test/jalview/renderer/seqfeatures/FeatureRendererTest.java

   1 /*
   2  * Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
   3  * Copyright (C) $$Year-Rel$$ The Jalview Authors
   4  *
   5  * This file is part of Jalview.
   6  *
   7  * Jalview is free software: you can redistribute it and/or
   8  * modify it under the terms of the GNU General Public License
   9  * as published by the Free Software Foundation, either version 3
  10  * of the License, or (at your option) any later version.
  11  *
  12  * Jalview is distributed in the hope that it will be useful, but
  13  * WITHOUT ANY WARRANTY; without even the implied warranty
  14  * of MERCHANTABILITY or FITNESS FOR A PARTICULAR
  15  * PURPOSE.  See the GNU General Public License for more details.
  16  *
  17  * You should have received a copy of the GNU General Public License
  18  * along with Jalview.  If not, see <http://www.gnu.org/licenses/>.
  19  * The Jalview Authors are detailed in the 'AUTHORS' file.
  20  */
  21 package jalview.renderer.seqfeatures;
  22
  23 import static org.testng.Assert.assertEquals;
  24 import static org.testng.Assert.assertFalse;
  25 import static org.testng.Assert.assertNull;
  26 import static org.testng.Assert.assertTrue;
  27
  28 import jalview.api.AlignViewportI;
  29 import jalview.api.FeatureColourI;
  30 import jalview.datamodel.SequenceFeature;
  31 import jalview.datamodel.SequenceI;
  32 import jalview.datamodel.features.FeatureMatcher;
  33 import jalview.datamodel.features.FeatureMatcherSet;
  34 import jalview.datamodel.features.FeatureMatcherSetI;
  35 import jalview.gui.AlignFrame;
  36 import jalview.io.DataSourceType;
  37 import jalview.io.FileLoader;
  38 import jalview.schemes.FeatureColour;
  39 import jalview.util.matcher.Condition;
  40 import jalview.viewmodel.seqfeatures.FeatureRendererModel.FeatureSettingsBean;
  41
  42 import java.awt.Color;
  43 import java.util.ArrayList;
  44 import java.util.Arrays;
  45 import java.util.HashMap;
  46 import java.util.List;
  47 import java.util.Map;
  48
  49 import org.testng.annotations.Test;
  50
  51 public class FeatureRendererTest
  52 {
  53
  54   @Test(groups = "Functional")
  55   public void testFindAllFeatures()
  56   {
  57     String seqData = ">s1\nabcdef\n>s2\nabcdef\n>s3\nabcdef\n>s4\nabcdef\n";
  58     AlignFrame af = new FileLoader().LoadFileWaitTillLoaded(seqData,
  59             DataSourceType.PASTE);
  60     AlignViewportI av = af.getViewport();
  61     FeatureRenderer fr = new FeatureRenderer(av);
  62
  63     /*
  64      * with no features
  65      */
  66     fr.findAllFeatures(true);
  67     assertTrue(fr.getRenderOrder().isEmpty());
  68     assertTrue(fr.getFeatureGroups().isEmpty());
  69
  70     List<SequenceI> seqs = av.getAlignment().getSequences();
  71
  72     // add a non-positional feature - should be ignored by FeatureRenderer
  73     SequenceFeature sf1 = new SequenceFeature("Type", "Desc", 0, 0, 1f,
  74             "Group");
  75     seqs.get(0).addSequenceFeature(sf1);
  76     fr.findAllFeatures(true);
  77     // ? bug - types and groups added for non-positional features
  78     List<String> types = fr.getRenderOrder();
  79     List<String> groups = fr.getFeatureGroups();
  80     assertEquals(types.size(), 0);
  81     assertFalse(types.contains("Type"));
  82     assertEquals(groups.size(), 0);
  83     assertFalse(groups.contains("Group"));
  84
  85     // add some positional features
  86     seqs.get(1).addSequenceFeature(
  87             new SequenceFeature("Pfam", "Desc", 5, 9, 1f, "PfamGroup"));
