JAL-1645 Version-Rel Version 2.9 Year-Rel 2015 Licensing glob
[jalview.git] / src / jalview / gui / PopupMenu.java
index 0216276..d344c0c 100644 (file)
@@ -1,46 +1,85 @@
 /*
- * Jalview - A Sequence Alignment Editor and Viewer (Version 2.8)
- * Copyright (C) 2012 J Procter, AM Waterhouse, LM Lui, J Engelhardt, G Barton, M Clamp, S Searle
+ * Jalview - A Sequence Alignment Editor and Viewer (Version 2.9)
+ * Copyright (C) 2015 The Jalview Authors
  * 
  * This file is part of Jalview.
  * 
  * Jalview is free software: you can redistribute it and/or
  * modify it under the terms of the GNU General Public License 
- * as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.
+ * as published by the Free Software Foundation, either version 3
+ * of the License, or (at your option) any later version.
  *  
  * Jalview is distributed in the hope that it will be useful, but 
  * WITHOUT ANY WARRANTY; without even the implied warranty 
  * of MERCHANTABILITY or FITNESS FOR A PARTICULAR 
  * PURPOSE.  See the GNU General Public License for more details.
  * 
- * You should have received a copy of the GNU General Public License along with Jalview.  If not, see <http://www.gnu.org/licenses/>.
+ * You should have received a copy of the GNU General Public License
+ * along with Jalview.  If not, see <http://www.gnu.org/licenses/>.
+ * The Jalview Authors are detailed in the 'AUTHORS' file.
  */
 package jalview.gui;
 
-import java.util.*;
-
-import java.awt.*;
-import java.awt.event.*;
-
-import javax.swing.*;
-import javax.xml.parsers.ParserConfigurationException;
-
-import org.xml.sax.SAXException;
-
-import fr.orsay.lri.varna.exceptions.ExceptionFileFormatOrSyntax;
-import fr.orsay.lri.varna.exceptions.ExceptionLoadingFailed;
-import fr.orsay.lri.varna.exceptions.ExceptionPermissionDenied;
-import fr.orsay.lri.varna.exceptions.ExceptionUnmatchedClosingParentheses;
-
-import jalview.analysis.*;
-import jalview.commands.*;
-import jalview.datamodel.*;
-import jalview.io.*;
-import jalview.schemes.*;
+import jalview.analysis.AAFrequency;
+import jalview.analysis.AlignmentAnnotationUtils;
+import jalview.analysis.AlignmentUtils;
+import jalview.analysis.Conservation;
+import jalview.commands.ChangeCaseCommand;
+import jalview.commands.EditCommand;
+import jalview.commands.EditCommand.Action;
+import jalview.datamodel.AlignmentAnnotation;
+import jalview.datamodel.AlignmentI;
+import jalview.datamodel.Annotation;
+import jalview.datamodel.ColumnSelection;
+import jalview.datamodel.DBRefEntry;
+import jalview.datamodel.PDBEntry;
+import jalview.datamodel.Sequence;
+import jalview.datamodel.SequenceFeature;
+import jalview.datamodel.SequenceGroup;
+import jalview.datamodel.SequenceI;
+import jalview.io.FormatAdapter;
+import jalview.io.SequenceAnnotationReport;
+import jalview.schemes.AnnotationColourGradient;
+import jalview.schemes.Blosum62ColourScheme;
+import jalview.schemes.BuriedColourScheme;
+import jalview.schemes.ClustalxColourScheme;
+import jalview.schemes.HelixColourScheme;
+import jalview.schemes.HydrophobicColourScheme;
+import jalview.schemes.NucleotideColourScheme;
+import jalview.schemes.PIDColourScheme;
+import jalview.schemes.PurinePyrimidineColourScheme;
+import jalview.schemes.ResidueProperties;
+import jalview.schemes.StrandColourScheme;
+import jalview.schemes.TaylorColourScheme;
+import jalview.schemes.TurnColourScheme;
+import jalview.schemes.UserColourScheme;
+import jalview.schemes.ZappoColourScheme;
 import jalview.util.GroupUrlLink;
 import jalview.util.GroupUrlLink.UrlStringTooLongException;
+import jalview.util.MessageManager;
 import jalview.util.UrlLink;
 
+import java.awt.Color;
+import java.awt.event.ActionEvent;
+import java.awt.event.ActionListener;
+import java.util.Arrays;
+import java.util.Collections;
+import java.util.Hashtable;
+import java.util.LinkedHashMap;
+import java.util.List;
+import java.util.Map;
+import java.util.TreeMap;
+import java.util.Vector;
+
+import javax.swing.ButtonGroup;
+import javax.swing.JCheckBoxMenuItem;
+import javax.swing.JColorChooser;
+import javax.swing.JMenu;
+import javax.swing.JMenuItem;
+import javax.swing.JOptionPane;
+import javax.swing.JPopupMenu;
+import javax.swing.JRadioButtonMenuItem;
+
 /**
  * DOCUMENT ME!
  * 
@@ -49,6 +88,10 @@ import jalview.util.UrlLink;
  */
 public class PopupMenu extends JPopupMenu
 {
+  private static final String ALL_ANNOTATIONS = "All";
+
+  private static final String COMMA = ",";
+
   JMenu groupMenu = new JMenu();
 
   JMenuItem groupName = new JMenuItem();
@@ -78,10 +121,8 @@ public class PopupMenu extends JPopupMenu
   protected JRadioButtonMenuItem BLOSUM62Colour = new JRadioButtonMenuItem();
 
   protected JRadioButtonMenuItem purinePyrimidineColour = new JRadioButtonMenuItem();
-  protected JRadioButtonMenuItem RNAInteractionColour = new JRadioButtonMenuItem();
 
-  // protected JRadioButtonMenuItem covariationColour = new
-  // JRadioButtonMenuItem();
+  protected JRadioButtonMenuItem RNAInteractionColour = new JRadioButtonMenuItem();
 
   JRadioButtonMenuItem noColourmenuItem = new JRadioButtonMenuItem();
 
@@ -97,8 +138,14 @@ public class PopupMenu extends JPopupMenu
 
   JMenuItem sequenceSelDetails = new JMenuItem();
 
+  JMenuItem makeReferenceSeq = new JMenuItem();
+
+  JMenuItem chooseAnnotations = new JMenuItem();
+
   SequenceI sequence;
+
   JMenuItem createGroupMenuItem = new JMenuItem();
+
   JMenuItem unGroupMenuItem = new JMenuItem();
 
   JMenuItem outline = new JMenuItem();
@@ -137,23 +184,36 @@ public class PopupMenu extends JPopupMenu
 
   JMenu outputMenu = new JMenu();
 
+  JMenu seqShowAnnotationsMenu = new JMenu();
+
+  JMenu seqHideAnnotationsMenu = new JMenu();
+
+  JMenuItem seqAddReferenceAnnotations = new JMenuItem(
+          MessageManager.getString("label.add_reference_annotations"));
+
+  JMenu groupShowAnnotationsMenu = new JMenu();
+
+  JMenu groupHideAnnotationsMenu = new JMenu();
+
+  JMenuItem groupAddReferenceAnnotations = new JMenuItem(
+          MessageManager.getString("label.add_reference_annotations"));
+
   JMenuItem sequenceFeature = new JMenuItem();
 
   JMenuItem textColour = new JMenuItem();
 
   JMenu jMenu1 = new JMenu();
 
-  JMenu structureMenu = new JMenu();
+  JMenuItem pdbStructureDialog = new JMenuItem();
 
-  JMenu viewStructureMenu = new JMenu();
+  JMenu rnaStructureMenu = new JMenu();
 
-  // JMenu colStructureMenu = new JMenu();
   JMenuItem editSequence = new JMenuItem();
 
-  // JMenuItem annotationMenuItem = new JMenuItem();
-
   JMenu groupLinksMenu;
 
+  JMenuItem hideInsertions = new JMenuItem();
+
   /**
    * Creates a new PopupMenu object.
    * 
@@ -202,7 +262,6 @@ public class PopupMenu extends JPopupMenu
     colours.add(BLOSUM62Colour);
     colours.add(purinePyrimidineColour);
     colours.add(RNAInteractionColour);
-    // colours.add(covariationColour);
 
     for (int i = 0; i < jalview.io.FormatAdapter.WRITEABLE_FORMATS.length; i++)
     {
@@ -211,6 +270,7 @@ public class PopupMenu extends JPopupMenu
 
       item.addActionListener(new java.awt.event.ActionListener()
       {
+        @Override
         public void actionPerformed(ActionEvent e)
         {
           outputText_actionPerformed(e);
@@ -220,6 +280,29 @@ public class PopupMenu extends JPopupMenu
       outputMenu.add(item);
     }
 
