JAL-3438 spotless for 2.11.2.0
[jalview.git] / src / jalview / appletgui / APopupMenu.java
index eba1200..b860a36 100644 (file)
@@ -1,6 +1,6 @@
 /*
- * Jalview - A Sequence Alignment Editor and Viewer (Version 2.8.2)
- * Copyright (C) 2014 The Jalview Authors
+ * Jalview - A Sequence Alignment Editor and Viewer ($$Version-Rel$$)
+ * Copyright (C) $$Year-Rel$$ The Jalview Authors
  * 
  * This file is part of Jalview.
  * 
  */
 package jalview.appletgui;
 
-import java.util.*;
-
-import java.awt.*;
-import java.awt.event.*;
-
-import jalview.analysis.*;
-import jalview.commands.*;
-import jalview.datamodel.*;
-import jalview.schemes.*;
-import jalview.util.MessageManager;
-import jalview.util.UrlLink;
+import java.awt.CheckboxMenuItem;
+import java.awt.Frame;
+import java.awt.Menu;
+import java.awt.MenuItem;
+import java.awt.event.ActionEvent;
+import java.awt.event.ActionListener;
+import java.awt.event.ItemEvent;
+import java.awt.event.ItemListener;
+import java.util.ArrayList;
+import java.util.Arrays;
+import java.util.Collection;
+import java.util.Collections;
+import java.util.LinkedHashMap;
+import java.util.List;
+import java.util.Map;
+import java.util.SortedMap;
+import java.util.TreeMap;
+import java.util.Vector;
+
+import jalview.analysis.AAFrequency;
+import jalview.analysis.AlignmentAnnotationUtils;
+import jalview.analysis.AlignmentUtils;
+import jalview.analysis.Conservation;
+import jalview.bin.JalviewLite;
+import jalview.commands.ChangeCaseCommand;
+import jalview.commands.EditCommand;
+import jalview.commands.EditCommand.Action;
+import jalview.datamodel.AlignmentAnnotation;
+import jalview.datamodel.AlignmentI;
+import jalview.datamodel.PDBEntry;
+import jalview.datamodel.SequenceFeature;
+import jalview.datamodel.SequenceGroup;
+import jalview.datamodel.SequenceI;
 import jalview.io.AppletFormatAdapter;
+import jalview.io.DataSourceType;
+import jalview.io.FileFormatI;
+import jalview.io.FileFormats;
 import jalview.io.SequenceAnnotationReport;
+import jalview.renderer.ResidueShader;
+import jalview.renderer.ResidueShaderI;
+import jalview.schemes.Blosum62ColourScheme;
+import jalview.schemes.BuriedColourScheme;
+import jalview.schemes.ClustalxColourScheme;
+import jalview.schemes.HelixColourScheme;
+import jalview.schemes.HydrophobicColourScheme;
+import jalview.schemes.JalviewColourScheme;
+import jalview.schemes.NucleotideColourScheme;
+import jalview.schemes.PIDColourScheme;
+import jalview.schemes.PurinePyrimidineColourScheme;
+import jalview.schemes.StrandColourScheme;
+import jalview.schemes.TaylorColourScheme;
+import jalview.schemes.TurnColourScheme;
+import jalview.schemes.ZappoColourScheme;
+import jalview.util.MessageManager;
+import jalview.util.UrlLink;
 
-public class APopupMenu extends java.awt.PopupMenu implements
-        ActionListener, ItemListener
+public class APopupMenu extends java.awt.PopupMenu
+        implements ActionListener, ItemListener
 {
   Menu groupMenu = new Menu();
 
   MenuItem editGroupName = new MenuItem();
 
-  protected MenuItem clustalColour = new MenuItem();
+  CheckboxMenuItem noColour = new CheckboxMenuItem();
 
-  protected MenuItem zappoColour = new MenuItem();
+  protected CheckboxMenuItem clustalColour = new CheckboxMenuItem();
 
-  protected MenuItem taylorColour = new MenuItem();
+  protected CheckboxMenuItem zappoColour = new CheckboxMenuItem();
 
-  protected MenuItem hydrophobicityColour = new MenuItem();
+  protected CheckboxMenuItem taylorColour = new CheckboxMenuItem();
 
-  protected MenuItem helixColour = new MenuItem();
+  protected CheckboxMenuItem hydrophobicityColour = new CheckboxMenuItem();
 
-  protected MenuItem strandColour = new MenuItem();
+  protected CheckboxMenuItem helixColour = new CheckboxMenuItem();
 
-  protected MenuItem turnColour = new MenuItem();
+  protected CheckboxMenuItem strandColour = new CheckboxMenuItem();
 
-  protected MenuItem buriedColour = new MenuItem();
+  protected CheckboxMenuItem turnColour = new CheckboxMenuItem();
 
-  protected CheckboxMenuItem abovePIDColour = new CheckboxMenuItem();
+  protected CheckboxMenuItem buriedColour = new CheckboxMenuItem();
+
+  protected CheckboxMenuItem PIDColour = new CheckboxMenuItem();
+
+  protected CheckboxMenuItem BLOSUM62Colour = new CheckboxMenuItem();
+
+  CheckboxMenuItem nucleotideColour = new CheckboxMenuItem();
+
+  CheckboxMenuItem purinePyrimidineColour = new CheckboxMenuItem();
 
   protected MenuItem userDefinedColour = new MenuItem();
 
-  protected MenuItem PIDColour = new MenuItem();
+  protected CheckboxMenuItem abovePIDColour = new CheckboxMenuItem();
 
-  protected MenuItem BLOSUM62Colour = new MenuItem();
+  MenuItem modifyPID = new MenuItem();
 
-  MenuItem noColourmenuItem = new MenuItem();
+  protected CheckboxMenuItem conservationColour = new CheckboxMenuItem();
 
-  protected CheckboxMenuItem conservationMenuItem = new CheckboxMenuItem();
+  MenuItem modifyConservation = new MenuItem();
+
+  MenuItem noColourmenuItem = new MenuItem();
 
   final AlignmentPanel ap;
 
@@ -75,8 +127,6 @@ public class APopupMenu extends java.awt.PopupMenu implements
 
   MenuItem createGroupMenuItem = new MenuItem();
 
-  MenuItem nucleotideMenuItem = new MenuItem();
-
   Menu colourMenu = new Menu();
 
   CheckboxMenuItem showBoxes = new CheckboxMenuItem();
@@ -87,12 +137,29 @@ public class APopupMenu extends java.awt.PopupMenu implements
 
   CheckboxMenuItem displayNonconserved = new CheckboxMenuItem();
 
+  Menu seqShowAnnotationsMenu = new Menu(
+          MessageManager.getString("label.show_annotations"));
+
+  Menu seqHideAnnotationsMenu = new Menu(
+          MessageManager.getString("label.hide_annotations"));
+
+  MenuItem seqAddReferenceAnnotations = new MenuItem(
+          MessageManager.getString("label.add_reference_annotations"));
+
+  Menu groupShowAnnotationsMenu = new Menu(
+          MessageManager.getString("label.show_annotations"));
+
+  Menu groupHideAnnotationsMenu = new Menu(
+          MessageManager.getString("label.hide_annotations"));
+
+  MenuItem groupAddReferenceAnnotations = new MenuItem(
+          MessageManager.getString("label.add_reference_annotations"));
+
   Menu editMenu = new Menu(MessageManager.getString("action.edit"));
 
