2 * Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
3 * Copyright (C) $$Year-Rel$$ The Jalview Authors
5 * This file is part of Jalview.
7 * Jalview is free software: you can redistribute it and/or
8 * modify it under the terms of the GNU General Public License
9 * as published by the Free Software Foundation, either version 3
10 * of the License, or (at your option) any later version.
12 * Jalview is distributed in the hope that it will be useful, but
13 * WITHOUT ANY WARRANTY; without even the implied warranty
14 * of MERCHANTABILITY or FITNESS FOR A PARTICULAR
15 * PURPOSE. See the GNU General Public License for more details.
17 * You should have received a copy of the GNU General Public License
18 * along with Jalview. If not, see <http://www.gnu.org/licenses/>.
19 * The Jalview Authors are detailed in the 'AUTHORS' file.
21 package jalview.datamodel;
23 import jalview.util.MapList;
24 import jalview.util.MappingUtils;
26 import java.util.AbstractList;
27 import java.util.ArrayList;
28 import java.util.List;
31 * Stores mapping between the columns of a protein alignment and a DNA alignment
32 * and a list of individual codon to amino acid mappings between sequences.
34 public class AlignedCodonFrame
38 * Data bean to hold mappings from one sequence to another
40 public class SequenceToSequenceMapping
42 private SequenceI fromSeq;
44 private Mapping mapping;
46 SequenceToSequenceMapping(SequenceI from, Mapping map)
53 * Readable representation for debugging only, not guaranteed not to change
56 public String toString()
58 return String.format("From %s %s", fromSeq.getName(),
63 * Returns a hashCode derived from the hashcodes of the mappings and fromSeq
65 * @see SequenceToSequenceMapping#hashCode()
70 return (fromSeq == null ? 0 : fromSeq.hashCode() * 31)
75 * Answers true if the objects hold the same mapping between the same two
81 public boolean equals(Object obj)
83 if (!(obj instanceof SequenceToSequenceMapping))
87 SequenceToSequenceMapping that = (SequenceToSequenceMapping) obj;
88 if (this.mapping == null)
90 return that.mapping == null;
92 // TODO: can simplify by asserting fromSeq is a dataset sequence
93 return (this.fromSeq == that.fromSeq
94 || (this.fromSeq != null && that.fromSeq != null
95 && this.fromSeq.getDatasetSequence() != null
96 && this.fromSeq.getDatasetSequence() == that.fromSeq
97 .getDatasetSequence()))
98 && this.mapping.equals(that.mapping);
101 public SequenceI getFromSeq()
106 public Mapping getMapping()
112 * Returns true if the mapping covers the full length of the given sequence.
113 * This allows us to distinguish the CDS that codes for a protein from
114 * another overlapping CDS in the parent dna sequence.
119 public boolean covers(SequenceI seq)
121 return covers(seq,false,false);
126 * @param localCover - when true - compare extent of seq's dataset sequence rather than the local extent
127 * @param either - when true coverage is required for either seq or the mapped sequence
128 * @return true if mapping covers full length of given sequence (or the other if either==true)
130 public boolean covers(SequenceI seq, boolean localCover,boolean either)
132 List<int[]> mappedRanges = null,otherRanges=null;
133 MapList mapList = mapping.getMap();
134 int mstart=seq.getStart(),mend=seq.getEnd(),ostart,oend;
136 if (fromSeq == seq || fromSeq == seq.getDatasetSequence())
138 if (localCover && fromSeq !=seq)
140 mstart=fromSeq.getStart();
141 mend=fromSeq.getEnd();
143 mappedRanges = mapList.getFromRanges();
144 otherRanges=mapList.getToRanges();
145 ostart=mapping.to.getStart();
146 oend=mapping.to.getEnd();
148 else if (mapping.to == seq || mapping.to == seq.getDatasetSequence())
150 if (localCover && mapping.to !=seq)
152 mstart=mapping.to.getStart();