+    /*
+     * Build menus for annotation types that may be shown or hidden, and for
+     * 'reference annotations' that may be added to the alignment. First for the
+     * currently selected sequence (if there is one):
+     */
+    final List<SequenceI> selectedSequence = (seq == null ? Collections
+            .<SequenceI> emptyList() : Arrays.asList(seq));
+    buildAnnotationTypesMenus(seqShowAnnotationsMenu,
+            seqHideAnnotationsMenu, selectedSequence);
+    configureReferenceAnnotationsMenu(seqAddReferenceAnnotations,
+            selectedSequence);
+
+    /*
+     * And repeat for the current selection group (if there is one):
+     */
+    final List<SequenceI> selectedGroup = (ap.av.getSelectionGroup() == null ? Collections
+            .<SequenceI> emptyList() : ap.av.getSelectionGroup()
+            .getSequences());
+    buildAnnotationTypesMenus(groupShowAnnotationsMenu,
+            groupHideAnnotationsMenu, selectedGroup);
+    configureReferenceAnnotationsMenu(groupAddReferenceAnnotations,
+            selectedGroup);
+
     try
     {
       jbInit();
@@ -232,122 +315,95 @@ public class PopupMenu extends JPopupMenu
     if (seq != null)
     {
       sequenceMenu.setText(sequence.getName());
-
-      if (seq.getDatasetSequence().getPDBId() != null
-              && seq.getDatasetSequence().getPDBId().size() > 0)
+      if (seq == ap.av.getAlignment().getSeqrep())
       {
-        java.util.Enumeration e = seq.getDatasetSequence().getPDBId()
-                .elements();
-
-        while (e.hasMoreElements())
-        {
-          final PDBEntry pdb = (PDBEntry) e.nextElement();
-
-          menuItem = new JMenuItem();
-          menuItem.setText(pdb.getId());
-          menuItem.addActionListener(new java.awt.event.ActionListener()
-          {
-            public void actionPerformed(ActionEvent e)
-            {
-              // TODO re JAL-860: optionally open dialog or provide a menu entry
-              // allowing user to open just one structure per sequence
-              new AppJmol(pdb, ap.av.collateForPDB(new PDBEntry[]
-              { pdb })[0], null, ap);
-              // new PDBViewer(pdb, seqs2, null, ap, AppletFormatAdapter.FILE);
-            }
-
-          });
-          viewStructureMenu.add(menuItem);
-
-          /*
-           * menuItem = new JMenuItem(); menuItem.setText(pdb.getId());
-           * menuItem.addActionListener(new java.awt.event.ActionListener() {
-           * public void actionPerformed(ActionEvent e) {
-           * colourByStructure(pdb.getId()); } });
-           * colStructureMenu.add(menuItem);
-           */
-        }
+        makeReferenceSeq.setText(MessageManager
+                .getString("action.unmark_as_reference"));
       }
       else
       {
-        if (ap.av.getAlignment().isNucleotide() == false)
-        {
-          structureMenu.remove(viewStructureMenu);
-        }
-        // structureMenu.remove(colStructureMenu);
+        makeReferenceSeq.setText(MessageManager
+                .getString("action.set_as_reference"));
       }
 
-      if (ap.av.getAlignment().isNucleotide() == true)
+      if (!ap.av.getAlignment().isNucleotide())
+      {
+        remove(rnaStructureMenu);
+      }
+      else
       {
-        AlignmentAnnotation[] aa = ap.av.getAlignment()
+        int origCount = rnaStructureMenu.getItemCount();
+        /*
+         * add menu items to 2D-render any alignment or sequence secondary
+         * structure annotation
+         */
+        AlignmentAnnotation[] aas = ap.av.getAlignment()
                 .getAlignmentAnnotation();
-        for (int i = 0; i < aa.length; i++)
+        if (aas != null)
         {
-          if (aa[i].getRNAStruc() != null)
+          for (final AlignmentAnnotation aa : aas)
           {
-            final String rnastruc = aa[i].getRNAStruc();
-            final String structureLine = aa[i].label;
-            menuItem = new JMenuItem();
-            menuItem.setText("2D RNA " + structureLine);
-            menuItem.addActionListener(new java.awt.event.ActionListener()
-            
+            if (aa.isValidStruc() && aa.sequenceRef == null)
             {
-              public void actionPerformed(ActionEvent e)
+              /*
+               * valid alignment RNA secondary structure annotation
+               */
+              menuItem = new JMenuItem();
+              menuItem.setText(MessageManager.formatMessage(
+                      "label.2d_rna_structure_line",
+                      new Object[] { aa.label }));
+              menuItem.addActionListener(new java.awt.event.ActionListener()
               {
-                       //System.out.println("1:"+structureLine);
-                       System.out.println("1:sname"+seq.getName());
-                       System.out.println("2:seq"+seq);
-                
-                       //System.out.println("3:"+seq.getSequenceAsString());
-                       System.out.println("3:strucseq"+rnastruc);
-                       //System.out.println("4:struc"+seq.getRNA());
-                       System.out.println("5:name"+seq.getName());
-                       System.out.println("6:ap"+ap);
-                       new AppVarna(structureLine, seq, seq.getSequenceAsString(), rnastruc, seq
-                            .getName(), ap);
-                       //new AppVarna(seq.getName(),seq,rnastruc,seq.getRNA(), seq.getName(), ap);
-                       System.out.println("end");
-              }
-            });
-            viewStructureMenu.add(menuItem);
+                @Override
+                public void actionPerformed(ActionEvent e)
+                {
+                  new AppVarna(seq, aa, ap);
+                }
+              });
+              rnaStructureMenu.add(menuItem);
+            }
           }
         }
 
-        // SequenceFeatures[] test = seq.getSequenceFeatures();
-
         if (seq.getAnnotation() != null)
         {
-          AlignmentAnnotation seqAnno[] = seq.getAnnotation();
-          for (int i = 0; i < seqAnno.length; i++)
+          AlignmentAnnotation seqAnns[] = seq.getAnnotation();
+          for (final AlignmentAnnotation aa : seqAnns)
           {
-            if (seqAnno[i].getRNAStruc() != null)
+            if (aa.isValidStruc())
             {
-              final String rnastruc = seqAnno[i].getRNAStruc();
-
+              /*
+               * valid sequence RNA secondary structure annotation
+               */
               // TODO: make rnastrucF a bit more nice
               menuItem = new JMenuItem();
-              menuItem.setText("2D RNA - " + seq.getName());
+              menuItem.setText(MessageManager.formatMessage(
+                      "label.2d_rna_sequence_name",
+                      new Object[] { seq.getName() }));
               menuItem.addActionListener(new java.awt.event.ActionListener()
               {
+                @Override
                 public void actionPerformed(ActionEvent e)
                 {
                   // TODO: VARNA does'nt print gaps in the sequence
-                
-                  new AppVarna(seq.getName() + " structure", seq, seq
-                          .getSequenceAsString(), rnastruc, seq.getName(),
-                          ap);
+                  new AppVarna(seq, aa, ap);
                 }
               });
-              viewStructureMenu.add(menuItem);
+              rnaStructureMenu.add(menuItem);
             }
           }
         }
-
+        if (rnaStructureMenu.getItemCount() == origCount)
+        {
+          remove(rnaStructureMenu);
+        }
       }
 
-      menuItem = new JMenuItem("Hide Sequences");
+      menuItem = new JMenuItem(
+              MessageManager.getString("action.hide_sequences"));
       menuItem.addActionListener(new java.awt.event.ActionListener()
       {
+        @Override
         public void actionPerformed(ActionEvent e)
         {
           hideSequences(false);
@@ -358,9 +414,12 @@ public class PopupMenu extends JPopupMenu
       if (ap.av.getSelectionGroup() != null
               && ap.av.getSelectionGroup().getSize() > 1)
       {
-        menuItem = new JMenuItem("Represent Group with " + seq.getName());
+        menuItem = new JMenuItem(MessageManager.formatMessage(
+                "label.represent_group_with",
+                new Object[] { seq.getName() }));
         menuItem.addActionListener(new java.awt.event.ActionListener()
         {
+          @Override
           public void actionPerformed(ActionEvent e)
           {
             hideSequences(true);
@@ -376,9 +435,11 @@ public class PopupMenu extends JPopupMenu
         if (ap.av.adjustForHiddenSeqs(index)
                 - ap.av.adjustForHiddenSeqs(index - 1) > 1)
         {
-          menuItem = new JMenuItem("Reveal Sequences");
+          menuItem = new JMenuItem(
+                  MessageManager.getString("action.reveal_sequences"));
           menuItem.addActionListener(new ActionListener()
           {
+            @Override
             public void actionPerformed(ActionEvent e)
             {
               ap.av.showSequence(index);
@@ -396,9 +457,11 @@ public class PopupMenu extends JPopupMenu
     if (ap.av.hasHiddenRows())
     {
       {
-        menuItem = new JMenuItem("Reveal All");
+        menuItem = new JMenuItem(
+                MessageManager.getString("action.reveal_all"));
         menuItem.addActionListener(new ActionListener()
         {
+          @Override
           public void actionPerformed(ActionEvent e)
           {
             ap.av.showAllHiddenSeqs();
@@ -415,12 +478,15 @@ public class PopupMenu extends JPopupMenu
     }
 