-  MenuItem copy = new MenuItem(
-          MessageManager.getString("label.jalview_copy"));
+  MenuItem copy = new MenuItem(MessageManager.getString("action.copy"));
 
-  MenuItem cut = new MenuItem(MessageManager.getString("label.jalview_cut"));
+  MenuItem cut = new MenuItem(MessageManager.getString("action.cut"));
 
   MenuItem toUpper = new MenuItem(
           MessageManager.getString("label.to_upper_case"));
@@ -123,12 +190,14 @@ public class APopupMenu extends java.awt.PopupMenu implements
           MessageManager.getString("label.edit_sequence"));
 
   MenuItem sequenceDetails = new MenuItem(
-          MessageManager.getString("label.sequence_details") + "...");
+          MessageManager.getString("label.sequence_details"));
 
   MenuItem selSeqDetails = new MenuItem(
-          MessageManager.getString("label.sequence_details") + "...");
+          MessageManager.getString("label.sequence_details"));
+
+  MenuItem makeReferenceSeq = new MenuItem();
 
-  Sequence seq;
+  SequenceI seq;
 
   MenuItem revealAll = new MenuItem();
 
@@ -141,7 +210,8 @@ public class APopupMenu extends java.awt.PopupMenu implements
 
   Menu menu1 = new Menu();
 
-  public APopupMenu(AlignmentPanel apanel, final Sequence seq, Vector links)
+  public APopupMenu(AlignmentPanel apanel, final SequenceI seq,
+          List<String> links)
   {
     // /////////////////////////////////////////////////////////
     // If this is activated from the sequence panel, the user may want to
@@ -161,21 +231,39 @@ public class APopupMenu extends java.awt.PopupMenu implements
       e.printStackTrace();
     }
 
-    for (int i = 0; i < jalview.io.AppletFormatAdapter.WRITEABLE_FORMATS.length; i++)
+    for (String ff : FileFormats.getInstance().getWritableFormats(true))
     {
-      MenuItem item = new MenuItem(
-              jalview.io.AppletFormatAdapter.WRITEABLE_FORMATS[i]);
+      MenuItem item = new MenuItem(ff);
 
       item.addActionListener(this);
       outputmenu.add(item);
     }
 
-    SequenceGroup sg = ap.av.getSelectionGroup();
+    buildAnnotationSubmenus();
 
+    SequenceGroup sg = ap.av.getSelectionGroup();
     if (sg != null && sg.getSize() > 0)
     {
-      editGroupName.setLabel(MessageManager.formatMessage(
-              "label.name_param", new String[]
+      if (sg.isNucleotide())
+      {
+        conservationColour.setEnabled(false);
+        clustalColour.setEnabled(false);
+        BLOSUM62Colour.setEnabled(false);
+        zappoColour.setEnabled(false);
+        taylorColour.setEnabled(false);
+        hydrophobicityColour.setEnabled(false);
+        helixColour.setEnabled(false);
+        strandColour.setEnabled(false);
+        turnColour.setEnabled(false);
+        buriedColour.setEnabled(false);
+      }
+      else
+      {
+        purinePyrimidineColour.setEnabled(false);
+        nucleotideColour.setEnabled(false);
+      }
+      editGroupName.setLabel(
+              MessageManager.formatMessage("label.name_param", new Object[]
               { sg.getName() }));
       showText.setState(sg.getDisplayText());
       showColourText.setState(sg.getColourText());
@@ -190,8 +278,15 @@ public class APopupMenu extends java.awt.PopupMenu implements
       {
         menu1.setLabel(MessageManager.getString("action.edit_group"));
         groupMenu.remove(createGroupMenuItem);
+        if (sg.cs != null)
+        {
+          abovePIDColour.setState(sg.cs.getThreshold() > 0);
+          conservationColour.setState(sg.cs.conservationApplied());
+          modifyPID.setEnabled(abovePIDColour.getState());
+          modifyConservation.setEnabled(conservationColour.getState());
+        }
       }
-
+      setSelectedColour(sg.cs);
     }
     else
     {
@@ -201,130 +296,26 @@ public class APopupMenu extends java.awt.PopupMenu implements
 
     if (links != null && links.size() > 0)
     {
-      Menu linkMenu = new Menu(MessageManager.getString("action.link"));
-      String link;
-      for (int i = 0; i < links.size(); i++)
-      {
-        link = links.elementAt(i).toString();
-        UrlLink urlLink = new UrlLink(link);
-        if (!urlLink.isValid())
-        {
-          System.err.println(urlLink.getInvalidMessage());
-          continue;
-        }
-        final String target = urlLink.getTarget(); // link.substring(0,
-        // link.indexOf("|"));
-        final String label = urlLink.getLabel();
-        if (seq != null && urlLink.isDynamic())
-        {
-
-          // collect matching db-refs
-          DBRefEntry[] dbr = jalview.util.DBRefUtils.selectRefs(
-                  seq.getDBRef(), new String[]
-                  { target });
-          // collect id string too
-          String id = seq.getName();
-          String descr = seq.getDescription();
-          if (descr != null && descr.length() < 1)
-          {
-            descr = null;
-          }
-          if (dbr != null)
-          {
-            for (int r = 0; r < dbr.length; r++)
-            {
-              if (id != null && dbr[r].getAccessionId().equals(id))
-              {
-                // suppress duplicate link creation for the bare sequence ID
-                // string with this link
-                id = null;
-              }
-              // create Bare ID link for this RUL
-              String[] urls = urlLink.makeUrls(dbr[r].getAccessionId(),
-                      true);
-              if (urls != null)
-              {
-                for (int u = 0; u < urls.length; u += 2)
-                {
-                  addshowLink(linkMenu, label + "|" + urls[u], urls[u + 1]);
-                }
-              }
-            }
-          }
-          if (id != null)
-          {
-            // create Bare ID link for this RUL
-            String[] urls = urlLink.makeUrls(id, true);
-            if (urls != null)
-            {
-              for (int u = 0; u < urls.length; u += 2)
-              {
-                addshowLink(linkMenu, label, urls[u + 1]);
-              }
-            }
-            // addshowLink(linkMenu, target, url_pref + id + url_suff);
-          }
-          // Now construct URLs from description but only try to do it for regex
-          // URL links
-          if (descr != null && urlLink.getRegexReplace() != null)
-          {
-            // create link for this URL from description only if regex matches
-            String[] urls = urlLink.makeUrls(descr, true);
-            if (urls != null)
-            {
-              for (int u = 0; u < urls.length; u += 2)
-              {
-                addshowLink(linkMenu, label, urls[u + 1]);
-              }
-            }
-          }
-        }
-        else
-        {
-          addshowLink(linkMenu, target, urlLink.getUrl_prefix()); // link.substring(link.lastIndexOf("|")+1));
-        }
-        /*
-         * final String url;
-         * 
-         * if (link.indexOf("$SEQUENCE_ID$") > -1) { // Substitute SEQUENCE_ID
-         * string and any matching database reference accessions String url_pref
-         * = link.substring(link.indexOf("|") + 1,
-         * link.indexOf("$SEQUENCE_ID$"));
-         * 
-         * String url_suff = link.substring(link.indexOf("$SEQUENCE_ID$") + 13);
-         * // collect matching db-refs DBRefEntry[] dbr =
-         * jalview.util.DBRefUtils.selectRefs(seq.getDBRef(), new
-         * String[]{target}); // collect id string too String id =
-         * seq.getName(); if (id.indexOf("|") > -1) { id =
-         * id.substring(id.lastIndexOf("|") + 1); } if (dbr!=null) { for (int
-         * r=0;r<dbr.length; r++) { if (dbr[r].getAccessionId().equals(id)) { //
-         * suppress duplicate link creation for the bare sequence ID string with
-         * this link id = null; } addshowLink(linkMenu,
-         * dbr[r].getSource()+"|"+dbr[r].getAccessionId(), target,
-         * url_pref+dbr[r].getAccessionId()+url_suff); } } if (id!=null) { //
-         * create Bare ID link for this RUL addshowLink(linkMenu, target,
-         * url_pref + id + url_suff); } } else { addshowLink(linkMenu, target,
-         * link.substring(link.lastIndexOf("|")+1)); }
-         */
-      }
-      if (linkMenu.getItemCount() > 0)
-      {
-        if (seq != null)
-        {
-          seqMenu.add(linkMenu);
-        }
-        else
-        {
-          add(linkMenu);
-        }
-      }
+      addFeatureLinks(seq, links);
     }
+
     // TODO: add group link menu entry here
     if (seq != null)
     {
       seqMenu.setLabel(seq.getName());
-      repGroup.setLabel(MessageManager.formatMessage(
-              "label.represent_group_with", new String[]
+      if (seq == ap.av.getAlignment().getSeqrep())
+      {
+        makeReferenceSeq.setLabel(
+                MessageManager.getString("action.unmark_as_reference"));// Unmark
+                                                                        // representative");
+      }
+      else
+      {
+        makeReferenceSeq.setLabel(
+                MessageManager.getString("action.set_as_reference")); // );
+      }
+      repGroup.setLabel(MessageManager
+              .formatMessage("label.represent_group_with", new Object[]
               { seq.getName() }));
     }
     else
@@ -354,6 +345,191 @@ public class APopupMenu extends java.awt.PopupMenu implements
   }
 
