<strong>Normalise Consensus Logo</strong> to scale all columns of the
logo to the same height.
- <p>
- <strong>Group Consensus</strong><br>
- If sequence groups have been defined, then selecting option 'Group Consensus' in the <a href="../menus/alwannotation.html">Annotations menu</a> will
- result in Consensus being calculated for each group, as well as the alignment as a whole.
+ <p>
+ <strong>Group Consensus</strong><br> If sequence groups have
+ been defined, then selecting option 'Group Consensus' in the <a
+ href="../menus/alwannotation.html">Annotations menu</a> will
+ result in Consensus being calculated for each group, as well as the
+ alignment as a whole.
</p>
<p>
<strong>cDNA Consensus</strong>
<b>9</b> No. 6 (745-756)).
</ul>
<em><a
- href="http://www.compbio.dundee.ac.uk/papers/amas/amas3d.html"
- >View an HTML version of the paper</a></em>
+ href="http://www.compbio.dundee.ac.uk/papers/amas/amas3d.html">View
+ an HTML version of the paper</a></em>
</p>
<p>
Conservation is measured as a numerical index reflecting the
<strong>Colouring an alignment by conservation</strong><br>
Conservation scores can be used to colour an alignment. This is
explained further in the help page for <a
- href="../colourSchemes/conservation.html"
- >conservation colouring</a>.
+ href="../colourSchemes/conservation.html">conservation
+ colouring</a>.
</p>
<p>
- <strong>Group conservation</strong><br>
- If sequence groups have been defined, then selecting option 'Group Conservation' in the <a href="../menus/alwannotation.html">Annotations menu</a> will
- result in Conservation being calculated for each group, as well as the alignment as a whole.
+ <strong>Group conservation</strong><br> If sequence groups have
+ been defined, then selecting option 'Group Conservation' in the <a
+ href="../menus/alwannotation.html">Annotations menu</a> will
+ result in Conservation being calculated for each group, as well as
+ the alignment as a whole.
</p>
</body>
</html>
pair of sequences - computed with one of the available score
matrices, such as <a href="scorematrices.html#blosum62">BLOSUM62</a>,
<a href="scorematrices.html#pam250">PAM250</a>, or the <a
- href="scorematrices.html#simplenucleotide"
- >simple single nucleotide substitution matrix</a>. The options
- available for calculation are given in the <strong><em>Change
+ href="scorematrices.html#simplenucleotide">simple single
+ nucleotide substitution matrix</a>. The options available for
+ calculation are given in the <strong><em>Change
Parameters</em></strong> menu.
</p>
<p>
- <em>PCA Calculation modes</em><br /> The default Jalview calculation
- mode (indicated when <em><strong>Jalview PCA
- Calculation</strong></em> is ticked in the <strong><em>Change
+ <em>PCA Calculation modes</em><br /> The default Jalview
+ calculation mode (indicated when <em><strong>Jalview
+ PCA Calculation</strong></em> is ticked in the <strong><em>Change
Parameters</em></strong> menu) is to perform a PCA on a matrix where elements
in the upper diagonal give the sum of scores for mutating in one
direction, and the lower diagonal is the sum of scores for mutating
gives an asymmetric matrix, and a different PCA to a matrix produced
with the method described in the paper by G. Casari, C. Sander and
A. Valencia. Structural Biology volume 2, no. 2, February 1995 (<a
- href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7749921"
- >pubmed</a>) and implemented at the SeqSpace server at the EBI. This
- method preconditions the matrix by multiplying it with its
- transpose, and can be employed in the PCA viewer by unchecking the <strong><em>Jalview
+ href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7749921">pubmed</a>)
+ and implemented at the SeqSpace server at the EBI. This method
+ preconditions the matrix by multiplying it with its transpose, and
+ can be employed in the PCA viewer by unchecking the <strong><em>Jalview
PCA Calculation</em></strong> option in the <strong><em>Change
Parameters</em></strong> menu.
</p>
menu or pressing <strong>'CONTROL+D'</strong> brings up a dialog box
asking you to select a threshold. If the percentage identity between
the aligned positions of any two sequences in the visible alignment
- exceeds this value, the shorter sequence is discarded.<br>
- <em>Note:</em> The redundancy calculation is done when the dialog
- box is opened. For large numbers of sequences this can take a long
- time as all pairs have to be compared.
+ exceeds this value, the shorter sequence is discarded.<br> <em>Note:</em>
+ The redundancy calculation is done when the dialog box is opened.
+ For large numbers of sequences this can take a long time as all
+ pairs have to be compared.
</p>
</body>
</html>
organisms that are similar to a newly sequenced gene, or when
searching for structurally similar sequences for use in homology
modelling.</p>
-<p>
+ <p>
<strong>What happens when a reference sequence is defined ?</strong>
</p>
- <p>
- The reference sequence for an alignment is indicated by its ID being
- shown in bold. In addition:</p>
+ <p>The reference sequence for an alignment is indicated by its ID
+ being shown in bold. In addition:</p>
<ul>
<li><strong>Reference sequence numbering</strong>. Instead of
column numbers, the alignment ruler shows the reference sequence
sequence when importing analysis results, such as those returned
from <a href="../webServices/jnet.html">JPred4</a> . A reference
sequence can also be assigned via the <a
- href="../features/annotationsFormat.html#refsandviews">SET_REF</a> command in
- an alignment annotation file.</li>
+ href="../features/annotationsFormat.html#refsandviews">SET_REF</a>
+ command in an alignment annotation file.</li>
</ul>
<p>
<em>Reference sequence based alignment visualisation was
matrix, and (since 2.8.1) is available for Tree and PCA
calculations.</li>
<li><a href="#simplenucleotide">Simple Nucleotide
- Substitution</a> is a (fairly) arbitrary DNA/RNA substitution matrix.</li>
+ Substitution</a> is a (fairly) arbitrary DNA/RNA substitution
+ matrix.</li>
<!-- <li><a href="#conservation">Conservation Matrices</a> A range of matrices for distinguishing amino-acids by physicochemical property conservation.
</li> -->
</ul>
<strong><a name="pam250">PAM250</a></strong><br /> <em><strong>P</strong>ercentage
<strong>A</strong>ccepted <strong>M</strong>utation matrix. PAM250
estimates substitutions after 250% of sites have changed (each
- site can be mutated multple times).<br /> Jalview 2.8.1 introduced
- support for PAM250 based <a href="../calculations/pca.html">PCA</a>
- and <a href="../calculations/tree.html">tree</a> calculations.</em>
+ site can be mutated multple times).<br /> Jalview 2.8.1
+ introduced support for PAM250 based <a
+ href="../calculations/pca.html">PCA</a> and <a
+ href="../calculations/tree.html">tree</a> calculations.</em>
<table border="1">
<tr>
<td></td>
</table>
<strong><em>This nucleotide matrix was introduced in
Jalview 2.8. If you'd like to improve it - please take a look at <a
- href="http://issues.jalview.org/browse/JAL-1027"
- >Issue JAL-1027 - introduce a nucleotide substitution matrix that
+ href="http://issues.jalview.org/browse/JAL-1027">Issue
+ JAL-1027 - introduce a nucleotide substitution matrix that
supports RNA/DNA and ambiguity codes</a>
</em></strong>
</p>
</p>
<p>
This menu appears if the alignment contains any <a
- href="../features/annotationsFormat.html"
- >sequence associated alignment annotation</a> with associated
- score values. Each entry is the label for a distinct group of
- sequence associated annotation scores which can be used for
- sorting.
+ href="../features/annotationsFormat.html">sequence
+ associated alignment annotation</a> with associated score values.
+ Each entry is the label for a distinct group of sequence
+ associated annotation scores which can be used for sorting.
</p>
</ul>
- <p><strong>Sorting according to sequence features</strong><br/>
- Additional sort operations for
- alignments containing sequence features are provided in the
- <strong><a href="../features/featuresettings.html#sortbyfeature">Feature
+ <p>
+ <strong>Sorting according to sequence features</strong><br />
+ Additional sort operations for alignments containing sequence
+ features are provided in the <strong><a
+ href="../features/featuresettings.html#sortbyfeature">Feature
Settings</a></strong> dialog, opened via <strong>View→Feature
- Settings...</strong><p>
+ Settings...</strong>
+ <p>
<strong>Reversing the Order</strong>
</p>
<p>Selecting any item from the Sort menu will sort sequences in an
ascending order according to the property defining the sort. If the
same sort is re-applied, the sequences will be sorted in the inverse
- order. In both cases, for sequences which are equivalent under the sort
- operation, their order will be preserved (since version 2.10). In
- the case of trees and alignment orderings, Jalview will remember
+ order. In both cases, for sequences which are equivalent under the
+ sort operation, their order will be preserved (since version 2.10).
+ In the case of trees and alignment orderings, Jalview will remember
your last choice for sorting the alignment and only apply the
inverse ordering if you select the same tree or alignment ordering
item again.</p>
<strong>Alignment RNA Structure Consensus Annotation</strong>
</p>
- <p>The RNA structure consensus displayed below the alignment gives the
- percentage of valid base pairs per column for the first secondary
- structure annotation shown on the annotation panel. These values are
- shown as a histogram labeled "StrucConsensus", where a
- symbol below each bar indicates whether the majority of base pairs
- are:<ul>
+ <p>The RNA structure consensus displayed below the alignment gives
+ the percentage of valid base pairs per column for the first
+ secondary structure annotation shown on the annotation panel. These
+ values are shown as a histogram labeled "StrucConsensus",
+ where a symbol below each bar indicates whether the majority of base
+ pairs are:
+ <ul>
<li>'(' - Watson-Crick (C:G, A:U/T)</li>
<li>'[' - Non-canonical (a.ka. wobble) (G:U/T)</li>
<li>'{' - Invalid (a.k.a. tertiary) (the rest)</li>
<p>Mousing over the column gives the fraction of pairs classified
as Watson-Crick, Canonical or Invalid.</p>
- <p>By default
- this calculation includes gaps in columns. You can choose to ignore
- gaps in the calculation by right clicking on the label
- "StrucConsensus" to the left of the structure consensus bar
- chart.<br>
-
+ <p>
+ By default this calculation includes gaps in columns. You can choose
+ to ignore gaps in the calculation by right clicking on the label
+ "StrucConsensus" to the left of the structure consensus
+ bar chart.<br>
<p>
<strong>RNA Structure logo</strong><br /> Right-clicking on the
label allows you to enable the structure logo. It is very similar to
Trees are calculated on either the complete alignment, or just the
currently selected group of sequences, using the functions in the <strong>Calculate→Calculate
tree</strong> submenu. Once calculated, trees are displayed in a new <a
- href="../calculations/treeviewer.html"
- >tree viewing window</a>. There are four different calculations, using
- one of two distance measures and constructing the tree from one of
- two algorithms :
+ href="../calculations/treeviewer.html">tree viewing
+ window</a>. There are four different calculations, using one of two
+ distance measures and constructing the tree from one of two
+ algorithms :
</p>
<p>
<strong>Distance Measures</strong>
scores for the residue pairs at each aligned position.
<ul>
<li>For details about each model, see the <a
- href="scorematrices.html"
- >list of built-in score matrices</a>.
+ href="scorematrices.html">list of built-in score
+ matrices</a>.
</li>
</ul></li>
<li><strong>Sequence Feature Similarity</strong><br>Trees
</ul>
<p>
A newly calculated tree will be displayed in a new <a
- href="../calculations/treeviewer.html"
- >tree viewing window</a>. In addition, a new entry with the same tree
- viewer window name will be added in the Sort menu so that the
- alignment can be reordered to reflect the ordering of the leafs of
- the tree. If the tree was calculated on a selected region of the
- alignment, then the title of the tree view will reflect this.
+ href="../calculations/treeviewer.html">tree viewing
+ window</a>. In addition, a new entry with the same tree viewer window
+ name will be added in the Sort menu so that the alignment can be
+ reordered to reflect the ordering of the leafs of the tree. If the
+ tree was calculated on a selected region of the alignment, then the
+ title of the tree view will reflect this.
</p>
<p>
phylogenetic trees, which can cope with large numbers of sequences,
use better distance methods and can perform bootstrapping. Jalview
can read <a
- href="http://evolution.genetics.washington.edu/phylip/newick_doc.html"
- >Newick</a> format tree files using the 'Load Associated Tree' entry
- of the alignment's File menu. Sequences in the alignment will be
+ href="http://evolution.genetics.washington.edu/phylip/newick_doc.html">Newick</a>
+ format tree files using the 'Load Associated Tree' entry of the
+ alignment's File menu. Sequences in the alignment will be
automatically associated to nodes in the tree, by matching Sequence
IDs to the tree's leaf names.
</p>
</p>
<p>
The tree viewing window is opened when a tree has been <a
- href="tree.html"
- >calculated from an alignment</a>, or imported via a file or web
- service. It includes <a href="#menus">menus</a> for controlling
- layout and file and figure creation, and enables various selection
- and colouring operations on the associated sequences in the
- alignment.
+ href="tree.html">calculated from an alignment</a>, or
+ imported via a file or web service. It includes <a href="#menus">menus</a>
+ for controlling layout and file and figure creation, and enables
+ various selection and colouring operations on the associated
+ sequences in the alignment.
</p>
<p>
<strong><em>Selecting Sequence Leaf Nodes</em></strong><br>
tree is rendered and labeled:
<ul>
<li><strong>Fit to Window</strong>
- <p>The tree layout will be scaled to fit in the display window.
- You may need to reduce the font size to minimise the leaf label
- overlap when this option is selected.</p></li>
+ <p>The tree layout will be scaled to fit in the display
+ window. You may need to reduce the font size to minimise the
+ leaf label overlap when this option is selected.</p></li>
<li><strong>Font Size ...</strong><em>n</em>
- <p>
+ <p>
Brings up a dialog box to set the font size for the leaf names.
<em>n</em> is the current font size.
</p></li>
<li><strong>Show Distances</strong>
- <p>Labels each branch or leaf with its associated branch length.</p></li>
+ <p>Labels each branch or leaf with its associated branch
+ length.</p></li>
<li><strong>Show Bootstrap values</strong>
- <p>Labels each branch or leaf with its associated bootstrap
+ <p>Labels each branch or leaf with its associated bootstrap
value.</p></li>
<li><strong>Mark unlinked leaves</strong>
- <p>Toggles the display of a '*' at the beginning of a leaf label
- to indicate that there is no sequence corresponding to that leaf
- in the associated alignment.</p></li>
+ <p>Toggles the display of a '*' at the beginning of a leaf
+ label to indicate that there is no sequence corresponding to
+ that leaf in the associated alignment.</p></li>
<li><strong>Sort Alignment By Tree</strong>
<p>
Sorts any associated alignment views using the current tree. (<em>Only
<li><strong>Associate Leaves with ...</strong>
<p>
Only visible when there are <a
- href="../features/multipleViews.html"
- >multiple views</a> of the same alignment to show and edit which
- alignment views are associated with the leaves of the displayed
- tree.
+ href="../features/multipleViews.html">multiple
+ views</a> of the same alignment to show and edit which alignment
+ views are associated with the leaves of the displayed tree.
</p>
</ul>
</p>
<li>Press the "Defaults" button to reset the
minimum and maximum colours to their default settings (these
are configured in the applet's parameters or the <a
- href="../features/preferences.html"
- >application's user preferences</a>.).<br /> <em>Default min
- and max colours were introduced in Jalview 2.7</em>
+ href="../features/preferences.html">application's
+ user preferences</a>.).<br /> <em>Default min and max
+ colours were introduced in Jalview 2.7</em>
</ul>
</li>
alignment analysis (Livingstone C.D. and Barton G.J. (1993), Protein
Sequence Alignments: A Strategy for the Hierarchical Analysis of
Residue Conservation.CABIOS Vol. 9 No. 6 (745-756)). See the <a
- href="../calculations/conservation.html"
- >conservation calculation</a> help page for a more thorough
- explanation of the calculation.
+ href="../calculations/conservation.html">conservation
+ calculation</a> help page for a more thorough explanation of the
+ calculation.
</p>
<p>For an already coloured alignment, the conservation index at
each alignment position is used to modify the shading intensity of
<p>
Conservation can be calculated over all sequences in an alignment,
or just within specific groups (such as those defined by <a
- href="../calculations/tree.html"
- >phylogenetic tree partitioning</a>). The option 'apply to all groups'
- controls whether the contrast slider value will be applied to the
- indices for the currently selected group, or all groups defined over
- the alignment.
+ href="../calculations/tree.html">phylogenetic tree
+ partitioning</a>). The option 'apply to all groups' controls whether
+ the contrast slider value will be applied to the indices for the
+ currently selected group, or all groups defined over the alignment.
</p>
</body>
</html>
the background colour.</p>
<p>
The <strong>"Colour→<a
- href="../colourSchemes/textcolour.html"
- >Colour Text...</a>"
+ href="../colourSchemes/textcolour.html">Colour Text...</a>"
</strong> entry opens a dialog box to set a different text colour for light
and dark background, and the intensity threshold for transition
between them.
on its helices. The helices are determined from the secondary
structure line in the Stockholm file (#GC SS_cons) written in WUSS
notation that specifies base pairing. See <a
- href="http://en.wikipedia.org/wiki/Stockholm_format"
- > Wikipedia</a> or <a
- href="http://jalview-rnasupport.blogspot.com/2010/06/parsing-wuss-notation-of-rna-secondary.html"
- > Jalview RNA Support Blog</a> for more information about Stockholm
+ href="http://en.wikipedia.org/wiki/Stockholm_format">
+ Wikipedia</a> or <a
+ href="http://jalview-rnasupport.blogspot.com/2010/06/parsing-wuss-notation-of-rna-secondary.html">
+ Jalview RNA Support Blog</a> for more information about Stockholm
files and WUSS notation.
</p>
Select "Colour"
clicking the left mouse button and pressing a combination of either
shift and control (or the alt, option or apple key on Macs) and
dragging the mouse. Pressing <em>F2</em> toggles the alternative <a
- href="../features/cursorMode.html"
- >'Cursor mode'</a> keyboard editing facility, where the space bar and
- delete keys add and remove gaps at the current editing position. The
- key strokes for both these modes are summarised in the <a
- href="../keys.html"
- >keystrokes table</a>.
+ href="../features/cursorMode.html">'Cursor mode'</a> keyboard
+ editing facility, where the space bar and delete keys add and remove
+ gaps at the current editing position. The key strokes for both these
+ modes are summarised in the <a href="../keys.html">keystrokes
+ table</a>.
</p>
<p>
<strong>Tip:</strong> For large alignments, deselect "Calculate
<strong>Types of annotation</strong>
<ul>
<li><a name="seqannots"><strong>Sequence
- associated annotation.</strong></a><br/>Data displayed on sequence annotation
- rows are associated with the positions of a sequence. Often this
- is 'Reference annotation' such as secondary structure information
- derived from 3D structure data, or from the results of sequence
- based prediction of <a href="../webServices/jnet.html">secondary
+ associated annotation.</strong></a><br />Data displayed on sequence
+ annotation rows are associated with the positions of a sequence.
+ Often this is 'Reference annotation' such as secondary structure
+ information derived from 3D structure data, or from the results of
+ sequence based prediction of <a href="../webServices/jnet.html">secondary
structure</a> and <a href="../webServices/proteinDisorder.html">disorder</a>.
