JAL-1432 updated copyright notices
[jalview.git] / src / jalview / appletgui / AlignFrame.java
old mode 100755 (executable)
new mode 100644 (file)
index 1bfc475..ab99e06
@@ -1,35 +1,94 @@
 /*
- * Jalview - A Sequence Alignment Editor and Viewer (Development Version 2.4.1)
- * Copyright (C) 2009 AM Waterhouse, J Procter, G Barton, M Clamp, S Searle
+ * Jalview - A Sequence Alignment Editor and Viewer (Version 2.8.0b1)
+ * Copyright (C) 2014 The Jalview Authors
  * 
- * This program is free software; you can redistribute it and/or
- * modify it under the terms of the GNU General Public License
- * as published by the Free Software Foundation; either version 2
- * of the License, or (at your option) any later version.
+ * This file is part of Jalview.
  * 
- * This program is distributed in the hope that it will be useful,
- * but WITHOUT ANY WARRANTY; without even the implied warranty of
- * MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
- * GNU General Public License for more details.
+ * Jalview is free software: you can redistribute it and/or
+ * modify it under the terms of the GNU General Public License 
+ * as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
+ *  
+ * Jalview is distributed in the hope that it will be useful, but 
+ * WITHOUT ANY WARRANTY; without even the implied warranty 
+ * of MERCHANTABILITY or FITNESS FOR A PARTICULAR 
+ * PURPOSE.  See the GNU General Public License for more details.
  * 
- * You should have received a copy of the GNU General Public License
- * along with this program; if not, write to the Free Software
- * Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA  02110-1301, USA
+ * You should have received a copy of the GNU General Public License along with Jalview.  If not, see <http://www.gnu.org/licenses/>.
+ * The Jalview Authors are detailed in the 'AUTHORS' file.
  */
 package jalview.appletgui;
 
-import java.io.*;
-import java.net.*;
-import java.util.*;
-
-import java.awt.*;
-import java.awt.event.*;
-
-import jalview.analysis.*;
-import jalview.commands.*;
-import jalview.datamodel.*;
-import jalview.io.*;
-import jalview.schemes.*;
+import jalview.analysis.AlignmentSorter;
+import jalview.analysis.Conservation;
+import jalview.api.SequenceStructureBinding;
+import jalview.bin.JalviewLite;
+import jalview.commands.CommandI;
+import jalview.commands.EditCommand;
+import jalview.commands.OrderCommand;
+import jalview.commands.RemoveGapColCommand;
+import jalview.commands.RemoveGapsCommand;
+import jalview.commands.SlideSequencesCommand;
+import jalview.commands.TrimRegionCommand;
+import jalview.datamodel.Alignment;
+import jalview.datamodel.AlignmentI;
+import jalview.datamodel.AlignmentOrder;
+import jalview.datamodel.ColumnSelection;
+import jalview.datamodel.PDBEntry;
+import jalview.datamodel.Sequence;
+import jalview.datamodel.SequenceGroup;
+import jalview.datamodel.SequenceI;
+import jalview.io.AnnotationFile;
+import jalview.io.AppletFormatAdapter;
+import jalview.io.FeaturesFile;
+import jalview.io.TCoffeeScoreFile;
+import jalview.schemes.Blosum62ColourScheme;
+import jalview.schemes.BuriedColourScheme;
+import jalview.schemes.ClustalxColourScheme;
+import jalview.schemes.ColourSchemeI;
+import jalview.schemes.HelixColourScheme;
+import jalview.schemes.HydrophobicColourScheme;
+import jalview.schemes.NucleotideColourScheme;
+import jalview.schemes.PIDColourScheme;
+import jalview.schemes.PurinePyrimidineColourScheme;
+import jalview.schemes.RNAHelicesColourChooser;
+import jalview.schemes.ResidueProperties;
+import jalview.schemes.StrandColourScheme;
+import jalview.schemes.TCoffeeColourScheme;
+import jalview.schemes.TaylorColourScheme;
+import jalview.schemes.TurnColourScheme;
+import jalview.schemes.ZappoColourScheme;
+import jalview.structure.StructureSelectionManager;
+
+import java.awt.BorderLayout;
+import java.awt.Canvas;
+import java.awt.CheckboxMenuItem;
+import java.awt.Color;
+import java.awt.Font;
+import java.awt.FontMetrics;
+import java.awt.Frame;
+import java.awt.Graphics;
+import java.awt.Label;
+import java.awt.Menu;
+import java.awt.MenuBar;
+import java.awt.MenuItem;
+import java.awt.event.ActionEvent;
+import java.awt.event.ActionListener;
+import java.awt.event.FocusEvent;
+import java.awt.event.FocusListener;
+import java.awt.event.ItemEvent;
+import java.awt.event.ItemListener;
+import java.awt.event.KeyEvent;
+import java.awt.event.KeyListener;
+import java.awt.event.WindowAdapter;
+import java.awt.event.WindowEvent;
+import java.io.IOException;
+import java.net.URL;
+import java.net.URLEncoder;
+import java.util.Enumeration;
+import java.util.Hashtable;
+import java.util.List;
+import java.util.StringTokenizer;
+import java.util.Vector;
 
 public class AlignFrame extends EmbmenuFrame implements ActionListener,
         ItemListener, KeyListener
@@ -47,7 +106,6 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public AlignFrame(AlignmentI al, jalview.bin.JalviewLite applet,
           String title, boolean embedded)
   {
-
     if (applet != null)
     {
       jalviewServletURL = applet.getParameter("APPLICATION_URL");
@@ -68,7 +126,13 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     viewport.updateConsensus(alignPanel);
 
     annotationPanelMenuItem.setState(viewport.showAnnotation);
-    displayNonconservedMenuItem.setState(viewport.getShowunconserved());
+    displayNonconservedMenuItem.setState(viewport.getShowUnconserved());
+    followMouseOverFlag.setState(viewport.getFollowHighlight());
+    showGroupConsensus.setState(viewport.isShowGroupConsensus());
+    showGroupConservation.setState(viewport.isShowGroupConservation());
+    showConsensusHistogram.setState(viewport.isShowConsensusHistogram());
+    showSequenceLogo.setState(viewport.isShowSequenceLogo());
+    normSequenceLogo.setState(viewport.isNormaliseSequenceLogo());
 
     seqLimits.setState(viewport.showJVSuffix);
 
@@ -85,6 +149,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
         {
           sortPairwiseMenuItem_actionPerformed();
         }
+        else if (param.equalsIgnoreCase("Length"))
+        {
+          sortLengthMenuItem_actionPerformed();
+        }
       }
 
       param = applet.getParameter("wrap");
@@ -121,7 +189,23 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       }
 
     }
-
+    if (viewport.getAlignment().isNucleotide())
+    {
+      viewport.updateStrucConsensus(alignPanel);
+      if (viewport.getAlignment().hasRNAStructure())
+      {
+        RNAHelixColour.setEnabled(true);
+      }
+      else
+      {
+        RNAHelixColour.setEnabled(false);
+      }
+    }
+    else
+    {
+      RNAHelixColour.setEnabled(false);
+      purinePyrimidineColour.setEnabled(false);
+    }
     // Some JVMS send keyevents to Top frame or lowest panel,
     // Havent worked out why yet. So add to both this frame and seqCanvas for
     // now
@@ -130,8 +214,13 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     alignPanel.idPanel.idCanvas.addKeyListener(this);
     alignPanel.scalePanel.addKeyListener(this);
     alignPanel.annotationPanel.addKeyListener(this);
+    alignPanel.annotationPanelHolder.addKeyListener(this);
+    alignPanel.annotationSpaceFillerHolder.addKeyListener(this);
+    alignPanel.alabels.addKeyListener(this);
     createAlignFrameWindow(embedded, title);
-    alignPanel.validate();
+
+    validate();
+    alignPanel.adjustAnnotationHeight();
     alignPanel.paintAlignment(true);
   }
 
@@ -146,22 +235,46 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   }
 
   /**
-   * DOCUMENT ME!
+   * Load a features file onto the alignment
    * 
-   * @param String
-   *                DOCUMENT ME!
+   * @param file
+   *          file URL, content, or other resolvable path
+   * @param type
+   *          is protocol for accessing data referred to by file
    */
 
-  public void parseFeaturesFile(String file, String type)
+  public boolean parseFeaturesFile(String file, String type)
+  {
+    return parseFeaturesFile(file, type, true);
+  }
+
+  /**
+   * Load a features file onto the alignment
+   * 
+   * @param file
+   *          file URL, content, or other resolvable path
+   * @param type
+   *          is protocol for accessing data referred to by file
+   * @param autoenabledisplay
+   *          when true, display features flag will be automatically enabled if
+   *          features are loaded
+   * @return true if data parsed as a features file
+   */
+  public boolean parseFeaturesFile(String file, String type,
+          boolean autoenabledisplay)
   {
+    // TODO: test if importing a features file onto an alignment which already
+    // has features with links overwrites the original links.
+
     Hashtable featureLinks = new Hashtable();
     boolean featuresFile = false;
     try
     {
-      featuresFile = new jalview.io.FeaturesFile(file, type).parse(
-              viewport.alignment, alignPanel.seqPanel.seqCanvas
+      featuresFile = new jalview.io.FeaturesFile(file, type)
+              .parse(viewport.getAlignment(), alignPanel.seqPanel.seqCanvas
                       .getFeatureRenderer().featureColours, featureLinks,
-              true);
+                      true, viewport.applet.getDefaultParameter(
+                              "relaxedidmatch", false));
     } catch (Exception ex)
     {
       ex.printStackTrace();
@@ -173,17 +286,22 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       {
         alignPanel.seqPanel.seqCanvas.getFeatureRenderer().featureLinks = featureLinks;
       }
-      viewport.showSequenceFeatures = true;
-      sequenceFeatures.setState(true);
-      if (viewport.featureSettings!=null)
+      if (autoenabledisplay)
+      {
+        viewport.showSequenceFeatures = true;
+        sequenceFeatures.setState(true);
+      }
+      if (viewport.featureSettings != null)
       {
         viewport.featureSettings.refreshTable();
       }
       alignPanel.paintAlignment(true);
+      statusBar.setText("Successfully added features to alignment.");
     }
-
+    return featuresFile;
   }
 