153 mend=mapping.to.getEnd();
155 mappedRanges = mapList.getToRanges();
156 otherRanges=mapList.getFromRanges();
157 ostart=fromSeq.getStart();
158 oend=fromSeq.getEnd();
166 * check that each mapped range lieS with the sequence range
167 * (necessary for circular CDS - example EMBL:J03321:AAA91567)
168 * and mapped length covers (at least) sequence length
170 int length = countRange(mappedRanges,mstart,mend);
174 // add 1 to mapped length to allow for a mapped stop codon
175 if (length + 1 >= (mend - mstart + 1))
182 // also check coverage of the other range
183 length = countRange(otherRanges, ostart, oend);
186 if (length + 1 >= (oend - ostart + 1))
194 private int countRange(List<int[]> mappedRanges,int mstart,int mend)
197 for (int[] range : mappedRanges)
199 int from = Math.min(range[0], range[1]);
200 int to = Math.max(range[0], range[1]);
201 if (from < mstart || to > mend)
205 length += (to - from + 1);
212 private List<SequenceToSequenceMapping> mappings;
217 public AlignedCodonFrame()
219 mappings = new ArrayList<>();
223 * Adds a mapping between the dataset sequences for the associated dna and
224 * protein sequence objects
230 public void addMap(SequenceI dnaseq, SequenceI aaseq, MapList map)
232 addMap(dnaseq, aaseq, map, null);
236 * Adds a mapping between the dataset sequences for the associated dna and
237 * protein sequence objects
244 public void addMap(SequenceI dnaseq, SequenceI aaseq, MapList map,
247 // JBPNote DEBUG! THIS !
248 // dnaseq.transferAnnotation(aaseq, mp);
249 // aaseq.transferAnnotation(dnaseq, new Mapping(map.getInverse()));
251 SequenceI fromSeq = (dnaseq.getDatasetSequence() == null) ? dnaseq
252 : dnaseq.getDatasetSequence();
253 SequenceI toSeq = (aaseq.getDatasetSequence() == null) ? aaseq
254 : aaseq.getDatasetSequence();
257 * if we already hold a mapping between these sequences, just add to it
258 * note that 'adding' a duplicate map does nothing; this protects against
259 * creating duplicate mappings in AlignedCodonFrame
261 for (SequenceToSequenceMapping ssm : mappings)
263 if (ssm.fromSeq == fromSeq && ssm.mapping.to == toSeq)
265 ssm.mapping.map.addMapList(map);
271 * otherwise, add a new sequence mapping
273 Mapping mp = new Mapping(toSeq, map);
274 mp.setMappedFromId(mapFromId);
275 mappings.add(new SequenceToSequenceMapping(fromSeq, mp));
278 public SequenceI[] getdnaSeqs()
280 // TODO return a list instead?
282 List<SequenceI> seqs = new ArrayList<>();
283 for (SequenceToSequenceMapping ssm : mappings)
285 seqs.add(ssm.fromSeq);
287 return seqs.toArray(new SequenceI[seqs.size()]);
290 public SequenceI[] getAaSeqs()
292 // TODO not used - remove?
293 List<SequenceI> seqs = new ArrayList<>();
294 for (SequenceToSequenceMapping ssm : mappings)
296 seqs.add(ssm.mapping.to);
298 return seqs.toArray(new SequenceI[seqs.size()]);
301 public MapList[] getdnaToProt()
303 List<MapList> maps = new ArrayList<>();
304 for (SequenceToSequenceMapping ssm : mappings)
306 maps.add(ssm.mapping.map);
308 return maps.toArray(new MapList[maps.size()]);
311 public Mapping[] getProtMappings()
313 List<Mapping> maps = new ArrayList<>();
314 for (SequenceToSequenceMapping ssm : mappings)
316 maps.add(ssm.mapping);
318 return maps.toArray(new Mapping[maps.size()]);
322 * Returns the first mapping found which is to or from the given sequence, or
323 * null if none is found
328 public Mapping getMappingForSequence(SequenceI seq)
330 SequenceI seqDs = seq.getDatasetSequence();
331 seqDs = seqDs != null ? seqDs : seq;
333 for (SequenceToSequenceMapping ssm : mappings)
335 if (ssm.fromSeq == seqDs || ssm.mapping.to == seqDs)
344 * Return the corresponding aligned or dataset aa sequence for given dna
345 * sequence, null if not found.