If reference annotation is available for a the currently selected
sequences, it can be shown by selecting the <strong>Add
Reference Annotation</strong> option in the sequence or selection popup
menu.</li>
- <li><strong>Group associated annotation.</strong><br/>Data can be associated with
- groups defined on the alignment. If sequence groups are defined, <a
- href="../calculations/conservation.html">Conservation</a> and <a
- href="../calculations/consensus.html">Consensus</a> annotation can
- be enabled for each group from the <a
+ <li><strong>Group associated annotation.</strong><br />Data can
+ be associated with groups defined on the alignment. If sequence
+ groups are defined, <a href="../calculations/conservation.html">Conservation</a>
+ and <a href="../calculations/consensus.html">Consensus</a>
+ annotation can be enabled for each group from the <a
href="../menus/alwannotation.html">Annotations menu</a>, or can be
- imported from a Jalview <a href="annotationsFormat.html">Annotations
- file</a>.</li>
- <li><strong>Alignment associated annotation.</strong><br />Annotation rows
- associated with columns on the alignment are simply 'alignment
- annotation'. Controls allow you to <a href="#iaannot">interactively create
- alignment annotation</a> to add labels and symbols to alignment columns.
- Jalview's consensus, conservation and quality calculations also
- create histogram and sequence logo annotations on the alignment.
- </li>
+ imported from a Jalview <a href="annotationsFormat.html">Annotations
+ file</a>.</li>
+ <li><strong>Alignment associated annotation.</strong><br />Annotation
+ rows associated with columns on the alignment are simply
+ 'alignment annotation'. Controls allow you to <a href="#iaannot">interactively
+ create alignment annotation</a> to add labels and symbols to
+ alignment columns. Jalview's consensus, conservation and quality
+ calculations also create histogram and sequence logo annotations
+ on the alignment.</li>
</ul>
<p>
<strong>Importing and exporting annotation</strong><br />
groups of annotation rows can be shown or hidden using the pop-up
menu obtained by right-clicking the label. You can also reorder them
by dragging the label to a new position with the left mouse button.
- The
- <strong>Annotations</strong> menu provides settings controlling the
- ordering and display of sequence, group and alignment associated
- annotation. The
- <strong>Colour by annotation</strong> option in the colour menu
- allows annotation to be used to
- <a href="../colourSchemes/annotationColouring.html">shade the
- alignment</a>. Annotations can also be used to
- <a href="../features/columnFilterByAnnotation.html">select or
- hide columns</a> via the dialog opened from the
- <strong>Selection</strong> menu.
+ The <strong>Annotations</strong> menu provides settings controlling
+ the ordering and display of sequence, group and alignment associated
+ annotation. The <strong>Colour by annotation</strong> option in the
+ colour menu allows annotation to be used to <a
+ href="../colourSchemes/annotationColouring.html">shade the
+ alignment</a>. Annotations can also be used to <a
+ href="../features/columnFilterByAnnotation.html">select or
+ hide columns</a> via the dialog opened from the <strong>Selection</strong>
+ menu.
</p>
<p>
<strong>Sequence Highlighting and Selection from Annotation</strong>
using the view's standard font size.</em></li>
</ul>
<p>
- <strong>Editing labels and secondary structure annotation rows</strong>
+ <strong>Editing labels and secondary structure annotation
+ rows</strong>
</p>
<p>
Use the <strong>left mouse button</strong> to select a position
along the row that are to be annotated - these regions will be
- coloured red. Press <strong>Control</strong> or <strong>shift</strong> in
- combination with the left-click to either select an additional position,
- or a range of positions on the alignment.
+ coloured red. Press <strong>Control</strong> or <strong>shift</strong>
+ in combination with the left-click to either select an additional
+ position, or a range of positions on the alignment.
</p>
<p>
Once positions have been selected, use the <strong>right
- mouse button</strong> and select one of the following from the <strong>annotation menu</strong>:
+ mouse button</strong> and select one of the following from the <strong>annotation
+ menu</strong>:
</p>
<ul>
- <li>Helix<br>
- <em>Marks selected positions with a helix glyph (a red oval),
- and optional text label (see below). A dialog box will open for
- you to enter the text. Consecutive ovals will be rendered as an
- unbroken red line.</em>
+ <li>Helix<br> <em>Marks selected positions with a
+ helix glyph (a red oval), and optional text label (see below). A
+ dialog box will open for you to enter the text. Consecutive
+ ovals will be rendered as an unbroken red line.</em>
</li>
- <li>Sheet<br>
- <em>Marks selected positions with a sheet glyph (a green arrow
- oriented from left to right), and optional text label (see
- below). A dialog box will open for you to enter the text.
- Consecutive arrows will be joined together to form a single
- green arrow.</em>
+ <li>Sheet<br> <em>Marks selected positions with a
+ sheet glyph (a green arrow oriented from left to right), and
+ optional text label (see below). A dialog box will open for you
+ to enter the text. Consecutive arrows will be joined together to
+ form a single green arrow.</em>
</li>
<li><a name="rna">RNA Helix</a> (only shown when working with
nucleotide sequences)<br> <em>Marks selected positions
region pairs with bases to the left of the highlighted columns.
Other kinds of base-pair annotation are also supported (e.g. 'A'
and 'a', or '<' and '>'), and Jalview will suggest an
- appropriate symbol based on the closest unmatched
- parenthesis to the left.<br />If any brackets do not match up, then an
- orange square will highlight the first position where a bracket
- was found not to match.
+ appropriate symbol based on the closest unmatched parenthesis to
+ the left.<br />If any brackets do not match up, then an orange
+ square will highlight the first position where a bracket was
+ found not to match.
</em></li>
- <li>Label<br>
- <em>Set the text label at the selected positions. A dialog box
- will open for you to enter the text. If more than one
- consecutive position is marked with the same label, only the
- first position's label will be rendered.</em>
+ <li>Label<br> <em>Set the text label at the selected
+ positions. A dialog box will open for you to enter the text. If
+ more than one consecutive position is marked with the same
+ label, only the first position's label will be rendered.</em>
</li>
- <li>Colour<br>
- <em>Changes the colour of the annotation text label.</em>
+ <li>Colour<br> <em>Changes the colour of the
+ annotation text label.</em>
</li>
- <li>Remove Annotation<br>
- <em>Blanks any annotation at the selected positions on the row.
- Note: <strong>This cannot be undone</strong>
+ <li>Remove Annotation<br> <em>Blanks any annotation
+ at the selected positions on the row. Note: <strong>This
+ cannot be undone</strong>
</em>
</li>
</ul>
<em>Current Limitations</em>
</p>
<p>
- The Jalview user interface does not support interactive
- creation and editing of quantitative annotation (histograms and line
- graphs), or to create annotation associated with a specific
- sequence or group. It is also incapable of annotation grouping or changing
- the style of existing annotation (e.g. to change between line or bar
- charts, or to make multiple line graphs). These annotation
- capabilities are only possible by the import of an <a
- href="annotationsFormat.html">Annotation file</a>.<br>
+ The Jalview user interface does not support interactive creation and
+ editing of quantitative annotation (histograms and line graphs), or
+ to create annotation associated with a specific sequence or group.
+ It is also incapable of annotation grouping or changing the style of
+ existing annotation (e.g. to change between line or bar charts, or
+ to make multiple line graphs). These annotation capabilities are
+ only possible by the import of an <a href="annotationsFormat.html">Annotation
+ file</a>.<br>
</p>
</body>
</html>
Alignment annotations can be imported onto an alignment since
version 2.08 of Jalview, via an annotations file. It is a simple
ASCII text file consisting of tab delimited records similar to the <a
- href="featuresFormat.html"
- >Sequence Features File</a>, and introduced primarily for use with the
- Jalview applet.
+ href="featuresFormat.html">Sequence Features File</a>, and
+ introduced primarily for use with the Jalview applet.
</p>
<p>
alignment window.
</p>
<p>
- <strong>THE ANNOTATION FILE FORMAT</strong> <br />An annotation file
- consists of lines containing an instruction followed by tab
+ <strong>THE ANNOTATION FILE FORMAT</strong> <br />An annotation
+ file consists of lines containing an instruction followed by tab
delimited fields. Any lines starting with "#" are
considered comments, and ignored. The sections below describe the
structure of an annotation file.
</ul>
<p>
At the end of this document, you can also find notes on <a
- href="#compatibility"
- >compatibility</a> of annotation files across different versions of
- Jalview. An <a href="#exampleann">example annotation file</a> is
- also provided along with instructions on how to import it to
- Jalview.
+ href="#compatibility">compatibility</a> of annotation files
+ across different versions of Jalview. An <a href="#exampleann">example
+ annotation file</a> is also provided along with instructions on how to
+ import it to Jalview.
</p>
<hr />
<p>
followed by a <em>description</em> for the row, which is shown in a
tooltip when the user mouses over the annotation row's label. Since
Jalview 2.7, the description field may also contain HTML tags (in
- the same way as a <a href="featuresFormat.html">sequence feature's</a>
- label), providing the text is enclosed in an <html/> tag.
+ the same way as a <a href="featuresFormat.html">sequence
+ feature's</a> label), providing the text is enclosed in an
+ <html/> tag.
<ul>
<em>Please note: URL links embedded in HTML descriptions are
not yet supported.</em>
<em>GRAPH_TYPE</em>. The allowed values of <em>GRAPH_TYPE</em> and
corresponding interpretation of each <em>Value</em> are shown below:
+
<ul>
<li><strong>BAR_GRAPH</strong><br> Plots a histogram with
</ul>
</p>
<p>
- <strong><a name="groupdefs">SEQUENCE_GROUP</a></strong><br /> Groups
- of sequences and column ranges can be defined using a tab delimited
- statement like:
+ <strong><a name="groupdefs">SEQUENCE_GROUP</a></strong><br />
+ Groups of sequences and column ranges can be defined using a tab
+ delimited statement like:
</p>
<pre>SEQUENCE_GROUP	Group_Name	Group_Start	Group_End	<em>Sequences</em>
</pre>
<tr>
<td width="50%">hide</td>
<td>Boolean (default false) indicating whether the rows in
- this group should be marked as hidden.<br />
- <em>Note:</em> if the group is sequence associated (specified by
+ this group should be marked as hidden.<br /> <em>Note:</em>
+ if the group is sequence associated (specified by
SEQUENCE_REF), then all members will be hidden and marked as
represented by the reference sequence.
</td>
</p>
<p>
The BioJSON specification is published at <a
- href="http://jalview.github.io/biojson/"
- >http://jalview.github.io/biojson/</a>.
+ href="http://jalview.github.io/biojson/">http://jalview.github.io/biojson/</a>.
</p>
<p>
<em>Import of BioJSON data from HTML pages</em>
You can set a default choice of Jmol or Chimera structure viewer in
<a href="preferences.html#structure"> Preferences</a>. You can also
optionally specify the path to the Chimera program here (if it
- differs from the standard paths searched by Jalview).<br />
- <strong>Please make sure your version of Chimera is up to
- date. Jalview requires at least Chimera version 1.11.1</strong>
+ differs from the standard paths searched by Jalview).<br /> <strong>Please
+ make sure your version of Chimera is up to date. Jalview requires
+ at least Chimera version 1.11.1</strong>
</p>
<p>
If you save your Jalview session as a project file, the state of any
Help menu.
<p>
Basic screen operations (see <a
- href="http://www.cgl.ucsf.edu/chimera/current/docs/UsersGuide/mouse.html"
- >Chimera help</a> at
+ href="http://www.cgl.ucsf.edu/chimera/current/docs/UsersGuide/mouse.html">Chimera
+ help</a> at
http://www.cgl.ucsf.edu/chimera/current/docs/UsersGuide/mouse.html
for full details).
<table border="1">
colourschemes.<br>
</strong><em>The remaining entries apply the colourschemes available
from the standard and user defined <a
- href="../colourSchemes/index.html"
- >amino acid colours</a>.
+ href="../colourSchemes/index.html">amino acid
+ colours</a>.
</em></li>
</ul></li>
<li><strong>Chimera<br>
</p>
Jalview and Chimera communicate using Chimera's
<a
- href="http://www.cgl.ucsf.edu/chimera/current/docs/ContributedSoftware/restserver/restserver.html"
- >REST service</a>
+ href="http://www.cgl.ucsf.edu/chimera/current/docs/ContributedSoftware/restserver/restserver.html">REST
+ service</a>
(http://www.cgl.ucsf.edu/chimera/current/docs/ContributedSoftware/restserver/restserver.html).
<br> Technically this requires both Chimera and Jalview to open
ports on the local network, and this may be blocked by Windows
<div align="center">-annotations FILE/URL</div>
</td>
<td>Add precalculated annotations to the alignment. See <a
- href="annotationsFormat.html" target="NEW"
- >Annotation File</a> description.
+ href="annotationsFormat.html" target="NEW">Annotation
+ File</a> description.
</td>
</tr>
<tr>
<td>
<div align="center">-nonews</div>
<td>
- <div align="left">Disable check for <a href="../webServices/newsreader.html">Jalview news</a> on startup (not recommended other than for classroom / demo usage)</div>
+ <div align="left">
+ Disable check for <a href="../webServices/newsreader.html">Jalview
+ news</a> on startup (not recommended other than for classroom /
+ demo usage)
+ </div>
</td>
</tr>
<tr>
The 'Select/Hide by Annotation' window allows columns to be selected
or hidden according to annotation rows on the alignment. The dialog
box is opened <em>via</em> "<strong>Select→Select/Hide
- Columns by Annotation...</strong>", and different filters are
- then presented for filtering data according to the selected annotation
+ Columns by Annotation...</strong>", and different filters are then
+ presented for filtering data according to the selected annotation
row.
</p>
<table>
<pre>java -Djava.ext.dirs=$INSTALL_DIR$/lib -cp $INSTALL_DIR$/jalview.jar jalview.bin.Jalview -open [FILE] </pre>
<p>
Use '-help' to get more information on the <a
- href="clarguments.html"
- >command line arguments</a> that Jalview accepts.
+ href="clarguments.html">command line arguments</a> that
+ Jalview accepts.
</p>
<p> </p>
<p> </p>
<strong>Creating Sequence Features</strong>
<p>
Jalview can create sequence features from the matches of a <a
- href="search.html"
- >regular expression search</a>, or from the currently selected area
- via the <strong>"selection→Create sequence
- feature"</strong> entry in the <a href="../menus/popupMenu.html">selection
- area popup menu</a>. In both cases, the <strong>Create
- Features</strong> dialog box will then be opened:
+ href="search.html">regular expression search</a>, or from the
+ currently selected area via the <strong>"selection→Create
+ sequence feature"</strong> entry in the <a
+ href="../menus/popupMenu.html">selection area popup menu</a>. In
+ both cases, the <strong>Create Features</strong> dialog box will
+ then be opened:
</p>
<p>
<img src="crnewfeature.gif">
<p>
<strong>DAS Sequence Feature Retrieval</strong>
</p>
- <p>
- Jalview includes a client for retrieving sequences and their
- features via the Distributed Annotation System.
- </p>
+ <p>Jalview includes a client for retrieving sequences and their
+ features via the Distributed Annotation System.</p>
<ol>
<li>Open the Feature Settings panel by selecting "View
-> Feature Settings..."</li>
Accession ids for the given sequence names. It is important to
realise that many DAS sources only use UniProt accession ids, rather
than Swissprot/UniProt sequence names.<br> The <a
- href="../webServices/dbreffetcher.html"
- >database reference fetcher</a> documentation describes how Jalview
- discovers what database references are appropriate for the sequences
- in the alignment.
+ href="../webServices/dbreffetcher.html">database
+ reference fetcher</a> documentation describes how Jalview discovers
+ what database references are appropriate for the sequences in the
+ alignment.
<ul>
<li><em>Note</em><br> Please remember to save your
alignment if either the start/end numbering, or the sequence IDs
<p>
<em>DAS support was introduced in Jalview Version 2.1.</em>
</p>
- <br/>
+ <br />
<p>
- <em>The DAS registry at http://www.dasregistry.org was decommissioned early in 2015. An unmaintained mirror is currently hosted at http://www.ebi.ac.uk/das-srv/registry/.</em>
+ <em>The DAS registry at http://www.dasregistry.org was
+ decommissioned early in 2015. An unmaintained mirror is currently
+ hosted at http://www.ebi.ac.uk/das-srv/registry/.</em>
</p>
<p>
</body>
</p>
<p>
Jalview can retrieve sequences and features from many <a
- href="http://biodas.org/"
- >DAS</a> sources. The DAS sources that it uses are discovered and
- selected <em>via</em> the DAS settings panel, opened either from the
- <a href="featuresettings.html">View→Feature Settings</a>
- dialog box from the alignment window's menu bar, or the <a
- href="featuresettings.html"
- >Tools→Preferences</a> dialog box opened from the Desktop menu
- bar.
+ href="http://biodas.org/">DAS</a> sources. The DAS sources
+ that it uses are discovered and selected <em>via</em> the DAS
+ settings panel, opened either from the <a
+ href="featuresettings.html">View→Feature Settings</a> dialog
+ box from the alignment window's menu bar, or the <a
+ href="featuresettings.html">Tools→Preferences</a>
+ dialog box opened from the Desktop menu bar.
</p>
<p>
<img src="das.gif">
<!-- and <strong>Feature Group</strong> -->
pull down menu. In addition to the Name, group, colour and
description attributes described for the <a
- href="creatinFeatures.html"
- >new feature dialog box</a>, a feature's start and end position can be
- changed either by entering a new position directly or by using the
- adjacent up and down buttons.
+ href="creatinFeatures.html">new feature dialog box</a>, a
+ feature's start and end position can be changed either by entering a
+ new position directly or by using the adjacent up and down buttons.
</p>
<p>
Select <strong>Amend</strong> to update the feature, <strong>Delete</strong>
Two types of ENSEMBL source are provided. ENSEMBL queries the main
ENSEMBL warehouse containing data for higher eukaryotes, and
EnsemblGenomes, which queries Ensembl Pathogens, and other
- warehouses.<br />
- <em>Ensembl support is new in Jalview, and we expect to merge
- these sources in a future release.</em>
+ warehouses.<br /> <em>Ensembl support is new in Jalview, and
+ we expect to merge these sources in a future release.</em>
</p>
<p>
- <strong>General Use</strong><br /> If you have a set of Ensembl gene
- or transcript IDs, then you can retrieve them <em>via</em> the
+ <strong>General Use</strong><br /> If you have a set of Ensembl
+ gene or transcript IDs, then you can retrieve them <em>via</em> the
sequence fetcher dialog opened after selecting the most appropriate
source (either 'ENSEMBL', or Ensembl Genomes). However, Jalview's
Ensembl client has a couple of additional capabilities:
<p>
If your sequence annotation is already available in GFF Format (see
- <a href="http://gmod.org/wiki/GFF2">gmod.org/wiki/GFF2</a>),
- then you can leave it as is, after first adding a line containing
- only 'GFF' after any Jalview feature colour definitions (<em>this
+ <a href="http://gmod.org/wiki/GFF2">gmod.org/wiki/GFF2</a>), then
+ you can leave it as is, after first adding a line containing only
+ 'GFF' after any Jalview feature colour definitions (<em>this
mixed format capability was added in Jalview 2.6</em>). Alternately,
you can use Jalview's own sequence feature annotation format, which
additionally allows HTML and URLs to be directly attached to each
</pre>
This format allows two alternate ways of referring to a sequence,
- either by its text ID, or its index (base 0) in an associated alignment.
