import static org.testng.Assert.assertEquals;
import static org.testng.Assert.assertTrue;
-import static org.testng.Assert.assertNull;
+import static org.testng.AssertJUnit.assertNotNull;
+import static org.testng.AssertJUnit.assertNull;
+import static org.testng.AssertJUnit.assertSame;
+import static org.testng.AssertJUnit.fail;
import java.io.File;
import java.io.IOException;
import java.net.MalformedURLException;
+import java.util.Arrays;
import java.util.List;
import java.util.Set;
+import org.testng.annotations.BeforeClass;
import org.testng.annotations.Test;
+import jalview.bin.Cache;
import jalview.datamodel.DBRefEntry;
+import jalview.datamodel.Mapping;
import jalview.datamodel.SequenceFeature;
import jalview.datamodel.SequenceI;
import jalview.datamodel.features.SequenceFeatures;
+import jalview.util.MapList;
public class EmblFlatFileTest
{
+ @BeforeClass(alwaysRun = true)
+ public void setUp()
+ {
+ Cache.initLogger();
+ }
+
/**
* A fairly tough test, using J03321 (circular DNA), which has 8 CDS features,
* one of them reverse strand
public void testParse() throws MalformedURLException, IOException
{
File dataFile = new File("test/jalview/io/J03321.embl.txt");
- FileParse fp = new FileParse(dataFile, DataSourceType.FILE);
+ FileParse fp = new FileParse(dataFile.getAbsolutePath(), DataSourceType.FILE);
EmblFlatFile parser = new EmblFlatFile(fp, "EmblTest");
parser.parse();
List<SequenceI> seqs = parser.getSeqs();
SequenceI seq = seqs.get(0);
assertEquals(seq.getName(), "EmblTest|J03321");
assertEquals(seq.getLength(), 7502);
+ assertEquals(seq.getDescription(),
+ "Chlamydia trachomatis plasmid pCHL1, complete sequence");
/*
* should be 9 CDS features (one is a 'join' of two exons)
Set<String> featureTypes = seq.getFeatures().getFeatureTypes();
assertEquals(featureTypes.size(), 1);
assertTrue(featureTypes.contains("CDS"));
-
+
/*
- * inspect some features (sort them for convenience of test assertions)
+ * inspect some features (sorted just for convenience of test assertions)
*/
List<SequenceFeature> features = seq.getFeatures()
.getAllFeatures("CDS");
- SequenceFeatures.sortFeatures(features, true);
+ SequenceFeatures.sortFeatures(features, true);
assertEquals(features.size(), 9);
-
+
SequenceFeature sf = features.get(0);
assertEquals(sf.getBegin(), 1);
assertEquals(sf.getEnd(), 437);
assertEquals(sf.getPhase(), "0");
assertEquals(sf.getStrand(), 1);
assertEquals(sf.getValue("note"), "pGP7-D");
- // second exon of circular DNA!
+ // this is the second exon of circular CDS!
assertEquals(sf.getValue("exon number"), 2);
assertEquals(sf.getValue("product"), "hypothetical protein");
assertEquals(sf.getValue("transl_table"), "11");
-
+
sf = features.get(1);
assertEquals(sf.getBegin(), 488);
assertEquals(sf.getEnd(), 1480);
assertEquals(sf.getValue("note"), "pGP8-D");
assertEquals(sf.getValue("exon number"), 1);
assertEquals(sf.getValue("product"), "hypothetical protein");
-
+
sf = features.get(7);
assertEquals(sf.getBegin(), 6045);
assertEquals(sf.getEnd(), 6788);
assertEquals(sf.getValue("note"), "pGP6-D (gtg start codon)");
assertEquals(sf.getValue("exon number"), 1);
assertEquals(sf.getValue("product"), "hypothetical protein");
-
+
/*
- * CDS at 7022-7502 is the first exon of the circular DNA CDS
+ * CDS at 7022-7502 is the first exon of the circular CDS
*/
sf = features.get(8);
assertEquals(sf.getBegin(), 7022);
assertEquals(sf.getValue("product"), "hypothetical protein");
/*
- * there are 4 'direct' (DR) dbrefs, and numerous CDS /db_xref entries,
- * some of them (e.g. INTERPRO) duplicates; sample a few here
- * Note DBRefEntry constructor capitalises source
+ * Verify DBRefs, whether declared in the file or added by Jalview.