  88     seqs.get(2).addSequenceFeature(
  89             new SequenceFeature("Pfam", "Desc", 14, 22, 2f, "RfamGroup"));
  90     // bug in findAllFeatures - group not checked for a known feature type
  91     seqs.get(2).addSequenceFeature(new SequenceFeature("Rfam", "Desc", 5, 9,
  92             Float.NaN, "RfamGroup"));
  93     // existing feature type with null group
  94     seqs.get(3).addSequenceFeature(
  95             new SequenceFeature("Rfam", "Desc", 5, 9, Float.NaN, null));
  96     // new feature type with null group
  97     seqs.get(3).addSequenceFeature(
  98             new SequenceFeature("Scop", "Desc", 5, 9, Float.NaN, null));
  99     // null value for type produces NullPointerException
 100     fr.findAllFeatures(true);
 101     types = fr.getRenderOrder();
 102     groups = fr.getFeatureGroups();
 103     assertEquals(types.size(), 3);
 104     assertFalse(types.contains("Type"));
 105     assertTrue(types.contains("Pfam"));
 106     assertTrue(types.contains("Rfam"));
 107     assertTrue(types.contains("Scop"));
 108     assertEquals(groups.size(), 2);
 109     assertFalse(groups.contains("Group"));
 110     assertTrue(groups.contains("PfamGroup"));
 111     assertTrue(groups.contains("RfamGroup"));
 112     assertFalse(groups.contains(null)); // null group is ignored
 113
 114     /*
 115      * check min-max values
 116      */
 117     Map<String, float[][]> minMax = fr.getMinMax();
 118     assertEquals(minMax.size(), 1); // non-positional and NaN not stored
 119     assertEquals(minMax.get("Pfam")[0][0], 1f); // positional min
 120     assertEquals(minMax.get("Pfam")[0][1], 2f); // positional max
 121
 122     // increase max for Pfam, add scores for Rfam
 123     seqs.get(0).addSequenceFeature(
 124             new SequenceFeature("Pfam", "Desc", 14, 22, 8f, "RfamGroup"));
 125     seqs.get(1).addSequenceFeature(
 126             new SequenceFeature("Rfam", "Desc", 5, 9, 6f, "RfamGroup"));
 127     fr.findAllFeatures(true);
 128     // note minMax is not a defensive copy, shouldn't expose this
 129     assertEquals(minMax.size(), 2);
 130     assertEquals(minMax.get("Pfam")[0][0], 1f);
 131     assertEquals(minMax.get("Pfam")[0][1], 8f);
 132     assertEquals(minMax.get("Rfam")[0][0], 6f);
 133     assertEquals(minMax.get("Rfam")[0][1], 6f);
 134
 135     /*
 136      * check render order (last is on top)
 137      */
 138     List<String> renderOrder = fr.getRenderOrder();
 139     assertEquals(renderOrder, Arrays.asList("Scop", "Rfam", "Pfam"));
 140
 141     /*
 142      * change render order (todo: an easier way)
 143      * nb here last comes first in the data array
 144      */
 145     FeatureSettingsBean[] data = new FeatureSettingsBean[3];
 146     FeatureColourI colour = new FeatureColour(Color.RED);
 147     data[0] = new FeatureSettingsBean("Rfam", colour, null, true);
 148     data[1] = new FeatureSettingsBean("Pfam", colour, null, false);
 149     data[2] = new FeatureSettingsBean("Scop", colour, null, false);
 150     fr.setFeaturePriority(data);
 151     assertEquals(fr.getRenderOrder(),
 152             Arrays.asList("Scop", "Pfam", "Rfam"));
 153     assertEquals(fr.getDisplayedFeatureTypes(), Arrays.asList("Rfam"));
 154
 155     /*
 156      * add a new feature type: should go on top of render order as visible,
 157      * other feature ordering and visibility should be unchanged
 158      */
 159     seqs.get(2).addSequenceFeature(
 160             new SequenceFeature("Metal", "Desc", 14, 22, 8f, "MetalGroup"));
 161     fr.findAllFeatures(true);