     SequenceGroup sg = ap.av.getSelectionGroup();
-    boolean isDefinedGroup = (sg!=null) ? ap.av.getAlignment().getGroups().contains(sg) : false;
+    boolean isDefinedGroup = (sg != null) ? ap.av.getAlignment()
+            .getGroups().contains(sg) : false;
 
     if (sg != null && sg.getSize() > 0)
-    {      
-      groupName.setText("Name: " + sg.getName());
-      groupName.setText("Edit name and description of current group.");
+    {
+      groupName.setText(MessageManager.formatMessage("label.name_param",
+              new Object[] { sg.getName() }));
+      groupName.setText(MessageManager
+              .getString("label.edit_name_and_description_current_group"));
 
       if (sg.cs instanceof ZappoColourScheme)
       {
@@ -470,11 +536,7 @@ public class PopupMenu extends JPopupMenu
       {
         purinePyrimidineColour.setSelected(true);
       }
-      
-      else if (sg.cs instanceof RNAInteractionColourScheme)
-      {
-        RNAInteractionColour.setSelected(true);
-      }
+
       /*
        * else if (sg.cs instanceof CovariationColourScheme) {
        * covariationColour.setSelected(true); }
@@ -498,14 +560,14 @@ public class PopupMenu extends JPopupMenu
         buildGroupURLMenu(sg, groupLinks);
       }
       // Add a 'show all structures' for the current selection
-      Hashtable<String, PDBEntry> pdbe = new Hashtable<String, PDBEntry>();
+      Hashtable<String, PDBEntry> pdbe = new Hashtable<String, PDBEntry>(), reppdb = new Hashtable<String, PDBEntry>();
       SequenceI sqass = null;
       for (SequenceI sq : ap.av.getSequenceSelection())
       {
-        Vector<PDBEntry> pes = (Vector<PDBEntry>) sq.getDatasetSequence()
-                .getPDBId();
-        if (pes != null)
+        Vector<PDBEntry> pes = sq.getDatasetSequence().getAllPDBEntries();
+        if (pes != null && pes.size() > 0)
         {
+          reppdb.put(pes.get(0).getId(), pes.get(0));
           for (PDBEntry pe : pes)
           {
             pdbe.put(pe.getId(), pe);
@@ -519,28 +581,9 @@ public class PopupMenu extends JPopupMenu
       if (pdbe.size() > 0)
       {
         final PDBEntry[] pe = pdbe.values().toArray(
-                new PDBEntry[pdbe.size()]);
-        final JMenuItem gpdbview;
-        if (pdbe.size() == 1)
-        {
-          structureMenu.add(gpdbview = new JMenuItem("View structure for "
-                  + sqass.getDisplayId(false)));
-        }
-        else
-        {
-          structureMenu.add(gpdbview = new JMenuItem("View all "
-                  + pdbe.size() + " structures."));
-        }
-        gpdbview.setToolTipText("Open a new Jmol view with all structures associated with the current selection and superimpose them using the alignment.");
-        gpdbview.addActionListener(new ActionListener()
-        {
-
-          @Override
-          public void actionPerformed(ActionEvent e)
-          {
-            new AppJmol(ap, pe, ap.av.collateForPDB(pe));
-          }
-        });
+                new PDBEntry[pdbe.size()]), pr = reppdb.values().toArray(
+                new PDBEntry[reppdb.size()]);
+        final JMenuItem gpdbview, rpdbview;
       }
     }
     else
@@ -553,23 +596,26 @@ public class PopupMenu extends JPopupMenu
     {
       createGroupMenuItem.setVisible(true);
       unGroupMenuItem.setVisible(false);
-      jMenu1.setText("Edit New Group");
-    } else {
+      jMenu1.setText(MessageManager.getString("action.edit_new_group"));
+    }
+    else
+    {
       createGroupMenuItem.setVisible(false);
       unGroupMenuItem.setVisible(true);
-      jMenu1.setText("Edit Group");
+      jMenu1.setText(MessageManager.getString("action.edit_group"));
     }
 
     if (seq == null)
     {
       sequenceMenu.setVisible(false);
-      structureMenu.setVisible(false);
+      pdbStructureDialog.setVisible(false);
+      rnaStructureMenu.setVisible(false);
     }
 
     if (links != null && links.size() > 0)
     {
 
-      JMenu linkMenu = new JMenu("Link");
+      JMenu linkMenu = new JMenu(MessageManager.getString("action.link"));
       Vector linkset = new Vector();
       for (int i = 0; i < links.size(); i++)
       {
@@ -596,8 +642,7 @@ public class PopupMenu extends JPopupMenu
 
           // collect matching db-refs
           DBRefEntry[] dbr = jalview.util.DBRefUtils.selectRefs(
-                  seq.getDBRef(), new String[]
-                  { urlLink.getTarget() });
+                  seq.getDBRef(), new String[] { urlLink.getTarget() });
           // collect id string too
           String id = seq.getName();
           String descr = seq.getDescription();
@@ -689,6 +734,133 @@ public class PopupMenu extends JPopupMenu
     }
   }
 
+  /**
+   * Add annotation types to 'Show annotations' and/or 'Hide annotations' menus.
+   * "All" is added first, followed by a separator. Then add any annotation
+   * types associated with the current selection. Separate menus are built for
+   * the selected sequence group (if any), and the selected sequence.
+   * <p>
+   * Some annotation rows are always rendered together - these can be identified
+   * by a common graphGroup property > -1. Only one of each group will be marked
+   * as visible (to avoid duplication of the display). For such groups we add a
+   * composite type name, e.g.
+   * <p>
+   * IUPredWS (Long), IUPredWS (Short)
+   * 
+   * @param seq
+   */
+  protected void buildAnnotationTypesMenus(JMenu showMenu, JMenu hideMenu,
+          List<SequenceI> forSequences)
+  {
+    showMenu.removeAll();
+    hideMenu.removeAll();
+
+    final List<String> all = Arrays.asList(ALL_ANNOTATIONS);
+    addAnnotationTypeToShowHide(showMenu, forSequences, "", all, true, true);
+    addAnnotationTypeToShowHide(hideMenu, forSequences, "", all, true,
+            false);
+    showMenu.addSeparator();
+    hideMenu.addSeparator();
+
+    final AlignmentAnnotation[] annotations = ap.getAlignment()
+            .getAlignmentAnnotation();
+
+    /*
+     * Find shown/hidden annotations types, distinguished by source (calcId),
+     * and grouped by graphGroup. Using LinkedHashMap means we will retrieve in
+     * the insertion order, which is the order of the annotations on the
+     * alignment.
+     */
+    Map<String, List<List<String>>> shownTypes = new LinkedHashMap<String, List<List<String>>>();
+    Map<String, List<List<String>>> hiddenTypes = new LinkedHashMap<String, List<List<String>>>();
+    AlignmentAnnotationUtils.getShownHiddenTypes(shownTypes, hiddenTypes,
+            AlignmentAnnotationUtils.asList(annotations), forSequences);
+
+    for (String calcId : hiddenTypes.keySet())
+    {
+      for (List<String> type : hiddenTypes.get(calcId))
+      {
+        addAnnotationTypeToShowHide(showMenu, forSequences, calcId, type,
+                false, true);
+      }
+    }
+    // grey out 'show annotations' if none are hidden
+    showMenu.setEnabled(!hiddenTypes.isEmpty());
+
+    for (String calcId : shownTypes.keySet())
+    {
+      for (List<String> type : shownTypes.get(calcId))
+      {
+        addAnnotationTypeToShowHide(hideMenu, forSequences, calcId, type,
+                false, false);
+      }
+    }
+    // grey out 'hide annotations' if none are shown
+    hideMenu.setEnabled(!shownTypes.isEmpty());
+  }
+
+  /**
+   * Returns a list of sequences - either the current selection group (if there
+   * is one), else the specified single sequence.
+   * 
+   * @param seq
+   * @return
+   */
+  protected List<SequenceI> getSequenceScope(SequenceI seq)
+  {
+    List<SequenceI> forSequences = null;
+    final SequenceGroup selectionGroup = ap.av.getSelectionGroup();
+    if (selectionGroup != null && selectionGroup.getSize() > 0)
+    {
+      forSequences = selectionGroup.getSequences();
+    }
+    else
+    {
+      forSequences = seq == null ? Collections.<SequenceI> emptyList()
+              : Arrays.asList(seq);
+    }
+    return forSequences;
+  }
+
+  /**
+   * Add one annotation type to the 'Show Annotations' or 'Hide Annotations'
+   * menus.
+   * 
+   * @param showOrHideMenu
+   *          the menu to add to
+   * @param forSequences
+   *          the sequences whose annotations may be shown or hidden
+   * @param calcId
+   * @param types
+   *          the label to add
+   * @param allTypes
+   *          if true this is a special label meaning 'All'
+   * @param actionIsShow
+   *          if true, the select menu item action is to show the annotation
+   *          type, else hide
+   */
+  protected void addAnnotationTypeToShowHide(JMenu showOrHideMenu,
+          final List<SequenceI> forSequences, String calcId,
+          final List<String> types, final boolean allTypes,
+          final boolean actionIsShow)
+  {
+    String label = types.toString(); // [a, b, c]
+    label = label.substring(1, label.length() - 1); // a, b, c
+    final JMenuItem item = new JMenuItem(label);
+    item.setToolTipText(calcId);
+    item.addActionListener(new java.awt.event.ActionListener()
+    {
+      @Override
+      public void actionPerformed(ActionEvent e)
+      {
+        AlignmentUtils.showOrHideSequenceAnnotations(ap.getAlignment(),
+                types, forSequences, allTypes, actionIsShow);
+        refresh();
+      }
+    });
+    showOrHideMenu.add(item);
+  }
+
   private void buildGroupURLMenu(SequenceGroup sg, Vector groupLinks)
   {
 