+  @Override
   public void keyPressed(KeyEvent evt)
   {
     if (viewport.cursorMode
@@ -197,6 +315,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
     case 27: // escape key
       deselectAllSequenceMenuItem_actionPerformed();
+
+      alignPanel.alabels.cancelDrag();
       break;
     case KeyEvent.VK_X:
       if (evt.isControlDown() || evt.isMetaDown())
@@ -340,37 +460,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       boolean toggleSeqs = !evt.isControlDown();
       boolean toggleCols = !evt.isShiftDown();
-      boolean hide = false;
-      SequenceGroup sg = viewport.getSelectionGroup();
-
-      if (toggleSeqs)
-      {
-        if (sg != null && sg.getSize() != viewport.alignment.getHeight())
-        {
-          hide = true;
-          viewport.hideAllSelectedSeqs();
-        }
-        else if (!(toggleCols && viewport.colSel.getSelected().size() > 0))
-        {
-          viewport.showAllHiddenSeqs();
-        }
-      }
-
-      if (toggleCols)
-      {
-        if (viewport.colSel.getSelected().size() > 0)
-        {
-          viewport.hideSelectedColumns();
-          if (!toggleSeqs)
-          {
-            viewport.selectionGroup = sg;
-          }
-        }
-        else if (!hide)
-        {
-          viewport.showAllHiddenColumns();
-        }
-      }
+      toggleHiddenRegions(toggleSeqs, toggleCols);
       break;
     }
 
@@ -444,11 +534,11 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       {
         if (evt.isAltDown())
         {
-          viewport.invertColumnSelection();
+          invertColSel_actionPerformed();
         }
         else
         {
-          this.invertSequenceMenuItem_actionPerformed();
+          invertSequenceMenuItem_actionPerformed();
         }
       }
       break;
@@ -471,14 +561,86 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     alignPanel.paintAlignment(true);
   }
 
+  /**
+   * called by key handler and the hide all/show all menu items
+   * 
+   * @param toggleSeqs
+   * @param toggleCols
+   */
+  private void toggleHiddenRegions(boolean toggleSeqs, boolean toggleCols)
+  {
+    boolean hide = false;
+    SequenceGroup sg = viewport.getSelectionGroup();
+    if (!toggleSeqs && !toggleCols)
+    {
+      // Hide everything by the current selection - this is a hack - we do the
+      // invert and then hide
+      // first check that there will be visible columns after the invert.
+      if ((viewport.getColumnSelection() != null
+              && viewport.getColumnSelection().getSelected() != null && viewport
+              .getColumnSelection().getSelected().size() > 0)
+              || (sg != null && sg.getSize() > 0 && sg.getStartRes() <= sg
+                      .getEndRes()))
+      {
+        // now invert the sequence set, if required - empty selection implies
+        // that no hiding is required.
+        if (sg != null)
+        {
+          invertSequenceMenuItem_actionPerformed();
+          sg = viewport.getSelectionGroup();
+          toggleSeqs = true;
+
+        }
+        viewport.expandColSelection(sg, true);
+        // finally invert the column selection and get the new sequence
+        // selection and indicate it should be hidden.
+        invertColSel_actionPerformed();
+        toggleCols = true;
+      }
+    }
+
+    if (toggleSeqs)
+    {
+      if (sg != null && sg.getSize() != viewport.getAlignment().getHeight())
+      {
+        hide = true;
+        viewport.hideAllSelectedSeqs();
+      }
+      else if (!(toggleCols && viewport.getColumnSelection().getSelected()
+              .size() > 0))
+      {
+        viewport.showAllHiddenSeqs();
+      }
+    }
+
+    if (toggleCols)
+    {
+      if (viewport.getColumnSelection().getSelected().size() > 0)
+      {
+        viewport.hideSelectedColumns();
+        if (!toggleSeqs)
+        {
+          viewport.setSelectionGroup(sg);
+        }
+      }
+      else if (!hide)
+      {
+        viewport.showAllHiddenColumns();
+      }
+    }
+  }
+
+  @Override
   public void keyReleased(KeyEvent evt)
   {
   }
 
+  @Override
   public void keyTyped(KeyEvent evt)
   {
   }
 
+  @Override
   public void itemStateChanged(ItemEvent evt)
   {
     if (evt.getSource() == displayNonconservedMenuItem)
@@ -545,23 +707,52 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
     else if (evt.getSource() == autoCalculate)
     {
-      viewport.autocalculateConsensus = autoCalculate.getState();
+      viewport.autoCalculateConsensus = autoCalculate.getState();
+    }
+    else if (evt.getSource() == sortByTree)
+    {
+      viewport.sortByTree = sortByTree.getState();
     }
     else if (evt.getSource() == this.centreColumnLabelFlag)
     {
       centreColumnLabelFlag_stateChanged();
-    } else if (evt.getSource() == this.followMouseOverFlag)
+    }
+    else if (evt.getSource() == this.followMouseOverFlag)
     {
       mouseOverFlag_stateChanged();
     }
-
+    else if (evt.getSource() == showGroupConsensus)
+    {
+      showGroupConsensus_actionPerformed();
+    }
+    else if (evt.getSource() == showGroupConservation)
+    {
+      showGroupConservation_actionPerformed();
+    }
+    else if (evt.getSource() == showSequenceLogo)
+    {
+      showSequenceLogo_actionPerformed();
+    }
+    else if (evt.getSource() == normSequenceLogo)
+    {
+      normSequenceLogo_actionPerformed();
+    }
+    else if (evt.getSource() == showConsensusHistogram)
+    {
+      showConsensusHistogram_actionPerformed();
+    }
+    else if (evt.getSource() == applyAutoAnnotationSettings)
+    {
+      applyAutoAnnotationSettings_actionPerformed();
+    }
     alignPanel.paintAlignment(true);
   }
 
   private void mouseOverFlag_stateChanged()
   {
     viewport.followHighlight = followMouseOverFlag.getState();
-    // TODO: could kick the scrollTo mechanism to reset view for current searchresults.
+    // TODO: could kick the scrollTo mechanism to reset view for current
+    // searchresults.
   }
 
   private void centreColumnLabelFlag_stateChanged()
@@ -570,6 +761,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     this.alignPanel.annotationPanel.repaint();
   }
 
+  @Override
   public void actionPerformed(ActionEvent evt)
   {
     Object source = evt.getSource();
@@ -650,6 +842,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       delete_actionPerformed();
     }
+    else if (source == grpsFromSelection)
+    {
+      makeGrpsFromSelection_actionPerformed();
+    }
     else if (source == deleteGroups)
     {
       deleteGroups_actionPerformed();
@@ -707,6 +903,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     else if (source == showSeqs)
     {
       viewport.showAllHiddenSeqs();
+      alignPanel.paintAlignment(true);
     }
     else if (source == hideColumns)
     {
@@ -717,6 +914,50 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
             && viewport.getSelectionGroup() != null)
     {
       viewport.hideAllSelectedSeqs();
+      alignPanel.paintAlignment(true);
+    }
+    else if (source == hideAllButSelection)
+    {
+      toggleHiddenRegions(false, false);
+      alignPanel.paintAlignment(true);
+    }
+    else if (source == hideAllSelection)
+    {
+      SequenceGroup sg = viewport.getSelectionGroup();
+      viewport.expandColSelection(sg, false);
+      viewport.hideAllSelectedSeqs();
+      viewport.hideSelectedColumns();
+      alignPanel.paintAlignment(true);
+    }
+    else if (source == showAllHidden)
+    {
+      viewport.showAllHiddenColumns();
+      viewport.showAllHiddenSeqs();
+      alignPanel.paintAlignment(true);
+    }
+    else if (source == showGroupConsensus)
+    {
+      showGroupConsensus_actionPerformed();
+    }
+    else if (source == showGroupConservation)
+    {
+      showGroupConservation_actionPerformed();
+    }
+    else if (source == showSequenceLogo)
+    {
+      showSequenceLogo_actionPerformed();
+    }
+    else if (source == normSequenceLogo)
+    {
+      normSequenceLogo_actionPerformed();
+    }
+    else if (source == showConsensusHistogram)
+    {
+      showConsensusHistogram_actionPerformed();
+    }
+    else if (source == applyAutoAnnotationSettings)
+    {
+      applyAutoAnnotationSettings_actionPerformed();
     }
     else if (source == featureSettings)
     {
@@ -724,38 +965,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
     else if (source == alProperties)
     {
-      StringBuffer contents = new StringBuffer();
-
-      float avg = 0;
-      int min = Integer.MAX_VALUE, max = 0;
-      for (int i = 0; i < viewport.alignment.getHeight(); i++)
-      {
-        int size = viewport.alignment.getSequenceAt(i).getEnd()
-                - viewport.alignment.getSequenceAt(i).getStart();
-        avg += size;
-        if (size > max)
-          max = size;
-        if (size < min)
-          min = size;
-      }
-      avg = avg / (float) viewport.alignment.getHeight();
-
-      contents.append("\nSequences: " + viewport.alignment.getHeight());
-      contents.append("\nMinimum Sequence Length: " + min);
-      contents.append("\nMaximum Sequence Length: " + max);
-      contents.append("\nAverage Length: " + (int) avg);
-
-      if (((Alignment) viewport.alignment).alignmentProperties != null)
-      {
-        Hashtable props = ((Alignment) viewport.alignment).alignmentProperties;
-        Enumeration en = props.keys();
-        while (en.hasMoreElements())
-        {
-          String key = en.nextElement().toString();
-          contents.append("\n" + key + "\t" + props.get(key));
-        }
-      }
-
+      StringBuffer contents = new jalview.io.AlignmentProperties(
+              viewport.getAlignment()).formatAsString();
       CutAndPasteTransfer cap = new CutAndPasteTransfer(false, this);
       cap.setText(contents.toString());
       Frame frame = new Frame();
@@ -774,8 +985,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     else if (source == clustalColour)
     {
       abovePIDThreshold.setState(false);
-      changeColour(new ClustalxColourScheme(viewport.alignment
-              .getSequences(), viewport.alignment.getWidth()));
+      changeColour(new ClustalxColourScheme(viewport.getAlignment(), null));
     }
     else if (source == zappoColour)
     {
@@ -809,6 +1019,14 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       changeColour(new NucleotideColourScheme());
     }
+    else if (source == purinePyrimidineColour)
+    {
+      changeColour(new PurinePyrimidineColourScheme());
+    }
+    else if (source == RNAHelixColour)
+    {
+      new RNAHelicesColourChooser(viewport, alignPanel);
+    }
     else if (source == modifyPID)
     {
       modifyPID_actionPerformed();
@@ -829,6 +1047,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       changeColour(new Blosum62ColourScheme());
     }
+    else if (source == tcoffeeColour)
+    {
+      changeColour(new TCoffeeColourScheme(alignPanel.getAlignment()));
+    }
     else if (source == annotationColour)
     {
       new AnnotationColourChooser(viewport, alignPanel);
@@ -841,6 +1063,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       sortIDMenuItem_actionPerformed();
     }
+    else if (source == sortLengthMenuItem)
+    {
+      sortLengthMenuItem_actionPerformed();
+    }
     else if (source == sortGroupMenuItem)
     {
       sortGroupMenuItem_actionPerformed();
@@ -897,17 +1123,17 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     CutAndPasteTransfer cap = new CutAndPasteTransfer(true, this);
     Frame frame = new Frame();
     frame.add(cap);
-    jalview.bin.JalviewLite.addFrame(frame, "Alignment output - "
-            + e.getActionCommand(), 600, 500);
-    cap.setText(new AppletFormatAdapter().formatSequences(e
-            .getActionCommand(), viewport.getAlignment(),
+    jalview.bin.JalviewLite.addFrame(frame,
+            "Alignment output - " + e.getActionCommand(), 600, 500);
+    cap.setText(new AppletFormatAdapter().formatSequences(
+            e.getActionCommand(), viewport.getAlignment(),
             viewport.showJVSuffix));
   }
 
