*/
package jalview.analysis;
+import java.util.ArrayList;
+import java.util.Arrays;
+import java.util.Collection;
+import java.util.Collections;
+import java.util.HashMap;
+import java.util.HashSet;
+import java.util.Iterator;
+import java.util.LinkedHashMap;
+import java.util.List;
+import java.util.Map;
+import java.util.Map.Entry;
+import java.util.NoSuchElementException;
+import java.util.Set;
+import java.util.SortedMap;
+import java.util.TreeMap;
+
+import jalview.bin.Cache;
+import jalview.commands.RemoveGapColCommand;
import jalview.datamodel.AlignedCodon;
import jalview.datamodel.AlignedCodonFrame;
+import jalview.datamodel.AlignedCodonFrame.SequenceToSequenceMapping;
import jalview.datamodel.Alignment;
import jalview.datamodel.AlignmentAnnotation;
import jalview.datamodel.AlignmentI;
import jalview.datamodel.DBRefEntry;
-import jalview.datamodel.DBRefSource;
-import jalview.datamodel.FeatureProperties;
+import jalview.datamodel.GeneLociI;
import jalview.datamodel.IncompleteCodonException;
import jalview.datamodel.Mapping;
-import jalview.datamodel.SearchResults;
import jalview.datamodel.Sequence;
import jalview.datamodel.SequenceFeature;
import jalview.datamodel.SequenceGroup;
import jalview.datamodel.SequenceI;
-import jalview.io.gff.SequenceOntologyFactory;
+import jalview.datamodel.features.SequenceFeatures;
import jalview.io.gff.SequenceOntologyI;
import jalview.schemes.ResidueProperties;
import jalview.util.Comparison;
import jalview.util.DBRefUtils;
+import jalview.util.IntRangeComparator;
import jalview.util.MapList;
import jalview.util.MappingUtils;
-import java.util.ArrayList;
-import java.util.Arrays;
-import java.util.Collection;
-import java.util.Collections;
-import java.util.Comparator;
-import java.util.HashMap;
-import java.util.HashSet;
-import java.util.Iterator;
-import java.util.LinkedHashMap;
-import java.util.List;
-import java.util.Map;
-import java.util.Map.Entry;
-import java.util.Set;
-import java.util.TreeMap;
-
/**
* grab bag of useful alignment manipulation operations Expect these to be
* refactored elsewhere at some point.
*/
public class AlignmentUtils
{
+ private static final int CODON_LENGTH = 3;
+
+ private static final String SEQUENCE_VARIANT = "sequence_variant:";
+
+ /*
+ * the 'id' attribute is provided for variant features fetched from
+ * Ensembl using its REST service with JSON format
+ */
+ public static final String VARIANT_ID = "id";
+
+ /**
+ * A data model to hold the 'normal' base value at a position, and an optional
+ * sequence variant feature
+ */
+ static final class DnaVariant
+ {
+ final String base;
+
+ SequenceFeature variant;
+
+ DnaVariant(String nuc)
+ {
+ base = nuc;
+ variant = null;
+ }
+
+ DnaVariant(String nuc, SequenceFeature var)
+ {
+ base = nuc;
+ variant = var;
+ }
+
+ public String getSource()
+ {
+ return variant == null ? null : variant.getFeatureGroup();
+ }
+
+ /**
+ * toString for aid in the debugger only
+ */
+ @Override
+ public String toString()
+ {
+ return base + ":" + (variant == null ? "" : variant.getDescription());
+ }
+ }
/**
* given an existing alignment, create a new alignment including all, or up to
*/
public static AlignmentI expandContext(AlignmentI core, int flankSize)
{
- List<SequenceI> sq = new ArrayList<SequenceI>();
+ List<SequenceI> sq = new ArrayList<>();
int maxoffset = 0;
for (SequenceI s : core.getSequences())
{
}
}
// TODO use Character.toLowerCase to avoid creating String objects?
- char[] upstream = new String(ds.getSequence(s.getStart() - 1
- - ustream_ds, s.getStart() - 1)).toLowerCase().toCharArray();
- char[] downstream = new String(ds.getSequence(s_end - 1, s_end
- + dstream_ds)).toLowerCase().toCharArray();
+ char[] upstream = new String(ds
+ .getSequence(s.getStart() - 1 - ustream_ds, s.getStart() - 1))
+ .toLowerCase().toCharArray();
+ char[] downstream = new String(
+ ds.getSequence(s_end - 1, s_end + dstream_ds)).toLowerCase()
+ .toCharArray();
char[] coreseq = s.getSequence();
char[] nseq = new char[offset + upstream.length + downstream.length
+ coreseq.length];
System.arraycopy(upstream, 0, nseq, p, upstream.length);
System.arraycopy(coreseq, 0, nseq, p + upstream.length,
coreseq.length);
- System.arraycopy(downstream, 0, nseq, p + coreseq.length
- + upstream.length, downstream.length);
+ System.arraycopy(downstream, 0, nseq,
+ p + coreseq.length + upstream.length, downstream.length);
s.setSequence(new String(nseq));
s.setStart(s.getStart() - ustream_ds);
s.setEnd(s_end + downstream.length);
public static Map<String, List<SequenceI>> getSequencesByName(
AlignmentI al)
{
- Map<String, List<SequenceI>> theMap = new LinkedHashMap<String, List<SequenceI>>();
+ Map<String, List<SequenceI>> theMap = new LinkedHashMap<>();
for (SequenceI seq : al.getSequences())
{
String name = seq.getName();
List<SequenceI> seqs = theMap.get(name);
if (seqs == null)
{
- seqs = new ArrayList<SequenceI>();
+ seqs = new ArrayList<>();
theMap.put(name, seqs);
}
seqs.add(seq);
return false;
}
- Set<SequenceI> mappedDna = new HashSet<SequenceI>();
- Set<SequenceI> mappedProtein = new HashSet<SequenceI>();
+ Set<SequenceI> mappedDna = new HashSet<>();
+ Set<SequenceI> mappedProtein = new HashSet<>();
/*
* First pass - map sequences where cross-references exist. This include
* @return
*/
protected static boolean mapProteinToCdna(
- final AlignmentI proteinAlignment,
- final AlignmentI cdnaAlignment, Set<SequenceI> mappedDna,
- Set<SequenceI> mappedProtein, boolean xrefsOnly)
+ final AlignmentI proteinAlignment, final AlignmentI cdnaAlignment,
+ Set<SequenceI> mappedDna, Set<SequenceI> mappedProtein,
+ boolean xrefsOnly)
{
boolean mappingExistsOrAdded = false;
List<SequenceI> thisSeqs = proteinAlignment.getSequences();
* Don't map non-xrefd sequences more than once each. This heuristic
* allows us to pair up similar sequences in ordered alignments.
*/
- if (!xrefsOnly
- && (mappedProtein.contains(aaSeq) || mappedDna
- .contains(cdnaSeq)))
+ if (!xrefsOnly && (mappedProtein.contains(aaSeq)
+ || mappedDna.contains(cdnaSeq)))
{
continue;
}
}
else
{
- MapList map = mapProteinSequenceToCdna(aaSeq, cdnaSeq);
+ MapList map = mapCdnaToProtein(aaSeq, cdnaSeq);
if (map != null)
{
acf.addMap(cdnaSeq, aaSeq, map);
* Answers true if the mappings include one between the given (dataset)
* sequences.
*/
- public static boolean mappingExists(List<AlignedCodonFrame> mappings,
+ protected static boolean mappingExists(List<AlignedCodonFrame> mappings,
SequenceI aaSeq, SequenceI cdnaSeq)
{
if (mappings != null)
}
/**
- * Build a mapping (if possible) of a protein to a cDNA sequence. The cDNA
- * must be three times the length of the protein, possibly after ignoring
- * start and/or stop codons, and must translate to the protein. Returns null
- * if no mapping is determined.
+ * Builds a mapping (if possible) of a cDNA to a protein sequence.
+ * <ul>
+ * <li>first checks if the cdna translates exactly to the protein
+ * sequence</li>
+ * <li>else checks for translation after removing a STOP codon</li>
+ * <li>else checks for translation after removing a START codon</li>
+ * <li>if that fails, inspect CDS features on the cDNA sequence</li>
+ * </ul>
+ * Returns null if no mapping is determined.
*
- * @param proteinSeqs
+ * @param proteinSeq
+ * the aligned protein sequence
* @param cdnaSeq
+ * the aligned cdna sequence
* @return
*/
- public static MapList mapProteinSequenceToCdna(SequenceI proteinSeq,
+ public static MapList mapCdnaToProtein(SequenceI proteinSeq,
SequenceI cdnaSeq)
{
/*
* String objects.
*/
final SequenceI proteinDataset = proteinSeq.getDatasetSequence();
- char[] aaSeqChars = proteinDataset != null ? proteinDataset
- .getSequence() : proteinSeq.getSequence();
+ char[] aaSeqChars = proteinDataset != null
+ ? proteinDataset.getSequence()
+ : proteinSeq.getSequence();
final SequenceI cdnaDataset = cdnaSeq.getDatasetSequence();
char[] cdnaSeqChars = cdnaDataset != null ? cdnaDataset.getSequence()
: cdnaSeq.getSequence();
/*
* cdnaStart/End, proteinStartEnd are base 1 (for dataset sequence mapping)
*/
- final int mappedLength = 3 * aaSeqChars.length;
+ final int mappedLength = CODON_LENGTH * aaSeqChars.length;
int cdnaLength = cdnaSeqChars.length;
int cdnaStart = cdnaSeq.getStart();
int cdnaEnd = cdnaSeq.getEnd();
final int proteinEnd = proteinSeq.getEnd();
/*
- * If lengths don't match, try ignoring stop codon.
+ * If lengths don't match, try ignoring stop codon (if present)
*/
if (cdnaLength != mappedLength && cdnaLength > 2)
{
- String lastCodon = String.valueOf(cdnaSeqChars, cdnaLength - 3, 3)
- .toUpperCase();
- for (String stop : ResidueProperties.STOP)
+ String lastCodon = String.valueOf(cdnaSeqChars,
+ cdnaLength - CODON_LENGTH, CODON_LENGTH).toUpperCase();
+ for (String stop : ResidueProperties.STOP_CODONS)
{
if (lastCodon.equals(stop))
{
- cdnaEnd -= 3;
- cdnaLength -= 3;
+ cdnaEnd -= CODON_LENGTH;
+ cdnaLength -= CODON_LENGTH;
break;
}
}
* If lengths still don't match, try ignoring start codon.