@@ -696,11 +868,18 @@ public class PopupMenu extends JPopupMenu
     // menu appears asap
     // sequence only URLs
     // ID/regex match URLs
-    groupLinksMenu = new JMenu("Group Link");
-    JMenu[] linkMenus = new JMenu[]
-    { null, new JMenu("IDS"), new JMenu("Sequences"),
-        new JMenu("IDS and Sequences") }; // three types of url that might be
-                                          // created.
+    groupLinksMenu = new JMenu(
+            MessageManager.getString("action.group_link"));
+    JMenu[] linkMenus = new JMenu[] { null,
+        new JMenu(MessageManager.getString("action.ids")),
+        new JMenu(MessageManager.getString("action.sequences")),
+        new JMenu(MessageManager.getString("action.ids_sequences")) }; // three
+                                                                       // types
+                                                                       // of url
+                                                                       // that
+                                                                       // might
+                                                                       // be
+    // created.
     SequenceI[] seqs = ap.av.getSelectionAsNewSequence();
     String[][] idandseqs = GroupUrlLink.formStrings(seqs);
     Hashtable commonDbrefs = new Hashtable();
@@ -727,8 +906,7 @@ public class PopupMenu extends JPopupMenu
           if (sarray == null)
           {
             sarray = new Object[2];
-            sarray[0] = new int[]
-            { 0 };
+            sarray[0] = new int[] { 0 };
             sarray[1] = new String[seqs.length];
 
             commonDbrefs.put(src, sarray);
@@ -809,8 +987,6 @@ public class PopupMenu extends JPopupMenu
       if (urlset != null)
       {
         int type = urlLink.getGroupURLType() & 3;
-        // System.out.println(urlLink.getGroupURLType()
-        // +" "+((String[])urlset[3])[0]);
         // first two bits ofurlLink type bitfield are sequenceids and sequences
         // TODO: FUTURE: ensure the groupURL menu structure can be generalised
         addshowLink(linkMenus[type], label
@@ -822,7 +998,8 @@ public class PopupMenu extends JPopupMenu
     }
     if (addMenu)
     {
-      groupLinksMenu = new JMenu("Group Links");
+      groupLinksMenu = new JMenu(
+              MessageManager.getString("action.group_link"));
       for (int m = 0; m < linkMenus.length; m++)
       {
         if (linkMenus[m] != null
@@ -848,14 +1025,17 @@ public class PopupMenu extends JPopupMenu
   private void addshowLink(JMenu linkMenu, String label, final String url)
   {
     JMenuItem item = new JMenuItem(label);
-    item.setToolTipText("open URL: " + url);
+    item.setToolTipText(MessageManager.formatMessage(
+            "label.open_url_param", new Object[] { url }));
     item.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         new Thread(new Runnable()
         {
 
+          @Override
           public void run()
           {
             showLink(url);
@@ -883,23 +1063,20 @@ public class PopupMenu extends JPopupMenu
           final GroupUrlLink urlgenerator, final Object[] urlstub)
   {
     JMenuItem item = new JMenuItem(label);
-    item.setToolTipText("open URL (" + urlgenerator.getUrl_prefix()
-            + "..) (" + urlgenerator.getNumberInvolved(urlstub) + " seqs)"); // TODO:
-                                                                             // put
-                                                                             // in
-                                                                             // info
-                                                                             // about
-                                                                             // what
-                                                                             // is
-                                                                             // being
-                                                                             // sent.
+    item.setToolTipText(MessageManager.formatMessage(
+            "label.open_url_seqs_param",
+            new Object[] { urlgenerator.getUrl_prefix(),
+                urlgenerator.getNumberInvolved(urlstub) }));
+    // TODO: put in info about what is being sent.
     item.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         new Thread(new Runnable()
         {
 
+          @Override
           public void run()
           {
             try
@@ -925,208 +1102,301 @@ public class PopupMenu extends JPopupMenu
    */
   private void jbInit() throws Exception
   {
-    groupMenu.setText("Group");
-    groupMenu.setText("Selection");
-    groupName.setText("Name");
+    groupMenu.setText(MessageManager.getString("label.group"));
+    groupMenu.setText(MessageManager.getString("label.selection"));
+    groupName.setText(MessageManager.getString("label.name"));
     groupName.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         groupName_actionPerformed();
       }
     });
-    sequenceMenu.setText("Sequence");
-    sequenceName.setText("Edit Name/Description");
+    sequenceMenu.setText(MessageManager.getString("label.sequence"));
+    sequenceName.setText(MessageManager
+            .getString("label.edit_name_description"));
     sequenceName.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         sequenceName_actionPerformed();
       }
     });
-    sequenceDetails.setText("Sequence Details ...");
+    chooseAnnotations.setText(MessageManager
+            .getString("action.choose_annotations"));
+    chooseAnnotations.addActionListener(new java.awt.event.ActionListener()
+    {
+      @Override
+      public void actionPerformed(ActionEvent e)
+      {
+        chooseAnnotations_actionPerformed(e);
+      }
+    });
+    sequenceDetails.setText(MessageManager
+            .getString("label.sequence_details"));
     sequenceDetails.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         sequenceDetails_actionPerformed();
       }
     });
-    sequenceSelDetails.setText("Sequence Details ...");
+    sequenceSelDetails.setText(MessageManager
+            .getString("label.sequence_details"));
     sequenceSelDetails
             .addActionListener(new java.awt.event.ActionListener()
             {
+              @Override
               public void actionPerformed(ActionEvent e)
               {
                 sequenceSelectionDetails_actionPerformed();
               }
             });
     PIDColour.setFocusPainted(false);
-    unGroupMenuItem.setText("Remove Group");
+    unGroupMenuItem
+            .setText(MessageManager.getString("action.remove_group"));
     unGroupMenuItem.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         unGroupMenuItem_actionPerformed();
       }
     });
-    createGroupMenuItem.setText("Create Group");
-    createGroupMenuItem.addActionListener(new java.awt.event.ActionListener()
-    {
-      public void actionPerformed(ActionEvent e)
-      {
-        createGroupMenuItem_actionPerformed();
-      }
-    });
+    createGroupMenuItem.setText(MessageManager
+            .getString("action.create_group"));
+    createGroupMenuItem
+            .addActionListener(new java.awt.event.ActionListener()
+            {
+              @Override
+              public void actionPerformed(ActionEvent e)
+              {
+                createGroupMenuItem_actionPerformed();
+              }
+            });
 