 162     assertEquals(fr.getRenderOrder(),
 163             Arrays.asList("Scop", "Pfam", "Rfam", "Metal"));
 164     assertEquals(fr.getDisplayedFeatureTypes(),
 165             Arrays.asList("Rfam", "Metal"));
 166   }
 167
 168   @Test(groups = "Functional")
 169   public void testFindFeaturesAtColumn()
 170   {
 171     String seqData = ">s1/4-29\n-ab--cdefghijklmnopqrstuvwxyz\n";
 172     AlignFrame af = new FileLoader().LoadFileWaitTillLoaded(seqData,
 173             DataSourceType.PASTE);
 174     AlignViewportI av = af.getViewport();
 175     FeatureRenderer fr = new FeatureRenderer(av);
 176     SequenceI seq = av.getAlignment().getSequenceAt(0);
 177
 178     /*
 179      * with no features
 180      */
 181     List<SequenceFeature> features = fr.findFeaturesAtColumn(seq, 3);
 182     assertTrue(features.isEmpty());
 183
 184     /*
 185      * add features
 186      */
 187     SequenceFeature sf1 = new SequenceFeature("Type1", "Desc", 0, 0, 1f,
 188             "Group"); // non-positional
 189     seq.addSequenceFeature(sf1);
 190     SequenceFeature sf2 = new SequenceFeature("Type2", "Desc", 8, 18, 1f,
 191             "Group1");
 192     seq.addSequenceFeature(sf2);
 193     SequenceFeature sf3 = new SequenceFeature("Type3", "Desc", 8, 18, 1f,
 194             "Group2");
 195     seq.addSequenceFeature(sf3);
 196     SequenceFeature sf4 = new SequenceFeature("Type3", "Desc", 8, 18, 1f,
 197             null); // null group is always treated as visible
 198     seq.addSequenceFeature(sf4);
 199
 200     /*
 201      * add contact features
 202      */
 203     SequenceFeature sf5 = new SequenceFeature("Disulphide Bond", "Desc", 7,
 204             15, 1f, "Group1");
 205     seq.addSequenceFeature(sf5);
 206     SequenceFeature sf6 = new SequenceFeature("Disulphide Bond", "Desc", 7,
 207             15, 1f, "Group2");
 208     seq.addSequenceFeature(sf6);
 209     SequenceFeature sf7 = new SequenceFeature("Disulphide Bond", "Desc", 7,
 210             15, 1f, null);
 211     seq.addSequenceFeature(sf7);
 212
 213     // feature spanning B--C
 214     SequenceFeature sf8 = new SequenceFeature("Type1", "Desc", 5, 6, 1f,
 215             "Group");
 216     seq.addSequenceFeature(sf8);
 217     // contact feature B/C
 218     SequenceFeature sf9 = new SequenceFeature("Disulphide Bond", "Desc", 5,
 219             6, 1f, "Group");
 220     seq.addSequenceFeature(sf9);
 221
 222     /*
 223      * let feature renderer discover features (and make visible)
 224      */
 225     fr.findAllFeatures(true);
 226     features = fr.findFeaturesAtColumn(seq, 15); // all positional
 227     assertEquals(features.size(), 6);
 228     assertTrue(features.contains(sf2));
 229     assertTrue(features.contains(sf3));
 230     assertTrue(features.contains(sf4));
 231     assertTrue(features.contains(sf5));
 232     assertTrue(features.contains(sf6));
 233     assertTrue(features.contains(sf7));
 234
 235     /*
 236      * at a non-contact position
 237      */
 238     features = fr.findFeaturesAtColumn(seq, 14);
 239     assertEquals(features.size(), 3);
 240     assertTrue(features.contains(sf2));
 241     assertTrue(features.contains(sf3));
 242     assertTrue(features.contains(sf4));
 243
 244     /*
 245      * make "Type2" not displayed
 246      */
 247     FeatureColourI colour = new FeatureColour(Color.RED);
 248     FeatureSettingsBean[] data = new FeatureSettingsBean[4];
 249     data[0] = new FeatureSettingsBean("Type1", colour, null, true);
 250     data[1] = new FeatureSettingsBean("Type2", colour, null, false);
