Jalview</strong>
</p>
- <p>Predicted Alignment Error (PAE) matrices are produced by deep-learning
- based 3D-structure prediction pipelines such as AlphaFold. They reflect how reliably two parts of a model have been positioned in space, by giving for each residue the likely error (in Angstroms) between the modelled position and the pair of residues' real relative position.</p>
- <p>
- Jalview visualises PAE matrices as an alignment annotation track, shaded from dark green to white, as used on the EBI-AlphaFold website (see )
- </p>
+ <p>Predicted Alignment Error (PAE) matrices are produced by
+ deep-learning based 3D-structure prediction pipelines such as
+ AlphaFold. They reflect how reliably two parts of a model have been
+ positioned in space, by giving for each residue the likely error (in
+ Ångstroms) between that residue and every other modelled
+ position the pair of residues' real relative position, if the model
+ and real 3D structure were superimposed at that residue.</p>
+ <p>
+ Jalview visualises PAE matrices as an alignment annotation track,
+ shaded from dark green to white, similar to the encoding used on the
+ EBI-AlphaFold website (see <a
+ href="https://alphafold.ebi.ac.uk/entry/O04090">O04090 3D model</a>
+ at EBI-AlphaFoldDB).
+ </p>
<p>
<strong>Importing PAE Matrices</strong>
</p>
annotated with PAE matrices and PLDDT scores.
</p>
<p>
- See <a href="../features/paematrices.html">Working with PAE
- Matrices</a> for information on how they are visualised and analysed in
- Jalview.
- </p>
+ <strong>Showing PAE Matrix Annotations </strong>
</p>
<p>
- An additional <em>Predicted Alignment Error</em> file can also be
- provided when importing 3D structure data. Jalview supports import of
- PAE Matrices provided as <a href="../io/paematrixformat.html">AlphaFold
- format JSON files</a> - which are also produced by ColabFold. See <a
- href="paematrices.html">Working with PAE Matrices</a> for details on
- what Jalview allows you to do with associated PAE matrix data.
+ When viewing 3D structures from the EBI-AlphaFold database or local 3D
+ structures with an associated PAE file, the PAE is imported as <i>Reference
+ Annotation</i>, which is not always automatically added to the alignment
+ view.
</p>
+ <p>To show the PAE, right click the sequence and locate the 'Add
+ Reference Annotation' entry in the Sequence ID submenu, or select all
+ sequences and locate the option in the Selection submenu. You can do
+ this in any alignment window (or view) where a sequence with
+ associated PAE data appears.</p>
+ <p></p>
<p>
- <em>The Structure Chooser interface was introduced in Jalview
- 2.9. </em>
+ <em>Support for visualision and analysis of predicted alignment
+ error matrices was added in Jalview 2.11.3. </em>
</p>
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