+ * There are 4 'direct' (DR) dbrefs, and numerous CDS /db_xref entries
+ * (some e.g. INTERPRO are duplicates). Jalview adds a dbref to 'self'.
+ * Sample a few here. Note DBRefEntry constructor capitalises source.
*/
- List<DBRefEntry> dbrefs = seq.getDBRefs();
- assertEquals(dbrefs.size(), 31);
+ List<DBRefEntry> dbrefs = Arrays.asList(seq.getDBRefs());
+
+ assertEquals(dbrefs.size(), 32);
+ // xref to 'self':
+ DBRefEntry selfRef = new DBRefEntry("EMBLTEST", "1", "J03321");
+ int[] range = new int[] { 1, seq.getLength() };
+ selfRef.setMap(new Mapping(null, range, range, 1, 1));
+ assertTrue(dbrefs.contains(selfRef));
+
// 1st DR line; note trailing period is removed
assertTrue(dbrefs.contains(new DBRefEntry("MD5", "0",
"d4c4942a634e3df4995fd5ac75c26a61")));
// the 4th DR line:
assertTrue(
- dbrefs.contains(new DBRefEntry("EuropePMC", "0", "PMC87941")));
- // from the first CDS feature; note canonicalisation to "UNIPROT"
+ dbrefs.contains(new DBRefEntry("EUROPEPMC", "0", "PMC87941")));
+ // from the first CDS feature
assertTrue(dbrefs.contains(new DBRefEntry("GOA", "0", "P0CE19")));
- assertTrue(dbrefs.contains(new DBRefEntry("UNIPROT", "0", "P0CE19")));
// from the last CDS feature
- assertTrue(dbrefs.contains(new DBRefEntry("INTERPRO", "0", "IPR005350")));
+ assertTrue(
+ dbrefs.contains(new DBRefEntry("INTERPRO", "0", "IPR005350")));
+
+ /*
+ * verify mappings to, and sequences for, UNIPROT proteins
+ */
+ int uniprotCount = 0;
+ List<int[]> ranges;
+ for (DBRefEntry dbref : dbrefs)
+ {
+ if ("UNIPROT".equals(dbref.getSource()))
+ {
+ uniprotCount++;
+ Mapping mapping = dbref.getMap();
+ assertNotNull(mapping);
+ MapList map = mapping.getMap();
+ String mappedToName = mapping.getTo().getName();
+ if ("UNIPROT|P0CE16".equals(mappedToName))
+ {
+ assertEquals((ranges = map.getFromRanges()).size(), 1);
+ assertEquals(ranges.get(0)[0], 1579);
+ assertEquals(ranges.get(0)[1], 2931);
+ assertEquals((ranges = map.getToRanges()).size(), 1);
+ assertEquals(ranges.get(0)[0], 1);
+ assertEquals(ranges.get(0)[1], 451);
+ // CDS /product carries over as protein product description
+ assertEquals(mapping.getTo().getDescription(),
+ "hypothetical protein");
+ }
+ else if ("UNIPROT|P0CE17".equals(mappedToName))
+ {
+ assertEquals((ranges = map.getFromRanges()).size(), 1);
+ assertEquals(ranges.get(0)[0], 2928);
+ assertEquals(ranges.get(0)[1], 3989);
+ assertEquals((ranges = map.getToRanges()).size(), 1);
+ assertEquals(ranges.get(0)[0], 1);
+ assertEquals(ranges.get(0)[1], 354);
+ }
+ else if ("UNIPROT|P0CE18".equals(mappedToName))
+ {
+ assertEquals((ranges = map.getFromRanges()).size(), 1);
+ assertEquals(ranges.get(0)[0], 4054);
+ assertEquals(ranges.get(0)[1], 4845);
+ assertEquals((ranges = map.getToRanges()).size(), 1);
+ assertEquals(ranges.get(0)[0], 1);