*/
int startOffset = 0;
- if (cdnaLength != mappedLength
- && cdnaLength > 2
- && String.valueOf(cdnaSeqChars, 0, 3).toUpperCase()
+ if (cdnaLength != mappedLength && cdnaLength > 2
+ && String.valueOf(cdnaSeqChars, 0, CODON_LENGTH).toUpperCase()
.equals(ResidueProperties.START))
{
- startOffset += 3;
- cdnaStart += 3;
- cdnaLength -= 3;
+ startOffset += CODON_LENGTH;
+ cdnaStart += CODON_LENGTH;
+ cdnaLength -= CODON_LENGTH;
}
- if (cdnaLength != mappedLength)
- {
- return null;
- }
- if (!translatesAs(cdnaSeqChars, startOffset, aaSeqChars))
+ if (translatesAs(cdnaSeqChars, startOffset, aaSeqChars))
{
- return null;
+ /*
+ * protein is translation of dna (+/- start/stop codons)
+ */
+ MapList map = new MapList(new int[] { cdnaStart, cdnaEnd },
+ new int[]
+ { proteinStart, proteinEnd }, CODON_LENGTH, 1);
+ return map;
}
- MapList map = new MapList(new int[] { cdnaStart, cdnaEnd }, new int[] {
- proteinStart, proteinEnd }, 3, 1);
- return map;
+
+ /*
+ * translation failed - try mapping CDS annotated regions of dna
+ */
+ return mapCdsToProtein(cdnaSeq, proteinSeq);
}
/**
return false;
}
- int aaResidue = 0;
- for (int i = cdnaStart; i < cdnaSeqChars.length - 2
- && aaResidue < aaSeqChars.length; i += 3, aaResidue++)
+ int aaPos = 0;
+ int dnaPos = cdnaStart;
+ for (; dnaPos < cdnaSeqChars.length - 2
+ && aaPos < aaSeqChars.length; dnaPos += CODON_LENGTH, aaPos++)
{
- String codon = String.valueOf(cdnaSeqChars, i, 3);
+ String codon = String.valueOf(cdnaSeqChars, dnaPos, CODON_LENGTH);
final String translated = ResidueProperties.codonTranslate(codon);
+
/*
* allow * in protein to match untranslatable in dna
*/
- final char aaRes = aaSeqChars[aaResidue];
- if ((translated == null || "STOP".equals(translated)) && aaRes == '*')
+ final char aaRes = aaSeqChars[aaPos];
+ if ((translated == null || ResidueProperties.STOP.equals(translated))
+ && aaRes == '*')
{
continue;
}
return false;
}
}
- // fail if we didn't match all of the aa sequence
- return (aaResidue == aaSeqChars.length);
+
+ /*
+ * check we matched all of the protein sequence
+ */
+ if (aaPos != aaSeqChars.length)
+ {
+ return false;
+ }
+
+ /*
+ * check we matched all of the dna except
+ * for optional trailing STOP codon
+ */
+ if (dnaPos == cdnaSeqChars.length)
+ {
+ return true;
+ }
+ if (dnaPos == cdnaSeqChars.length - CODON_LENGTH)
+ {
+ String codon = String.valueOf(cdnaSeqChars, dnaPos, CODON_LENGTH);
+ if (ResidueProperties.STOP
+ .equals(ResidueProperties.codonTranslate(codon)))
+ {
+ return true;
+ }
+ }
+ return false;
}
/**
AlignedCodonFrame mapping = null;
for (AlignedCodonFrame mp : mappings)
{
- alignFrom = mp.findAlignedSequence(seq.getDatasetSequence(), al);
+ alignFrom = mp.findAlignedSequence(seq, al);
if (alignFrom != null)
{
mapping = mp;
* @param preserveUnmappedGaps
* @param preserveMappedGaps
*/
- public static void alignSequenceAs(SequenceI alignTo,
- SequenceI alignFrom, AlignedCodonFrame mapping, String myGap,
- char sourceGap, boolean preserveMappedGaps,
- boolean preserveUnmappedGaps)
+ public static void alignSequenceAs(SequenceI alignTo, SequenceI alignFrom,
+ AlignedCodonFrame mapping, String myGap, char sourceGap,
+ boolean preserveMappedGaps, boolean preserveUnmappedGaps)
{
// TODO generalise to work for Protein-Protein, dna-dna, dna-protein
int toOffset = alignTo.getStart() - 1;
int sourceGapMappedLength = 0;
boolean inExon = false;
- final char[] thisSeq = alignTo.getSequence();
- final char[] thatAligned = alignFrom.getSequence();
- StringBuilder thisAligned = new StringBuilder(2 * thisSeq.length);
+ final int toLength = alignTo.getLength();
+ final int fromLength = alignFrom.getLength();
+ StringBuilder thisAligned = new StringBuilder(2 * toLength);
/*
* Traverse the 'model' aligned sequence
*/
- for (char sourceChar : thatAligned)
+ for (int i = 0; i < fromLength; i++)
{
+ char sourceChar = alignFrom.getCharAt(i);
if (sourceChar == sourceGap)
{
sourceGapMappedLength += ratio;
*/
int intronLength = 0;
while (basesWritten + toOffset < mappedCodonEnd
- && thisSeqPos < thisSeq.length)
+ && thisSeqPos < toLength)
{
- final char c = thisSeq[thisSeqPos++];
+ final char c = alignTo.getCharAt(thisSeqPos++);
if (c != myGapChar)
{
basesWritten++;
int gapsToAdd = calculateGapsToInsert(preserveMappedGaps,
preserveUnmappedGaps, sourceGapMappedLength, inExon,
trailingCopiedGap.length(), intronLength, startOfCodon);
- for (int i = 0; i < gapsToAdd; i++)
+ for (int k = 0; k < gapsToAdd; k++)
{
thisAligned.append(myGapChar);
}
* At end of model aligned sequence. Copy any remaining target sequence, optionally
* including (intron) gaps.
*/
- while (thisSeqPos < thisSeq.length)
+ while (thisSeqPos < toLength)
{
- final char c = thisSeq[thisSeqPos++];
+ final char c = alignTo.getCharAt(thisSeqPos++);
if (c != myGapChar || preserveUnmappedGaps)
{
thisAligned.append(c);
}
else
{
- gapsToAdd = Math.min(intronLength + trailingGapLength
- - sourceGapMappedLength, trailingGapLength);
+ gapsToAdd = Math.min(
+ intronLength + trailingGapLength - sourceGapMappedLength,
+ trailingGapLength);
}
}
}
}
/**
- * Returns a list of sequences mapped from the given sequences and aligned
- * (gapped) in the same way. For example, the cDNA for aligned protein, where
- * a single gap in protein generates three gaps in cDNA.
+ * Realigns the given protein to match the alignment of the dna, using codon
+ * mappings to translate aligned codon positions to protein residues.
*
- * @param sequences
- * @param gapCharacter
- * @param mappings
- * @return
+ * @param protein
+ * the alignment whose sequences are realigned by this method
+ * @param dna
+ * the dna alignment whose alignment we are 'copying'
+ * @return the number of sequences that were realigned
*/
- public static List<SequenceI> getAlignedTranslation(
- List<SequenceI> sequences, char gapCharacter,
- Set<AlignedCodonFrame> mappings)
+ public static int alignProteinAsDna(AlignmentI protein, AlignmentI dna)
{
- List<SequenceI> alignedSeqs = new ArrayList<SequenceI>();
-
- for (SequenceI seq : sequences)
+ if (protein.isNucleotide() || !dna.isNucleotide())
{
- List<SequenceI> mapped = getAlignedTranslation(seq, gapCharacter,
- mappings);
- alignedSeqs.addAll(mapped);
+ System.err.println("Wrong alignment type in alignProteinAsDna");
+ return 0;
}
- return alignedSeqs;
+ List<SequenceI> unmappedProtein = new ArrayList<>();
+ Map<AlignedCodon, Map<SequenceI, AlignedCodon>> alignedCodons = buildCodonColumnsMap(
+ protein, dna, unmappedProtein);
+ return alignProteinAs(protein, alignedCodons, unmappedProtein);
}
/**
- * Returns sequences aligned 'like' the source sequence, as mapped by the
- * given mappings. Normally we expect zero or one 'mapped' sequences, but this
- * will support 1-to-many as well.
+ * Realigns the given dna to match the alignment of the protein, using codon
+ * mappings to translate aligned peptide positions to codons.
*
- * @param seq
- * @param gapCharacter
- * @param mappings
- * @return
+ * Always produces a padded CDS alignment.
+ *
+ * @param dna
+ * the alignment whose sequences are realigned by this method
+ * @param protein
+ * the protein alignment whose alignment we are 'copying'
+ * @return the number of sequences that were realigned
*/
- protected static List<SequenceI> getAlignedTranslation(SequenceI seq,
- char gapCharacter, Set<AlignedCodonFrame> mappings)
+ public static int alignCdsAsProtein(AlignmentI dna, AlignmentI protein)
{
- List<SequenceI> result = new ArrayList<SequenceI>();
- for (AlignedCodonFrame mapping : mappings)
+ if (protein.isNucleotide() || !dna.isNucleotide())
+ {
+ System.err.println("Wrong alignment type in alignProteinAsDna");
+ return 0;
+ }
+ // todo: implement this
+ List<AlignedCodonFrame> mappings = protein.getCodonFrames();
+ int alignedCount = 0;
+ int width = 0; // alignment width for padding CDS
+ for (SequenceI dnaSeq : dna.getSequences())
{
- if (mapping.involvesSequence(seq))
+ if (alignCdsSequenceAsProtein(dnaSeq, protein, mappings,
+ dna.getGapCharacter()))
{
- SequenceI mapped = getAlignedTranslation(seq, gapCharacter, mapping);
- if (mapped != null)
- {
- result.add(mapped);
- }
+ alignedCount++;
}
+ width = Math.max(dnaSeq.getLength(), width);
}
- return result;
+ int oldwidth;
+ int diff;
+ for (SequenceI dnaSeq : dna.getSequences())
+ {
+ oldwidth = dnaSeq.getLength();
+ diff = width - oldwidth;
+ if (diff > 0)
+ {
+ dnaSeq.insertCharAt(oldwidth, diff, dna.getGapCharacter());
+ }
+ }
+ return alignedCount;
}
/**
- * Returns the translation of 'seq' (as held in the mapping) with
- * corresponding alignment (gaps).
+ * Helper method to align (if possible) the dna sequence to match the
+ * alignment of a mapped protein sequence. This is currently limited to
+ * handling coding sequence only.
