Jalview</strong>
</p>
- <p>Predicted Alignment Error matrices are produced by deep-learning
- based 3D-structure prediction pipelines such as AlphaFold.</p>
+ <p>Predicted Alignment Error (PAE) matrices are produced by deep-learning
+ based 3D-structure prediction pipelines such as AlphaFold. They reflect how reliably two parts of a model have been positioned in space, by giving for each residue the likely error (in Angstroms) between the modelled position and the pair of residues' real relative position.</p>
+ <p>
+ Jalview visualises PAE matrices as an alignment annotation track, shaded from dark green to white, as used on the EBI-AlphaFold website (see )
+ </p>
+ <p>
+ <strong>Importing PAE Matrices</strong>
+ </p>
<p>
Jalview retrieves PAE matrices when importing predicted 3D structures
from the EBI-AlphaFold database via <a
href="../features/structurechooser.html">Jalview's structure
- chooser</a> GUI.
+ chooser</a> GUI. If you have produced your own models and accompanying
+ PAE matrices using a pipeline such as ColabFold, then you can load
+ them both together via the <a
+ href="../features/structurechooser.html#loadpdbfile">Load PDB
+ File</a> dropdown menu in the 3D structure chooser. in a <a
+ href="../io/paematrixformat.html">supported PAE format</a>.
+ </p>
+ <p>
+ The <a href="../features/clarguments-basic.html">Command Line
+ Interface</a> also provides a options for importing PAE matrices along
+ side models, enabling the automated production of alignment figures
+ annotated with PAE matrices and PLDDT scores.
</p>
<p>
- If you have produced your own models using a pipeline such as
- ColabFold, then you can load it via the 'Add PAE matrix file' button
- in the <a href="../features/structurechooser.html#loadpdbfile">Load
- PDB File</a> dropdown menu in the 3D structure chooser, providing it is
- in a <a href="../io/paematrixformat.html">supported PAE format</a>. ,
- or the <a href="../features/clarguments-basic.html">Command Line
- Interface</a>. See <a href="../features/paematrices.html">Working
- with PAE Matrices</a> for information on how they are visualised and
- analysed in Jalview.
+ See <a href="../features/paematrices.html">Working with PAE
+ Matrices</a> for information on how they are visualised and analysed in
+ Jalview.
</p>
</p>
<p>