JAL-1432 updated copyright notices
[jalview.git] / src / jalview / appletgui / AlignFrame.java
old mode 100755 (executable)
new mode 100644 (file)
index 1496595..ab99e06
@@ -1,36 +1,94 @@
 /*
- * Jalview - A Sequence Alignment Editor and Viewer (Version 2.6)
- * Copyright (C) 2010 J Procter, AM Waterhouse, G Barton, M Clamp, S Searle
+ * Jalview - A Sequence Alignment Editor and Viewer (Version 2.8.0b1)
+ * Copyright (C) 2014 The Jalview Authors
  * 
  * This file is part of Jalview.
  * 
  * Jalview is free software: you can redistribute it and/or
  * modify it under the terms of the GNU General Public License 
  * as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
- * 
+ *  
  * Jalview is distributed in the hope that it will be useful, but 
  * WITHOUT ANY WARRANTY; without even the implied warranty 
  * of MERCHANTABILITY or FITNESS FOR A PARTICULAR 
  * PURPOSE.  See the GNU General Public License for more details.
  * 
  * You should have received a copy of the GNU General Public License along with Jalview.  If not, see <http://www.gnu.org/licenses/>.
+ * The Jalview Authors are detailed in the 'AUTHORS' file.
  */
 package jalview.appletgui;
 
-import java.io.*;
-import java.net.*;
-import java.util.*;
-
-import java.awt.*;
-import java.awt.event.*;
-
-import jalview.analysis.*;
+import jalview.analysis.AlignmentSorter;
+import jalview.analysis.Conservation;
 import jalview.api.SequenceStructureBinding;
 import jalview.bin.JalviewLite;
-import jalview.commands.*;
-import jalview.datamodel.*;
-import jalview.io.*;
-import jalview.schemes.*;
+import jalview.commands.CommandI;
+import jalview.commands.EditCommand;
+import jalview.commands.OrderCommand;
+import jalview.commands.RemoveGapColCommand;
+import jalview.commands.RemoveGapsCommand;
+import jalview.commands.SlideSequencesCommand;
+import jalview.commands.TrimRegionCommand;
+import jalview.datamodel.Alignment;
+import jalview.datamodel.AlignmentI;
+import jalview.datamodel.AlignmentOrder;
+import jalview.datamodel.ColumnSelection;
+import jalview.datamodel.PDBEntry;
+import jalview.datamodel.Sequence;
+import jalview.datamodel.SequenceGroup;
+import jalview.datamodel.SequenceI;
+import jalview.io.AnnotationFile;
+import jalview.io.AppletFormatAdapter;
+import jalview.io.FeaturesFile;
+import jalview.io.TCoffeeScoreFile;
+import jalview.schemes.Blosum62ColourScheme;
+import jalview.schemes.BuriedColourScheme;
+import jalview.schemes.ClustalxColourScheme;
+import jalview.schemes.ColourSchemeI;
+import jalview.schemes.HelixColourScheme;
+import jalview.schemes.HydrophobicColourScheme;
+import jalview.schemes.NucleotideColourScheme;
+import jalview.schemes.PIDColourScheme;
+import jalview.schemes.PurinePyrimidineColourScheme;
+import jalview.schemes.RNAHelicesColourChooser;
+import jalview.schemes.ResidueProperties;
+import jalview.schemes.StrandColourScheme;
+import jalview.schemes.TCoffeeColourScheme;
+import jalview.schemes.TaylorColourScheme;
+import jalview.schemes.TurnColourScheme;
+import jalview.schemes.ZappoColourScheme;
+import jalview.structure.StructureSelectionManager;
+
+import java.awt.BorderLayout;
+import java.awt.Canvas;
+import java.awt.CheckboxMenuItem;
+import java.awt.Color;
+import java.awt.Font;
+import java.awt.FontMetrics;
+import java.awt.Frame;
+import java.awt.Graphics;
+import java.awt.Label;
+import java.awt.Menu;
+import java.awt.MenuBar;
+import java.awt.MenuItem;
+import java.awt.event.ActionEvent;
+import java.awt.event.ActionListener;
+import java.awt.event.FocusEvent;
+import java.awt.event.FocusListener;
+import java.awt.event.ItemEvent;
+import java.awt.event.ItemListener;
+import java.awt.event.KeyEvent;
+import java.awt.event.KeyListener;
+import java.awt.event.WindowAdapter;
+import java.awt.event.WindowEvent;
+import java.io.IOException;
+import java.net.URL;
+import java.net.URLEncoder;
+import java.util.Enumeration;
+import java.util.Hashtable;
+import java.util.List;
+import java.util.StringTokenizer;
+import java.util.Vector;
 
 public class AlignFrame extends EmbmenuFrame implements ActionListener,
         ItemListener, KeyListener
@@ -48,7 +106,6 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public AlignFrame(AlignmentI al, jalview.bin.JalviewLite applet,
           String title, boolean embedded)
   {
-
     if (applet != null)
     {
       jalviewServletURL = applet.getParameter("APPLICATION_URL");
@@ -69,8 +126,13 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     viewport.updateConsensus(alignPanel);
 
     annotationPanelMenuItem.setState(viewport.showAnnotation);
-    displayNonconservedMenuItem.setState(viewport.getShowunconserved());
+    displayNonconservedMenuItem.setState(viewport.getShowUnconserved());
     followMouseOverFlag.setState(viewport.getFollowHighlight());
+    showGroupConsensus.setState(viewport.isShowGroupConsensus());
+    showGroupConservation.setState(viewport.isShowGroupConservation());
+    showConsensusHistogram.setState(viewport.isShowConsensusHistogram());
+    showSequenceLogo.setState(viewport.isShowSequenceLogo());
+    normSequenceLogo.setState(viewport.isNormaliseSequenceLogo());
 
     seqLimits.setState(viewport.showJVSuffix);
 
@@ -127,7 +189,23 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       }
 
     }
-
+    if (viewport.getAlignment().isNucleotide())
+    {
+      viewport.updateStrucConsensus(alignPanel);
+      if (viewport.getAlignment().hasRNAStructure())
+      {
+        RNAHelixColour.setEnabled(true);
+      }
+      else
+      {
+        RNAHelixColour.setEnabled(false);
+      }
+    }
+    else
+    {
+      RNAHelixColour.setEnabled(false);
+      purinePyrimidineColour.setEnabled(false);
+    }
     // Some JVMS send keyevents to Top frame or lowest panel,
     // Havent worked out why yet. So add to both this frame and seqCanvas for
     // now
@@ -136,8 +214,13 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     alignPanel.idPanel.idCanvas.addKeyListener(this);
     alignPanel.scalePanel.addKeyListener(this);
     alignPanel.annotationPanel.addKeyListener(this);
+    alignPanel.annotationPanelHolder.addKeyListener(this);
+    alignPanel.annotationSpaceFillerHolder.addKeyListener(this);
+    alignPanel.alabels.addKeyListener(this);
     createAlignFrameWindow(embedded, title);
-    alignPanel.validate();
+
+    validate();
+    alignPanel.adjustAnnotationHeight();
     alignPanel.paintAlignment(true);
   }
 
@@ -152,22 +235,46 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   }
 
   /**
-   * DOCUMENT ME!
+   * Load a features file onto the alignment
    * 
-   * @param String
-   *          DOCUMENT ME!
+   * @param file
+   *          file URL, content, or other resolvable path
+   * @param type
+   *          is protocol for accessing data referred to by file
    */
 
