<strong>Alignment RNA Structure Consensus Annotation</strong>
</p>
- <p>
- The RNA structure consensus displayed below the alignment is the
- percentage of valid base pairs per column. It is calculated in
- relation to a secondary structure and just paired columns are
- calculated. The canonical Watson-Crick base pairings (A-T/U, G-C)
- and the wobble base pair (G-T/U) are regarded as valid pairings.<br>
- The amount of valid base pairs is indicated by the profile in the
- Alignment Annotation row.<br> By default this calculation
- includes gaps in columns. You can choose to ignore gaps in the
- calculation by right clicking on the label "StrConsensus"
- to the left of the structure consensus bar chart.<br>
+ <p>The RNA structure consensus displayed below the alignment gives
+ the percentage of valid base pairs per column for the first
+ secondary structure annotation shown on the annotation panel. The
+ symbol below each histogram indicates whether the majority of base
+ pairs are:
+ <ul>
+ <li>'(' - Watson-Crick (A:C, G:T)</li>
+ <li>'[' - Canonical Pairs (A:C, G:U, A:U)</li>
+ <li>'{' - Invalid Pairs (the rest)</li>
+ </ul>
+ <p>Mousing over the column gives the fraction of pairs classified as Watson-Crick, Canonical or Invalid.</p>
+
+<p>By default
+ this calculation includes gaps in columns. You can choose to ignore
+ gaps in the calculation by right clicking on the label
+ "StrConsensus" to the left of the structure consensus bar
+ chart.<br>
<p>
<strong>Structure logo</strong>
</p>
- By clicking on the label you can also activate the structure logo. It
- is very similar to a sequence logo but counts the numbers of base
- pairs. There are two residues per column, the actual column and the
- interacting base. The opening bracket is always the one on the left
- side.
- <br> Like sequence logos the relative amount of a specific base
- pair can be estimated by its size in the logo. The tool tip of a
- column gives the exact numbers for all occurring valid base pairs.
+ Right-clicking on the label allows you to enable the structure logo.
+ It is very similar to a sequence logo but instead shows the
+ distribution of base pairs. There are two residues per column, the
+ actual column and the interacting base. The opening bracket is always
+ the one on the left side.
+ <br> Like sequence logos the relative amount of a specific base pair
+ can be estimated by its size in the logo. When the logo is displayed,
+ the tool tip of a column gives the exact numbers for all occurring
+ valid base pairs.
</p>
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