-    outline.setText("Border colour");
+    outline.setText(MessageManager.getString("action.border_colour"));
     outline.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         outline_actionPerformed();
       }
     });
-    nucleotideMenuItem.setText("Nucleotide");
+    nucleotideMenuItem
+            .setText(MessageManager.getString("label.nucleotide"));
     nucleotideMenuItem.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         nucleotideMenuItem_actionPerformed();
       }
     });
-    colourMenu.setText("Group Colour");
-    showBoxes.setText("Boxes");
+    colourMenu.setText(MessageManager.getString("label.group_colour"));
+    showBoxes.setText(MessageManager.getString("action.boxes"));
     showBoxes.setState(true);
     showBoxes.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         showBoxes_actionPerformed();
       }
     });
-    showText.setText("Text");
+    showText.setText(MessageManager.getString("action.text"));
     showText.setState(true);
     showText.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         showText_actionPerformed();
       }
     });
-    showColourText.setText("Colour Text");
+    showColourText.setText(MessageManager.getString("label.colour_text"));
     showColourText.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         showColourText_actionPerformed();
       }
     });
-    displayNonconserved.setText("Show Nonconserved");
+    displayNonconserved.setText(MessageManager
+            .getString("label.show_non_conversed"));
     displayNonconserved.setState(true);
     displayNonconserved.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         showNonconserved_actionPerformed();
       }
     });
-    editMenu.setText("Edit");
-    cut.setText("Cut");
+    editMenu.setText(MessageManager.getString("action.edit"));
+    cut.setText(MessageManager.getString("action.cut"));
     cut.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         cut_actionPerformed();
       }
     });
-    upperCase.setText("To Upper Case");
+    upperCase.setText(MessageManager.getString("label.to_upper_case"));
     upperCase.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         changeCase(e);
       }
     });
-    copy.setText("Copy");
+    copy.setText(MessageManager.getString("action.copy"));
     copy.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         copy_actionPerformed();
       }
     });
-    lowerCase.setText("To Lower Case");
+    lowerCase.setText(MessageManager.getString("label.to_lower_case"));
     lowerCase.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         changeCase(e);
       }
     });
-    toggle.setText("Toggle Case");
+    toggle.setText(MessageManager.getString("label.toggle_case"));
     toggle.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         changeCase(e);
       }
     });
-    pdbMenu.setText("Associate Structure with Sequence");
-    pdbFromFile.setText("From File");
+    pdbMenu.setText(MessageManager
+            .getString("label.associate_structure_with_sequence"));
+    pdbFromFile.setText(MessageManager.getString("label.from_file"));
     pdbFromFile.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         pdbFromFile_actionPerformed();
       }
     });
-    enterPDB.setText("Enter PDB Id");
+
+    enterPDB.setText(MessageManager.getString("label.enter_pdb_id"));
     enterPDB.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         enterPDB_actionPerformed();
       }
     });
-    discoverPDB.setText("Discover PDB ids");
+    discoverPDB.setText(MessageManager.getString("label.discover_pdb_ids"));
     discoverPDB.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         discoverPDB_actionPerformed();
       }
     });
-    outputMenu.setText("Output to Textbox...");
-    sequenceFeature.setText("Create Sequence Feature");
+    outputMenu.setText(MessageManager.getString("label.out_to_textbox")
+            + "...");
+    seqShowAnnotationsMenu.setText(MessageManager
+            .getString("label.show_annotations"));
+    seqHideAnnotationsMenu.setText(MessageManager
+            .getString("label.hide_annotations"));
+    groupShowAnnotationsMenu.setText(MessageManager
+            .getString("label.show_annotations"));
+    groupHideAnnotationsMenu.setText(MessageManager
+            .getString("label.hide_annotations"));
+    sequenceFeature.setText(MessageManager
+            .getString("label.create_sequence_feature"));
     sequenceFeature.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         sequenceFeature_actionPerformed();
       }
     });
-    textColour.setText("Text Colour");
+    textColour.setText(MessageManager.getString("label.text_colour"));
     textColour.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         textColour_actionPerformed();
       }
     });
-    jMenu1.setText("Group");
-    structureMenu.setText("Structure");
-    viewStructureMenu.setText("View Structure");
+    jMenu1.setText(MessageManager.getString("label.group"));
+    pdbStructureDialog.setText(MessageManager
+            .getString("label.show_pdbstruct_dialog"));
+    pdbStructureDialog.addActionListener(new ActionListener()
+    {
+      @Override
+      public void actionPerformed(ActionEvent actionEvent)
+      {
+        SequenceI[] selectedSeqs = new SequenceI[] { sequence };
+        if (ap.av.getSelectionGroup() != null)
+        {
+          selectedSeqs = ap.av.getSequenceSelection();
+        }
+        new StructureChooser(selectedSeqs, sequence, ap);
+      }
+    });
+
+    rnaStructureMenu.setText(MessageManager
+            .getString("label.view_rna_structure"));
+
     // colStructureMenu.setText("Colour By Structure");
-    editSequence.setText("Edit Sequence...");
+    editSequence.setText(MessageManager.getString("label.edit_sequence")
+            + "...");
     editSequence.addActionListener(new ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent actionEvent)
       {
         editSequence_actionPerformed(actionEvent);
       }
     });
+    makeReferenceSeq.setText(MessageManager
+            .getString("label.mark_as_representative"));
+    makeReferenceSeq.addActionListener(new ActionListener()
+    {
+
+      @Override
+      public void actionPerformed(ActionEvent actionEvent)
+      {
+        makeReferenceSeq_actionPerformed(actionEvent);
 
+      }
+    });
+    hideInsertions.setText(MessageManager
+            .getString("label.hide_insertions"));
+    hideInsertions.addActionListener(new ActionListener()
+    {
+
+      @Override
+      public void actionPerformed(ActionEvent e)
+      {
+        hideInsertions_actionPerformed(e);
+      }
+    });
     /*
      * annotationMenuItem.setText("By Annotation");
      * annotationMenuItem.addActionListener(new ActionListener() { public void
@@ -1136,7 +1406,25 @@ public class PopupMenu extends JPopupMenu
     groupMenu.add(sequenceSelDetails);
     add(groupMenu);
     add(sequenceMenu);
-    this.add(structureMenu);
+    add(rnaStructureMenu);
+    add(pdbStructureDialog);
+    if (sequence != null)
+    {
+      add(hideInsertions);
+    }
+    // annotations configuration panel suppressed for now
+    // groupMenu.add(chooseAnnotations);
+
+    /*
+     * Add show/hide annotations to the Sequence menu, and to the Selection menu
+     * (if a selection group is in force).
+     */
+    sequenceMenu.add(seqShowAnnotationsMenu);
+    sequenceMenu.add(seqHideAnnotationsMenu);
+    sequenceMenu.add(seqAddReferenceAnnotations);
+    groupMenu.add(groupShowAnnotationsMenu);
+    groupMenu.add(groupHideAnnotationsMenu);
+    groupMenu.add(groupAddReferenceAnnotations);
     groupMenu.add(editMenu);
     groupMenu.add(outputMenu);
     groupMenu.add(sequenceFeature);
@@ -1145,6 +1433,7 @@ public class PopupMenu extends JPopupMenu
     groupMenu.add(jMenu1);
     sequenceMenu.add(sequenceName);
     sequenceMenu.add(sequenceDetails);
+    sequenceMenu.add(makeReferenceSeq);
     colourMenu.add(textColour);
     colourMenu.add(noColourmenuItem);
     colourMenu.add(clustalColour);
@@ -1158,11 +1447,11 @@ public class PopupMenu extends JPopupMenu
     colourMenu.add(turnColour);
     colourMenu.add(buriedColour);
     colourMenu.add(nucleotideMenuItem);
-    if (ap.getAlignment().isNucleotide()) {
-      colourMenu.add(RNAInteractionColour);
-       colourMenu.add(purinePyrimidineColour);
+    if (ap.getAlignment().isNucleotide())
+    {
+      // JBPNote - commented since the colourscheme isn't functional
+      colourMenu.add(purinePyrimidineColour);
     }
-    // colourMenu.add(covariationColour);
     colourMenu.add(userDefinedColour);
 