- Normally, sequence features are associated with sequences rather than
- alignments, and the sequenceIndex field is given as "-1". In
- order to specify a sequence by its index in a particular alignment,
- the sequenceId should be given as "ID_NOT_SPECIFIED",
- otherwise the sequenceId field will be used in preference to the
- sequenceIndex field.
+ either by its text ID, or its index (base 0) in an associated
+ alignment. Normally, sequence features are associated with sequences
+ rather than alignments, and the sequenceIndex field is given as
+ "-1". In order to specify a sequence by its index in a
+ particular alignment, the sequenceId should be given as
+ "ID_NOT_SPECIFIED", otherwise the sequenceId field will be
+ used in preference to the sequenceIndex field.
</p>
description line will be translated into URL links. A link symbol
will be displayed adjacent to any feature which includes links, and
these are made available from the <a
- href="../menus/popupMenu.html#sqid.popup"
- >links submenu</a> of the popup menu which is obtained by
- right-clicking when a link symbol is displayed in the tooltip.<br>
- <em>Non-positional features</em><br> Specify the <em>start</em>
- and <em>end</em> for a feature to be <strong>0</strong> in order to
- attach it to the whole sequence. Non-positional features are shown
- in a tooltip when the mouse hovers over the sequence ID panel, and
- any embedded links can be accessed from the popup menu.<br /> <em>Scores</em><br>
+ href="../menus/popupMenu.html#sqid.popup">links submenu</a>
+ of the popup menu which is obtained by right-clicking when a link
+ symbol is displayed in the tooltip.<br> <em>Non-positional
+ features</em><br> Specify the <em>start</em> and <em>end</em> for
+ a feature to be <strong>0</strong> in order to attach it to the
+ whole sequence. Non-positional features are shown in a tooltip when
+ the mouse hovers over the sequence ID panel, and any embedded links
+ can be accessed from the popup menu.<br /> <em>Scores</em><br>
Scores can be associated with sequence features, and used to sort
sequences or shade the alignment (this was added in Jalview 2.5).
The score field is optional, and malformed scores will be ignored.
</p>
<p>
<strong><em>Feature settings pop-up menu</em></strong><br> <strong>Right-click</strong>
- on a feature to open a pop-up menu that allows you to<ul><li>Hide, show and
- select columns containing that feature</li><li>Sort the alignment or
- current selection using that feature type (see below)</li><li>Toggle the
- type of colouring used for the feature</li></ul><p>Features may be highlighted
- with either a single colour or a <a href="featureschemes.html">feature
- colourscheme</a> based on either the scores associated with that
- feature or from the feature's description (e.g. to distinguish
- different names associated with a DOMAIN feature).
+ on a feature to open a pop-up menu that allows you to
+ <ul>
+ <li>Hide, show and select columns containing that feature</li>
+ <li>Sort the alignment or current selection using that feature
+ type (see below)</li>
+ <li>Toggle the type of colouring used for the feature</li>
+ </ul>
+ <p>
+ Features may be highlighted with either a single colour or a <a
+ href="featureschemes.html">feature colourscheme</a> based on
+ either the scores associated with that feature or from the feature's
+ description (e.g. to distinguish different names associated with a
+ DOMAIN feature).
</p>
<p>
- <strong><a name="sortbyfeature">Ordering alignment by features</a></strong><br> The 'Seq
- Sort by Score' and 'Seq Sort by Density' buttons will sort the
- alignment based on the average score or total number of currently
- active features and groups on each sequence. To order the alignment
- using a specific feature type, use the <em>sort by ..</em> entries
- in the pop-up menu for that type.<br> <em>Feature sorting
- and graduated feature colouring were introduced in Jalview 2.5</em>
+ <strong><a name="sortbyfeature">Ordering alignment by
+ features</a></strong><br> The 'Seq Sort by Score' and 'Seq Sort by
+ Density' buttons will sort the alignment based on the average score
+ or total number of currently active features and groups on each
+ sequence. To order the alignment using a specific feature type, use
+ the <em>sort by ..</em> entries in the pop-up menu for that type.<br>
+ <em>Feature sorting and graduated feature colouring were
+ introduced in Jalview 2.5</em>
</p>
<p>
ordering based on the average length of each feature type.
</p>
<p>
- The <strong><em>transparency slider setting</em></strong> controls the visibility
- of features rendered below other features. Reducing the transparency
- will mean that features at the top of the list can obscure features
- lower down, and increasing it allows the user to 'see through' the
- upper layers of a set of features.
+ The <strong><em>transparency slider setting</em></strong> controls
+ the visibility of features rendered below other features. Reducing
+ the transparency will mean that features at the top of the list can
+ obscure features lower down, and increasing it allows the user to
+ 'see through' the upper layers of a set of features.
</p>
<p>
<strong><em>You can save all features, with their
public methods of the jalview class hierarchy can be called from
Groovy scripts. In addition, the following objects are also defined:
+
<ul>
<li><strong>Jalview</strong> - this is bound to <code>jalview.bin.Jalview</code>.<br />Useful
def seq = alignment.getSequenceAt(0);
</pre>
</li>
- <li>If you wanted to do the same thing from the command line, you can refer to
- alignment that was just loaded with currentAlFrame:<br>
- <pre>
+ <li>If you wanted to do the same thing from the command line,
+ you can refer to alignment that was just loaded with
+ currentAlFrame:<br> <pre>
print currentAlFrame.getTitle();</pre>
</ul>
<p>
<li><strong><a href="../calculations/tree.html">Tree</a></strong>,
<strong><a href="../calculations/pairwise.html">pairwise
alignment</a></strong> and <strong><a
- href="../calculations/pca.html"
- >PCA</a></strong> calculations will only be performed using the <em>visible</em>
- parts of the alignment.</li>
+ href="../calculations/pca.html">PCA</a></strong> calculations will
+ only be performed using the <em>visible</em> parts of the
+ alignment.</li>
<li><a href="../webServices/msaclient.html">Multiple
Sequence Alignments</a> are performed locally on on each visible
chunk of the input, and concatenated with the hidden regions to
structures opened by entries in the <strong>"Structure"</strong>
submenu in the <a href="../menus/popupMenu.html">sequence id
pop-up menu</a> (if you can't see this, then you need to <a
- href="viewingpdbs.html"
- >associate a PDB structure</a> with the sequence). Jmol is available
- from the Jalview desktop and should also run in the JalviewLite
- applet, providing the browser supports Java 1.5. If Jmol is not
- available, then the original <a href="pdbviewer.html">internal
- pdb viewer</a> will be used as a fallback.
+ href="viewingpdbs.html">associate a PDB structure</a> with
+ the sequence). Jmol is available from the Jalview desktop and should
+ also run in the JalviewLite applet, providing the browser supports
+ Java 1.5. If Jmol is not available, then the original <a
+ href="pdbviewer.html">internal pdb viewer</a> will be used as a
+ fallback.
</p>
<!-- <p>The following menu entries are provided for viewing structure data<br>
<ul>
colourschemes.<br>
</strong><em>The remaining entries apply the colourschemes available
from the standard and user defined <a
- href="../colourSchemes/index.html"
- >amino acid colours</a>.
+ href="../colourSchemes/index.html">amino acid
+ colours</a>.
</em></li>
</ul></li>
<li><strong>Jmol<br>
Jmol scripting console.</p>
<p>
The state of each Jmol display is stored within <a
- href="jalarchive.html"
- >Jalview archives</a> as a Jmol state recovery script file. This means
- that any Jmol visualization effects that you add beyond those
- provided by Jalview will be able to be stored and recovered along
- with the displayed alignments in Jalview.
+ href="jalarchive.html">Jalview archives</a> as a Jmol state
+ recovery script file. This means that any Jmol visualization effects
+ that you add beyond those provided by Jalview will be able to be
+ stored and recovered along with the displayed alignments in Jalview.
</p>
<p>
<strong>More Information</strong>
Jmol is a sophisticated program in its own right, with its own
command console and scripting language. Only the essentials have
been described here - the interested reader is referred to <a
- href="http://jmol.sourceforge.net/docs/"
- >Jmol's own comprehensive online documentation</a>.
+ href="http://jmol.sourceforge.net/docs/">Jmol's own
+ comprehensive online documentation</a>.
</p>
</p>
</body>
File'. This format was developed by the PDB consortium and the
International Union of Crystallography (IUCr), based on
Crystallographic Information File (CIF), a format used for describing
- the structures of small molecules.<br/>mmCIF became the recommended format
- for the exchange of biomacromolecular structures in 2014.
+ the structures of small molecules.
+ <br />mmCIF became the recommended format for the exchange of
+ biomacromolecular structures in 2014.
<p>
<strong>mmCIF and Jalview</strong> <br />Since Jalview 2.10, mmCIF
is used for structures downloaded from the PDB. This means:
<p>
A tree calculated on a particular view, or loaded onto it, is by
default associated with just that view. However, the <a
- href="../calculations/treeviewer.html"
- >Tree Viewer's</a> <strong>"View→Associate
+ href="../calculations/treeviewer.html">Tree Viewer's</a> <strong>"View→Associate
leaves"</strong> submenu allows a tree's view association to be
changed to to any or all other views.
</p>
<strong>"File →Fetch Sequences"</strong>).
</p>
<img src="pdbseqfetcher.png" align="left"
- alt="PDB sequence fetcher (introduced in Jalview 2.9)"
- />
+ alt="PDB sequence fetcher (introduced in Jalview 2.9)" />
<p>
<strong>Searching the PDB Database</strong>
</p>
<p>
<ul>
- <li><strong>Searching a specific PDB field</strong><br />If you
- want to find structures based on a specific PDB metadata field,
- you can select it from the drop-down menu.</li>
+ <li><strong>Searching a specific PDB field</strong><br />If
+ you want to find structures based on a specific PDB metadata
+ field, you can select it from the drop-down menu.</li>
<li><strong>Retrieving a unique chain for a PDB entry</strong><br>To
retrieve a specific chain for a PDB entry, append the PDB ID with
a colon followed by the chain code in the search box.<br /> e.g
pop-up menu</a>. The internal PDB viewer is not able to show
superpositions, so no other options are provided. Structures can
only be viewed for sequences which have an <a
- href="viewingpdbs.html"
- >associated PDB structure</a>, and the PDB Viewer will only be
- associated with the particular alignment view from which it was
- opened.
+ href="viewingpdbs.html">associated PDB structure</a>, and the
+ PDB Viewer will only be associated with the particular alignment
+ view from which it was opened.
</p>
<p>
<strong>Controls</strong>
Jalview colourschemes.<br>
</em></strong> The remaining entries apply the colourschemes available from
the standard and user defined <a
- href="../colourSchemes/index.html"
- >amino acid colours</a>.</em></li>
+ href="../colourSchemes/index.html">amino acid
+ colours</a>.</em></li>
</ul></li>
<li><strong>View<br>
</strong><em> These options can be turned off to improve performance
</li>
<li>The <a href="../webServices/webServicesPrefs.html"><strong>"Web
Service"</strong> Preferences</a> tab allows you to configure the <a
- href="http://www.compbio.dundee.ac.uk/jabaws"
- >JABAWS</a> servers that Jalview uses, and change the layout of the
+ href="http://www.compbio.dundee.ac.uk/jabaws">JABAWS</a>
+ servers that Jalview uses, and change the layout of the
alignment's Web Services menu.
</li>
</ul>
</p>
<p>
<em>Open Overview Window</em> - When this is selected, the <a
- href="overview.html"
- >alignment overview</a> panel is opened by default for a new alignment
- window.
+ href="overview.html">alignment overview</a> panel is opened
+ by default for a new alignment window.
</p>
<p>
<em>Show Annotations</em> - If this is selected the new window will
<em>Open file</em> - If this is selected then the default alignment
file will be opened when Jalview is started. You can change the
default file by clicking on file name and either typing in the file
- path or selecting it from the file chooser window.<br />
- <em>Note: The default example alignment is updated periodically
- to demonstrate new features in Jalview.</em>
+ path or selecting it from the file chooser window.<br /> <em>Note:
+ The default example alignment is updated periodically to
+ demonstrate new features in Jalview.</em>
</p>
<p>
<a name="colours"><strong>"Colours"
<p>
<em>Annotation Shading Default</em> - set the default minimum and
maximum colours used when <a
- href="../colourSchemes/annotationColouring.html"
- >Colour by Annotation...</a> is selected from the alignment window's
- colours menu.
+ href="../colourSchemes/annotationColouring.html">Colour
+ by Annotation...</a> is selected from the alignment window's colours
+ menu.
</p>
<p>
<a name="structure"><strong>"Structure"
<em>URL Link From Sequence ID</em><br> These definitions are
used to generate URLs from a sequence's ID or database cross
references. Read more about <a
- href="../webServices/urllinks.html#urllinks"
- >configuring URL links here</a>.
+ href="../webServices/urllinks.html#urllinks">configuring
+ URL links here</a>.
</p>
<p>
<em>Default Browser (Unix)</em><br> Its difficult in Java to
</p>
<p>
When this option is enabled, Jalview embeds <a
- href="bioJsonFormat.html"
- >BioJSON</a> data within HTML files exported from Jalview at
- generation time. This enables the exported HTML files to be
- extracted and imported back into the Jalview desktop application at
- a later time.
+ href="bioJsonFormat.html">BioJSON</a> data within HTML files
+ exported from Jalview at generation time. This enables the exported
+ HTML files to be extracted and imported back into the Jalview
+ desktop application at a later time.
<p>
<em>Use Modeller Output</em>
</p>
<p>
By default, Jalview will assign a color to each feature based on its
type. These colours can be changed from the <a
- href="featuresettings.html"
- >feature settings</a> and <a href="editingFeatures.html">amend
- features</a> dialog boxes. Since Jalview 2.5, it is also possible to
- define <a href="featureschemes.html">feature colourschemes</a> to
- shade features based on their associated scores or text labels.
+ href="featuresettings.html">feature settings</a> and <a
+ href="editingFeatures.html">amend features</a> dialog boxes. Since
+ Jalview 2.5, it is also possible to define <a
+ href="featureschemes.html">feature colourschemes</a> to shade
+ features based on their associated scores or text labels.
</p>
<p>
<strong>Sequence Feature Groups</strong>
organised into groups, which may indicate that the features were all
retrieved from the same database (such as UniProt features), or
generated by the same analysis process (as might be specified in a <a
- href="featuresFormat.html"
- >sequence features file</a>).
+ href="featuresFormat.html">sequence features file</a>).
</p>
<p>
<strong>Sequence Feature Inheritance</strong>
<p>
Once sequence features have been loaded, their display can be fully
controlled using the alignment window's <a
- href="featuresettings.html"
- >Sequence Feature Settings</a> dialog box. Feature colour schemes and
- display parameters are unique to a particular alignment, so it is
- possible to colour the same sequence features differently in
- different alignment views.<br> Since Jalview 2.1, it is
- possible to add <a href="dassettings.html">DAS features</a> to an
- alignment via the DAS tabbed pane of the feature settings window.
+ href="featuresettings.html">Sequence Feature Settings</a>
+ dialog box. Feature colour schemes and display parameters are unique
+ to a particular alignment, so it is possible to colour the same
+ sequence features differently in different alignment views.<br>
+ Since Jalview 2.1, it is possible to add <a href="dassettings.html">DAS
+ features</a> to an alignment via the DAS tabbed pane of the feature
+ settings window.
</p>
<p>
<strong>View→Sequence ID Tooltip→Show
Precalculated Sequence Features may be added to an alignment from
the command line, drag and drop, or from the "File→Load
Features / Annotations" menu item. See the <a
- href="featuresFormat.html"
- >Features File Format</a> for more details.
+ href="featuresFormat.html">Features File Format</a> for more
+ details.
</p>
</body>
</html>
Institute, or, since Jalview 2.4, DAS servers capable of the <em>sequence</em>
command (configured in <a href="dassettings.html">DAS settings</a>).
</p>
- <p>The Sequence Fetcher can be opened via the
- "File" menu on the main desktop in order to retrieve
- sequences as a new alignment, or opened via the "File"
- menu of an existing alignment to import additional sequences. There
- may be a short delay when the sequence fetcher is first opened,
- whilst Jalview compiles the list of available sequence datasources
- from the currently defined DAS server registry.</p>
+ <p>The Sequence Fetcher can be opened via the "File"
+ menu on the main desktop in order to retrieve sequences as a new
+ alignment, or opened via the "File" menu of an existing
+ alignment to import additional sequences. There may be a short delay
+ when the sequence fetcher is first opened, whilst Jalview compiles
+ the list of available sequence datasources from the currently
+ defined DAS server registry.</p>
<p>
- Every time a new fetcher is opened, you will need to <strong>select the database you want to retrieve
- sequences</strong> from the database chooser.
+ Every time a new fetcher is opened, you will need to <strong>select
+ the database you want to retrieve sequences</strong> from the database
+ chooser.
</p>
<img src="selectfetchdb.gif" align="left" width="480" height="204"
- alt="Database selection dialog for fetching sequences (introduced in Jalview 2.8)"
- >
- <p>The databases are shown as a tree, and ordered alphabetically;
+ alt="Database selection dialog for fetching sequences (introduced in Jalview 2.8)">
+ <p>
+ The databases are shown as a tree, and ordered alphabetically;
tooltips are shown if you mouse over some sources, explaining what
the database will retrieve. You can select one by using the up/down
arrow keys and hitting return, or by double clicking with the mouse.
- <br/><em>If you have DAS sources enabled, then you may have several sources
- for the same type of sequence identifier, and these will be grouped
- together in a sub-branch branch labeled with the identifier.</em></p>
+ <br />
+ <em>If you have DAS sources enabled, then you may have several
+ sources for the same type of sequence identifier, and these will
+ be grouped together in a sub-branch branch labeled with the
+ identifier.</em>
+ </p>
<p>Once you have selected a sequence database, its fetcher dialog
will open. Jalview provides two types of dialog:</p>
- <ol><li><strong>The Free-text Search Interface</strong>
-
- <br/>Free-text search clients are provided for PDB (Since 2.9), and
- UniProt (Since 2.10). They provide access to each database's own
- query system, enabling you to retrieve data by accession, free text
- description, or any other type of supported field. For full details,
- see each client's help page:
- <ul>
- <li><a href="pdbsequencefetcher.html">PDB Sequence Fetcher</a></li>
- <li><a href="uniprotsequencefetcher.html">UniProt Sequence
- Fetcher</a></li>
- </ul>
- </li>
- <li><strong>Accession based sequence retrieval</strong>
- <br/>
+ <ol>
+ <li><strong>The Free-text Search Interface</strong> <br />Free-text
+ search clients are provided for PDB (Since 2.9), and UniProt
+ (Since 2.10). They provide access to each database's own query
+ system, enabling you to retrieve data by accession, free text
+ description, or any other type of supported field. For full
+ details, see each client's help page:
+ <ul>
+ <li><a href="pdbsequencefetcher.html">PDB Sequence
+ Fetcher</a></li>
+ <li><a href="uniprotsequencefetcher.html">UniProt
+ Sequence Fetcher</a></li>
+ </ul></li>
+ <li><strong>Accession based sequence retrieval</strong> <br />
- <img src="seqfetcher.gif" align="center"
- alt="The Jalview Sequence Fetcher Dialog Box"><br/>
- To retrieve sequences, simply <strong>enter one or more accession ids</strong> (as a semi-colon
- separated list), or press the "Example" button to paste the
- example accession for the currently selected database into the
- retrieval box. Finally, press "OK" to initiate the
- retrieval.