+ assertEquals(ranges.get(0)[1], 264);
+ }
+ else if ("UNIPROT|P0CE19".equals(mappedToName))
+ {
+ // join(7022..7502,1..437)
+ assertEquals((ranges = map.getFromRanges()).size(), 2);
+ assertEquals(ranges.get(0)[0], 7022);
+ assertEquals(ranges.get(0)[1], 7502);
+ assertEquals(ranges.get(1)[0], 1);
+ assertEquals(ranges.get(1)[1], 434);
+ assertEquals((ranges = map.getToRanges()).size(), 1);
+ assertEquals(ranges.get(0)[0], 1);
+ assertEquals(ranges.get(0)[1], 305);
+ }
+ else if ("UNIPROT|P0CE20".equals(mappedToName))
+ {
+ // complement(488..1480)
+ assertEquals((ranges = map.getFromRanges()).size(), 1);
+ assertEquals(ranges.get(0)[0], 1480);
+ assertEquals(ranges.get(0)[1], 491);
+ assertEquals((ranges = map.getToRanges()).size(), 1);
+ assertEquals(ranges.get(0)[0], 1);
+ assertEquals(ranges.get(0)[1], 330);
+ }
+ else if (!"UNIPROT|P0CE23".equals(mappedToName)
+ && !"UNIPROT|P10559".equals(mappedToName)
+ && !"UNIPROT|P10560".equals(mappedToName))
+ {
+ fail("Unexpected UNIPROT dbref to " + mappedToName);
+ }
+ }
+ }
+ assertEquals(uniprotCount, 8);
+ }
+
+ @Test(groups = "Functional")
+ public void testParse_codonStartNot1()
+ {
+ // TODO verify CDS-to-protein mapping for CDS with /codon_start=2
+ // example: https://www.ebi.ac.uk/ena/browser/api/embl/EU498516
+ }
+
+ /**
+ * Test for the case that the EMBL CDS has no UNIPROT xref. In this case
+ * Jalview should synthesize an xref to EMBLCDSPROTEIN in the hope this will
+ * allow Get Cross-References.
+ *
+ * @throws IOException
+ */
+ @Test(groups = "Functional")
+ public void testParse_noUniprotXref() throws IOException
+ {
+ // MN908947 cut down to 40BP, one CDS, length 5 peptide for test purposes
+ // plus an additional (invented) test case:
+ // - multi-line /product qualifier including escaped quotes
+ String data = "ID MN908947; SV 3; linear; genomic RNA; STD; VRL; 20 BP.\n"
+ + "DE Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1,\n"
+ + "FT CDS 3..17\n"
+ + "FT /protein_id=\"QHD43415.1\"\n"
+ + "FT /product=\"orf1ab polyprotein\n"
+ + "FT \"\"foobar\"\" \"\n"
+ + "FT /translation=\"MRKLD\n"
+ + "SQ Sequence 7496 BP; 2450 A; 1290 C; 1434 G; 2322 T; 0 other;\n"
+ + " ggatGcgtaa gttagacgaa attttgtctt tgcgcacaga 40\n";
+ FileParse fp = new FileParse(data, DataSourceType.PASTE);
+ EmblFlatFile parser = new EmblFlatFile(fp, "EmblTest");
+ parser.parse();
+ List<SequenceI> seqs = parser.getSeqs();
+ assertEquals(seqs.size(), 1);
+ SequenceI seq = seqs.get(0);
+ List<DBRefEntry> dbrefs = Arrays.asList(seq.getDBRefs());
- // todo: mappings to, and sequences for, UNIPROT proteins
+ /*
+ * dna should have dbref to itself, and to inferred EMBLCDSPROTEIN:QHD43415.1
+ */
+ assertEquals(dbrefs.size(), 2);
+
+ // dbref to self
+ DBRefEntry dbref = dbrefs.get(0);
+ assertEquals(dbref.getSource(), "EMBLTEST");