     if (jalview.gui.UserDefinedColours.getUserColourSchemes() != null)
@@ -1175,6 +1464,7 @@ public class PopupMenu extends JPopupMenu
         JMenuItem item = new JMenuItem(userColours.nextElement().toString());
         item.addActionListener(new ActionListener()
         {
+          @Override
           public void actionPerformed(ActionEvent evt)
           {
             userDefinedColour_actionPerformed(evt);
@@ -1187,7 +1477,6 @@ public class PopupMenu extends JPopupMenu
     colourMenu.addSeparator();
     colourMenu.add(abovePIDColour);
     colourMenu.add(conservationMenuItem);
-    // colourMenu.add(annotationMenuItem);
     editMenu.add(copy);
     editMenu.add(cut);
     editMenu.add(editSequence);
@@ -1195,6 +1484,10 @@ public class PopupMenu extends JPopupMenu
     editMenu.add(lowerCase);
     editMenu.add(toggle);
     pdbMenu.add(pdbFromFile);
+    // JBPNote: These shouldn't be added here - should appear in a generic
+    // 'apply web service to this sequence menu'
+    // pdbMenu.add(RNAFold);
+    // pdbMenu.add(ContraFold);
     pdbMenu.add(enterPDB);
     pdbMenu.add(discoverPDB);
     jMenu1.add(groupName);
@@ -1204,144 +1497,155 @@ public class PopupMenu extends JPopupMenu
     jMenu1.add(showColourText);
     jMenu1.add(outline);
     jMenu1.add(displayNonconserved);
-    structureMenu.add(pdbMenu);
-    structureMenu.add(viewStructureMenu);
-    // structureMenu.add(colStructureMenu);
-    noColourmenuItem.setText("None");
+    noColourmenuItem.setText(MessageManager.getString("label.none"));
     noColourmenuItem.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         noColourmenuItem_actionPerformed();
       }
     });
 
-    clustalColour.setText("Clustalx colours");
+    clustalColour.setText(MessageManager
+            .getString("label.clustalx_colours"));
     clustalColour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         clustalColour_actionPerformed();
       }
     });
-    zappoColour.setText("Zappo");
+    zappoColour.setText(MessageManager.getString("label.zappo"));
     zappoColour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         zappoColour_actionPerformed();
       }
     });
-    taylorColour.setText("Taylor");
+    taylorColour.setText(MessageManager.getString("label.taylor"));
     taylorColour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         taylorColour_actionPerformed();
       }
     });
-    hydrophobicityColour.setText("Hydrophobicity");
+    hydrophobicityColour.setText(MessageManager
+            .getString("label.hydrophobicity"));
     hydrophobicityColour
             .addActionListener(new java.awt.event.ActionListener()
             {
+              @Override
               public void actionPerformed(ActionEvent e)
               {
                 hydrophobicityColour_actionPerformed();
               }
             });
-    helixColour.setText("Helix propensity");
+    helixColour.setText(MessageManager.getString("label.helix_propensity"));
     helixColour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         helixColour_actionPerformed();
       }
     });
-    strandColour.setText("Strand propensity");
+    strandColour.setText(MessageManager
+            .getString("label.strand_propensity"));
     strandColour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         strandColour_actionPerformed();
       }
     });
-    turnColour.setText("Turn propensity");
+    turnColour.setText(MessageManager.getString("label.turn_propensity"));
     turnColour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         turnColour_actionPerformed();
       }
     });
-    buriedColour.setText("Buried Index");
+    buriedColour.setText(MessageManager.getString("label.buried_index"));
     buriedColour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         buriedColour_actionPerformed();
       }
     });
-    abovePIDColour.setText("Above % Identity");
+    abovePIDColour.setText(MessageManager
+            .getString("label.above_identity_percentage"));
     abovePIDColour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         abovePIDColour_actionPerformed();
       }
     });
-    userDefinedColour.setText("User Defined...");
+    userDefinedColour.setText(MessageManager
+            .getString("action.user_defined"));
     userDefinedColour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         userDefinedColour_actionPerformed(e);
       }
     });
-    PIDColour.setText("Percentage Identity");
+    PIDColour
+            .setText(MessageManager.getString("label.percentage_identity"));
     PIDColour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         PIDColour_actionPerformed();
       }
     });
-    BLOSUM62Colour.setText("BLOSUM62");
+    BLOSUM62Colour.setText(MessageManager.getString("label.blosum62"));
     BLOSUM62Colour.addActionListener(new java.awt.event.ActionListener()
     {
+      @Override
       public void actionPerformed(ActionEvent e)
       {
         BLOSUM62Colour_actionPerformed();
       }
     });
-    purinePyrimidineColour.setText("Purine/Pyrimidine");
+    purinePyrimidineColour.setText(MessageManager
+            .getString("label.purine_pyrimidine"));
     purinePyrimidineColour
             .addActionListener(new java.awt.event.ActionListener()
             {
+              @Override
               public void actionPerformed(ActionEvent e)
               {
                 purinePyrimidineColour_actionPerformed();
               }
             });
-    
-    RNAInteractionColour.setText("RNA Interaction type");
-    RNAInteractionColour
-            .addActionListener(new java.awt.event.ActionListener()
-            {
-              public void actionPerformed(ActionEvent e)
-              {
-                RNAInteractionColour_actionPerformed();
-              }
-            });
+
     /*
      * covariationColour.addActionListener(new java.awt.event.ActionListener() {
      * public void actionPerformed(ActionEvent e) {
      * covariationColour_actionPerformed(); } });
      */
 
-    conservationMenuItem.setText("Conservation");
+    conservationMenuItem.setText(MessageManager
+            .getString("label.conservation"));
     conservationMenuItem
             .addActionListener(new java.awt.event.ActionListener()
             {
+              @Override
               public void actionPerformed(ActionEvent e)
               {
                 conservationMenuItem_actionPerformed();
@@ -1349,6 +1653,117 @@ public class PopupMenu extends JPopupMenu
             });
   }
 
+  /**
+   * Check for any annotations on the underlying dataset sequences (for the
+   * current selection group) which are not 'on the alignment'.If any are found,
+   * enable the option to add them to the alignment. The criteria for 'on the
+   * alignment' is finding an alignment annotation on the alignment, matched on
+   * calcId, label and sequenceRef.
+   * 
+   * A tooltip is also constructed that displays the source (calcId) and type
+   * (label) of the annotations that can be added.
+   * 
+   * @param menuItem
+   * @param forSequences
+   */
+  protected void configureReferenceAnnotationsMenu(JMenuItem menuItem,
+          List<SequenceI> forSequences)
+  {
+    menuItem.setEnabled(false);
+
+    /*
+     * Temporary store to hold distinct calcId / type pairs for the tooltip.
+     * Using TreeMap means calcIds are shown in alphabetical order.
+     */
+    Map<String, String> tipEntries = new TreeMap<String, String>();
+    final Map<SequenceI, List<AlignmentAnnotation>> candidates = new LinkedHashMap<SequenceI, List<AlignmentAnnotation>>();
+    AlignmentI al = this.ap.av.getAlignment();
+    AlignmentUtils.findAddableReferenceAnnotations(forSequences,
+            tipEntries, candidates, al);
+    if (!candidates.isEmpty())
+    {
+      StringBuilder tooltip = new StringBuilder(64);
+      tooltip.append(MessageManager.getString("label.add_annotations_for"));
+
+      /*
+       * Found annotations that could be added. Enable the menu item, and
+       * configure its tooltip and action.
+       */
+      menuItem.setEnabled(true);
+      for (String calcId : tipEntries.keySet())
+      {
+        tooltip.append("<br/>" + calcId + "/" + tipEntries.get(calcId));
+      }
+      String tooltipText = JvSwingUtils.wrapTooltip(true,
+              tooltip.toString());
+      menuItem.setToolTipText(tooltipText);
+
+      menuItem.addActionListener(new ActionListener()
+      {
+        @Override
+        public void actionPerformed(ActionEvent e)
+        {
+          addReferenceAnnotations_actionPerformed(candidates);
+        }
+      });
+    }
+  }
+
+  /**
+   * Add annotations to the sequences and to the alignment.
+   * 
+   * @param candidates
+   *          a map whose keys are sequences on the alignment, and values a list
+   *          of annotations to add to each sequence
+   */
+  protected void addReferenceAnnotations_actionPerformed(
+          Map<SequenceI, List<AlignmentAnnotation>> candidates)
+  {
+    final SequenceGroup selectionGroup = this.ap.av.getSelectionGroup();
+    final AlignmentI alignment = this.ap.getAlignment();
+    AlignmentUtils.addReferenceAnnotations(candidates, alignment,
+            selectionGroup);
+    refresh();
+  }
+
+  protected void makeReferenceSeq_actionPerformed(ActionEvent actionEvent)
+  {
+    if (!ap.av.getAlignment().hasSeqrep())
+    {
+      // initialise the display flags so the user sees something happen
+      ap.av.setDisplayReferenceSeq(true);
+      ap.av.setColourByReferenceSeq(true);
+      ap.av.getAlignment().setSeqrep(sequence);
+    }
+    else
+    {
+      if (ap.av.getAlignment().getSeqrep() == sequence)
+      {
+        ap.av.getAlignment().setSeqrep(null);
+      }
+      else
+      {
+        ap.av.getAlignment().setSeqrep(sequence);
+      }
+    }
+    refresh();
+  }
+
+  protected void hideInsertions_actionPerformed(ActionEvent actionEvent)
+  {
+    if (sequence != null)
+    {
+      ColumnSelection cs = ap.av.getColumnSelection();
+      if (cs == null)
+      {
+        cs = new ColumnSelection();
+      }
+      cs.hideInsertionsFor(sequence);
+      ap.av.setColumnSelection(cs);
+    }
+    refresh();
+  }
+
   protected void sequenceSelectionDetails_actionPerformed()
   {
     createSequenceDetailsReport(ap.av.getSequenceSelection());
@@ -1356,8 +1771,7 @@ public class PopupMenu extends JPopupMenu
 
