  /**
+   * Test for the alignSequenceAs method where we have protein mapped to protein
+   */
+  @Test(groups = { "Functional" })
+  public void testAlignSequenceAs_mappedProteinProtein()
+  {
+  
+    SequenceI alignMe = new Sequence("Match", "MGAASEV");
+    alignMe.createDatasetSequence();
+    SequenceI alignFrom = new Sequence("Query", "LQTGYMGAASEVMFSPTRR");
+    alignFrom.createDatasetSequence();
+
+    AlignedCodonFrame acf = new AlignedCodonFrame();
+    // this is like a domain or motif match of part of a peptide sequence
+    MapList map = new MapList(new int[] { 6, 12 }, new int[] { 1, 7 }, 1, 1);
+    acf.addMap(alignFrom.getDatasetSequence(),
+            alignMe.getDatasetSequence(), map);
+    
+    AlignmentUtils.alignSequenceAs(alignMe, alignFrom, acf, "-", '-', true,
+            true);
+    assertEquals("-----MGAASEV-------", alignMe.getSequenceAsString());
+  }
+
+  /**
+   * Test for the alignSequenceAs method where there are trailing unmapped
+   * residues in the model sequence
+   */
+  @Test(groups = { "Functional" })
+  public void testAlignSequenceAs_withTrailingPeptide()
+  {
+    // map first 3 codons to KPF; G is a trailing unmapped residue
+    MapList map = new MapList(new int[] { 1, 9 }, new int[] { 1, 3 }, 3, 1);
+  
+    checkAlignSequenceAs("AAACCCTTT", "K-PFG", true, true, map,
+            "AAA---CCCTTT---");
+  }
+
+  /**
+   * Tests for transferring features between mapped sequences
+   */
+  @Test(groups = { "Functional" })
+  public void testTransferFeatures()
+  {
+    SequenceI dna = new Sequence("dna/20-34", "acgTAGcaaGCCcgt");
+    SequenceI cds = new Sequence("cds/10-15", "TAGGCC");
+
+    // no overlap
+    dna.addSequenceFeature(new SequenceFeature("type1", "desc1", 1, 2, 1f,
+            null));
+    // partial overlap - to [1, 1]
+    dna.addSequenceFeature(new SequenceFeature("type2", "desc2", 3, 4, 2f,
+            null));
+    // exact overlap - to [1, 3]
+    dna.addSequenceFeature(new SequenceFeature("type3", "desc3", 4, 6, 3f,
+            null));
+    // spanning overlap - to [2, 5]
+    dna.addSequenceFeature(new SequenceFeature("type4", "desc4", 5, 11, 4f,
+            null));
+    // exactly overlaps whole mapped range [1, 6]
+    dna.addSequenceFeature(new SequenceFeature("type5", "desc5", 4, 12, 5f,
+            null));
+    // no overlap (internal)
+    dna.addSequenceFeature(new SequenceFeature("type6", "desc6", 7, 9, 6f,
+            null));
+    // no overlap (3' end)
+    dna.addSequenceFeature(new SequenceFeature("type7", "desc7", 13, 15,
+            7f, null));
+    // overlap (3' end) - to [6, 6]
+    dna.addSequenceFeature(new SequenceFeature("type8", "desc8", 12, 12,
+            8f, null));
+    // extended overlap - to [6, +]
+    dna.addSequenceFeature(new SequenceFeature("type9", "desc9", 12, 13,
+            9f, null));
+
+    MapList map = new MapList(new int[] { 4, 6, 10, 12 },
+            new int[] { 1, 6 }, 1, 1);
+
+    /*
+     * transferFeatures() will build 'partial overlap' for regions
+     * that partially overlap 5' or 3' (start or end) of target sequence
+     */
+    AlignmentUtils.transferFeatures(dna, cds, map, null);
+    SequenceFeature[] sfs = cds.getSequenceFeatures();
+    assertEquals(6, sfs.length);
+
+    SequenceFeature sf = sfs[0];