 251     data[2] = new FeatureSettingsBean("Type3", colour, null, true);
 252     data[3] = new FeatureSettingsBean("Disulphide Bond", colour, null,
 253             true);
 254     fr.setFeaturePriority(data);
 255
 256     features = fr.findFeaturesAtColumn(seq, 15);
 257     assertEquals(features.size(), 5); // no sf2
 258     assertTrue(features.contains(sf3));
 259     assertTrue(features.contains(sf4));
 260     assertTrue(features.contains(sf5));
 261     assertTrue(features.contains(sf6));
 262     assertTrue(features.contains(sf7));
 263
 264     /*
 265      * make "Group2" not displayed
 266      */
 267     fr.setGroupVisibility("Group2", false);
 268
 269     features = fr.findFeaturesAtColumn(seq, 15);
 270     assertEquals(features.size(), 3); // no sf2, sf3, sf6
 271     assertTrue(features.contains(sf4));
 272     assertTrue(features.contains(sf5));
 273     assertTrue(features.contains(sf7));
 274
 275     // features 'at' a gap between b and c
 276     // - returns enclosing feature BC but not contact feature B/C
 277     features = fr.findFeaturesAtColumn(seq, 4);
 278     assertEquals(features.size(), 1);
 279     assertTrue(features.contains(sf8));
 280     features = fr.findFeaturesAtColumn(seq, 5);
 281     assertEquals(features.size(), 1);
 282     assertTrue(features.contains(sf8));
 283
 284     /*
 285      * give "Type3" features a graduated colour scheme
 286      * - first with no threshold
 287      */
 288     FeatureColourI gc = new FeatureColour(Color.yellow, Color.red, null, 0f,
 289             10f);
 290     fr.getFeatureColours().put("Type3", gc);
 291     features = fr.findFeaturesAtColumn(seq, 8);
 292     assertTrue(features.contains(sf4));
 293     // now with threshold > 2f - feature score of 1f is excluded
 294     gc.setAboveThreshold(true);
 295     gc.setThreshold(2f);
 296     features = fr.findFeaturesAtColumn(seq, 8);
 297     assertFalse(features.contains(sf4));
 298
 299     /*
 300      * make "Type3" graduated colour by attribute "AF"
 301      * - first with no attribute held - feature should be excluded
 302      */
 303     gc.setAttributeName("AF");
 304     features = fr.findFeaturesAtColumn(seq, 8);
 305     assertFalse(features.contains(sf4));
 306     // now with the attribute above threshold - should be included
 307     sf4.setValue("AF", "2.4");
 308     features = fr.findFeaturesAtColumn(seq, 8);
 309     assertTrue(features.contains(sf4));
 310     // now with the attribute below threshold - should be excluded
 311     sf4.setValue("AF", "1.4");
 312     features = fr.findFeaturesAtColumn(seq, 8);
 313     assertFalse(features.contains(sf4));
 314   }
 315
 316   @Test(groups = "Functional")
 317   public void testFilterFeaturesForDisplay()
 318   {
 319     String seqData = ">s1\nabcdef\n";
 320     AlignFrame af = new FileLoader().LoadFileWaitTillLoaded(seqData,
 321             DataSourceType.PASTE);
 322     AlignViewportI av = af.getViewport();
 323     FeatureRenderer fr = new FeatureRenderer(av);
 324
 325     List<SequenceFeature> features = new ArrayList<>();
 326     fr.filterFeaturesForDisplay(features); // empty list, does nothing
 327
 328     SequenceI seq = av.getAlignment().getSequenceAt(0);
 329     SequenceFeature sf1 = new SequenceFeature("Cath", "", 6, 8, Float.NaN,
 330             "group1");
 331     SequenceFeature sf2 = new SequenceFeature("Cath", "", 5, 11, 2f,
 332             "group2");
 333     SequenceFeature sf3 = new SequenceFeature("Cath", "", 5, 11, 3f,
 334             "group3");