   protected void sequenceDetails_actionPerformed()
   {
-    createSequenceDetailsReport(new SequenceI[]
-    { sequence });
+    createSequenceDetailsReport(new SequenceI[] { sequence });
   }
 
   public void createSequenceDetailsReport(SequenceI[] sequences)
@@ -1366,7 +1780,11 @@ public class PopupMenu extends JPopupMenu
     StringBuffer contents = new StringBuffer();
     for (SequenceI seq : sequences)
     {
-      contents.append("<p><h2>Annotation for " + seq.getDisplayId(true)
+      contents.append("<p><h2>"
+              + MessageManager
+                      .formatMessage(
+                              "label.create_sequence_details_report_annotation_for",
+                              new Object[] { seq.getDisplayId(true) })
               + "</h2></p><p>");
       new SequenceAnnotationReport(null)
               .createSequenceAnnotationReport(
@@ -1375,15 +1793,18 @@ public class PopupMenu extends JPopupMenu
                       true,
                       true,
                       false,
-                      (ap.seqPanel.seqCanvas.fr != null) ? ap.seqPanel.seqCanvas.fr.minmax
+                      (ap.getSeqPanel().seqCanvas.fr != null) ? ap
+                              .getSeqPanel().seqCanvas.fr.getMinMax()
                               : null);
       contents.append("</p>");
     }
     cap.setText("<html>" + contents.toString() + "</html>");
 
-    Desktop.instance.addInternalFrame(cap, "Sequence Details for "
-            + (sequences.length == 1 ? sequences[0].getDisplayId(true)
-                    : "Selection"), 500, 400);
+    Desktop.addInternalFrame(cap, MessageManager.formatMessage(
+            "label.sequence_details_for",
+            (sequences.length == 1 ? new Object[] { sequences[0]
+                    .getDisplayId(true) } : new Object[] { MessageManager
+                    .getString("label.selection") })), 500, 400);
 
   }
 
@@ -1519,11 +1940,6 @@ public class PopupMenu extends JPopupMenu
     refresh();
   }
 
-  protected void RNAInteractionColour_actionPerformed()
-  {
-    getGroup().cs = new RNAInteractionColourScheme();
-    refresh();
-  }
   /*
    * protected void covariationColour_actionPerformed() { getGroup().cs = new
    * CovariationColourScheme(sequence.getAnnotation()[0]); refresh(); }
@@ -1551,14 +1967,14 @@ public class PopupMenu extends JPopupMenu
       int threshold = SliderPanel.setPIDSliderSource(ap, sg.cs, getGroup()
               .getName());
 
-      sg.cs.setThreshold(threshold, ap.av.getIgnoreGapsConsensus());
+      sg.cs.setThreshold(threshold, ap.av.isIgnoreGapsConsensus());
 
       SliderPanel.showPIDSlider();
     }
     else
     // remove PIDColouring
     {
-      sg.cs.setThreshold(0, ap.av.getIgnoreGapsConsensus());
+      sg.cs.setThreshold(0, ap.av.isIgnoreGapsConsensus());
     }
 
     refresh();
@@ -1574,7 +1990,7 @@ public class PopupMenu extends JPopupMenu
   {
     SequenceGroup sg = getGroup();
 
-    if (e.getActionCommand().equals("User Defined..."))
+    if (e.getSource().equals(userDefinedColour))
     {
       new UserDefinedColours(ap, sg);
     }
@@ -1589,6 +2005,18 @@ public class PopupMenu extends JPopupMenu
   }
 
   /**
+   * Open a panel where the user can choose which types of sequence annotation
+   * to show or hide.
+   * 
+   * @param e
+   */
+  protected void chooseAnnotations_actionPerformed(ActionEvent e)
+  {
+    // todo correct way to guard against opening a duplicate panel?
+    new AnnotationChooser(ap);
+  }
+
+  /**
    * DOCUMENT ME!
    * 
    * @param e
@@ -1651,6 +2079,7 @@ public class PopupMenu extends JPopupMenu
 
     if (conservationMenuItem.isSelected())
     {
+      // JBPNote: Conservation name shouldn't be i18n translated
       Conservation c = new Conservation("Group",
               ResidueProperties.propHash, 3, sg.getSequences(ap.av
                       .getHiddenRepSequences()), sg.getStartRes(),
@@ -1685,7 +2114,7 @@ public class PopupMenu extends JPopupMenu
             sequence.getAnnotation()[0], null,
             AnnotationColourGradient.NO_THRESHOLD);
 
-    acg.predefinedColours = true;
+    acg.setPredefinedColours(true);
     sg.cs = acg;
 
     refresh();
@@ -1702,8 +2131,10 @@ public class PopupMenu extends JPopupMenu
 
     SequenceGroup sg = getGroup();
     EditNameDialog dialog = new EditNameDialog(sg.getName(),
-            sg.getDescription(), "       Group Name ",
-            "Group Description ", "Edit Group Name/Description",
+            sg.getDescription(), "       "
+                    + MessageManager.getString("label.group_name") + " ",
+            MessageManager.getString("label.group_description") + " ",
+            MessageManager.getString("label.edit_group_name_description"),
             ap.alignFrame);
 
     if (!dialog.accept)
@@ -1742,8 +2173,12 @@ public class PopupMenu extends JPopupMenu
   void sequenceName_actionPerformed()
   {
     EditNameDialog dialog = new EditNameDialog(sequence.getName(),
-            sequence.getDescription(), "       Sequence Name ",
-            "Sequence Description ", "Edit Sequence Name/Description",
+            sequence.getDescription(),
+            "       " + MessageManager.getString("label.sequence_name")
+                    + " ",
+            MessageManager.getString("label.sequence_description") + " ",
+            MessageManager
+                    .getString("label.edit_sequence_name_description"),
             ap.alignFrame);
 
     if (!dialog.accept)
@@ -1755,10 +2190,14 @@ public class PopupMenu extends JPopupMenu
     {
       if (dialog.getName().indexOf(" ") > -1)
       {
-        JOptionPane.showMessageDialog(ap,
-                "Spaces have been converted to \"_\"",
-                "No spaces allowed in Sequence Name",
-                JOptionPane.WARNING_MESSAGE);
+        JOptionPane
+                .showMessageDialog(
+                        ap,
+                        MessageManager
+                                .getString("label.spaces_converted_to_backslashes"),
+                        MessageManager
+                                .getString("label.no_spaces_allowed_sequence_name"),
+                        JOptionPane.WARNING_MESSAGE);
       }
 
       sequence.setName(dialog.getName().replace(' ', '_'));
@@ -1785,9 +2224,11 @@ public class PopupMenu extends JPopupMenu
     ap.av.setSelectionGroup(null);
     refresh();
   }
+
   void createGroupMenuItem_actionPerformed()
   {
-    getGroup(); // implicitly creates group - note - should apply defaults / use standard alignment window logic for this
+    getGroup(); // implicitly creates group - note - should apply defaults / use
+                // standard alignment window logic for this
     refresh();
   }
 