350 public SequenceI getAaForDnaSeq(SequenceI dnaSeqRef)
352 SequenceI dnads = dnaSeqRef.getDatasetSequence();
353 for (SequenceToSequenceMapping ssm : mappings)
355 if (ssm.fromSeq == dnaSeqRef || ssm.fromSeq == dnads)
357 return ssm.mapping.to;
364 * Return the corresponding aligned or dataset dna sequence for given amino
365 * acid sequence, or null if not found. returns the sequence from the first
366 * mapping found that involves the protein sequence.
371 public SequenceI getDnaForAaSeq(SequenceI aaSeqRef)
373 SequenceI aads = aaSeqRef.getDatasetSequence();
374 for (SequenceToSequenceMapping ssm : mappings)
376 if (ssm.mapping.to == aaSeqRef || ssm.mapping.to == aads)
385 * test to see if codon frame involves seq in any way
388 * a nucleotide or protein sequence
389 * @return true if a mapping exists to or from this sequence to any translated
392 public boolean involvesSequence(SequenceI seq)
394 return getAaForDnaSeq(seq) != null || getDnaForAaSeq(seq) != null;
398 * Add search results for regions in other sequences that translate or are
399 * translated from a particular position in seq
405 * where highlighted regions go
407 public void markMappedRegion(SequenceI seq, int index,
408 SearchResultsI results)
411 SequenceI ds = seq.getDatasetSequence();
412 for (SequenceToSequenceMapping ssm : mappings)
414 if (ssm.fromSeq == seq || ssm.fromSeq == ds)
416 codon = ssm.mapping.map.locateInTo(index, index);
419 for (int i = 0; i < codon.length; i += 2)
421 results.addResult(ssm.mapping.to, codon[i], codon[i + 1]);
425 else if (ssm.mapping.to == seq || ssm.mapping.to == ds)
428 codon = ssm.mapping.map.locateInFrom(index, index);
431 for (int i = 0; i < codon.length; i += 2)
433 results.addResult(ssm.fromSeq, codon[i], codon[i + 1]);
442 * Returns the DNA codon positions (base 1) for the given position (base 1) in
443 * a mapped protein sequence, or null if no mapping is found.
445 * Intended for use in aligning cDNA to match aligned protein. Only the first
446 * mapping found is returned, so not suitable for use if multiple protein
447 * sequences are mapped to the same cDNA (but aligning cDNA as protein is
448 * ill-defined for this case anyway).
451 * the DNA dataset sequence
453 * residue position (base 1) in a protein sequence
456 public int[] getDnaPosition(SequenceI seq, int aaPos)
459 * Adapted from markMappedRegion().
463 for (SequenceToSequenceMapping ssm : mappings)
465 if (ssm.fromSeq == seq)
467 ml = getdnaToProt()[i];
472 return ml == null ? null : ml.locateInFrom(aaPos, aaPos);
476 * Convenience method to return the first aligned sequence in the given
477 * alignment whose dataset has a mapping with the given (aligned or dataset)
485 public SequenceI findAlignedSequence(SequenceI seq, AlignmentI al)
487 return findAlignedSequence(seq, al, null);
490 * Convenience method to return the first aligned sequence in the given
491 * alignment whose dataset has a mapping with the given (aligned or dataset)
492 * sequence, and optionally the mapping that relates them
496 * @param map - list to add the mapping to
497 * @return sequence from al that maps to seq
499 public SequenceI findAlignedSequence(SequenceI seq, AlignmentI al,List<SequenceToSequenceMapping> map)
502 * Search mapped protein ('to') sequences first.
504 for (SequenceToSequenceMapping ssm : mappings)
506 int mStart=ssm.getMapping().getMap().getFromLowest(),mEnd=ssm.getMapping().map.getFromHighest();
507 if ((ssm.fromSeq == seq || ssm.fromSeq == seq.getDatasetSequence())
508 && seq.getStart()>=mStart && seq.getEnd()<=mEnd)
510 for (SequenceI sourceAligned : al.getSequences())
512 if (ssm.covers(sourceAligned,true,false))
518 return sourceAligned;
525 * Then try mapped dna sequences.