- </li>
+ <img src="seqfetcher.gif" align="center"
+ alt="The Jalview Sequence Fetcher Dialog Box"><br /> To
+ retrieve sequences, simply <strong>enter one or more
+ accession ids</strong> (as a semi-colon separated list), or press the
+ "Example" button to paste the example accession for the
+ currently selected database into the retrieval box. Finally, press
+ "OK" to initiate the retrieval.</li>
</ol>
<p>
<strong>Only retrieving part of a sequence</strong>
<body>
<p>
<strong>Mapping Between Different Sequences</strong>
- </p><!-- TODO: review and check if this page is really needed -->
+ </p>
+ <!-- TODO: review and check if this page is really needed -->
<p>A new feature in Jalview 2.3 is the ability to map between
sequences in different domains, based on alignment, or by the use of
explicit mappings provided by databases.</p>
will generate a mapping using the built-in Needleman and Wunsch
global alignment algorithm. This is how sequence-structure mappings
were created before version 2.10.</p>
- <p><strong>Controlling and troubleshooting SIFTS mappings</strong> <br />
+ <p>
+ <strong>Controlling and troubleshooting SIFTS mappings</strong> <br />
Configuration options controlling whether SIFTS mappings are used
can be found in the <strong>Tools → Preferences →
Structure tab</strong>, under 'Sequence ↔ Structure method'.<br /> <em>Note:</em>
<strong>Multi-Chain Mappings</strong> <br />SIFTS gives Jalview the
ability to display multi-chain mappings between UniProt sequences
and PDB structure data. This is important when working with
- multimeric proteins, when the biological assembly can contain several
- structures for the same protein sequence. Multi-chain mapping allows
- all residues in a structure to be located in the alignment, and
- also, when shading the structure by sequence colours, enables
- conservation patterns between oligomer interfaces to be explored.
+ multimeric proteins, when the biological assembly can contain
+ several structures for the same protein sequence. Multi-chain
+ mapping allows all residues in a structure to be located in the
+ alignment, and also, when shading the structure by sequence colours,
+ enables conservation patterns between oligomer interfaces to be
+ explored.
</p>
<p>To see this in action, Retrieve the UniProt sequence
FER1_MAIZE, and then view one of its structures: 3B2F. Mousing over
<Strong>Viewing Mapping Output</Strong> <br /> The mapping provided
by the SIFTS record is accessible via <strong>File →
View mapping</strong> menu of the structure viewers. The screenshot below
- shows the mapping created between UniProt sequence FER1_MAIZE and proteins in PDB 3B2F, which reports thattwo chains
- were mapped. The mapping method is also reported (highlighted with red border).
+ shows the mapping created between UniProt sequence FER1_MAIZE and
+ proteins in PDB 3B2F, which reports thattwo chains were mapped. The
+ mapping method is also reported (highlighted with red border).
<p>
 <img src="sifts_mapping_output.png" align="left"
- alt="SIFTS mapping output" />
- <br/>
+ alt="SIFTS mapping output" /> <br />
<p>
<em>SIFTS Mapping integration was added in Jalview 2.10</em>
</p>
<ul>
<li>Mouseover or scrolling of either alignment is followed by
the other (unless you turn off <strong><a
- href="../menus/alwview.html"
- >"View→Automatic Scrolling"</a></strong>)
+ href="../menus/alwview.html">"View→Automatic
+ Scrolling"</a></strong>)
</li>
<li>On selecting rows, columns or regions in one alignment, the
corresponding selection is made in the other</li>
<li>Sequence ordering in one alignment (using the cursor, or <strong><a
- href="../calculations/sorting.html"
- >"Calculate→Sort")</a></strong> is also applied to the other
+ href="../calculations/sorting.html">"Calculate→Sort")</a></strong>
+ is also applied to the other
</li>
<li>Editing (gap insertion / deletion) in the protein alignment
is reflected in the cDNA (but not vice versa)</li>
<li>Any trees imported or created with <strong><a
- href="../calculations/tree.html"
- >"Calculate Tree"</a></strong> on one of the views allow both cDNA and
- Protein views to be grouped, coloured or sorted.
+ href="../calculations/tree.html">"Calculate Tree"</a></strong> on
+ one of the views allow both cDNA and Protein views to be grouped,
+ coloured or sorted.
</li>
<li>To allow for the different widths in cDNA and Protein
alignments, the <strong><a href="../menus/alwformat.html">"Format→Font"</a></strong>
</ul>
<p>
An alignment annotation row on the protein alignment shows the <strong><a
- href="../calculations/consensus.html"
- >cDNA consensus</a></strong> for each peptide column.<br /> This consensus may
- reveal variation in nucleotides coding for conserved protein
- residues.
+ href="../calculations/consensus.html">cDNA consensus</a></strong> for
+ each peptide column.<br /> This consensus may reveal variation in
+ nucleotides coding for conserved protein residues.
</p>
<a name="opensplit" />
sample alignment. Note however that if no structures were
auto-discovered, a different interface for manual association will
be invoked as seen in the screenshot below.
-
<p>
<img src="schooser_enter-id.png"
style="width: 464px; height: 369px;">
-
<p>
<strong>Manual selection/association of PDB files with
Sequences</strong>
</p>
<p>To manually associate PDB files with a sequence, select 'From
File', or 'Enter PDB Id' from the drop-down menu:
-
<ul>
<li><strong>From File</strong> - allows you to load a PDB file
from the local machine or network and associate it with the
syntax</a>).
</p>
- <table border="1" width="95%">
- <tr>
- <th>Field</th>
- <th>Example</th>
- <th>Description</th>
- </tr>
- <tr>
- <td>accession</td>
- <td>
- <code>accession:P62988</code>
- </td>
- <td>
- Lists all entries with the primary or secondary
- accession number P62988.
- </td>
- </tr>
- <tr>
- <td>active</td>
- <td>
- <code>active:no </code>
- </td>
- <td>
- Lists all obsolete entries.
- </td>
- </tr>
- <tr>
- <td>annotation</td>
- <td>
- <code>
- annotation:(type:non-positional)
- <br />
- annotation:(type:positional)
- <br />
- annotation:(type:mod_res "Pyrrolidone carboxylic acid" evidence:experimental)
- </code>
- </td>
- <td>
- Lists all entries with:
- <ul>
- <li>any general annotation (comments [CC])</li>
- <li>any sequence annotation (features [FT])</li>
- <li>at least one amino acid modified with a Pyrrolidone carboxylic acid group</li>
- </ul>
- </td>
- </tr>
- <tr>
- <td>author</td>
- <td>
- <code>
- author:ashburner
- </code>
- </td>
- <td>
- Lists all entries with at least one reference co-authored by Michael Ashburner.
- </td>
- </tr>
- <tr>
- <td>cdantigen</td>
- <td>
- <code>
- cdantigen:CD233
- </code>
- </td>
- <td>
- Lists all entries whose cluster of differentiation number is CD233.
- </td>
- </tr>
- <tr>
- <td>citation</td>
- <td>
- <code>
- citation:("intracellular structural proteins")
- <br />
- citation:(author:ashburner journal:nature)
- citation:9169874
- </code>
- </td>
- <td>
- Lists all entries with a literature citation:
- <ul>
- <li>containing the phrase "intracellular structural proteins" in either title or abstract</li>
- <li>co-authored by Michael Ashburner and published in Nature</li>
- <li>with the PubMed identifier 9169874</li>
- </ul>
- </td>
- </tr>
- <tr>
- <td>cluster</td>
- <td>
- <code>
- cluster:UniRef90_A5YMT3
- </code>
- </td>
- <td>
- Lists all entries in the UniRef 90% identity cluster whose
- representative sequence is UniProtKB entry A5YMT3.
- </td>
- </tr>
- <tr>
- <td>count</td>
- <td>
- <code>
- annotation:(type:transmem count:5)<br />
- annotation:(type:transmem count:[5 TO *])<br />
- annotation:(type:cofactor count:[3 TO *])
- </code>
- </td>
- <td>Lists all entries with:
- <ul>
- <li>exactly 5 transmembrane regions</li>
- <li>5 or more transmembrane regions</li>
- <li>3 or more Cofactor comments</li>
- </ul>
- </td>
- </tr>
- <tr>
- <td>created</td>
- <td>
- <code>
- created:[20121001 TO *]<br />
- reviewed:yes AND created:[current TO *]
- </code>
- </td>
- <td>
- Lists all entries created since October 1st 2012.<br />
+ <table border="1" width="95%">
+ <tr>
+ <th>Field</th>
+ <th>Example</th>
+ <th>Description</th>
+ </tr>
+ <tr>
+ <td>accession</td>
+ <td><code>accession:P62988</code></td>
+ <td>Lists all entries with the primary or secondary accession
+ number P62988.</td>
+ </tr>
+ <tr>
+ <td>active</td>
+ <td><code>active:no </code></td>
+ <td>Lists all obsolete entries.</td>
+ </tr>
+ <tr>
+ <td>annotation</td>
+ <td><code>
+ annotation:(type:non-positional) <br />
+ annotation:(type:positional) <br /> annotation:(type:mod_res
+ "Pyrrolidone carboxylic acid" evidence:experimental)
+ </code></td>
+ <td>Lists all entries with:
+ <ul>
+ <li>any general annotation (comments [CC])</li>
+ <li>any sequence annotation (features [FT])</li>
+ <li>at least one amino acid modified with a Pyrrolidone
+ carboxylic acid group</li>
+ </ul>
+ </td>
+ </tr>
+ <tr>
+ <td>author</td>
+ <td><code> author:ashburner </code></td>
+ <td>Lists all entries with at least one reference co-authored
+ by Michael Ashburner.</td>
+ </tr>
+ <tr>
+ <td>cdantigen</td>
+ <td><code> cdantigen:CD233 </code></td>
+ <td>Lists all entries whose cluster of differentiation number
+ is CD233.</td>
+ </tr>
+ <tr>
+ <td>citation</td>
+ <td><code>
+ citation:("intracellular structural proteins") <br />
+ citation:(author:ashburner journal:nature) citation:9169874
+ </code></td>
+ <td>Lists all entries with a literature citation:
+ <ul>
+ <li>containing the phrase "intracellular structural
+ proteins" in either title or abstract</li>
+ <li>co-authored by Michael Ashburner and published in
+ Nature</li>
+ <li>with the PubMed identifier 9169874</li>
+ </ul>
+ </td>
+ </tr>
+ <tr>
+ <td>cluster</td>
+ <td><code> cluster:UniRef90_A5YMT3 </code></td>
+ <td>Lists all entries in the UniRef 90% identity cluster
+ whose representative sequence is UniProtKB entry A5YMT3.</td>
+ </tr>
+ <tr>
+ <td>count</td>
+ <td><code>
+ annotation:(type:transmem count:5)<br />
+ annotation:(type:transmem count:[5 TO *])<br />
+ annotation:(type:cofactor count:[3 TO *])
+ </code></td>
+ <td>Lists all entries with:
+ <ul>
+ <li>exactly 5 transmembrane regions</li>
+ <li>5 or more transmembrane regions</li>
+ <li>3 or more Cofactor comments</li>
+ </ul>
+ </td>
+ </tr>
+ <tr>
+ <td>created</td>
+ <td><code>
+ created:[20121001 TO *]<br /> reviewed:yes AND
+ created:[current TO *]
+ </code></td>
+ <td>Lists all entries created since October 1st 2012.<br />
Lists all new UniProtKB/Swiss-Prot entries in the last release.
- </td>
- </tr>
- <tr>
- <td>database</td>
- <td>
- <code>
- database:(type:pfam)
- <br />
- database:(type:pdb 1aut)
- </code>
- </td>
- <td>
- Lists all entries with:
- <ul>
- <li>a cross-reference to the Pfam database</li>
- <li>a cross-reference to the PDB database entry 1aut</li>
- </ul>
-
- </td>
- </tr>
- <tr>
- <td>domain</td>
- <td>
- <code>
- domain:VWFA
- </code>
- </td>
- <td>
- Lists all entries with a Von Willebrand factor type A domain described
- in the 'Family and Domains' section.
- </td>
- </tr>
- <tr>
- <td>ec</td>
- <td>
- <code>
- ec:3.2.1.23
- </code>
- </td>
- <td>
- Lists all beta-galactosidases.
- </td>
- </tr>
- <tr>
- <td>evidence</td>
- <td>
- <code>
- annotation:(type:signal evidence:ECO_0000269)<br />
- (type:mod_res phosphoserine evidence:ECO_0000269)<br />
- annotation:(type:function AND evidence:ECO_0000255)
- </code>
- </td>
- <td>Lists all entries with:
- <ul>
- <li>a signal sequence whose positions have been experimentally proven</li>
- <li>experimentally proven phosphoserine sites</li>
- <li>a function manually asserted according to rules</li>
- </ul>
- </td>
- </tr>
- <tr>
- <td>family</td>
- <td>
- <code>
- family:serpin
- </code>
- </td>
- <td>
- Lists all entries belonging to the Serpin family of proteins.
- </td>
- </tr>
- <tr>
- <td>fragment</td>
- <td>
- <code>
- fragment:yes
- </code>
- </td>
- <td>
- Lists all entries with an incomplete sequence.
- </td>
- </tr>
+ </td>
+ </tr>
+ <tr>
+ <td>database</td>
+ <td><code>
+ database:(type:pfam) <br /> database:(type:pdb 1aut)
+ </code></td>
+ <td>Lists all entries with:
+ <ul>
+ <li>a cross-reference to the Pfam database</li>
+ <li>a cross-reference to the PDB database entry 1aut</li>
+ </ul>
- <tr>
- <td>gene</td>
- <td>
- <code>
- gene:HSPC233
- </code>
- </td>
- <td>
- Lists all entries for proteins encoded by gene HSPC233.
- </td>
- </tr>
- <tr>
- <td>go</td>
- <td>
- <code>
- go:cytoskeleton
- <br />
- go:0015629
- </code>
- </td>
- <td>
- Lists all entries associated with:
- <ul>
- <li>a GO term containing the word "cytoskeleton"</li>
- <li>the GO term Actin cytoskeleton and any subclasses</li>
- </ul>
- </td>
- </tr>
- <tr>
- <td>host</td>
- <td>
- <code>
- host:mouse
- <br />
- host:10090
- <br />
- host:40674
- </code>
- </td>
- <td>
- Lists all entries for viruses infecting:
- <ul>
- <li>organisms with a name containing the word "mouse"</li>
- <li>Mus musculus (Mouse)</li>
- <li>all mammals (all taxa classified under the taxonomy node for Mammalia)</li>
- </ul>
- </td>
- </tr>
- <tr>
- <td>id</td>
- <td>
- <code>id:P00750</code>
- </td>
- <td>
- Returns the entry with the primary
- accession number P00750.
- </td>
- </tr>
- <tr>
- <td>inn</td>
- <td>
- <code>
- inn:Anakinra
- </code>
- </td>
- <td>
- Lists all entries whose "International Nonproprietary Name" is Anakinra.
- </td>
- </tr>
- <tr>
- <td>interactor</td>
- <td>
- <code>
- interactor:P00520
- </code>
- </td>
- <td>
- Lists all entries describing interactions with the protein described by
- entry P00520.
- </td>
- </tr>
- <tr>
- <td>keyword</td>
- <td>
- <code>
- keyword:toxin
- </code>
- </td>
- <td>
- Lists all entries associated with the keyword Toxin.
- </td>
- </tr>
- <tr>
- <td>length</td>
- <td>
- <code>
- length:[500 TO 700]
- </code>
- </td>
- <td>
- Lists all entries describing sequences of length between 500 and 700 residues.
- </td>
- </tr>
- <tr>
- <td>lineage</td>
- <td />
- <td>
- This field is a synonym for the field <code>taxonomy</code>.
- </td>
- </tr>
- <tr>
- <td>mass</td>
- <td>
- <code>
- mass:[500000 TO *]
- </code>
- </td>
- <td>
- Lists all entries describing sequences with a mass of at least 500,000 Da.
- </td>
- </tr>
- <tr>
- <td>method</td>
- <td>
- <code>
- method:maldi
- <br />
- method:xray
- </code>
- </td>
- <td>
- Lists all entries for proteins identified by: matrix-assisted laser
- desorption/ionization (MALDI), crystallography (X-Ray). The
- <code>method</code> field searches names of physico-chemical
- identification methods in the 'Biophysicochemical properties' subsection of the 'Function' section, the 'Publications' and
- 'Cross-references' sections.
- </td>
- </tr>
- <tr>
- <td>mnemonic</td>
- <td>
- <code>
- mnemonic:ATP6_HUMAN
- </code>
- </td>
- <td>
- Lists all entries with entry name (ID) ATP6_HUMAN. Searches also
- obsolete entry names.
- </td>
- </tr>
- <tr>
- <td>modified</td>
- <td>
- <code>
- modified:[20120101 TO 20120301]<br />
- reviewed:yes AND modified:[current TO *]
- </code>
- </td>
- <td>
- Lists all entries that were last modified between January and March 2012.<br />
- Lists all UniProtKB/Swiss-Prot entries modified in the last release.
- </td>
- </tr>
- <tr>
- <td>name</td>
- <td>
- <code>
- name:"prion protein"
- </code>
- </td>
- <td>
- Lists all entries for prion proteins.
- </td>
- </tr>
- <tr>
- <td>organelle</td>
- <td>
- <code>
- organelle:Mitochondrion
- </code>
- </td>
- <td>
- Lists all entries for proteins encoded by a gene of the mitochondrial
- chromosome.
- </td>
- </tr>
- <tr>
- <td>organism</td>
- <td>
- <code>
- organism:"Ovis aries"
- <br />
- organism:9940
- <br />
- organism:sheep
- <br />
- </code>
- </td>
- <td>
- Lists all entries for proteins expressed in sheep (first 2 examples) and
- organisms whose name contains the term "sheep".
- </td>
- </tr>
-
- <tr>
- <td>plasmid</td>
- <td>
- <code>
- plasmid:ColE1
- </code>
- </td>
- <td>
- Lists all entries for proteins encoded by a gene of plasmid ColE1.
- </td>
- </tr>
- <tr>
- <td>proteome</td>
- <td>
- <code>
- proteome:UP000005640
- </code>
- </td>
- <td>
- Lists all entries from the human proteome.
- </td>
- </tr>
- <tr>
- <td>proteomecomponent</td>
- <td>
- <code>
- proteomecomponent:"chromosome 1" and organism:9606
- </code>
- </td>
- <td>
- Lists all entries from the human chromosome 1.
- </td>
- </tr>
- <tr>
- <td>replaces</td>
- <td>
- <code>
- replaces:P02023
- </code>
- </td>
- <td>
- Lists all entries that were created from a merge with entry P02023.
- </td>
- </tr>
- <tr>
- <td>reviewed</td>
- <td>
- <code>
- reviewed:yes
- </code>
- </td>
- <td>
- Lists all UniProtKB/Swiss-Prot entries.
- </td>
- </tr>
- <tr>
- <td>scope</td>
- <td>
- <code>
- scope:mutagenesis
- </code>
- </td>
- <td>
- Lists all entries containing a reference that was used to gather
- information about mutagenesis.
- </td>
- </tr>
- <tr>
- <td>sequence</td>
- <td>
- <code>
- sequence:P05067-9
- </code>
- </td>
- <td>
- Lists all entries containing a link to isoform 9 of the sequence
- described in entry P05067. Allows searching by specific sequence
- identifier.