*
- * @param seq
- * @param gapCharacter
- * @param mapping
+ * @param cdsSeq
+ * @param protein
+ * @param mappings
+ * @param gapChar
* @return
*/
- protected static SequenceI getAlignedTranslation(SequenceI seq,
- char gapCharacter, AlignedCodonFrame mapping)
+ static boolean alignCdsSequenceAsProtein(SequenceI cdsSeq,
+ AlignmentI protein, List<AlignedCodonFrame> mappings,
+ char gapChar)
{
- String gap = String.valueOf(gapCharacter);
- boolean toDna = false;
- int fromRatio = 1;
- SequenceI mapTo = mapping.getDnaForAaSeq(seq);
- if (mapTo != null)
- {
- // mapping is from protein to nucleotide
- toDna = true;
- // should ideally get gap count ratio from mapping
- gap = String.valueOf(new char[] { gapCharacter, gapCharacter,
- gapCharacter });
- }
- else
+ SequenceI cdsDss = cdsSeq.getDatasetSequence();
+ if (cdsDss == null)
{
- // mapping is from nucleotide to protein
- mapTo = mapping.getAaForDnaSeq(seq);
- fromRatio = 3;
+ System.err
+ .println("alignCdsSequenceAsProtein needs aligned sequence!");
+ return false;
}
- StringBuilder newseq = new StringBuilder(seq.getLength()
- * (toDna ? 3 : 1));
- int residueNo = 0; // in seq, base 1
- int[] phrase = new int[fromRatio];
- int phraseOffset = 0;
- int gapWidth = 0;
- boolean first = true;
- final Sequence alignedSeq = new Sequence("", "");
-
- for (char c : seq.getSequence())
+ List<AlignedCodonFrame> dnaMappings = MappingUtils
+ .findMappingsForSequence(cdsSeq, mappings);
+ for (AlignedCodonFrame mapping : dnaMappings)
{
- if (c == gapCharacter)
- {
- gapWidth++;
- if (gapWidth >= fromRatio)
- {
- newseq.append(gap);
- gapWidth = 0;
- }
- }
- else
+ List<SequenceToSequenceMapping> foundMap=new ArrayList<>();
+ SequenceI peptide = mapping.findAlignedSequence(cdsSeq, protein,foundMap);
+ if (peptide != null)
{
- phrase[phraseOffset++] = residueNo + 1;
- if (phraseOffset == fromRatio)
+ final int peptideLength = peptide.getLength();
+ Mapping map = foundMap.get(0).getMapping();
+ if (map != null)
{
+ MapList mapList = map.getMap();
+ if (map.getTo() == peptide.getDatasetSequence())
+ {
+ mapList = mapList.getInverse();
+ }
+ final int cdsLength = cdsDss.getLength();
+ int mappedFromLength = MappingUtils.getLength(mapList
+ .getFromRanges());
+ int mappedToLength = MappingUtils
+ .getLength(mapList.getToRanges());
+ boolean addStopCodon = (cdsLength == mappedFromLength
+ * CODON_LENGTH + CODON_LENGTH)
+ || (peptide.getDatasetSequence()
+ .getLength() == mappedFromLength - 1);
+ if (cdsLength != mappedToLength && !addStopCodon)
+ {
+ System.err.println(String.format(
+ "Can't align cds as protein (length mismatch %d/%d): %s",
+ cdsLength, mappedToLength, cdsSeq.getName()));
+ }
+
+ /*
+ * pre-fill the aligned cds sequence with gaps
+ */
+ char[] alignedCds = new char[peptideLength * CODON_LENGTH
+ + (addStopCodon ? CODON_LENGTH : 0)];
+ Arrays.fill(alignedCds, gapChar);
+
/*
- * Have read a whole codon (or protein residue), now translate: map
- * source phrase to positions in target sequence add characters at
- * these positions to newseq Note mapping positions are base 1, our
- * sequence positions base 0.
+ * walk over the aligned peptide sequence and insert mapped
+ * codons for residues in the aligned cds sequence
*/
- SearchResults sr = new SearchResults();
- for (int pos : phrase)
+ int copiedBases = 0;
+ int cdsStart = cdsDss.getStart();
+ int proteinPos = peptide.getStart() - 1;
+ int cdsCol = 0;
+
+ for (int col = 0; col < peptideLength; col++)
{
- mapping.markMappedRegion(seq, pos, sr);
+ char residue = peptide.getCharAt(col);
+
+ if (Comparison.isGap(residue))
+ {
+ cdsCol += CODON_LENGTH;
+ }
+ else
+ {
+ proteinPos++;
+ int[] codon = mapList.locateInTo(proteinPos, proteinPos);
+ if (codon == null)
+ {
+ // e.g. incomplete start codon, X in peptide
+ cdsCol += CODON_LENGTH;
+ }
+ else
+ {
+ for (int j = codon[0]; j <= codon[1]; j++)
+ {
+ char mappedBase = cdsDss.getCharAt(j - cdsStart);
+ alignedCds[cdsCol++] = mappedBase;
+ copiedBases++;
+ }
+ }
+ }
}
- newseq.append(sr.getCharacters());
- if (first)
+
+ /*
+ * append stop codon if not mapped from protein,
+ * closing it up to the end of the mapped sequence
+ */
+ if (copiedBases == cdsLength - CODON_LENGTH)
{
- first = false;
- // Hack: Copy sequence dataset, name and description from
- // SearchResults.match[0].sequence
- // TODO? carry over sequence names from original 'complement'
- // alignment
- SequenceI mappedTo = sr.getResultSequence(0);
- alignedSeq.setName(mappedTo.getName());
- alignedSeq.setDescription(mappedTo.getDescription());
- alignedSeq.setDatasetSequence(mappedTo);
+ for (int i = alignedCds.length - 1; i >= 0; i--)
+ {
+ if (!Comparison.isGap(alignedCds[i]))
+ {
+ cdsCol = i + 1; // gap just after end of sequence
+ break;
+ }
+ }
+ for (int i = cdsLength - CODON_LENGTH; i < cdsLength; i++)
+ {
+ alignedCds[cdsCol++] = cdsDss.getCharAt(i);
+ }
}
- phraseOffset = 0;
+ cdsSeq.setSequence(new String(alignedCds));
+ return true;
}
- residueNo++;
}
}
- alignedSeq.setSequence(newseq.toString());
- return alignedSeq;
+ return false;
}
/**
- * Realigns the given protein to match the alignment of the dna, using codon
- * mappings to translate aligned codon positions to protein residues.
+ * Builds a map whose key is an aligned codon position (3 alignment column
+ * numbers base 0), and whose value is a map from protein sequence to each
+ * protein's peptide residue for that codon. The map generates an ordering of
+ * the codons, and allows us to read off the peptides at each position in
+ * order to assemble 'aligned' protein sequences.
*
* @param protein
- * the alignment whose sequences are realigned by this method
+ * the protein alignment
* @param dna
- * the dna alignment whose alignment we are 'copying'
- * @return the number of sequences that were realigned
+ * the coding dna alignment
+ * @param unmappedProtein
+ * any unmapped proteins are added to this list
+ * @return
*/
- public static int alignProteinAsDna(AlignmentI protein, AlignmentI dna)
+ protected static Map<AlignedCodon, Map<SequenceI, AlignedCodon>> buildCodonColumnsMap(
+ AlignmentI protein, AlignmentI dna,
+ List<SequenceI> unmappedProtein)
{
- List<SequenceI> unmappedProtein = new ArrayList<SequenceI>();
+ /*
+ * maintain a list of any proteins with no mappings - these will be
+ * rendered 'as is' in the protein alignment as we can't align them
+ */
unmappedProtein.addAll(protein.getSequences());
List<AlignedCodonFrame> mappings = protein.getCodonFrames();
* {dnaSequence, {proteinSequence, codonProduct}} at that position. The
* comparator keeps the codon positions ordered.
*/
- Map<AlignedCodon, Map<SequenceI, String>> alignedCodons = new TreeMap<AlignedCodon, Map<SequenceI, String>>(
+ Map<AlignedCodon, Map<SequenceI, AlignedCodon>> alignedCodons = new TreeMap<>(
new CodonComparator());
+
for (SequenceI dnaSeq : dna.getSequences())
{
for (AlignedCodonFrame mapping : mappings)
{
- Mapping seqMap = mapping.getMappingForSequence(dnaSeq);
- SequenceI prot = mapping.findAlignedSequence(
- dnaSeq.getDatasetSequence(), protein);
+ SequenceI prot = mapping.findAlignedSequence(dnaSeq, protein);
if (prot != null)
{
- addCodonPositions(dnaSeq, prot, protein.getGapCharacter(),
- seqMap, alignedCodons);
+ Mapping seqMap = mapping.getMappingForSequence(dnaSeq);
+ addCodonPositions(dnaSeq, prot, protein.getGapCharacter(), seqMap,
+ alignedCodons);
unmappedProtein.remove(prot);
}
}
}
- return alignProteinAs(protein, alignedCodons, unmappedProtein);
+
+ /*
+ * Finally add any unmapped peptide start residues (e.g. for incomplete
+ * codons) as if at the codon position before the second residue
+ */
+ // TODO resolve JAL-2022 so this fudge can be removed
+ int mappedSequenceCount = protein.getHeight() - unmappedProtein.size();
+ addUnmappedPeptideStarts(alignedCodons, mappedSequenceCount);
+
+ return alignedCodons;
+ }
+
+ /**
+ * Scans for any protein mapped from position 2 (meaning unmapped start
+ * position e.g. an incomplete codon), and synthesizes a 'codon' for it at the
+ * preceding position in the alignment
+ *
+ * @param alignedCodons
+ * the codon-to-peptide map
+ * @param mappedSequenceCount
+ * the number of distinct sequences in the map
+ */
+ protected static void addUnmappedPeptideStarts(
+ Map<AlignedCodon, Map<SequenceI, AlignedCodon>> alignedCodons,
+ int mappedSequenceCount)
+ {
+ // TODO delete this ugly hack once JAL-2022 is resolved
+ // i.e. we can model startPhase > 0 (incomplete start codon)
+
+ List<SequenceI> sequencesChecked = new ArrayList<>();
+ AlignedCodon lastCodon = null;
+ Map<SequenceI, AlignedCodon> toAdd = new HashMap<>();
+
+ for (Entry<AlignedCodon, Map<SequenceI, AlignedCodon>> entry : alignedCodons
+ .entrySet())
+ {
+ for (Entry<SequenceI, AlignedCodon> sequenceCodon : entry.getValue()
+ .entrySet())
+ {
+ SequenceI seq = sequenceCodon.getKey();
+ if (sequencesChecked.contains(seq))
+ {
+ continue;
+ }
+ sequencesChecked.add(seq);
+ AlignedCodon codon = sequenceCodon.getValue();
+ if (codon.peptideCol > 1)
+ {
+ System.err.println(
+ "Problem mapping protein with >1 unmapped start positions: "
+ + seq.getName());
+ }
+ else if (codon.peptideCol == 1)
+ {
+ /*
+ * first position (peptideCol == 0) was unmapped - add it
+ */
+ if (lastCodon != null)
+ {
+ AlignedCodon firstPeptide = new AlignedCodon(lastCodon.pos1,
+ lastCodon.pos2, lastCodon.pos3,
+ String.valueOf(seq.getCharAt(0)), 0);
+ toAdd.put(seq, firstPeptide);
+ }
+ else
+ {
+ /*
+ * unmapped residue at start of alignment (no prior column) -
+ * 'insert' at nominal codon [0, 0, 0]
+ */
+ AlignedCodon firstPeptide = new AlignedCodon(0, 0, 0,
+ String.valueOf(seq.getCharAt(0)), 0);
+ toAdd.put(seq, firstPeptide);
+ }
+ }
+ if (sequencesChecked.size() == mappedSequenceCount)
+ {
+ // no need to check past first mapped position in all sequences
+ break;
+ }
+ }
+ lastCodon = entry.getKey();
+ }
+
+ /*
+ * add any new codons safely after iterating over the map
+ */
+ for (Entry<SequenceI, AlignedCodon> startCodon : toAdd.entrySet())
+ {
+ addCodonToMap(alignedCodons, startCodon.getValue(),
+ startCodon.getKey());
+ }
}
/**
* @return
*/
protected static int alignProteinAs(AlignmentI protein,
- Map<AlignedCodon, Map<SequenceI, String>> alignedCodons,
+ Map<AlignedCodon, Map<SequenceI, AlignedCodon>> alignedCodons,
List<SequenceI> unmappedProtein)
{
/*
- * Prefill aligned sequences with gaps before inserting aligned protein
- * residues.