527 for (SequenceToSequenceMapping ssm : mappings)
529 int mStart=ssm.getMapping().getMap().getToLowest(),mEnd=ssm.getMapping().map.getToHighest();
530 if ((ssm.mapping.to == seq
531 || ssm.mapping.to == seq.getDatasetSequence())
532 && seq.getStart()>=mStart && seq.getEnd()<=mEnd)
534 for (SequenceI sourceAligned : al.getSequences())
536 if (ssm.covers(sourceAligned,true,true))
542 return sourceAligned;
552 * Returns the region in the target sequence's dataset that is mapped to the
553 * given position (base 1) in the query sequence's dataset. The region is a
554 * set of start/end position pairs.
561 public int[] getMappedRegion(SequenceI target, SequenceI query,
564 SequenceI targetDs = target.getDatasetSequence() == null ? target
565 : target.getDatasetSequence();
566 SequenceI queryDs = query.getDatasetSequence() == null ? query
567 : query.getDatasetSequence();
568 if (targetDs == null || queryDs == null /*|| dnaToProt == null*/)
572 for (SequenceToSequenceMapping ssm : mappings)
575 * try mapping from target to query
577 if (ssm.fromSeq == targetDs && ssm.mapping.to == queryDs)
579 int[] codon = ssm.mapping.map.locateInFrom(queryPos, queryPos);
586 * else try mapping from query to target
588 else if (ssm.fromSeq == queryDs && ssm.mapping.to == targetDs)
590 int[] codon = ssm.mapping.map.locateInTo(queryPos, queryPos);
601 * Returns the mapped DNA codons for the given position in a protein sequence,
602 * or null if no mapping is found. Returns a list of (e.g.) ['g', 'c', 't']
603 * codons. There may be more than one codon mapped to the protein if (for
604 * example), there are mappings to cDNA variants.
607 * the peptide dataset sequence
609 * residue position (base 1) in the peptide sequence
612 public List<char[]> getMappedCodons(SequenceI protein, int aaPos)
615 SequenceI dnaSeq = null;
616 List<char[]> result = new ArrayList<>();
618 for (SequenceToSequenceMapping ssm : mappings)
620 if (ssm.mapping.to == protein
621 && ssm.mapping.getMap().getFromRatio() == 3)
623 ml = ssm.mapping.map;
624 dnaSeq = ssm.fromSeq;
626 int[] codonPos = ml.locateInFrom(aaPos, aaPos);
627 if (codonPos == null)
633 * Read off the mapped nucleotides (converting to position base 0)
635 codonPos = MappingUtils.flattenRanges(codonPos);
636 int start = dnaSeq.getStart();
637 char c1 = dnaSeq.getCharAt(codonPos[0] - start);
638 char c2 = dnaSeq.getCharAt(codonPos[1] - start);
639 char c3 = dnaSeq.getCharAt(codonPos[2] - start);
640 result.add(new char[] { c1, c2, c3 });
643 return result.isEmpty() ? null : result;
647 * Returns any mappings found which are from the given sequence, and to
648 * distinct sequences.
653 public List<Mapping> getMappingsFromSequence(SequenceI seq)
655 List<Mapping> result = new ArrayList<>();
656 List<SequenceI> related = new ArrayList<>();
657 SequenceI seqDs = seq.getDatasetSequence();
658 seqDs = seqDs != null ? seqDs : seq;
660 for (SequenceToSequenceMapping ssm : mappings)
662 final Mapping mapping = ssm.mapping;
663 if (ssm.fromSeq == seqDs)
665 if (!related.contains(mapping.to))
668 related.add(mapping.to);
676 * Test whether the given sequence is substitutable for one or more dummy
677 * sequences in this mapping
683 public boolean isRealisableWith(SequenceI seq)
685 return realiseWith(seq, false) > 0;
689 * Replace any matchable mapped dummy sequences with the given real one.
690 * Returns the count of sequence mappings instantiated.
695 public int realiseWith(SequenceI seq)
697 return realiseWith(seq, true);
701 * Returns the number of mapped dummy sequences that could be replaced with
702 * the given real sequence.