   /**
+   * Select the menu item (if any) matching the current colour scheme. This
+   * works by matching the menu item name (not display text) to the canonical
+   * name of the colour scheme.
+   * 
+   * @param cs
+   */
+  protected void setSelectedColour(ResidueShaderI cs)
+  {
+    if (cs == null || cs.getColourScheme() == null)
+    {
+      noColour.setState(true);
+    }
+    else
+    {
+      String name = cs.getColourScheme().getSchemeName();
+      for (int i = 0; i < colourMenu.getItemCount(); i++)
+      {
+        MenuItem item = colourMenu.getItem(i);
+        if (item instanceof CheckboxMenuItem)
+        {
+          if (name.equals(item.getName()))
+          {
+            ((CheckboxMenuItem) item).setState(true);
+          }
+        }
+      }
+    }
+  }
+
+  /**
+   * Adds a 'Link' menu item with a sub-menu item for each hyperlink provided.
+   * 
+   * @param seq
+   * @param links
+   */
+  void addFeatureLinks(final SequenceI seq, List<String> links)
+  {
+    Menu linkMenu = new Menu(MessageManager.getString("action.link"));
+    Map<String, List<String>> linkset = new LinkedHashMap<>();
+
+    for (String link : links)
+    {
+      UrlLink urlLink = null;
+      try
+      {
+        urlLink = new UrlLink(link);
+      } catch (Exception foo)
+      {
+        System.err.println("Exception for URLLink '" + link + "': "
+                + foo.getMessage());
+        continue;
+      }
+
+      if (!urlLink.isValid())
+      {
+        System.err.println(urlLink.getInvalidMessage());
+        continue;
+      }
+
+      urlLink.createLinksFromSeq(seq, linkset);
+    }
+
+    addshowLinks(linkMenu, linkset.values());
+
+    // disable link menu if there are no valid entries
+    if (linkMenu.getItemCount() > 0)
+    {
+      linkMenu.setEnabled(true);
+    }
+    else
+    {
+      linkMenu.setEnabled(false);
+    }
+
+    if (seq != null)
+    {
+      seqMenu.add(linkMenu);
+    }
+    else
+    {
+      add(linkMenu);
+    }
+
+  }
+
+  private void addshowLinks(Menu linkMenu, Collection<List<String>> linkset)
+  {
+    for (List<String> linkstrset : linkset)
+    {
+      // split linkstr into label and url
+      addshowLink(linkMenu, linkstrset.get(1), linkstrset.get(3));
+    }
+  }
+
+  /**
+   * Build menus for annotation types that may be shown or hidden, and for
+   * 'reference annotations' that may be added to the alignment.
+   */
+  private void buildAnnotationSubmenus()
+  {
+    /*
+     * First for the currently selected sequence (if there is one):
+     */
+    final List<SequenceI> selectedSequence = (seq == null
+            ? Collections.<SequenceI> emptyList()
+            : Arrays.asList(seq));
+    buildAnnotationTypesMenus(seqShowAnnotationsMenu,
+            seqHideAnnotationsMenu, selectedSequence);
+    configureReferenceAnnotationsMenu(seqAddReferenceAnnotations,
+            selectedSequence);
+
+    /*
+     * and repeat for the current selection group (if there is one):
+     */
+    final List<SequenceI> selectedGroup = (ap.av.getSelectionGroup() == null
+            ? Collections.<SequenceI> emptyList()
+            : ap.av.getSelectionGroup().getSequences());
+    buildAnnotationTypesMenus(groupShowAnnotationsMenu,
+            groupHideAnnotationsMenu, selectedGroup);
+    configureReferenceAnnotationsMenu(groupAddReferenceAnnotations,
+            selectedGroup);
+  }
+
+  /**
+   * Determine whether or not to enable 'add reference annotations' menu item.
+   * It is enable if there are any annotations, on any of the selected
+   * sequences, which are not yet on the alignment (visible or not).
+   * 
+   * @param menu
+   * @param forSequences
+   */
+  private void configureReferenceAnnotationsMenu(MenuItem menuItem,
+          List<SequenceI> forSequences)
+  {
+    menuItem.setEnabled(false);
+
+    /*
+     * Temporary store to hold distinct calcId / type pairs for the tooltip.
+     * Using TreeMap means calcIds are shown in alphabetical order.
+     */
+    SortedMap<String, String> tipEntries = new TreeMap<>();
+    final Map<SequenceI, List<AlignmentAnnotation>> candidates = new LinkedHashMap<>();
+    AlignmentI al = this.ap.av.getAlignment();
+    AlignmentUtils.findAddableReferenceAnnotations(forSequences, tipEntries,
+            candidates, al);
+    if (!candidates.isEmpty())
+    {
+      StringBuilder tooltip = new StringBuilder(64);
+      tooltip.append(MessageManager.getString("label.add_annotations_for"));
+
+      /*
+       * Found annotations that could be added. Enable the menu item, and
+       * configure its action.
+       */
+      menuItem.setEnabled(true);
+
+      menuItem.addActionListener(new ActionListener()
+      {
+        @Override
+        public void actionPerformed(ActionEvent e)
+        {
+          addReferenceAnnotations_actionPerformed(candidates);
+        }
+      });
+    }
+  }
+
+  /**
+   * Add annotations to the sequences and to the alignment.
+   * 
+   * @param candidates
+   *          a map whose keys are sequences on the alignment, and values a list
+   *          of annotations to add to each sequence
+   */
+  protected void addReferenceAnnotations_actionPerformed(
+          Map<SequenceI, List<AlignmentAnnotation>> candidates)
+  {
+    final SequenceGroup selectionGroup = this.ap.av.getSelectionGroup();
+    final AlignmentI alignment = this.ap.getAlignment();
+    AlignmentUtils.addReferenceAnnotations(candidates, alignment,
+            selectionGroup);
+    refresh();
+  }
+
+  /**
    * add a show URL menu item to the given linkMenu
    * 
    * @param linkMenu
@@ -385,6 +561,7 @@ public class APopupMenu extends java.awt.PopupMenu implements
     MenuItem item = new MenuItem(label);
     item.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         ap.alignFrame.showURL(url, target);
@@ -393,36 +570,28 @@ public class APopupMenu extends java.awt.PopupMenu implements
     linkMenu.add(item);
   }
 