- </td>
- </tr>
- <tr>
- <td>sequence_modified</td>
- <td>
- <code>
- sequence_modified:[20120101 TO 20120301]<br />
- reviewed:yes AND sequence_modified:[current TO *]
- </code>
- </td>
- <td>
- Lists all entries whose sequences were last modified between January and March 2012.<br />
- Lists all UniProtKB/Swiss-Prot entries whose sequences were modified in the last release.
- </td>
- </tr>
- <tr>
- <td>source</td>
- <td>
- <code>
- source:intact
- </code>
- </td>
- <td>
- Lists all entries containing a GO term whose annotation source is the
- IntAct database.
- </td>
- </tr>
- <tr>
- <td>strain</td>
- <td>
- <code>
- strain:wistar
- </code>
- </td>
- <td>
- Lists all entries containing a reference relevant to strain wistar.
- </td>
- </tr>
- <tr>
- <td>taxonomy</td>
- <td>
- <code>
- taxonomy:40674
- </code>
- </td>
- <td>
- Lists all entries for proteins expressed in Mammals. This field is used to retrieve
- entries for all organisms classified below a given taxonomic node taxonomy classification).
- </td>
- </tr>
- <tr>
- <td>tissue</td>
- <td>
- <code>
- tissue:liver
- </code>
- </td>
- <td>
- Lists all entries containing a reference describing the protein sequence
- obtained from a clone isolated from liver.
- </td>
- </tr>
- <tr>
- <td>web</td>
- <td>
- <code>
- web:wikipedia
- </code>
- </td>
- <td>
- Lists all entries for proteins that are described in Wikipedia.
- </td>
- </tr>
-</table>
+ </td>
+ </tr>
+ <tr>
+ <td>domain</td>
+ <td><code> domain:VWFA </code></td>
+ <td>Lists all entries with a Von Willebrand factor type A
+ domain described in the 'Family and Domains' section.</td>
+ </tr>
+ <tr>
+ <td>ec</td>
+ <td><code> ec:3.2.1.23 </code></td>
+ <td>Lists all beta-galactosidases.</td>
+ </tr>
+ <tr>
+ <td>evidence</td>
+ <td><code>
+ annotation:(type:signal evidence:ECO_0000269)<br />
+ (type:mod_res phosphoserine evidence:ECO_0000269)<br />
+ annotation:(type:function AND evidence:ECO_0000255)
+ </code></td>
+ <td>Lists all entries with:
+ <ul>
+ <li>a signal sequence whose positions have been
+ experimentally proven</li>
+ <li>experimentally proven phosphoserine sites</li>
+ <li>a function manually asserted according to rules</li>
+ </ul>
+ </td>
+ </tr>
+ <tr>
+ <td>family</td>
+ <td><code> family:serpin </code></td>
+ <td>Lists all entries belonging to the Serpin family of
+ proteins.</td>
+ </tr>
+ <tr>
+ <td>fragment</td>
+ <td><code> fragment:yes </code></td>
+ <td>Lists all entries with an incomplete sequence.</td>
+ </tr>
+
+ <tr>
+ <td>gene</td>
+ <td><code> gene:HSPC233 </code></td>
+ <td>Lists all entries for proteins encoded by gene HSPC233.</td>
+ </tr>
+ <tr>
+ <td>go</td>
+ <td><code>
+ go:cytoskeleton <br /> go:0015629
+ </code></td>
+ <td>Lists all entries associated with:
+ <ul>
+ <li>a GO term containing the word "cytoskeleton"</li>
+ <li>the GO term Actin cytoskeleton and any subclasses</li>
+ </ul>
+ </td>
+ </tr>
+ <tr>
+ <td>host</td>
+ <td><code>
+ host:mouse <br /> host:10090 <br /> host:40674
+ </code></td>
+ <td>Lists all entries for viruses infecting:
+ <ul>
+ <li>organisms with a name containing the word "mouse"</li>
+ <li>Mus musculus (Mouse)</li>
+ <li>all mammals (all taxa classified under the taxonomy
+ node for Mammalia)</li>
+ </ul>
+ </td>
+ </tr>
+ <tr>
+ <td>id</td>
+ <td><code>id:P00750</code></td>
+ <td>Returns the entry with the primary accession number
+ P00750.</td>
+ </tr>
+ <tr>
+ <td>inn</td>
+ <td><code> inn:Anakinra </code></td>
+ <td>Lists all entries whose "International Nonproprietary
+ Name" is Anakinra.</td>
+ </tr>
+ <tr>
+ <td>interactor</td>
+ <td><code> interactor:P00520 </code></td>
+ <td>Lists all entries describing interactions with the
+ protein described by entry P00520.</td>
+ </tr>
+ <tr>
+ <td>keyword</td>
+ <td><code> keyword:toxin </code></td>
+ <td>Lists all entries associated with the keyword Toxin.</td>
+ </tr>
+ <tr>
+ <td>length</td>
+ <td><code> length:[500 TO 700] </code></td>
+ <td>Lists all entries describing sequences of length between
+ 500 and 700 residues.</td>
+ </tr>
+ <tr>
+ <td>lineage</td>
+ <td />
+ <td>This field is a synonym for the field <code>taxonomy</code>.
+ </td>
+ </tr>
+ <tr>
+ <td>mass</td>
+ <td><code> mass:[500000 TO *] </code></td>
+ <td>Lists all entries describing sequences with a mass of at
+ least 500,000 Da.</td>
+ </tr>
+ <tr>
+ <td>method</td>
+ <td><code>
+ method:maldi <br /> method:xray
+ </code></td>
+ <td>Lists all entries for proteins identified by:
+ matrix-assisted laser desorption/ionization (MALDI),
+ crystallography (X-Ray). The <code>method</code> field searches
+ names of physico-chemical identification methods in the
+ 'Biophysicochemical properties' subsection of the 'Function'
+ section, the 'Publications' and 'Cross-references' sections.
+ </td>
+ </tr>
+ <tr>
+ <td>mnemonic</td>
+ <td><code> mnemonic:ATP6_HUMAN </code></td>
+ <td>Lists all entries with entry name (ID) ATP6_HUMAN.
+ Searches also obsolete entry names.</td>
+ </tr>
+ <tr>
+ <td>modified</td>
+ <td><code>
+ modified:[20120101 TO 20120301]<br /> reviewed:yes AND
+ modified:[current TO *]
+ </code></td>
+ <td>Lists all entries that were last modified between January
+ and March 2012.<br /> Lists all UniProtKB/Swiss-Prot entries
+ modified in the last release.
+ </td>
+ </tr>
+ <tr>
+ <td>name</td>
+ <td><code> name:"prion protein" </code></td>
+ <td>Lists all entries for prion proteins.</td>
+ </tr>
+ <tr>
+ <td>organelle</td>
+ <td><code> organelle:Mitochondrion </code></td>
+ <td>Lists all entries for proteins encoded by a gene of the
+ mitochondrial chromosome.</td>
+ </tr>
+ <tr>
+ <td>organism</td>
+ <td><code>
+ organism:"Ovis aries" <br /> organism:9940 <br />
+ organism:sheep <br />
+ </code></td>
+ <td>Lists all entries for proteins expressed in sheep (first
+ 2 examples) and organisms whose name contains the term "sheep".
+ </td>
+ </tr>
+
+ <tr>
+ <td>plasmid</td>
+ <td><code> plasmid:ColE1 </code></td>
+ <td>Lists all entries for proteins encoded by a gene of
+ plasmid ColE1.</td>
+ </tr>
+ <tr>
+ <td>proteome</td>
+ <td><code> proteome:UP000005640 </code></td>
+ <td>Lists all entries from the human proteome.</td>
+ </tr>
+ <tr>
+ <td>proteomecomponent</td>
+ <td><code> proteomecomponent:"chromosome 1" and
+ organism:9606 </code></td>
+ <td>Lists all entries from the human chromosome 1.</td>
+ </tr>
+ <tr>
+ <td>replaces</td>
+ <td><code> replaces:P02023 </code></td>
+ <td>Lists all entries that were created from a merge with
+ entry P02023.</td>
+ </tr>
+ <tr>
+ <td>reviewed</td>
+ <td><code> reviewed:yes </code></td>
+ <td>Lists all UniProtKB/Swiss-Prot entries.</td>
+ </tr>
+ <tr>
+ <td>scope</td>
+ <td><code> scope:mutagenesis </code></td>
+ <td>Lists all entries containing a reference that was used to
+ gather information about mutagenesis.</td>
+ </tr>
+ <tr>
+ <td>sequence</td>
+ <td><code> sequence:P05067-9 </code></td>
+ <td>Lists all entries containing a link to isoform 9 of the
+ sequence described in entry P05067. Allows searching by specific
+ sequence identifier.</td>
+ </tr>
+ <tr>
+ <td>sequence_modified</td>
+ <td><code>
+ sequence_modified:[20120101 TO 20120301]<br /> reviewed:yes
+ AND sequence_modified:[current TO *]
+ </code></td>
+ <td>Lists all entries whose sequences were last modified
+ between January and March 2012.<br /> Lists all
+ UniProtKB/Swiss-Prot entries whose sequences were modified in
+ the last release.
+ </td>
+ </tr>
+ <tr>
+ <td>source</td>
+ <td><code> source:intact </code></td>
+ <td>Lists all entries containing a GO term whose annotation
+ source is the IntAct database.</td>
+ </tr>
+ <tr>
+ <td>strain</td>
+ <td><code> strain:wistar </code></td>
+ <td>Lists all entries containing a reference relevant to
+ strain wistar.</td>
+ </tr>
+ <tr>
+ <td>taxonomy</td>
+ <td><code> taxonomy:40674 </code></td>
+ <td>Lists all entries for proteins expressed in Mammals. This
+ field is used to retrieve entries for all organisms classified
+ below a given taxonomic node taxonomy classification).</td>
+ </tr>
+ <tr>
+ <td>tissue</td>
+ <td><code> tissue:liver </code></td>
+ <td>Lists all entries containing a reference describing the
+ protein sequence obtained from a clone isolated from liver.</td>
+ </tr>
+ <tr>
+ <td>web</td>
+ <td><code> web:wikipedia </code></td>
+ <td>Lists all entries for proteins that are described in
+ Wikipedia.</td>
+ </tr>
+ </table>
</body>
</html>
\ No newline at end of file
mnt_mouse).<br />Hitting Return or OK will automatically fetch
those IDs, like the default Sequence Fetcher interface.</li>
- <li><strong><a name="text-search">Complex queries with the UniProt query
- Syntax</a></strong> The text box also allows complex queries to be entered.
- The table below provides a brief overview of the supported syntax
- (see <a href="uniprotqueryfields.html">query fields for
- UniProtKB</a>):
+ <li><strong><a name="text-search">Complex queries
+ with the UniProt query Syntax</a></strong> The text box also allows complex
+ queries to be entered. The table below provides a brief overview
+ of the supported syntax (see <a href="uniprotqueryfields.html">query
+ fields for UniProtKB</a>):
<table border="1" width="95%">
<tr>
<td><code>human antigen</code></td>
<strong>The VARNA RNA Viewer</strong>
</p>
<p>
- <a href="http://varna.lri.fr">VARNA</a> was integrated
- into Jalview 2.8 to allow interactive viewing of RNA secondary
- structure annotation. It is opened by selecting the <strong>"Structure→View
+ <a href="http://varna.lri.fr">VARNA</a> was integrated into Jalview
+ 2.8 to allow interactive viewing of RNA secondary structure
+ annotation. It is opened by selecting the <strong>"Structure→View
Structure:"</strong> option in the <a href="../menus/popupMenu.html">sequence
id pop-up menu</a> (if you can't see this, then no RNA structure is
associated with your sequence or alignment). In the pop-up menu all
<p>
VARNA is a very powerful RNA viewer on its own. Only the essentials
have been described here - the interested reader is referred to <a
- href="http://varna.lri.fr/index.php?page=manual&css=varna"
- >VARNA's own comprehensive online documentation</a>.
+ href="http://varna.lri.fr/index.php?page=manual&css=varna">VARNA's
+ own comprehensive online documentation</a>.
</p>
</body>
</html>
</ul>
</li>
<li><strong>Selecting Structures</strong><br />You can select
- the structures that you want to open and view by selecting them with
- the mouse and keyboard.<br />By default, if structures were
+ the structures that you want to open and view by selecting them
+ with the mouse and keyboard.<br />By default, if structures were
discovered, then some will already be selected according to the
criteria shown in the drop-down menu. The default criteria is
'highest resolution', simply choose another to pick structures in
dialog box.</li>
</ul></li>
<li><strong>To view selected structures, click the <strong>"View"</strong>
- button.</strong><br />
+ button.
+ </strong><br />
<ul>
<li>Additional structure data will be downloaded with the
EMBL-EBI's dbfetch service</li>
retrieved with this service are derived directly from the PDB 3D
structure data, which can be viewed in the same way above. Secondary
structure and temperature factor annotation can also be added. <br />
-
- <br>Jalview
- will also read PDB files directly - either in PDB format, or <a
- href="mmcif.html">mmCIF</a>. Simply load in the file as you would
- an alignment file. The sequences of any protein or nucleotide chains
- will be extracted from the file and viewed in the alignment window.
+
+ <br>Jalview will also read PDB files directly - either in PDB
+ format, or <a href="mmcif.html">mmCIF</a>. Simply load in the file
+ as you would an alignment file. The sequences of any protein or
+ nucleotide chains will be extracted from the file and viewed in the
+ alignment window.
</p>
<p>
Features"</strong> menu item and the <a href="featuresettings.html">Feature
Settings dialog box</a>.
</p>
- <br/>
+ <br />
<hr>
<p>
<strong>Switching between mmCIF and PDB format for
tab in the <strong>Tools→Preferences</strong> dialog allow the
processing of structure data to be disabled, or selectively enabled.
For more information, take a look at the <a
- href="preferences.html#structure"
- >documentation for the structure panel</a>.
+ href="preferences.html#structure">documentation for the
+ structure panel</a>.
</p>
<p>
<em>The display of secondary structure data was introduced in
Jalview 2.8.2, and is made possible by Jalview's use of <a
- href="jmol.html"
- >Jmol's DSSP implementation</a>, based on the original <a
- href="http://www.ncbi.nlm.nih.gov/pubmed/6667333"
- >Kabsch and Sander algorithm</a> ported by <a
- href="http://swift.cmbi.ru.nl/gv/dssp/"
- >Robbie P. Joosten and colleagues</a>, and a client for <a
- href="https://github.com/fjossinet/PyRNA"
- >Fabrice Jossinet's pyRNA services</a> that was developed by Anne
- Menard, Jim Procter and Yann Ponty as part of the Jalview Summer
- of Code 2012.
+ href="jmol.html">Jmol's DSSP implementation</a>, based on the
+ original <a href="http://www.ncbi.nlm.nih.gov/pubmed/6667333">Kabsch
+ and Sander algorithm</a> ported by <a
+ href="http://swift.cmbi.ru.nl/gv/dssp/">Robbie P. Joosten
+ and colleagues</a>, and a client for <a
+ href="https://github.com/fjossinet/PyRNA">Fabrice
+ Jossinet's pyRNA services</a> that was developed by Anne Menard, Jim
+ Procter and Yann Ponty as part of the Jalview Summer of Code 2012.
</em>
</p>
</body>
<body>
<p>
<strong>Extending Jalview with Groovy - A customisable
- feature counter</strong><br />
- <br />The groovy script below shows how to add a new calculation
- track to a Jalview alignment window.</p><p>As currently written, it will
- add two tracks to a protein alignment view which count Pfam features
- in each column, and ones where a charge residue also occur.</p><p>To try
- it for yourself:</p><ol><li>Copy and paste it into the groovy script
- console</li><li>Load the example Feredoxin project (the one that opens
- by default when you first launched Jalview)</li><li>Select <strong>Calculations→Execute
- Groovy Script</strong> from the alignment window's menu bar to run the script on
- the current view.</li></ol>
+ feature counter</strong><br /> <br />The groovy script below shows how to
+ add a new calculation track to a Jalview alignment window.
+ </p>
+ <p>As currently written, it will add two tracks to a protein
+ alignment view which count Pfam features in each column, and ones
+ where a charge residue also occur.</p>
+ <p>To try it for yourself:</p>
+ <ol>
+ <li>Copy and paste it into the groovy script console</li>
+ <li>Load the example Feredoxin project (the one that opens by
+ default when you first launched Jalview)</li>
+ <li>Select <strong>Calculations→Execute Groovy
+ Script</strong> from the alignment window's menu bar to run the script on
+ the current view.
+ </li>
+ </ol>
<em><a
- href="http://www.jalview.org/examples/groovy/featureCounter.groovy">http://www.jalview.org/examples/groovy/featureCounter.groovy</a> - rendered with <a href="http://hilite.me">hilite.me</a></em>
+ href="http://www.jalview.org/examples/groovy/featureCounter.groovy">http://www.jalview.org/examples/groovy/featureCounter.groovy</a>
+ - rendered with <a href="http://hilite.me">hilite.me</a></em>
<!-- HTML generated using hilite.me -->
<div
style="background: #ffffff; overflow: auto; width: auto; border: solid gray; border-width: .1em .1em .1em .8em; padding: .2em .6em;">
<p>
For more information, you might also want to take a look at the
documentation section of the Jalview website (<a
- href="http://www-test.jalview.org/about/documentation"
- >http://www.jalview.org/about/documentation</a>).
+ href="http://www-test.jalview.org/about/documentation">http://www.jalview.org/about/documentation</a>).
</p>
<p>
If you are using the Jalview Desktop application and are looking for
google the online version of these pages. If you don't find what you
are looking for, or want to report a bug or make a feature request,
then get in contact over at <a
- href="http://www.jalview.org/community"
- >http://www.jalview.org/community</a>
+ href="http://www.jalview.org/community">http://www.jalview.org/community</a>
</p>
<p>
<strong>25</strong> (9) 1189-1191 doi: 10.1093/bioinformatics/btp033
</p>
<p>
- <strong>The Jalview Authors</strong><br /> The following people have
- contributed to Jalview's development:
+ <strong>The Jalview Authors</strong><br /> The following people
+ have contributed to Jalview's development:
<ul>
<li>Jalview 1
<ul>
<body>
<p>
<strong>Exporting alignments as graphics and lineart<a
- name="export"
- ></a></strong>
+ name="export"></a></strong>
</p>
<p>
The alignment view can be printed using <strong>File→Print</strong>,
menu</a>.
</p>
<img src="seqreport.gif"
- alt="Sequence Annotation is displayed as HTML in a report window"
- />
+ alt="Sequence Annotation is displayed as HTML in a report window" />
<p>
<strong>Copying and pasting annotation to other programs</strong><br>
The <strong>File→Save</strong> option in the sequence
<tr>
<td width="17%">JSON</td>
<td width="60%">Data starts with '{' <br>Data ends with
- '}' <br>
- <br>See <a href="../features/bioJsonFormat.html">BioJSON</a>
- for more infomation about the Jalview JSON format <br></td>
+ '}' <br> <br>See <a
+ href="../features/bioJsonFormat.html">BioJSON</a> for more
+ infomation about the Jalview JSON format <br></td>
<td width="23%">.json</td>
</tr>
</p>
<p>
Jalview can also read Jalview specific files for <a
- href="../features/featuresFormat.html"
- >sequence features</a> and <a
- href="../features/annotationsFormat.html"
- >alignment annotation</a>.