+ * prefill peptide sequences with gaps
*/
int alignedWidth = alignedCodons.size();
char[] gaps = new char[alignedWidth];
Arrays.fill(gaps, protein.getGapCharacter());
- String allGaps = String.valueOf(gaps);
+ Map<SequenceI, char[]> peptides = new HashMap<>();
for (SequenceI seq : protein.getSequences())
{
if (!unmappedProtein.contains(seq))
{
- seq.setSequence(allGaps);
+ peptides.put(seq, Arrays.copyOf(gaps, gaps.length));
}
}
+ /*
+ * Traverse the codons left to right (as defined by CodonComparator)
+ * and insert peptides in each column where the sequence is mapped.
+ * This gives a peptide 'alignment' where residues are aligned if their
+ * corresponding codons occupy the same columns in the cdna alignment.
+ */
int column = 0;
for (AlignedCodon codon : alignedCodons.keySet())
{
- final Map<SequenceI, String> columnResidues = alignedCodons
+ final Map<SequenceI, AlignedCodon> columnResidues = alignedCodons
.get(codon);
- for (Entry<SequenceI, String> entry : columnResidues.entrySet())
+ for (Entry<SequenceI, AlignedCodon> entry : columnResidues.entrySet())
{
- // place translated codon at its column position in sequence
- entry.getKey().getSequence()[column] = entry.getValue().charAt(0);
+ char residue = entry.getValue().product.charAt(0);
+ peptides.get(entry.getKey())[column] = residue;
}
column++;
}
+
+ /*
+ * and finally set the constructed sequences
+ */
+ for (Entry<SequenceI, char[]> entry : peptides.entrySet())
+ {
+ entry.getKey().setSequence(new String(entry.getValue()));
+ }
+
return 0;
}
*/
static void addCodonPositions(SequenceI dna, SequenceI protein,
char gapChar, Mapping seqMap,
- Map<AlignedCodon, Map<SequenceI, String>> alignedCodons)
+ Map<AlignedCodon, Map<SequenceI, AlignedCodon>> alignedCodons)
{
Iterator<AlignedCodon> codons = seqMap.getCodonIterator(dna, gapChar);
+
+ /*
+ * add codon positions, and their peptide translations, to the alignment
+ * map, while remembering the first codon mapped
+ */
while (codons.hasNext())
{
try
{
AlignedCodon codon = codons.next();
- Map<SequenceI, String> seqProduct = alignedCodons.get(codon);
- if (seqProduct == null)
- {
- seqProduct = new HashMap<SequenceI, String>();
- alignedCodons.put(codon, seqProduct);
- }
- seqProduct.put(protein, codon.product);
+ addCodonToMap(alignedCodons, codon, protein);
} catch (IncompleteCodonException e)
{
// possible incomplete trailing codon - ignore
+ } catch (NoSuchElementException e)
+ {
+ // possibly peptide lacking STOP
}
}
}
/**
+ * Helper method to add a codon-to-peptide entry to the aligned codons map
+ *
+ * @param alignedCodons
+ * @param codon
+ * @param protein
+ */
+ protected static void addCodonToMap(
+ Map<AlignedCodon, Map<SequenceI, AlignedCodon>> alignedCodons,
+ AlignedCodon codon, SequenceI protein)
+ {
+ Map<SequenceI, AlignedCodon> seqProduct = alignedCodons.get(codon);
+ if (seqProduct == null)
+ {
+ seqProduct = new HashMap<>();
+ alignedCodons.put(codon, seqProduct);
+ }
+ seqProduct.put(protein, codon);
+ }
+
+ /**
* Returns true if a cDNA/Protein mapping either exists, or could be made,
* between at least one pair of sequences in the two alignments. Currently,
* the logic is:
* <ul>
* <li>One alignment must be nucleotide, and the other protein</li>
* <li>At least one pair of sequences must be already mapped, or mappable</li>
- * <li>Mappable means the nucleotide translation matches the protein sequence</li>
+ * <li>Mappable means the nucleotide translation matches the protein
+ * sequence</li>
* <li>The translation may ignore start and stop codons if present in the
* nucleotide</li>
* </ul>
return false;
}
- SequenceI dnaDs = dnaSeq.getDatasetSequence() == null ? dnaSeq : dnaSeq
- .getDatasetSequence();
- SequenceI proteinDs = proteinSeq.getDatasetSequence() == null ? proteinSeq
+ SequenceI dnaDs = dnaSeq.getDatasetSequence() == null ? dnaSeq
+ : dnaSeq.getDatasetSequence();
+ SequenceI proteinDs = proteinSeq.getDatasetSequence() == null
+ ? proteinSeq
: proteinSeq.getDatasetSequence();
for (AlignedCodonFrame mapping : mappings)
* Just try to make a mapping (it is not yet stored), test whether
* successful.
*/
- return mapProteinSequenceToCdna(proteinDs, dnaDs) != null;
+ return mapCdnaToProtein(proteinDs, dnaDs) != null;
}
/**
* the alignment to check for presence of annotations
*/
public static void findAddableReferenceAnnotations(
- List<SequenceI> sequenceScope,
- Map<String, String> labelForCalcId,
+ List<SequenceI> sequenceScope, Map<String, String> labelForCalcId,
final Map<SequenceI, List<AlignmentAnnotation>> candidates,
AlignmentI al)
{
{
continue;
}
- final List<AlignmentAnnotation> result = new ArrayList<AlignmentAnnotation>();
+ final List<AlignmentAnnotation> result = new ArrayList<>();
for (AlignmentAnnotation dsann : datasetAnnotations)
{
/*
/**
* Set visibility of alignment annotations of specified types (labels), for
- * specified sequences. This supports controls like
- * "Show all secondary structure", "Hide all Temp factor", etc.
+ * specified sequences. This supports controls like "Show all secondary
+ * structure", "Hide all Temp factor", etc.
*
* @al the alignment to scan for annotations
* @param types
Collection<String> types, List<SequenceI> forSequences,
boolean anyType, boolean doShow)
{
- for (AlignmentAnnotation aa : al.getAlignmentAnnotation())
+ AlignmentAnnotation[] anns = al.getAlignmentAnnotation();
+ if (anns != null)
{
- if (anyType || types.contains(aa.label))
+ for (AlignmentAnnotation aa : anns)
{
- if ((aa.sequenceRef != null)
- && (forSequences == null || forSequences
- .contains(aa.sequenceRef)))
+ if (anyType || types.contains(aa.label))
{
- aa.visible = doShow;
+ if ((aa.sequenceRef != null) && (forSequences == null
+ || forSequences.contains(aa.sequenceRef)))
+ {
+ aa.visible = doShow;
+ }
}
}
}
/**
* Constructs an alignment consisting of the mapped (CDS) regions in the given
- * nucleotide sequences, and updates mappings to match. The new sequences are
- * aligned as per the original sequences (with gapped columns omitted).
+ * nucleotide sequences, and updates mappings to match. The CDS sequences are
+ * added to the original alignment's dataset, which is shared by the new
+ * alignment. Mappings from nucleotide to CDS, and from CDS to protein, are
+ * added to the alignment dataset.
*
* @param dna
- * aligned dna sequences
- * @param mappings
- * from dna to protein; these are replaced with new mappings
- * @param gapChar
+ * aligned nucleotide (dna or cds) sequences
+ * @param dataset
+ * the alignment dataset the sequences belong to
+ * @param products
+ * (optional) to restrict results to CDS that map to specified
+ * protein products
* @return an alignment whose sequences are the cds-only parts of the dna
* sequences (or null if no mappings are found)
*/
public static AlignmentI makeCdsAlignment(SequenceI[] dna,
- List<AlignedCodonFrame> mappings, char gapChar)
+ AlignmentI dataset, SequenceI[] products)
{
- List<int[]> cdsColumns = findCdsColumns(dna);
-
- /*
- * create CDS sequences and new mappings
- * (from cdna to cds, and cds to peptide)
- */
- List<AlignedCodonFrame> newMappings = new ArrayList<AlignedCodonFrame>();
- List<SequenceI> cdsSequences = new ArrayList<SequenceI>();
-
- for (SequenceI dnaSeq : dna)
+ if (dataset == null || dataset.getDataset() != null)
{
- final SequenceI ds = dnaSeq.getDatasetSequence();
- List<AlignedCodonFrame> seqMappings = MappingUtils
- .findMappingsForSequence(ds, mappings);
- for (AlignedCodonFrame acf : seqMappings)
+ throw new IllegalArgumentException(
+ "IMPLEMENTATION ERROR: dataset.getDataset() must be null!");
+ }
+ List<SequenceI> foundSeqs = new ArrayList<>();
+ List<SequenceI> cdsSeqs = new ArrayList<>();
+ List<AlignedCodonFrame> mappings = dataset.getCodonFrames();
+ HashSet<SequenceI> productSeqs = null;
+ if (products != null)
+ {
+ productSeqs = new HashSet<>();
+ for (SequenceI seq : products)
{
- AlignedCodonFrame newMapping = new AlignedCodonFrame();
- final List<SequenceI> mappedCds = makeCdsSequences(dnaSeq, acf,
- cdsColumns, newMapping, gapChar);
- if (!mappedCds.isEmpty())
- {
- cdsSequences.addAll(mappedCds);
- newMappings.add(newMapping);
- }
+ productSeqs.add(seq.getDatasetSequence() == null ? seq : seq
+ .getDatasetSequence());
}
}
- AlignmentI al = new Alignment(
- cdsSequences.toArray(new SequenceI[cdsSequences.size()]));
- al.setGapCharacter(gapChar);
- al.setDataset(null);
/*
- * Replace the old mappings with the new ones
+ * Construct CDS sequences from mappings on the alignment dataset.
+ * The logic is:
+ * - find the protein product(s) mapped to from each dna sequence
+ * - if the mapping covers the whole dna sequence (give or take start/stop
+ * codon), take the dna as the CDS sequence
+ * - else search dataset mappings for a suitable dna sequence, i.e. one
+ * whose whole sequence is mapped to the protein
+ * - if no sequence found, construct one from the dna sequence and mapping
+ * (and add it to dataset so it is found if this is repeated)
*/
- mappings.clear();
- mappings.addAll(newMappings);
+ for (SequenceI dnaSeq : dna)
+ {
+ SequenceI dnaDss = dnaSeq.getDatasetSequence() == null ? dnaSeq
+ : dnaSeq.getDatasetSequence();
- return al;
- }
+ List<AlignedCodonFrame> seqMappings = MappingUtils
+ .findMappingsForSequence(dnaSeq, mappings);
+ for (AlignedCodonFrame mapping : seqMappings)
+ {
+ List<Mapping> mappingsFromSequence = mapping
+ .getMappingsFromSequence(dnaSeq);
- /**
- * Returns a consolidated list of column ranges where at least one sequence
- * has a CDS feature. This assumes CDS features are on genomic sequence i.e.
- * are for contiguous CDS ranges (no gaps).