+  /**
+   * Actions on selecting / unselecting a checkbox menu item
+   */
+  @Override
   public void itemStateChanged(ItemEvent evt)
   {
-    if (evt.getSource() == abovePIDColour)
-    {
-      abovePIDColour_itemStateChanged();
-    }
-    else if (evt.getSource() == showColourText)
-    {
-      showColourText_itemStateChanged();
-    }
-    else if (evt.getSource() == showText)
+    Object source = evt.getSource();
+    if (source == noColour)
     {
-      showText_itemStateChanged();
+      noColourmenuItem_actionPerformed();
     }
-    else if (evt.getSource() == showBoxes)
+    else if (source == clustalColour)
     {
-      showBoxes_itemStateChanged();
+      clustalColour_actionPerformed();
     }
-    else if (evt.getSource() == displayNonconserved)
+    else if (source == BLOSUM62Colour)
     {
-      this.showNonconserved_itemStateChanged();
+      BLOSUM62Colour_actionPerformed();
     }
-  }
-
-  public void actionPerformed(ActionEvent evt)
-  {
-    Object source = evt.getSource();
-    if (source == clustalColour)
+    else if (evt.getSource() == PIDColour)
     {
-      clustalColour_actionPerformed();
+      PIDColour_actionPerformed();
     }
     else if (source == zappoColour)
     {
@@ -452,31 +621,59 @@ public class APopupMenu extends java.awt.PopupMenu implements
     {
       buriedColour_actionPerformed();
     }
-    else if (source == nucleotideMenuItem)
+    else if (source == nucleotideColour)
     {
       nucleotideMenuItem_actionPerformed();
     }
-
-    else if (source == userDefinedColour)
+    else if (source == purinePyrimidineColour)
     {
-      userDefinedColour_actionPerformed();
+      purinePyrimidineColour_actionPerformed();
     }
-    else if (source == PIDColour)
+    else if (source == abovePIDColour)
     {
-      PIDColour_actionPerformed();
+      abovePIDColour_itemStateChanged();
     }
-    else if (source == BLOSUM62Colour)
+    else if (source == conservationColour)
     {
-      BLOSUM62Colour_actionPerformed();
+      conservationMenuItem_itemStateChanged();
     }
-    else if (source == noColourmenuItem)
+    else if (source == showColourText)
     {
-      noColourmenuItem_actionPerformed();
+      showColourText_itemStateChanged();
     }
-    else if (source == conservationMenuItem)
+    else if (source == showText)
+    {
+      showText_itemStateChanged();
+    }
+    else if (source == showBoxes)
+    {
+      showBoxes_itemStateChanged();
+    }
+    else if (source == displayNonconserved)
+    {
+      this.showNonconserved_itemStateChanged();
+    }
+  }
+
+  /**
+   * Actions on clicking a menu item
+   */
+  @Override
+  public void actionPerformed(ActionEvent evt)
+  {
+    Object source = evt.getSource();
+    if (source == userDefinedColour)
+    {
+      userDefinedColour_actionPerformed();
+    }
+    else if (source == modifyConservation)
     {
       conservationMenuItem_itemStateChanged();
     }
+    else if (source == modifyPID)
+    {
+      abovePIDColour_itemStateChanged();
+    }
     else if (source == unGroupMenuItem)
     {
       unGroupMenuItem_actionPerformed();
@@ -491,6 +688,10 @@ public class APopupMenu extends java.awt.PopupMenu implements
     {
       editName();
     }
+    else if (source == makeReferenceSeq)
+    {
+      makeReferenceSeq_actionPerformed();
+    }
     else if (source == sequenceDetails)
     {
       showSequenceDetails();
@@ -549,37 +750,40 @@ public class APopupMenu extends java.awt.PopupMenu implements
       if (sg != null)
       {
         if (seq == null)
-          seq = (Sequence) sg.getSequenceAt(0);
+        {
+          seq = sg.getSequenceAt(0);
+        }
 
-        EditNameDialog dialog = new EditNameDialog(seq.getSequenceAsString(
-                sg.getStartRes(), sg.getEndRes() + 1), null,
-                "Edit Sequence ", null,
+        EditNameDialog dialog = new EditNameDialog(
+                seq.getSequenceAsString(sg.getStartRes(),
+                        sg.getEndRes() + 1),
+                null, "Edit Sequence ", null,
 
                 ap.alignFrame, "Edit Sequence", 500, 100, true);
 
         if (dialog.accept)
         {
-          EditCommand editCommand = new EditCommand(MessageManager.getString("label.edit_sequences"),
-                  EditCommand.REPLACE, dialog.getName().replace(' ',
-                          ap.av.getGapCharacter()),
+          EditCommand editCommand = new EditCommand(
+                  MessageManager.getString("label.edit_sequences"),
+                  Action.REPLACE,
+                  dialog.getName().replace(' ', ap.av.getGapCharacter()),
                   sg.getSequencesAsArray(ap.av.getHiddenRepSequences()),
                   sg.getStartRes(), sg.getEndRes() + 1,
                   ap.av.getAlignment());
 
           ap.alignFrame.addHistoryItem(editCommand);
 
-          ap.av.firePropertyChange("alignment", null, ap.av.getAlignment()
-                  .getSequences());
+          ap.av.firePropertyChange("alignment", null,
+                  ap.av.getAlignment().getSequences());
         }
       }
     }
     else if (source == toUpper || source == toLower || source == toggleCase)
     {
       SequenceGroup sg = ap.av.getSelectionGroup();
-      Vector regions = new Vector();
       if (sg != null)
       {
-        int[][] startEnd = ap.av.getVisibleRegionBoundaries(
+        List<int[]> startEnd = ap.av.getVisibleRegionBoundaries(
                 sg.getStartRes(), sg.getEndRes() + 1);
 
         String description;
@@ -607,8 +811,8 @@ public class APopupMenu extends java.awt.PopupMenu implements
 
         ap.alignFrame.addHistoryItem(caseCommand);
 
-        ap.av.firePropertyChange("alignment", null, ap.av.getAlignment()
-                .getSequences());
+        ap.av.firePropertyChange("alignment", null,
+                ap.av.getAlignment().getSequences());
 
       }
     }
@@ -620,9 +824,9 @@ public class APopupMenu extends java.awt.PopupMenu implements
         return;
       }
 