-  public void parseFeaturesFile(String file, String type)
+  public boolean parseFeaturesFile(String file, String type)
   {
+    return parseFeaturesFile(file, type, true);
+  }
+
+  /**
+   * Load a features file onto the alignment
+   * 
+   * @param file
+   *          file URL, content, or other resolvable path
+   * @param type
+   *          is protocol for accessing data referred to by file
+   * @param autoenabledisplay
+   *          when true, display features flag will be automatically enabled if
+   *          features are loaded
+   * @return true if data parsed as a features file
+   */
+  public boolean parseFeaturesFile(String file, String type,
+          boolean autoenabledisplay)
+  {
+    // TODO: test if importing a features file onto an alignment which already
+    // has features with links overwrites the original links.
+
     Hashtable featureLinks = new Hashtable();
     boolean featuresFile = false;
     try
     {
       featuresFile = new jalview.io.FeaturesFile(file, type)
-              .parse(viewport.alignment,
-                      alignPanel.seqPanel.seqCanvas.getFeatureRenderer().featureColours,
-                      featureLinks, true);
+              .parse(viewport.getAlignment(), alignPanel.seqPanel.seqCanvas
+                      .getFeatureRenderer().featureColours, featureLinks,
+                      true, viewport.applet.getDefaultParameter(
+                              "relaxedidmatch", false));
     } catch (Exception ex)
     {
       ex.printStackTrace();
@@ -179,17 +286,22 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       {
         alignPanel.seqPanel.seqCanvas.getFeatureRenderer().featureLinks = featureLinks;
       }
-      viewport.showSequenceFeatures = true;
-      sequenceFeatures.setState(true);
+      if (autoenabledisplay)
+      {
+        viewport.showSequenceFeatures = true;
+        sequenceFeatures.setState(true);
+      }
       if (viewport.featureSettings != null)
       {
         viewport.featureSettings.refreshTable();
       }
       alignPanel.paintAlignment(true);
+      statusBar.setText("Successfully added features to alignment.");
     }
-
+    return featuresFile;
   }
 
+  @Override
   public void keyPressed(KeyEvent evt)
   {
     if (viewport.cursorMode
@@ -203,6 +315,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
     case 27: // escape key
       deselectAllSequenceMenuItem_actionPerformed();
+
+      alignPanel.alabels.cancelDrag();
       break;
     case KeyEvent.VK_X:
       if (evt.isControlDown() || evt.isMetaDown())
@@ -462,8 +576,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       // Hide everything by the current selection - this is a hack - we do the
       // invert and then hide
       // first check that there will be visible columns after the invert.
-      if ((viewport.colSel != null && viewport.colSel.getSelected() != null && viewport.colSel
-              .getSelected().size() > 0)
+      if ((viewport.getColumnSelection() != null
+              && viewport.getColumnSelection().getSelected() != null && viewport
+              .getColumnSelection().getSelected().size() > 0)
               || (sg != null && sg.getSize() > 0 && sg.getStartRes() <= sg
                       .getEndRes()))
       {
@@ -486,12 +601,13 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
     if (toggleSeqs)
     {
-      if (sg != null && sg.getSize() != viewport.alignment.getHeight())
+      if (sg != null && sg.getSize() != viewport.getAlignment().getHeight())
       {
         hide = true;
         viewport.hideAllSelectedSeqs();
       }
-      else if (!(toggleCols && viewport.colSel.getSelected().size() > 0))
+      else if (!(toggleCols && viewport.getColumnSelection().getSelected()
+              .size() > 0))
       {
         viewport.showAllHiddenSeqs();
       }
@@ -499,12 +615,12 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
     if (toggleCols)
     {
-      if (viewport.colSel.getSelected().size() > 0)
+      if (viewport.getColumnSelection().getSelected().size() > 0)
       {
         viewport.hideSelectedColumns();
         if (!toggleSeqs)
         {
-          viewport.selectionGroup = sg;
+          viewport.setSelectionGroup(sg);
         }
       }
       else if (!hide)
@@ -514,14 +630,17 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
   }
 
+  @Override
   public void keyReleased(KeyEvent evt)
   {
   }
 
+  @Override
   public void keyTyped(KeyEvent evt)
   {
   }
 
+  @Override
   public void itemStateChanged(ItemEvent evt)
   {
     if (evt.getSource() == displayNonconservedMenuItem)
@@ -588,7 +707,11 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
     else if (evt.getSource() == autoCalculate)
     {
-      viewport.autocalculateConsensus = autoCalculate.getState();
+      viewport.autoCalculateConsensus = autoCalculate.getState();
+    }
+    else if (evt.getSource() == sortByTree)
+    {
+      viewport.sortByTree = sortByTree.getState();
     }
     else if (evt.getSource() == this.centreColumnLabelFlag)
     {
@@ -598,7 +721,30 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       mouseOverFlag_stateChanged();
     }
-
+    else if (evt.getSource() == showGroupConsensus)
+    {
+      showGroupConsensus_actionPerformed();
+    }
+    else if (evt.getSource() == showGroupConservation)
+    {
+      showGroupConservation_actionPerformed();
+    }
+    else if (evt.getSource() == showSequenceLogo)
+    {
+      showSequenceLogo_actionPerformed();
+    }
+    else if (evt.getSource() == normSequenceLogo)
+    {
+      normSequenceLogo_actionPerformed();
+    }
+    else if (evt.getSource() == showConsensusHistogram)
+    {
+      showConsensusHistogram_actionPerformed();
+    }
+    else if (evt.getSource() == applyAutoAnnotationSettings)
+    {
+      applyAutoAnnotationSettings_actionPerformed();
+    }
     alignPanel.paintAlignment(true);
   }
 
@@ -615,6 +761,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     this.alignPanel.annotationPanel.repaint();
   }
 
+  @Override
   public void actionPerformed(ActionEvent evt)
   {
     Object source = evt.getSource();
@@ -788,6 +935,30 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       viewport.showAllHiddenSeqs();
       alignPanel.paintAlignment(true);
     }
+    else if (source == showGroupConsensus)
+    {
+      showGroupConsensus_actionPerformed();
+    }
+    else if (source == showGroupConservation)
+    {
+      showGroupConservation_actionPerformed();
+    }
+    else if (source == showSequenceLogo)
+    {
+      showSequenceLogo_actionPerformed();
+    }
+    else if (source == normSequenceLogo)
+    {
+      normSequenceLogo_actionPerformed();
+    }
+    else if (source == showConsensusHistogram)
+    {
+      showConsensusHistogram_actionPerformed();
+    }
+    else if (source == applyAutoAnnotationSettings)
+    {
+      applyAutoAnnotationSettings_actionPerformed();
+    }
     else if (source == featureSettings)
     {
       new FeatureSettings(alignPanel);
@@ -795,7 +966,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     else if (source == alProperties)
     {
       StringBuffer contents = new jalview.io.AlignmentProperties(
-              viewport.alignment).formatAsString();
+              viewport.getAlignment()).formatAsString();
       CutAndPasteTransfer cap = new CutAndPasteTransfer(false, this);
       cap.setText(contents.toString());
       Frame frame = new Frame();
@@ -814,9 +985,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     else if (source == clustalColour)
     {
       abovePIDThreshold.setState(false);
-      changeColour(new ClustalxColourScheme(
-              viewport.alignment.getSequences(),
-              viewport.alignment.getWidth()));
+      changeColour(new ClustalxColourScheme(viewport.getAlignment(), null));
     }
     else if (source == zappoColour)
     {
@@ -850,6 +1019,14 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       changeColour(new NucleotideColourScheme());
     }
+    else if (source == purinePyrimidineColour)
+    {
+      changeColour(new PurinePyrimidineColourScheme());
+    }
+    else if (source == RNAHelixColour)
+    {
+      new RNAHelicesColourChooser(viewport, alignPanel);
+    }
     else if (source == modifyPID)
     {
       modifyPID_actionPerformed();
@@ -870,6 +1047,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       changeColour(new Blosum62ColourScheme());
     }
+    else if (source == tcoffeeColour)
+    {
+      changeColour(new TCoffeeColourScheme(alignPanel.getAlignment()));
+    }
     else if (source == annotationColour)
     {
       new AnnotationColourChooser(viewport, alignPanel);
@@ -952,7 +1133,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public void loadAnnotations()
   {
     CutAndPasteTransfer cap = new CutAndPasteTransfer(true, this);
-    cap.setText("Paste your features / annotations file here.");
+    cap.setText("Paste your features / annotations / T-coffee score file here.");
     cap.setAnnotationImport();
     Frame frame = new Frame();
     frame.add(cap);
@@ -963,10 +1144,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public String outputAnnotations(boolean displayTextbox)
   {
     String annotation = new AnnotationFile().printAnnotations(
-            viewport.showAnnotation ? viewport.alignment
-                    .getAlignmentAnnotation() : null, viewport.alignment
-                    .getGroups(),
-            ((Alignment) viewport.alignment).alignmentProperties);
+            viewport.showAnnotation ? viewport.getAlignment()
+                    .getAlignmentAnnotation() : null, viewport
+                    .getAlignment().getGroups(), ((Alignment) viewport
+                    .getAlignment()).alignmentProperties);
 