- *
- * @param seqs
- * @return
- */
- public static List<int[]> findCdsColumns(SequenceI[] seqs)
+ for (Mapping aMapping : mappingsFromSequence)
+ {
+ MapList mapList = aMapping.getMap();
+ if (mapList.getFromRatio() == 1)
+ {
+ /*
+ * not a dna-to-protein mapping (likely dna-to-cds)
+ */
+ continue;
+ }
+
+ /*
+ * skip if mapping is not to one of the target set of proteins
+ */
+ SequenceI proteinProduct = aMapping.getTo();
+ if (productSeqs != null && !productSeqs.contains(proteinProduct))
+ {
+ continue;
+ }
+
+ /*
+ * try to locate the CDS from the dataset mappings;
+ * guard against duplicate results (for the case that protein has
+ * dbrefs to both dna and cds sequences)
+ */
+ SequenceI cdsSeq = findCdsForProtein(mappings, dnaSeq,
+ seqMappings, aMapping);
+ if (cdsSeq != null)
+ {
+ if (!foundSeqs.contains(cdsSeq))
+ {
+ foundSeqs.add(cdsSeq);
+ SequenceI derivedSequence = cdsSeq.deriveSequence();
+ cdsSeqs.add(derivedSequence);
+ if (!dataset.getSequences().contains(cdsSeq))
+ {
+ dataset.addSequence(cdsSeq);
+ }
+ }
+ continue;
+ }
+
+ /*
+ * didn't find mapped CDS sequence - construct it and add
+ * its dataset sequence to the dataset
+ */
+ cdsSeq = makeCdsSequence(dnaSeq.getDatasetSequence(), aMapping,
+ dataset).deriveSequence();
+ // cdsSeq has a name constructed as CDS|<dbref>
+ // <dbref> will be either the accession for the coding sequence,
+ // marked in the /via/ dbref to the protein product accession
+ // or it will be the original nucleotide accession.
+ SequenceI cdsSeqDss = cdsSeq.getDatasetSequence();
+
+ cdsSeqs.add(cdsSeq);
+
+ /*
+ * build the mapping from CDS to protein
+ */
+ List<int[]> cdsRange = Collections
+ .singletonList(new int[]
+ { cdsSeq.getStart(),
+ cdsSeq.getLength() + cdsSeq.getStart() - 1 });
+ MapList cdsToProteinMap = new MapList(cdsRange,
+ mapList.getToRanges(), mapList.getFromRatio(),
+ mapList.getToRatio());
+
+ if (!dataset.getSequences().contains(cdsSeqDss))
+ {
+ /*
+ * if this sequence is a newly created one, add it to the dataset
+ * and made a CDS to protein mapping (if sequence already exists,
+ * CDS-to-protein mapping _is_ the transcript-to-protein mapping)
+ */
+ dataset.addSequence(cdsSeqDss);
+ AlignedCodonFrame cdsToProteinMapping = new AlignedCodonFrame();
+ cdsToProteinMapping.addMap(cdsSeqDss, proteinProduct,
+ cdsToProteinMap);
+
+ /*
+ * guard against duplicating the mapping if repeating this action
+ */
+ if (!mappings.contains(cdsToProteinMapping))
+ {
+ mappings.add(cdsToProteinMapping);
+ }
+ }
+
+ propagateDBRefsToCDS(cdsSeqDss, dnaSeq.getDatasetSequence(),
+ proteinProduct, aMapping);
+ /*
+ * add another mapping from original 'from' range to CDS
+ */
+ AlignedCodonFrame dnaToCdsMapping = new AlignedCodonFrame();
+ final MapList dnaToCdsMap = new MapList(mapList.getFromRanges(),
+ cdsRange, 1, 1);
+ dnaToCdsMapping.addMap(dnaSeq.getDatasetSequence(), cdsSeqDss,
+ dnaToCdsMap);
+ if (!mappings.contains(dnaToCdsMapping))
+ {
+ mappings.add(dnaToCdsMapping);
+ }
+
+ /*
+ * transfer dna chromosomal loci (if known) to the CDS
+ * sequence (via the mapping)
+ */
+ final MapList cdsToDnaMap = dnaToCdsMap.getInverse();
+ transferGeneLoci(dnaSeq, cdsToDnaMap, cdsSeq);
+
+ /*
+ * add DBRef with mapping from protein to CDS
+ * (this enables Get Cross-References from protein alignment)
+ * This is tricky because we can't have two DBRefs with the
+ * same source and accession, so need a different accession for
+ * the CDS from the dna sequence
+ */
+
+ // specific use case:
+ // Genomic contig ENSCHR:1, contains coding regions for ENSG01,
+ // ENSG02, ENSG03, with transcripts and products similarly named.
+ // cannot add distinct dbrefs mapping location on ENSCHR:1 to ENSG01
+
+ // JBPNote: ?? can't actually create an example that demonstrates we
+ // need to
+ // synthesize an xref.
+
+ for (DBRefEntry primRef : dnaDss.getPrimaryDBRefs())
+ {
+ /*
+ * create a cross-reference from CDS to the source sequence's
+ * primary reference and vice versa
+ */
+ String source = primRef.getSource();
+ String version = primRef.getVersion();
+ DBRefEntry cdsCrossRef = new DBRefEntry(source, source + ":"
+ + version, primRef.getAccessionId());
+ cdsCrossRef.setMap(new Mapping(dnaDss, new MapList(cdsToDnaMap)));
+ cdsSeqDss.addDBRef(cdsCrossRef);
+
+ dnaSeq.addDBRef(new DBRefEntry(source, version, cdsSeq
+ .getName(), new Mapping(cdsSeqDss, dnaToCdsMap)));
+
+ // problem here is that the cross-reference is synthesized -
+ // cdsSeq.getName() may be like 'CDS|dnaaccession' or
+ // 'CDS|emblcdsacc'
+ // assuming cds version same as dna ?!?
+
+ DBRefEntry proteinToCdsRef = new DBRefEntry(source, version,
+ cdsSeq.getName());
+ //
+ proteinToCdsRef.setMap(new Mapping(cdsSeqDss, cdsToProteinMap
+ .getInverse()));
+ proteinProduct.addDBRef(proteinToCdsRef);
+ }
+
+ /*
+ * transfer any features on dna that overlap the CDS
+ */
+ transferFeatures(dnaSeq, cdsSeq, dnaToCdsMap, null,
+ SequenceOntologyI.CDS);
+ }
+ }
+ }
+
+ AlignmentI cds = new Alignment(cdsSeqs.toArray(new SequenceI[cdsSeqs
+ .size()]));
+ cds.setDataset(dataset);
+
+ return cds;
+ }
+
+ /**
+ * Tries to transfer gene loci (dbref to chromosome positions) from fromSeq to
+ * toSeq, mediated by the given mapping between the sequences
+ *
+ * @param fromSeq
+ * @param targetToFrom
+ * Map
+ * @param targetSeq
+ */
+ protected static void transferGeneLoci(SequenceI fromSeq,
+ MapList targetToFrom, SequenceI targetSeq)
{
- // TODO use refactored code from AlignViewController
- // markColumnsContainingFeatures, not reinvent the wheel!
+ if (targetSeq.getGeneLoci() != null)
+ {
+ // already have - don't override
+ return;
+ }
+ GeneLociI fromLoci = fromSeq.getGeneLoci();
+ if (fromLoci == null)
+ {
+ return;
+ }
+
+ MapList newMap = targetToFrom.traverse(fromLoci.getMapping());
- List<int[]> result = new ArrayList<int[]>();
- for (SequenceI seq : seqs)
+ if (newMap != null)
{
- result.addAll(findCdsColumns(seq));
+ targetSeq.setGeneLoci(fromLoci.getSpeciesId(),
+ fromLoci.getAssemblyId(), fromLoci.getChromosomeId(), newMap);
}
+ }
+
+ /**
+ * A helper method that finds a CDS sequence in the alignment dataset that is
+ * mapped to the given protein sequence, and either is, or has a mapping from,
+ * the given dna sequence.
+ *
+ * @param mappings
+ * set of all mappings on the dataset
+ * @param dnaSeq
+ * a dna (or cds) sequence we are searching from
+ * @param seqMappings
+ * the set of mappings involving dnaSeq
+ * @param aMapping
+ * a transcript-to-peptide mapping
+ * @return
+ */
+ static SequenceI findCdsForProtein(List<AlignedCodonFrame> mappings,
+ SequenceI dnaSeq, List<AlignedCodonFrame> seqMappings,
+ Mapping aMapping)
+ {
+ /*
+ * TODO a better dna-cds-protein mapping data representation to allow easy
+ * navigation; until then this clunky looping around lists of mappings
+ */
+ SequenceI seqDss = dnaSeq.getDatasetSequence() == null ? dnaSeq
+ : dnaSeq.getDatasetSequence();
+ SequenceI proteinProduct = aMapping.getTo();
/*
- * sort and compact the list into ascending, non-overlapping ranges
+ * is this mapping from the whole dna sequence (i.e. CDS)?
+ * allowing for possible stop codon on dna but not peptide
*/
- Collections.sort(result, new Comparator<int[]>()
+ int mappedFromLength = MappingUtils
+ .getLength(aMapping.getMap().getFromRanges());
+ int dnaLength = seqDss.getLength();
+ if (mappedFromLength == dnaLength
+ || mappedFromLength == dnaLength - CODON_LENGTH)
{
- @Override
- public int compare(int[] o1, int[] o2)
+ /*
+ * if sequence has CDS features, this is a transcript with no UTR
+ * - do not take this as the CDS sequence! (JAL-2789)
+ */
+ if (seqDss.getFeatures().getFeaturesByOntology(SequenceOntologyI.CDS)
+ .isEmpty())
{
- return Integer.compare(o1[0], o2[0]);
+ return seqDss;
}
- });
- result = MapList.coalesceRanges(result);
-
- return result;
- }
+ }
- public static List<int[]> findCdsColumns(SequenceI seq)
- {
- List<int[]> result = new ArrayList<int[]>();
- SequenceOntologyI so = SequenceOntologyFactory.getInstance();
- SequenceFeature[] sfs = seq.getSequenceFeatures();
- if (sfs != null)
+ /*
+ * looks like we found the dna-to-protein mapping; search for the
+ * corresponding cds-to-protein mapping
+ */
+ List<AlignedCodonFrame> mappingsToPeptide = MappingUtils
+ .findMappingsForSequence(proteinProduct, mappings);
+ for (AlignedCodonFrame acf : mappingsToPeptide)
{
- for (SequenceFeature sf : sfs)
+ for (SequenceToSequenceMapping map : acf.getMappings())
{
- if (so.isA(sf.getType(), SequenceOntologyI.CDS))
+ Mapping mapping = map.getMapping();
+ if (mapping != aMapping
+ && mapping.getMap().getFromRatio() == CODON_LENGTH
+ && proteinProduct == mapping.getTo()
+ && seqDss != map.getFromSeq())
{
- int colStart = seq.findIndex(sf.getBegin());
- int colEnd = seq.findIndex(sf.getEnd());
- result.add(new int[] { colStart, colEnd });
+ mappedFromLength = MappingUtils
+ .getLength(mapping.getMap().getFromRanges());
+ if (mappedFromLength == map.getFromSeq().getLength())
+ {
+ /*
+ * found a 3:1 mapping to the protein product which covers
+ * the whole dna sequence i.e. is from CDS; finally check the CDS
+ * is mapped from the given dna start sequence
+ */
+ SequenceI cdsSeq = map.getFromSeq();
+ // todo this test is weak if seqMappings contains multiple mappings;
+ // we get away with it if transcript:cds relationship is 1:1
+ List<AlignedCodonFrame> dnaToCdsMaps = MappingUtils
+ .findMappingsForSequence(cdsSeq, seqMappings);
+ if (!dnaToCdsMaps.isEmpty())
+ {
+ return cdsSeq;
+ }
+ }
}
}
}
- return result;
+ return null;
}
/**
- * Answers true if all sequences have a gap at (or do not extend to) the
- * specified column position (base 1)
+ * Helper method that makes a CDS sequence as defined by the mappings from the
+ * given sequence i.e. extracts the 'mapped from' ranges (which may be on
+ * forward or reverse strand).