 335     SequenceFeature sf4 = new SequenceFeature("Cath", "", 6, 8, 4f,
 336             "group4");
 337     SequenceFeature sf5 = new SequenceFeature("Cath", "", 6, 9, 5f,
 338             "group4");
 339     seq.addSequenceFeature(sf1);
 340     seq.addSequenceFeature(sf2);
 341     seq.addSequenceFeature(sf3);
 342     seq.addSequenceFeature(sf4);
 343     seq.addSequenceFeature(sf5);
 344
 345     fr.findAllFeatures(true);
 346
 347     features = seq.getSequenceFeatures();
 348     assertEquals(features.size(), 5);
 349     assertTrue(features.contains(sf1));
 350     assertTrue(features.contains(sf2));
 351     assertTrue(features.contains(sf3));
 352     assertTrue(features.contains(sf4));
 353     assertTrue(features.contains(sf5));
 354
 355     /*
 356      * filter out duplicate (co-located) features
 357      * note: which gets removed is not guaranteed
 358      */
 359     fr.filterFeaturesForDisplay(features);
 360     assertEquals(features.size(), 3);
 361     assertTrue(features.contains(sf1) || features.contains(sf4));
 362     assertFalse(features.contains(sf1) && features.contains(sf4));
 363     assertTrue(features.contains(sf2) || features.contains(sf3));
 364     assertFalse(features.contains(sf2) && features.contains(sf3));
 365     assertTrue(features.contains(sf5));
 366
 367     /*
 368      * features in hidden groups are removed
 369      */
 370     fr.setGroupVisibility("group2", false);
 371     fr.setGroupVisibility("group3", false);
 372     features = seq.getSequenceFeatures();
 373     fr.filterFeaturesForDisplay(features);
 374     assertEquals(features.size(), 2);
 375     assertTrue(features.contains(sf1) || features.contains(sf4));
 376     assertFalse(features.contains(sf1) && features.contains(sf4));
 377     assertFalse(features.contains(sf2));
 378     assertFalse(features.contains(sf3));
 379     assertTrue(features.contains(sf5));
 380
 381     /*
 382      * no filtering if transparency is applied
 383      */
 384     fr.setTransparency(0.5f);
 385     features = seq.getSequenceFeatures();
 386     fr.filterFeaturesForDisplay(features);
 387     assertEquals(features.size(), 5);
 388   }
 389
 390   @Test(groups = "Functional")
 391   public void testGetColour()
 392   {
 393     AlignFrame af = new FileLoader().LoadFileWaitTillLoaded(">s1\nABCD\n",
 394             DataSourceType.PASTE);
 395     AlignViewportI av = af.getViewport();
 396     FeatureRenderer fr = new FeatureRenderer(av);
 397
 398     /*
 399      * simple colour, feature type and group displayed
 400      */
 401     FeatureColourI fc = new FeatureColour(Color.red);
 402     fr.getFeatureColours().put("Cath", fc);
 403     SequenceFeature sf1 = new SequenceFeature("Cath", "", 6, 8, Float.NaN,
 404             "group1");
 405     assertEquals(fr.getColour(sf1), Color.red);
 406
 407     /*
 408      * hide feature type, then unhide
 409      * - feature type visibility should not affect the result
 410      */
 411     FeatureSettingsBean[] data = new FeatureSettingsBean[1];
 412     data[0] = new FeatureSettingsBean("Cath", fc, null, false);
 413     fr.setFeaturePriority(data);
 414     assertEquals(fr.getColour(sf1), Color.red);
 415     data[0] = new FeatureSettingsBean("Cath", fc, null, true);
 416     fr.setFeaturePriority(data);
 417     assertEquals(fr.getColour(sf1), Color.red);
 418
 419     /*
 420      * hide feature group, then unhide
 421      */
 422     fr.setGroupVisibility("group1", false);
 423     assertNull(fr.getColour(sf1));
 424     fr.setGroupVisibility("group1", true);