     if (displayTextbox)
     {
@@ -982,7 +1163,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   private Hashtable getDisplayedFeatureCols()
   {
-    if (alignPanel.getFeatureRenderer() != null)
+    if (alignPanel.getFeatureRenderer() != null
+            && viewport.featuresDisplayed != null)
     {
       FeatureRenderer fr = alignPanel.getFeatureRenderer();
       Hashtable fcols = new Hashtable();
@@ -1002,26 +1184,40 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     String features;
     if (format.equalsIgnoreCase("Jalview"))
     {
-      features = new FeaturesFile().printJalviewFormat(
-              viewport.alignment.getSequencesArray(),
+      features = new FeaturesFile().printJalviewFormat(viewport
+              .getAlignment().getSequencesArray(),
               getDisplayedFeatureCols());
     }
     else
     {
-      features = new FeaturesFile().printGFFFormat(
-              viewport.alignment.getSequencesArray(),
-              getDisplayedFeatureCols());
+      features = new FeaturesFile().printGFFFormat(viewport.getAlignment()
+              .getSequencesArray(), getDisplayedFeatureCols());
     }
 
     if (displayTextbox)
     {
-      CutAndPasteTransfer cap = new CutAndPasteTransfer(false, this);
+      boolean frimport = false;
+      if (features == null || features.equals("No Features Visible"))
+      {
+        features = "# No features visible - paste some and import them here.";
+        frimport = true;
+      }
+
+      CutAndPasteTransfer cap = new CutAndPasteTransfer(frimport, this);
+      if (frimport)
+      {
+        cap.setAnnotationImport();
+      }
       Frame frame = new Frame();
       frame.add(cap);
       jalview.bin.JalviewLite.addFrame(frame, "Features", 600, 500);
-
       cap.setText(features);
     }
+    else
+    {
+      if (features == null)
+        features = "";
+    }
 
     return features;
   }
@@ -1120,19 +1316,30 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public void closeMenuItem_actionPerformed()
   {
     PaintRefresher.RemoveComponent(alignPanel);
-    PaintRefresher.RemoveComponent(alignPanel.seqPanel.seqCanvas);
-    PaintRefresher.RemoveComponent(alignPanel.idPanel.idCanvas);
+    if (alignPanel.seqPanel != null
+            && alignPanel.seqPanel.seqCanvas != null)
+    {
+      PaintRefresher.RemoveComponent(alignPanel.seqPanel.seqCanvas);
+    }
+    if (alignPanel.idPanel != null && alignPanel.idPanel.idCanvas != null)
+    {
+      PaintRefresher.RemoveComponent(alignPanel.idPanel.idCanvas);
+    }
 
     if (PaintRefresher.components.size() == 0 && viewport.applet == null)
     {
       System.exit(0);
     }
-
+    else
+    {
+    }
+    viewport = null;
+    alignPanel = null;
     this.dispose();
   }
 
   /**
-   * DOCUMENT ME!
+   * TODO: JAL-1104
    */
   void updateEditMenuBar()
   {
@@ -1163,6 +1370,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
   }
 
+  /**
+   * TODO: JAL-1104
+   */
   public void addHistoryItem(CommandI command)
   {
     if (command.getSize() > 0)
@@ -1170,12 +1380,12 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       viewport.historyList.push(command);
       viewport.redoList.removeAllElements();
       updateEditMenuBar();
-      viewport.hasHiddenColumns = viewport.colSel.getHiddenColumns() != null;
+      viewport.updateHiddenColumns();
     }
   }
 
   /**
-   * DOCUMENT ME!
+   * TODO: JAL-1104 DOCUMENT ME!
    * 
    * @param e
    *          DOCUMENT ME!
@@ -1192,15 +1402,22 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     command.undoCommand(null);
 
     AlignViewport originalSource = getOriginatingSource(command);
-
-    originalSource.hasHiddenColumns = viewport.colSel.getHiddenColumns() != null;
+    // JBPNote Test
+    if (originalSource != viewport)
+    {
+      System.err
+              .println("Warning: Viewport object mismatch whilst undoing");
+    }
+    originalSource.updateHiddenColumns(); // originalSource.hasHiddenColumns =
+                                          // viewport.getColumnSelection().getHiddenColumns()
+                                          // != null;
     updateEditMenuBar();
-    originalSource.firePropertyChange("alignment", null,
-            originalSource.alignment.getSequences());
+    originalSource.firePropertyChange("alignment", null, originalSource
+            .getAlignment().getSequences());
   }
 
   /**
-   * DOCUMENT ME!
+   * TODO: JAL-1104 DOCUMENT ME!
    * 
    * @param e
    *          DOCUMENT ME!
@@ -1217,11 +1434,19 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     command.doCommand(null);
 
     AlignViewport originalSource = getOriginatingSource(command);
-    originalSource.hasHiddenColumns = viewport.colSel.getHiddenColumns() != null;
+    // JBPNote Test
+    if (originalSource != viewport)
+    {
+      System.err
+              .println("Warning: Viewport object mismatch whilst re-doing");
+    }
+    originalSource.updateHiddenColumns(); // sethasHiddenColumns(); =
+                                          // viewport.getColumnSelection().getHiddenColumns()
+                                          // != null;
 
     updateEditMenuBar();
-    originalSource.firePropertyChange("alignment", null,
-            originalSource.alignment.getSequences());
+    originalSource.firePropertyChange("alignment", null, originalSource
+            .getAlignment().getSequences());
   }
 
   AlignViewport getOriginatingSource(CommandI command)
@@ -1241,7 +1466,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       {
         if (comps.elementAt(i) instanceof AlignmentPanel)
         {
-          if (al == ((AlignmentPanel) comps.elementAt(i)).av.alignment)
+          if (al == ((AlignmentPanel) comps.elementAt(i)).av.getAlignment())
           {
             originalSource = ((AlignmentPanel) comps.elementAt(i)).av;
             break;
@@ -1256,7 +1481,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       // the current view against the closed view first
       if (al != null)
       {
-        PaintRefresher.validateSequences(al, viewport.alignment);
+        PaintRefresher.validateSequences(al, viewport.getAlignment());
       }
 