+ assertEquals(dbref.getAccessionId(), "MN908947");
+ Mapping mapping = dbref.getMap();
+ assertNull(mapping.getTo());
+ MapList map = mapping.getMap();
+ assertEquals(map.getFromLowest(), 1);
+ assertEquals(map.getFromHighest(), 40);
+ assertEquals(map.getToLowest(), 1);
+ assertEquals(map.getToHighest(), 40);
+ assertEquals(map.getFromRatio(), 1);
+ assertEquals(map.getToRatio(), 1);
+
+ // dbref to inferred EMBLCDSPROTEIN:
+ dbref = dbrefs.get(1);
+ assertEquals(dbref.getSource(), "EMBLCDSPROTEIN");
+ assertEquals(dbref.getAccessionId(), "QHD43415.1");
+ mapping = dbref.getMap();
+ SequenceI mapTo = mapping.getTo();
+ assertEquals(mapTo.getName(), "QHD43415.1");
+ // the /product qualifier transfers to protein product description
+ assertEquals(mapTo.getDescription(), "orf1ab polyprotein \"foobar\"");
+ assertEquals(mapTo.getSequenceAsString(), "MRKLD");
+ map = mapping.getMap();
+ assertEquals(map.getFromLowest(), 3);
+ assertEquals(map.getFromHighest(), 17);
+ assertEquals(map.getToLowest(), 1);
+ assertEquals(map.getToHighest(), 5);
+ assertEquals(map.getFromRatio(), 3);
+ assertEquals(map.getToRatio(), 1);
+ }
+
+ @Test(groups = "Functional")
+ public void testAdjustForProteinLength()
+ {
+ int[] exons = new int[] { 11, 15, 21, 25, 31, 38 }; // 18 bp
+
+ int[] exons_nostop = new int[] { 11, 15, 21, 25, 31, 35 }; // 15 bp
+ // exact length match:
+ assertSame(exons, EmblFlatFile.adjustForProteinLength(6, exons));
+
+ // trimmed if we assume exons include stop codon not in protein:
+ assertEquals(Arrays.toString(exons_nostop), Arrays.toString(EmblFlatFile.adjustForProteinLength(5, exons)));
+
+ // truncate last exon by 6bp
+ int[] truncated = EmblFlatFile.adjustForProteinLength(4, exons);
+ assertEquals("[11, 15, 21, 25, 31, 32]", Arrays.toString(truncated));
+
+ // remove last exon and truncate preceding by 1bp (so 3bp in total)
+ truncated = EmblFlatFile.adjustForProteinLength(3, exons);
+ assertEquals("[11, 15, 21, 24]", Arrays.toString(truncated));
+
+ // exact removal of exon case:
+ exons = new int[] { 11, 15, 21, 27, 33, 38 }; // 18 bp
+ truncated = EmblFlatFile.adjustForProteinLength(4, exons);
+ assertEquals("[11, 15, 21, 27]", Arrays.toString(truncated));
+
+ // what if exons are too short for protein?
+ truncated = EmblFlatFile.adjustForProteinLength(7, exons);
+ assertSame(exons, truncated);
+ }
+
+ @Test(groups = "Functional")
+ public void testRemoveQuotes()
+ {
+ assertNull(EmblFlatFile.removeQuotes(null));
+ assertEquals(EmblFlatFile.removeQuotes("No quotes here"), "No quotes here");
+ assertEquals(EmblFlatFile.removeQuotes("\"Enclosing quotes\""), "Enclosing quotes");
+ assertEquals(EmblFlatFile.removeQuotes("\"Escaped \"\"quotes\"\" example\""), "Escaped \"quotes\" example");
}
}
git://source.jalview.org / jalview.git / blobdiff