On selecting protein cross-references (for a cDNA alignment), or DNA xrefs (for peptide), a Split Frame view is opened showing cDNA and peptide.</p>
<p><strong><em>Realign a Split View</em></strong></p>
-<p>If you invoke a web service to realign either half of a Split Frame, then the resulting realignment is displayed in a new
-Split Frame.</p>
-<ul>
-<li>the alignment you chose to realign (for example, peptide) is displayed as aligned by the external web service</li>
-<li>Jalview 'aligns' its complement (in this case, cDNA) similarly, by inserting corresponding gaps
- <ul>
- <li>NB this is <em>not</em> the same as aligning the complement using the external service, which may give different results</li>
- </ul>
-</li>
-</ul>
-
-<p><strong><em>Applet</em></strong></p>
-<p>To see a split frame view in the Jalview applet, provide these applet parameters:
-<table border="1">
-<tr><th>Parameter</th><th>Value</th><th>Description</th>
-<tr><td>file</td><td>path to an alignment file</td><td>a cDNA (or protein) alignment</td>
-<tr><td>file</td><td>path to an alignment file</td><td>a protein (or cDNA) alignment</td>
-<tr><td>enableSplitFrame</td><td>true</td><td>to enable the Split Frame feature</td>
-</table>
-<p/>If compatible sequences are present in the input alignments, Jalview will open a Split Frame view.<br/>
-If not, only the first alignment will be opened (an error message is written to the Java console).
-
- <p>
+ <p>If you invoke a web service to realign either half of a Split
+ Frame, then the resulting realignment is displayed in a new Split
+ Frame.
+ </p>
+ <ul>
+ <li>the alignment you chose to realign (for example, peptide)
+ is displayed as aligned by the external web service</li>
+ <li>Jalview reconstructs the alignment of its complement (in
+ this case, cDNA) by inserting gaps in the corresponding positions</li>
+ </ul>
+ <p><strong>Reconstructed Alignments</strong>
+ Reconstructed alignments are typically <em>not</em>
+ the same as the alignment produced by aligning the complement
+ sequence set directly with the external service. However, in the
+ case of protein alignments, a reconstructed cDNA alignment is often
+ more reliable than one calculated without coding information.
+ Reconstructed cDNA alignments are also more informative than the
+ original protein alignment when calculating phylogenetic trees or
+ performing other kinds of molecular evolution analysis.
+ </p>
+ <p>
<em>Split Frame Views were introduced in Jalview 2.9</em>
</p>
- <p>
- Example files for cDNA/Protein: <a
- href="http://www.jalview.org/builds/nextrel/examples/estrogenReceptorProtein.fa">estrogenReceptorProtein.fa</a>
- and <a
- href="http://www.jalview.org/builds/nextrel/examples/estrogenReceptorCdna.fa">estrogenReceptorCdna.fa</a>
- taken from xxx.
- </p>
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