-      int rsize = 0, gSize = sg.getSize();
-      SequenceI[] rseqs, seqs = new SequenceI[gSize];
-      SequenceFeature[] tfeatures, features = new SequenceFeature[gSize];
+      int gSize = sg.getSize();
+      List<SequenceI> seqs = new ArrayList<>();
+      List<SequenceFeature> features = new ArrayList<>();
 
       for (int i = 0; i < gSize; i++)
       {
@@ -630,25 +834,22 @@ public class APopupMenu extends java.awt.PopupMenu implements
         int end = sg.findEndRes(sg.getSequenceAt(i));
         if (start <= end)
         {
-          seqs[rsize] = sg.getSequenceAt(i);
-          features[rsize] = new SequenceFeature(null, null, null, start,
-                  end, "Jalview");
-          rsize++;
+          seqs.add(sg.getSequenceAt(i));
+          features.add(
+                  new SequenceFeature(null, null, start, end, "Jalview"));
         }
       }
-      rseqs = new SequenceI[rsize];
-      tfeatures = new SequenceFeature[rsize];
-      System.arraycopy(seqs, 0, rseqs, 0, rsize);
-      System.arraycopy(features, 0, tfeatures, 0, rsize);
-      features = tfeatures;
-      seqs = rseqs;
-
-      if (ap.seqPanel.seqCanvas.getFeatureRenderer().amendFeatures(seqs,
-              features, true, ap))
+
+      if (!seqs.isEmpty())
       {
-        ap.alignFrame.sequenceFeatures.setState(true);
-        ap.av.showSequenceFeatures(true);
-        ap.highlightSearchResults(null);
+        if (ap.seqPanel.seqCanvas.getFeatureRenderer().amendFeatures(seqs,
+                features, true, ap))
+        {
+          ap.alignFrame.sequenceFeatures.setState(true);
+          ap.av.setShowSequenceFeatures(true);
+          ap.av.setSearchResults(null); // clear highlighting
+          ap.repaint(); // draw new/amended features
+        }
       }
     }
     else
@@ -664,16 +865,17 @@ public class APopupMenu extends java.awt.PopupMenu implements
 
     Frame frame = new Frame();
     frame.add(cap);
-    jalview.bin.JalviewLite.addFrame(frame, MessageManager.formatMessage(
-            "label.selection_output_command", new String[]
+    JalviewLite.addFrame(frame, MessageManager
+            .formatMessage("label.selection_output_command", new Object[]
             { e.getActionCommand() }), 600, 500);
     // JBPNote: getSelectionAsNewSequence behaviour has changed - this method
     // now returns a full copy of sequence data
     // TODO consider using getSequenceSelection instead here
 
-    cap.setText(new jalview.io.AppletFormatAdapter().formatSequences(
-            e.getActionCommand(), 
-            ap.av.showJVSuffix, ap.av, true));
+    FileFormatI fileFormat = FileFormats.getInstance()
+            .forName(e.getActionCommand());
+    cap.setText(new AppletFormatAdapter().formatSequences(fileFormat,
+            ap.av.getShowJVSuffix(), ap, true));
 
   }
 
@@ -684,8 +886,7 @@ public class APopupMenu extends java.awt.PopupMenu implements
 
   protected void showSequenceDetails()
   {
-    createSequenceDetailsReport(new SequenceI[]
-    { seq });
+    createSequenceDetailsReport(new SequenceI[] { seq });
   }
 
   public void createSequenceDetailsReport(SequenceI[] sequences)
@@ -693,30 +894,25 @@ public class APopupMenu extends java.awt.PopupMenu implements
 
     CutAndPasteTransfer cap = new CutAndPasteTransfer(false, ap.alignFrame);
 
-    StringBuffer contents = new StringBuffer();
+    StringBuilder contents = new StringBuilder(128);
     for (SequenceI seq : sequences)
     {
-      contents.append(MessageManager.formatMessage(
-              "label.annotation_for_displayid", new String[]
+      contents.append(MessageManager
+              .formatMessage("label.annotation_for_displayid", new Object[]
               { seq.getDisplayId(true) }));
-      new SequenceAnnotationReport(null)
-              .createSequenceAnnotationReport(
-                      contents,
-                      seq,
-                      true,
-                      true,
-                      false,
-                      (ap.seqPanel.seqCanvas.fr != null) ? ap.seqPanel.seqCanvas.fr.minmax
-                              : null);
+      new SequenceAnnotationReport(false).createSequenceAnnotationReport(
+              contents, seq, true, true, ap.seqPanel.seqCanvas.fr);
       contents.append("</p>");
     }
     Frame frame = new Frame();
     frame.add(cap);
-    jalview.bin.JalviewLite.addFrame(frame, "Sequence Details for "
-            + (sequences.length == 1 ? sequences[0].getDisplayId(true)
-                    : "Selection"), 600, 500);
-    cap.setText(MessageManager.formatMessage("label.html_content",
-            new String[]
+    jalview.bin.JalviewLite.addFrame(frame,
+            "Sequence Details for " + (sequences.length == 1
+                    ? sequences[0].getDisplayId(true)
+                    : "Selection"),
+            600, 500);
+    cap.setText(
+            MessageManager.formatMessage("label.html_content", new Object[]
             { contents.toString() }));
   }
 
@@ -731,33 +927,39 @@ public class APopupMenu extends java.awt.PopupMenu implements
     {
       seq.setName(dialog.getName());
       seq.setDescription(dialog.getDescription());
-      ap.paintAlignment(false);
+      ap.paintAlignment(false, false);
     }
   }
 
   void addPDB()
   {
-    if (seq.getPDBId() != null)
+    Vector<PDBEntry> pdbs = seq.getAllPDBEntries();
+    if (pdbs != null && !pdbs.isEmpty())
     {
-      PDBEntry entry = (PDBEntry) seq.getPDBId().firstElement();
+      PDBEntry entry = pdbs.firstElement();
 
       if (ap.av.applet.jmolAvailable)
-        new jalview.appletgui.AppletJmol(entry, new Sequence[]
-        { seq }, null, ap, AppletFormatAdapter.URL);
+      {
+        new AppletJmol(entry, new SequenceI[] { seq }, null, ap,
+                DataSourceType.URL);
+      }
       else
-        new MCview.AppletPDBViewer(entry, new Sequence[]
-        { seq }, null, ap, AppletFormatAdapter.URL);
+      {
+        new mc_view.AppletPDBViewer(entry, new SequenceI[] { seq }, null,
+                ap, DataSourceType.URL);
+      }
 
     }
     else
     {
-      CutAndPasteTransfer cap = new CutAndPasteTransfer(true, ap.alignFrame);
+      CutAndPasteTransfer cap = new CutAndPasteTransfer(true,
+              ap.alignFrame);
       cap.setText(MessageManager.getString("label.paste_pdb_file"));
       cap.setPDBImport(seq);
       Frame frame = new Frame();
       frame.add(cap);
-      jalview.bin.JalviewLite.addFrame(frame, MessageManager.formatMessage(
-              "label.paste_pdb_file_for_sequence", new String[]
+      JalviewLite.addFrame(frame, MessageManager.formatMessage(
+              "label.paste_pdb_file_for_sequence", new Object[]
               { seq.getName() }), 400, 300);
     }
   }
@@ -768,19 +970,16 @@ public class APopupMenu extends java.awt.PopupMenu implements
     sequenceFeature.addActionListener(this);
 
     editGroupName.addActionListener(this);
-    unGroupMenuItem.setLabel(MessageManager
-            .getString("action.remove_group"));
+    unGroupMenuItem
+            .setLabel(MessageManager.getString("action.remove_group"));
     unGroupMenuItem.addActionListener(this);
 
-    createGroupMenuItem.setLabel(MessageManager
-            .getString("action.create_group"));
+    createGroupMenuItem
+            .setLabel(MessageManager.getString("action.create_group"));
     createGroupMenuItem.addActionListener(this);
 