   public void loadAnnotations()
   {
     CutAndPasteTransfer cap = new CutAndPasteTransfer(true, this);
-    cap.setText("Paste your features / annotations file here.");
+    cap.setText("Paste your features / annotations / T-coffee score file here.");
     cap.setAnnotationImport();
     Frame frame = new Frame();
     frame.add(cap);
@@ -918,10 +1144,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public String outputAnnotations(boolean displayTextbox)
   {
     String annotation = new AnnotationFile().printAnnotations(
-            viewport.showAnnotation ? viewport.alignment
-                    .getAlignmentAnnotation() : null, viewport.alignment
-                    .getGroups(),
-            ((Alignment) viewport.alignment).alignmentProperties);
+            viewport.showAnnotation ? viewport.getAlignment()
+                    .getAlignmentAnnotation() : null, viewport
+                    .getAlignment().getGroups(), ((Alignment) viewport
+                    .getAlignment()).alignmentProperties);
 
     if (displayTextbox)
     {
@@ -937,14 +1163,16 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   private Hashtable getDisplayedFeatureCols()
   {
-    if (alignPanel.getFeatureRenderer()!=null) {
+    if (alignPanel.getFeatureRenderer() != null
+            && viewport.featuresDisplayed != null)
+    {
       FeatureRenderer fr = alignPanel.getFeatureRenderer();
       Hashtable fcols = new Hashtable();
       Enumeration en = viewport.featuresDisplayed.keys();
       while (en.hasMoreElements())
       {
         Object col = en.nextElement();
-        fcols.put(col,fr.featureColours.get(col));
+        fcols.put(col, fr.featureColours.get(col));
       }
       return fcols;
     }
@@ -956,24 +1184,40 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     String features;
     if (format.equalsIgnoreCase("Jalview"))
     {
-      features = new FeaturesFile().printJalviewFormat(viewport.alignment
-              .getSequencesArray(), getDisplayedFeatureCols());
+      features = new FeaturesFile().printJalviewFormat(viewport
+              .getAlignment().getSequencesArray(),
+              getDisplayedFeatureCols());
     }
     else
     {
-      features = new FeaturesFile().printGFFFormat(viewport.alignment
+      features = new FeaturesFile().printGFFFormat(viewport.getAlignment()
               .getSequencesArray(), getDisplayedFeatureCols());
     }
 
     if (displayTextbox)
     {
-      CutAndPasteTransfer cap = new CutAndPasteTransfer(false, this);
+      boolean frimport = false;
+      if (features == null || features.equals("No Features Visible"))
+      {
+        features = "# No features visible - paste some and import them here.";
+        frimport = true;
+      }
+
+      CutAndPasteTransfer cap = new CutAndPasteTransfer(frimport, this);
+      if (frimport)
+      {
+        cap.setAnnotationImport();
+      }
       Frame frame = new Frame();
       frame.add(cap);
       jalview.bin.JalviewLite.addFrame(frame, "Features", 600, 500);
-
       cap.setText(features);
     }
+    else
+    {
+      if (features == null)
+        features = "";
+    }
 
     return features;
   }
@@ -994,9 +1238,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     if (viewport.applet.getParameter("annotations") != null)
     {
       url.append("&annotations=");
-      url
-              .append(appendProtocol(viewport.applet
-                      .getParameter("annotations")));
+      url.append(appendProtocol(viewport.applet.getParameter("annotations")));
     }
 
     if (viewport.applet.getParameter("jnetfile") != null)
@@ -1074,19 +1316,30 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public void closeMenuItem_actionPerformed()
   {
     PaintRefresher.RemoveComponent(alignPanel);
-    PaintRefresher.RemoveComponent(alignPanel.seqPanel.seqCanvas);
-    PaintRefresher.RemoveComponent(alignPanel.idPanel.idCanvas);
+    if (alignPanel.seqPanel != null
+            && alignPanel.seqPanel.seqCanvas != null)
+    {
+      PaintRefresher.RemoveComponent(alignPanel.seqPanel.seqCanvas);
+    }
+    if (alignPanel.idPanel != null && alignPanel.idPanel.idCanvas != null)
+    {
+      PaintRefresher.RemoveComponent(alignPanel.idPanel.idCanvas);
+    }
 
     if (PaintRefresher.components.size() == 0 && viewport.applet == null)
     {
       System.exit(0);
     }
-
+    else
+    {
+    }
+    viewport = null;
+    alignPanel = null;
     this.dispose();
   }
 
   /**
-   * DOCUMENT ME!
+   * TODO: JAL-1104
    */
   void updateEditMenuBar()
   {
@@ -1117,6 +1370,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
   }
 
+  /**
+   * TODO: JAL-1104
+   */
   public void addHistoryItem(CommandI command)
   {
     if (command.getSize() > 0)
@@ -1124,15 +1380,15 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       viewport.historyList.push(command);
       viewport.redoList.removeAllElements();
       updateEditMenuBar();
-      viewport.hasHiddenColumns = viewport.colSel.getHiddenColumns() != null;
+      viewport.updateHiddenColumns();
     }
   }
 
   /**
-   * DOCUMENT ME!
+   * TODO: JAL-1104 DOCUMENT ME!
    * 
    * @param e
-   *                DOCUMENT ME!
+   *          DOCUMENT ME!
    */
   protected void undoMenuItem_actionPerformed()
   {
@@ -1146,18 +1402,25 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     command.undoCommand(null);
 
     AlignViewport originalSource = getOriginatingSource(command);
-
-    originalSource.hasHiddenColumns = viewport.colSel.getHiddenColumns() != null;
+    // JBPNote Test
+    if (originalSource != viewport)
+    {
+      System.err
+              .println("Warning: Viewport object mismatch whilst undoing");
+    }
+    originalSource.updateHiddenColumns(); // originalSource.hasHiddenColumns =
+                                          // viewport.getColumnSelection().getHiddenColumns()
+                                          // != null;
     updateEditMenuBar();
-    originalSource.firePropertyChange("alignment", null,
-            originalSource.alignment.getSequences());
+    originalSource.firePropertyChange("alignment", null, originalSource
+            .getAlignment().getSequences());
   }
 
   /**
-   * DOCUMENT ME!
+   * TODO: JAL-1104 DOCUMENT ME!
    * 
    * @param e
-   *                DOCUMENT ME!
+   *          DOCUMENT ME!
    */
   protected void redoMenuItem_actionPerformed()
   {
@@ -1171,11 +1434,19 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     command.doCommand(null);
 
     AlignViewport originalSource = getOriginatingSource(command);
-    originalSource.hasHiddenColumns = viewport.colSel.getHiddenColumns() != null;
+    // JBPNote Test
+    if (originalSource != viewport)
+    {
+      System.err
+              .println("Warning: Viewport object mismatch whilst re-doing");
+    }
+    originalSource.updateHiddenColumns(); // sethasHiddenColumns(); =
+                                          // viewport.getColumnSelection().getHiddenColumns()
+                                          // != null;
 
     updateEditMenuBar();
-    originalSource.firePropertyChange("alignment", null,
-            originalSource.alignment.getSequences());
+    originalSource.firePropertyChange("alignment", null, originalSource
+            .getAlignment().getSequences());
   }
 
   AlignViewport getOriginatingSource(CommandI command)
@@ -1195,7 +1466,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       {
         if (comps.elementAt(i) instanceof AlignmentPanel)
         {
-          if (al == ((AlignmentPanel) comps.elementAt(i)).av.alignment)
+          if (al == ((AlignmentPanel) comps.elementAt(i)).av.getAlignment())
           {
             originalSource = ((AlignmentPanel) comps.elementAt(i)).av;
             break;
@@ -1210,7 +1481,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       // the current view against the closed view first
       if (al != null)
       {
-        PaintRefresher.validateSequences(al, viewport.alignment);
+        PaintRefresher.validateSequences(al, viewport.getAlignment());
       }
 
       originalSource = viewport;
@@ -1226,65 +1497,25 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       return;
     }
-
-    if (up)
-    {
-      for (int i = 1; i < viewport.alignment.getHeight(); i++)
-      {
-        SequenceI seq = viewport.alignment.getSequenceAt(i);
-        if (!sg.getSequences(null).contains(seq))
-        {
-          continue;
-        }
-
-        SequenceI temp = viewport.alignment.getSequenceAt(i - 1);
-        if (sg.getSequences(null).contains(temp))
-        {
-          continue;
-        }
-
-        viewport.alignment.getSequences().setElementAt(temp, i);
-        viewport.alignment.getSequences().setElementAt(seq, i - 1);
-      }
-    }
-    else
-    {
-      for (int i = viewport.alignment.getHeight() - 2; i > -1; i--)
-      {
-        SequenceI seq = viewport.alignment.getSequenceAt(i);
-        if (!sg.getSequences(viewport.hiddenRepSequences).contains(seq))
-        {
-          continue;
-        }
-
-        SequenceI temp = viewport.alignment.getSequenceAt(i + 1);
-        if (sg.getSequences(viewport.hiddenRepSequences).contains(temp))
-        {
-          continue;
-        }
-
-        viewport.alignment.getSequences().setElementAt(temp, i);
-        viewport.alignment.getSequences().setElementAt(seq, i + 1);
-      }
-    }
-
+    viewport.getAlignment().moveSelectedSequencesByOne(sg,
+            up ? null : viewport.getHiddenRepSequences(), up);
     alignPanel.paintAlignment(true);
   }
 
   synchronized void slideSequences(boolean right, int size)
   {
-    Vector sg = new Vector();
+    List<SequenceI> sg = new Vector<SequenceI>();
     if (viewport.cursorMode)
     {
-      sg.addElement(viewport.alignment
-              .getSequenceAt(alignPanel.seqPanel.seqCanvas.cursorY));
+      sg.add(viewport.getAlignment().getSequenceAt(
+              alignPanel.seqPanel.seqCanvas.cursorY));
     }
     else if (viewport.getSelectionGroup() != null
-            && viewport.getSelectionGroup().getSize() != viewport.alignment
-                    .getHeight())
+            && viewport.getSelectionGroup().getSize() != viewport
+                    .getAlignment().getHeight())
     {
       sg = viewport.getSelectionGroup().getSequences(
-              viewport.hiddenRepSequences);
+              viewport.getHiddenRepSequences());
     }
 
     if (sg.size() < 1)
@@ -1292,21 +1523,20 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       return;
     }
 