*
- * @param seqs
- * @param col
- * @return
+ * @param seq
+ * @param mapping
+ * @param dataset
+ * - existing dataset. We check for sequences that look like the CDS
+ * we are about to construct, if one exists already, then we will
+ * just return that one.
+ * @return CDS sequence (as a dataset sequence)
*/
- public static boolean isGappedColumn(List<SequenceI> seqs, int col)
+ static SequenceI makeCdsSequence(SequenceI seq, Mapping mapping,
+ AlignmentI dataset)
{
- if (seqs != null)
+ /*
+ * construct CDS sequence name as "CDS|" with 'from id' held in the mapping
+ * if set (e.g. EMBL protein_id), else sequence name appended
+ */
+ String mapFromId = mapping.getMappedFromId();
+ final String seqId = "CDS|"
+ + (mapFromId != null ? mapFromId : seq.getName());
+
+ SequenceI newSeq = null;
+
+ /*
+ * construct CDS sequence by splicing mapped from ranges
+ */
+ char[] seqChars = seq.getSequence();
+ List<int[]> fromRanges = mapping.getMap().getFromRanges();
+ int cdsWidth = MappingUtils.getLength(fromRanges);
+ char[] newSeqChars = new char[cdsWidth];
+
+ int newPos = 0;
+ for (int[] range : fromRanges)
{
- for (SequenceI seq : seqs)
+ if (range[0] <= range[1])
+ {
+ // forward strand mapping - just copy the range
+ int length = range[1] - range[0] + 1;
+ System.arraycopy(seqChars, range[0] - 1, newSeqChars, newPos,
+ length);
+ newPos += length;
+ }
+ else
{
- if (!Comparison.isGap(seq.getCharAt(col - 1)))
+ // reverse strand mapping - copy and complement one by one
+ for (int i = range[0]; i >= range[1]; i--)
{
- return false;
+ newSeqChars[newPos++] = Dna.getComplement(seqChars[i - 1]);
}
}
+
+ newSeq = new Sequence(seqId, newSeqChars, 1, newPos);
}
- return true;
- }
- /**
- * Returns the column ranges (base 1) of each aligned sequence that are
- * involved in any mapping. This is a helper method for aligning protein
- * products of aligned transcripts.
- *
- * @param mappedSequences
- * (possibly gapped) dna sequences
- * @param mappings
- * @return
- */
- protected static List<List<int[]>> getMappedColumns(
- List<SequenceI> mappedSequences,
- List<AlignedCodonFrame> mappings)
- {
- List<List<int[]>> result = new ArrayList<List<int[]>>();
- for (SequenceI seq : mappedSequences)
+ if (dataset != null)
{
- List<int[]> columns = new ArrayList<int[]>();
- List<AlignedCodonFrame> seqMappings = MappingUtils
- .findMappingsForSequence(seq, mappings);
- for (AlignedCodonFrame mapping : seqMappings)
+ SequenceI[] matches = dataset.findSequenceMatch(newSeq.getName());
+ if (matches != null)
{
- List<Mapping> maps = mapping.getMappingsForSequence(seq);
- for (Mapping map : maps)
+ boolean matched = false;
+ for (SequenceI mtch : matches)
{
- /*
- * Get the codon regions as { [2, 5], [7, 12], [14, 14] etc }
- * Find and add the overall aligned column range for each
- */
- for (int[] cdsRange : map.getMap().getFromRanges())
+ if (mtch.getStart() != newSeq.getStart())
+ {
+ continue;
+ }
+ if (mtch.getEnd() != newSeq.getEnd())
+ {
+ continue;
+ }
+ if (!Arrays.equals(mtch.getSequence(), newSeq.getSequence()))
{
- int startPos = cdsRange[0];
- int endPos = cdsRange[1];
- int startCol = seq.findIndex(startPos);
- int endCol = seq.findIndex(endPos);
- columns.add(new int[] { startCol, endCol });
+ continue;
+ }
+ if (!matched)
+ {
+ matched = true;
+ newSeq = mtch;
+ }
+ else
+ {
+ Cache.log.error(
+ "JAL-2154 regression: warning - found (and ignored) a duplicate CDS sequence:" + mtch.toString());
}
}
}
- result.add(columns);
}
- return result;
+ // newSeq.setDescription(mapFromId);
+
+ return newSeq;
}
/**
- * Helper method to make cds-only sequences and populate their mappings to
- * protein products
- * <p>
- * For example, if ggCCaTTcGAg has mappings [3, 4, 6, 7, 9, 10] to protein
- * then generate a sequence CCTTGA with mapping [1, 6] to the same protein
- * residues
- * <p>
- * Typically eukaryotic dna will include cds encoding for a single peptide
- * sequence i.e. return a single result. Bacterial dna may have overlapping
- * cds mappings coding for multiple peptides so return multiple results
- * (example EMBL KF591215).
+ * Adds any DBRefEntrys to cdsSeq from contig that have a Mapping congruent to
+ * the given mapping.
*
- * @param dnaSeq
- * a dna aligned sequence
+ * @param cdsSeq
+ * @param contig
+ * @param proteinProduct
* @param mapping
- * containing one or more mappings of the sequence to protein
- * @param ungappedCdsColumns
- * @param newMappings
- * the new mapping to populate, from the cds-only sequences to their
- * mapped protein sequences
- * @return
+ * @return list of DBRefEntrys added
*/
- protected static List<SequenceI> makeCdsSequences(SequenceI dnaSeq,
- AlignedCodonFrame mapping, List<int[]> ungappedCdsColumns,
- AlignedCodonFrame newMappings, char gapChar)
+ protected static List<DBRefEntry> propagateDBRefsToCDS(SequenceI cdsSeq,
+ SequenceI contig, SequenceI proteinProduct, Mapping mapping)
{
- List<SequenceI> cdsSequences = new ArrayList<SequenceI>();
- List<Mapping> seqMappings = mapping.getMappingsForSequence(dnaSeq);
- for (Mapping seqMapping : seqMappings)
- {
- SequenceI cds = makeCdsSequence(dnaSeq, seqMapping,
- ungappedCdsColumns, gapChar);
- cdsSequences.add(cds);
+ // gather direct refs from contig congruent with mapping
+ List<DBRefEntry> direct = new ArrayList<>();
+ HashSet<String> directSources = new HashSet<>();
- /*
- * add new mappings, from dna to cds, and from cds to peptide
- */
- MapList dnaToCds = addCdsMappings(dnaSeq.getDatasetSequence(), cds,
- seqMapping, newMappings);
+ if (contig.getDBRefs() != null)
+ {
+ for (DBRefEntry dbr : contig.getDBRefs())
+ {
+ if (dbr.hasMap() && dbr.getMap().getMap().isTripletMap())
+ {
+ MapList map = dbr.getMap().getMap();
+ // check if map is the CDS mapping
+ if (mapping.getMap().equals(map))
+ {
+ direct.add(dbr);
+ directSources.add(dbr.getSource());
+ }
+ }
+ }
+ }
+ DBRefEntry[] onSource = DBRefUtils.selectRefs(
+ proteinProduct.getDBRefs(),
+ directSources.toArray(new String[0]));
+ List<DBRefEntry> propagated = new ArrayList<>();
- /*
- * transfer any features on dna that overlap the CDS
- */
- transferFeatures(dnaSeq, cds, dnaToCds, null, SequenceOntologyI.CDS);
+ // and generate appropriate mappings
+ for (DBRefEntry cdsref : direct)
+ {
+ // clone maplist and mapping
+ MapList cdsposmap = new MapList(
+ Arrays.asList(new int[][]
+ { new int[] { cdsSeq.getStart(), cdsSeq.getEnd() } }),
+ cdsref.getMap().getMap().getToRanges(), 3, 1);
+ Mapping cdsmap = new Mapping(cdsref.getMap().getTo(),
+ cdsref.getMap().getMap());
+
+ // create dbref
+ DBRefEntry newref = new DBRefEntry(cdsref.getSource(),
+ cdsref.getVersion(), cdsref.getAccessionId(),
+ new Mapping(cdsmap.getTo(), cdsposmap));
+
+ // and see if we can map to the protein product for this mapping.
+ // onSource is the filtered set of accessions on protein that we are
+ // tranferring, so we assume accession is the same.
+ if (cdsmap.getTo() == null && onSource != null)
+ {
+ List<DBRefEntry> sourceRefs = DBRefUtils.searchRefs(onSource,
+ cdsref.getAccessionId());
+ if (sourceRefs != null)
+ {
+ for (DBRefEntry srcref : sourceRefs)
+ {
+ if (srcref.getSource().equalsIgnoreCase(cdsref.getSource()))
+ {
+ // we have found a complementary dbref on the protein product, so
+ // update mapping's getTo
+ newref.getMap().setTo(proteinProduct);
+ }
+ }
+ }
+ }
+ cdsSeq.addDBRef(newref);
+ propagated.add(newref);
}
- return cdsSequences;
+ return propagated;
}
/**
*
* @param fromSeq
* @param toSeq
+ * @param mapping
+ * the mapping from 'fromSeq' to 'toSeq'
* @param select
* if not null, only features of this type are copied (including
* subtypes in the Sequence Ontology)
- * @param mapping
- * the mapping from 'fromSeq' to 'toSeq'
* @param omitting
*/
- public static int transferFeatures(SequenceI fromSeq, SequenceI toSeq,
+ protected static int transferFeatures(SequenceI fromSeq, SequenceI toSeq,
MapList mapping, String select, String... omitting)
{
SequenceI copyTo = toSeq;
{
copyTo = copyTo.getDatasetSequence();
}
+ if (fromSeq == copyTo || fromSeq.getDatasetSequence() == copyTo)
+ {
+ return 0; // shared dataset sequence
+ }
+
+ /*
+ * get features, optionally restricted by an ontology term
+ */
+ List<SequenceFeature> sfs = select == null ? fromSeq.getFeatures()
+ .getPositionalFeatures() : fromSeq.getFeatures()
+ .getFeaturesByOntology(select);
- SequenceOntologyI so = SequenceOntologyFactory.getInstance();
int count = 0;
- SequenceFeature[] sfs = fromSeq.getSequenceFeatures();
- if (sfs != null)
+ for (SequenceFeature sf : sfs)
{
- for (SequenceFeature sf : sfs)
+ String type = sf.getType();
+ boolean omit = false;
+ for (String toOmit : omitting)
{
- String type = sf.getType();
- if (select != null && !so.isA(type, select))
+ if (type.equals(toOmit))
{
- continue;
+ omit = true;
}
- boolean omit = false;
- for (String toOmit : omitting)
+ }
+ if (omit)
+ {
+ continue;
+ }
+
+ /*
+ * locate the mapped range - null if either start or end is
+ * not mapped (no partial overlaps are calculated)
+ */
+ int start = sf.getBegin();
+ int end = sf.getEnd();
+ int[] mappedTo = mapping.locateInTo(start, end);
+ /*
+ * if whole exon range doesn't map, try interpreting it
+ * as 5' or 3' exon overlapping the CDS range
+ */
+ if (mappedTo == null)
+ {
+ mappedTo = mapping.locateInTo(end, end);
+ if (mappedTo != null)
{
- if (type.equals(toOmit))
- {
- omit = true;
- }
+ /*
+ * end of exon is in CDS range - 5' overlap
+ * to a range from the start of the peptide
+ */
+ mappedTo[0] = 1;
}
- if (omit)
+ }
+ if (mappedTo == null)
+ {
+ mappedTo = mapping.locateInTo(start, start);
+ if (mappedTo != null)
{
- continue;
+ /*
+ * start of exon is in CDS range - 3' overlap
+ * to a range up to the end of the peptide
+ */
+ mappedTo[1] = toSeq.getLength();
}
+ }
+ if (mappedTo != null)
+ {
+ int newBegin = Math.min(mappedTo[0], mappedTo[1]);
+ int newEnd = Math.max(mappedTo[0], mappedTo[1]);
+ SequenceFeature copy = new SequenceFeature(sf, newBegin, newEnd,
+ sf.getFeatureGroup(), sf.getScore());
+ copyTo.addSequenceFeature(copy);
+ count++;
+ }
+ }
+ return count;
+ }
- /*
- * locate the mapped range - null if either start or end is
- * not mapped (no partial overlaps are calculated)
- */
- int start = sf.getBegin();
- int end = sf.getEnd();
- int[] mappedTo = mapping.locateInTo(start, end);
- /*
- * if whole exon range doesn't map, try interpreting it
- * as 5' or 3' exon overlapping the CDS range
- */
- if (mappedTo == null)
+ /**
+ * Returns a mapping from dna to protein by inspecting sequence features of
+ * type "CDS" on the dna. A mapping is constructed if the total CDS feature
+ * length is 3 times the peptide length (optionally after dropping a trailing
+ * stop codon). This method does not check whether the CDS nucleotide sequence
+ * translates to the peptide sequence.