       originalSource = viewport;
@@ -1272,65 +1497,25 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       return;
     }
-
-    if (up)
-    {
-      for (int i = 1; i < viewport.alignment.getHeight(); i++)
-      {
-        SequenceI seq = viewport.alignment.getSequenceAt(i);
-        if (!sg.getSequences(null).contains(seq))
-        {
-          continue;
-        }
-
-        SequenceI temp = viewport.alignment.getSequenceAt(i - 1);
-        if (sg.getSequences(null).contains(temp))
-        {
-          continue;
-        }
-
-        viewport.alignment.getSequences().setElementAt(temp, i);
-        viewport.alignment.getSequences().setElementAt(seq, i - 1);
-      }
-    }
-    else
-    {
-      for (int i = viewport.alignment.getHeight() - 2; i > -1; i--)
-      {
-        SequenceI seq = viewport.alignment.getSequenceAt(i);
-        if (!sg.getSequences(viewport.hiddenRepSequences).contains(seq))
-        {
-          continue;
-        }
-
-        SequenceI temp = viewport.alignment.getSequenceAt(i + 1);
-        if (sg.getSequences(viewport.hiddenRepSequences).contains(temp))
-        {
-          continue;
-        }
-
-        viewport.alignment.getSequences().setElementAt(temp, i);
-        viewport.alignment.getSequences().setElementAt(seq, i + 1);
-      }
-    }
-
+    viewport.getAlignment().moveSelectedSequencesByOne(sg,
+            up ? null : viewport.getHiddenRepSequences(), up);
     alignPanel.paintAlignment(true);
   }
 
   synchronized void slideSequences(boolean right, int size)
   {
-    Vector sg = new Vector();
+    List<SequenceI> sg = new Vector<SequenceI>();
     if (viewport.cursorMode)
     {
-      sg.addElement(viewport.alignment
-              .getSequenceAt(alignPanel.seqPanel.seqCanvas.cursorY));
+      sg.add(viewport.getAlignment().getSequenceAt(
+              alignPanel.seqPanel.seqCanvas.cursorY));
     }
     else if (viewport.getSelectionGroup() != null
-            && viewport.getSelectionGroup().getSize() != viewport.alignment
-                    .getHeight())
+            && viewport.getSelectionGroup().getSize() != viewport
+                    .getAlignment().getHeight())
     {
       sg = viewport.getSelectionGroup().getSequences(
-              viewport.hiddenRepSequences);
+              viewport.getHiddenRepSequences());
     }
 
     if (sg.size() < 1)
@@ -1338,21 +1523,20 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       return;
     }
 
-    Vector invertGroup = new Vector();
+    Vector<SequenceI> invertGroup = new Vector();
 
-    for (int i = 0; i < viewport.alignment.getHeight(); i++)
+    for (int i = 0; i < viewport.getAlignment().getHeight(); i++)
     {
-      if (!sg.contains(viewport.alignment.getSequenceAt(i)))
-        invertGroup.addElement(viewport.alignment.getSequenceAt(i));
+      if (!sg.contains(viewport.getAlignment().getSequenceAt(i)))
+        invertGroup.addElement(viewport.getAlignment().getSequenceAt(i));
     }
 
-    SequenceI[] seqs1 = new SequenceI[sg.size()];
-    for (int i = 0; i < sg.size(); i++)
-      seqs1[i] = (SequenceI) sg.elementAt(i);
+    SequenceI[] seqs1 = sg.toArray(new SequenceI[sg.size()]);
 
-    SequenceI[] seqs2 = new SequenceI[invertGroup.size()];
+    SequenceI[] seqs2 = invertGroup.toArray(new SequenceI[invertGroup
+            .size()]);
     for (int i = 0; i < invertGroup.size(); i++)
-      seqs2[i] = (SequenceI) invertGroup.elementAt(i);
+      seqs2[i] = invertGroup.elementAt(i);
 
     SlideSequencesCommand ssc;
     if (right)
@@ -1418,14 +1602,14 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     for (int i = 0; i < sg.getSize(); i++)
     {
       SequenceI seq = sg.getSequenceAt(i);
-      int index = viewport.alignment.findIndex(seq);
+      int index = viewport.getAlignment().findIndex(seq);
       orderedSeqs.put(index + "", seq);
     }
 
     int index = 0, startRes, endRes;
     char ch;
 
-    if (viewport.hasHiddenColumns && viewport.getSelectionGroup() != null)
+    if (viewport.hasHiddenColumns() && viewport.getSelectionGroup() != null)
     {
       copiedHiddenColumns = new Vector();
       int hiddenOffset = viewport.getSelectionGroup().getStartRes();
@@ -1572,17 +1756,18 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   {
     for (int i = 0; i < seqs.length; i++)
     {
-      viewport.alignment.addSequence(seqs[i]);
+      viewport.getAlignment().addSequence(seqs[i]);
     }
 
     // !newAlignment
     addHistoryItem(new EditCommand("Add sequences", EditCommand.PASTE,
-            seqs, 0, viewport.alignment.getWidth(), viewport.alignment));
+            seqs, 0, viewport.getAlignment().getWidth(),
+            viewport.getAlignment()));
 
-    viewport.setEndSeq(viewport.alignment.getHeight());
-    viewport.alignment.getWidth();
-    viewport.firePropertyChange("alignment", null,
-            viewport.alignment.getSequences());
+    viewport.setEndSeq(viewport.getAlignment().getHeight());
+    viewport.getAlignment().getWidth();
+    viewport.firePropertyChange("alignment", null, viewport.getAlignment()
+            .getSequences());
 
   }
 
@@ -1610,7 +1795,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
 
     // If the cut affects all sequences, remove highlighted columns
-    if (sg.getSize() == viewport.alignment.getHeight())
+    if (sg.getSize() == viewport.getAlignment().getHeight())
     {
       viewport.getColumnSelection().removeElements(sg.getStartRes(),
               sg.getEndRes() + 1);
@@ -1627,10 +1812,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
      */
     addHistoryItem(new EditCommand("Cut Sequences", EditCommand.CUT, cut,
             sg.getStartRes(), sg.getEndRes() - sg.getStartRes() + 1,
-            viewport.alignment));
+            viewport.getAlignment()));
 
     viewport.setSelectionGroup(null);
-    viewport.alignment.deleteGroup(sg);
+    viewport.getAlignment().deleteGroup(sg);
 
     viewport.firePropertyChange("alignment", null, viewport.getAlignment()
             .getSequences());
@@ -1642,6 +1827,65 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     viewport.sendSelection();
   }
 
+  /**
+   * group consensus toggled
+   * 
+   */
+  protected void showGroupConsensus_actionPerformed()
+  {
+    viewport.setShowGroupConsensus(showGroupConsensus.getState());
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+
+  }
+
+  /**
+   * group conservation toggled.
+   */
+  protected void showGroupConservation_actionPerformed()
+  {
+    viewport.setShowGroupConservation(showGroupConservation.getState());
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+  }
+
+  /*
+   * (non-Javadoc)
+   * 
+   * @see
+   * jalview.jbgui.GAlignFrame#showConsensusHistogram_actionPerformed(java.awt
+   * .event.ActionEvent)
+   */
+  protected void showConsensusHistogram_actionPerformed()
+  {
+    viewport.setShowConsensusHistogram(showConsensusHistogram.getState());
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+  }
+
+  /*
+   * (non-Javadoc)
+   * 
+   * @see
+   * jalview.jbgui.GAlignFrame#showConsensusProfile_actionPerformed(java.awt
+   * .event.ActionEvent)
+   */
+  protected void showSequenceLogo_actionPerformed()
+  {
+    viewport.setShowSequenceLogo(showSequenceLogo.getState());
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+  }
+
+  protected void normSequenceLogo_actionPerformed()
+  {
+    showSequenceLogo.setState(true);
+    viewport.setShowSequenceLogo(true);
+    viewport.setNormaliseSequenceLogo(normSequenceLogo.getState());
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+  }
+
+  protected void applyAutoAnnotationSettings_actionPerformed()
+  {
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+  }
+
   protected void makeGrpsFromSelection_actionPerformed()
   {
     if (viewport.getSelectionGroup() != null)
@@ -1649,9 +1893,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       SequenceGroup[] gps = jalview.analysis.Grouping.makeGroupsFrom(
               viewport.getSequenceSelection(),
               viewport.getAlignmentView(true).getSequenceStrings(
-                      viewport.getGapCharacter()),
-              viewport.alignment.getGroups());
-      viewport.alignment.deleteAllGroups();
+                      viewport.getGapCharacter()), viewport.getAlignment()
+                      .getGroups());
+      viewport.getAlignment().deleteAllGroups();
       viewport.sequenceColours = null;
       viewport.setSelectionGroup(null);
       // set view properties for each group
@@ -1659,24 +1903,22 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       {
         // gps[g].setShowunconserved(viewport.getShowUnconserved());
         gps[g].setshowSequenceLogo(viewport.isShowSequenceLogo());
-        viewport.alignment.addGroup(gps[g]);
+        viewport.getAlignment().addGroup(gps[g]);
         Color col = new Color((int) (Math.random() * 255),
                 (int) (Math.random() * 255), (int) (Math.random() * 255));
         col = col.brighter();
-        for (Enumeration sq = gps[g].getSequences(null).elements(); sq
-                .hasMoreElements(); viewport.setSequenceColour(
-                (SequenceI) sq.nextElement(), col))
-          ;
+        for (SequenceI sq : gps[g].getSequences(null))
+          viewport.setSequenceColour(sq, col);
       }
       PaintRefresher.Refresh(this, viewport.getSequenceSetId());
-      // alignPanel.updateAnnotation();
+      alignPanel.updateAnnotation();
       alignPanel.paintAlignment(true);
     }
   }
 