-    Vector invertGroup = new Vector();
+    Vector<SequenceI> invertGroup = new Vector();
 
-    for (int i = 0; i < viewport.alignment.getHeight(); i++)
+    for (int i = 0; i < viewport.getAlignment().getHeight(); i++)
     {
-      if (!sg.contains(viewport.alignment.getSequenceAt(i)))
-        invertGroup.addElement(viewport.alignment.getSequenceAt(i));
+      if (!sg.contains(viewport.getAlignment().getSequenceAt(i)))
+        invertGroup.addElement(viewport.getAlignment().getSequenceAt(i));
     }
 
-    SequenceI[] seqs1 = new SequenceI[sg.size()];
-    for (int i = 0; i < sg.size(); i++)
-      seqs1[i] = (SequenceI) sg.elementAt(i);
+    SequenceI[] seqs1 = sg.toArray(new SequenceI[sg.size()]);
 
-    SequenceI[] seqs2 = new SequenceI[invertGroup.size()];
+    SequenceI[] seqs2 = invertGroup.toArray(new SequenceI[invertGroup
+            .size()]);
     for (int i = 0; i < invertGroup.size(); i++)
-      seqs2[i] = (SequenceI) invertGroup.elementAt(i);
+      seqs2[i] = invertGroup.elementAt(i);
 
     SlideSequencesCommand ssc;
     if (right)
@@ -1372,14 +1602,14 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     for (int i = 0; i < sg.getSize(); i++)
     {
       SequenceI seq = sg.getSequenceAt(i);
-      int index = viewport.alignment.findIndex(seq);
+      int index = viewport.getAlignment().findIndex(seq);
       orderedSeqs.put(index + "", seq);
     }
 
     int index = 0, startRes, endRes;
     char ch;
 
-    if (viewport.hasHiddenColumns && viewport.getSelectionGroup() != null)
+    if (viewport.hasHiddenColumns() && viewport.getSelectionGroup() != null)
     {
       copiedHiddenColumns = new Vector();
       int hiddenOffset = viewport.getSelectionGroup().getStartRes();
@@ -1526,16 +1756,17 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   {
     for (int i = 0; i < seqs.length; i++)
     {
-      viewport.alignment.addSequence(seqs[i]);
+      viewport.getAlignment().addSequence(seqs[i]);
     }
 
     // !newAlignment
     addHistoryItem(new EditCommand("Add sequences", EditCommand.PASTE,
-            seqs, 0, viewport.alignment.getWidth(), viewport.alignment));
+            seqs, 0, viewport.getAlignment().getWidth(),
+            viewport.getAlignment()));
 
-    viewport.setEndSeq(viewport.alignment.getHeight());
-    viewport.alignment.getWidth();
-    viewport.firePropertyChange("alignment", null, viewport.alignment
+    viewport.setEndSeq(viewport.getAlignment().getHeight());
+    viewport.getAlignment().getWidth();
+    viewport.firePropertyChange("alignment", null, viewport.getAlignment()
             .getSequences());
 
   }
@@ -1564,7 +1795,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
 
     // If the cut affects all sequences, remove highlighted columns
-    if (sg.getSize() == viewport.alignment.getHeight())
+    if (sg.getSize() == viewport.getAlignment().getHeight())
     {
       viewport.getColumnSelection().removeElements(sg.getStartRes(),
               sg.getEndRes() + 1);
@@ -1581,10 +1812,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
      */
     addHistoryItem(new EditCommand("Cut Sequences", EditCommand.CUT, cut,
             sg.getStartRes(), sg.getEndRes() - sg.getStartRes() + 1,
-            viewport.alignment));
+            viewport.getAlignment()));
 
     viewport.setSelectionGroup(null);
-    viewport.alignment.deleteGroup(sg);
+    viewport.getAlignment().deleteGroup(sg);
 
     viewport.firePropertyChange("alignment", null, viewport.getAlignment()
             .getSequences());
@@ -1593,11 +1824,101 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       this.setVisible(false);
     }
+    viewport.sendSelection();
+  }
+
+  /**
+   * group consensus toggled
+   * 
+   */
+  protected void showGroupConsensus_actionPerformed()
+  {
+    viewport.setShowGroupConsensus(showGroupConsensus.getState());
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+
+  }
+
+  /**
+   * group conservation toggled.
+   */
+  protected void showGroupConservation_actionPerformed()
+  {
+    viewport.setShowGroupConservation(showGroupConservation.getState());
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+  }
+
+  /*
+   * (non-Javadoc)
+   * 
+   * @see
+   * jalview.jbgui.GAlignFrame#showConsensusHistogram_actionPerformed(java.awt
+   * .event.ActionEvent)
+   */
+  protected void showConsensusHistogram_actionPerformed()
+  {
+    viewport.setShowConsensusHistogram(showConsensusHistogram.getState());
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+  }
+
+  /*
+   * (non-Javadoc)
+   * 
+   * @see
+   * jalview.jbgui.GAlignFrame#showConsensusProfile_actionPerformed(java.awt
+   * .event.ActionEvent)
+   */
+  protected void showSequenceLogo_actionPerformed()
+  {
+    viewport.setShowSequenceLogo(showSequenceLogo.getState());
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+  }
+
+  protected void normSequenceLogo_actionPerformed()
+  {
+    showSequenceLogo.setState(true);
+    viewport.setShowSequenceLogo(true);
+    viewport.setNormaliseSequenceLogo(normSequenceLogo.getState());
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+  }
+
+  protected void applyAutoAnnotationSettings_actionPerformed()
+  {
+    alignPanel.updateAnnotation(applyAutoAnnotationSettings.getState());
+  }
+
+  protected void makeGrpsFromSelection_actionPerformed()
+  {
+    if (viewport.getSelectionGroup() != null)
+    {
+      SequenceGroup[] gps = jalview.analysis.Grouping.makeGroupsFrom(
+              viewport.getSequenceSelection(),
+              viewport.getAlignmentView(true).getSequenceStrings(
+                      viewport.getGapCharacter()), viewport.getAlignment()
+                      .getGroups());
+      viewport.getAlignment().deleteAllGroups();
+      viewport.sequenceColours = null;
+      viewport.setSelectionGroup(null);
+      // set view properties for each group
+      for (int g = 0; g < gps.length; g++)
+      {
+        // gps[g].setShowunconserved(viewport.getShowUnconserved());
+        gps[g].setshowSequenceLogo(viewport.isShowSequenceLogo());
+        viewport.getAlignment().addGroup(gps[g]);
+        Color col = new Color((int) (Math.random() * 255),
+                (int) (Math.random() * 255), (int) (Math.random() * 255));
+        col = col.brighter();
+        for (SequenceI sq : gps[g].getSequences(null))
+          viewport.setSequenceColour(sq, col);
+      }
+      PaintRefresher.Refresh(this, viewport.getSequenceSetId());
+      alignPanel.updateAnnotation();
+      alignPanel.paintAlignment(true);
+    }
   }
 
   protected void deleteGroups_actionPerformed()
   {
-    viewport.alignment.deleteAllGroups();
+    viewport.getAlignment().deleteAllGroups();
     viewport.sequenceColours = null;
     viewport.setSelectionGroup(null);
 
@@ -1611,10 +1932,11 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       sg.addSequence(viewport.getAlignment().getSequenceAt(i), false);
     }
-    sg.setEndRes(viewport.alignment.getWidth() - 1);
+    sg.setEndRes(viewport.getAlignment().getWidth() - 1);
     viewport.setSelectionGroup(sg);
     alignPanel.paintAlignment(true);
     PaintRefresher.Refresh(alignPanel, viewport.getSequenceSetId());
+    viewport.sendSelection();
   }
 
   public void deselectAllSequenceMenuItem_actionPerformed()
@@ -1631,6 +1953,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     alignPanel.seqPanel.seqCanvas.highlightSearchResults(null);
     alignPanel.paintAlignment(true);
     PaintRefresher.Refresh(alignPanel, viewport.getSequenceSetId());
+    viewport.sendSelection();
   }
 
   public void invertSequenceMenuItem_actionPerformed()
@@ -1642,6 +1965,15 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
 
     PaintRefresher.Refresh(alignPanel, viewport.getSequenceSetId());
+    viewport.sendSelection();
+  }
+
+  public void invertColSel_actionPerformed()
+  {
+    viewport.invertColumnSelection();
+    alignPanel.paintAlignment(true);
+    PaintRefresher.Refresh(alignPanel, viewport.getSequenceSetId());
+    viewport.sendSelection();
   }
 
   void trimAlignment(boolean trimLeft)
@@ -1664,11 +1996,11 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       if (viewport.getSelectionGroup() != null)
       {
         seqs = viewport.getSelectionGroup().getSequencesAsArray(
-                viewport.hiddenRepSequences);
+                viewport.getHiddenRepSequences());
       }
       else
       {
-        seqs = viewport.alignment.getSequencesArray();
+        seqs = viewport.getAlignment().getSequencesArray();
       }
 
       TrimRegionCommand trimRegion;
@@ -1676,32 +2008,28 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       {
         trimRegion = new TrimRegionCommand("Remove Left",
                 TrimRegionCommand.TRIM_LEFT, seqs, column,
-                viewport.alignment, viewport.colSel,
-                viewport.selectionGroup);
+                viewport.getAlignment(), viewport.getColumnSelection(),
+                viewport.getSelectionGroup());
         viewport.setStartRes(0);
       }
       else
       {
         trimRegion = new TrimRegionCommand("Remove Right",
                 TrimRegionCommand.TRIM_RIGHT, seqs, column,
-                viewport.alignment, viewport.colSel,
-                viewport.selectionGroup);
+                viewport.getAlignment(), viewport.getColumnSelection(),
+                viewport.getSelectionGroup());
       }
 
       statusBar.setText("Removed " + trimRegion.getSize() + " columns.");
 
       addHistoryItem(trimRegion);
 
-      Vector groups = viewport.alignment.getGroups();
-
-      for (int i = 0; i < groups.size(); i++)
+      for (SequenceGroup sg : viewport.getAlignment().getGroups())
       {
-        SequenceGroup sg = (SequenceGroup) groups.elementAt(i);
-
         if ((trimLeft && !sg.adjustForRemoveLeft(column))
                 || (!trimLeft && !sg.adjustForRemoveRight(column)))
         {
-          viewport.alignment.deleteGroup(sg);
+          viewport.getAlignment().deleteGroup(sg);
         }
       }
 