 425     assertEquals(fr.getColour(sf1), Color.red);
 426
 427     /*
 428      * graduated colour by score, no threshold, no score
 429      *
 430      */
 431     FeatureColourI gc = new FeatureColour(Color.yellow, Color.red,
 432             Color.green, 1f, 11f);
 433     fr.getFeatureColours().put("Cath", gc);
 434     assertEquals(fr.getColour(sf1), Color.green);
 435
 436     /*
 437      * graduated colour by score, no threshold, with score value
 438      */
 439     SequenceFeature sf2 = new SequenceFeature("Cath", "", 6, 8, 6f,
 440             "group1");
 441     // score 6 is half way from yellow(255, 255, 0) to red(255, 0, 0)
 442     Color expected = new Color(255, 128, 0);
 443     assertEquals(fr.getColour(sf2), expected);
 444
 445     /*
 446      * above threshold, score is above threshold - no change
 447      */
 448     gc.setAboveThreshold(true);
 449     gc.setThreshold(5f);
 450     assertEquals(fr.getColour(sf2), expected);
 451
 452     /*
 453      * threshold is min-max; now score 6 is 1/6 of the way from 5 to 11
 454      * or from yellow(255, 255, 0) to red(255, 0, 0)
 455      */
 456     gc = new FeatureColour(Color.yellow, Color.red, Color.green, 5f, 11f);
 457     fr.getFeatureColours().put("Cath", gc);
 458     gc.setAutoScaled(false); // this does little other than save a checkbox setting!
 459     assertEquals(fr.getColour(sf2), new Color(255, 213, 0));
 460
 461     /*
 462      * feature score is below threshold - no colour
 463      */
 464     gc.setAboveThreshold(true);
 465     gc.setThreshold(7f);
 466     assertNull(fr.getColour(sf2));
 467
 468     /*
 469      * feature score is above threshold - no colour
 470      */
 471     gc.setBelowThreshold(true);
 472     gc.setThreshold(3f);
 473     assertNull(fr.getColour(sf2));
 474
 475     /*
 476      * colour by feature attribute value
 477      * first with no value held
 478      */
 479     gc = new FeatureColour(Color.yellow, Color.red, Color.green, 1f, 11f);
 480     fr.getFeatureColours().put("Cath", gc);
 481     gc.setAttributeName("AF");
 482     assertEquals(fr.getColour(sf2), Color.green);
 483
 484     // with non-numeric attribute value
 485     sf2.setValue("AF", "Five");
 486     assertEquals(fr.getColour(sf2), Color.green);
 487
 488     // with numeric attribute value
 489     sf2.setValue("AF", "6");
 490     assertEquals(fr.getColour(sf2), expected);
 491
 492     // with numeric value outwith threshold
 493     gc.setAboveThreshold(true);
 494     gc.setThreshold(10f);
 495     assertNull(fr.getColour(sf2));
 496
 497     // with filter on AF < 4
 498     gc.setAboveThreshold(false);
 499     assertEquals(fr.getColour(sf2), expected);
 500     FeatureMatcherSetI filter = new FeatureMatcherSet();
 501     filter.and(FeatureMatcher.byAttribute(Condition.LT, "4.0", "AF"));
 502     fr.setFeatureFilter("Cath", filter);
 503     assertNull(fr.getColour(sf2));
 504
 505     // with filter on 'Consequence contains missense'
 506     filter = new FeatureMatcherSet();
 507     filter.and(FeatureMatcher.byAttribute(Condition.Contains, "missense",
 508             "Consequence"));
 509     fr.setFeatureFilter("Cath", filter);
 510     // if feature has no Consequence attribute, no colour
 511     assertNull(fr.getColour(sf2));
 512     // if attribute does not match filter, no colour
 513     sf2.setValue("Consequence", "Synonymous");
 514     assertNull(fr.getColour(sf2));
 515     // attribute matches filter
 516     sf2.setValue("Consequence", "Missense variant");
 517     assertEquals(fr.getColour(sf2), expected);