707 * if true, performs replacements, else only counts
710 protected int realiseWith(SequenceI seq, boolean doUpdate)
712 SequenceI ds = seq.getDatasetSequence() != null
713 ? seq.getDatasetSequence()
718 * check for replaceable DNA ('map from') sequences
720 for (SequenceToSequenceMapping ssm : mappings)
722 SequenceI dna = ssm.fromSeq;
723 if (dna instanceof SequenceDummy
724 && dna.getName().equals(ds.getName()))
726 Mapping mapping = ssm.mapping;
727 int mapStart = mapping.getMap().getFromLowest();
728 int mapEnd = mapping.getMap().getFromHighest();
729 boolean mappable = couldRealiseSequence(dna, ds, mapStart, mapEnd);
735 // TODO: new method ? ds.realise(dna);
736 // might want to copy database refs as well
737 ds.setSequenceFeatures(dna.getSequenceFeatures());
740 System.out.println("Realised mapped sequence " + ds.getName());
746 * check for replaceable protein ('map to') sequences
748 Mapping mapping = ssm.mapping;
749 SequenceI prot = mapping.getTo();
750 int mapStart = mapping.getMap().getToLowest();
751 int mapEnd = mapping.getMap().getToHighest();
752 boolean mappable = couldRealiseSequence(prot, ds, mapStart, mapEnd);
758 // TODO: new method ? ds.realise(dna);
759 // might want to copy database refs as well
760 ds.setSequenceFeatures(dna.getSequenceFeatures());
761 ssm.mapping.setTo(ds);
769 * Helper method to test whether a 'real' sequence could replace a 'dummy'
770 * sequence in the map. The criteria are that they have the same name, and
771 * that the mapped region overlaps the candidate sequence.
779 protected static boolean couldRealiseSequence(SequenceI existing,
780 SequenceI replacement, int mapStart, int mapEnd)
782 if (existing instanceof SequenceDummy
783 && !(replacement instanceof SequenceDummy)
784 && existing.getName().equals(replacement.getName()))
786 int start = replacement.getStart();
787 int end = replacement.getEnd();
788 boolean mappingOverlapsSequence = (mapStart >= start
789 && mapStart <= end) || (mapEnd >= start && mapEnd <= end);
790 if (mappingOverlapsSequence)
799 * Change any mapping to the given sequence to be to its dataset sequence
800 * instead. For use when mappings are created before their referenced
801 * sequences are instantiated, for example when parsing GFF data.
805 public void updateToDataset(SequenceI seq)
807 if (seq == null || seq.getDatasetSequence() == null)
811 SequenceI ds = seq.getDatasetSequence();
813 for (SequenceToSequenceMapping ssm : mappings)
818 if (ssm.fromSeq == seq)
826 if (ssm.mapping.to == seq)
834 * Answers true if this object contains no mappings
838 public boolean isEmpty()
840 return mappings.isEmpty();
844 * Method for debug / inspection purposes only, may change in future
847 public String toString()
849 return mappings == null ? "null" : mappings.toString();
853 * Returns the first mapping found that is between 'fromSeq' and 'toSeq', or
857 * aligned or dataset sequence
859 * aligned or dataset sequence
862 public Mapping getMappingBetween(SequenceI fromSeq, SequenceI toSeq)
864 SequenceI dssFrom = fromSeq.getDatasetSequence() == null ? fromSeq
865 : fromSeq.getDatasetSequence();
866 SequenceI dssTo = toSeq.getDatasetSequence() == null ? toSeq
867 : toSeq.getDatasetSequence();
869 for (SequenceToSequenceMapping mapping : mappings)
871 SequenceI from = mapping.fromSeq;
872 SequenceI to = mapping.mapping.to;
873 if ((from == dssFrom && to == dssTo)
874 || (from == dssTo && to == dssFrom))
876 return mapping.mapping;
883 * Returns a hashcode derived from the list of sequence mappings
885 * @see SequenceToSequenceMapping#hashCode()
886 * @see AbstractList#hashCode()
889 public int hashCode()
891 return this.mappings.hashCode();
895 * Two AlignedCodonFrame objects are equal if they hold the same ordered list
898 * @see SequenceToSequenceMapping#equals
901 public boolean equals(Object obj)
903 if (!(obj instanceof AlignedCodonFrame))
907 return this.mappings.equals(((AlignedCodonFrame) obj).mappings);
910 public List<SequenceToSequenceMapping> getMappings()
916 * Returns the first mapping found which is between the two given sequences,
917 * and covers the full extent of both.
923 public SequenceToSequenceMapping getCoveringMapping(SequenceI seq1,
926 for (SequenceToSequenceMapping mapping : mappings)
928 if (mapping.covers(seq2) && mapping.covers(seq1))