@@ -1800,7 +2241,8 @@ public class PopupMenu extends JPopupMenu
   protected void outline_actionPerformed()
   {
     SequenceGroup sg = getGroup();
-    Color col = JColorChooser.showDialog(this, "Select Outline Colour",
+    Color col = JColorChooser.showDialog(this,
+            MessageManager.getString("label.select_outline_colour"),
             Color.BLUE);
 
     if (col != null)
@@ -1854,12 +2296,10 @@ public class PopupMenu extends JPopupMenu
       jalview.util.BrowserLauncher.openURL(url);
     } catch (Exception ex)
     {
-      JOptionPane
-              .showInternalMessageDialog(
-                      Desktop.desktop,
-                      "Unixers: Couldn't find default web browser."
-                              + "\nAdd the full path to your browser in Preferences.",
-                      "Web browser not found", JOptionPane.WARNING_MESSAGE);
+      JOptionPane.showInternalMessageDialog(Desktop.desktop,
+              MessageManager.getString("label.web_browser_not_found_unix"),
+              MessageManager.getString("label.web_browser_not_found"),
+              JOptionPane.WARNING_MESSAGE);
 
       ex.printStackTrace();
     }
@@ -1870,8 +2310,7 @@ public class PopupMenu extends JPopupMenu
     SequenceGroup sg = ap.av.getSelectionGroup();
     if (sg == null || sg.getSize() < 1)
     {
-      ap.av.hideSequence(new SequenceI[]
-      { sequence });
+      ap.av.hideSequence(new SequenceI[] { sequence });
       return;
     }
 
@@ -1885,15 +2324,7 @@ public class PopupMenu extends JPopupMenu
     }
 
     int gsize = sg.getSize();
-    SequenceI[] hseqs;
-
-    hseqs = new SequenceI[gsize];
-
-    int index = 0;
-    for (int i = 0; i < gsize; i++)
-    {
-      hseqs[index++] = sg.getSequenceAt(i);
-    }
+    SequenceI[] hseqs = sg.getSequences().toArray(new SequenceI[gsize]);
 
     ap.av.hideSequence(hseqs);
     // refresh(); TODO: ? needed ?
@@ -1917,25 +2348,25 @@ public class PopupMenu extends JPopupMenu
 
     if (sg != null)
     {
-      int[][] startEnd = ap.av.getVisibleRegionBoundaries(sg.getStartRes(),
-              sg.getEndRes() + 1);
+      List<int[]> startEnd = ap.av.getVisibleRegionBoundaries(
+              sg.getStartRes(), sg.getEndRes() + 1);
 
       String description;
       int caseChange;
 
       if (source == toggle)
       {
-        description = "Toggle Case";
+        description = MessageManager.getString("label.toggle_case");
         caseChange = ChangeCaseCommand.TOGGLE_CASE;
       }
       else if (source == upperCase)
       {
-        description = "To Upper Case";
+        description = MessageManager.getString("label.to_upper_case");
         caseChange = ChangeCaseCommand.TO_UPPER;
       }
       else
       {
-        description = "To Lower Case";
+        description = MessageManager.getString("label.to_lower_case");
         caseChange = ChangeCaseCommand.TO_LOWER;
       }
 
@@ -1955,31 +2386,18 @@ public class PopupMenu extends JPopupMenu
   {
     CutAndPasteTransfer cap = new CutAndPasteTransfer();
     cap.setForInput(null);
-    Desktop.addInternalFrame(cap,
-            "Alignment output - " + e.getActionCommand(), 600, 500);
+    Desktop.addInternalFrame(cap, MessageManager.formatMessage(
+            "label.alignment_output_command",
+            new Object[] { e.getActionCommand() }), 600, 500);
 
     String[] omitHidden = null;
 
     System.out.println("PROMPT USER HERE"); // TODO: decide if a prompt happens
     // or we simply trust the user wants
     // wysiwig behaviour
-    SequenceGroup sg = ap.av.getSelectionGroup();
-    ColumnSelection csel = new ColumnSelection(ap.av.getColumnSelection());
-    omitHidden = ap.av.getViewAsString(true);
-    Alignment oal = new Alignment(ap.av.getSequenceSelection());
-    AlignmentAnnotation[] nala = ap.av.getAlignment()
-            .getAlignmentAnnotation();
-    if (nala != null)
-    {
-      for (int i = 0; i < nala.length; i++)
-      {
-        AlignmentAnnotation na = nala[i];
-        oal.addAnnotation(na);
-      }
-    }
-    cap.setText(new FormatAdapter().formatSequences(e.getActionCommand(),
-            oal, omitHidden, csel, sg));
-    oal = null;
+
+    cap.setText(new FormatAdapter(ap).formatSequences(e.getActionCommand(),
+            ap, true));
   }
 
   public void pdbFromFile_actionPerformed()
@@ -1987,10 +2405,12 @@ public class PopupMenu extends JPopupMenu
     jalview.io.JalviewFileChooser chooser = new jalview.io.JalviewFileChooser(
             jalview.bin.Cache.getProperty("LAST_DIRECTORY"));
     chooser.setFileView(new jalview.io.JalviewFileView());
-    chooser.setDialogTitle("Select a PDB file for "
-            + sequence.getDisplayId(false));
-    chooser.setToolTipText("Load a PDB file and associate it with sequence '"
-            + sequence.getDisplayId(false) + "'");
+    chooser.setDialogTitle(MessageManager.formatMessage(
+            "label.select_pdb_file_for",
+            new Object[] { sequence.getDisplayId(false) }));
+    chooser.setToolTipText(MessageManager.formatMessage(
+            "label.load_pdb_file_associate_with_sequence",
+            new Object[] { sequence.getDisplayId(false) }));
 
     int value = chooser.showOpenDialog(null);
 
@@ -1999,7 +2419,8 @@ public class PopupMenu extends JPopupMenu
       String choice = chooser.getSelectedFile().getPath();
       jalview.bin.Cache.setProperty("LAST_DIRECTORY", choice);
       new AssociatePdbFileWithSeq().associatePdbWithSeq(choice,
-              jalview.io.AppletFormatAdapter.FILE, sequence, true);
+              jalview.io.AppletFormatAdapter.FILE, sequence, true,
+              Desktop.instance);
     }
 
   }
@@ -2007,7 +2428,9 @@ public class PopupMenu extends JPopupMenu
   public void enterPDB_actionPerformed()
   {
     String id = JOptionPane.showInternalInputDialog(Desktop.desktop,
-            "Enter PDB Id", "Enter PDB Id", JOptionPane.QUESTION_MESSAGE);
+            MessageManager.getString("label.enter_pdb_id"),
+            MessageManager.getString("label.enter_pdb_id"),
+            JOptionPane.QUESTION_MESSAGE);
 
     if (id != null && id.length() > 0)
     {
@@ -2025,6 +2448,7 @@ public class PopupMenu extends JPopupMenu
             : ap.av.getSequenceSelection());
     Thread discpdb = new Thread(new Runnable()
     {
+      @Override
       public void run()
       {
 
@@ -2066,7 +2490,7 @@ public class PopupMenu extends JPopupMenu
     System.arraycopy(features, 0, tfeatures, 0, rsize);
     features = tfeatures;
     seqs = rseqs;
-    if (ap.seqPanel.seqCanvas.getFeatureRenderer().amendFeatures(seqs,
+    if (ap.getSeqPanel().seqCanvas.getFeatureRenderer().amendFeatures(seqs,
             features, true, ap))
     {
       ap.alignFrame.setShowSeqFeatures(true);
@@ -2109,17 +2533,22 @@ public class PopupMenu extends JPopupMenu
     if (sg != null)
     {
       if (sequence == null)
-        sequence = (Sequence) sg.getSequenceAt(0);
+      {
+        sequence = sg.getSequenceAt(0);
+      }
 
       EditNameDialog dialog = new EditNameDialog(
               sequence.getSequenceAsString(sg.getStartRes(),
-                      sg.getEndRes() + 1), null, "Edit Sequence ", null,
-              "Edit Sequence", ap.alignFrame);
+                      sg.getEndRes() + 1), null,
+              MessageManager.getString("label.edit_sequence"), null,
+              MessageManager.getString("label.edit_sequence"),
+              ap.alignFrame);
 
       if (dialog.accept)
       {
-        EditCommand editCommand = new EditCommand("Edit Sequences",
-                EditCommand.REPLACE, dialog.getName().replace(' ',
+        EditCommand editCommand = new EditCommand(
+                MessageManager.getString("label.edit_sequences"),
+                Action.REPLACE, dialog.getName().replace(' ',
                         ap.av.getGapCharacter()),
                 sg.getSequencesAsArray(ap.av.getHiddenRepSequences()),
                 sg.getStartRes(), sg.getEndRes() + 1, ap.av.getAlignment());