record and use that information to construct a mapping between the
sequence and downloaded structure.</p>
<p>If, for some reason, no SIFTS mapping data exists, then Jalview
- will generate a mapping using its built-in Needleman and Wunsch
- global alignment algorithm. This method of mapping was used for all
- structures prior to version 2.10.
- <p>
- <strong>Controlling and troubleshooting SIFTS mappings</strong> <br />
+ will generate a mapping using the built-in Needleman and Wunsch
+ global alignment algorithm. This is how sequence-structure mappings
+ were created before version 2.10.</p>
+ <p><strong>Controlling and troubleshooting SIFTS mappings</strong> <br />
Configuration options controlling whether SIFTS mappings are used
can be found in the <strong>Tools → Preferences →
Structure tab</strong>, under 'Sequence ↔ Structure method'.<br /> <em>Note:</em>
<strong>Multi-Chain Mappings</strong> <br />SIFTS gives Jalview the
ability to display multi-chain mappings between UniProt sequences
and PDB structure data. This is important when working with
- multimeric proteins, since the biological unit will contain several
+ multimeric proteins, when the biological assembly can contain several
structures for the same protein sequence. Multi-chain mapping allows
all residues in a structure to be located in the alignment, and
also, when shading the structure by sequence colours, enables
conservation patterns between oligomer interfaces to be explored.
</p>
- <p>To see this in action, load uniprot sequence for FER1_MAIZE
- then veiw PDB structure for 3B2F, you will notice that mousing over
+ <p>To see this in action, Retrieve the UniProt sequence
+ FER1_MAIZE, and then view one of its structures: 3B2F. Mousing over
the sequence results to two positions being highlighted in the
- structure, also colouring the sequence transfers the color to all
+ structure, and colouring the alignment transfers the color to all
the mapped chains in the structure.</p>
<p>
<Strong>Viewing Mapping Output</Strong> <br /> The mapping provided
by the SIFTS record is accessible via <strong>File →
View mapping</strong> menu of the structure viewers. The screenshot below
- is the mapping output for the <Strong>{FER1_MAIZE ↔
- 3B2F}</Strong> example described above, and confirms that all two chains
- were mapped. The mapping method used can be seen within the area
- highlighted with red boarder.
+ shows the mapping created between UniProt sequence FER1_MAIZE and proteins in PDB 3B2F, which reports thattwo chains
+ were mapped. The mapping method is also reported (highlighted with red border).
<p>
 <img src="sifts_mapping_output.png" align="left"
alt="SIFTS mapping output" />
+ <br/>
<p>
<em>SIFTS Mapping integration was added in Jalview 2.10</em>
</p>
<ul>
<li><strong>More accurate structure mappings.</strong>
Jalview now utilises the PDBe's SIFTS database (at EMBL-EBI)
- to match structures to UniProt sequences, even for structures
- containing multiple copies of a sequence.</li>
+ to <a href="features/siftsmapping.html">match structures
+ to UniProt sequences</a>, even for structures containing
+ multiple copies of a sequence.</li>
<li><strong>Import structures as mmCIF</strong>. Jalview
now downloads data from the EMBL-EBI's PDBe site as mmCIF.
mmCIF files allow Jalview to handle very large structures,