-    nucleotideMenuItem.setLabel(MessageManager
-            .getString("label.nucleotide"));
-    nucleotideMenuItem.addActionListener(this);
-    conservationMenuItem.addItemListener(this);
-    abovePIDColour.addItemListener(this);
+    modifyPID.setEnabled(abovePIDColour.getState());
+    modifyConservation.setEnabled(conservationColour.getState());
     colourMenu.setLabel(MessageManager.getString("label.group_colour"));
     showBoxes.setLabel(MessageManager.getString("action.boxes"));
     showBoxes.setState(true);
@@ -788,8 +987,8 @@ public class APopupMenu extends java.awt.PopupMenu implements
     sequenceName.addActionListener(this);
     sequenceDetails.addActionListener(this);
     selSeqDetails.addActionListener(this);
-    displayNonconserved.setLabel(MessageManager
-            .getString("label.show_non_conversed"));
+    displayNonconserved
+            .setLabel(MessageManager.getString("label.show_non_conserved"));
     displayNonconserved.setState(false);
     displayNonconserved.addItemListener(this);
     showText.setLabel(MessageManager.getString("action.text"));
@@ -800,18 +999,21 @@ public class APopupMenu extends java.awt.PopupMenu implements
     seqMenu.setLabel(MessageManager.getString("label.sequence"));
     pdb.setLabel(MessageManager.getString("label.view_pdb_structure"));
     hideSeqs.setLabel(MessageManager.getString("action.hide_sequences"));
-    repGroup.setLabel(MessageManager.formatMessage(
-            "label.represent_group_with", new String[]
+    repGroup.setLabel(MessageManager
+            .formatMessage("label.represent_group_with", new Object[]
             { "" }));
     revealAll.setLabel(MessageManager.getString("action.reveal_all"));
     revealSeq.setLabel(MessageManager.getString("action.reveal_sequences"));
-    menu1.setLabel(MessageManager.getString("label.group") + ":");
+    menu1.setLabel(MessageManager.getString("label.group:"));
     add(groupMenu);
     this.add(seqMenu);
     this.add(hideSeqs);
     this.add(revealSeq);
     this.add(revealAll);
     // groupMenu.add(selSeqDetails);
+    groupMenu.add(groupShowAnnotationsMenu);
+    groupMenu.add(groupHideAnnotationsMenu);
+    groupMenu.add(groupAddReferenceAnnotations);
     groupMenu.add(editMenu);
     groupMenu.add(outputmenu);
     groupMenu.add(sequenceFeature);
@@ -819,7 +1021,7 @@ public class APopupMenu extends java.awt.PopupMenu implements
     groupMenu.add(unGroupMenuItem);
     groupMenu.add(menu1);
 
-    colourMenu.add(noColourmenuItem);
+    colourMenu.add(noColour);
     colourMenu.add(clustalColour);
     colourMenu.add(BLOSUM62Colour);
     colourMenu.add(PIDColour);
@@ -830,40 +1032,91 @@ public class APopupMenu extends java.awt.PopupMenu implements
     colourMenu.add(strandColour);
     colourMenu.add(turnColour);
     colourMenu.add(buriedColour);
-    colourMenu.add(nucleotideMenuItem);
+    colourMenu.add(nucleotideColour);
+    colourMenu.add(purinePyrimidineColour);
     colourMenu.add(userDefinedColour);
     colourMenu.addSeparator();
+    colourMenu.add(conservationColour);
+    colourMenu.add(modifyConservation);
     colourMenu.add(abovePIDColour);
-    colourMenu.add(conservationMenuItem);
-
-    noColourmenuItem.setLabel(MessageManager.getString("label.none"));
-    noColourmenuItem.addActionListener(this);
-
-    clustalColour.setLabel(MessageManager.getString("label.clustalx_colours"));
-    clustalColour.addActionListener(this);
-    zappoColour.setLabel(MessageManager.getString("label.zappo"));
-    zappoColour.addActionListener(this);
-    taylorColour.setLabel(MessageManager.getString("label.taylor"));
-    taylorColour.addActionListener(this);
-    hydrophobicityColour.setLabel(MessageManager.getString("label.hydrophobicity"));
-    hydrophobicityColour.addActionListener(this);
-    helixColour.setLabel(MessageManager.getString("label.helix_propensity"));
-    helixColour.addActionListener(this);
-    strandColour.setLabel(MessageManager.getString("label.strand_propensity"));
-    strandColour.addActionListener(this);
-    turnColour.setLabel(MessageManager.getString("label.turn_propensity"));
-    turnColour.addActionListener(this);
-    buriedColour.setLabel(MessageManager.getString("label.buried_index"));
-    buriedColour.addActionListener(this);
-    abovePIDColour.setLabel(MessageManager.getString("label.above_identity_percentage"));
-
-    userDefinedColour.setLabel(MessageManager.getString("action.user_defined"));
+    colourMenu.add(modifyPID);
+
+    noColour.setLabel(MessageManager.getString("label.none"));
+    noColour.addItemListener(this);
+
+    /*
+     * setName allows setSelectedColour to do its thing
+     */
+    clustalColour.setLabel(
+            MessageManager.getString("label.colourScheme_clustal"));
+    clustalColour.setName(JalviewColourScheme.Clustal.toString());
+    clustalColour.addItemListener(this);
+    BLOSUM62Colour.setLabel(
+            MessageManager.getString("label.colourScheme_blosum62"));
+    BLOSUM62Colour.setName(JalviewColourScheme.Blosum62.toString());
+    BLOSUM62Colour.addItemListener(this);
+    PIDColour.setLabel(
+            MessageManager.getString("label.colourScheme_%identity"));
+    PIDColour.setName(JalviewColourScheme.PID.toString());
+    PIDColour.addItemListener(this);
+    zappoColour
+            .setLabel(MessageManager.getString("label.colourScheme_zappo"));
+    zappoColour.setName(JalviewColourScheme.Zappo.toString());
+    zappoColour.addItemListener(this);
+    taylorColour.setLabel(
+            MessageManager.getString("label.colourScheme_taylor"));
+    taylorColour.setName(JalviewColourScheme.Taylor.toString());
+    taylorColour.addItemListener(this);
+    hydrophobicityColour.setLabel(
+            MessageManager.getString("label.colourScheme_hydrophobic"));
+    hydrophobicityColour
+            .setName(JalviewColourScheme.Hydrophobic.toString());
+    hydrophobicityColour.addItemListener(this);
+    helixColour.setLabel(
+            MessageManager.getString("label.colourScheme_helixpropensity"));
+    helixColour.setName(JalviewColourScheme.Helix.toString());
+    helixColour.addItemListener(this);
+    strandColour.setLabel(MessageManager
+            .getString("label.colourScheme_strandpropensity"));
+    strandColour.setName(JalviewColourScheme.Strand.toString());
+    strandColour.addItemListener(this);
+    turnColour.setLabel(
+            MessageManager.getString("label.colourScheme_turnpropensity"));
+    turnColour.setName(JalviewColourScheme.Turn.toString());
+    turnColour.addItemListener(this);
+    buriedColour.setLabel(
+            MessageManager.getString("label.colourScheme_buriedindex"));
+    buriedColour.setName(JalviewColourScheme.Buried.toString());
+    buriedColour.addItemListener(this);
+    nucleotideColour.setLabel(
+            MessageManager.getString("label.colourScheme_nucleotide"));
+    nucleotideColour.setName(JalviewColourScheme.Nucleotide.toString());
+    nucleotideColour.addItemListener(this);
+    purinePyrimidineColour.setLabel(MessageManager
+            .getString("label.colourScheme_purine/pyrimidine"));
+    purinePyrimidineColour
+            .setName(JalviewColourScheme.PurinePyrimidine.toString());
+    purinePyrimidineColour.addItemListener(this);
+
+    userDefinedColour
+            .setLabel(MessageManager.getString("action.user_defined"));
     userDefinedColour.addActionListener(this);
-    PIDColour.setLabel(MessageManager.getString("action.percentage_identity"));
+
+    abovePIDColour.setLabel(
+            MessageManager.getString("label.above_identity_threshold"));
+    abovePIDColour.addItemListener(this);
+    modifyPID.setLabel(
+            MessageManager.getString("label.modify_identity_threshold"));
+    modifyPID.addActionListener(this);
+    conservationColour
+            .setLabel(MessageManager.getString("action.by_conservation"));
+    conservationColour.addItemListener(this);
+    modifyConservation.setLabel(MessageManager
+            .getString("label.modify_conservation_threshold"));
+    modifyConservation.addActionListener(this);
+
     PIDColour.addActionListener(this);
-    BLOSUM62Colour.setLabel("BLOSUM62");
     BLOSUM62Colour.addActionListener(this);
-    conservationMenuItem.setLabel(MessageManager.getString("label.conservation"));
 