+ href="../features/featuresFormat.html">sequence features</a>
+ and <a href="../features/annotationsFormat.html">alignment
+ annotation</a>.
</p>
<p>
<strong>Output</strong>
</p>
<p>
The homology modelling program, <a
- href="http://salilab.org/modeller/"
- >Modeller</a> uses a special form of the PIR format where information
- about sequence numbering and chain codes are written into the
- 'description' line between the PIR protein tag and the protein
- alignment entry:
+ href="http://salilab.org/modeller/">Modeller</a> uses a
+ special form of the PIR format where information about sequence
+ numbering and chain codes are written into the 'description' line
+ between the PIR protein tag and the protein alignment entry:
</p>
<pre>>P1;Q93Z60_ARATH
sequence:Q93Z60_ARATH:1:.:118:.:.
no information is lost if this parsing process fails.</p>
<p>
The 'Modeller Output' flag in the 'Output' tab of the Jalview <a
- href="../features/preferences.html"
- >Preferences dialog box</a> controls whether Jalview will also output
- MODELLER style PIR files. In this case, any existing 'non-modeller
- PIR' header information in the description string of an alignment is
- appended to an automatically generated modeller description line for
- that sequence.
+ href="../features/preferences.html">Preferences dialog
+ box</a> controls whether Jalview will also output MODELLER style PIR
+ files. In this case, any existing 'non-modeller PIR' header
+ information in the description string of an alignment is appended to
+ an automatically generated modeller description line for that
+ sequence.
</p>
<p>The general format used for generating the Modeller/PIR
sequence description line is shown below :
T-COFFEE score files like the <a href="#tcoffeeeg">one below</a> can
be displayed on the alignment using the <strong><em>Colours→T-COFFEE
Scores</em></strong> or <strong><em>Colour → <a
- href="../colourSchemes/annotationColouring.html"
- >Colour by Annotation</a></em></strong> options.
+ href="../colourSchemes/annotationColouring.html">Colour
+ by Annotation</a></em></strong> options.
</p>
<img src="../colourSchemes/colbytcoffee.png" />
<p>
Jalview has two distinct modes of keyboard operation - in 'Normal'
mode, single keystrokes (including those shown next to menu items)
provide short cuts to common commands. In <a
- href="features/cursorMode.html"
- >'Cursor'</a> mode (enabled by <em>F2</em>), some of these are
- disabled and more complex 'Compound Keystrokes' can be entered to
- perform precise navigation, selection and editing operations.
+ href="features/cursorMode.html">'Cursor'</a> mode (enabled by
+ <em>F2</em>), some of these are disabled and more complex 'Compound
+ Keystrokes' can be entered to perform precise navigation, selection
+ and editing operations.
</p>
<table border="1">
<tr>
<td>Cursor</td>
<td>Move cursor to a particular column (<strong><em>p1</em></strong>)
and row (<strong><em>p2</em></strong>) in the alignment.<br>
- <em>e.g. '5,6<Return>' moves the cursor to the 5th
+ <em>e.g. '5,6<Return>' moves the cursor to the 5th
column in the 6th sequence.</em></td>
</tr>
<tr>
<td><strong><em>[p]</em><br>Space</strong></td>
<td>Cursor</td>
<td>Inserts one (or optionally <strong><em>p</em></strong>)
- gaps at the current position.<br>
- <em>Hold down Control or Shift to insert gaps over a sequence
- group</em></td>
+ gaps at the current position.<br> <em>Hold down
+ Control or Shift to insert gaps over a sequence group</em></td>
</tr>
<tr>
<td><strong><em>[p]</em><br>Delete<br></strong></td>
<td>Cursor</td>
<td>Removes one (or optionally <strong><em>p</em></strong>)
- gaps at the cursor position.<br>
- <em>Hold down Control or Shift to insert gaps over a sequence
- group</em></td>
+ gaps at the cursor position.<br> <em>Hold down Control
+ or Shift to insert gaps over a sequence group</em></td>
</tr>
<tr>
<td><strong><em>[p]</em><br>Backspace<br></strong></td>
<td>Cursor</td>
<td>Removes one (or optionally <strong><em>p</em></strong>)
- gaps at the cursor position.<br>
- <em>Hold down Control or Shift to insert gaps over a sequence
- group</em></td>
+ gaps at the cursor position.<br> <em>Hold down Control
+ or Shift to insert gaps over a sequence group</em></td>
</tr>
</table>
<p> </p>
file. You can obtain a JNLP file with modified memory settings
from our service with the following link (replace 2G with
desired memory in G or M):<br /> <a
- href="http://www.jalview.org/services/launchApp?jvm-max-heap=2G"
- >http://www.jalview.org/services/launchApp?jvm-max-heap=2G</a>
+ href="http://www.jalview.org/services/launchApp?jvm-max-heap=2G">http://www.jalview.org/services/launchApp?jvm-max-heap=2G</a>
</p>
<p>
Alternatively, if you want to create your own JNLP file then
please download the latest JNLP file from <a
- href="http://www.jalview.org/webstart/jalview.jnlp"
- >http://www.jalview.org/webstart/jalview.jnlp</a> and modify the
- max-heap-size parameter for the j2se tag in the
+ href="http://www.jalview.org/webstart/jalview.jnlp">http://www.jalview.org/webstart/jalview.jnlp</a>
+ and modify the max-heap-size parameter for the j2se tag in the
<resources> element. e.g.
<pre>
<j2se version="1.7+" initial-heap-size="500M" max-heap-size="1000M"/>
The lines you need to change are in the <em>Info.plist</em>
file inside the <em>Jalview.app/Contents</em> directory
(which is where the installAnywhere installation was made) :
+
<pre>
<key&ht;VMOptions</key&ht;
</em></li>
<li><strong>Load Features / Annotations<br>
</strong><em>Load files describing precalculated <a
- href="../features/featuresFormat.html"
- >sequence features</a> or <a
- href="../features/annotationsFormat.html"
- >alignment annotations</a>.
+ href="../features/featuresFormat.html">sequence
+ features</a> or <a href="../features/annotationsFormat.html">alignment
+ annotations</a>.
</em></li>
<li><strong>Close (Control W)</strong><br> <em>Close
the alignment window. Make sure you have saved your
</strong><em>All columns which only contain gap characters
("-", ".") will be deleted.<br> You
may set the default gap character in <a
- href="../features/preferences.html"
- >preferences</a>.
+ href="../features/preferences.html">preferences</a>.
</em></li>
<li><strong>Remove All Gaps (Control Shift E)</strong><br>
<em>Gap characters ("-", ".") will be
deleted from the selected area of the alignment. If no
selection is made, ALL the gaps in the alignment will be
removed.<br> You may set the default gap character in <a
- href="../features/preferences.html"
- >preferences</a>.
+ href="../features/preferences.html">preferences</a>.
</em></li>
<li><strong>Remove Redundancy (Control D)<br>
</strong><em>Selecting this option brings up a window asking you to
with alignment analysis programs which require 'properly
aligned sequences' to be all the same length.<br> You
may set the default for <strong>Pad Gaps</strong> in the <a
- href="../features/preferences.html"
- >preferences</a>.
+ href="../features/preferences.html">preferences</a>.
</em></li>
</ul></li>
<li><strong>Select</strong>
<strong>WARNING</strong>: This cannot be undone.
</em></li>
<li><strong><a
- href="../features/columnFilterByAnnotation.html"
- >Select/Hide Columns by Annotation</a></strong> <br /> <em>Select
- or Hide columns in the alignment according to secondary
- structure, labels and values shown in alignment annotation
- rows. </em></li>
+ href="../features/columnFilterByAnnotation.html">Select/Hide
+ Columns by Annotation</a></strong> <br /> <em>Select or Hide
+ columns in the alignment according to secondary structure,
+ labels and values shown in alignment annotation rows. </em></li>
</ul></li>
<li><strong>View</strong>
<ul>
<li><strong>Show Sequence Features</strong><br> <em>Show
or hide sequence features on this alignment.</em></li>
<li><strong><a
- href="../features/featuresettings.html"
- >Sequence Feature Settings...</a> </strong><em><br> <em>Opens
- the Sequence Feature Settings dialog box to control the
- colour and display of sequence features on the alignment,
- and configure and retrieve features from DAS annotation
+ href="../features/featuresettings.html">Sequence
+ Feature Settings...</a> </strong><em><br> <em>Opens the
+ Sequence Feature Settings dialog box to control the colour
+ and display of sequence features on the alignment, and
+ configure and retrieve features from DAS annotation
servers.</em></li>
<li><strong>Sequence ID Tooltip</strong><em>
(application only) <br>This submenu's options allow the
rendering. </em></li>
<li><strong>Wrap<br>
</strong><em>When ticked, the alignment display is "<a
- href="../features/wrap.html"
- >wrapped</a>" to the width of the alignment window. This is
- useful if your alignment has only a few sequences to view
- its full width at once.
+ href="../features/wrap.html">wrapped</a>" to
+ the width of the alignment window. This is useful if your
+ alignment has only a few sequences to view its full width at
+ once.
</em><br> Additional options for display of sequence numbering
and scales are also visible in wrapped layout mode:<br>
<ul>
- <li><strong>Scale Above</strong><br>
- <em> Show the alignment column position scale.</em></li>
- <li><strong>Scale Left</strong><br>
- <em> Show the sequence position for the first aligned
- residue in each row in the left column of the alignment.</em></li>
- <li><strong>Scale Right</strong><br>
- <em> Show the sequence position for the last aligned
- residue in each row in the right-most column of the
- alignment.</em></li>
+ <li><strong>Scale Above</strong><br> <em>
+ Show the alignment column position scale.</em></li>
+ <li><strong>Scale Left</strong><br> <em> Show
+ the sequence position for the first aligned residue in
+ each row in the left column of the alignment.</em></li>
+ <li><strong>Scale Right</strong><br> <em>
+ Show the sequence position for the last aligned residue
+ in each row in the right-most column of the alignment.</em></li>
<li><strong>Show Sequence Limits<br>
</strong><em>If this box is selected the sequence name will have
the start and end position of the sequence appended to
colour will be applied to all currently defined groups.<br>
</em></li>
<li><strong><a
- href="../colourSchemes/textcolour.html"
- >Colour Text...</a> </strong><em><br> Opens the Colour Text
- dialog box to set a different text colour for light and dark
- background, and the intensity threshold for transition between
- them. </em></li>
+ href="../colourSchemes/textcolour.html">Colour
+ Text...</a> </strong><em><br> Opens the Colour Text dialog box
+ to set a different text colour for light and dark background,
+ and the intensity threshold for transition between them. </em></li>
<li>Colour Scheme options: <strong>None, ClustalX,
Blosum62 Score, Percentage Identity, Zappo, Taylor,
Hydrophobicity, Helix Propensity, Strand Propensity, Turn
<li><strong>By Annotation</strong><br> <em>Colours
the alignment on a per-column value from a specified
annotation. See <a
- href="../colourSchemes/annotationColouring.html"
- >Annotation Colouring</a>.
+ href="../colourSchemes/annotationColouring.html">Annotation
+ Colouring</a>.
</em><br></li>
<li><strong>By RNA Helices</strong><br> <em>Colours
the helices of an RNA alignment loaded from a Stockholm file.
provided in this menu.</strong></li>
<li><strong>Pairwise Alignments</strong><br> <em>Applies
Smith and Waterman algorithm to selected sequences. See <a
- href="../calculations/pairwise.html"
- >pairwise alignments</a>.
+ href="../calculations/pairwise.html">pairwise
+ alignments</a>.
</em><br></li>
<li><strong>Principal Component Analysis</strong><br> <em>Shows
a spatial clustering of the sequences based on similarity
scores calculated with the alignment. See <a
- href="../calculations/pca.html"
- >Principal Component Analysis</a>.
+ href="../calculations/pca.html">Principal
+ Component Analysis</a>.
</em> <br></li>
<li><strong>Extract Scores ... (optional)</strong><br> <em>This
option is only visible if Jalview detects one or more
or elsewhere. You need a continuous network connection in order to
use these services through Jalview.</p>
<ul>
- <li><strong>Alignment</strong><br />
- <em> Align the currently selected sequences or all sequences
- in the alignment, or re-align unaligned sequences to the
- aligned sequences. Entries in this menu provide access to the
- various alignment programs supported by <a
- href="../webServices/JABAWS.html"
- >JABAWS</a>. See the <a href="../webServices/msaclient.html">Multiple
- Sequence Alignment webservice client</a> entry for more
- information.
+ <li><strong>Alignment</strong><br /> <em> Align the
+ currently selected sequences or all sequences in the
+ alignment, or re-align unaligned sequences to the aligned
+ sequences. Entries in this menu provide access to the various
+ alignment programs supported by <a
+ href="../webServices/JABAWS.html">JABAWS</a>. See the
+ <a href="../webServices/msaclient.html">Multiple Sequence
+ Alignment webservice client</a> entry for more information.
</em></li>
<li><strong>Secondary Structure Prediction</strong>
<ul>
<li><strong>Multi-Harmony</strong><br> <em>Performs
functional residue analysis on a protein family alignment
with sub-families defined on it. See the <a
- href="../webServices/shmr.html"
- >Multi-Harmony service</a> entry for more information.
+ href="../webServices/shmr.html">Multi-Harmony
+ service</a> entry for more information.
</em></li>
</ul></li>
</ul></li>
<body>
<p>
- <strong>Alignment Window Annotations Menu</strong> <em>Since Jalview
- 2.8.2</em>
+ <strong>Alignment Window Annotations Menu</strong> <em>Since
+ Jalview 2.8.2</em>
</p>
<ul>
<li><strong>Show Alignment Related</strong><em><br>
example, Consensus).</em></li>
<li><em>You can also selectively show or hide annotations
from the <a href="./popupMenu.html">Popup</a> or <a
- href="../features/annotation.html"
- >Annotation</a> menus.
+ href="../features/annotation.html">Annotation</a> menus.
</em></li>
<li><strong>Sort by Sequence</strong><em><br>Sort
sequence-specific annotations by sequence order in the alignment
<ul>
<li><strong>Annotation Label Popup Menu</strong><br> <em>This
menu is opened by clicking anywhere on the annotation row labels
- area (below the sequence ID area).</em>
- <br/><em><strong>Mac Users:</strong> pressing CTRL whilst clicking
- the mouse/track pad is the same as a right-click. See your
- system's settings to configure your track-pad's corners to
- generate right-clicks.</em>
+ area (below the sequence ID area).</em> <br />
+ <em><strong>Mac Users:</strong> pressing CTRL whilst clicking
+ the mouse/track pad is the same as a right-click. See your
+ system's settings to configure your track-pad's corners to
+ generate right-clicks.</em>
<ul>
<li><strong>Add New Row</strong><br> <em>Adds a
new, named annotation row (a dialog box will pop up for you
</ul></li>
<li><strong>Pairwise Alignments</strong><br> <em>Applies
Smith and Waterman algorithm to selected sequences. See <a
- href="../calculations/pairwise.html"
- >pairwise alignments</a>.
+ href="../calculations/pairwise.html">pairwise
+ alignments</a>.
</em><br></li>
<li><strong>Principal Component Analysis</strong><br> <em>Shows
a spatial clustering of the sequences based on similarity scores
calculated over the alignment.. See <a
- href="../calculations/pca.html"
- >Principal Component Analysis</a>.
+ href="../calculations/pca.html">Principal Component
+ Analysis</a>.
</em> <br></li>
<li><strong>Extract Scores ... (optional)</strong><br> <em>This
option is only visible if Jalview detects one or more
parsed into sequence associated annotation which can then be
used to sort the alignment via the Sort by→Score menu.
</em> <br></li>
- <li><strong>Translate as cDNA</strong> (not applet)<br>
- <em>This option is visible for nucleotide alignments. Selecting
- this option shows the DNA's calculated protein product in a new
- <a href="../features/splitView.html">split frame</a> window.
- Note that the translation is not frame- or intron-aware; it
- simply translates all codons in each sequence, using the
- standard <a href="../misc/geneticCode.html">genetic code</a>
- (any incomplete final codon is discarded). You can perform this
- action on the whole alignment, or selected rows, columns, or
- regions.
+ <li><strong>Translate as cDNA</strong> (not applet)<br> <em>This
+ option is visible for nucleotide alignments. Selecting this
+ option shows the DNA's calculated protein product in a new <a
+ href="../features/splitView.html">split frame</a> window. Note
+ that the translation is not frame- or intron-aware; it simply
+ translates all codons in each sequence, using the standard <a
+ href="../misc/geneticCode.html">genetic code</a> (any incomplete
+ final codon is discarded). You can perform this action on the
+ whole alignment, or selected rows, columns, or regions.
</em> <br></li>
<li><strong>Reverse, Reverse Complement</strong> (not applet)<br>
- <em>These options are visible for nucleotide alignments. Selecting them adds the reverse (or reverse complement)
- of the sequences (or selected region) as new sequences in the alignment. To try this out, add this sequence and
- perform 'Reverse Complement' followed by 'Translate as cDNA':
- <br><small>
- Seq GTCATTTGCGCGTGTTGATTATTCGGACCGCTCCACTTCCCTTTACTCGTGCGTTCAATTGATTTAATCCTC
- TGGGGGGGCTCTGGTTTACATAGCTTAAATCTATTCCATTCAAGGAAGCTCATG</small>
+ <em>These options are visible for nucleotide alignments.
+ Selecting them adds the reverse (or reverse complement) of the
+ sequences (or selected region) as new sequences in the
+ alignment. To try this out, add this sequence and perform
+ 'Reverse Complement' followed by 'Translate as cDNA': <br>
+ <small> Seq
+ GTCATTTGCGCGTGTTGATTATTCGGACCGCTCCACTTCCCTTTACTCGTGCGTTCAATTGATTTAATCCTC
+ TGGGGGGGCTCTGGTTTACATAGCTTAAATCTATTCCATTCAAGGAAGCTCATG</small>
</em> <br></li>
<li><strong>Get Cross-References</strong> (not applet)<br>
- <em>This option is visible where sequences have
+ <em>This option is visible where sequences have
cross-references to other standard databases; for example, an
EMBL entry may have cross-references to one or more UNIPROT
entries. Select the database to view all cross-referenced
</strong><em>All columns which only contain gap characters
("-", ".") will be deleted.<br> You may
set the default gap character in <a
- href="../features/preferences.html"
- >preferences</a>.
+ href="../features/preferences.html">preferences</a>.
</em></li>
<li><strong>Remove All Gaps (Control Shift E)</strong><br>
<em>Gap characters ("-", ".") will be
deleted from the selected area of the alignment. If no selection
is made, ALL the gaps in the alignment will be removed.<br>
You may set the default gap character in <a
- href="../features/preferences.html"
- >preferences</a>.
+ href="../features/preferences.html">preferences</a>.
</em></li>
<li><strong>Remove Redundancy (Control D)<br>
</strong><em>Selecting this option brings up a window asking you to
dialog window in which you can select known ids from UniProt,
EMBL, EMBLCDS, PDB, PFAM, or RFAM databases using Web Services
provided by the European Bioinformatics Institute. See <a
- href="../features/seqfetch.html"
- >Sequence Fetcher</a>
+ href="../features/seqfetch.html">Sequence Fetcher</a>
</em>.</li>
<li><strong>Add Sequences</strong><em><br> Add
sequences to the visible alignment from file, URL, or cut &
<li><strong>Export Image</strong> <em><br> Creates an
alignment graphic with the current view's annotation, alignment
background colours and group colours. If the alignment is <a
- href="../features/wrap.html"
- >wrapped</a>, the output will also be wrapped and will have the same
- visible residue width as the open alignment. </em>
+ href="../features/wrap.html">wrapped</a>, the output will
+ also be wrapped and will have the same visible residue width as
+ the open alignment. </em>
<ul>
<li><strong>HTML<br>
</strong><em>Create a <a href="../io/export.html">web page</a> from
</em></li>
<li><strong>Load Features / Annotations<br>
</strong><em>Load files describing precalculated <a
- href="../features/featuresFormat.html"
- >sequence features</a> or <a
- href="../features/annotationsFormat.html"
- >alignment annotations</a>.