   protected void deleteGroups_actionPerformed()
   {
-    viewport.alignment.deleteAllGroups();
+    viewport.getAlignment().deleteAllGroups();
     viewport.sequenceColours = null;
     viewport.setSelectionGroup(null);
 
@@ -1690,7 +1932,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       sg.addSequence(viewport.getAlignment().getSequenceAt(i), false);
     }
-    sg.setEndRes(viewport.alignment.getWidth() - 1);
+    sg.setEndRes(viewport.getAlignment().getWidth() - 1);
     viewport.setSelectionGroup(sg);
     alignPanel.paintAlignment(true);
     PaintRefresher.Refresh(alignPanel, viewport.getSequenceSetId());
@@ -1754,11 +1996,11 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       if (viewport.getSelectionGroup() != null)
       {
         seqs = viewport.getSelectionGroup().getSequencesAsArray(
-                viewport.hiddenRepSequences);
+                viewport.getHiddenRepSequences());
       }
       else
       {
-        seqs = viewport.alignment.getSequencesArray();
+        seqs = viewport.getAlignment().getSequencesArray();
       }
 
       TrimRegionCommand trimRegion;
@@ -1766,32 +2008,28 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       {
         trimRegion = new TrimRegionCommand("Remove Left",
                 TrimRegionCommand.TRIM_LEFT, seqs, column,
-                viewport.alignment, viewport.colSel,
-                viewport.selectionGroup);
+                viewport.getAlignment(), viewport.getColumnSelection(),
+                viewport.getSelectionGroup());
         viewport.setStartRes(0);
       }
       else
       {
         trimRegion = new TrimRegionCommand("Remove Right",
                 TrimRegionCommand.TRIM_RIGHT, seqs, column,
-                viewport.alignment, viewport.colSel,
-                viewport.selectionGroup);
+                viewport.getAlignment(), viewport.getColumnSelection(),
+                viewport.getSelectionGroup());
       }
 
       statusBar.setText("Removed " + trimRegion.getSize() + " columns.");
 
       addHistoryItem(trimRegion);
 
-      Vector groups = viewport.alignment.getGroups();
-
-      for (int i = 0; i < groups.size(); i++)
+      for (SequenceGroup sg : viewport.getAlignment().getGroups())
       {
-        SequenceGroup sg = (SequenceGroup) groups.elementAt(i);
-
         if ((trimLeft && !sg.adjustForRemoveLeft(column))
                 || (!trimLeft && !sg.adjustForRemoveRight(column)))
         {
-          viewport.alignment.deleteGroup(sg);
+          viewport.getAlignment().deleteGroup(sg);
         }
       }
 
@@ -1802,23 +2040,24 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   public void removeGappedColumnMenuItem_actionPerformed()
   {
-    int start = 0, end = viewport.alignment.getWidth() - 1;
+    int start = 0, end = viewport.getAlignment().getWidth() - 1;
 
     SequenceI[] seqs;
     if (viewport.getSelectionGroup() != null)
     {
       seqs = viewport.getSelectionGroup().getSequencesAsArray(
-              viewport.hiddenRepSequences);
+              viewport.getHiddenRepSequences());
       start = viewport.getSelectionGroup().getStartRes();
       end = viewport.getSelectionGroup().getEndRes();
     }
     else
     {
-      seqs = viewport.alignment.getSequencesArray();
+      seqs = viewport.getAlignment().getSequencesArray();
     }
 
     RemoveGapColCommand removeGapCols = new RemoveGapColCommand(
-            "Remove Gapped Columns", seqs, start, end, viewport.alignment);
+            "Remove Gapped Columns", seqs, start, end,
+            viewport.getAlignment());
 
     addHistoryItem(removeGapCols);
 
@@ -1827,7 +2066,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
     // This is to maintain viewport position on first residue
     // of first sequence
-    SequenceI seq = viewport.alignment.getSequenceAt(0);
+    SequenceI seq = viewport.getAlignment().getSequenceAt(0);
     int startRes = seq.findPosition(viewport.startRes);
     // ShiftList shifts;
     // viewport.getAlignment().removeGaps(shifts=new ShiftList());
@@ -1842,28 +2081,28 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   public void removeAllGapsMenuItem_actionPerformed()
   {
-    int start = 0, end = viewport.alignment.getWidth() - 1;
+    int start = 0, end = viewport.getAlignment().getWidth() - 1;
 
     SequenceI[] seqs;
     if (viewport.getSelectionGroup() != null)
     {
       seqs = viewport.getSelectionGroup().getSequencesAsArray(
-              viewport.hiddenRepSequences);
+              viewport.getHiddenRepSequences());
       start = viewport.getSelectionGroup().getStartRes();
       end = viewport.getSelectionGroup().getEndRes();
     }
     else
     {
-      seqs = viewport.alignment.getSequencesArray();
+      seqs = viewport.getAlignment().getSequencesArray();
     }
 
     // This is to maintain viewport position on first residue
     // of first sequence
-    SequenceI seq = viewport.alignment.getSequenceAt(0);
+    SequenceI seq = viewport.getAlignment().getSequenceAt(0);
     int startRes = seq.findPosition(viewport.startRes);
 
     addHistoryItem(new RemoveGapsCommand("Remove Gaps", seqs, start, end,
-            viewport.alignment));
+            viewport.getAlignment()));
 
     viewport.setStartRes(seq.findIndex(startRes) - 1);
 
@@ -1886,30 +2125,31 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public AlignFrame newView(String viewtitle)
   {
     AlignmentI newal;
-    if (viewport.hasHiddenRows)
+    if (viewport.hasHiddenRows())
     {
       newal = new Alignment(viewport.getAlignment().getHiddenSequences()
               .getFullAlignment().getSequencesArray());
     }
     else
     {
-      newal = new Alignment(viewport.alignment.getSequencesArray());
+      newal = new Alignment(viewport.getAlignment().getSequencesArray());
     }
 
-    if (viewport.alignment.getAlignmentAnnotation() != null)
+    if (viewport.getAlignment().getAlignmentAnnotation() != null)
     {
-      for (int i = 0; i < viewport.alignment.getAlignmentAnnotation().length; i++)
+      for (int i = 0; i < viewport.getAlignment().getAlignmentAnnotation().length; i++)
       {
-        if (!viewport.alignment.getAlignmentAnnotation()[i].autoCalculated)
+        if (!viewport.getAlignment().getAlignmentAnnotation()[i].autoCalculated)
         {
-          newal.addAnnotation(viewport.alignment.getAlignmentAnnotation()[i]);
+          newal.addAnnotation(viewport.getAlignment()
+                  .getAlignmentAnnotation()[i]);
         }
       }
     }
 