     editMenu.add(copy);
     copy.addActionListener(this);
@@ -878,7 +1131,11 @@ public class APopupMenu extends java.awt.PopupMenu implements
     editMenu.add(toLower);
     toLower.addActionListener(this);
     editMenu.add(toggleCase);
+    seqMenu.add(seqShowAnnotationsMenu);
+    seqMenu.add(seqHideAnnotationsMenu);
+    seqMenu.add(seqAddReferenceAnnotations);
     seqMenu.add(sequenceName);
+    seqMenu.add(makeReferenceSeq);
     // seqMenu.add(sequenceDetails);
 
     if (!ap.av.applet.useXtrnalSviewer)
@@ -898,65 +1155,73 @@ public class APopupMenu extends java.awt.PopupMenu implements
     repGroup.addActionListener(this);
     revealAll.addActionListener(this);
     revealSeq.addActionListener(this);
+    makeReferenceSeq.addActionListener(this);
   }
 
   void refresh()
   {
-    ap.paintAlignment(true);
+    ap.paintAlignment(true, true);
   }
 
   protected void clustalColour_actionPerformed()
   {
     SequenceGroup sg = getGroup();
-    sg.cs = new ClustalxColourScheme(sg, ap.av.getHiddenRepSequences());
+    sg.cs = new ResidueShader(
+            new ClustalxColourScheme(sg, ap.av.getHiddenRepSequences()));
     refresh();
   }
 
   protected void zappoColour_actionPerformed()
   {
-    getGroup().cs = new ZappoColourScheme();
+    getGroup().cs = new ResidueShader(new ZappoColourScheme());
     refresh();
   }
 
   protected void taylorColour_actionPerformed()
   {
-    getGroup().cs = new TaylorColourScheme();
+    getGroup().cs = new ResidueShader(new TaylorColourScheme());
     refresh();
   }
 
   protected void hydrophobicityColour_actionPerformed()
   {
-    getGroup().cs = new HydrophobicColourScheme();
+    getGroup().cs = new ResidueShader(new HydrophobicColourScheme());
     refresh();
   }
 
   protected void helixColour_actionPerformed()
   {
-    getGroup().cs = new HelixColourScheme();
+    getGroup().cs = new ResidueShader(new HelixColourScheme());
     refresh();
   }
 
   protected void strandColour_actionPerformed()
   {
-    getGroup().cs = new StrandColourScheme();
+    getGroup().cs = new ResidueShader(new StrandColourScheme());
     refresh();
   }
 
   protected void turnColour_actionPerformed()
   {
-    getGroup().cs = new TurnColourScheme();
+    getGroup().cs = new ResidueShader(new TurnColourScheme());
     refresh();
   }
 
   protected void buriedColour_actionPerformed()
   {
-    getGroup().cs = new BuriedColourScheme();
+    getGroup().cs = new ResidueShader(new BuriedColourScheme());
     refresh();
   }
 
   public void nucleotideMenuItem_actionPerformed()
   {
-    getGroup().cs = new NucleotideColourScheme();
+    getGroup().cs = new ResidueShader(new NucleotideColourScheme());
+    refresh();
+  }
+
+  public void purinePyrimidineColour_actionPerformed()
+  {
+    getGroup().cs = new ResidueShader(new PurinePyrimidineColourScheme());
     refresh();
   }
 
@@ -970,12 +1235,13 @@ public class APopupMenu extends java.awt.PopupMenu implements
 
     if (abovePIDColour.getState())
     {
-      sg.cs.setConsensus(AAFrequency.calculate(sg.getSequences(ap.av
-              .getHiddenRepSequences()), 0, ap.av.getAlignment().getWidth()));
-      int threshold = SliderPanel.setPIDSliderSource(ap, sg.cs, getGroup()
-              .getName());
+      sg.cs.setConsensus(AAFrequency.calculate(
+              sg.getSequences(ap.av.getHiddenRepSequences()), 0,
+              ap.av.getAlignment().getWidth()));
+      int threshold = SliderPanel.setPIDSliderSource(ap, sg.cs,
+              getGroup().getName());
 
-      sg.cs.setThreshold(threshold, ap.av.getIgnoreGapsConsensus());
+      sg.cs.setThreshold(threshold, ap.av.isIgnoreGapsConsensus());
 
       SliderPanel.showPIDSlider();
 
@@ -983,11 +1249,11 @@ public class APopupMenu extends java.awt.PopupMenu implements
     else
     // remove PIDColouring
     {
-      sg.cs.setThreshold(0, ap.av.getIgnoreGapsConsensus());
+      SliderPanel.hidePIDSlider();
+      sg.cs.setThreshold(0, ap.av.isIgnoreGapsConsensus());
     }
-
+    modifyPID.setEnabled(abovePIDColour.getState());
     refresh();
-
   }
 
   protected void userDefinedColour_actionPerformed()
@@ -998,9 +1264,10 @@ public class APopupMenu extends java.awt.PopupMenu implements
   protected void PIDColour_actionPerformed()
   {
     SequenceGroup sg = getGroup();
-    sg.cs = new PIDColourScheme();
-    sg.cs.setConsensus(AAFrequency.calculate(sg.getSequences(ap.av
-            .getHiddenRepSequences()), 0, ap.av.getAlignment().getWidth()));
+    sg.cs = new ResidueShader(new PIDColourScheme());
+    sg.cs.setConsensus(AAFrequency.calculate(
+            sg.getSequences(ap.av.getHiddenRepSequences()), 0,
+            ap.av.getAlignment().getWidth()));
     refresh();
   }
 
@@ -1008,10 +1275,11 @@ public class APopupMenu extends java.awt.PopupMenu implements
   {
     SequenceGroup sg = getGroup();
 
-    sg.cs = new Blosum62ColourScheme();
+    sg.cs = new ResidueShader(new Blosum62ColourScheme());
 
-    sg.cs.setConsensus(AAFrequency.calculate(sg.getSequences(ap.av
-            .getHiddenRepSequences()), 0, ap.av.getAlignment().getWidth()));
+    sg.cs.setConsensus(AAFrequency.calculate(
+            sg.getSequences(ap.av.getHiddenRepSequences()), 0,
+            ap.av.getAlignment().getWidth()));
 
     refresh();
   }
@@ -1030,22 +1298,23 @@ public class APopupMenu extends java.awt.PopupMenu implements
       return;
     }
 