+ href="../features/featuresFormat.html">sequence
+ features</a> or <a href="../features/annotationsFormat.html">alignment
+ annotations</a>.
</em></li>
<li><strong>Close (Control W)</strong><br> <em>Close
the alignment window. Make sure you have saved your alignment
for faster alignment rendering. </em></em></li>
<li><strong>Wrap<br>
</strong><em>When ticked, the alignment display is "<a
- href="../features/wrap.html"
- >wrapped</a>" to the width of the alignment window. This is
- useful if your alignment has only a few sequences to view its full
- width at once.<br> Additional options for display of sequence
- numbering and scales are also visible in wrapped layout mode:
+ href="../features/wrap.html">wrapped</a>" to the width
+ of the alignment window. This is useful if your alignment has only
+ a few sequences to view its full width at once.<br>
+ Additional options for display of sequence numbering and scales
+ are also visible in wrapped layout mode:
</em>
<ul>
<li><strong>Scale Left</strong><br> <em>Show the
<strong>WARNING</strong>: This cannot be undone.
</em></li>
<li><strong><a
- href="../features/columnFilterByAnnotation.html"
- >Select/Hide Columns by Annotation</a></strong> <br /> <em>Select or
- Hide columns in the alignment according to secondary structure,
- labels and values shown in alignment annotation rows. </em></li>
+ href="../features/columnFilterByAnnotation.html">Select/Hide
+ Columns by Annotation</a></strong> <br /> <em>Select or Hide columns
+ in the alignment according to secondary structure, labels and
+ values shown in alignment annotation rows. </em></li>
</ul>
</body>
</html>
<li><strong>Save Project</strong><br> <em>Saves
all currently open alignment windows with their current view
settings and any associated trees, as a <a
- href="../features/jalarchive.html"
- >Jalview Archive</a> (which has a .jar extension).
+ href="../features/jalarchive.html">Jalview
+ Archive</a> (which has a .jar extension).
</em></li>
<li><strong>Load Project</strong><br> <em>Loads
Jalview archives <strong>only</strong>.
window to the top of the pile when it is selected.
<ul>
<li><strong>Close All</strong><br> Close all
- alignment and analysis windows.<br>
- <strong>Note: This will erase all alignments from
- memory, and cannot be undone!</strong></li>
+ alignment and analysis windows.<br> <strong>Note:
+ This will erase all alignments from memory, and cannot be
+ undone!</strong></li>
<li><strong>Raise Associated Windows</strong><br>
Bring all windows associated with the current alignment to
the top of the pile.</li>
<p>
The <a href="popupMenu.html">Popup Menus</a> are opened by clicking
with the right mouse button in the alignment display area or on a
- sequence label in the alignment window.<br />
- <em><strong>Mac Users:</strong> pressing CTRL whilst clicking
- the mouse/track pad is the same as a right-click. See your
- system's settings to configure your track-pad's corners to
- generate right-clicks.</em>
+ sequence label in the alignment window.<br /> <em><strong>Mac
+ Users:</strong> pressing CTRL whilst clicking the mouse/track pad is the
+ same as a right-click. See your system's settings to configure
+ your track-pad's corners to generate right-clicks.</em>
</p>
<p>
The <a href="alwannotationpanel.html">Annotations Menu</a> is opened
<body>
<p>
- <strong>Popup Menu</strong><br> <em>This menu is visible when
- right clicking either within a selected region on the alignment or
- on a selected sequence name. It may not be accessible when in
- 'Cursor Mode' (toggled with the F2 key).</em><br /> <em><strong>Mac Users:</strong> pressing CTRL whilst clicking
- the mouse/track pad is the same as a right-click. See your
- system's settings to configure your track-pad's corners to
- generate right-clicks.</em>
+ <strong>Popup Menu</strong><br> <em>This menu is visible
+ when right clicking either within a selected region on the
+ alignment or on a selected sequence name. It may not be accessible
+ when in 'Cursor Mode' (toggled with the F2 key).</em><br /> <em><strong>Mac
+ Users:</strong> pressing CTRL whilst clicking the mouse/track pad is the
+ same as a right-click. See your system's settings to configure
+ your track-pad's corners to generate right-clicks.</em>
</p>
<ul>
<li><strong>Selection</strong>
<li><a name="sqreport"><strong>Sequence
Details...<br>
</strong></a><em>(Since Jalview 2.8)<br>Open an <a
- href="../io/exportseqreport.html"
- >HTML report containing the annotation and database cross
- references</a> normally shown in the sequence's tooltip.
+ href="../io/exportseqreport.html">HTML report
+ containing the annotation and database cross references</a> normally
+ shown in the sequence's tooltip.
</em></li>
<li><strong>Show Annotations...<br>
</strong><em>Choose to show (unhide) either All or a selected type
</strong><em>The selection area will be output to a a text window in
the selected alignment format. </em></li>
<li><strong><a
- href="../features/creatinFeatures.html"
- >Create Sequence Feature...</a></strong><br> <em>Opens the
- dialog box for creating sequence features over the currently
- selected region on each selected sequence.</em></li>
+ href="../features/creatinFeatures.html">Create
+ Sequence Feature...</a></strong><br> <em>Opens the dialog box
+ for creating sequence features over the currently selected
+ region on each selected sequence.</em></li>
<li><strong>Create Group<br>
</strong><em>This will define a new group from the current
selection.</em><strong> </strong></li>
<li><a name="sqreport"><strong>Sequence
Details ...<br>
</strong></a><em>(Since Jalview 2.8)<br>Open an <a
- href="../io/exportseqreport.html"
- >HTML report containing the annotation and database cross
- references</a> normally shown in the sequence's tooltip.
+ href="../io/exportseqreport.html">HTML report
+ containing the annotation and database cross references</a>
+ normally shown in the sequence's tooltip.
</em></li>
<li><strong>Edit Name/Description<br>
</strong><em>You may edit the name and description of each sequence.
and sequence description to be entered. Press OK to accept
your edit. To save sequence descriptions, you must save in
Fasta, PIR or Jalview File format.</em></li>
- <li><strong>Add <a href="../features/annotation.html#seqannots">Reference Annotations</a></strong><br>
- <em>When enabled, copies any available alignment
- annotation for this sequence to the current view.</em></li>
+ <li><strong>Add <a
+ href="../features/annotation.html#seqannots">Reference
+ Annotations</a></strong><br> <em>When enabled, copies any
+ available alignment annotation for this sequence to the
+ current view.</em></li>
<li><strong>Set as Reference</strong> or <strong>Unmark
- as Reference</strong><br /> Sets or unsets the reference sequence for
- the the alignment.</li>
+ as Reference</strong><br /> Sets or unsets the reference sequence
+ for the the alignment.</li>
<li><strong>Represent Group With (Sequence Id)</strong><br>
<em>All sequences in the current selection group will be
Connections tab.<br> Since Jalview 2.4, links will
also be made for database cross references (where the
database name exactly matches the link name set up in <a
- href="../features/preferences.html"
- >Preferences</a>). <br>Since Jalview 2.5, links are also
- shown for non-positional sequence features attached to the
- sequence, and any regular-expression based URL links that
- matched the description line.
+ href="../features/preferences.html">Preferences</a>).
+ <br>Since Jalview 2.5, links are also shown for
+ non-positional sequence features attached to the sequence,
+ and any regular-expression based URL links that matched
+ the description line.
</em><strong><br> </strong></li>
</ul></li>
<li><strong>3D Structure Data...</strong> </strong><em>This menu is
visible when you right-click on a sequence name. When this
option is clicked, Jalview will open the <a
- href="../features/structurechooser.html">'Structure
- Chooser' </a>, which allows you to discover and view 3D structures
- for the current selection. For more info, see <a
+ href="../features/structurechooser.html">'Structure Chooser'
+ </a>, which allows you to discover and view 3D structures for the
+ current selection. For more info, see <a
href="../features/viewingpdbs.html">viewing PDB structures</a>.
</em></li>
- <li><strong>VARNA 2D Structure</strong><br />
- <em> If the sequence or alignment has RNA structure, then <strong>VARNA
+ <li><strong>VARNA 2D Structure</strong><br /> <em> If the
+ sequence or alignment has RNA structure, then <strong>VARNA
2D Structure</strong> entries will also be present enabling you to open
a linked view of the RNA structure in <a
- href="../features/varna.html"
- >VARNA</a>.
+ href="../features/varna.html">VARNA</a>.
</em></li>
<li><a name="hideinserts"><strong>Hide Insertions</strong></a><br />
<em>Hides columns containing gaps in the current sequence or
elsewhere. You need a continuous network connection in order to use
these services through Jalview.</p>
<ul>
- <li><strong>Alignment</strong><br />
- <em> Align the currently selected sequences or all sequences in
- the alignment, or re-align unaligned sequences to the aligned
- sequences. Entries in this menu provide access to the various
- alignment programs supported by <a
- href="../webServices/JABAWS.html"
- >JABAWS</a>. See the <a href="../webServices/msaclient.html">Multiple
+ <li><strong>Alignment</strong><br /> <em> Align the
+ currently selected sequences or all sequences in the alignment,
+ or re-align unaligned sequences to the aligned sequences.
+ Entries in this menu provide access to the various alignment
+ programs supported by <a href="../webServices/JABAWS.html">JABAWS</a>.
+ See the <a href="../webServices/msaclient.html">Multiple
Sequence Alignment webservice client</a> entry for more
information.
</em></li>
<li><strong>Multi-Harmony</strong><br> <em>Performs
functional residue analysis on a protein family alignment
with sub-families defined on it. See the <a
- href="../webServices/shmr.html"
- >Multi-Harmony service</a> entry for more information.
+ href="../webServices/shmr.html">Multi-Harmony
+ service</a> entry for more information.
</em></li>
</ul></li>
</ul>
way:
<ul>
<li><em>RFAM</em> - Sequences can be <a
- href="../features/seqfetch.html"
- >fetched</a> from the RFAM database by accession number or ID.</li>
+ href="../features/seqfetch.html">fetched</a> from the RFAM
+ database by accession number or ID.</li>
<li><em>Stockholm files</em> - WUSS (or VIENNA) dot-bracket
notation found in the secondary structure annotation line will be
imported as sequence or alignment associated secondary structure
annotation.</li>
<li><em>Clustal files</em> - certain RNA alignment programs,
- such as <a
- href="http://rna.informatik.uni-freiburg.de/LocARNA"
- >LocaRNA</a> output consensus RNA secondary structure lines in the
- line normally reserved for the Clustal consensus line in a clustal
+ such as <a href="http://rna.informatik.uni-freiburg.de/LocARNA">LocaRNA</a>
+ output consensus RNA secondary structure lines in the line
+ normally reserved for the Clustal consensus line in a clustal
file.</li>
<li><em>RNAML</em> Jalview can import RNAML files containing
sequences and extended secondary structure annotation derived from
per-sequence secondary structure is available).</li>
</ul>
<p>
- <strong>Pseudo-knots</strong><br /> Jalview 2.8.2 introduced limited
- support for working with structures including pseudoknots. Where
- possible, extended WUSS symbols (e.g. different types of
+ <strong>Pseudo-knots</strong><br /> Jalview 2.8.2 introduced
+ limited support for working with structures including pseudoknots.
+ Where possible, extended WUSS symbols (e.g. different types of
parentheses, or upper and lower case letters) are preserved when
parsing RNA structure annotation and will be shaded differently when
displayed in the structure.<br /> Extended WUSS annotation is also
<li><em>HTTP logs on the Jalview website</em><br> We
record IP addresses of machines which access the web site, either
via the browser when downloading the application, or when the
- Jalview Desktop user interface is launched.<br>
- <br>
+ Jalview Desktop user interface is launched.<br> <br>
<ul>
<li><i>The JNLP file at
www.jalview.org/webstart/jalview.jnlp is retrieved to
interactions with the public Jalview web services are
logged, but we delete all job data (input data and results)
after about two weeks.</i></li>
- </ul>
- <br></li>
+ </ul> <br></li>
<li><em>Google Analytics</em><br> Since Jalview 2.4.0b2,
the Jalview Desktop records usage data with Google Analytics via
the <a href="http://code.google.com/p/jgoogleanalytics/">JGoogleAnalytics</a>
run Jalview in 'headless mode' via the command line, then the
program shouldn't try to contact any of the web servers mentioned
above (if it does, then it's a bug!). You can also specify some <a
- href="features/commandline.html"
- >command line options</a> to disable the questionnaire and usage
- statistics check. Finally, the <a
- href="features/preferences.html#connections"
- >Connections Tab</a> of the Jalview preferences contains options for
- controlling the submission of usage statistics.
+ href="features/commandline.html">command line options</a> to
+ disable the questionnaire and usage statistics check. Finally, the <a
+ href="features/preferences.html#connections">Connections
+ Tab</a> of the Jalview preferences contains options for controlling
+ the submission of usage statistics.
<p>
<strong>Other Web Clients in Jalview</strong><br> The Jalview
desktop is intended to make it easier to interact with web-based
and <strong>A</strong>nalysis of <strong>Molecular</strong> <strong>S</strong>equences,
<strong>Alignements</strong> and <strong>S</strong>tructures).
Currently, the only other VAMSAS enabled application is <a
- href="http://www.topali.org"
- >TOPALi</a> - a user friendly program for phylogenetics and
- evolutionary analysis.
+ href="http://www.topali.org">TOPALi</a> - a user friendly
+ program for phylogenetics and evolutionary analysis.
<p>
VAMSAS enabled applications access a shared bioinformatics dataset
containing sequences, alignments, annotation and trees, which can be
represented by an XML document analogous to a <a
- href="../features/jalarchive.html"
- >Jalview Project Archive</a>.
+ href="../features/jalarchive.html">Jalview Project
+ Archive</a>.
</p>
<br>
<strong>Connecting to a VAMSAS session</strong>
The majority of these scores were described by Valdar in 2002
(Scoring residue conservation. <em>Proteins: Structure,
Function, and Genetics</em> 43(2): 227-241. <a
- href="http://www.ncbi.nlm.nih.gov/pubmed/12112692"
- >PubMed</a> or available on the <a
- href="http://valdarlab.unc.edu/publications.html"
- >Valdar Group publications page</a>), but the SMERFs score was
- developed later and described by Manning et al. in 2008 (<a
- href="http://www.biomedcentral.com/1471-2105/9/51"
- >BMC Bioinformatics 2008, 9:51 doi:10.1186/1471-2105-9-51</a>).
+ href="http://www.ncbi.nlm.nih.gov/pubmed/12112692">PubMed</a>
+ or available on the <a
+ href="http://valdarlab.unc.edu/publications.html">Valdar
+ Group publications page</a>), but the SMERFs score was developed later
+ and described by Manning et al. in 2008 (<a
+ href="http://www.biomedcentral.com/1471-2105/9/51">BMC
+ Bioinformatics 2008, 9:51 doi:10.1186/1471-2105-9-51</a>).
</p>
<p>
<strong>Enabling and disabling AACon calculations</strong><br />
<strong>Configuring which AACon calculations are performed</strong><br />
The <strong>Web Services→Conservation→Change
AACon Settings ...</strong> menu entry will open a <a
- href="webServicesParams.html"
- >web services parameter dialog</a> for the currently configured AACon
- server. Standard presets are provided for quick and more expensive
- conservation calculations, and parameters are also provided to
- change the way that SMERFS calculations are performed.<br /> <em>AACon
- settings for an alignment are saved in <a
- href="../features/jalarchive.html"
- >Jalview projects</a> along with the latest calculation results.
+ href="webServicesParams.html">web services parameter
+ dialog</a> for the currently configured AACon server. Standard presets
+ are provided for quick and more expensive conservation calculations,
+ and parameters are also provided to change the way that SMERFS
+ calculations are performed.<br /> <em>AACon settings for an
+ alignment are saved in <a href="../features/jalarchive.html">Jalview
+ projects</a> along with the latest calculation results.
</em>
</p>
<p>
the <a href="webServicesPrefs.html">Web Services Preferences
Panel</a>, and detailed information about a particular service is
available from the help text and web pages accessible from its <a
- href="webServicesParams.html"
- >job parameters dialog box</a>.
+ href="webServicesParams.html">job parameters dialog box</a>.
</p>
<p>
<strong>Obtaining JABAWS</strong><br> One of the aims of JABAWS
stand-alone execution of analysis programs, or as a job submission
engine - enabling larger numbers of jobs to be handled. If you would
like to download and install JABAWS for your own use, please go to <a
- href="http://www.compbio.dundee.ac.uk/jabaws"
- >http://www.compbio.dundee.ac.uk/jabaws</a> for more information.
+ href="http://www.compbio.dundee.ac.uk/jabaws">http://www.compbio.dundee.ac.uk/jabaws</a>
+ for more information.
</p>
<p>
<strong>Configuring your own JABAWS services for use by
Jalview</strong><br> Once you have downloaded and installed JABAWS,
and verified it is working, all that is needed is to add the URL for
your JABAWS server(s) to the list in the <a
- href="webServicesPrefs.html"
- >Web Services Preferences Panel</a>. After adding your service and
- saving your preferences or hitting the 'refresh web services'
- button, you should be able to submit jobs to the server via the
- alignment window's web services menu. Your JABAWS servers list is
- stored in your Jalview preferences, so you will only have to
- configure Jalview once for each new server.
+ href="webServicesPrefs.html">Web Services Preferences
+ Panel</a>. After adding your service and saving your preferences or
+ hitting the 'refresh web services' button, you should be able to
+ submit jobs to the server via the alignment window's web services
+ menu. Your JABAWS servers list is stored in your Jalview
+ preferences, so you will only have to configure Jalview once for
+ each new server.
</p>
<p>
<em>Support for accessing JABAWS servers was introduced in
Gruber, and Peter F. Stadler, <em>RNAalifold: Improved
consensus structure prediction for RNA alignments</em> (BMC
Bioinformatics, 9:474, 2008). Download the paper at <a
- href="http://www.biomedcentral.com/1471-2105/9/474"
- >http://www.biomedcentral.com/1471-2105/9/474</a>.
+ href="http://www.biomedcentral.com/1471-2105/9/474">http://www.biomedcentral.com/1471-2105/9/474</a>.
</p>
<p>
<strong>Running RNAalifold from Jalview</strong><br />
<Strong>RNAalifold prediction parameters</Strong> <br /> JABAWS and
Jalview only provide access to a selection of the RNAalifold
arguments. For a full description, see the documentation at <a
- href="http://www.tbi.univie.ac.at/RNA/RNAalifold.html"
- >http://www.tbi.univie.ac.at/RNA/RNAalifold.html</a>.
+ href="http://www.tbi.univie.ac.at/RNA/RNAalifold.html">http://www.tbi.univie.ac.at/RNA/RNAalifold.html</a>.