@@ -1712,23 +2040,24 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   public void removeGappedColumnMenuItem_actionPerformed()
   {
-    int start = 0, end = viewport.alignment.getWidth() - 1;
+    int start = 0, end = viewport.getAlignment().getWidth() - 1;
 
     SequenceI[] seqs;
     if (viewport.getSelectionGroup() != null)
     {
       seqs = viewport.getSelectionGroup().getSequencesAsArray(
-              viewport.hiddenRepSequences);
+              viewport.getHiddenRepSequences());
       start = viewport.getSelectionGroup().getStartRes();
       end = viewport.getSelectionGroup().getEndRes();
     }
     else
     {
-      seqs = viewport.alignment.getSequencesArray();
+      seqs = viewport.getAlignment().getSequencesArray();
     }
 
     RemoveGapColCommand removeGapCols = new RemoveGapColCommand(
-            "Remove Gapped Columns", seqs, start, end, viewport.alignment);
+            "Remove Gapped Columns", seqs, start, end,
+            viewport.getAlignment());
 
     addHistoryItem(removeGapCols);
 
@@ -1737,7 +2066,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
     // This is to maintain viewport position on first residue
     // of first sequence
-    SequenceI seq = viewport.alignment.getSequenceAt(0);
+    SequenceI seq = viewport.getAlignment().getSequenceAt(0);
     int startRes = seq.findPosition(viewport.startRes);
     // ShiftList shifts;
     // viewport.getAlignment().removeGaps(shifts=new ShiftList());
@@ -1752,28 +2081,28 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   public void removeAllGapsMenuItem_actionPerformed()
   {
-    int start = 0, end = viewport.alignment.getWidth() - 1;
+    int start = 0, end = viewport.getAlignment().getWidth() - 1;
 
     SequenceI[] seqs;
     if (viewport.getSelectionGroup() != null)
     {
       seqs = viewport.getSelectionGroup().getSequencesAsArray(
-              viewport.hiddenRepSequences);
+              viewport.getHiddenRepSequences());
       start = viewport.getSelectionGroup().getStartRes();
       end = viewport.getSelectionGroup().getEndRes();
     }
     else
     {
-      seqs = viewport.alignment.getSequencesArray();
+      seqs = viewport.getAlignment().getSequencesArray();
     }
 
     // This is to maintain viewport position on first residue
     // of first sequence
-    SequenceI seq = viewport.alignment.getSequenceAt(0);
+    SequenceI seq = viewport.getAlignment().getSequenceAt(0);
     int startRes = seq.findPosition(viewport.startRes);
 
     addHistoryItem(new RemoveGapsCommand("Remove Gaps", seqs, start, end,
-            viewport.alignment));
+            viewport.getAlignment()));
 
     viewport.setStartRes(seq.findIndex(startRes) - 1);
 
@@ -1796,35 +2125,34 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public AlignFrame newView(String viewtitle)
   {
     AlignmentI newal;
-    if (viewport.hasHiddenRows)
+    if (viewport.hasHiddenRows())
     {
       newal = new Alignment(viewport.getAlignment().getHiddenSequences()
               .getFullAlignment().getSequencesArray());
     }
     else
     {
-      newal = new Alignment(viewport.alignment.getSequencesArray());
+      newal = new Alignment(viewport.getAlignment().getSequencesArray());
     }
 
-    if (viewport.alignment.getAlignmentAnnotation() != null)
+    if (viewport.getAlignment().getAlignmentAnnotation() != null)
     {
-      for (int i = 0; i < viewport.alignment.getAlignmentAnnotation().length; i++)
+      for (int i = 0; i < viewport.getAlignment().getAlignmentAnnotation().length; i++)
       {
-        if (!viewport.alignment.getAlignmentAnnotation()[i].autoCalculated)
+        if (!viewport.getAlignment().getAlignmentAnnotation()[i].autoCalculated)
         {
-          newal
-                  .addAnnotation(viewport.alignment
-                          .getAlignmentAnnotation()[i]);
+          newal.addAnnotation(viewport.getAlignment()
+                  .getAlignmentAnnotation()[i]);
         }
       }
     }
 
     AlignFrame newaf = new AlignFrame(newal, viewport.applet, "", false);
 
-    newaf.viewport.sequenceSetID = alignPanel.av.getSequenceSetId();
+    newaf.viewport.setSequenceSetId(alignPanel.av.getSequenceSetId());
     PaintRefresher.Register(alignPanel, alignPanel.av.getSequenceSetId());
-    PaintRefresher.Register(newaf.alignPanel, newaf.alignPanel.av
-            .getSequenceSetId());
+    PaintRefresher.Register(newaf.alignPanel,
+            newaf.alignPanel.av.getSequenceSetId());
 
     PaintRefresher.Register(newaf.alignPanel.idPanel.idCanvas,
             newaf.alignPanel.av.getSequenceSetId());
@@ -1882,7 +2210,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
    * get sequence feature groups that are hidden or shown
    * 
    * @param visible
-   *                true is visible
+   *          true is visible
    * @return list
    */
   public String[] getFeatureGroupsOfState(boolean visible)
@@ -1900,9 +2228,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
    * Change the display state for the given feature groups
    * 
    * @param groups
-   *                list of group strings
+   *          list of group strings
    * @param state
-   *                visible or invisible
+   *          visible or invisible
    */
   public void setFeatureGroupState(String[] groups, boolean state)
   {
@@ -1966,11 +2294,16 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
             overview.getPreferredSize().height + 50);
 
     frame.pack();
+    final AlignmentPanel ap = alignPanel;
     frame.addWindowListener(new WindowAdapter()
     {
+      @Override
       public void windowClosing(WindowEvent e)
       {
-        alignPanel.setOverviewPanel(null);
+        if (ap != null)
+        {
+          ap.setOverviewPanel(null);
+        }
       };
     });
 
@@ -2001,13 +2334,13 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       if (viewport.getConservationSelected())
       {
 
-        Alignment al = (Alignment) viewport.alignment;
+        Alignment al = (Alignment) viewport.getAlignment();
         Conservation c = new Conservation("All",
-                ResidueProperties.propHash, 3, al.getSequences(), 0, al
-                        .getWidth() - 1);
+                ResidueProperties.propHash, 3, al.getSequences(), 0,
+                al.getWidth() - 1);
 
         c.calculate();
-        c.verdict(false, viewport.ConsPercGaps);
+        c.verdict(false, viewport.getConsPercGaps());
 
         cs.setConservation(c);
 
@@ -2020,81 +2353,19 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
         cs.setConservation(null);
       }
 
-      cs.setConsensus(viewport.hconsensus);
+      cs.setConsensus(viewport.getSequenceConsensusHash());
 
     }
     viewport.setGlobalColourScheme(cs);
 
-    if (viewport.getColourAppliesToAllGroups())
-    {
-      Vector groups = viewport.alignment.getGroups();
-      for (int i = 0; i < groups.size(); i++)
-      {
-        SequenceGroup sg = (SequenceGroup) groups.elementAt(i);
-
-        if (cs == null)
-        {
-          sg.cs = null;
-          continue;
-        }
-        if (cs instanceof ClustalxColourScheme)
-        {
-          sg.cs = new ClustalxColourScheme(sg
-                  .getSequences(viewport.hiddenRepSequences), sg.getWidth());
-        }
-        else
-        {
-          try
-          {
-            sg.cs = (ColourSchemeI) cs.getClass().newInstance();
-          } catch (Exception ex)
-          {
-            ex.printStackTrace();
-            sg.cs = cs;
-          }
-        }
-
-        if (viewport.getAbovePIDThreshold()
-                || cs instanceof PIDColourScheme
-                || cs instanceof Blosum62ColourScheme)
-        {
-          sg.cs.setThreshold(threshold, viewport.getIgnoreGapsConsensus());
-          sg.cs.setConsensus(AAFrequency.calculate(sg
-                  .getSequences(viewport.hiddenRepSequences), 0, sg
-                  .getWidth()));
-        }
-        else
-        {
-          sg.cs.setThreshold(0, viewport.getIgnoreGapsConsensus());
-        }
-
-        if (viewport.getConservationSelected())
-        {
-          Conservation c = new Conservation("Group",
-                  ResidueProperties.propHash, 3, sg
-                          .getSequences(viewport.hiddenRepSequences), 0,
-                  viewport.alignment.getWidth() - 1);
-          c.calculate();
-          c.verdict(false, viewport.ConsPercGaps);
-          sg.cs.setConservation(c);
-        }
-        else
-        {
-          sg.cs.setConservation(null);
-          sg.cs.setThreshold(0, viewport.getIgnoreGapsConsensus());
-        }
-
-      }
-    }
-
     if (alignPanel.getOverviewPanel() != null)
     {
       alignPanel.getOverviewPanel().updateOverviewImage();
     }
 
     jalview.structure.StructureSelectionManager
-            .getStructureSelectionManager().sequenceColoursChanged(
-                    alignPanel);
+            .getStructureSelectionManager(viewport.applet)
+            .sequenceColoursChanged(alignPanel);
 
     alignPanel.paintAlignment(true);
   }
@@ -2102,10 +2373,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   protected void modifyPID_actionPerformed()
   {
     if (viewport.getAbovePIDThreshold()
-            && viewport.globalColourScheme != null)
+            && viewport.getGlobalColourScheme() != null)
     {
-      SliderPanel.setPIDSliderSource(alignPanel, viewport
-              .getGlobalColourScheme(), "Background");
+      SliderPanel.setPIDSliderSource(alignPanel,
+              viewport.getGlobalColourScheme(), "Background");
       SliderPanel.showPIDSlider();
     }
   }
@@ -2113,10 +2384,10 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   protected void modifyConservation_actionPerformed()
   {
     if (viewport.getConservationSelected()
-            && viewport.globalColourScheme != null)
+            && viewport.getGlobalColourScheme() != null)
     {
       SliderPanel.setConservationSlider(alignPanel,
-              viewport.globalColourScheme, "Background");
+              viewport.getGlobalColourScheme(), "Background");
       SliderPanel.showConservationSlider();
     }
   }
@@ -2144,6 +2415,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
     modifyPID_actionPerformed();
   }
+
   public void sortPairwiseMenuItem_actionPerformed()
   {
     SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
@@ -2151,7 +2423,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
             .getAlignment().getSequenceAt(0), null);
 
     addHistoryItem(new OrderCommand("Pairwise Sort", oldOrder,
-            viewport.alignment));
+            viewport.getAlignment()));
     alignPanel.paintAlignment(true);
   }
 