 518
 519     // with filter on CSQ:Feature contains "ENST01234"
 520     filter = new FeatureMatcherSet();
 521     filter.and(FeatureMatcher.byAttribute(Condition.Matches, "ENST01234",
 522             "CSQ", "Feature"));
 523     fr.setFeatureFilter("Cath", filter);
 524     // if feature has no CSQ data, no colour
 525     assertNull(fr.getColour(sf2));
 526     // if CSQ data does not include Feature, no colour
 527     Map<String, String> csqData = new HashMap<>();
 528     csqData.put("BIOTYPE", "Transcript");
 529     sf2.setValue("CSQ", csqData);
 530     assertNull(fr.getColour(sf2));
 531     // if attribute does not match filter, no colour
 532     csqData.put("Feature", "ENST9876");
 533     assertNull(fr.getColour(sf2));
 534     // attribute matches filter
 535     csqData.put("Feature", "ENST01234");
 536     assertEquals(fr.getColour(sf2), expected);
 537   }
 538
 539   @Test(groups = "Functional")
 540   public void testIsVisible()
 541   {
 542     String seqData = ">s1\nMLQGIFPRS\n";
 543     AlignFrame af = new FileLoader().LoadFileWaitTillLoaded(seqData,
 544             DataSourceType.PASTE);
 545     AlignViewportI av = af.getViewport();
 546     FeatureRenderer fr = new FeatureRenderer(av);
 547     SequenceI seq = av.getAlignment().getSequenceAt(0);
 548     SequenceFeature sf = new SequenceFeature("METAL", "Desc", 10, 10, 1f,
 549             "Group");
 550     sf.setValue("AC", "11");
 551     sf.setValue("CLIN_SIG", "Likely Pathogenic");
 552     seq.addSequenceFeature(sf);
 553
 554     assertFalse(fr.isVisible(null));
 555
 556     /*
 557      * initial state FeatureRenderer hasn't 'found' feature
 558      * and so its feature type has not yet been set visible
 559      */
 560     assertFalse(fr.getDisplayedFeatureCols().containsKey("METAL"));
 561     assertFalse(fr.isVisible(sf));
 562
 563     fr.findAllFeatures(true);
 564     assertTrue(fr.isVisible(sf));
 565
 566     /*
 567      * feature group not visible
 568      */
 569     fr.setGroupVisibility("Group", false);
 570     assertFalse(fr.isVisible(sf));
 571     fr.setGroupVisibility("Group", true);
 572     assertTrue(fr.isVisible(sf));
 573
 574     /*
 575      * feature score outwith colour threshold (score > 2)
 576      */
 577     FeatureColourI fc = new FeatureColour(Color.white, Color.black,
 578             Color.white, 0, 10);
 579     fc.setAboveThreshold(true);
 580     fc.setThreshold(2f);
 581     fr.setColour("METAL", fc);
 582     assertFalse(fr.isVisible(sf)); // score 1 is not above threshold 2
 583     fc.setBelowThreshold(true);
 584     assertTrue(fr.isVisible(sf)); // score 1 is below threshold 2
 585
 586     /*
 587      * colour with threshold on attribute AC (value is 11)
 588      */
 589     fc.setAttributeName("AC");
 590     assertFalse(fr.isVisible(sf)); // value 11 is not below threshold 2
 591     fc.setAboveThreshold(true);
 592     assertTrue(fr.isVisible(sf)); // value 11 is above threshold 2
 593
 594     fc.setAttributeName("AF"); // attribute AF is absent in sf
 595     assertTrue(fr.isVisible(sf)); // feature is not excluded by threshold
 596
 597     FeatureMatcherSetI filter = new FeatureMatcherSet();
 598     filter.and(FeatureMatcher.byAttribute(Condition.Contains, "pathogenic",
 599             "CLIN_SIG"));
 600     fr.setFeatureFilter("METAL", filter);
 601     assertTrue(fr.isVisible(sf)); // feature matches filter
 602     filter.and(FeatureMatcher.byScore(Condition.LE, "0.4"));
 603     assertFalse(fr.isVisible(sf)); // feature doesn't match filter
 604   }
 605 }