</p>
<p>
<strong>Supported Arguments which give alternate structures</strong>
Calculate an MEA structure where the expected Accuracy is computed
from the base pair probabilities. A more detailed description can be
found in the <strong>RNAfold</strong> program documentation at <a
- href="http://www.tbi.univie.ac.at/RNA/RNAfold.html"
- >http://www.tbi.univie.ac.at/RNA/RNAfold.html</a>.
+ href="http://www.tbi.univie.ac.at/RNA/RNAfold.html">http://www.tbi.univie.ac.at/RNA/RNAfold.html</a>.
</p>
<p>
<strong>Example RNAalifold Structure Annotation rows</strong>
Database references are associated with a sequence are displayed as a
list in the tooltip shown when mousing over its sequence ID. Jalview
uses references for the retrieval of <a
- href="../features/viewingpdbs.html"
- >PDB structures</a> and <a href="../features/dasfeatures.html">DAS
- features</a>, and for retrieving sequence cross-references such as the
- protein products of a DNA sequence.
+ href="../features/viewingpdbs.html">PDB structures</a> and <a
+ href="../features/dasfeatures.html">DAS features</a>, and for
+ retrieving sequence cross-references such as the protein products of a
+ DNA sequence.
</p>
<p>
<strong>Initiating reference retrieval</strong><br> The
and was originally restricted to the identification of valid UniProt
accessions.<br> Essentially, Jalview will try to retrieve records
from a subset of the databases accessible by the <a
- href="../features/seqfetch.html"
- >sequence fetcher</a> using each sequence's ID string (or each string in
- the ID separated by the '∣' symbol).
+ href="../features/seqfetch.html">sequence fetcher</a> using each
+ sequence's ID string (or each string in the ID separated by the
+ '∣' symbol).
</p>
<p>If a record (or set of records) is retrieved by any query derived
from the ID string of a sequence, then the sequence is aligned to the
<ul>
<li>Programs for <a href="msaclient.html">multiple
sequence alignment</a>, made available <em>via</em> <a
- href="JABAWS.html"
- >Java Bioinformatic Analysis Web Service (JABAWS)</a> servers.
+ href="JABAWS.html">Java Bioinformatic Analysis
+ Web Service (JABAWS)</a> servers.
</li>
<li>Jalview SOAP Web Services for <a href="jnet.html">secondary
structure prediction</a> based at the University of Dundee.
</li>
<li>Services for alignment analysis, such as <a
- href="shmr.html"
- >Multi-Harmony</a>.
+ href="shmr.html">Multi-Harmony</a>.
</ul>
<p>
<strong>Web Service Dialog Box</strong>
citation information, and monitors the progress of the
calculation. The cancel button will permanently cancel the job,
but this is only possible for some services.</p> The <a
- href="webServicesPrefs.html"
- >Web Services Preference panel</a> controls the display and appearance
- of the submission and analysis services in the <strong>Web
- Services</strong> menu.
+ href="webServicesPrefs.html">Web Services Preference
+ panel</a> controls the display and appearance of the submission and
+ analysis services in the <strong>Web Services</strong> menu.
</li>
<li>If Jalview encounters problems accessing any services, it
may display a <a href="webServicesPrefs.html#wswarnings">warning
<p>
<strong>More about Jalview's Web Services</strong> <br>
Jalview's distributed computations utilise <a
- href="http://en.wikipedia.org/wiki/SOAP"
- >SOAP</a> and <a
- href="http://en.wikipedia.org/wiki/Representational_State_Transfer"
- >REST</a> web services exposing sequence alignment, analysis, and
- secondary structure prediction programs. Originally, Jalview 2's
- services were maintained by the Barton group at the University of
- Dundee, and ran programs on the Life Sciences High-performance
- Computing Cluster. With the advent of <a
- href="http://www.compbio.dundee.ac.uk/jabaws"
- >JABAWS</a>, however, it is possible for anyone to host Jalview web
- services.
+ href="http://en.wikipedia.org/wiki/SOAP">SOAP</a> and <a
+ href="http://en.wikipedia.org/wiki/Representational_State_Transfer">REST</a>
+ web services exposing sequence alignment, analysis, and secondary
+ structure prediction programs. Originally, Jalview 2's services were
+ maintained by the Barton group at the University of Dundee, and ran
+ programs on the Life Sciences High-performance Computing Cluster.
+ With the advent of <a href="http://www.compbio.dundee.ac.uk/jabaws">JABAWS</a>,
+ however, it is possible for anyone to host Jalview web services.
</p>
</body>
</html>
JPred4: a protein secondary structure prediction server<br /> <em>Nucleic
Acids Research</em>, <strong>Web Server issue</strong> (first
published 15th April 2015)<br /> <a
- href="http://dx.doi.org/10.1093/nar/gkv332"
- >http://dx.doi.org/10.1093/nar/gkv332</a>
+ href="http://dx.doi.org/10.1093/nar/gkv332">http://dx.doi.org/10.1093/nar/gkv332</a>
</li>
<li>Cole C., Barber J.D. and Barton G.J. (2008) The Jpred 3
secondary structure prediction server <em>Nucleic Acids
</p>
<em>JNet annotation created in Jalview 2.8.2 and later versions
can be displayed on other alignments via the <a
- href="../features/annotation.html#seqannots"
- >Add reference annotation</a> Sequence ID popup menu option.
+ href="../features/annotation.html#seqannots">Add reference
+ annotation</a> Sequence ID popup menu option.
</em>
<em>As of Jalview 2.6, the Jnet service accessed accessed via the
'Secondary structure prediction' submenu should be considered a
<li><a href="http://www.drive5.com/muscle">Muscle</a> (version
3.8.31)</li>
<li><a
- href="http://www.tcoffee.org/Projects_home_page/t_coffee_home_page.html"
- >Tcoffee</a> (version 8.99)</li>
+ href="http://www.tcoffee.org/Projects_home_page/t_coffee_home_page.html">Tcoffee</a>
+ (version 8.99)</li>
<li><a href="http://probcons.stanford.edu/">Probcons</a>
(version 1.12)</li>
</ul>
<strong>Multiple Alignments of Sequences with hidden
columns</strong><br> Multiple alignment services are 'column
separable' analysis operations. If the input contains <a
- href="../features/hiddenRegions.html"
- >hidden columns</a> then each visible segment of the input sequence
- set will be submitted for alignment separately, and the results
- concatenated (with the hidden regions preserved) once all alignment
- functions have completed. Each sub-job's state is reported in its
- own tab:
+ href="../features/hiddenRegions.html">hidden columns</a> then
+ each visible segment of the input sequence set will be submitted for
+ alignment separately, and the results concatenated (with the hidden
+ regions preserved) once all alignment functions have completed. Each
+ sub-job's state is reported in its own tab:
<p>
<center>
<strong>Multiple Multiple Sequence Alignment sub jobs
<p>
<strong>The Jalview Desktop RSS News Reader</strong><br /> The
Jalview Desktop includes a built in news reader for the <a
- href="http://www.jalview.org/feeds/desktop/rss"
- >Jalview Desktop News Channel</a>.
+ href="http://www.jalview.org/feeds/desktop/rss">Jalview
+ Desktop News Channel</a>.
</p>
<p>We will use the desktop news channel to keep you informed of
important updates relevant to users of the Jalview desktop, such as
web service outages and user community events.</p>
- <p>The news reader will be launched automatically when you start
- the Desktop if new items are available. Should you want to browse
- older items, however, you can open it manually from the 'Jalview
- news reader' option in the Desktop's <a href="../menus/desktopMenu.html">'Tools' menu</a>.</p><br/>
- <div style="text-align: center;"><img src="jalviewrssreader.gif" width="513"
- height="337" alt="Snapshot of the Jalview Desktop's RSS reader"/></div>
+ <p>
+ The news reader will be launched automatically when you start the
+ Desktop if new items are available. Should you want to browse older
+ items, however, you can open it manually from the 'Jalview news
+ reader' option in the Desktop's <a href="../menus/desktopMenu.html">'Tools'
+ menu</a>.
+ </p>
+ <br />
+ <div style="text-align: center;">
+ <img src="jalviewrssreader.gif" width="513" height="337"
+ alt="Snapshot of the Jalview Desktop's RSS reader" />
+ </div>
- <br/><p>
+ <br />
+ <p>
The <em>Jalview news reader</em> was introduced in <a
- href="http://www.jalview.org/releaseHistory.html#Jalview2.7"
- >Jalview version 2.7</a>. Its implementation is based on <a
- href="http://jswingreader.sourceforge.net/"
- >JSwingReader</a>.
+ href="http://www.jalview.org/releaseHistory.html#Jalview2.7">Jalview
+ version 2.7</a>. Its implementation is based on <a
+ href="http://jswingreader.sourceforge.net/">JSwingReader</a>.
</p>
<br />
<em>If you need to prevent the news-reader opening, then add the
The <strong>Web Services→Disorder</strong> menu in the
alignment window allows access to protein disorder prediction
services provided by the configured <a
- href="http://www.compbio.dundee.ac.uk/jabaws"
- >JABAWS servers</a>. Each service operates on sequences in the
- alignment or currently selected region (<em>since Jalview
- 2.8.0b1</em>) to identify regions likely to be unstructured or
- flexible, or alternately, fold to form globular domains.
+ href="http://www.compbio.dundee.ac.uk/jabaws">JABAWS
+ servers</a>. Each service operates on sequences in the alignment or
+ currently selected region (<em>since Jalview 2.8.0b1</em>) to
+ identify regions likely to be unstructured or flexible, or
+ alternately, fold to form globular domains.
</p>
<p>
Predictor results include both <a
- href="../features/seqfeatures.html"
- >sequence features</a> and sequence associated <a
- href="../features/annotation.html"
- >alignment annotation</a> rows. Features display is controlled from
- the <a href="../features/featuresettings.html">Feature Settings</a>
+ href="../features/seqfeatures.html">sequence features</a> and
+ sequence associated <a href="../features/annotation.html">alignment
+ annotation</a> rows. Features display is controlled from the <a
+ href="../features/featuresettings.html">Feature Settings</a>
dialog box. Clicking on the ID for a disorder prediction annotation
row will highlight or select (if double clicked) the associated
sequence for that row. You can also use the <em>Sequence
Associated</em> option in the <a
- href="../colourSchemes/annotationColouring.html"
- >Colour By Annotation</a> dialog box to colour sequences according to
- the results of predictors shown as annotation rows.
+ href="../colourSchemes/annotationColouring.html">Colour
+ By Annotation</a> dialog box to colour sequences according to the
+ results of predictors shown as annotation rows.
</p>
<p>JABAWS 2.0 provides four disorder predictors which are
described below:</p>
<td>Sequence Feature &<br />Annotation Row
</td>
<td>Predicts loops/coils according to DSSP definition<a
- href="#dsspstates"
- >[1]</a>.<br />Features mark range(s) of residues predicted as
- loops/coils, and annotation row gives raw value for each
- residue. Value over 0.516 indicates loop/coil.
+ href="#dsspstates">[1]</a>.<br />Features mark range(s)
+ of residues predicted as loops/coils, and annotation row gives
+ raw value for each residue. Value over 0.516 indicates
+ loop/coil.
</td>
</tr>
<tr>
<p>
<strong><a name="ronn"></a><a
- href="http://www.strubi.ox.ac.uk/RONN"
- >RONN</a></strong> <em>a.k.a.</em> Regional Order Neural Network<br />This
- predictor employs an approach known as the 'bio-basis' method to
- predict regions of disorder in sequences based on their local
- similarity with a gold-standard set of disordered protein sequences.
- It yields a set of disorder prediction scores, which are shown as
- sequence annotation below the alignment.
+ href="http://www.strubi.ox.ac.uk/RONN">RONN</a></strong> <em>a.k.a.</em>
+ Regional Order Neural Network<br />This predictor employs an
+ approach known as the 'bio-basis' method to predict regions of
+ disorder in sequences based on their local similarity with a
+ gold-standard set of disordered protein sequences. It yields a set
+ of disorder prediction scores, which are shown as sequence
+ annotation below the alignment.
</p>
<table border="1">
<tr>
</p>
<p>
<strong><a name="iupred"></a><a
- href="http://iupred.enzim.hu/Help.php"
- >IUPred</a></strong><br /> IUPred employs an empirical model to estimate
- likely regions of disorder. There are three different prediction
- types offered, each using different parameters optimized for
- slightly different applications. It provides raw scores based on two
- models for predicting regions of 'long disorder' and 'short
- disorder'. A third predictor identifies regions likely to form
- structured domains.
+ href="http://iupred.enzim.hu/Help.php">IUPred</a></strong><br />
+ IUPred employs an empirical model to estimate likely regions of
+ disorder. There are three different prediction types offered, each
+ using different parameters optimized for slightly different
+ applications. It provides raw scores based on two models for
+ predicting regions of 'long disorder' and 'short disorder'. A third
+ predictor identifies regions likely to form structured domains.
</p>
<table border="1">
<tr>
</table>
<p>
<strong><a name="globplot"></a><a
- href="http://globplot.embl.de/"
- >GLOBPLOT</a></strong><br /> Defines regions of globularity or natively
- unstructured regions based on a running sum of the propensity of
- residues to be structured or unstructured. The propensity is
- calculated based on the probability of each amino acid being
- observed within well defined regions of secondary structure or
- within regions of random coil. The initial signal is smoothed with a
- Savitzky-Golay filter, and its first order derivative computed.
- Residues for which the first order derivative is positive are
- designated as natively unstructured, whereas those with negative
- values are structured.<br />
+ href="http://globplot.embl.de/">GLOBPLOT</a></strong><br /> Defines
+ regions of globularity or natively unstructured regions based on a
+ running sum of the propensity of residues to be structured or
+ unstructured. The propensity is calculated based on the probability
+ of each amino acid being observed within well defined regions of
+ secondary structure or within regions of random coil. The initial
+ signal is smoothed with a Savitzky-Golay filter, and its first order
+ derivative computed. Residues for which the first order derivative
+ is positive are designated as natively unstructured, whereas those
+ with negative values are structured.<br />
<table border="1">
<tr>
<td><strong>Name</strong></td>
a protein sequence multiple alignment that has been sub-divided into
groups containing at least two non-identical protein sequences. An
easy way to create groups is to use the built-in <a
- href="../calculations/tree.html"
- >neighbour-joining or UPGMA tree</a> routines to calculate a tree for
- the alignment, and then click on the tree to subdivide the
- alignment.
+ href="../calculations/tree.html">neighbour-joining or
+ UPGMA tree</a> routines to calculate a tree for the alignment, and
+ then click on the tree to subdivide the alignment.
</p>
<p>
The SHMR service operates on the currently selected visible
region(s) of the alignment. Once submitted, a job progress window
will display status information about your job, including a URL
which allows you to visit the status page on the <a
- href="http://zeus.few.vu.nl/programs/shmrwww/"
- >IBIVU SHMR server</a>.
+ href="http://zeus.few.vu.nl/programs/shmrwww/">IBIVU SHMR
+ server</a>.
</p>
<p>When the job is complete, Jalview will automatically open a new
window containing the alignment and groups that were submitted for
analysis, with additional histograms added portraying the SHMR
scores for each column of the sub-grouped alignment.</p>
<p>
- If you use this service in your work, please cite :<br />
- <a name="shmrref" /> Brandt, B.W.*, Feenstra, K.A*. and Heringa, J.
+ If you use this service in your work, please cite :<br /> <a
+ name="shmrref" /> Brandt, B.W.*, Feenstra, K.A*. and Heringa, J.
(2010) Multi-Harmony: detecting functional specificity from sequence
alignment. <a
- href="http://nar.oxfordjournals.org/cgi/content/abstract/gkq415"
- >Nucleic Acids Res. 38: W35-W40.</a> (<em>* joint first authors</em>)
-
+ href="http://nar.oxfordjournals.org/cgi/content/abstract/gkq415">Nucleic
+ Acids Res. 38: W35-W40.</a> (<em>* joint first authors</em>)
<p>
<strong><em>Note:</em></strong> The Multi-Harmony service is
implemented with a prototype of Jalview's RESTful web service client
your web browser. <br> Double-clicking on the ID of a sequence
will open the first URL that can be generated from its sequence ID.
This is often the SRS site, but you can easily configure your own <a
- href="#urllinks"
- >sequence URL links</a>.
+ href="#urllinks">sequence URL links</a>.
</p>
<p>
Other links for a sequence either derived from any other configured
<p>
<strong><a name="urllinks">Configuring URL Links</a></strong> <br>URL
links are defined in the "Connections" tab of the <a
- href="../features/preferences.html"
- >Jalview desktop preferences</a>, or specified as <a
- href="http://www.jalview.org/examples/appletParameters.html#parameters"
- >applet parameters</a>. <br> By default the item "SRS"
- is added to this link menu. This link will show a web page in your
- default browser with the selected sequence id as part of the URL.<br>
+ href="../features/preferences.html">Jalview desktop
+ preferences</a>, or specified as <a
+ href="http://www.jalview.org/examples/appletParameters.html#parameters">applet
+ parameters</a>. <br> By default the item "SRS" is added
+ to this link menu. This link will show a web page in your default
+ browser with the selected sequence id as part of the URL.<br>
In the preferences dialog box, click <strong>new</strong> to add a
new link, and <strong>edit</strong> to modify an existing link, or <strong>delete</strong>
to remove it.<br> You can name the link, this will be displayed
<p>
Some Jalview services, including those provided by <a
- href="JABAWS.html"
- >JABAWS</a>, support a range of parameters and options, enabling you
- to employ the most appropriate settings for the input data. In
- addition to any preset combinations provided by services themselves,
- the Web services parameters dialog box also allows you to create and
- store your own parameter sets, so they can be accessed quickly from
- the presets menu.
+ href="JABAWS.html">JABAWS</a>, support a range of parameters
+ and options, enabling you to employ the most appropriate settings
+ for the input data. In addition to any preset combinations provided
+ by services themselves, the Web services parameters dialog box also
+ allows you to create and store your own parameter sets, so they can
+ be accessed quickly from the presets menu.
</p>
<p>
<Strong>Accessing the parameter dialog box</Strong><br> The
<p>
<center>
<img src="wsparams.gif" align="center" width="480" height="499"
- alt="Analysis Parameters Dialog Box for JABAWS Services"
- > <br> Parameter settings dialog box for JABAWS MAFFT Service
+ alt="Analysis Parameters Dialog Box for JABAWS Services">
+ <br> Parameter settings dialog box for JABAWS MAFFT Service
</center>
</p>
<p>The menu and text box at the top of the dialog box displays the
this), allowing you to provide notes to accompany the parameter set.
The modification of these or any of the option or parameter settings
will enable one or more of the following buttons, that allow you to:
+
<ul>
<li><em>Revert</em> the changes you have made. This will undo
<p>
<center>
<img src="wsprefs.gif" align="center"
- alt="Web Services Preferences Panel" width="571" height="461"
- ><br> Web Services Preference Panel
+ alt="Web Services Preferences Panel" width="571" height="461"><br>
+ Web Services Preference Panel
</center>
</p>
<p>
<center>
<img src="invalidurldialog.gif" align="center"
alt="Web Services Invalid URL Warning dialog box" width="389"
- height="258"
- ><br> Web Services Invalid URL Warning dialog box
+ height="258"><br> Web Services Invalid URL Warning
+ dialog box
</center>
<br>
<strong><em>Note:</em></strong> this warning will be shown if you are
git://source.jalview.org / jalview.git / commitdiff