     AlignFrame newaf = new AlignFrame(newal, viewport.applet, "", false);
 
-    newaf.viewport.sequenceSetID = alignPanel.av.getSequenceSetId();
+    newaf.viewport.setSequenceSetId(alignPanel.av.getSequenceSetId());
     PaintRefresher.Register(alignPanel, alignPanel.av.getSequenceSetId());
     PaintRefresher.Register(newaf.alignPanel,
             newaf.alignPanel.av.getSequenceSetId());
@@ -2054,11 +2294,16 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
             overview.getPreferredSize().height + 50);
 
     frame.pack();
+    final AlignmentPanel ap = alignPanel;
     frame.addWindowListener(new WindowAdapter()
     {
+      @Override
       public void windowClosing(WindowEvent e)
       {
-        alignPanel.setOverviewPanel(null);
+        if (ap != null)
+        {
+          ap.setOverviewPanel(null);
+        }
       };
     });
 
@@ -2089,13 +2334,13 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       if (viewport.getConservationSelected())
       {
 
-        Alignment al = (Alignment) viewport.alignment;
+        Alignment al = (Alignment) viewport.getAlignment();
         Conservation c = new Conservation("All",
                 ResidueProperties.propHash, 3, al.getSequences(), 0,
                 al.getWidth() - 1);
 
         c.calculate();
-        c.verdict(false, viewport.ConsPercGaps);
+        c.verdict(false, viewport.getConsPercGaps());
 
         cs.setConservation(c);
 
@@ -2108,82 +2353,19 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
         cs.setConservation(null);
       }
 
-      cs.setConsensus(viewport.hconsensus);
+      cs.setConsensus(viewport.getSequenceConsensusHash());
 
     }
     viewport.setGlobalColourScheme(cs);
 
-    if (viewport.getColourAppliesToAllGroups())
-    {
-      Vector groups = viewport.alignment.getGroups();
-      for (int i = 0; i < groups.size(); i++)
-      {
-        SequenceGroup sg = (SequenceGroup) groups.elementAt(i);
-
-        if (cs == null)
-        {
-          sg.cs = null;
-          continue;
-        }
-        if (cs instanceof ClustalxColourScheme)
-        {
-          sg.cs = new ClustalxColourScheme(
-                  sg.getSequences(viewport.hiddenRepSequences),
-                  sg.getWidth());
-        }
-        else
-        {
-          try
-          {
-            sg.cs = (ColourSchemeI) cs.getClass().newInstance();
-          } catch (Exception ex)
-          {
-            ex.printStackTrace();
-            sg.cs = cs;
-          }
-        }
-
-        if (viewport.getAbovePIDThreshold()
-                || cs instanceof PIDColourScheme
-                || cs instanceof Blosum62ColourScheme)
-        {
-          sg.cs.setThreshold(threshold, viewport.getIgnoreGapsConsensus());
-          sg.cs.setConsensus(AAFrequency.calculate(
-                  sg.getSequences(viewport.hiddenRepSequences), 0,
-                  sg.getWidth()));
-        }
-        else
-        {
-          sg.cs.setThreshold(0, viewport.getIgnoreGapsConsensus());
-        }
-
-        if (viewport.getConservationSelected())
-        {
-          Conservation c = new Conservation("Group",
-                  ResidueProperties.propHash, 3,
-                  sg.getSequences(viewport.hiddenRepSequences), 0,
-                  viewport.alignment.getWidth() - 1);
-          c.calculate();
-          c.verdict(false, viewport.ConsPercGaps);
-          sg.cs.setConservation(c);
-        }
-        else
-        {
-          sg.cs.setConservation(null);
-          sg.cs.setThreshold(0, viewport.getIgnoreGapsConsensus());
-        }
-
-      }
-    }
-
     if (alignPanel.getOverviewPanel() != null)
     {
       alignPanel.getOverviewPanel().updateOverviewImage();
     }
 
     jalview.structure.StructureSelectionManager
-            .getStructureSelectionManager().sequenceColoursChanged(
-                    alignPanel);
+            .getStructureSelectionManager(viewport.applet)
+            .sequenceColoursChanged(alignPanel);
 
     alignPanel.paintAlignment(true);
   }
@@ -2191,7 +2373,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   protected void modifyPID_actionPerformed()
   {
     if (viewport.getAbovePIDThreshold()
-            && viewport.globalColourScheme != null)
+            && viewport.getGlobalColourScheme() != null)
     {
       SliderPanel.setPIDSliderSource(alignPanel,
               viewport.getGlobalColourScheme(), "Background");
@@ -2202,10 +2384,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   protected void modifyConservation_actionPerformed()
   {
     if (viewport.getConservationSelected()
-            && viewport.globalColourScheme != null)
+            && viewport.getGlobalColourScheme() != null)
     {
       SliderPanel.setConservationSlider(alignPanel,
-              viewport.globalColourScheme, "Background");
+              viewport.getGlobalColourScheme(), "Background");
       SliderPanel.showConservationSlider();
     }
   }
@@ -2241,7 +2423,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
             .getAlignment().getSequenceAt(0), null);
 
     addHistoryItem(new OrderCommand("Pairwise Sort", oldOrder,
-            viewport.alignment));
+            viewport.getAlignment()));
     alignPanel.paintAlignment(true);
   }
 
@@ -2249,7 +2431,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   {
     SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
     AlignmentSorter.sortByID(viewport.getAlignment());
-    addHistoryItem(new OrderCommand("ID Sort", oldOrder, viewport.alignment));
+    addHistoryItem(new OrderCommand("ID Sort", oldOrder,
+            viewport.getAlignment()));
     alignPanel.paintAlignment(true);
   }
 
@@ -2258,7 +2441,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
     AlignmentSorter.sortByLength(viewport.getAlignment());
     addHistoryItem(new OrderCommand("Length Sort", oldOrder,
-            viewport.alignment));
+            viewport.getAlignment()));
     alignPanel.paintAlignment(true);
   }
 
@@ -2267,7 +2450,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
     AlignmentSorter.sortByGroup(viewport.getAlignment());
     addHistoryItem(new OrderCommand("Group Sort", oldOrder,
-            viewport.alignment));
+            viewport.getAlignment()));
     alignPanel.paintAlignment(true);
 
   }
@@ -2292,7 +2475,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public void PCAMenuItem_actionPerformed()
   {
     // are the sequences aligned?
-    if (!viewport.alignment.isAligned(false))
+    if (!viewport.getAlignment().isAligned(false))
     {
       SequenceI current;
       int Width = viewport.getAlignment().getWidth();
@@ -2349,7 +2532,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   void NewTreePanel(String type, String pwType, String title)
   {
     // are the sequences aligned?
-    if (!viewport.alignment.isAligned(false))
+    if (!viewport.getAlignment().isAligned(false))
     {
       SequenceI current;
       int Width = viewport.getAlignment().getWidth();
@@ -2369,10 +2552,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
     if ((viewport.getSelectionGroup() != null && viewport
             .getSelectionGroup().getSize() > 1)
-            || (viewport.getSelectionGroup() == null && viewport.alignment
-                    .getHeight() > 1))
+            || (viewport.getAlignment().getHeight() > 1))
     {
-      final TreePanel tp = new TreePanel(viewport, type, pwType);
+      final TreePanel tp = new TreePanel(alignPanel, type, pwType);
 
       addTreeMenuItem(tp, title);
 
@@ -2392,7 +2574,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   public void loadTree(jalview.io.NewickFile tree, String treeFile)
   {
-    TreePanel tp = new TreePanel(viewport, treeFile, "From File - ", tree);
+    TreePanel tp = new TreePanel(alignPanel, treeFile, "From File - ", tree);
     jalview.bin.JalviewLite.addFrame(tp, treeFile, 600, 500);
     addTreeMenuItem(tp, treeFile);
   }
@@ -2413,7 +2595,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     // addHistoryItem(new HistoryItem("Sort", viewport.alignment,
     // HistoryItem.SORT));
     addHistoryItem(new OrderCommand("Order by " + title, oldOrder,
-            viewport.alignment));
+            viewport.getAlignment()));
     alignPanel.paintAlignment(true);
   }
 