@@ -2160,7 +2432,16 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
     AlignmentSorter.sortByID(viewport.getAlignment());
     addHistoryItem(new OrderCommand("ID Sort", oldOrder,
-            viewport.alignment));
+            viewport.getAlignment()));
+    alignPanel.paintAlignment(true);
+  }
+
+  public void sortLengthMenuItem_actionPerformed()
+  {
+    SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
+    AlignmentSorter.sortByLength(viewport.getAlignment());
+    addHistoryItem(new OrderCommand("Length Sort", oldOrder,
+            viewport.getAlignment()));
     alignPanel.paintAlignment(true);
   }
 
@@ -2169,7 +2450,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
     AlignmentSorter.sortByGroup(viewport.getAlignment());
     addHistoryItem(new OrderCommand("Group Sort", oldOrder,
-            viewport.alignment));
+            viewport.getAlignment()));
     alignPanel.paintAlignment(true);
 
   }
@@ -2194,7 +2475,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   public void PCAMenuItem_actionPerformed()
   {
     // are the sequences aligned?
-    if (!viewport.alignment.isAligned())
+    if (!viewport.getAlignment().isAligned(false))
     {
       SequenceI current;
       int Width = viewport.getAlignment().getWidth();
@@ -2251,7 +2532,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   void NewTreePanel(String type, String pwType, String title)
   {
     // are the sequences aligned?
-    if (!viewport.alignment.isAligned())
+    if (!viewport.getAlignment().isAligned(false))
     {
       SequenceI current;
       int Width = viewport.getAlignment().getWidth();
@@ -2271,10 +2552,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
     if ((viewport.getSelectionGroup() != null && viewport
             .getSelectionGroup().getSize() > 1)
-            || (viewport.getSelectionGroup() == null && viewport.alignment
-                    .getHeight() > 1))
+            || (viewport.getAlignment().getHeight() > 1))
     {
-      final TreePanel tp = new TreePanel(viewport, type, pwType);
+      final TreePanel tp = new TreePanel(alignPanel, type, pwType);
 
       addTreeMenuItem(tp, title);
 
@@ -2294,37 +2574,46 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   public void loadTree(jalview.io.NewickFile tree, String treeFile)
   {
-    TreePanel tp = new TreePanel(viewport, treeFile, "From File - ", tree);
+    TreePanel tp = new TreePanel(alignPanel, treeFile, "From File - ", tree);
     jalview.bin.JalviewLite.addFrame(tp, treeFile, 600, 500);
     addTreeMenuItem(tp, treeFile);
   }
+
   /**
    * sort the alignment using the given treePanel
-   * @param treePanel tree used to sort view
-   * @param title string used for undo event name
+   * 
+   * @param treePanel
+   *          tree used to sort view
+   * @param title
+   *          string used for undo event name
    */
   public void sortByTree(TreePanel treePanel, String title)
   {
     SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
-    AlignmentSorter.sortByTree(viewport.getAlignment(), treePanel
-            .getTree());
+    AlignmentSorter
+            .sortByTree(viewport.getAlignment(), treePanel.getTree());
     // addHistoryItem(new HistoryItem("Sort", viewport.alignment,
     // HistoryItem.SORT));
-    addHistoryItem(new OrderCommand("Order by "+title, oldOrder,
-            viewport.alignment));
+    addHistoryItem(new OrderCommand("Order by " + title, oldOrder,
+            viewport.getAlignment()));
     alignPanel.paintAlignment(true);
   }
+
   /**
-   * Do any automatic reordering of the alignment and add the necessary bits to the menu structure for the new tree
+   * Do any automatic reordering of the alignment and add the necessary bits to
+   * the menu structure for the new tree
+   * 
    * @param treePanel
    * @param title
    */
-  protected void addTreeMenuItem(final TreePanel treePanel, final String title)
+  protected void addTreeMenuItem(final TreePanel treePanel,
+          final String title)
   {
     final MenuItem item = new MenuItem(title);
     sortByTreeMenu.add(item);
     item.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent evt)
       {
         sortByTree(treePanel, title); // treePanel.getTitle());
@@ -2333,6 +2622,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
     treePanel.addWindowListener(new WindowAdapter()
     {
+      @Override
       public void windowOpened(WindowEvent e)
       {
         if (viewport.sortByTree)
@@ -2342,6 +2632,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
         super.windowOpened(e);
       }
 
+      @Override
       public void windowClosing(WindowEvent e)
       {
         sortByTreeMenu.remove(item);
@@ -2349,9 +2640,27 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     });
   }
 
+  public boolean sortBy(AlignmentOrder alorder, String undoname)
+  {
+    SequenceI[] oldOrder = viewport.getAlignment().getSequencesArray();
+    if (viewport.applet.debug)
+    {
+      System.err.println("Sorting " + alorder.getOrder().size()
+              + " in alignment '" + getTitle() + "'");
+    }
+    AlignmentSorter.sortBy(viewport.getAlignment(), alorder);
+    if (undoname != null)
+    {
+      addHistoryItem(new OrderCommand(undoname, oldOrder,
+              viewport.getAlignment()));
+    }
+    alignPanel.paintAlignment(true);
+    return true;
+  }
+
   protected void documentation_actionPerformed()
   {
-    showURL("http://www.jalview.org/help.html", "HELP");
+    alignPanel.av.applet.openJalviewHelpUrl();
   }
 
   protected void about_actionPerformed()
@@ -2361,11 +2670,15 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     {
       String version;
 
-      public AboutPanel(String version)
+      String builddate;
+
+      public AboutPanel(String version, String builddate)
       {
         this.version = version;
+        this.builddate = builddate;
       }
 
+      @Override
       public void paint(Graphics g)
       {
         g.setColor(Color.white);
@@ -2379,52 +2692,34 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
         // lite and application
         g.setFont(new Font("Helvetica", Font.BOLD, 14));
         g.drawString("JalviewLite - Release " + version, x, y += fh);
+        g.setFont(new Font("Helvetica", Font.BOLD, 12));
+        g.drawString("Build date: " + builddate, x, y += fh);
         g.setFont(new Font("Helvetica", Font.PLAIN, 12));
-        g
-                .drawString(
-                        "Authors:  Andrew Waterhouse, Jim Procter, Michele Clamp, James Cuff, Steve Searle,",
-                        x, y += fh * 1.5);
-        g.drawString("David Martin & Geoff Barton.", x + 50, y += fh);
-        g
-                .drawString(
-                        "Development managed by The Barton Group, University of Dundee, Scotland, UK.",
-                        x, y += fh);
-        g
-                .drawString(
-                        "For help, see the FAQ at www.jalview.org and/or join the jalview-discuss@jalview.org mailing list",
-                        x, y += fh);
+        g.drawString(
+                "Authors:  Jim Procter, Andrew Waterhouse, Jan Engelhardt, Lauren Lui,",
+                x, y += fh * 1.5);
+        g.drawString("Michele Clamp, James Cuff, Steve Searle, David Martin & Geoff Barton.", x + 50, y += fh+8);
+        g.drawString(
+                "Development managed by The Barton Group, University of Dundee, Scotland, UK.",
+                x, y += fh);
+        g.drawString(
+                "For help, see the FAQ at www.jalview.org and/or join the jalview-discuss@jalview.org mailing list",
+                x, y += fh);
         g.drawString("If  you use Jalview, please cite:", x, y += fh + 8);
         g.drawString(
-                        "Waterhouse, A.M., Procter, J.B., Martin, D.M.A, Clamp, M. and Barton, G. J. (2009)",x,y+=fh);
-        g.drawString("Jalview Version 2 - a multiple sequence alignment editor and analysis workbench",x,y+=fh);
-        g.drawString("Bioinformatics doi: 10.1093/bioinformatics/btp033",x,y+=fh);
-      }
-    }
-
-    String version = "test";
-    java.net.URL url = getClass().getResource("/.build_properties");
-    if (url != null)
-    {
-      try
-      {
-        BufferedReader reader = new BufferedReader(new InputStreamReader(
-                url.openStream()));
-        String line;
-        while ((line = reader.readLine()) != null)
-        {
-          if (line.indexOf("VERSION") > -1)
-          {
-            version = line.substring(line.indexOf("=") + 1);
-          }
-        }
-      } catch (Exception ex)
-      {
-        ex.printStackTrace();
+                "Waterhouse, A.M., Procter, J.B., Martin, D.M.A, Clamp, M. and Barton, G. J. (2009)",
+                x, y += fh);
+        g.drawString(
+                "Jalview Version 2 - a multiple sequence alignment editor and analysis workbench",
+                x, y += fh);
+        g.drawString("Bioinformatics doi: 10.1093/bioinformatics/btp033",
+                x, y += fh);
       }
     }
 
     Frame frame = new Frame();
-    frame.add(new AboutPanel(version));
+    frame.add(new AboutPanel(JalviewLite.getVersion(), JalviewLite
+            .getBuildDate()));
     jalview.bin.JalviewLite.addFrame(frame, "Jalview", 580, 220);
 
   }
@@ -2437,28 +2732,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     }
     else
     {
-      try
-      {
-        if (url.indexOf(":")==-1)
-        {
-          // TODO: verify (Bas Vroling bug) prepend codebase or server URL to form valid URL
-          if (url.indexOf("/")==0)
-          {
-            String codebase = viewport.applet.getCodeBase().toString();
-            url = codebase.substring(0,codebase.length()-viewport.applet.getCodeBase().getFile().length())+url;
-          } else {
-            url = viewport.applet.getCodeBase()+url;
-          }
-          System.out.println("Show url (prepended codebase): " + url);
-        } else {
-          System.out.println("Show url: " + url);
-        }
-        viewport.applet.getAppletContext().showDocument(
-                new java.net.URL(url), target);
-      } catch (Exception ex)
-      {
-        ex.printStackTrace();
-      }
+      viewport.applet.showURL(url, target);
     }
   }
 
@@ -2511,6 +2785,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   MenuItem sortIDMenuItem = new MenuItem();
 
+  MenuItem sortLengthMenuItem = new MenuItem();
+
   MenuItem sortGroupMenuItem = new MenuItem();
 
   MenuItem removeRedundancyMenuItem = new MenuItem();
@@ -2545,12 +2821,18 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   MenuItem buriedColour = new MenuItem();
 
+  MenuItem purinePyrimidineColour = new MenuItem();
+
+  MenuItem RNAHelixColour = new MenuItem();
+
   MenuItem userDefinedColour = new MenuItem();
 
   MenuItem PIDColour = new MenuItem();
 
   MenuItem BLOSUM62Colour = new MenuItem();
 
+  MenuItem tcoffeeColour = new MenuItem();
+
   MenuItem njTreeBlosumMenuItem = new MenuItem();
 
   MenuItem avDistanceTreeBlosumMenuItem = new MenuItem();
@@ -2585,6 +2867,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   MenuItem deleteGroups = new MenuItem();
 
+  MenuItem grpsFromSelection = new MenuItem();
+
   MenuItem delete = new MenuItem();
 