+ *
+ * @param dnaSeq
+ * @param proteinSeq
+ * @return
+ */
+ public static MapList mapCdsToProtein(SequenceI dnaSeq,
+ SequenceI proteinSeq)
+ {
+ List<int[]> ranges = findCdsPositions(dnaSeq);
+ int mappedDnaLength = MappingUtils.getLength(ranges);
+
+ /*
+ * if not a whole number of codons, truncate mapping
+ */
+ int codonRemainder = mappedDnaLength % CODON_LENGTH;
+ if (codonRemainder > 0)
+ {
+ mappedDnaLength -= codonRemainder;
+ MappingUtils.removeEndPositions(codonRemainder, ranges);
+ }
+
+ int proteinLength = proteinSeq.getLength();
+ int proteinStart = proteinSeq.getStart();
+ int proteinEnd = proteinSeq.getEnd();
+
+ /*
+ * incomplete start codon may mean X at start of peptide
+ * we ignore both for mapping purposes
+ */
+ if (proteinSeq.getCharAt(0) == 'X')
+ {
+ // todo JAL-2022 support startPhase > 0
+ proteinStart++;
+ proteinLength--;
+ }
+ List<int[]> proteinRange = new ArrayList<>();
+
+ /*
+ * dna length should map to protein (or protein plus stop codon)
+ */
+ int codesForResidues = mappedDnaLength / CODON_LENGTH;
+ if (codesForResidues == (proteinLength + 1))
+ {
+ // assuming extra codon is for STOP and not in peptide
+ // todo: check trailing codon is indeed a STOP codon
+ codesForResidues--;
+ mappedDnaLength -= CODON_LENGTH;
+ MappingUtils.removeEndPositions(CODON_LENGTH, ranges);
+ }
+
+ if (codesForResidues == proteinLength)
+ {
+ proteinRange.add(new int[] { proteinStart, proteinEnd });
+ return new MapList(ranges, proteinRange, CODON_LENGTH, 1);
+ }
+ return null;
+ }
+
+ /**
+ * Returns a list of CDS ranges found (as sequence positions base 1), i.e. of
+ * [start, end] positions of sequence features of type "CDS" (or a sub-type of
+ * CDS in the Sequence Ontology). The ranges are sorted into ascending start
+ * position order, so this method is only valid for linear CDS in the same
+ * sense as the protein product.
+ *
+ * @param dnaSeq
+ * @return
+ */
+ protected static List<int[]> findCdsPositions(SequenceI dnaSeq)
+ {
+ List<int[]> result = new ArrayList<>();
+
+ List<SequenceFeature> sfs = dnaSeq.getFeatures().getFeaturesByOntology(
+ SequenceOntologyI.CDS);
+ if (sfs.isEmpty())
+ {
+ return result;
+ }
+ SequenceFeatures.sortFeatures(sfs, true);
+
+ for (SequenceFeature sf : sfs)
+ {
+ int phase = 0;
+ try
+ {
+ phase = Integer.parseInt(sf.getPhase());
+ } catch (NumberFormatException e)
+ {
+ // ignore
+ }
+ /*
+ * phase > 0 on first codon means 5' incomplete - skip to the start
+ * of the next codon; example ENST00000496384
+ */
+ int begin = sf.getBegin();
+ int end = sf.getEnd();
+ if (result.isEmpty() && phase > 0)
+ {
+ begin += phase;
+ if (begin > end)
{
- mappedTo = mapping.locateInTo(end, end);
- if (mappedTo != null)
+ // shouldn't happen!
+ System.err
+ .println("Error: start phase extends beyond start CDS in "
+ + dnaSeq.getName());
+ }
+ }
+ result.add(new int[] { begin, end });
+ }
+
+ /*
+ * Finally sort ranges by start position. This avoids a dependency on
+ * keeping features in order on the sequence (if they are in order anyway,
+ * the sort will have almost no work to do). The implicit assumption is CDS
+ * ranges are assembled in order. Other cases should not use this method,
+ * but instead construct an explicit mapping for CDS (e.g. EMBL parsing).
+ */
+ Collections.sort(result, IntRangeComparator.ASCENDING);
+ return result;
+ }
+
+ /**
+ * Makes an alignment with a copy of the given sequences, adding in any
+ * non-redundant sequences which are mapped to by the cross-referenced
+ * sequences.
+ *
+ * @param seqs
+ * @param xrefs
+ * @param dataset
+ * the alignment dataset shared by the new copy
+ * @return
+ */
+ public static AlignmentI makeCopyAlignment(SequenceI[] seqs,
+ SequenceI[] xrefs, AlignmentI dataset)
+ {
+ AlignmentI copy = new Alignment(new Alignment(seqs));
+ copy.setDataset(dataset);
+ boolean isProtein = !copy.isNucleotide();
+ SequenceIdMatcher matcher = new SequenceIdMatcher(seqs);
+ if (xrefs != null)
+ {
+ for (SequenceI xref : xrefs)
+ {
+ DBRefEntry[] dbrefs = xref.getDBRefs();
+ if (dbrefs != null)
+ {
+ for (DBRefEntry dbref : dbrefs)
{
- /*
- * end of exon is in CDS range - 5' overlap
- * to a range from the start of the peptide
- */
- mappedTo[0] = 1;
+ if (dbref.getMap() == null || dbref.getMap().getTo() == null
+ || dbref.getMap().getTo().isProtein() != isProtein)
+ {
+ continue;
+ }
+ SequenceI mappedTo = dbref.getMap().getTo();
+ SequenceI match = matcher.findIdMatch(mappedTo);
+ if (match == null)
+ {
+ matcher.add(mappedTo);
+ copy.addSequence(mappedTo);
+ }
}
}
- if (mappedTo == null)
+ }
+ }
+ return copy;
+ }
+
+ /**
+ * Try to align sequences in 'unaligned' to match the alignment of their
+ * mapped regions in 'aligned'. For example, could use this to align CDS
+ * sequences which are mapped to their parent cDNA sequences.
+ *
+ * This method handles 1:1 mappings (dna-to-dna or protein-to-protein). For
+ * dna-to-protein or protein-to-dna use alternative methods.
+ *
+ * @param unaligned
+ * sequences to be aligned
+ * @param aligned
+ * holds aligned sequences and their mappings
+ * @return
+ */
+ public static int alignAs(AlignmentI unaligned, AlignmentI aligned)
+ {
+ /*
+ * easy case - aligning a copy of aligned sequences
+ */
+ if (alignAsSameSequences(unaligned, aligned))
+ {
+ return unaligned.getHeight();
+ }
+
+ /*
+ * fancy case - aligning via mappings between sequences
+ */
+ List<SequenceI> unmapped = new ArrayList<>();
+ Map<Integer, Map<SequenceI, Character>> columnMap = buildMappedColumnsMap(
+ unaligned, aligned, unmapped);
+ int width = columnMap.size();
+ char gap = unaligned.getGapCharacter();
+ int realignedCount = 0;
+ // TODO: verify this loop scales sensibly for very wide/high alignments
+
+ for (SequenceI seq : unaligned.getSequences())
+ {
+ if (!unmapped.contains(seq))
+ {
+ char[] newSeq = new char[width];
+ Arrays.fill(newSeq, gap); // JBPComment - doubt this is faster than the
+ // Integer iteration below
+ int newCol = 0;
+ int lastCol = 0;
+
+ /*
+ * traverse the map to find columns populated
+ * by our sequence
+ */
+ for (Integer column : columnMap.keySet())
{
- mappedTo = mapping.locateInTo(start, start);
- if (mappedTo != null)
+ Character c = columnMap.get(column).get(seq);
+ if (c != null)
{
/*
- * start of exon is in CDS range - 3' overlap
- * to a range up to the end of the peptide
+ * sequence has a character at this position
+ *
*/
- mappedTo[1] = toSeq.getLength();
+ newSeq[newCol] = c;
+ lastCol = newCol;
}
+ newCol++;
}
- if (mappedTo != null)
+
+ /*
+ * trim trailing gaps
+ */
+ if (lastCol < width)
{
- SequenceFeature copy = new SequenceFeature(sf);
- copy.setBegin(Math.min(mappedTo[0], mappedTo[1]));
- copy.setEnd(Math.max(mappedTo[0], mappedTo[1]));
- copyTo.addSequenceFeature(copy);
- count++;
+ char[] tmp = new char[lastCol + 1];
+ System.arraycopy(newSeq, 0, tmp, 0, lastCol + 1);
+ newSeq = tmp;
}
+ // TODO: optimise SequenceI to avoid char[]->String->char[]
+ seq.setSequence(String.valueOf(newSeq));
+ realignedCount++;
}
}
- return count;
+ return realignedCount;
}
/**
- * Creates and adds mappings
- * <ul>
- * <li>from cds to peptide</li>
- * <li>from dna to cds</li>
- * </ul>
- * and returns the dna-to-cds mapping
+ * If unaligned and aligned sequences share the same dataset sequences, then
+ * simply copies the aligned sequences to the unaligned sequences and returns
+ * true; else returns false
*
- * @param dnaSeq
- * @param cdsSeq
- * @param dnaMapping
- * @param newMappings
+ * @param unaligned
+ * - sequences to be aligned based on aligned
+ * @param aligned
+ * - 'guide' alignment containing sequences derived from same
+ * dataset as unaligned
* @return
*/
- protected static MapList addCdsMappings(SequenceI dnaSeq,
- SequenceI cdsSeq,
- Mapping dnaMapping, AlignedCodonFrame newMappings)
+ static boolean alignAsSameSequences(AlignmentI unaligned,
+ AlignmentI aligned)
{
- cdsSeq.createDatasetSequence();
+ if (aligned.getDataset() == null || unaligned.getDataset() == null)
+ {
+ return false; // should only pass alignments with datasets here
+ }
+
+ // map from dataset sequence to alignment sequence(s)
+ Map<SequenceI, List<SequenceI>> alignedDatasets = new HashMap<>();
+ for (SequenceI seq : aligned.getSequences())
+ {
+ SequenceI ds = seq.getDatasetSequence();
+ if (alignedDatasets.get(ds) == null)
+ {
+ alignedDatasets.put(ds, new ArrayList<SequenceI>());
+ }
+ alignedDatasets.get(ds).add(seq);
+ }
/*
- * CDS to peptide is just a contiguous 3:1 mapping, with
- * the peptide ranges taken unchanged from the dna mapping
+ * first pass - check whether all sequences to be aligned share a
+ * dataset sequence with an aligned sequence; also note the leftmost
+ * ungapped column from which to copy
*/
- List<int[]> cdsRanges = new ArrayList<int[]>();
- SequenceI cdsDataset = cdsSeq.getDatasetSequence();
- cdsRanges.add(new int[] { 1, cdsDataset.getLength() });
- MapList cdsToPeptide = new MapList(cdsRanges, dnaMapping.getMap()
- .getToRanges(), 3, 1);
- newMappings.addMap(cdsDataset, dnaMapping.getTo(),
- cdsToPeptide);
+ int leftmost = Integer.MAX_VALUE;
+ for (SequenceI seq : unaligned.getSequences())
+ {
+ final SequenceI ds = seq.getDatasetSequence();
+ if (!alignedDatasets.containsKey(ds))
+ {
+ return false;
+ }
+ SequenceI alignedSeq = alignedDatasets.get(ds)
+ .get(0);
+ int startCol = alignedSeq.findIndex(seq.getStart()); // 1..