@@ -2431,6 +2613,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     sortByTreeMenu.add(item);
     item.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent evt)
       {
         sortByTree(treePanel, title); // treePanel.getTitle());
@@ -2439,6 +2622,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
     treePanel.addWindowListener(new WindowAdapter()
     {
+      @Override
       public void windowOpened(WindowEvent e)
       {
         if (viewport.sortByTree)
@@ -2448,6 +2632,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
         super.windowOpened(e);
       }
 
+      @Override
       public void windowClosing(WindowEvent e)
       {
         sortByTreeMenu.remove(item);
@@ -2455,9 +2640,27 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     });
   }
 
+  public boolean sortBy(AlignmentOrder alorder, String undoname)
+  {
+    SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
+    if (viewport.applet.debug)
+    {
+      System.err.println("Sorting " + alorder.getOrder().size()
+              + " in alignment '" + getTitle() + "'");
+    }
+    AlignmentSorter.sortBy(viewport.getAlignment(), alorder);
+    if (undoname != null)
+    {
+      addHistoryItem(new OrderCommand(undoname, oldOrder,
+              viewport.getAlignment()));
+    }
+    alignPanel.paintAlignment(true);
+    return true;
+  }
+
   protected void documentation_actionPerformed()
   {
-    showURL("http://www.jalview.org/help.html", "HELP");
+    alignPanel.av.applet.openJalviewHelpUrl();
   }
 
   protected void about_actionPerformed()
@@ -2475,6 +2678,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
         this.builddate = builddate;
       }
 
+      @Override
       public void paint(Graphics g)
       {
         g.setColor(Color.white);
@@ -2492,9 +2696,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
         g.drawString("Build date: " + builddate, x, y += fh);
         g.setFont(new Font("Helvetica", Font.PLAIN, 12));
         g.drawString(
-                "Authors:  Jim Procter, Andrew Waterhouse, Michele Clamp, James Cuff, Steve Searle,",
+                "Authors:  Jim Procter, Andrew Waterhouse, Jan Engelhardt, Lauren Lui,",
                 x, y += fh * 1.5);
-        g.drawString("David Martin & Geoff Barton.", x + 50, y += fh);
+        g.drawString("Michele Clamp, James Cuff, Steve Searle, David Martin & Geoff Barton.", x + 50, y += fh+8);
         g.drawString(
                 "Development managed by The Barton Group, University of Dundee, Scotland, UK.",
                 x, y += fh);
@@ -2528,44 +2732,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
     else
     {
-      try
-      {
-        if (url.indexOf(":") == -1)
-        {
-          // TODO: verify (Bas Vroling bug) prepend codebase or server URL to
-          // form valid URL
-          if (url.indexOf("/") == 0)
-          {
-            String codebase = viewport.applet.getCodeBase().toString();
-            url = codebase.substring(0, codebase.length()
-                    - viewport.applet.getCodeBase().getFile().length())
-                    + url;
-          }
-          else
-          {
-            url = viewport.applet.getCodeBase() + url;
-          }
-          System.out.println("Show url (prepended codebase): " + url);
-        }
-        else
-        {
-          System.out.println("Show url: " + url);
-        }
-        if (url.indexOf("javascript:") == 0)
-        {
-          // no target for the javascript context
-          viewport.applet.getAppletContext().showDocument(
-                  new java.net.URL(url));
-        }
-        else
-        {
-          viewport.applet.getAppletContext().showDocument(
-                  new java.net.URL(url), target);
-        }
-      } catch (Exception ex)
-      {
-        ex.printStackTrace();
-      }
+      viewport.applet.showURL(url, target);
     }
   }
 
@@ -2654,12 +2821,18 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   MenuItem buriedColour = new MenuItem();
 
+  MenuItem purinePyrimidineColour = new MenuItem();
+
+  MenuItem RNAHelixColour = new MenuItem();
+
   MenuItem userDefinedColour = new MenuItem();
 
   MenuItem PIDColour = new MenuItem();
 
   MenuItem BLOSUM62Colour = new MenuItem();
 
+  MenuItem tcoffeeColour = new MenuItem();
+
   MenuItem njTreeBlosumMenuItem = new MenuItem();
 
   MenuItem avDistanceTreeBlosumMenuItem = new MenuItem();
@@ -2725,6 +2898,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   CheckboxMenuItem autoCalculate = new CheckboxMenuItem(
           "Autocalculate Consensus", true);
 
+  CheckboxMenuItem sortByTree = new CheckboxMenuItem(
+          "Sort Alignment With New Tree", true);
+
   Menu sortByTreeMenu = new Menu();
 
   Menu sort = new Menu();
@@ -2745,6 +2921,20 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   CheckboxMenuItem followMouseOverFlag = new CheckboxMenuItem();
 
+  Menu autoAnnMenu = new Menu();
+
+  CheckboxMenuItem showSequenceLogo = new CheckboxMenuItem();
+
+  CheckboxMenuItem applyAutoAnnotationSettings = new CheckboxMenuItem();
+
+  CheckboxMenuItem showConsensusHistogram = new CheckboxMenuItem();
+
+  CheckboxMenuItem showGroupConsensus = new CheckboxMenuItem();
+
+  CheckboxMenuItem showGroupConservation = new CheckboxMenuItem();
+
+  CheckboxMenuItem normSequenceLogo = new CheckboxMenuItem();
+
   private void jbInit() throws Exception
   {
 