+    assertEquals("type2", sf.getType());
+    assertEquals("desc2", sf.getDescription());
+    assertEquals(2f, sf.getScore());
+    assertEquals(1, sf.getBegin());
+    assertEquals(1, sf.getEnd());
+
+    sf = sfs[1];
+    assertEquals("type3", sf.getType());
+    assertEquals("desc3", sf.getDescription());
+    assertEquals(3f, sf.getScore());
+    assertEquals(1, sf.getBegin());
+    assertEquals(3, sf.getEnd());
+
+    sf = sfs[2];
+    assertEquals("type4", sf.getType());
+    assertEquals(2, sf.getBegin());
+    assertEquals(5, sf.getEnd());
+
+    sf = sfs[3];
+    assertEquals("type5", sf.getType());
+    assertEquals(1, sf.getBegin());
+    assertEquals(6, sf.getEnd());
+
+    sf = sfs[4];
+    assertEquals("type8", sf.getType());
+    assertEquals(6, sf.getBegin());
+    assertEquals(6, sf.getEnd());
+
+    sf = sfs[5];
+    assertEquals("type9", sf.getType());
+    assertEquals(6, sf.getBegin());
+    assertEquals(6, sf.getEnd());
+  }
+
+  /**
+   * Tests for transferring features between mapped sequences
+   */
+  @Test(groups = { "Functional" })
+  public void testTransferFeatures_withOmit()
+  {
+    SequenceI dna = new Sequence("dna/20-34", "acgTAGcaaGCCcgt");
+    SequenceI cds = new Sequence("cds/10-15", "TAGGCC");
+
+    MapList map = new MapList(new int[] { 4, 6, 10, 12 },
+            new int[] { 1, 6 }, 1, 1);
+  
+    // [5, 11] maps to [2, 5]
+    dna.addSequenceFeature(new SequenceFeature("type4", "desc4", 5, 11, 4f,
+            null));
+    // [4, 12] maps to [1, 6]
+    dna.addSequenceFeature(new SequenceFeature("type5", "desc5", 4, 12, 5f,
+            null));
+    // [12, 12] maps to [6, 6]
+    dna.addSequenceFeature(new SequenceFeature("type8", "desc8", 12, 12,
+            8f, null));
+  
+    // desc4 and desc8 are the 'omit these' varargs
+    AlignmentUtils.transferFeatures(dna, cds, map, null, "type4", "type8");
+    SequenceFeature[] sfs = cds.getSequenceFeatures();
+    assertEquals(1, sfs.length);
+  
+    SequenceFeature sf = sfs[0];
+    assertEquals("type5", sf.getType());
+    assertEquals(1, sf.getBegin());
+    assertEquals(6, sf.getEnd());
+  }
+
+  /**
+   * Tests for transferring features between mapped sequences
+   */
+  @Test(groups = { "Functional" })
+  public void testTransferFeatures_withSelect()
+  {
+    SequenceI dna = new Sequence("dna/20-34", "acgTAGcaaGCCcgt");
+    SequenceI cds = new Sequence("cds/10-15", "TAGGCC");
+  
+    MapList map = new MapList(new int[] { 4, 6, 10, 12 },
+            new int[] { 1, 6 }, 1, 1);
+  
+    // [5, 11] maps to [2, 5]
+    dna.addSequenceFeature(new SequenceFeature("type4", "desc4", 5, 11, 4f,
+            null));
+    // [4, 12] maps to [1, 6]
+    dna.addSequenceFeature(new SequenceFeature("type5", "desc5", 4, 12, 5f,
+            null));
+    // [12, 12] maps to [6, 6]
+    dna.addSequenceFeature(new SequenceFeature("type8", "desc8", 12, 12,
+            8f, null));
+  
+    // "type5" is the 'select this type' argument
+    AlignmentUtils.transferFeatures(dna, cds, map, "type5");
+    SequenceFeature[] sfs = cds.getSequenceFeatures();
+    assertEquals(1, sfs.length);
+  
+    SequenceFeature sf = sfs[0];
+    assertEquals("type5", sf.getType());
+    assertEquals(1, sf.getBegin());
+    assertEquals(6, sf.getEnd());
+  }
 }