-    if (conservationMenuItem.getState())
+    if (conservationColour.getState())
     {
-
-      sg.cs.setConservation(Conservation.calculateConservation("Group",
-              ResidueProperties.propHash, 3, sg.getSequences(ap.av
-                      .getHiddenRepSequences()), 0, ap.av.getAlignment()
-                      .getWidth(), false, ap.av.getConsPercGaps(), false));
+      Conservation conservation = Conservation.calculateConservation(
+              "Group", sg.getSequences(ap.av.getHiddenRepSequences()), 0,
+              ap.av.getAlignment().getWidth(), false,
+              ap.av.getConsPercGaps(), false);
+      sg.getGroupColourScheme().setConservation(conservation);
       SliderPanel.setConservationSlider(ap, sg.cs, sg.getName());
       SliderPanel.showConservationSlider();
     }
     else
     // remove ConservationColouring
     {
+      SliderPanel.hideConservationSlider();
       sg.cs.setConservation(null);
     }
-
+    modifyConservation.setEnabled(conservationColour.getState());
     refresh();
   }
 
@@ -1067,7 +1336,7 @@ public class APopupMenu extends java.awt.PopupMenu implements
     SequenceGroup sg = ap.av.getSelectionGroup();
     ap.av.getAlignment().deleteGroup(sg);
     ap.av.setSelectionGroup(null);
-    ap.paintAlignment(true);
+    ap.paintAlignment(true, true);
   }
 
   void createGroupMenuItem_actionPerformed()
@@ -1088,6 +1357,29 @@ public class APopupMenu extends java.awt.PopupMenu implements
     refresh();
   }
 
+  public void makeReferenceSeq_actionPerformed()
+  {
+    if (!ap.av.getAlignment().hasSeqrep())
+    {
+      // initialise the display flags so the user sees something happen
+      ap.av.setDisplayReferenceSeq(true);
+      ap.av.setColourByReferenceSeq(true);
+      ap.av.getAlignment().setSeqrep(seq);
+    }
+    else
+    {
+      if (ap.av.getAlignment().getSeqrep() == seq)
+      {
+        ap.av.getAlignment().setSeqrep(null);
+      }
+      else
+      {
+        ap.av.getAlignment().setSeqrep(seq);
+      }
+    }
+    refresh();
+  }
+
   public void showNonconserved_itemStateChanged()
   {
     getGroup().setShowNonconserved(this.displayNonconserved.getState());
@@ -1102,36 +1394,113 @@ public class APopupMenu extends java.awt.PopupMenu implements
 
   void hideSequences(boolean representGroup)
   {
-    SequenceGroup sg = ap.av.getSelectionGroup();
-    if (sg == null || sg.getSize() < 1)
-    {
-      ap.av.hideSequence(new SequenceI[]
-      { seq });
-      return;
-    }
-
-    ap.av.setSelectionGroup(null);
+    ap.av.hideSequences(seq, representGroup);
+  }
 
-    if (representGroup)
+  /**
+   * Add annotation types to 'Show annotations' and/or 'Hide annotations' menus.
+   * "All" is added first, followed by a separator. Then add any annotation
+   * types associated with the current selection. Separate menus are built for
+   * the selected sequence group (if any), and the selected sequence.
+   * <p>
+   * Some annotation rows are always rendered together - these can be identified
+   * by a common graphGroup property > -1. Only one of each group will be marked
+   * as visible (to avoid duplication of the display). For such groups we add a
+   * composite type name, e.g.
+   * <p>
+   * IUPredWS (Long), IUPredWS (Short)
+   * 
+   * @param seq
+   */
+  protected void buildAnnotationTypesMenus(Menu showMenu, Menu hideMenu,
+          List<SequenceI> forSequences)
+  {
+    showMenu.removeAll();
+    hideMenu.removeAll();
+
+    final List<String> all = Arrays
+            .asList(new String[]
+            { MessageManager.getString("label.all") });
+    addAnnotationTypeToShowHide(showMenu, forSequences, "", all, true,
+            true);
+    addAnnotationTypeToShowHide(hideMenu, forSequences, "", all, true,
+            false);
+    showMenu.addSeparator();
+    hideMenu.addSeparator();
+
+    final AlignmentAnnotation[] annotations = ap.getAlignment()
+            .getAlignmentAnnotation();
+
+    /*
+     * Find shown/hidden annotations types, distinguished by source (calcId),
+     * and grouped by graphGroup. Using LinkedHashMap means we will retrieve in
+     * the insertion order, which is the order of the annotations on the
+     * alignment.
+     */
+    Map<String, List<List<String>>> shownTypes = new LinkedHashMap<>();
+    Map<String, List<List<String>>> hiddenTypes = new LinkedHashMap<>();
+    AlignmentAnnotationUtils.getShownHiddenTypes(shownTypes, hiddenTypes,
+            AlignmentAnnotationUtils.asList(annotations), forSequences);
+
+    for (String calcId : hiddenTypes.keySet())
     {
-      ap.av.hideRepSequences(seq, sg);
-
-      return;
+      for (List<String> type : hiddenTypes.get(calcId))
+      {
+        addAnnotationTypeToShowHide(showMenu, forSequences, calcId, type,
+                false, true);
+      }
     }
+    // grey out 'show annotations' if none are hidden
+    showMenu.setEnabled(!hiddenTypes.isEmpty());
 
-    int gsize = sg.getSize();
-    SequenceI[] hseqs;
-
-    hseqs = new SequenceI[gsize];
-
-    int index = 0;
-    for (int i = 0; i < gsize; i++)
+    for (String calcId : shownTypes.keySet())
     {
-      hseqs[index++] = sg.getSequenceAt(i);
+      for (List<String> type : shownTypes.get(calcId))
+      {
+        addAnnotationTypeToShowHide(hideMenu, forSequences, calcId, type,
+                false, false);
+      }
     }
+    // grey out 'hide annotations' if none are shown
+    hideMenu.setEnabled(!shownTypes.isEmpty());
+  }
 
-    ap.av.hideSequence(hseqs);
-    ap.av.sendSelection();
+  /**
+   * Add one annotation type to the 'Show Annotations' or 'Hide Annotations'
+   * menus.
+   * 
+   * @param showOrHideMenu
+   *          the menu to add to
+   * @param forSequences
+   *          the sequences whose annotations may be shown or hidden
+   * @param calcId
+   * @param types
+   *          the label to add
+   * @param allTypes
+   *          if true this is a special label meaning 'All'
+   * @param actionIsShow
+   *          if true, the select menu item action is to show the annotation
+   *          type, else hide
+   */
+  protected void addAnnotationTypeToShowHide(Menu showOrHideMenu,
+          final List<SequenceI> forSequences, String calcId,
+          final List<String> types, final boolean allTypes,
+          final boolean actionIsShow)
+  {
+    String label = types.toString(); // [a, b, c]
+    label = label.substring(1, label.length() - 1);
+    final MenuItem item = new MenuItem(label);
+    item.addActionListener(new java.awt.event.ActionListener()
+    {
+      @Override
+      public void actionPerformed(ActionEvent e)
+      {
+        AlignmentUtils.showOrHideSequenceAnnotations(ap.getAlignment(),
+                types, forSequences, allTypes, actionIsShow);
+        refresh();
+      }
+    });
+    showOrHideMenu.add(item);
   }
 
 }