   MenuItem copy = new MenuItem();
@@ -2614,6 +2898,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
   CheckboxMenuItem autoCalculate = new CheckboxMenuItem(
           "Autocalculate Consensus", true);
 
+  CheckboxMenuItem sortByTree = new CheckboxMenuItem(
+          "Sort Alignment With New Tree", true);
+
   Menu sortByTreeMenu = new Menu();
 
   Menu sort = new Menu();
@@ -2634,6 +2921,20 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   CheckboxMenuItem followMouseOverFlag = new CheckboxMenuItem();
 
+  Menu autoAnnMenu = new Menu();
+
+  CheckboxMenuItem showSequenceLogo = new CheckboxMenuItem();
+
+  CheckboxMenuItem applyAutoAnnotationSettings = new CheckboxMenuItem();
+
+  CheckboxMenuItem showConsensusHistogram = new CheckboxMenuItem();
+
+  CheckboxMenuItem showGroupConsensus = new CheckboxMenuItem();
+
+  CheckboxMenuItem showGroupConservation = new CheckboxMenuItem();
+
+  CheckboxMenuItem normSequenceLogo = new CheckboxMenuItem();
+
   private void jbInit() throws Exception
   {
 
@@ -2650,6 +2951,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
       item.addActionListener(new java.awt.event.ActionListener()
       {
+        @Override
         public void actionPerformed(ActionEvent e)
         {
           outputText_actionPerformed(e);
@@ -2686,6 +2988,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     sortPairwiseMenuItem.addActionListener(this);
     sortIDMenuItem.setLabel("by ID");
     sortIDMenuItem.addActionListener(this);
+    sortLengthMenuItem.setLabel("by Length");
+    sortLengthMenuItem.addActionListener(this);
     sortGroupMenuItem.setLabel("by Group");
     sortGroupMenuItem.addActionListener(this);
     removeRedundancyMenuItem.setLabel("Remove Redundancy...");
@@ -2720,12 +3024,20 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     turnColour.addActionListener(this);
     buriedColour.setLabel("Buried Index");
     buriedColour.addActionListener(this);
+    purinePyrimidineColour.setLabel("Purine/Pyrimidine");
+    purinePyrimidineColour.addActionListener(this);
+    RNAHelixColour.setLabel("by RNA Helices");
+    RNAHelixColour.addActionListener(this);
     userDefinedColour.setLabel("User Defined...");
     userDefinedColour.addActionListener(this);
     PIDColour.setLabel("Percentage Identity");
     PIDColour.addActionListener(this);
     BLOSUM62Colour.setLabel("BLOSUM62 Score");
     BLOSUM62Colour.addActionListener(this);
+    tcoffeeColour.setLabel("T-Coffee Scores");
+    tcoffeeColour.setEnabled(false); // it will enabled only if a score file is
+                                     // provided
+    tcoffeeColour.addActionListener(this);
     avDistanceTreeBlosumMenuItem
             .setLabel("Average Distance Using BLOSUM62");
     avDistanceTreeBlosumMenuItem.addActionListener(this);
@@ -2735,7 +3047,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     annotationPanelMenuItem.addItemListener(this);
     colourTextMenuItem.setLabel("Colour Text");
     colourTextMenuItem.addItemListener(this);
-    displayNonconservedMenuItem.setLabel("Show non-conserved");
+    displayNonconservedMenuItem.setLabel("Show nonconserved");
     displayNonconservedMenuItem.addItemListener(this);
     alProperties.addActionListener(this);
     overviewMenuItem.setLabel("Overview Window");
@@ -2763,6 +3075,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     nucleotideColour.addActionListener(this);
     deleteGroups.setLabel("Undefine Groups");
     deleteGroups.addActionListener(this);
+    grpsFromSelection.setLabel("Make Groups for selection");
+    grpsFromSelection.addActionListener(this);
     copy.setLabel("Copy");
     copy.addActionListener(this);
     cut.setLabel("Cut");
@@ -2799,6 +3113,7 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     sort.setLabel("Sort");
     calculate.setLabel("Calculate Tree");
     autoCalculate.addItemListener(this);
+    sortByTree.addItemListener(this);
     inputText.setLabel("Input from textbox");
     inputText.addActionListener(this);
     centreColumnLabelFlag.setLabel("Centre column labels");
@@ -2829,11 +3144,33 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     menu2.setLabel("Hide");
     hideColumns.setLabel("Selected Columns");
     hideSequences.setLabel("Selected Sequences");
+    hideAllButSelection.setLabel("All but Selected Region (Shift+Ctrl+H)");
+    hideAllSelection.setLabel("Selected Region");
+    showAllHidden.setLabel("All Sequences and Columns");
+    showGroupConsensus.setLabel("Group Consensus");
+    showGroupConservation.setLabel("Group Conservation");
+    showConsensusHistogram.setLabel("Show Consensus Histogram");
+    showSequenceLogo.setLabel("Show Consensus Logo");
+    normSequenceLogo.setLabel("Normalise Consensus Logo");
+    applyAutoAnnotationSettings.setLabel("Apply to all groups");
+    applyAutoAnnotationSettings.setState(true);
+    autoAnnMenu.setLabel("Autocalculated Annotation");
+
     invertColSel.addActionListener(this);
     showColumns.addActionListener(this);
     showSeqs.addActionListener(this);
     hideColumns.addActionListener(this);
     hideSequences.addActionListener(this);
+    hideAllButSelection.addActionListener(this);
+    hideAllSelection.addActionListener(this);
+    showAllHidden.addActionListener(this);
+    showGroupConsensus.addItemListener(this);
+    showGroupConservation.addItemListener(this);
+    showConsensusHistogram.addItemListener(this);
+    showSequenceLogo.addItemListener(this);
+    normSequenceLogo.addItemListener(this);
+
+    applyAutoAnnotationSettings.addItemListener(this);
     formatMenu.setLabel("Format");
     selectMenu.setLabel("Select");
     newView.setLabel("New View");
@@ -2883,6 +3220,14 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     viewMenu.addSeparator();
     viewMenu.add(followMouseOverFlag);
     viewMenu.add(annotationPanelMenuItem);
+    autoAnnMenu.add(applyAutoAnnotationSettings);
+    autoAnnMenu.add(showConsensusHistogram);
+    autoAnnMenu.add(showSequenceLogo);
+    autoAnnMenu.add(normSequenceLogo);
+    autoAnnMenu.addSeparator();
+    autoAnnMenu.add(showGroupConservation);
+    autoAnnMenu.add(showGroupConsensus);
+    viewMenu.add(autoAnnMenu);
     viewMenu.addSeparator();
     viewMenu.add(sequenceFeatures);
     viewMenu.add(featureSettings);
@@ -2904,6 +3249,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     colourMenu.add(turnColour);
     colourMenu.add(buriedColour);
     colourMenu.add(nucleotideColour);
+    colourMenu.add(purinePyrimidineColour);
+    colourMenu.add(tcoffeeColour);
     colourMenu.add(userDefinedColour);
     colourMenu.addSeparator();
     colourMenu.add(conservationMenuItem);
@@ -2911,16 +3258,19 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     colourMenu.add(abovePIDThreshold);
     colourMenu.add(modifyPID);
     colourMenu.add(annotationColour);
+    colourMenu.add(RNAHelixColour);
     calculateMenu.add(sort);
     calculateMenu.add(calculate);
     calculateMenu.addSeparator();
     calculateMenu.add(pairwiseAlignmentMenuItem);
     calculateMenu.add(PCAMenuItem);
     calculateMenu.add(autoCalculate);
+    calculateMenu.add(sortByTree);
     this.add(statusBar, BorderLayout.SOUTH);
     pasteMenu.add(pasteNew);
     pasteMenu.add(pasteThis);
     sort.add(sortIDMenuItem);
+    sort.add(sortLengthMenuItem);
     sort.add(sortByTreeMenu);
     sort.add(sortGroupMenuItem);
     sort.add(sortPairwiseMenuItem);
@@ -2932,8 +3282,11 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     helpMenu.add(about);
     menu1.add(showColumns);
     menu1.add(showSeqs);
+    menu1.add(showAllHidden);
     menu2.add(hideColumns);
     menu2.add(hideSequences);
+    menu2.add(hideAllSelection);
+    menu2.add(hideAllButSelection);
     formatMenu.add(font);
     formatMenu.add(seqLimits);
     formatMenu.add(wrapMenuItem);
@@ -2952,7 +3305,9 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
     selectMenu.add(deselectAllSequenceMenuItem);
     selectMenu.add(invertSequenceMenuItem);
     selectMenu.add(invertColSel);
+    selectMenu.add(grpsFromSelection);
     selectMenu.add(deleteGroups);
+
   }
 
   MenuItem featureSettings = new MenuItem();
@@ -2975,6 +3330,12 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
 
   MenuItem hideSequences = new MenuItem();
 
+  MenuItem hideAllButSelection = new MenuItem();
+
+  MenuItem hideAllSelection = new MenuItem();
+
+  MenuItem showAllHidden = new MenuItem();
+
   Menu formatMenu = new Menu();
 