+ leftmost = Math.min(leftmost, startCol);
+ }
/*
- * dna 'from' ranges map 1:1 to the contiguous extracted CDS
+ * second pass - copy aligned sequences;
+ * heuristic rule: pair off sequences in order for the case where
+ * more than one shares the same dataset sequence
*/
- MapList dnaToCds = new MapList(
- dnaMapping.getMap().getFromRanges(), cdsRanges, 1, 1);
- newMappings.addMap(dnaSeq, cdsDataset, dnaToCds);
- return dnaToCds;
+ final char gapCharacter = aligned.getGapCharacter();
+ for (SequenceI seq : unaligned.getSequences())
+ {
+ List<SequenceI> alignedSequences = alignedDatasets
+ .get(seq.getDatasetSequence());
+ if (alignedSequences.isEmpty())
+ {
+ /*
+ * defensive check - shouldn't happen! (JAL-3536)
+ */
+ continue;
+ }
+ SequenceI alignedSeq = alignedSequences.get(0);
+
+ /*
+ * gap fill for leading (5') UTR if any
+ */
+ // TODO this copies intron columns - wrong!
+ int startCol = alignedSeq.findIndex(seq.getStart()); // 1..
+ int endCol = alignedSeq.findIndex(seq.getEnd());
+ char[] seqchars = new char[endCol - leftmost + 1];
+ Arrays.fill(seqchars, gapCharacter);
+ char[] toCopy = alignedSeq.getSequence(startCol - 1, endCol);
+ System.arraycopy(toCopy, 0, seqchars, startCol - leftmost,
+ toCopy.length);
+ seq.setSequence(String.valueOf(seqchars));
+ if (alignedSequences.size() > 0)
+ {
+ // pop off aligned sequences (except the last one)
+ alignedSequences.remove(0);
+ }
+ }
+
+ /*
+ * finally remove gapped columns (e.g. introns)
+ */
+ new RemoveGapColCommand("", unaligned.getSequencesArray(), 0,
+ unaligned.getWidth() - 1, unaligned);
+
+ return true;
}
/**
- * Makes and returns a CDS-only sequence, where the CDS regions are identified
- * as the 'from' ranges of the mapping on the dna.
+ * Returns a map whose key is alignment column number (base 1), and whose
+ * values are a map of sequence characters in that column.
*
- * @param dnaSeq
- * nucleotide sequence
- * @param seqMapping
- * mappings from CDS regions of nucleotide
- * @param ungappedCdsColumns
+ * @param unaligned
+ * @param aligned
+ * @param unmapped
* @return
*/
- protected static SequenceI makeCdsSequence(SequenceI dnaSeq,
- Mapping seqMapping, List<int[]> ungappedCdsColumns, char gapChar)
+ static SortedMap<Integer, Map<SequenceI, Character>> buildMappedColumnsMap(
+ AlignmentI unaligned, AlignmentI aligned,
+ List<SequenceI> unmapped)
{
- int cdsWidth = MappingUtils.getLength(ungappedCdsColumns);
+ /*
+ * Map will hold, for each aligned column position, a map of
+ * {unalignedSequence, characterPerSequence} at that position.
+ * TreeMap keeps the entries in ascending column order.
+ */
+ SortedMap<Integer, Map<SequenceI, Character>> map = new TreeMap<>();
/*
- * populate CDS columns with the aligned
- * column character if that column is mapped (which may be a gap
- * if an intron interrupts a codon), else with a gap
+ * record any sequences that have no mapping so can't be realigned
*/
- List<int[]> fromRanges = seqMapping.getMap().getFromRanges();
- char[] cdsChars = new char[cdsWidth];
- int pos = 0;
- for (int[] columns : ungappedCdsColumns)
+ unmapped.addAll(unaligned.getSequences());
+
+ List<AlignedCodonFrame> mappings = aligned.getCodonFrames();
+
+ for (SequenceI seq : unaligned.getSequences())
{
- for (int i = columns[0]; i <= columns[1]; i++)
+ for (AlignedCodonFrame mapping : mappings)
{
- char dnaChar = dnaSeq.getCharAt(i - 1);
- if (Comparison.isGap(dnaChar))
- {
- cdsChars[pos] = gapChar;
- }
- else
+ SequenceI fromSeq = mapping.findAlignedSequence(seq, aligned);
+ if (fromSeq != null)
{
- int seqPos = dnaSeq.findPosition(i - 1);
- if (MappingUtils.contains(fromRanges, seqPos))
- {
- cdsChars[pos] = dnaChar;
- }
- else
+ Mapping seqMap = mapping.getMappingBetween(fromSeq, seq);
+ if (addMappedPositions(seq, fromSeq, seqMap, map))
{
- cdsChars[pos] = gapChar;
+ unmapped.remove(seq);
}
}
- pos++;
}
}
- SequenceI cdsSequence = new Sequence(dnaSeq.getName(),
- String.valueOf(cdsChars));
-
- transferDbRefs(seqMapping.getTo(), cdsSequence);
-
- return cdsSequence;
+ return map;
}
/**
- * Locate any xrefs to CDS databases on the protein product and attach to the
- * CDS sequence. Also add as a sub-token of the sequence name.
+ * Helper method that adds to a map the mapped column positions of a sequence.
+ * <br>
+ * For example if aaTT-Tg-gAAA is mapped to TTTAAA then the map should record
+ * that columns 3,4,6,10,11,12 map to characters T,T,T,A,A,A of the mapped to
+ * sequence.
*
- * @param from
- * @param to
+ * @param seq
+ * the sequence whose column positions we are recording
+ * @param fromSeq
+ * a sequence that is mapped to the first sequence
+ * @param seqMap
+ * the mapping from 'fromSeq' to 'seq'
+ * @param map
+ * a map to add the column positions (in fromSeq) of the mapped
+ * positions of seq
+ * @return
*/
- protected static void transferDbRefs(SequenceI from, SequenceI to)
+ static boolean addMappedPositions(SequenceI seq, SequenceI fromSeq,
+ Mapping seqMap, Map<Integer, Map<SequenceI, Character>> map)
{
- String cdsAccId = FeatureProperties.getCodingFeature(DBRefSource.EMBL);
- DBRefEntry[] cdsRefs = DBRefUtils.selectRefs(from.getDBRefs(),
- DBRefSource.CODINGDBS);
- if (cdsRefs != null)
+ if (seqMap == null)
{
- for (DBRefEntry cdsRef : cdsRefs)
+ return false;
+ }
+
+ /*
+ * invert mapping if it is from unaligned to aligned sequence
+ */
+ if (seqMap.getTo() == fromSeq.getDatasetSequence())
+ {
+ seqMap = new Mapping(seq.getDatasetSequence(),
+ seqMap.getMap().getInverse());
+ }
+
+ int toStart = seq.getStart();
+
+ /*
+ * traverse [start, end, start, end...] ranges in fromSeq
+ */
+ for (int[] fromRange : seqMap.getMap().getFromRanges())
+ {
+ for (int i = 0; i < fromRange.length - 1; i += 2)
{
- to.addDBRef(new DBRefEntry(cdsRef));
- cdsAccId = cdsRef.getAccessionId();
+ boolean forward = fromRange[i + 1] >= fromRange[i];
+
+ /*
+ * find the range mapped to (sequence positions base 1)
+ */
+ int[] range = seqMap.locateMappedRange(fromRange[i],
+ fromRange[i + 1]);
+ if (range == null)
+ {
+ System.err.println("Error in mapping " + seqMap + " from "
+ + fromSeq.getName());
+ return false;
+ }
+ int fromCol = fromSeq.findIndex(fromRange[i]);
+ int mappedCharPos = range[0];
+
+ /*
+ * walk over the 'from' aligned sequence in forward or reverse
+ * direction; when a non-gap is found, record the column position
+ * of the next character of the mapped-to sequence; stop when all
+ * the characters of the range have been counted
+ */
+ while (mappedCharPos <= range[1] && fromCol <= fromSeq.getLength()
+ && fromCol >= 0)
+ {
+ if (!Comparison.isGap(fromSeq.getCharAt(fromCol - 1)))
+ {
+ /*
+ * mapped from sequence has a character in this column
+ * record the column position for the mapped to character
+ */
+ Map<SequenceI, Character> seqsMap = map.get(fromCol);
+ if (seqsMap == null)
+ {
+ seqsMap = new HashMap<>();
+ map.put(fromCol, seqsMap);
+ }
+ seqsMap.put(seq, seq.getCharAt(mappedCharPos - toStart));
+ mappedCharPos++;
+ }
+ fromCol += (forward ? 1 : -1);
+ }
}
}
- if (!to.getName().contains(cdsAccId))
+ return true;
+ }
+
+ // strictly temporary hack until proper criteria for aligning protein to cds
+ // are in place; this is so Ensembl -> fetch xrefs Uniprot aligns the Uniprot
+ public static boolean looksLikeEnsembl(AlignmentI alignment)
+ {
+ for (SequenceI seq : alignment.getSequences())
{
- to.setName(to.getName() + "|" + cdsAccId);
+ String name = seq.getName();
+ if (!name.startsWith("ENSG") && !name.startsWith("ENST"))
+ {
+ return false;
+ }
}
+ return true;
}
}
git://source.jalview.org / jalview.git / blobdiff