@@ -2761,6 +2951,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
       item.addActionListener(new java.awt.event.ActionListener()
       {
+        @Override
         public void actionPerformed(ActionEvent e)
         {
           outputText_actionPerformed(e);
@@ -2833,12 +3024,20 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     turnColour.addActionListener(this);
     buriedColour.setLabel("Buried Index");
     buriedColour.addActionListener(this);
+    purinePyrimidineColour.setLabel("Purine/Pyrimidine");
+    purinePyrimidineColour.addActionListener(this);
+    RNAHelixColour.setLabel("by RNA Helices");
+    RNAHelixColour.addActionListener(this);
     userDefinedColour.setLabel("User Defined...");
     userDefinedColour.addActionListener(this);
     PIDColour.setLabel("Percentage Identity");
     PIDColour.addActionListener(this);
     BLOSUM62Colour.setLabel("BLOSUM62 Score");
     BLOSUM62Colour.addActionListener(this);
+    tcoffeeColour.setLabel("T-Coffee Scores");
+    tcoffeeColour.setEnabled(false); // it will enabled only if a score file is
+                                     // provided
+    tcoffeeColour.addActionListener(this);
     avDistanceTreeBlosumMenuItem
             .setLabel("Average Distance Using BLOSUM62");
     avDistanceTreeBlosumMenuItem.addActionListener(this);
@@ -2914,6 +3113,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     sort.setLabel("Sort");
     calculate.setLabel("Calculate Tree");
     autoCalculate.addItemListener(this);
+    sortByTree.addItemListener(this);
     inputText.setLabel("Input from textbox");
     inputText.addActionListener(this);
     centreColumnLabelFlag.setLabel("Centre column labels");
@@ -2947,6 +3147,15 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     hideAllButSelection.setLabel("All but Selected Region (Shift+Ctrl+H)");
     hideAllSelection.setLabel("Selected Region");
     showAllHidden.setLabel("All Sequences and Columns");
+    showGroupConsensus.setLabel("Group Consensus");
+    showGroupConservation.setLabel("Group Conservation");
+    showConsensusHistogram.setLabel("Show Consensus Histogram");
+    showSequenceLogo.setLabel("Show Consensus Logo");
+    normSequenceLogo.setLabel("Normalise Consensus Logo");
+    applyAutoAnnotationSettings.setLabel("Apply to all groups");
+    applyAutoAnnotationSettings.setState(true);
+    autoAnnMenu.setLabel("Autocalculated Annotation");
+
     invertColSel.addActionListener(this);
     showColumns.addActionListener(this);
     showSeqs.addActionListener(this);
@@ -2955,6 +3164,13 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     hideAllButSelection.addActionListener(this);
     hideAllSelection.addActionListener(this);
     showAllHidden.addActionListener(this);
+    showGroupConsensus.addItemListener(this);
+    showGroupConservation.addItemListener(this);
+    showConsensusHistogram.addItemListener(this);
+    showSequenceLogo.addItemListener(this);
+    normSequenceLogo.addItemListener(this);
+
+    applyAutoAnnotationSettings.addItemListener(this);
     formatMenu.setLabel("Format");
     selectMenu.setLabel("Select");
     newView.setLabel("New View");
@@ -3004,6 +3220,14 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     viewMenu.addSeparator();
     viewMenu.add(followMouseOverFlag);
     viewMenu.add(annotationPanelMenuItem);
+    autoAnnMenu.add(applyAutoAnnotationSettings);
+    autoAnnMenu.add(showConsensusHistogram);
+    autoAnnMenu.add(showSequenceLogo);
+    autoAnnMenu.add(normSequenceLogo);
+    autoAnnMenu.addSeparator();
+    autoAnnMenu.add(showGroupConservation);
+    autoAnnMenu.add(showGroupConsensus);
+    viewMenu.add(autoAnnMenu);
     viewMenu.addSeparator();
     viewMenu.add(sequenceFeatures);
     viewMenu.add(featureSettings);
@@ -3025,6 +3249,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     colourMenu.add(turnColour);
     colourMenu.add(buriedColour);
     colourMenu.add(nucleotideColour);
+    colourMenu.add(purinePyrimidineColour);
+    colourMenu.add(tcoffeeColour);
     colourMenu.add(userDefinedColour);
     colourMenu.addSeparator();
     colourMenu.add(conservationMenuItem);
@@ -3032,12 +3258,14 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     colourMenu.add(abovePIDThreshold);
     colourMenu.add(modifyPID);
     colourMenu.add(annotationColour);
+    colourMenu.add(RNAHelixColour);
     calculateMenu.add(sort);
     calculateMenu.add(calculate);
     calculateMenu.addSeparator();
     calculateMenu.add(pairwiseAlignmentMenuItem);
     calculateMenu.add(PCAMenuItem);
     calculateMenu.add(autoCalculate);
+    calculateMenu.add(sortByTree);
     this.add(statusBar, BorderLayout.SOUTH);
     pasteMenu.add(pasteNew);
     pasteMenu.add(pasteThis);
@@ -3146,14 +3374,16 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       final AlignFrame me = this;
       viewport.applet.addFocusListener(new FocusListener()
       {
-        
+
         @Override
         public void focusLost(FocusEvent e)
         {
-          if (me.viewport.applet.currentAlignFrame==me) {
-                  me.viewport.applet.currentAlignFrame = null;
-        }}
-        
+          if (me.viewport.applet.currentAlignFrame == me)
+          {
+            me.viewport.applet.currentAlignFrame = null;
+          }
+        }
+
         @Override
         public void focusGained(FocusEvent e)
         {
@@ -3246,12 +3476,26 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     // TODO: search for a jmv that involves viewer
     if (jmv == null)
     { // create a new viewer/jalview binding.
-      jmv = new ExtJmol(viewer, alignPanel, new SequenceI[][] {seqs});
+      jmv = new ExtJmol(viewer, alignPanel, new SequenceI[][]
+      { seqs });
     }
     return jmv;
 
   }
 
+  /**
+   * bind a pdb file to a sequence in the current view
+   * 
+   * @param sequenceId
+   *          - sequenceId within the dataset.
+   * @param pdbEntryString
+   *          - the short name for the PDB file
+   * @param pdbFile
+   *          - pdb file - either a URL or a valid PDB file.
+   * @return true if binding was as success TODO: consider making an exception
+   *         structure for indicating when PDB parsing or sequenceId location
+   *         fails.
+   */
   public boolean addPdbFile(String sequenceId, String pdbEntryString,
           String pdbFile)
   {
@@ -3356,6 +3600,17 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
         return;
       }
     }
+    if (applet.useXtrnalSviewer)
+    {
+      // register the association(s) and quit, don't create any windows.
+      if (StructureSelectionManager.getStructureSelectionManager(applet)
+              .setMapping(seqs, chains, pdb.getFile(), protocol) == null)
+      {
+        System.err.println("Failed to map " + pdb.getFile() + " ("
+                + protocol + ") to any sequences");
+      }
+      return;
+    }
     if (applet.isAlignPdbStructures() && applet.jmolAvailable)
     {
       // can only do alignments with Jmol
@@ -3404,12 +3659,96 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   }
 
   /**
-   * modify the current selection, providing the user has not made a selection already.
-   * @param sel - sequences from this alignment 
-   * @param csel - columns to be selected on the alignment
+   * modify the current selection, providing the user has not made a selection
+   * already.
+   * 
+   * @param sel
+   *          - sequences from this alignment
+   * @param csel
+   *          - columns to be selected on the alignment
    */
   public void select(SequenceGroup sel, ColumnSelection csel)
   {
     alignPanel.seqPanel.selection(sel, csel, null);
   }
+
+  public void scrollTo(int row, int column)
+  {
+    alignPanel.seqPanel.scrollTo(row, column);
+  }
+
+  public void scrollToRow(int row)
+  {
+    alignPanel.seqPanel.scrollToRow(row);
+  }
+
+  public void scrollToColumn(int column)
+  {
+    alignPanel.seqPanel.scrollToColumn(column);
+  }
+
+  /**
+   * @return the alignments unique ID.
+   */
+  public String getSequenceSetId()
+  {
+    return viewport.getSequenceSetId();
+  }
+
+  /**
+   * Load the (T-Coffee) score file from the specified url
+   * 
+   * @param source
+   *          File/URL/T-COFFEE score file contents
+   * @throws IOException
+   * @return true if alignment was annotated with data from source
+   */
+  public boolean loadScoreFile(String source) throws IOException
+  {
+
+    TCoffeeScoreFile file = new TCoffeeScoreFile(source,
+            AppletFormatAdapter.checkProtocol(source));
+    if (!file.isValid())
+    {
+      // TODO: raise dialog for gui
+      System.err.println("Problems parsing T-Coffee scores: "
+              + file.getWarningMessage());
+      System.err.println("Origin was:\n" + source);
+      return false;
+    }
+
+    /*
+     * check that the score matrix matches the alignment dimensions
+     */
+    AlignmentI aln;
+    if ((aln = viewport.getAlignment()) != null
+            && (aln.getHeight() != file.getHeight() || aln.getWidth() != file
+                    .getWidth()))
+    {
+      // TODO: raise a dialog box here rather than bomb out.
+      System.err
+              .println("The scores matrix does not match the alignment dimensions");
+
+    }
+
+    // TODO add parameter to indicate if matching should be done
+    if (file.annotateAlignment(alignPanel.getAlignment(), false))
+    {
+      alignPanel.fontChanged();
+      tcoffeeColour.setEnabled(true);
+      // switch to this color
+      changeColour(new TCoffeeColourScheme(alignPanel.getAlignment()));
+      return true;
+    }
+    else
+    {
+      System.err.println("Problems resolving T-Coffee scores:");
+      if (file.getWarningMessage() != null)
+      {
+        System.err.println(file.getWarningMessage());
+      }
+    }
+    return false;
+  }
+
 }