   Menu selectMenu = new Menu();
@@ -2987,8 +3348,8 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
    * platform independent manner to avoid OSX/Mac menu appendage daftness.
    * 
    * @param reallyEmbedded
-   *                true to attach the view to the applet area on the page
-   *                rather than in a new window
+   *          true to attach the view to the applet area on the page rather than
+   *          in a new window
    */
   public void createAlignFrameWindow(boolean reallyEmbedded, String title)
   {
@@ -3006,10 +3367,29 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
       viewport.applet.setLayout(new BorderLayout());
       viewport.applet.add(embeddedMenu, BorderLayout.NORTH);
       viewport.applet.add(statusBar, BorderLayout.SOUTH);
-      alignPanel.setSize(viewport.applet.getSize().width, viewport.applet
-              .getSize().height
-              - embeddedMenu.HEIGHT - statusBar.HEIGHT);
+      alignPanel.setSize(viewport.applet.getSize().width,
+              viewport.applet.getSize().height - embeddedMenu.HEIGHT
+                      - statusBar.HEIGHT);
       viewport.applet.add(alignPanel, BorderLayout.CENTER);
+      final AlignFrame me = this;
+      viewport.applet.addFocusListener(new FocusListener()
+      {
+
+        @Override
+        public void focusLost(FocusEvent e)
+        {
+          if (me.viewport.applet.currentAlignFrame == me)
+          {
+            me.viewport.applet.currentAlignFrame = null;
+          }
+        }
+
+        @Override
+        public void focusGained(FocusEvent e)
+        {
+          me.viewport.applet.currentAlignFrame = me;
+        }
+      });
       viewport.applet.validate();
     }
     else
@@ -3030,4 +3410,345 @@ public class AlignFrame extends EmbmenuFrame implements ActionListener,
               DEFAULT_HEIGHT);
     }
   }
+
+  /**
+   * create a new binding between structures in an existing jmol viewer instance
+   * and an alignpanel with sequences that have existing PDBFile entries. Note,
+   * this does not open a new Jmol window, or modify the display of the
+   * structures in the original jmol window. Note This method doesn't work
+   * without an additional javascript library to exchange messages between the
+   * distinct applets. See http://issues.jalview.org/browse/JAL-621
+   * 
+   * @param viewer
+   *          JmolViewer instance
+   * @param sequenceIds
+   *          - sequence Ids to search for associations
+   */
+  public SequenceStructureBinding addStructureViewInstance(
+          Object jmolviewer, String[] sequenceIds)
+  {
+    org.jmol.api.JmolViewer viewer = null;
+    try
+    {
+      viewer = (org.jmol.api.JmolViewer) jmolviewer;
+    } catch (ClassCastException ex)
+    {
+      System.err.println("Unsupported viewer object :"
+              + jmolviewer.getClass());
+    }
+    if (viewer == null)
+    {
+      System.err.println("Can't use this object as a structure viewer:"
+              + jmolviewer.getClass());
+      return null;
+    }
+    SequenceI[] seqs = null;
+    if (sequenceIds == null || sequenceIds.length == 0)
+    {
+      seqs = viewport.getAlignment().getSequencesArray();
+    }
+    else
+    {
+      Vector sqi = new Vector();
+      AlignmentI al = viewport.getAlignment();
+      for (int sid = 0; sid < sequenceIds.length; sid++)
+      {
+        SequenceI sq = al.findName(sequenceIds[sid]);
+        if (sq != null)
+        {
+          sqi.addElement(sq);
+        }
+      }
+      if (sqi.size() > 0)
+      {
+        seqs = new SequenceI[sqi.size()];
+        for (int sid = 0, sSize = sqi.size(); sid < sSize; sid++)
+        {
+          seqs[sid] = (SequenceI) sqi.elementAt(sid);
+        }
+      }
+      else
+      {
+        return null;
+      }
+    }
+    ExtJmol jmv = null;
+    // TODO: search for a jmv that involves viewer
+    if (jmv == null)
+    { // create a new viewer/jalview binding.
+      jmv = new ExtJmol(viewer, alignPanel, new SequenceI[][]
+      { seqs });
+    }
+    return jmv;
+
+  }
+
+  /**
+   * bind a pdb file to a sequence in the current view
+   * 
+   * @param sequenceId
+   *          - sequenceId within the dataset.
+   * @param pdbEntryString
+   *          - the short name for the PDB file
+   * @param pdbFile
+   *          - pdb file - either a URL or a valid PDB file.
+   * @return true if binding was as success TODO: consider making an exception
+   *         structure for indicating when PDB parsing or sequenceId location
+   *         fails.
+   */
+  public boolean addPdbFile(String sequenceId, String pdbEntryString,
+          String pdbFile)
+  {
+    SequenceI toaddpdb = viewport.getAlignment().findName(sequenceId);
+    boolean needtoadd = false;
+    if (toaddpdb != null)
+    {
+      Vector pdbe = toaddpdb.getPDBId();
+      PDBEntry pdbentry = null;
+      if (pdbe != null && pdbe.size() > 0)
+      {
+        for (int pe = 0, peSize = pdbe.size(); pe < peSize; pe++)
+        {
+          pdbentry = (PDBEntry) pdbe.elementAt(pe);
+          if (!pdbentry.getId().equals(pdbEntryString)
+                  && !pdbentry.getFile().equals(pdbFile))
+          {
+            pdbentry = null;
+          }
+          else
+          {
+            continue;
+          }
+        }
+      }
+      if (pdbentry == null)
+      {
+        pdbentry = new PDBEntry();
+        pdbentry.setId(pdbEntryString);
+        pdbentry.setFile(pdbFile);
+        needtoadd = true; // add this new entry to sequence.
+      }
+      // resolve data source
+      // TODO: this code should be a refactored to an io package
+      String protocol = AppletFormatAdapter.resolveProtocol(pdbFile, "PDB");
+      if (protocol == null)
+      {
+        return false;
+      }
+      if (needtoadd)
+      {
+        // make a note of the access mode and add
+        if (pdbentry.getProperty() == null)
+        {
+          pdbentry.setProperty(new Hashtable());
+        }
+        pdbentry.getProperty().put("protocol", protocol);
+        toaddpdb.addPDBId(pdbentry);
+      }
+    }
+    return true;
+  }
+
+  private Object[] cleanSeqChainArrays(SequenceI[] seqs, String[] chains)
+  {
+    if (seqs != null)
+    {
+      Vector sequences = new Vector();
+      for (int i = 0; i < seqs.length; i++)
+      {
+        if (seqs[i] != null)
+        {
+          sequences.addElement(new Object[]
+          { seqs[i], (chains != null) ? chains[i] : null });
+        }
+      }
+      seqs = new SequenceI[sequences.size()];
+      chains = new String[sequences.size()];
+      for (int i = 0, isize = sequences.size(); i < isize; i++)
+      {
+        Object[] oj = (Object[]) sequences.elementAt(i);
+
+        seqs[i] = (SequenceI) oj[0];
+        chains[i] = (String) oj[1];
+      }
+    }
+    return new Object[]
+    { seqs, chains };
+
+  }
+
+  public void newStructureView(JalviewLite applet, PDBEntry pdb,
+          SequenceI[] seqs, String[] chains, String protocol)
+  {
+    // Scrub any null sequences from the array
+    Object[] sqch = cleanSeqChainArrays(seqs, chains);
+    seqs = (SequenceI[]) sqch[0];
+    chains = (String[]) sqch[1];
+    if (seqs == null || seqs.length == 0)
+    {
+      System.err
+              .println("JalviewLite.AlignFrame:newStructureView: No sequence to bind structure to.");
+    }
+    if (protocol == null || protocol.trim().length() == 0
+            || protocol.equals("null"))
+    {
+      protocol = (String) pdb.getProperty().get("protocol");
+      if (protocol == null)
+      {
+        System.err.println("Couldn't work out protocol to open structure: "
+                + pdb.getId());
+        return;
+      }
+    }
+    if (applet.useXtrnalSviewer)
+    {
+      // register the association(s) and quit, don't create any windows.
+      if (StructureSelectionManager.getStructureSelectionManager(applet)
+              .setMapping(seqs, chains, pdb.getFile(), protocol) == null)
+      {
+        System.err.println("Failed to map " + pdb.getFile() + " ("
+                + protocol + ") to any sequences");
+      }
+      return;
+    }
+    if (applet.isAlignPdbStructures() && applet.jmolAvailable)
+    {
+      // can only do alignments with Jmol
+      // find the last jmol window assigned to this alignment
+      jalview.appletgui.AppletJmol ajm = null, tajm;
+      Vector jmols = applet
+              .getAppletWindow(jalview.appletgui.AppletJmol.class);
+      for (int i = 0, iSize = jmols.size(); i < iSize; i++)
+      {
+        tajm = (jalview.appletgui.AppletJmol) jmols.elementAt(i);
+        if (tajm.ap.alignFrame == this)
+        {
+          ajm = tajm;
+          break;
+        }
+      }
+      if (ajm != null)
+      {
+        System.err
+                .println("Incremental adding and aligning structure to existing Jmol view not yet implemented.");
+        // try and add the pdb structure
+        // ajm.addS
+        ajm = null;
+      }
+    }
+    // otherwise, create a new window
+    if (applet.jmolAvailable)
+    {
+      new jalview.appletgui.AppletJmol(pdb, seqs, chains, alignPanel,
+              protocol);
+      applet.lastFrameX += 40;
+      applet.lastFrameY += 40;
+    }
+    else
+    {
+      new MCview.AppletPDBViewer(pdb, seqs, chains, alignPanel, protocol);
+    }
+
+  }
+
+  public void alignedStructureView(JalviewLite applet, PDBEntry[] pdb,
+          SequenceI[][] seqs, String[][] chains, String[] protocols)
+  {
+    // TODO Auto-generated method stub
+    System.err.println("Aligned Structure View: Not yet implemented.");
+  }
+
+  /**
+   * modify the current selection, providing the user has not made a selection
+   * already.
+   * 
+   * @param sel
+   *          - sequences from this alignment
+   * @param csel
+   *          - columns to be selected on the alignment
+   */
+  public void select(SequenceGroup sel, ColumnSelection csel)
+  {
+    alignPanel.seqPanel.selection(sel, csel, null);
+  }
+
+  public void scrollTo(int row, int column)
+  {
+    alignPanel.seqPanel.scrollTo(row, column);
+  }
+
+  public void scrollToRow(int row)
+  {
+    alignPanel.seqPanel.scrollToRow(row);
+  }
+
+  public void scrollToColumn(int column)
+  {
+    alignPanel.seqPanel.scrollToColumn(column);
+  }
+
+  /**
+   * @return the alignments unique ID.
+   */
+  public String getSequenceSetId()
+  {
+    return viewport.getSequenceSetId();
+  }
+
+  /**
+   * Load the (T-Coffee) score file from the specified url
+   * 
+   * @param source
+   *          File/URL/T-COFFEE score file contents
+   * @throws IOException
+   * @return true if alignment was annotated with data from source
+   */
+  public boolean loadScoreFile(String source) throws IOException
+  {
+
+    TCoffeeScoreFile file = new TCoffeeScoreFile(source,
+            AppletFormatAdapter.checkProtocol(source));
+    if (!file.isValid())
+    {
+      // TODO: raise dialog for gui
+      System.err.println("Problems parsing T-Coffee scores: "
+              + file.getWarningMessage());
+      System.err.println("Origin was:\n" + source);
+      return false;
+    }
+
+    /*
+     * check that the score matrix matches the alignment dimensions
+     */
+    AlignmentI aln;
+    if ((aln = viewport.getAlignment()) != null
+            && (aln.getHeight() != file.getHeight() || aln.getWidth() != file
+                    .getWidth()))
+    {
+      // TODO: raise a dialog box here rather than bomb out.
+      System.err
+              .println("The scores matrix does not match the alignment dimensions");
+
+    }
+
+    // TODO add parameter to indicate if matching should be done
+    if (file.annotateAlignment(alignPanel.getAlignment(), false))
+    {
+      alignPanel.fontChanged();
+      tcoffeeColour.setEnabled(true);
+      // switch to this color
+      changeColour(new TCoffeeColourScheme(alignPanel.getAlignment()));
+      return true;
+    }
+    else
+    {
+      System.err.println("Problems resolving T-Coffee scores:");
+      if (file.getWarningMessage() != null)
+      {
+        System.err.println(file.getWarningMessage());
+      }
+    }
+    return false;
+  }
+
 }